scholarly journals IDENTIFIKASI TOTAL MIKROBA, CEMARAN Escherichia coli DAN NILAI pH SUSU PASTEURISASI YANG BEREDAR DI KOTA MALANG

2019 ◽  
Vol 7 (1) ◽  
pp. 201
Author(s):  
Oktavia Rahayu Puspitarini ◽  
Nurul Jadid Mubarakati

Malang is a rich culinary city of livestock products such as pasteurized milk. The purposes of this study were analyzed total microbes, contamination of Escherichia coli and pH of pasteurized milk produced by milk store, village unit cooperation milk house and STMJ cafe in Malang. The method of this research were survey and experiment. The samples were took from milk store (P1), village unit cooperation milk house (P2) and STMJ café (P3)  in Malang during 4 days. The data analysis of total microbial and pH using one-way analysis of variance (ANOVA) and continued by Duncan Multiple Range Test (DMRT) while Escherichia coli data was analyzed by descriptive analysis. The results showed that there were a real influence (P<0,05) of milk producers on total microbes and pH. Escherichia coli contaminant from three producers were showed the same results which were less than 3 apm/ml. The conclusions of this study were total microbes and contamination of Escherichia coli original pasteurized milk produced by milk store, village unit cooperation milk house and STMJ cafe under the limit of microbial contamination on food. Microbiological quality in terms of total microbes, and contamination of Escherichia coli pasteurized milk by milk store in Malang City were classified to national standard of pasteurized milk in Indonesia. Keywords: Total Microbes, Escherichia coli, pH, Pasteurized Milk, Malang City

Author(s):  
G. R. Hanum ◽  
S. Ardiansyah

Mangkokan soap (Nothopanax Scutellaium Merr) is made from extract of mangkokan leaf and the material making of soap there are oil, NaOH, alcohol and glycerin. This research is to find out the quality of microbiology and chemical soap of mangkokan leaf extract (Nothopanax Scutellaium Merr) with 90% concentration of mangkokan leaf extract . This research is an experimental research with descriptive of data analysis. The results of this study were microbiological quality of Mangkokan extract soap(Nothopanax Scutellaium Merr) has antibacterial activity to Escherichia coli bacteria and there is no microbial contamination. Chemical quality of Mangkokan Extract Soap (Nothopanax Scutellaium Merr) was tested on free alkali level test 0%, pH value 11,03 and water content value 0,4668%.   Keywords: Escherichia coli, Mangkokan Leaf, Soap.


2012 ◽  
Vol 81 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Irena Svobodová ◽  
Gabriela Bořilová ◽  
Radka Hulánková ◽  
Iva Steinhauserová

Microbial contamination of poultry carcasses can be influenced by many factors during transport and slaughtering. The aim of this study was to evaluate the impact of four processing steps (plucking, evisceration, washing and chilling) on the total viable counts (TVC), counts ofEscherichia coli,Salmonellaspp. andListeriaspp. incidence on broiler carcasses. A total of 160 broiler carcasses originating from one farm were collected during one year period at a Czech slaughterhouse and examined. Both TVC andE. colicounts decreased during processing from 4.6 log cfu/cm2and 3.5 log cfu/cm2to 3.7 log cfu/cm2and 1.8 log cfu/cm2, respectively, with a major impact of washing on TVC and washing and chilling onE. colidecrease (P< 0.001). BothSalmonellaspp. (6 strains) andListeriaspp. (12 strains, none ofL. monocytogenes) were found sporadically in all processing steps followed. However, a decreasing trend was observed inSalmonellacounts andListeriaspp. incidence during the processing. Thus, this study brings new valuable information on the dynamics of microflora during modern poultry processing.


Author(s):  
Febrina Olivia Akerina

Smoked tuna is one of traditional fisheries product in Tobelo, North Halmahera. Tobelos people like this product because of its smoke taste. In Tobelo, there are many producer who sell this product, but information about its quality was unknown. In this research, the researcher wants to know about microbiology aspect of smoked tuna from three traditional market in Tobelo. The result of this research showed that Total Plate Count (TPC) value was from 7,5x1015,35x102 APM/g; smoked tuna from modern Market showed the highest Staphulococcus sp. colony at 1,3x103 CFU/g. colony of Salmonella sp. showed negative value for three different market. The highest fungy colony was 2,5x101 CFU/g from Gotong royong Market, for Escherichia coli analysis, smoked tuna from Gotong Royong Market showed high value at 23 CFU/g, it was exceed Indonesia National Standard.


2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Retno Sri Wahjuni

Enterotoxin Escherichia coli resistant to antibiotics on broiler. The used of antibiotics should be re-evaluated, as well as over Escherichia coli enterotoxin. Plants meniran (Phyllanthus niruri Linn) is a plant that can be used as an alternative prevention and treatment of diseases caused by Escherichia coli enterotoxin. In this research, broilers at the age of 23 days were divided into six groups. Two groups are positive control was infected by Escherichia coli and negative control was not infected by Escherichia coli. Three groups are extract of Phyllanthus niruri Linn. plant that variety in three dosage: 20%, 25%, 30% and antibiotic. After five days treatment the broiler was to take the sample of blood tissue. The data were tested with Annova and continued by Duncan multiple range test. There was a significant difference of immunomodulation activity between treatment group doses 30% has been able to decrease lymphocyte on blood. The result showed doses 30% can be used as immunomodulation to Escherichia coli.Keywords: Phyllanthus niruri Linn. extract, Immunomodulation, Escherichia coli, Broiler, Lymphocyte


2017 ◽  
Vol 19 (2) ◽  
pp. 33
Author(s):  
Sumarno Sumarno ◽  
Hery Widjianto ◽  
Rosinta Kusuma W

<p>This research conducted to determine the exact formula and dosage to increase the uptake of N and increase yield of green mustard. The research conducted at Jumantono dryland research center, Karanganyar and Laboratory  of Chemistry and Soil Fertility, Faculty of Agriculture UNS Solo. This study uses a randomized complete block design (RCBD) using 5 formulas as the first treatment factor and 4 dosages as second treatment factor. The data analysis used F test level of 5% and if significant continued by DMRT (Duncan Multiple Range Test) level of 5%. The variables of this research were weight of wet stover, weight of dry stover, N total and absorption of N. The results showed that the combination of dosage and formula of natural fertilizers can increase N uptake and yield of mustard on the ground Alfisols.</p>


Author(s):  
Jiaqi Lan ◽  
Shuo Yang ◽  
Yu Wang ◽  
Nan Guo ◽  
Xu Liu ◽  
...  

In this study, we evaluated the microbial contamination status of cold dishes consumed by residents of Jilin Province and investigated to determine the incidence of four pathogenic bacteria in cold dishes. A total of 300 samples of cold dishes including meat, vegetable and mixed products, were collected from three different purchasing places: supermarkets, farmers' markets and mobile vendors. Live bacteria were isolated using conventional culture methods. After separation, a quick and easy polymerase chain reaction (PCR) was used to detect Listeria monocytogenes , Staphylococcus aureus , Enterotoxic Escherichia coli  and Salmonella . The results showed that the total number of microbial colonies in the vegetable samples exceeded the standard rate of 8%, and the total number of microbial colonies in the meat and mixed samples did not exceed the standard. The total microbial colony count exceeded the standard in all three different procurement sites, with the highest exceedance of 7.4% in the mobile vendor sites. The detection rates of Enterotoxigenic Escherichia coli , Staphylococcus aureus , L. monocytogenes  and Salmonella  among the four pathogenic bacteria detected in all samples were 4.3%, 3.3%; 3.0%; and 1.0%, respectively. This study can be used to qualitatively assess the microbiological quality associated with cold dishes. It provides data to support the detection of possible food safety problems.


2020 ◽  
Vol 3 (2) ◽  
pp. 207
Author(s):  
Muh. Ridho Syafarullah Kadir ◽  
Asnani Asnani ◽  
Suwarjoyowirayatno Suwarjoyowirayatno

The Quality of Rebon Shrimp Paste (Acetes Indicus) Traded in Some Markets of Kendari CityABSTRACT         The aim of this study was to determine the sensory quality, chemical quality, and microbiological quality of rebon shrimp paste traded in several traditional markets in Kendari City. Data analysis of the research used descriptive analysis. samples are taken at Mandonga market and Kendari City Central Market. Based on the sensory analysis (appearance, smell, taste, texture), it shows that the Mandonga market is the highest. The highest chemical analysis of rebon shrimp paste are water P2: 43.73%, ash P1: 23.79, and protein p1: 23.75%. The total bacterial analysis ranged from 1.73 Log CFU / gr-3.33 Log CFU / gr.Keywords: Shrimp Rebon, Paste, Kendari market ABSTRAKTujuan penelitian ini untuk mengetahui mutu sensori, mutu kimia dan mutu mikrobiologi terasi udang rebon yang diperdagangkan dibeberapa pasar tradisional Kota Kendari. Analisis data penelitian menggunakan analisis deskriptif yaitu analisis yang dilakukan untuk menilai karakteristik dari sebuah data. Pengambilan sampel yang dilakukan ialah Pasar Mandonga dan pasar sentral Kota kendari. Berdasarkan analisis sensori (Kenampakan, Bau, Rasa, Tekstur) menunjukan bahwa pasar mandonga yang tertinggi. Analisis kimia kadar air tertinggi P2:43,73%, kadar abu tertinggi P1:23,79, dan kadar protein tertinggi p1:23,75%. Analisis total bakteri berkisar 1,73 Log CFU/gr-3,33 Log CFU/gr.Kata kunci: Udang Rebon, Terasi, Pasar Kendari


2013 ◽  
Vol 76 (5) ◽  
pp. 843-848 ◽  
Author(s):  
PALIZ KOOHY-KAMALY-DEHKORDY ◽  
HOUSHANG NIKOOPOUR ◽  
FARIDEH SIAVOSHI ◽  
MOHAMMADREZA KOUSHKI ◽  
ALIREZA ABADI

The microbiological quality of 351 samples of nine types of spices including black pepper, caraway, cinnamon, cow parsnip, curry powder, garlic powder, red pepper, sumac, and turmeric, collected from retail shops in Tehran during 2007, was determined. The numbers of aerobic mesophilic bacteria, Escherichia coli, and molds exceeded Iran's National Standard limits, at 63.2% (&gt;5 × 105 CFU/g), 23.4% (&gt;0.3 MPN/g), and 21.9% (&gt;5 × 103 CFU/g) of the studied samples, respectively. Coliform contamination was more than 103 MPN/g in 24.8% of samples. High contamination of retail spices is considered an indication of environmental or fecal contamination due to unhygienic practices in their production. Use of spices with high microbial content could increase the chance of food spoilage and transmission of foodborne pathogens. Accordingly, application of food safety measurements to reduce microbial counts in spices is strongly recommended.


Agrivet ◽  
2018 ◽  
Vol 24 (1) ◽  
Author(s):  
Fitria Nugraheni Sukmawati ◽  
Dodi Kastono ◽  
Rohlan Rogomulyo

The first aim of this research was to know if brain used on weeds control and changed manual weeds control. The second aim was to know the optimum dosage of bran in any frequency applications on weeds control to increase rice yield. Randomized Complete Block Designe (RCBD) was used and replicated in a three blocks. Analysis of variance was used for data analysis, and than followed with Duncan Multiple Range Test (DMRT) if there was a significant different. Orthogonal Contrast Test was used to know the best treatment in controlled weeds growth. Trend Comparisson Test was used to know the optimum dosage of bran in any frequency applications on weeds control to increase rice yield. The result of this research show that bran could suppress weeds growth and changed manual weeds control, however yet increase rice yield 250 kg/ha of bran wich once and twice application were recommended and treatment combination of 143,33 kg/ha at third application was reach as best combination ti increase rice yield, up to 4,61 ton/ha.Key words: weeds control, manual, bran


2019 ◽  
Vol 4 (2) ◽  
pp. 108
Author(s):  
Lia Saputri ◽  
Agustina Intan Niken Tari ◽  
Sri Hartati

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian yoghurt berpotensi prebiotik dengan penambahan ekstrak ubi jalar ungu sebagai antidiare dan mengetahui performa kesehatan tikus sebagai hewan coba. Tikus yang digunakan adalah tikus putih galur Sparague dawley umur 1,5 bulan. Parameter performa kesehatan tikus meliputi kenaikan berat badan, konsumsi pakan dan efisiensi pakan sedangkan parameter antidiare adalah kadar air feses.  Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 4 faktor perlakuan yaitu Y- = tikus tanpa perlakuan yoghurt prebiotik dan (Enthero pathogenic Escherichia coli) EPEC, Y1 = tikus dengan perlakuan yoghurt prebiotik, Y2 = tikus dengan perlakuan yoghurt prebiotik dan EPEC, Y+ = tikus dengan perlakuan EPEC, setiap perlakuan diulang 5 kali. Data yang diperoleh dianalisis secara statistik dengan Anova. Apabila terdapat beda nyata antar perlakuan dilanjutkan dengan uji DMRT (Duncan Multiple Range Test) pada taraf signifikansi 5%.  Hasil penelitian ini menunjukkan bahwa pemberian yoghurt berpotensi prebiotik memberikan pengaruh yang nyata terhadap konsumsi pakan, berat badan, efisiensi pakan dan kadar air feses pada tikus percobaan.  Kenaikan berat badan dan efisiensi pakan menunjukkan hasil yang tinggi pada tikus yang tidak terinfeksi EPEC dan menunjukkan hasil yang rendah pada tikus yang terinfeksi EPEC. Kadar air feses dan konsumsi pakan menunjukkan hasil yang tinggi pada tikus yang terinfeksi EPEC dan menunjukkan hasil yang rendah pada tikus yang tidak terinfeksi EPEC. Kata kunci : yoghurt, yoghurt prebiotik dan antidiare.


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