The effect of supportive E. coli mastitis treatment on PMN chemiluminescence and subpopulations of T lymphocytes

2013 ◽  
Vol 16 (4) ◽  
pp. 671-677
Author(s):  
H. Markiewicz ◽  
W. Krumrych ◽  
M. Gehrke
Keyword(s):  
T Lymphocytes ◽  
Control Group ◽  
Polymorphonuclear Leucocytes ◽  
Sample Collection ◽  
Blood Indices ◽  
E Coli ◽  
Flunixin Meglumine ◽  
Acute Form ◽  
The Impact ◽  
Antibiotic Product

Abstract The aim of this field study was to assess the impact of a single i.m. injection of lysozyme dimer and flunixin meglumine in combination with intramammary and systemic antibiotic on chemiluminescence of PMN (polymorphonuclear leucocytes) and subpopulations of lymphocyte T in blood of cows with E. coli mastitis. Examinations were performed on 30 dairy cows affected with naturally occurring acute form of E. coli mastitis. Cows were randomly divided into three groups according to the method of treatment. The first group was treated with approved intramammary antibiotic product, the same antibiotic in i.m. injection and one injection of flunixin meglumine on the first day of therapy. Next group was treated with the same antibiotic and additionally one injection of lysozyme dimer on the first day of therapy. The third one was treated only with an antibiotic and served as a control group. Blood samples were taken before treatment and on days 3 and 7. In samples haematology indices were determined, spontaneous and opsonised zymosan stimulated CL and PMA measurements were performed and the subpopulations of T lymphocyte (CD2+, CD4+, CD8+) were assayed in whole blood. There was no effect of the applied supportive treatment on the value of morphological blood indices. A significant influence of the time of sample collection on the level of CL and dynamics of lymphocytes T subpopulation was demonstrated. A single injection of flunixin meglumine or lysozyme dimer on the day of the beginning of treatment of E. coli mastitis, does not affect the level of neutrophil chemiluminescence and the percentage of T lymphocytes in the blood of mastitic cows in the analysed period of time.

scholarly journals Effect of Whole-Body Cryotherapy on Morphological, Rheological and Biochemical Indices of Blood in People with Multiple Sclerosis

2021 ◽  
Vol 10 (13) ◽  
pp. 2833
Author(s):  
Bartłomiej Ptaszek ◽  
Aneta Teległów ◽  
Justyna Adamiak ◽  
Jacek Głodzik ◽  
Szymon Podsiadło ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Neurological Diseases ◽  
Venous Blood ◽  
Whole Body ◽  
Controlled Study ◽  
Control Group ◽  
Blood Indices ◽  
Rheological Indices ◽  
Whole Body Cryotherapy ◽  
The Impact

The aim of this study was to examine and assess the impact of a series of 20 whole-body cryotherapy (WBC) treatments on the biochemical and rheological indices of blood in people with multiple sclerosis. In this prospective controlled study, the experimental group consisted of 15 women aged 34–55 (mean age, 41.53 ± 6.98 years) with diagnosed multiple sclerosis who underwent a series of whole-body cryotherapy treatments. The first control group consisted of 20 women with diagnosed multiple sclerosis. This group had no intervention in the form of whole-body cryotherapy. The second control group consisted of 15 women aged 30–49 years (mean age, 38.47 ± 6.0 years) without neurological diseases and other chronic diseases who also underwent the whole-body cryotherapy treatment. For the analysis of the blood indices, venous blood was taken twice (first, on the day of initiation of whole-body cryotherapy treatments and, second, after a series of 20 cryotherapy treatments). The blood counts were determined using an ABX MICROS 60 hematological analyzer (USA). The LORCA analyzer (Laser–Optical Rotational Cell Analyzer, RR Mechatronics, the Netherlands) was used to study the aggregation and deformability of erythrocytes. The total protein serum measurement was performed using a Cobas 6000 analyzer, Roche and a Proteinogram-Minicap Sebia analyzer. Fibrinogen determinations were made using a Bio-Ksel, Chrom-7 camera. Statistically significant differences and changes after WBC in the levels of red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), elongation index, total extend of aggregation (AMP), and proteins (including fibrinogen) were observed. However, there was no significant effect of a series of 20 WBC treatments on changes in blood counts, rheology, and biochemistry in women with multiple sclerosis. Our results show that the use of WBC has a positive effect on the rheological properties of the blood of healthy women.

scholarly journals 449 Late-Breaking: Impact of feeding a propriety yeast-based synbiotic product on fecal shedding of top-7 Shiga toxin-producing Escherichia coli in feedlot cattle

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 141-141
Author(s):  
Raghavendra Amachawadi ◽  
Xiaorong Shi ◽  
LeighAnn George ◽  
Miles Theurer ◽  
Twig Marston ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Virulence Genes ◽  
Multivariable Analysis ◽  
Feedlot Cattle ◽  
Control Group ◽  
Fecal Samples ◽  
Fecal Shedding ◽  
E Coli ◽  
Multiplex Pcr Assay ◽  
The Impact

Abstract Shiga toxin-producing E. coli (STEC) belonging to serogroups O26, O45, O111, O103, O121, O145, and O157, called ‘top-7’, are major foodborne pathogens. Cattle are a major reservoir, in which STEC colonize the hindgut and are shed in the feces, which is a major source of contamination of food. Our objective was to evaluate the impact of a proprietary yeast-based synbiotic product (prebiotic and probiotic; Alltech, Inc., Nicholasville, KY) on fecal shedding of top-7 STEC in feedlot cattle. Twenty existing pens, housing 40–112 steers per pen, with an estimated 60 to 90 days to slaughter, were randomly assigned to a control group or a treatment group that received 22 g of the synbiotic product per steer per day, as a top dress, in a finishing diet. Twenty pen-floor fecal samples were collected from each pen on days 0, 21, 42, and 54. Fecal samples were enriched and subjected to a multiplex PCR assay targeting serogroup-specific genes for the top-7 STEC and three major virulence genes, stx1 (Shiga toxin 1), stx2 (Shiga toxin 2), and eae (intimin). Bivariate descriptive statistics for the major serogroups and virulence genes were assessed prior to multivariable analysis using mixed effects logistic regression. The overall prevalence of the top-7 serogroups were 44.5% of O26, 41.3% of O157, 15.1% of O103, 13.7% of O45, 7.8% of O121, and 0.6% of O111. The overall prevalence of stx1, stx2, and eae were 43.9%, 70.8%, and 49%, respectively. E. coli O26, O157, and O45 had a significant treatment and sampling day interaction (P < 0.0001). On d 42, fecal samples from treated group had lower prevalence (P < 0.01) of O26, O103, and O45 compared to the control group. In conclusion, the in-feed administration of the synbiotic product appears to reduce fecal shedding of certain top-7 STEC serogroups in the feedlot cattle.

scholarly journals Effect of whole-body cryotherapy on morphological, rheological, and biochemical blood indices in individuals with multiple sclerosis

2021 ◽  
Author(s):  
Bartłomiej Ptaszek ◽  
Aneta Teległów ◽  
Justyna Adamiak ◽  
Jacek Głodzik ◽  
Szymon Podsiadło ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Positive Impact ◽  
Erythrocyte Aggregation ◽  
Whole Body ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Blood Indices ◽  
Whole Body Cryotherapy ◽  
The Impact

Abstract The study aim was to examine the impact of 20 whole-body cryotherapy sessions on biochemical and rheological blood indices in multiple sclerosis individuals. The study group involved 15 women (mean age: 41.53 ± 6.98 years) with diagnosed multiple sclerosis who underwent whole-body cryotherapy sessions. The first control group consisted of 20 women (mean age: 40.45 ± 4.77 years) with multiple sclerosis who received no cryotherapy intervention. The second control group comprised 15 women (mean age: 38.47 ± 6.0 years) without neurological diseases or other chronic conditions who participated in cryotherapy sessions. For blood indices analysis, venous blood was collected twice: on the day of cryotherapy commencement and after the 20 cryotherapy sessions. Blood counts were determined with a hematology analyzer. A laser-optical rotational cell analyzer served to investigate erythrocyte aggregation and deformability. Total serum protein was measured, and proteinogram and fibrinogen values were established. Statistically significant differences were observed in red blood cells, hemoglobin, hematocrit, elongation index, total extent of aggregation, proteins (including fibrinogen). There was no significant effect of the 20 cryotherapy sessions on morphological, rheological, or biochemical blood indices in women with multiple sclerosis. The intervention had a positive impact on the rheological blood properties of healthy women.

scholarly journals Bacterial contamination of soil and manure on a cattle farm, depending on the period and place of sample collection

2016 ◽  
Vol 72 (12) ◽  
pp. 777-781
Author(s):  
Beata Trawińska ◽  
Bożena Nowakowicz-Dębek ◽  
Anna Chmielowiec-Korzeniowska ◽  
Leszek Tymczyna ◽  
Magdalena Pyrz ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Sample Collection ◽  
Cattle Farm ◽  
E Coli ◽  
Multiple Comparison Test ◽  
Proteolytic Bacteria ◽  
Microclimatic Conditions ◽  
The Impact ◽  
Number Of Bacteria

The aim of bacteriological examinations was to evaluate soil and manure taken from dairy farms at different times and sampling sites. The study was conducted from September to October. Soil samples were collected directly at the wall of the barn (GI) and at distances of 15 m (GII) and 45 m (GIII) from the livestock facilities. Manure was collected inside the building, at the entrance (KI) and at 1/4 (KII) and ½ (KIII) of its length. Air temperature, relative humidity and the moisture content of the samples were recorded at the sampling sites. The soil and manure samples were subjected to quantitative and qualitative bacteriological examinations conducted in accordance with Polish Standards. Statistical analysis of the results was performed by a one-way analysis of variance and Duncan’s multiple comparison test. The highest number of bacteria in the soil was found at the wall of the barn. Most mesophilic bacteria, actinomycetes, coliforms and E. coli were found in late April and early May, most psychrophilic microorganisms in June, and most proteolytic bacteria at the end of December and in early January. The microbiological contamination of the soil was low (coli titer ≤0.01), amounting to 0.001, and it was detected only at the wall of the barn. The qualitative study of soil revealed only E. coli bacteria. The highest number of microbes in manure was found in samples from the middle of the barn (KIII) taken at the end of April and at the beginning May. Out of the bacteria examined in this study, E. coli and Enterobacter spp. were found in manure samples throughout the study period. While analyzing the impact of selected parameters, such as microclimate and humidity, on manure samples tested for bacteria, a correlation was observed between the presence of all microbes and sample moisture. The bacterial contamination of soil and manure was the biggest during the spring period, which was probably due to climatic and microclimatic conditions. However, the contamination was negligible owing to the absence of pathogenic bacteria and a high soil index. This proved that proper zoohygienic conditions were maintained on the farm.

scholarly journals Study of some Impact Of Enterococcus faecium As Probiotic On Chick 1- Body Weight And Pathological Changes In Air Sacs And Intestine

2007 ◽  
Vol 31 (1) ◽  
pp. 70-77
Author(s):  
Emad J. Khamas
Keyword(s):  
Body Weight ◽  
High Temperature ◽  
Enterococcus Faecium ◽  
Control Group ◽  
Pathological Changes ◽  
Fourth Group ◽  
E Coli ◽  
The Third ◽  
Air Sacs ◽  
The Impact

To investigate the impact of Enteococcus faecium as probiotic in layer chicks,this bacteria was given in feed at a dose rate of 1.5 gm per kg feed, each kg offeed contained 5×1011 cfu along the period of the study (57 days). 150 one dayold males were divided into five groups, 30 males each. The 1st group was giventhe probiotic only in the feed, the 2nd group was given the probiotic and exposedto high temperature and bad ventilation as a stress, and the third group wasgiven the probiotic with chlortetracycline in feed while the fourth group wasgiven the probiotic and then infected with E. coli on 21 day of age and the fifthgroup was given feed with out probiotic as the control group. The resultsshowed that there was increase in body weight especially in the last weeks of theexperiment. Turbidity of the air sacs was mild in the four group compared withcontrol. Intestinal villi became elongated due to using of probiotic and there wasless sloughing compared with control, and those groups showed resistancetoward E. coli infection.

scholarly journals Influence of cadmium on the cellular part of the immune system of young cattle

2020 ◽  
Vol 3 (2) ◽  
pp. 47-52
Author(s):  
Y. Y. Lavryshyn ◽  
B. V. Gutyj ◽  
K. Y. Leskiv ◽  
I. I. Hariv ◽  
L. H. Yevtukh ◽  
...  
Keyword(s):  
Immune System ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cadmium Chloride ◽  
Control Group ◽  
T Suppressors ◽  
Wide Range ◽  
Young Cattle ◽  
The Impact ◽  
Experimental Group

The article presents the results of research on the effect of cadmium load on the body of young cattle. The negative effect of Cadmium on the liver causes a wide range of pathological changes at different levels of its organization. The impact of heavy metals on the immune system is particularly significant, because it performs a leading role in maintaining health and is recognized as one of the most sensitive to adverse factors, even in relatively low concentrations. The aim of the study was to investigate the influence of cadmium on the cellular part of the immune system of young cattle. The research was carried out on 10 bulls of six months of age, Ukrainian black-and-white dairy breed, which were formed into 2 groups of 5 animals each: control and experimental. The bulls of the control group were on a normal diet. Animals of the experimental group were administered cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. It was found that feeding bulls with a diet of cadmium chloride, the number of B-lymphocytes on the 5th day of the experiment was 17.54 ± 0.95 %. The lowest number of B-lymphocytes was on the 20th day of testing – 15.12 ± 0.37 %. The study of the number of T-lymphocytes shows that at the beginning of analysis the number of T-lymphocytes in the blood of animals of the control and experimental groups ranged from 40.70 ± 3.62 and 40.85 ± 2.54 %. Subsequently, the number of T-lymphocytes in the blood of the experimental group began to decline. The lowest number of T-lymphocytes was in the experimental group of animals on the 20th day of the research, compared with the control group, this figure decreased by 3.63 %. The state of immunity of animals under cadmium load significantly depends on the ratio of T-helpers to T-suppressors. It was found that the immunoregulatory index of blood of bulls of the experimental group probably decreased from 10 days of the research. The number of T-helper lymphocytes in the blood of the experimental group of bulls under cadmium loading is probably reduced by 15, 20 and 30 days of testing. A probable increase in the number of T-suppressors was noted in the bull's blood of the experimental group on the 20th day of the research. Immunoglobulins of different classes are crucial among bull’s serum proteins under cadmium loading. The concentration of immunoglobulins in the blood under cadmium load decreased by 15.9 % relative to the control group on the 20th day of analysis.

scholarly journals HIV infection and Mycoplasma co-infection: case-control study in a female population of Douala (Cameroon)

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Essome Henri ◽  
Nida Martine Colette ◽  
Mve Koh Valère ◽  
Ekono Michel Roger ◽  
Nana Njamen Théophile ◽  
...  
Keyword(s):  
Ureaplasma Urealyticum ◽  
Case Control ◽  
Cd4 Count ◽  
Control Group ◽  
Case Group ◽  
Sample Collection ◽  
Female Population ◽  
Biological Variables ◽  
Hiv Women ◽  
The Impact

Objective: To determine the impact of Human Immuno-Deficiency Virus (HIV) on co-infection with Mycoplasma hominis and Ureaplasma urealyticum in HIV-infected women. Methodology: A case-control analytical study based on standardized questionnaire interview and cervical sample collection after informed consent obtained among HIV-positive and HIV-negative women from January 2nd, 2017 to June 30th, 2017 received at the laboratory of Laquintinie Hospital in Douala. Samples collected were used for mycoplasma research, quantification, and antibiogram using the mycoplasma IES kits. Socio-demographic, clinical and biological variables of interest were entered and analyzed on Microsoft Office Excel 2013 and Statistical Package for Social Sciences (SSPS) software version 20 and the chi-square correlation test was used with significance at the threshold of P <5%. Results: We analysed 136 samples of women aged 18-65years, among which 96 HIV+(cases) matched with 40 HIV-(controls). The mean age of the case group was 39.08±10.22 years and that of the control group was 33.28±8.68 years. Genital mycoplasmas were found in 58.3% of cases, with a high frequency for U. urealyticum (19.8%) against 2.1% for M. hominis and the two germs were associated in 32.3% of cases. In the control group, the carrier rate was 62.5%, with a frequency of 17.5% for Ureaplasma alone and no carrier for M. hominis alone. Co-infection with the two germs in this group was 40%. The majority of HIV+women had a CD4 count above 200, and no significant association was found between CD4 count and the presence of mycoplasma in these women (P=0.094). Conclusion: Mycoplasma infection is common in HIV+ women. However, there is no significant association between the CD4 count and the presence of these mycoplasmas.

Effects of Zinc Combined with Probiotics on Antibiotic-associated Diarrhea Secondary to Childhood Pneumonia

2018 ◽  
Vol 65 (5) ◽  
pp. 421-426
Author(s):  
Rong Xiang ◽  
Qing Tang ◽  
Xiu-Qi Chen ◽  
Mu-Yan Li ◽  
Mei-Xiong Yang ◽  
...  
Keyword(s):  
Combined Treatment ◽  
Intestinal Flora ◽  
Serum Levels ◽  
Control Group ◽  
E Coli ◽  
Probiotic Treatment ◽  
Antibiotic Associated Diarrhea ◽  
Before And After ◽  
Lactate Levels ◽  
The Impact

AbstractAimThe aim of this study was to evaluate the impact of zinc combined with probiotics (Bifico) on antibiotic-associated diarrhea (AAD) secondary to pneumonia.MethodsA total of 50 patients with AAD secondary to pneumonia were randomly divided into a probiotics group (Bifico) and a combined group (zinc combined with Bifico) and 25 pneumonia patients without AAD as the control group. Serum levels of zinc, diamine oxidase (DAO) activity, D-lactate and intestinal flora [Bifidobacterium, Escherichia coli and Bifidobacterium/E. coli (B/E) ratio] were detected before and after intervention.ResultsThe results showed that zinc combined with Bifico had significantly higher overall efficiency than Bifico alone for treatment of AAD secondary to pneumonia. Notably, the combined treatment increased the population of Bifidobacterium, while the number of E. coli was reduced, the B/E value was improved and DAO activity and D-lactate levels were markedly reduced.ConclusionPatients with AAD secondary to pneumonia benefit from zinc supplementation of probiotic treatment.

scholarly journals Effects of In-Feed Chlortetracycline Prophylaxis in Beef Cattle on Animal Health and Antimicrobial-Resistant Escherichia coli

2016 ◽  
Vol 82 (24) ◽  
pp. 7197-7204 ◽  
Author(s):  
Getahun E. Agga ◽  
John W. Schmidt ◽  
Terrance M. Arthur
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Human Health ◽  
Bacterial Infections ◽  
Animal Health ◽  
Agricultural Practices ◽  
Control Group ◽  
Content Type ◽  
E Coli ◽  
The Impact

ABSTRACTConcerns have been raised that in-feed chlortetracycline (CTC) may increase antimicrobial resistance (AMR), specifically tetracycline-resistant (TETr)Escherichia coliand third-generation cephalosporin-resistant (3GCr)E. coli. We evaluated the impact of a 5-day in-feed CTC prophylaxis on animal health, TETrE. coli, and 3GCrE. coli. A control group of cattle (n= 150) received no CTC, while a CTC group (n= 150) received in-feed CTC (10 mg/lb of body weight/day) from the 5th to the 9th day after feedlot arrival. Over 25% (38/150) of the animals in the control group developed illnesses requiring therapeutic treatment with antimicrobials critically important to human medicine. Only two animals (1.3%) in the CTC group required such treatments. Fecal swab and pen surface occurrences of genericE. coli(isolated on media that did not contain antimicrobials of interest and were not isolated based on any specific resistance), TETrE. coli, and 3GCrE. coliwere determined on five sampling occasions: arrival at the feedlot, 5 days posttreatment (5 dpt), 27 dpt, 75 dpt, and 117 dpt. On 5 dpt, TETrE. coliconcentrations were higher for the CTC group than the control group (P< 0.01). On 27 dpt, 75 dpt, and 117 dpt, TETrE. coliconcentrations did not differ between groups. 3GCrE. colioccurrences did not differ between control and CTC groups on any sampling occasion. For both groups, generic, TETr, and 3GCrE. colioccurrences were highest on 75 dpt and 117 dpt, suggesting that factors other than in-feed CTC contributed more significantly to antimicrobial-resistantE. colioccurrence.IMPORTANCEThe occurrence of human bacterial infections resistant to antimicrobial therapy has been increasing. It has been postulated that antimicrobial resistance was inevitable, but the life span of the antimicrobial era has been prematurely compromised due to the misuse of antimicrobials in clinical and agricultural practices. Direct evidence relating the use of antimicrobials in livestock production to diminished human health outcomes due to antimicrobial resistance is lacking, and the U.S. Food and Drug Administration has taken an approach to maximize therapeutic efficacy and minimize the selection of resistant microorganisms through judicious use of antimicrobials. This study demonstrated that prophylactic in-feed treatment of chlortetracycline administered for 5 days to calves entering feedlots is judicious, as this therapy reduced animal morbidity, reduced the use of antimicrobials more critical to human health, and had no long-term impact on the occurrence of antimicrobial-resistantE. coli.

scholarly journals Effect of competitive exclusion in rabbits using an autochthonous probiotic

2017 ◽  
Vol 25 (2) ◽  
pp. 123 ◽  
Author(s):  
Sara Cunha ◽  
Ângelo Mendes ◽  
Dinis Rego ◽  
Diana Meireles ◽  
Ruben Fernandes ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Competitive Exclusion ◽  
Alternative Methods ◽  
Pulse Field ◽  
Control Group ◽  
Feed Conversion ◽  
Faecal Microbiota ◽  
E Coli ◽  
Faecal Samples ◽  
The Impact

<p>Animal nutrition has been severely challenged by the ban on antimicrobials as growth promoters. This has fostered the study of alternative methods to avoid colonisation by pathogenic bacteria as well as to improve the growth of animals and feed conversion efficiency. These new options should not alter the normal intestinal microbiota, or affect it as little as possible. The use of probiotics, which are live microorganisms that beneficially affect the host by improving its intestinal microbial balance, can be seen as a promising way to achieve that goal. In this study, New Zealand White rabbits were fed diets containing an autochthonous probiotic of Enterococcus spp., with the strains EaI, EfaI and EfaD, and Escherichia coli, with the strains ECI 1, ECI 2 and ECD, during a 25-d trial, to evaluate the impact of the probiotic on the faecal microbiota, including population dynamics and antimicrobial resistance profiles. A control group of rabbits, which was fed a diet containing a commonly used mixture of antimicrobials (colistin, oxytetracycline, and valnemulin), was also studied. To assess the colonisation ability of the mentioned probiotic, the faecal microbiota of the rabbits was characterised up to 10 d after the administration had ended. Isolates of enterococci and E. coli were studied for phylogenetic relationships using enterobacterial repetitive intergenic consensus (ERIC-PCR) and pulse-field gel electrophoresis (PFGE), respectively. Although partially affected by an unexpected clinical impairment suffered by the rabbits in the experimental group, our results showed the following. The difference between the growth rate of the animals treated with antimicrobials and those fed the probiotic was not statistically significant (P&gt; 0.05). The competitive exclusion product was present in the faecal samples in a large proportion, but stopped being recovered by culture as soon as the administration ended and the housing conditions were changed. Multidrug-resistant strains of enterococci and E. coli were more commonly recovered from faecal samples of animals fed diets containing antimicrobials, than from rabbits fed diets with our probiotic formula. The use of E. coli probiotics to prevent infection by enteropathogenic strains must be carefully considered due to the possible occurrence of gastrointestinal signs. On the other hand, enterococci strains may be more effective, but lack the long-term colonisation ability.</p>

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