scholarly journals Effect of Testosterone on Lead Acetate Toxicity in Male Albino Rats

2017 ◽  
Vol 2 (2) ◽  
pp. 112-120
Author(s):  
Nazar Mohammed Shareef Mahmood ◽  
Sarkawt Hamad Ameen Hamad ◽  
Dlshad Hussein Hassan ◽  
Karwan Ismael Othman
Keyword(s):  
Lead Acetate ◽  
Toxic Substance ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Cell Degeneration ◽  
Group I ◽  
Liver And Kidney ◽  
Adverse Influence

The toxicity of lead acetate (L. A.) concerned to public health disruptor due to its persistence in the environment and it has the adverse influence on the human and animal health as well. It causes physiological,biochemical, and neurological dysfunctions in humans. Histologically it has a negative effect on the liver which is considered one of the major target organs where acts as detoxification machine by elimination the toxic substance from the blood in rich with it.  As well as it affects kidneys that are the two of the most filtering organs. Therefore the present study was aimed to investigate the histopathological effect of L.A. on liver and kidney tissues in male rats. Twenty male rats involved in the study were equally and randomly divided into two groups each of them involved 10 animals. Group I (castrated rats) and Group II (control) each group received 80mg/L of lead acetate dissolved in one liter distilled water by drinking for 15 days. Histological sections showed some alterations including abnormal architecture, cell degeneration, nuclear degeneration, hyperchromatic hepatocytes, immune cells, degeneration in tubules, dilation in sinusoids, dilation in central vein of liver increased bowman's space glomerular atrophy degeneration of tubular cells in liver and kidney tissues of rats in castrated rats from control group. But the size of degenerated tissue was more severe in castrated male rats. It was concluded that the castration process could produce a hypogonadism and decreased testosterone which owns many receptors in kidney and liver may produce adverse influence with L.A. administration.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

scholarly journals PROTECTIVE ROLE AND ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROSMARINUS OFFICINALIS AGAINST TRICHLOROACETATE-INDUCED TOXICITY IN LIVER OF MALE RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 420
Author(s):  
Medhat Mostafa Abozid ◽  
Hoda Ea Farid
Keyword(s):  
Aqueous Extract ◽  
Liver Damage ◽  
Protective Role ◽  
Rosmarinus Officinalis ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Group I ◽  
Glutamyl Transferase

 Objective: The current study was designed to estimate the potential protective role of the aqueous extract of rosemary (AER) (Rosmarinus officinalis) against trichloroacetic acid (TCA)-created hepatotoxicity in male albino rats.Methods: Forty male albino rats were separated into four groups of ten: Group I served as control; Group II was given AER (200 mg/kg/day) by gavage; Group III received TCA at the dose 50 mg/kg/day, and Group V was treated with AER (200 mg/kg/day) and received TCA (50 mg/kg/day). The experiment was carried out for 2 months.Results: The toxicity of TCA for rats was revealed by an elevation in liver marker enzymes activities (gamma-glutamyl transferase [GGT], alkaline phosphatase [ALP], aspartate transaminase [AST], alanine aminotransferase [ALT]) and conjugated bilirubin (CB) level, and a decrease in albumin and total protein (TP) levels. The TCA administration also caused a significant increase in the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and also malondialdehyde (MDA) level in liver tissues. These biochemical effects were accompanied by histological indicators of liver damage. Treatment with ARE recovered the liver damage instigated by TCA, as showed by perfection of liver enzyme markers (GGT, ALT, AST, ALP), CB, TP and albumin; as well as antioxidant parameters (CAT, SOD, GPx) and lipid peroxidation (MDA) and amelioration of histopathology changes in the liver tissues.Conclusion: It could be concluded that AER supplementation for 2 months in TCA-induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA.

Protective effect of N-acetylcysteine, caffeic acid and vitamin E on doxorubicin hepatotoxicity

2007 ◽  
Vol 26 (6) ◽  
pp. 519-525 ◽  
Author(s):  
A. Gokcimen ◽  
A. Cim ◽  
H.T. Tola ◽  
D. Bayram ◽  
A. Kocak ◽  
...  
Keyword(s):  
Vitamin E ◽  
Caffeic Acid ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Protective Effects ◽  
Injection Group ◽  
Hepatocellular Damage ◽  
Group I ◽  
Significant Difference

The aim of this study was to compare the possible protective effects of N-acetylcysteine (NAC), caffeic acid (CAPE) and vitamin E (Vit-E) on doxorubicin-induced hepatotoxicity. Thirty-two male Wistar albino rats, weighing between 250 and 350 g were supplied and randomly divided into five groups. Animals in study groups were pretreated with a single dose of doxorubicin (Dox), which was administered intraperitoneally (i.p.). Control group (Group I) was treated with intraperitoneal saline injection. Group II did not received any antioxidant agent after the injection. Group III and Group IV were given CAPE and intraperitoneal vitamin E injection for eight days, respectively. Group V received NAC for eight days. The study was finished after 10 days. Tissue samples were collected from all animals and histopathological examination was performed. There was statistically significant difference between the experiment groups and controls by means of mononuclear cell infiltration and diameters of hepatic sinusoid, terminal hepatic venule (central vein) and portal area (portal canal). Changes related with hepatocellular damage were more prominent, whereas there was no significant difference between Dox and NAC given groups histopathologically. It was observed that structural changes were regressed after CAPE administration. However, this recovery was more prominent in vitamin E given group. These findings suggest that Dox induced liver damage could be efficiently reversed by vitamin E administration. It has been found that CAPE, but not NAC has protective effects on Dox-induced hepatocellular damage. Human & Experimental Toxicology (2007) 26, 519—525

scholarly journals The effect of broccoli (Brassica oelracea var botrytis) extract to the microanatomy structure of liver and kidney in mice (Mus musculus L.) after exposed by lead acetate (Pb-acetate) orally

2006 ◽  
Vol 4 (1) ◽  
pp. 14-21
Author(s):  
RIRIN DIYAH SETYANINGSIH ◽  
NOOR SOESANTI HANDAJANI ◽  
MARTI HARINI
Keyword(s):  
Mus Musculus ◽  
Lead Acetate ◽  
Negative Control Group ◽  
Negative Control ◽  
Proximal Tubules ◽  
Control Group ◽  
Average Weight ◽  
Central Vein ◽  
Damage Level ◽  
Liver And Kidney

The aims of this research were to know the effect of broccoli extract to the microanatomy structure of liver and kidney in mice after induction of lead acetate. Broccoli contains antitoxic compound such as sulforaphane, beta-carotene, indole, quercetin, and glutathione. The research was evaluated experimentally to Swiss mice at the age of 2 months with average weight of 31 g. These mice were divided into five groups. A group was a placebo controls that given only with distilled water 1 ml for 30 days. Negative control group received lead acetate orally at the dose of 0.52 mg/g body weights for 15 days. Mice of group 1, 2, and 3 received the same treatment but continued with giving broccoli extract orally in three doses: 2.1 mg; 2.8 mg and 3.5 mg/g bw/day respectively for 15 days. Liver and kidney were removed at 31st day. Observation of microanatomy structure was: 1) Determining the kind and damage level of hepatocyte, glomerulus and proximal tubules epithelia as the qualitative data; 2) Measuring the height of proximal tubules epithelia, glomerulus diameter and central vein diameter as the quantitative data, which then analyzed with ANOVA. The result of this research showed that exposed by lead acetate orally caused hydrophilic degeneration, fatty swelling and necrotic (pycnotic, karyorrhexis, and karyolysis) on hepatocyte and proximal tubules epithelia, swelling of glomeruli. Consumption of broccoli at the dose of 3.5 mg/g bw/day could repair the damage cells of lead acetate. Broccoli extract at the lowest dose (2.1 mg/g bw/day and 2.8 mg/g bw/day) could not repair the damage cells of lead acetate. Consumption of broccoli extract at the dose of 3.5 mg/g bw/day was helping the cells repairmen (i.e. hepatocyte, glomerulus and proximal tubule epithelia) in mice (Mus musculus. L) damage by lead acetate.

Cytoprotective Effects of Zingiber officinale Against the Oxidative Stress Induced by Lead Acetate Toxicity in Rats

2020 ◽  
Author(s):  
O. Aouacheri ◽  
S. Saka
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Lead Acetate ◽  
Zingiber Officinale ◽  
Treated Group ◽  
Normal Diet ◽  
Male Rats ◽  
Control Group ◽  
Experimental Diet ◽  
Group I

The evaluation of the effect of ginger on the modulation of toxic effects induced by lead is the objective of our study. Forty male rats were randomly divided into four groups and treated daily for 3 consecutive months. Group I (0-0) was kept as control; group II (0-G) received an experimental diet with 2% of ginger; group III (Pb-0) received 2% lead acetate dissolved in drinking water with a normal diet; and group IV (Pb-G) received 2% lead acetate in drinking water and an experimental diet containing 2% ginger. Lead acetate exposure caused a significant increase of organosomatic indexes, hepatic, lipid, and urine profiles. In addition, lead acetate has a pro-oxidative effect expressed by a significant decrease in tissue GSH levels and the enzymatic activity of GPx and CAT. This pro-oxidative action was also marked by an increase in MDA level and GST activity in lead-treated group. Feeding ginger-supplemented diet to lead acetate-treated rats restored all the parameters studied as compared to control. These results suggest that ginger treatment exerts a protective effect on metabolic disorders by decreasing the oxidative stress.

scholarly journals Amitriptyline Induced Alterations in Liver and Kidney Functions and Structures in Male Rats

2019 ◽  
pp. 1-10
Author(s):  
Fathy A. M. Atta ◽  
Ehab Tousson ◽  
Noha A. Dabour ◽  
Ahmed A. Massoud ◽  
Ahmed F. Hasan
Keyword(s):  
Tissue Injury ◽  
Tricyclic Antidepressant ◽  
Chloride Ions ◽  
Male Rats ◽  
Mental Health Issue ◽  
Control Group ◽  
Albino Rats ◽  
Membrane Pump ◽  
Mitochondrial Functions ◽  
Liver And Kidney

Aims: Depression is a mental health issue that starts most often in early adulthood and it is a common and recurrent disorder causing significant morbidity and mortality worldwide. Amitriptyline is a tricyclic antidepressant that is known to inhibit the presynaptic reuptake of serotonin, norepinephrine, and inhibitor of mitochondrial functions and induces apoptosis in several tissues. This study aims to identify the changes in liver and kidney structure and functions after treatment of male rats with Amitriptyline drugs. Materials and Methods: A total of 20 male albino rats were randomly and equally divided into 2 groups (G1, control group that included animals that did not receive any treatment during the experimental period. G2, Amitriptyline (Tryptizol; El Kahira Pharm And Chem Ind Co) group in which rats were injected intraperitoneally with Amitriptyline (100 mg/kg body weight/daily) for four weeks). Results: The current results revealed that; Amitriptyline treatments significantly (P <0.05) increased the levels of serum ALT, AST, ALP, urea, creatinine, sodium ions, chloride ions and liver  and kidney damages as compared to control. In contrast; a significant (P <0.05) decrease in albumin, and total protein, potassium ions and calcium ions in Amitriptyline group was reported when compared with control group. Conclusion: Amitriptyline has many side effects on rat liver and kidney, it induced liver and kidney toxicity and tissue injury were it metabolized to nortriptyline which inhibits the reuptake of norepinephrine and serotonin almost equally. Amitriptyline inhibits the membrane pump mechanism responsible for uptake of norepinephrine and serotonin in adrenergic and serotonergic neurons.

scholarly journals Aqueous fruit pulp extract of Adansonia digitata (L) protects against lead-acetate-induced hepato-renal damage in rat model

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Wusa Makena ◽  
Eduitem Sunday Otong ◽  
Nathan Isaac Dibal ◽  
Barka Ishaku ◽  
Sebastine Anthony Bazabang
Keyword(s):  
Kidney Function ◽  
Lead Acetate ◽  
Antioxidant Activities ◽  
Liver Steatosis ◽  
Control Group ◽  
Adansonia Digitata ◽  
Fruit Extract ◽  
Group I ◽  
Kidney Glomerulus ◽  
Liver And Kidney

Abstract Background Adansonia digitata (L) fruit has a multi-purpose function one among many, is the antioxidant activities of the fruit by preventing oxidative stress. The effect of Adansonia digitata (L) fruit on lead-induced liver and kidney damage is not clear. Hence, the study was aimed to assessed the protective role of Adansonia digitata (L) fruits against lead acetate induced changes in the liver and kidney function test parameters and the histology of both organ in experimental rats. The rats were divided into five groups with five rats each. All the rats were administered with respective assigned treatment once daily for 6 weeks. Rats in groups I were administered with just distil water (2 ml/kg). Rats in groups II were administered with lead acetate (30 mg/kg) while rats in groups III–V were administered Adansonia digitatata (L) fruit extract (250 mg/kg and 500 mg/kg) and Succimer (5 mg/kg) respectively, then additionally challenged with lead acetate (30 mg/kg) immediately after. At the end of the administration, the blood serum from the experimental rats were used for biochemical analysis. Then, the the organs such as the liver and kidney collected for histological study. Results Rats administered with Lead acetate showed an increase in AST, ALP and ALT as well as increase in urea and creatinine level (p < 0.001), when compared with the control group (group I), where as Adansonia digitatata (L) fruit prevented the effect (upsurge of serum, Urea, Creatinine, AST, ALP and ALT) of lead acetate. Rats administer with only Lead acetate revealed marked liver steatosis and the degeneration of the kidney glomerulus. The Adansonia digitatata (L) fruit extract and Succimer prevented the histological liver steatosis, as well as the degeneration of the glomerulus of the kidney cytoarchitecture. Conclusion The findings in this study suggest that Adansonia digitata fruits extract has a protective potentials against lead acetate induced liver and kidney toxicity by preventing the upsurge of liver function enzymes and kidney function parameters. Hence, Adansonia digitata fruits can serve as a natural plant agent that can prevent hepato-renal toxicity. Therefore, Adansonia digitata holds future prospects in preclinical framework to ameliorate organs toxicity for oral therapeutic applications.

scholarly journals Chemotherapeutic Efficacy of Indigofera aspalathoides on 20-Methylcholanthrene-Induced Fibrosarcoma in Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Selva Kumar Sivagnanam ◽  
Mudiganti Ram Krishna Rao ◽  
Maruthaiveeran Periyasamy Balasubramanian
Keyword(s):  
Sialic Acid ◽  
Aqueous Extract ◽  
Group Iv ◽  
Control Group ◽  
Albino Rats ◽  
Activity Levels ◽  
Aqueous Extracts ◽  
Group Iii ◽  
Group I ◽  
Liver And Kidney

The present study was undertaken to test the chemopreventive effects of one herbal medicinal plant, Indigofera aspalathoides, on chemically induced carcinogenesis in rats. A well-known polyaromatic hydrocarbon, namely, 20-methylcholanthrene, which is a known carcinogenic substance, was used to induce fibrosarcoma in Wistar strain of male albino rats. Fibrosarcoma rats were treated with aqueous extracts of Indigofera aspalathoides. The rats were divided into four groups, each consisting of six animals. Group I served as normal control, Group II served as fibrosarcoma-induced animals, Group III were fibrosarcoma-bearing animals treated with aqueous extracts of Indigofera aspalathoides, and Group IV animals, which were normal healthy animals treated with Indigofera aspalathoides aqueous extract, served as drug control set. Group III and Group IV animals were treated with aqueous extract of Indigofera aspalathoides intraperitoneally at a dose of 250 mg/kg. b.w. for 30 days. The fibrosarcoma was proved by pathological examinations. The activity levels of nucleic acids such as total DNA and RNA and hexose, hexosamine, and sialic acid in liver and kidney of treated rats were used to monitor the chemopreventive role of the plant extract. The observed increase in the levels of DNA, RNA, hexose, hexosamine, and sialic acid in liver and kidney tissues of fibrosarcoma-bearing animals reached near normal state after the treatment with aqueous extracts of Indigofera aspalathoides, suggesting that Indigofera aspalathoides does have a chemotherapeutic role.

scholarly journals Protective Effect of Starch-stabilized Selenium Nanoparticles against Melamine-induced Hepato-renal Toxicity in Male Albino Rats

2021 ◽  
Author(s):  
Zainab Sabry Othman Ahmed ◽  
Mona K. Galal ◽  
Elsayed A. Drweesh ◽  
Khaled S. Abou-El-Sherbini ◽  
Eman A.M. Elzahany ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Renal Toxicity ◽  
Selenium Nanoparticles ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Antioxidant Genes ◽  
Expression Levels ◽  
Group I

Melamine and its analogues are illegally added to raise the apparent protein content in foods. The elevated concentrations of these compounds cause adverse effects in humans and animals. In this contribution, the protective effects of the synthesized starch-stabilized selenium nanoparticles (Se-NPs@starch) on melamine-induced hepato-renal toxicity have been systematically investigated. The Se-NPs@starch were characterized by X-ray photoelectron spectroscopy (XPS) analysis, energy dispersive spectroscopy (EDS) mapping analysis, TEM, and FT-IR. Starch plays a crucial role in the stabilization and dispersion of Se NPs, as noticed from the TEM and EDS investigations. Furthermore, the atomic ratio of Se distribution over the starch surface is approximately 1.67%. The current study was conducted on four groups of adult male rats, and the oral daily treatments for 28 days were as follows: group I served as control, group II received Se-NPs@starch, group III was exposed to melamine, while group IV was treated with melamine and Se-NPs@starch. The results reveal a significant alteration in the histoarchitecture of both hepatic and renal tissues induced by melamine. Furthermore, elevated liver and kidney function markers, high malondialdehyde, and increased expression levels of apoptosis-related genes besides a reduction in GSH and expression levels of antioxidant genes were observed in the melamine-exposed group. Interestingly, the administration of the Se-NPs@starch resulted in remarkable protection of rats against melamine-induced toxicity through increasing the antioxidant capacity and inhibiting oxidative damage. Collectively, this study provides affordable starch-stabilized Se-NPs with potent biological activity, making them auspicious candidates for prospective biomedical applications.

scholarly journals Garlic Alleviates the Injurious Impact of Cyclosporine-A in Male Rats through Modulation of Fibrogenic and Steroidogenic Genes

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Mustafa Shukry ◽  
Saqer S. Alotaibi ◽  
Sarah M. Albogami ◽  
Nora Fathallah ◽  
Foad Farrag ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cyclosporine A ◽  
Transforming Growth Factor ◽  
Substantial Reduction ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
Cell Degeneration

This work aimed to study the hepato-testicular protective effect of garlic in rats treated with cyclosporine A (CsA). Forty male Westar albino rats were randomly distributed in five groups (8 rats each): control, olive oil, garlic, CsA, and CsA co-treated with garlic. CsA induced an upsurge in the alanine transaminase, aspartate transaminase, and alkaline phosphatase levels and decreased albumin and total protein levels, expression of superoxide dismutase (SOD) gene, serum testosterone, triiodothyronine, and thyroxine levels compared to the control group. Additionally, there was an increase in the cholesterol, triglyceride, and low-density lipoprotein levels and a substantial reduction in the high-density lipoprotein levels compared to the control groups. Histopathological investigation of the liver showed abnormalities like hepatic cell degeneration, congestion of blood vessels, and highly active Kupffer cells in the CsA group. Histopathological examination of testes showed damaged seminiferous tubules, stoppage of the maturation of spermatogonia, and the presence of cells with irregular dense nuclei in the lumina of some tubules. For the groups treated with garlic, mitigation of the damage caused by CsA in the liver and testes, liver function tests, lipid profiles, and hormones was seen along with improved gene expression of SOD and steroidogenesis genes, and decreased gene expression of collagen I-α1 and transforming growth factor-1β. Conclusively, garlic had a positive impact on CsA-induced hepatic and sperm toxicity. It is recommended that garlic should be supplemented in transplant treatments using CsA to alleviate the cyclosporin-induced oxidative injuries and other harmful effects.

Sign in / Sign up

Export Citation Format

Share Document