scholarly journals Antibacterial potency from the waste of durian rind (Durio zibethinus Murr.) against Propionibacterium acnes that causing acnes

2019 ◽  
Vol 23 (1) ◽  
pp. 8
Author(s):  
Made Mira Pratiwi ◽  
Retno Kawuri ◽  
I Putu Gede Ardhana
Keyword(s):  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Extract ◽  
Banana Peel ◽  
Fruit Peel ◽  
Bacteria Growth ◽  
Durio Zibethinus ◽  
Compound Type ◽  
Inhibitory Power ◽  
Durian Rind

One of the things that could cause pathogenesis of acne is the activity of normal flora bacteria on the skin, one of them is the bacteria Propionibacterium acnes. The use of extracts which is derived from severalparts of plant such as banana peel, mangosteen peel, dragon fruit peel, potato peel, crinum lily leaf, Marsh fleabane leaf, soursop leaf, soma leaf, green betle vine leaf, and cacao seed that are known to have antibacterial activity could help in acne healing attempt. This research was conducted at Microbiology Laboratory Faculty of Mathematics and Natural Sciences Udayana University from December 2017 until March 2018.The research was conducted with the intention toknow the appropriate solvent to obtain the compound type of durian rind extract dissolved with three types of solvent (methanol, ethanol, ethyl acetate), to look for the smallest resistor value (MIC) of the extract with a solvent that provides the best inhibitory power, and to know the compound type of the extract with a solvent that provides the best inhibitory power. The method is diffusion wells and phytochemical tests. The data that is obtained in the study were analysed by analysis of variance (ANOVA). In inhibition test against P. acnes, it is known that the durian rind ethyl acetate extract effectively showed the inhibitory effect on the bacteria growth, with MIC value of 1.1%. The compound that is contained in the durian rind ethyl acetate extract is terpenoid, steroid, flavonoid, phenolic, and tannin.

scholarly journals Antibacterial Activity of Red yeast rice Extract against Propionibacterium acnes ATCC 11827 and Methicilin-Resistant Staphylococcus aureus ATCC BAA-1683

2021 ◽  
Vol 6 (2) ◽  
pp. 83
Author(s):  
Tiana Milanda ◽  
Ade Zuhrotun ◽  
Ulya Nabila ◽  
Vesara A. Gathera ◽  
Arif S.W Kusuma
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Extract ◽  
Acne Vulgaris ◽  
Resistance Testing ◽  
Red Yeast Rice ◽  
Skin Infections ◽  
Red Yeast

Propionibacterium acnes ATCC 11827 and Methicillin-Resistant Staphylococcus aureus ATCC BAA-1683 are bacteria that cause skin infections, namely acne vulgaris and skin and soft tissue infection/SSTI. The increase in the number of resistant bacterial strains, such as MRSA,  requires the search for alternative antibiotics, including using natural ingredients. Red yeast rice is a product of rice fermentation by Monascus purpureus, which is known to have antibacterial, antifungal, antitumor, antioxidant, anti-inflammatory, anti-cholesterol and immunomodulator. This study aims to determine the antibacterial activity of several red yeast rice extracts against these bacteria that cause skin infections. The research was carried out through the stages of collecting materials and identifying the yeast isolates, extracting of red yeast rice, phytochemical screening of red yeast rice extract, confirmation of bacterial test, preparation of bacterial test suspension, testing for bacterial resistance, testing for antibacterial activity of red yeast rice extract and determining the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the most active extract. The results showed that the ethyl acetate and ethanol extracts had antibacterial activity against P. acnes ATCC 11827 and MRSA ATCC BAA-1683. Both extracts contain compounds from the polyphenols, flavonoids, quinones and saponins group. Ethyl acetate extract was the most active extract with MIC values against P. acnes ATCC 1182 and MRSA ATCC BAA-1683 of 50 mg/mL and 12.5 mg/mL. The MBC values of ethyl acetate extract against these bacterial tests were 100 mg/mL and 25 mg/mL. The ethyl acetate extract is more active against MRSA ATCC BAA-1683 than against P. acnes ATCC 11827. From the results of this study it is known that red yeast rice has activity against bacteria that cause skin infections, especially against MRSA

scholarly journals Optimum Conditions for Extraction of Antibacterial Compounds from Citrus Aurantifolia Fruit Peel Waste

2018 ◽  
Vol 14 (1) ◽  
pp. 34-39
Author(s):  
Rima Munawaroh
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Citrus Fruit ◽  
Optimum Conditions ◽  
Fruit Peel ◽  
Fresh Sample ◽  
Citrus Aurantifolia ◽  
E Coli ◽  
Antibacterial Assay ◽  
Fruit Processing

Citrus fruit peel is a major waste in citrus fruit processing industry. The research on extraction activecompounds of Citrus aurantifolia (lime) fruit peel waste and antibacterial activity assay has been done. Theaim of research was to get optimum condition to extract their active compounds which have antibacterialactivity. The dried lime fruit peel was extracted by maceration method using ethanol 48%, 72%, and 96%.The dried and fresh lime fruit peel were also extracted using ethyl acetate. Antibacterial assay was done bydiffusion agar against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The resultshowed that optimal condition to extract antibacterial compound using fresh sample with ethyl acetate assolvent. The ethyl acetate extract of fresh sample was more active against S. aureus than E. coli.

scholarly journals Identifikasi Senyawa Kimia Fenolik Dalam Ekstrak Etil Asetat Kulit Buah Jengkol (Archidendron pauciflorum (Benth.) I.C Nielsen)

2019 ◽  
Vol 11 (02) ◽  
pp. 111-119
Author(s):  
Ahmad Sopian Pian
Keyword(s):  
Mass Spectrometry ◽  
Ethyl Acetate ◽  
Reduction Method ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Processed Food ◽  
Fruit Peel ◽  
Fruit Skin ◽  
Radical Reduction

Jengkol (Archidendron pauciflorum (Benth.) I.C Nielsen) is a plant that is already familiar inIndonesia and is widely used as a processed food that is quite popular. One of the underutilized parts ofthe jengkol plant is the skin. Jengkol fruit peel contains alkaloid compounds, flavonoids, tannins,glycosides, sapoinin and steroids or triterpenoids. The purpose of this study was to determine the contentof chemical compounds in ethyl acetate extract of jengkol fruit peel, and to test antioxidant activity byDPPH free radical reduction method. The results of isolation, purification in ethyl acetate extract ofjengkol fruit skin does not provide activity as an antioxidant. The results of the analysis using FTIRspectrophotometry, Gas chromatography-Mass Spectrometry (GC-MS) and Nuclear Magnetic ResonanceSpectroscopy ( 1 HNMR).

scholarly journals In Vitro Antibacterial and Anti-Inflammatory Effects of Novel Insect Fungus Polycephalomyces phaothaiensis Extract and Its Constituents against Propionibacterium acnes

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 274 ◽  
Author(s):  
Witsanu Sonyot ◽  
Supaporn Lamlertthon ◽  
Janet Jennifer Luangsa-ard ◽  
Suchada Mongkolsamrit ◽  
Kanchana Usuwanthim ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Inflammatory Cytokines ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Extract ◽  
Acne Vulgaris ◽  
Chemical Study ◽  
Culture Broth ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

Propionibacterium acnes plays an important role in the pathophysiology of acne vulgaris, the most common chronic inflammatory skin disease of the pilosebaceous unit. This study was conducted to investigate whether the entomopathogenic fungus Polycephalomyces phaothaiensis components have antibacterial and anti-inflammatory effects against P. acnes that may serve for acne treatment. A chemical study by spectroscopic analysis resulted in the identification of seven known compounds. The anti-P. acnes potency of extracts and test compounds was determined by both agar diffusion and broth dilution methods. The ethyl acetate extract from culture broth along with cordytropolone (1) and stipitalide (2) exhibited strong anti- P. acnes activity while (+)-piliformic acid (3) showed weak inhibitory activity. The anti-inflammatory effect of ethyl acetate extract and 1–3 was then examined by the quantification of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α on heat-killed P. acnes induced cytokine production by THP-1 cells. The result demonstrated that the extract and its constituents (1–3) showed a potent significant effect by inhibiting the P. acnes-induced pro-inflammatory cytokines production in THP-1. Our results suggest for the first time that P. phaothaiensis and its constituents (1 and 2) hold therapeutic value for further studies as a new alternative treatment for acne.

scholarly journals ANTIBACTERIAL ACTIVITIES OF BELIMBING WULUH (AVERRHOA BILIMBI L.) ETHYL ACETATE EXTRACT ON GEL FORMULATED AGAINST PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS

2020 ◽  
pp. 224-228
Author(s):  
DEWI PERTIWI ◽  
IHSANUL HAFIZ ◽  
WARDATUL JANNAH ◽  
HANAFIS SASTRA WINATA ◽  
MELIA SARI ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Extract ◽  
Skin Irritation ◽  
Diffusion Method ◽  
Skin Bacteria ◽  
Dispersion Test ◽  
And Staphylococcus Aureus

Objective: Averrhoa bilimbi is a plant that is used as a food spice and traditional medicine in Indonesia. The purpose of this study was to obtain a gel formula and measure its activity in inhibiting the growth of skin bacteria, namely Propionibacterium acnes and Staphylococcus aureus. Methods: The gel formulation was tested organoleptically, homogeneity, pH, dispersibility test, and skin irritation test. Antibacterial activity test using agar diffusion method, inhibition of bacterial growth was compared with clindamycin as a positive control. Results: All gel formulations showed good homogeneity, pH of the preparations ranged from 4.5 to 6.1, the dispersion test was 5.5-6.5 cm, and none of them caused irritation to the skin. The results of the inhibitory activity against the growth of Propionibacterium acnes bacteria on concentrations 10, 15, and 20% were 16.67 + 0.40, 22.70 + 0.32 and 28.10 + 0.36 and Staphylococcus aureus bacteria were 18.53 + 0.22, 24.16 + 0.29 and 30.40 + 0.4.  The inhibitory activity of clindamycin against Propionibacterium acnes and Staphylococcus aureus bacteria were 35.33+0.29 and 36.30+0.37. Conclusion: Ethyl acetate extract gel Averrhoa bilimbi had good activity in inhibiting the growth of Propionibacterium acnes and Staphylococcus aureus bacteria and has the potential to be used as an herbal anti-acne drug.

scholarly journals Khasiat dan Profil Kromatogram Fraksi Aktif dari Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) yang Diiradiasi

2017 ◽  
Vol 15 (2) ◽  
pp. 160
Author(s):  
Ermin Katrin ◽  
Setiananda Jacobs ◽  
Hendig Winarno
Keyword(s):  
Ethyl Acetate ◽  
Gamma Irradiation ◽  
Irradiation Dose ◽  
Ethyl Acetate Extract ◽  
Leukemia Cells ◽  
Heating Process ◽  
Cytotoxicity Test ◽  
L1210 Leukemia ◽  
Fruit Peel ◽  
Garcinia Mangostana

The mangosteen fruit peel (Garcinia mangostana L.) are used as anti-infl ammatory, antihistamine, treatment of heart disease, antibacterial, antifungal, it is also used for the treatment or therapy of cancer, because it has a cytotoxic activity against cancer cells. Most of Indonesian people have used the mangosteen fruit peel and they produce mangosteen peel into powder or herbal medicine industry has produced its extract in capsules. Efforts to preserve dried mangosteen rind has done by heating process or by gamma irradiation technique. This study aimed to study the effect of gamma irradiation on cytotoxicity activity of extracts and active fractions mangosteen fruit peel against L1210 leukemia cells and chromatogram profi le of extracts and active fractions of the mangosteen fruit peel. Dry powder mangosteen rind irradiated using gamma irradiation dose of 5; 7.5; 10; and 15 kGy. Then each sample was successive macerated in n-hexane, ethyl acetate, and ethanol. Each extract was tested cytotoxicity against L1210 leukemia cells. The ethyl acetate extract was most active extract (IC50 = 4.17 mg/mL) compared with n-hexane extract (IC50 = 8.29 mg/mL) and ethanol extract (IC50 = 7.52 mg/mL). Fractionation of ethyl acetate extract by column chromatography were obtained 6 fractions. The result of cytotoxicity test showed that fraction 1 was the most active fraction (IC50 = 3.97 mg/mL), it was it was still categorized as potential anticancer (IC50 ≤ 20 mg/mL). Profi le chromatogram of fraction 1 with TLC-densitometry showed patches of discoloration on irradiation dose of 10 and 15 kGy. The results of analysis by HPLC fraction 1 showed a decrease of peak area at a dose of 10 kGy was signifi cantly different from the control. Based on the chromatogram profi le of fraction 1 and itscytotoxicity against L1210 leukemia cells, so the maximum dose of 7.5 kGy gamma irradiation can  beap plied on irradiation of mangosteen fruit peel without changing its effi cacy as anti-cancer agent.

scholarly journals Antibacterial Activity of Flavonoids from Ethyl Acetate Extract of Milk Banana Peel (Musa x paradisiaca L.)

2021 ◽  
Vol 28 (3) ◽  
pp. 223
Author(s):  
Wiwik Susanah Rita ◽  
Ida Ayu Raka Astiti Asih ◽  
I Made Dira Swantara ◽  
Ni Luh Yuli Damayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Banana Peel ◽  
Active Fraction ◽  
Active Compounds

Infectious diseases caused by Staphylococcus aureus and Escherichia coli can be treated by milk banana peels (Musa × paradisiaca L.). This study aims to reveal  the  antibacterial  activity  of  milk  banana  peel  ethyl  acetate  extract against Staphylococcus aureus and Escherichia coli, and to identify their active compounds.  Extraction  was  conducted  by  maceration  at  room  temperature, followed by partition. Separation was carried out by column chromatography with mobile phase of chloroform: ethyl acetate (7.5:2.5) and stationary phase of  silica gel  60.  Antibacterial assay  was  performed by  well  diffusion method and  identification  of  active  compounds  was  analyzed  by UV-Vis spectrophotometry and LC-MS/MS. Extraction of the peel powder produced methanol extract,  partition of the methanol extract resulted n-hexane, ethyl acetate, n-butanol, and water fractions. Antibacterial activity assay shows that ethyl acetate extract was the most active to inhibit the growth of both bacteria. Separation with column chromatography resulted 5 fractions. Identification of the  most active  fraction  with UV-Vis showed that the isolate gave maximum absorption at λ 339.00 nm (band  I) and λ 262.00  nm (band  II) which were thought to be flavonol (3-OH substituted). Analysis  with LC-MS/MS shows that the most active fraction contained 3-methacrylate flavonols and 3-(furan- 2yl) acrylate flavonols.

Antinociceptive profile of ethyl acetate extract of Hyptis fruticosa Salzm. ex Benth. (Labiatae)

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
RM Marçal ◽  
DM Ptak ◽  
RR Krempser ◽  
MR Krempser ◽  
SV Floresta ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

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