scholarly journals Omega-3 Oil Ameliorate Histological and Ultrastructural Alterations Induced by Cadmium Chloride in Rats Testis

2020 ◽  
Vol 4 (1) ◽  
pp. 62-69
Author(s):  
Treefa F. Ismail ◽  
Falah M. Aziz
Keyword(s):  
Drinking Water ◽  
Tap Water ◽  
Occupational Exposures ◽  
Testicular Tissue ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Omega 3 ◽  
Potential Threat ◽  
Ultrastructural Alterations

Cadmium (Cd) is considered to be one of the major environmental pollutants which have potential threat to human health. Reports of declining male fertility have renewed interest in the role of environmental and occupational exposures in the etiology of human infertility. Cd exposure led to obvious degenerative changes in testicular tissue. This study was performed to investigate the Cd-induced structural effects on the testes and to evaluate the possible protective effect of omega-3 oil in adult albino rats. Thirty adult male rats were used in the present work, divided randomly into five groups, six rats in each group; the first group was considered as a control group and left without treatment except the standard rat chow and tap water. The second group was given 40 mg/L of CdCl2 in drinking water while the third group was given 60 mg/L of CdCl2 in drinking water. The fourth group was given 40 mg/L of CdCl2 in drinking water plus omega-3 oil (4 g/kg diet), while the fifth group was given 60 mg/L of CdCl2 in drinking water plus omega-3 oil (4 g/kg diet), the Cd-treated rats showed dose-dependent histological and ultrastructural alterations which have been ameliorated after exposure to omega-3 oil. The present investigation concluded that omega-3 played a protective role against Cd-induced histopathological changes in rat testis.

scholarly journals Biochemical and Haematological Study in Rats Exposed to Cadmium Chloride in Drinking Water

2007 ◽  
Vol 31 (2) ◽  
pp. 15-29
Author(s):  
Hanaa A. A.
Keyword(s):  
Drinking Water ◽  
Blood Cells ◽  
Cadmium Chloride ◽  
Tap Water ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Treated Group ◽  
Physiological Effect ◽  
Male Rats ◽  
Control Group

The objective of this study was investigate the Physiological effect ofdifferent concentrations of cadmium chloride in drinking water on somebiochemical and haematological parameters of male rats. Animals in thisexperiment were randomly divided into four equal groups and treated for 15weeks as follows : Rats in control group were offered ordinary tap water ,while animal in M1 , M2 , and M3 were received 10 , 20 , 30 ppm Cdcl2 indrinking water, respectively. The activity of Alanine aminotransferase(ALP), Aspartate aminotrasferase (AST) and alkaline phosphatase (ALP) inserum were measured. Further mor, hemoglobin concentration (Hb) andwhite blood cells count (WBCs) were detected. The result revealed thataddition of cadmium chloride in different concentrations in drinking watercaused a significant increase in activity of serum of ALT in treated group(M1 , M2 , M3 ) as compound with control. Within the time the activity ofserum AST was significantly increased in three treated group as comparedwith pretreatment period. On the other hand , significant increase in serumALP concentration were show in both M1 and M3 treated groups at 12th and15th week of the treatment as compared with control group. Significantdecrease in haemoglobin concentration were observed in M1 , M2 , M3 from9th week to the end of experiment comparing to control. While cadmiumchloride treatment caused significant increase in total white blood cells countin treated groups as compared with control group. On Conclusion, it seemslikely that exposure of male rats to cadmium chloride at level above thepermissive one had induced clear biochemical and haematological changes.

scholarly journals The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats

2021 ◽  
Vol 25 (1) ◽  
pp. 154-172
Author(s):  
K. K. Khudiar ◽  
B. N. Abdullah ◽  
K. A. Al-Mzaien
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Protective Effect ◽  
Aqueous Extract ◽  
Plasma Cholesterol ◽  
Male Rats ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Albino Rats ◽  
Group I

In this study, the potential protective effect of aqueous extract of parsley (Petroselinum sativum) seeds against hydrogen peroxide (H2O2) – induced oxidative stress in male rats was assessed. Three groups of male albino rats were randomly divided (n=7) and were handled for twenty-eight days as follows: rats in group I served as control; animals in group || were provided with drinking water containing 0.5% H2O2 and those in group III received orally 8 mg/100 gm B.W. of aqueous extract of parsley seeds plus 0.5% H2O2 in drinking water. After four weeks experimental period, a significant increase in lipid peroxidation products (MDA), and decrease in glutathione (GSH) concentrations were observed in plasma, kidney, liver and heart tissues of H2O2 treated animals as compared with the control group. These biomarkers (GSH and MDA) are interrelated and indicate the occurrence of oxidative stress. Plasma total cholesterol (TC) concentration was significantly increased in H2O2 treated rats. By administration of aqueous extract of parsley along with H2O2, plasma and tissue GSH levels were significantly increased while the elevation in MDA level was diminished in plasma and different tissues examined. A decrease in plasma cholesterol concentration was recorded in H2O2 and parsley treated group as compared with the control one and H2O2 treated groups. These results indicate that aqueous extract of parsley have hypocholesterolemic and antioxidant effect.

scholarly journals Study the Effects of Formaldehyde and Xylene Vapor on Lung and Testicular Tissue with Sperm Morphology of Adult Albino Rats

2021 ◽  
Vol 11 (1) ◽  
pp. 46-51
Author(s):  
Trefa F. Ismail ◽  
Galawezh O. Othman ◽  
Naznaz H. Othman ◽  
Ban A. Hassan
Keyword(s):  
Testicular Tissue ◽  
Chemical Substance ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Cytotoxic Effects ◽  
Histological Techniques ◽  
Group A ◽  
Group B

Formalin is widely used chemical substance in industry and medicine (as tissue fixative and disinfectant). It contains reactive molecules which have been known for its cytotoxic effects. Furthermore, xylene used in histological techniques during preparation of tissue and has different toxic effects on tissues. The present study was performed to investigate the effects of formaldehyde and xylene inhalation on albino rat’s lung and testes. Fifteen adult male rats were used in this study; the rats were divided randomly into three groups (A, B, and C). Group (A) comprises the control group, whereas the rats in the Group (B) were exposed to 10% formaldehyde vapor and the rats from Group (C) were exposed to xylene vapor for 4 h/day for 5 days/week for 1 month. Histological alterations were observed in the lung of formalin and xylene exposed rats manifested by lymphatic infiltration, emphysema, congestion, dilation of blood vessels, and alveolar sac and also thinning in the wall of alveolar bronchiole. On the other hand, formaldehyde and xylene affected the testes tissue of treated rats including atrophy in the seminiferous tubule cells, edematous connective tissue, degenerative of spermatogenesis, depletion in germinal layer, existing of vacuoles in the seminiferous tubules, and decrease in the number of sperms in the lumen of seminiferous tubules. Furthermore, sperms abnormalities were seen. The data of our study concluded that inhalation the vapor of formaldehyde and xylene has degenerative effects on lungs and testes of the experimental rats as well as affecting on the spermatocytes and increased the frequency of sperm abnormalities.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

scholarly journals Curcumin Along With Fe3O4 Nanoparticles Improved Sperm Parameters In Rats With Testicular Ischemia

2021 ◽  
Vol 10 ◽  
pp. e2034
Author(s):  
Shabnam Zarei Moradi ◽  
Seyed Abdolhamid Angaji ◽  
Mitra Salehi ◽  
Mehrdad Hashemi
Keyword(s):  
Fe3o4 Nanoparticles ◽  
Sperm Concentration ◽  
Concomitant Administration ◽  
Testicular Tissue ◽  
Sperm Parameters ◽  
Male Rats ◽  
Semen Parameters ◽  
Control Group ◽  
Fe3o4 Nps ◽  
Testicular Ischemia

Background: Ischemic/reperfusion (I/R) in testicular tissue is one reason for the worldwide increase in male infertility. In the present study, we assessed the effects of curcumin and Fe3O4 nanoparticles (NPs) on sperm parameters in rats with I/R damage. Materials and Methods: Forty-eight adult male rats were divided into two groups (n=24 per group): control and torsion/detorsion. The control and torsion/detorsion groups were divided into four subgroups include sham, Fe3O4 NPs, curcumin, and Fe3O4 NPs+curcumin. After the rats were sacrificed, semen was collected from their epididymal tissues to assess sperm viability, motility, concentration, and morphology. Results: Curcumin significantly improved viability, motility, and normal sperm morphology in rats with I/R damage compared to the control group; however, it did not have a significant effect on sperm concentration (P<0.001). Fe3O4 NPs alone decreased all sperm parameters in the control and I/R rats (P<0.001). However, concomitant administration of Fe3O4 nanoparticles with curcumin significantly improved sperm parameters in rats with I/R damage (P<0.001). Conclusion: The increase in all semen parameters in the experimental groups with concomitant use of Fe3O4 NPs plus curcumin indicated that green synthesis of NPs could be recommended for future clinical studies.

scholarly journals Effect of Tramadol on Sperm Profile of Male Albino Rats

2019 ◽  
pp. 1-6
Author(s):  
I. S. Esua ◽  
U. U. Uno ◽  
U. B. Ekaluo
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Head ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Dependent Manner ◽  
Sperm Viability ◽  
Albino Rat ◽  
Significant Difference

Background and Aim: Tramadol is a potent analgesic effective in the treatment of mild to severe pains. However, the use of the drug can pose a threat to other organs and systems. Therefore, this study evaluated the effect of graded doses of tramadol on sperm profile of male albino rats. Materials and Methods: Eighteen male rats were divided into three groups (A, B and C) using completely randomized design (CRD) with six rats in each group. Rats in group A served as the control group and were given just food and water while groups B and C were given tramadol at 50 and 100 mg/kg body weight (BW) respectively, daily for the period of 65 days. The treatment was administered via oral gavage and at the end of the treatments, the rats were sacrificed. Immediately after sacrifice, a puncture was made in the epididymis with a sterile pin and examined for semen pH. The epididymes were processed for epididymal sperm motility, viability, count and sperm head abnormality. Results: There was no significant difference in the weight of testes and semen pH. Sperm viability, sperm motility, sperm count and weight of epididymes significantly reduced (p<0.05) in tramadol treated animals when compared with the control. Results also indicated statistically significant (p<0.05) increase in sperm head abnormalities in rats treated with tramadol when compared with the control. Conclusion: The results obtained from this study reveal that tramadol has negative effects on weight of epididymes, sperm count, sperm viability, sperm motility and sperm head abnormalities in male albino rat as mammalian models in a dose dependent manner.

scholarly journals Effect of Testosterone on Lead Acetate Toxicity in Male Albino Rats

2017 ◽  
Vol 2 (2) ◽  
pp. 112-120
Author(s):  
Nazar Mohammed Shareef Mahmood ◽  
Sarkawt Hamad Ameen Hamad ◽  
Dlshad Hussein Hassan ◽  
Karwan Ismael Othman
Keyword(s):  
Lead Acetate ◽  
Toxic Substance ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Cell Degeneration ◽  
Group I ◽  
Liver And Kidney ◽  
Adverse Influence

The toxicity of lead acetate (L. A.) concerned to public health disruptor due to its persistence in the environment and it has the adverse influence on the human and animal health as well. It causes physiological,biochemical, and neurological dysfunctions in humans. Histologically it has a negative effect on the liver which is considered one of the major target organs where acts as detoxification machine by elimination the toxic substance from the blood in rich with it.  As well as it affects kidneys that are the two of the most filtering organs. Therefore the present study was aimed to investigate the histopathological effect of L.A. on liver and kidney tissues in male rats. Twenty male rats involved in the study were equally and randomly divided into two groups each of them involved 10 animals. Group I (castrated rats) and Group II (control) each group received 80mg/L of lead acetate dissolved in one liter distilled water by drinking for 15 days. Histological sections showed some alterations including abnormal architecture, cell degeneration, nuclear degeneration, hyperchromatic hepatocytes, immune cells, degeneration in tubules, dilation in sinusoids, dilation in central vein of liver increased bowman's space glomerular atrophy degeneration of tubular cells in liver and kidney tissues of rats in castrated rats from control group. But the size of degenerated tissue was more severe in castrated male rats. It was concluded that the castration process could produce a hypogonadism and decreased testosterone which owns many receptors in kidney and liver may produce adverse influence with L.A. administration.

scholarly journals Fructose feeding and intermittent hypoxia affect ventilatory responsiveness to hypoxia and hypercapnia in rats

2004 ◽  
Vol 97 (4) ◽  
pp. 1387-1394 ◽  
Author(s):  
Evelyn H. Schlenker ◽  
Yijiang Shi ◽  
Joni Wipf ◽  
Douglas S. Martin ◽  
Curtis K. Kost
Keyword(s):  
Intermittent Hypoxia ◽  
Plasma Insulin ◽  
Tap Water ◽  
Minute Ventilation ◽  
Acute Hypoxia ◽  
Male Rats ◽  
Control Group ◽  
Insulin Levels ◽  
Ventilatory Responses ◽  
Plasma Insulin Levels

We hypothesized that, in male rats, 10% fructose in drinking water would depress ventilatory responsiveness to acute hypoxia (10% O2 in N2) and hypercapnia (5% CO2 in O2) that would be depressed further by exposure to intermittent hypoxia. Minute ventilation (V̇e) in air and in response to acute hypoxia and hypercapnia was evaluated in 10 rats before fructose feeding (FF), during 6 wk of FF, and after FF was removed for 2 wk. During FF, five rats were exposed to intermittent air and five to intermittent hypoxia for 13 days. Six rats given tap water acted as control and were exposed to intermittent air and subsequently intermittent hypoxia. In FF rats, plasma insulin levels increased threefold in the rats exposed to intermittent hypoxia and during washout returned to levels observed in rats exposed to intermittent air. During FF, ventilatory responsiveness to acute hypoxia was depressed because of decreased tidal volume (Vt) responsiveness. During washout, V̇e decreased as a result of decreased Vt and frequency of breathing, and the ventilatory responsiveness to hypoxia in intermittent hypoxia rats did not recover. In all rats, the ventilatory responses to hypercapnia were decreased during FF and recovered after washout because of an increased Vt responsiveness. In the control group, hypoxic responsiveness was not depressed after intermittent hypoxia and was augmented after washout. Thus FF attenuated the ventilatory responsiveness of conscious rats to hypoxia and hypercapnia. Intermittent hypoxia interacted with FF to increase insulin levels and depress ventilatory responses to acute hypoxia that remained depressed during washout.

scholarly journals Nanoparticles of zinc oxide defeat chlorpyrifos-induced immunotoxic effects and histopathological alterations

2019 ◽  
Vol 12 (3) ◽  
pp. 440-448 ◽  
Author(s):  
Sara S. Essa ◽  
Eiman M. El-Saied ◽  
Osama S. El-Tawil ◽  
Inas M. Gamal ◽  
Sahar S. Abd El-Rahman
Keyword(s):  
Zinc Oxide ◽  
Drinking Water ◽  
Interleukin 2 ◽  
Potential Effect ◽  
Male Rats ◽  
Control Group ◽  
Organophosphate Insecticide ◽  
Zno Nps ◽  
Macrophage Activity ◽  
Serum Lysozyme

Background and Aim: Chlorpyrifos (CPF) is a widely used organophosphate insecticide. Nanoparticles of zinc oxide (ZnO NPs) physically showed effective adsorbing property for some insecticides. The study was conducted to estimate the potential effect of ZnO NPs against CPF toxicity. Materials and Methods: Four groups of male rats were used; control group and three groups received drinking water contained 75 mg/L CPF, combined 75 mg/L CPF and 200 mg/L ZnO NPs, and 200 mg/L ZnO NPs, respectively. Results: CPF significantly decreased macrophage activity, serum lysozyme activity, and levels of interleukin-2 (IL-2) and IL-6; increased the percentage of DNA degeneration on comet assay of lymphocytes and significantly elevated hepatic and splenic malondialdehyde contents; and decreased their glutathione contents. The liver and spleen showed marked histological alterations after exposure to CPF with decreased expression of acetylcholinesterase. The coadministration of ZnO NPs ameliorated most of the undesirable effects of CPF, through elevation of macrophage and serum lysozyme activities, increased the levels of IL-2 and IL-6, corrected the oxidative stress markers, and alleviated most of the adverse effect exerted by CPF in liver and spleen tissues. Conclusion: The addition of ZnO NPs to CPF-contaminated drinking water may be useful as a powerful antioxidant agent against toxic damage induced by CPF particularly in individuals who are on daily occupational exposure to low doses of CPF.

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