scholarly journals ISOLASI LIKOPEN DARI BUAH TOMAT (Solanum lycopersicum L) dan UJI AKTIVITAS LIKOPEN TERHADAP BAKTERI Salmonella thypi

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Earlyna Sinthia Dewi ◽  
Aliefman Hakim ◽  
Lalu Rudyat Telly Savalas
Keyword(s):  
Fourier Transform ◽  
Wave Length ◽  
Extraction Process ◽  
Positive Control ◽  
Negative Control ◽  
Bacterial Activity ◽  
Inhibitory Zone ◽  
Solanum Lycopersicum L ◽  
Mean Diameter ◽  
The Mean

This research aims to know the effectiveness of lycopene antibacterial, through knowing bending zone lycopene towards salmonella thypi. This research has been success to isolate lycopene through extraction process used reflux method at 60oC temperature, using chloroform and methanol as antisolvent. Lycopene extract got 5,12 mg/100 g, analysis of functional groups by using spectroscopy Fourier Transform InfraRed (FTIR) detected C=C alkena alifatik at 1674,91 dan 1639,65 cm-1 wave length, C-H(CH3) detected at 1378,71 cm-1       wavelength, C-H  (stretching)  alifatikfunctional groups detected at 2853,12 cm-1 wave length, C-H alkena (stretching) detected at  2924,16 cm-1 wave length, and C-H alkena (bending) detected at  1498,86  cm-1 wave length. Inhibitory zone testing of salmonella thypi used paperdisc method with three repetitions. The mean diameter of the inhibitory zone of bacteria formed in positive control was 33,17 mm, negative control was 0 mm. Mean diameter of bacterial inhibitory zone at 3 % concentration with 3, 68 mm mean diameter and maximal Inhibitory zone at 50 % concentration with        15,12 mm mean diameter . The result of this research shown that lycopene extract has bacterial activity towards salmonella thypi Kewords: Isolation, Lycopene,  Salmonella thypi, Antibacterial

scholarly journals Ekstrak Umbi Rumput Teki (Cyperus Rotundus) Sebagai Antibakteri Terhadap Staphylococcus Epidermidis Dan Propionibacterium Acnes

2018 ◽  
Vol 9 (2) ◽  
pp. 165-175
Author(s):  
Siti Nurjanah ◽  
Akhmad Rokiban ◽  
Erdy Irawan
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Positive Control ◽  
Negative Control ◽  
Cyperus Rotundus ◽  
Mean Diameter ◽  
Inhibition Zones ◽  
The Mean

The tuber of cyperus rotundus are used to tradisional medicine. C. rotundus known to contain compounds of secondary metabolites such as alkoloids, sineol, pinene, siperon, rotunal, siperenon, siperol, tannins, flavonoids and scientifically it has been believed to have antimicrobial activity. Empirically this plant are used as an antibacterial, antitumor, anticancer, and antiallergic. The purpose of this study were determining the inhibition of extract of Cyperus rotundus against tubers of Staphylococcus epidermidis and Propionibacterium acnes. In this study C. rotundus extracted by maceration using 70% ethanol. The extracts tested against S. epidermidis and P. acnes using disc blank method. The results obtained from the mean diameter of the inhibition zones of exstract at concentrations of 20%, 40%, 60%, 80%, and 100% on S. Epidermidis 8.03 mm, 11.43 mm, 15.04 mm, 15.83 mm and 19.98 mm respectively and P. acnes 11.59 mm, 13.35 mm, 17.17 mm, 20.44 mm, and 30.08 mm respectively Aquades used as negative control and erythromycin used as positive control. Testing were continued by determining the Minimum Inhibitory Concentration (MIC). The result of MIC for S. epidermidis was 15% and P. acnes was 11%. The analysis showed that the antibacterial activity of the tubers extract is bacteriostatic for S. epdermidis and bacterisid for P. acnes.

scholarly journals The difference of inhibitory zone between Katuk (Sauropus androgynous L. Merr.) leaf infusion and Roselle (Hibiscus sabdariffa L.) petals towards oral Candida albicans

2017 ◽  
Vol 29 (2) ◽  
Author(s):  
Kadek Seruni Kusumanegara ◽  
Emma Rachmawati ◽  
Ame Suciati Setiawan
Keyword(s):  
Candida Albicans ◽  
Positive Control ◽  
Negative Control ◽  
Hibiscus Sabdariffa ◽  
Inhibitory Zone ◽  
Experimental Laboratory ◽  
The Mean ◽  
The Difference ◽  
Oral Candida ◽  
Made In

Introduction: Katuk (Sauropus androgynus L. Merr.) leaf and roselle (Hibiscus sabdariffa L.) are part of plants that have antifungal activity against Candida albicans. The purpose of this study was to examine the inhibitory zone of katuk leaf’s infusion compared to roselle petals towards the growth of oral Candida albicans. Methods: The research methods was experimental laboratory. Each of katuk leaf and roselle’s infusion were respectively made in four concentration, i.e 5, 10, 20, and 40%. The positive control was 2% ketoconazole, while negative control was sterile aquadest. Results: The result of the study showed that the mean of inhibitory zones of katuk leaf 5%, was 17.90±0.8 mm, katuk leaf of 10% was 19.67±1.78 mm, katuk leaf of 20% was 19.67±1.78 mm, and katuk leaf consentration of 40% was 22.93±1.00 mm, meanwhile the mean of inhibitions zones of roselle petals infusion of 5% shows its mean of 18.53±0.67 mm, roselle petals of 10% was 22.40±1.28 mm, roselle petals of 20% was 26.20±0.87 mm, and roselle petals of 40%was 29.47±2.87 mm. Conclusion: It can be concluded that the mean of inhibitions zones of katuk leaf’s infusion was smaller than roselle petals toward the growth of oral Candida albicans.

scholarly journals Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255533
Author(s):  
Victor Angelo Martins Montalli ◽  
Patrícia Rejane de Freitas ◽  
Milenna de Figueiredo Torres ◽  
Oscar de Figueiredo Torres Junior ◽  
Dienne Hellen Moutinho De Vilhena ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
High Rate ◽  
Positive Control Group ◽  
Control Group ◽  
Healthy Environment ◽  
The Mean ◽  
Petri Dishes ◽  
Uv C

Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x108 CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm2 of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment.

scholarly journals Application of Scaevola taccada (Gaertn.) Roxb. Reduce Pro-inflammatory Cytokines Interleukin-1β in Sprague Dawley Mice Suffering from Mastitis

2020 ◽  
Vol 8 (A) ◽  
pp. 423-427
Author(s):  
Prihantono Prihantono ◽  
Salman Ardi Syamsu ◽  
Nilam Smaradhania ◽  
Mardiana Ahmad ◽  
Nurul Aini Siagian ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Mean Value ◽  
Negative Control ◽  
Interleukin 1Β ◽  
Sprague Dawley ◽  
Leaf Extracts ◽  
Group Iii ◽  
Group I ◽  
The Mean

BACKGROUND: Mastitis is an inflammation of the breast tissue, usually caused by bacteria. Mastitis stimulates pro-inflammatory cytokines. The cytokine interleukin-1β (IL-1β) is a crucial mediator of the inflammatory response. This cytokine has adverse effects of hosting immunity that mediates resistance to pathogens and also exacerbates damage during chronic disease and acute tissue injury. Scaevola taccada (Gaertn.) Roxb. has been used as an ethnomedicine for healing sores in several provinces in Indonesia. AIM: This study aimed to assess the profile of pro-inflammatory cytokine IL-1β through the treatment effect of leaf extracts of S. taccada (Gaertn.) Roxb. as adjuvant for healing mastitis. METHODS: This study was a true control group experiment using the pre-test-post-test control design aimed to measure the effect of hydroalcoholic compounds in leaf extracts of S. taccada on the systemic pro-inflammatory activity of interleukin-1β (IL-1β). The treated animals were 18 mice of Sprague Dawley strain induced by Staphylococcus aureus. These treated mice were divided into three groups in which each group consisted of six mice. The mice in the Group I (negative control) were given 1 ml aquabides/250 g body weight, those in the Group II (positive control) were delivered with 9.6 mg/ml amoxicillin/250 g body weight, and those in the Group III (experimental) were given 9.6 mg amoxicillin/250 g body weight + 400 mg/ml leaf extracts of S. taccada/g body weight for 5 days, respectively. Pathological examinations were carried out from the inflamed tissues to prove the healing process of the treated mice. IL-1Β levels were determined using the enzyme-linked immunosorbent assay method. Data were analyzed using ANOVA and post hoc tests. RESULTS: There were statistically significant differences of IL-1β levels after the administration of leaf extracts of S. taccada among all the treated mice groups at p < 0.05. The Group III had the lowest IL-1β level with the mean value ± 1.45 pg/ml compared to the IL-1β level in the Group II (positive control) with the mean value ± 3.82 pg/ml and the IL-1β level in the Group I (negative control) with the with mean value ± 5.22 pg/ml. The pathological analysis of breast tissues of the treated mice proved that leaf extracts of S. taccada (Gaertn Roxb.) could reduce damaged tissues, cellular infiltration, and subcutaneous edema induced by this pathogenic microorganism. CONCLUSION: Leaf extracts of S. taccada had a significant function as adjuvant for healing mastitis by reducing pro-inflammatory cytokine IL-1β.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals Biological Effect of Leaf Aqueous Extract ofCaesalpinia pyramidalisin Goats Naturally Infected with Gastrointestinal Nematodes

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Roberto Robson Borges-dos-Santos ◽  
Jorge A. López ◽  
Luciano C. Santos ◽  
Farouk Zacharias ◽  
Jorge Maurício David ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Anthelmintic Activity ◽  
Experimental Period ◽  
Gastrointestinal Nematodes ◽  
Positive Control ◽  
Negative Control ◽  
Immunological Parameters ◽  
Fecal Egg Count ◽  
Leaf Aqueous Extract ◽  
The Mean

Forty-eight goats naturally infected with gastrointestinal nematodes were randomly divided into four groups (n=12): negative control (G1) (untreated), positive control (G2) (treated with doramectin, 1 mL/50 Kg b.w.), and G3 and G4 treated with 2.5 and 5 mg/Kg b.w. of a leaf aqueous extract ofCaesalpinia pyramidalis(CP). Fecal and blood samples were regularly collected for the evaluation of fecal egg count (FEC), hematological and immunological parameters to assess the anthelmintic activity. In treated animals with CP, there was noted a significant reduction of 54.6 and 71.2% in the mean FEC (P<0.05). An increase in IgA levels was observed in G3 and G4 (P<0.05), during the experimental period, suggesting that it was stimulated by the extract administration. In conclusion, the results showed that CP provoked a protective response in infected animals treated with them. This response could be partly explained by the CP chemical composition.

scholarly journals 2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nada Sarah Syahputri ◽  
Nuzul Asmilia ◽  
Rinidar Rinidar ◽  
Amalia Sutriana ◽  
Fakhrurrazi Fakhrurrazi ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Positive Control ◽  
Negative Control ◽  
Hexane Extract ◽  
Phyllanthus Emblica ◽  
Treatment Groups ◽  
Significant Difference ◽  
Treatment Data ◽  
The Mean

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo

scholarly journals Potensi Antibakteri Ekstrak Daging Buah Kelubi (Eleiodoxa conferta) Bangka Belitung Menggunakan Microwave-Assisted Extraction (MAE)

2020 ◽  
Vol 7 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Surtina Surtina ◽  
Ratih Puspita Sari ◽  
Zulita Zulita ◽  
Rani Rani ◽  
Occa Roanisca ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Microwave Assisted Extraction ◽  
Test Results ◽  
Inhibitory Zone ◽  
E Coli ◽  
Microwave Assisted ◽  
Assisted Extraction

Antibacterial tests were carried out using ethanol extract of kelubi meat (Eleidoxa conferta), extraction using the Microwave-Assisted Extraction method with a ratio of 2: 20 for 30 minutes at 60 °C. Antibacterial tests using diffusion methods with concentrations of 20%, 40%, 60%, 80%, and 100%; positive control of 0.005% amoxillin and DMSO negative control. Antibacterial test results showed that the inhibition zone of ethanol extract in E. coli bacteria with a concentration of 20% was in the moderate category, for a concentration of 40% including the strong category while the concentration of 60%, 80% and 100% was very strong. The inhibition zone of ethanol extract for the S. aureus bacterium with a concentration of 20% is included in the strong category while the concentration of 40%, 60%, 80%, and 100% is categorized as very strong. From these results it can be concluded that the inhibitory zone in Stapylococcus aureus bacteria is greater than that of E. coli bacteria.

scholarly journals PENGARUH PEMBERIAN JUS BUAH BELIMBING WULUH (AVERRHOA BILIMBI L.) TERHADAP KADAR KOLESTEROL LDL DARAH TIKUS PUTIH (RATTUS NORVEGICUS) JANTAN GALUR WISTAR DENGAN DIET TINGGI LEMAK

2019 ◽  
Vol 8 (2) ◽  
pp. 35-41
Author(s):  
Kartika Dwi Rahminiwati ◽  
◽  
IGM Antara Hambarsika ◽  
Fitri Handajani ◽  
◽  
...  
Keyword(s):  
Cholesterol Level ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
High Fat ◽  
Cholesterol Levels ◽  
The Mean

A high-fat diet can increase lipoprotein levels, total cholesterol, (Low Density Lipoprotein) LDL, and triglycerides. Starfruit has saponin and flavonoid compounds which are expected to reducing LDL cholesterol levels. The aim of the study was to determine the effect of starfruit juice on lowering the blood cholesterol LDL of Wistar rats fed with high-fat diet. Experimental study with post-test only control group design. As many as 24 male white rats from the Wistar strain were divided into 3 groups: negative control groups (K-) that were given standard feed for 28 days; positive control group (K+) who were given a high-fat diet for 28 days; the treatment group (KP) was given a high-fat diet for 28 days and on the 15th day 28th they were given a starfruit juice with a dose of 4ml / 200grBB / day. Day 29 measured LDL cholesterol. The statistic test showed a significant increase in LDL cholesterol levels (p=0.001) in the positive control group (x=12.125±2.642 mg/dL) compared to the negative control group (x=7.625±1.506 mg/dL). There was no significant different the mean cholesterol level of the treatment group (x=11±1.927) compared to the mean LDL cholesterol level of the positive control group (x=12.125±2.642). A high-fat diet significantly increases the mean LDL cholesterol level. Starfruit juice did not significantly reduce LDL cholesterol level.

scholarly journals THROMBOLYTIC ACTIVITY OF C.AMADA AND C.CAESIA

2017 ◽  
Vol 10 (2) ◽  
pp. 317
Author(s):  
Vijaya Bharathi Saravana Sankar ◽  
Anuradha V ◽  
Ali Ahmad ◽  
Sani Mohammed Tajo
Keyword(s):  
Silver Nanoparticle ◽  
Active Component ◽  
Positive Control ◽  
Negative Control ◽  
Clot Lysis ◽  
P Value ◽  
Thrombolytic Activity ◽  
The Mean ◽  
Control Water

Objective: To compare the thrombolytic activity of C.amada and C. caesia.Methods: Hydro alcoholic extractions of C.amada and C. caesia were prepared. Clot lysis potential of the extracts was checked and the results were statistically analysed.Result and Conclusion: On comparing the thrombolytic potential, the % of clot lysis of silver nanoparticle synthesized using C. caesia (51%) was found to be higher than C.amada (34.7%) extract. Similarly C. caesia extract (38.75 ± 2.217) showed higher % of clot lysis than C.amada (34.74 ± 6.074). The mean percentage of clot lysis of rhizome extracts and synthesized silver nanoparticle were statistically more significant (p value < 0.05) when compared to the positive control streptokinase and negative control water. Active component involvement analysis will be helpful to utilize the C.amada and C. caesia as potent therapeutic agent.Key words: C.amada, C. caesia, thrombolytic potential.

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