The Participation of the Organism Adaptation Mechanisms to the Lack of Oxygen According to the Assessment of the Fethemoglobin Content in the Peripheral Blood of Patients with Community-acquired Pneumonia

Introduction. Сommunity-acquired pneumonia (COP) is a global socio-medical problem. At emergence of pneumonia by any genesis, hypoxia develops. Oxygen homeostasis of the body is provided by the coordinated interaction of external respiration, circulatory system and oxygen-transport system of the blood. Hypoxia, due to the malfunction of the external respiratory system, causes the formation of compensatory changes, in the implementation of which involved components of the oxygen transport system. Molecular genetic mechanisms play an important role in the body's adaptation to oxygen deficiency. Fetal hemoglobin (FetHb), having an increased affinity for oxygen, makes a significant contribution to the body's adaptation to new conditions with altered gaseous environment in the presence of pathological processes occurring with hypoxia. In this regard, it is interest to determine FetHb in adults with COP to study its effect on the diagnosis, prognosis and outcome of the disease. The aim of the study. To determinate the participation of the organism adaptation mechanisms to the lack of oxygen according to the assessment of the content of fetal hemoglobin in the peripheral blood of patients with community-acquired pneumonia. Materials and methods. We examined 34 adult patients (18 women and 16 men) with COP, aged 18 to 80 years, who were in the therapeutic department of the City Clinical Hospital № 25 in Kharkiv. The control group was formed of 20 healthy individuals. Spirography was performed on the diagnostic complex "Valenta"; hematological examinations – on the analyzer "ADVIA 60"; measurement of pO2 and pCO2, oxygen saturation, content of fetal hemoglobin – on the device "RAPIDLAB865". Results. In patients with community-acquired pneumonia, there was a decrease of the ventilatory function of external respiration, which is confirmed by a marked decrease in partial oxygen pressure. Oxygen saturation of blood was reduced in the group of patients with COP, but the difference was not statistically significant 94.8 ± 1.0 %. This indicates the presence of compensatory mechanisms aimed at maintaining adequate blood oxygen saturation. Significant increase in pH (from 7.40 to 7.53) and decrease in standard bicarbonate (from 1.27 to 0.68 mmol/l) resulting from violation of the gas composition of the blood can be regarded as a manifestation of partially compensated respiratory alkalosis. In patients with COP, there was a reduction in the total time of hemolysis, a shift of the maximum erythrogram to the left and an increase in the maximum itself, indicating a sharp decline in erythrocyte resistance. The proportion of erythrocytes with reduced resistance was twice as large as similar forms in the control group and the number of highly resistant cells in patients with COP sharply decreased. Obviously, oxygen starvation-mediated stress erythropoiesis is accompanied by the entry into the circulation of functionally defective erythrocytes. They are subject to accelerated elimination from the vascular bed, which causes a decrease in the quantitative indicators of red blood (erythrocyte content, hemoglobin) while maintaining corpuscular parameters (Mean Corpuscular Volume, Mean Cell Hemoglobin Concentration). At the same time, the analysis of individual hemoglobin fractions revealed an increase in the proportion of fetal hemoglobin (from 2.90 ± 0.31 % in the group of healthy individuals to 5.43 ± 1.05 % in patients with COP) (p less than 0.05). Conclusions. Changes in the parameters of acid hemolysis, fetal hemoglobin in the peripheral blood of patients with community-acquired pneumonia with impaired pulmonary ventilation function indicate their participation in the mechanisms of adaptation to oxygen deficiency and they have informative potential. Elevated fetal hemoglobin in peripheral blood in these patients can be used as an indicator of hypoxia, accompanied by impaired oxygen delivery to tissues, which should be used as an additional criterion for diagnosing tissue hypoxia and justify the timely appointment of antihypoxia drugs. Keywords: hypoxia, community-acquired pneumonia, red blood cells, fetal hemoglobin.

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Circulating Natural IgM Antibodies against Angiogenin in the Peripheral Blood Sera of Patients with Osteosarcoma as Candidate Biomarkers and Reporters of Tumorigenesis

Biomarkers in Cancer ◽

10.4137/bic.s6040 ◽

2010 ◽

Vol 2 ◽

pp. BIC.S6040 ◽

Cited By ~ 4

Author(s):

Yulia A. Savitskaya ◽

Genaro Rico ◽

Luis Linares ◽

Roberto González ◽

René Téllez ◽

...

Keyword(s):

Early Diagnosis ◽

Sensitivity And Specificity ◽

Peripheral Blood ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Individuals ◽

Igm Antibodies ◽

Potential Biomarker ◽

Increased Risk ◽

Natural Igm

Background Tumor immunology research has led to the identification of a number of tumor-associated self antigens, suggesting that most tumors trigger an immunogenic response, as is the case in osteosarcoma, where the detection of natural serum IgM antibodies might achieve the diagnosis of osteosarcoma. Natural IgM antibodies to tumor-associated proteins may expand the number of available tumor biomarkers for osteosarcoma and may be used together in a serum profile to enhance test sensitivity and specificity. Natural IgM antibodies can be consistently detected in the peripheral blood sera months to years before the tumor is diagnosed clinically. The study of the level of a potential biomarker many months (or years) prior to diagnosis is fundamentally important. Integrated circulating and imaging markers in clinical practice treating osteosarcoma have potential applications for controlling tumor angiogenesis. Objectives To study the expression of natural IgM antibodies to the tumor antigens of angiogenesis in the peripheral blood sera of osteosarcoma patients and healthy individuals, and to develop serum-based predictive biomarkers. Methods Peripheral venous blood samples were collected from 117 osteosarcoma patients and 117 patients with other tumors. All diagnosis was histologically confirmed. Staging of patients was performed according to the Enneking Surgical Staging System. The control group consisted of 117 age- and sex- matched healthy individuals. In this study, novel immunoconjugates were designed, synthesized and then used to develop a rapid, specific and sensitive enzyme-linked immunosorbent assay (ELISA) method to detect angiogenin (ANG)–IgM directly in the peripheral blood sera of humans. Results Serum ANG–IgM levels are significantly higher in osteosarcoma patients than in healthy individuals ( P < 0.005). Serum ANG–IgM levels varied widely, but were highly dependent on the concentration of IgM (r = 0.85; P < 0.0005). We found ANG–IgM in the sera of 85% of newly diagnosed osteosarcoma patients and ANG–IgM levels were significantly higher in osteosarcoma patients compared to any other tumors ( P < 0.001). Conclusions These results demonstrated that the combined biomarker ANG–IgM has greater sensitivity and specificity in early diagnosis of osteosarcoma patients than the traditional biomarkers (ANG and vascular endothelial growth factor). Circulating ANG–IgM immune complexes can potentially serve as a biomarker for increased risk of osteosarcoma, because relatively high serum levels were also detected in otherwise healthy individuals with a first degree family history of osteosarcoma and in patients with a diagnosis of benign conditions. Immunological aspects of angiogenesis for managing osteosarcoma will have a practical value in early diagnosis, prognosis and monitoring response to antiangiogenic therapy.

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CCR5 and RANTES/CCL5 Gene Polymorphisms Affect the Risk of EBV Reactivations after Allogeneic HSCT.

Blood ◽

10.1182/blood.v108.11.1262.1262 ◽

2006 ◽

Vol 108 (11) ◽

pp. 1262-1262

Author(s):

Katarzyna Bogunia-Kubik ◽

Anna Kuzyk ◽

Emilia Jaskula ◽

Andrzej Lange

Keyword(s):

Chemokine Receptor ◽

Peripheral Blood ◽

Blood Cells ◽

Detection Threshold ◽

Deletion Mutation ◽

Viral Reactivation ◽

Control Group ◽

Healthy Individuals ◽

Hematopoietic Stem ◽

Ebv Reactivation

Abstract Epstein-Barr virus (EBV) reactivation is a serious complication affecting the recipients of allogeneic hematopoietic stem cell transplants (HSCT). Chemokines and their receptors play a major role in the inflammatory and immune responses that mediate allograft outcome. CC-chemokine receptor-5 (CCR5) is known to be involved in perpetuation of HIV infection (as a co-receptor for HIV entry) and the clinical course of this infection is favored by the presence of the 32-nucleotide deletion within the CCR5 gene (CCR5Δ32 polymorphism). The CCR5 deletion mutation (CCR5Δ32) results in a non-functional chemokine receptor. In the present study the CCR5Δ32 polymorphism and two single nucleotide substitutions (−28 C/G; −403 G/A) within, one of the CCR5 ligands, the CCL5/RANTES gene were analyzed in 75 HSCT recipients, 75 donors and related with EBV load. The control group constituted 99 healthy individuals. DNA was extracted from peripheral blood taken into EDTA using silica membranes. Viral load were assessed 2-3 moths after transplantation in blood cells employing a real-time PCR technique. The detection threshold for viral reactivation equaled 10 EBV-DNA copies/105 peripheral blood cells. EBV reactivation was detected in 26 patients. The CCR5Δ32 and RANTES (−28 C/G; −403 G/A) polymorphisms were analyzed by PCR and PCR-RFLP technique, respectively. Distributions of CCR5 and RANTES −28 genotypes were similar in patients, donors and healthy individuals. RANTES −403 AA homozygosity was more frequent in donors than controls (0.61 vs 0.47, p=0.036). The higher number of EBV copies was detected in patients lacking CCR5Δ32 deletion (0.92 vs 0.71, p=0.031). No significant correlation between EBV reactivation and RANTES −28 C/G polymorphism. However, patients transplanted with donors homozygous for RANTES −403 AA (genotype associated with an increased expression of the RANTES gene) more frequently presented with EBV reactivation (0.85 vs 0.49, p=0.002). In conclusion, the presence of the CCR5 deletion-mutation in the recipient and RANTES-403 G in the donor of HSC appeared to lower the susceptibility for EBV reactivation.

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Arterial dysfunction and systemic inflammation in patients with bronchial asthma

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja711 ◽

2012 ◽

Vol 9 (1) ◽

pp. 42-49

Author(s):

E A Sobko ◽

A Y Kraposhina ◽

O P Ischenko ◽

I V Demko ◽

A B Salmina ◽

...

Keyword(s):

Asthma Control ◽

Severe Asthma ◽

Bronchial Asthma ◽

Peripheral Blood ◽

Vascular Wall ◽

Control Group ◽

External Respiration ◽

Steroid Dependent ◽

Healthy Donors ◽

And Function

Background. The objective of this study was to estimate a vascular wall status of large arteries and function of endothelium in patients with different clinical forms of bronchial asthma throughout the disease progression. 220 patients with bronchial asthma have been examined, including 106 persons with moderate asthma ( 1 st group), 61 persons with severe asthma (2 nd group), and 53 persons with steroid-dependent asthma. Control group was formed from 40 healthy donors. Methods. We have assessed parameters of external respiration, arterial rigidity, and the levels of TNFα, IL-6, sCD31 (sPECAM-1), CRP in the peripheral blood at the time of exacerbation and 48 weeks later. Results. We found elevation of IL-6 and TNFα levels in all the tested groups in the period of exacerbation comparing to the control group (p

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THE DIAGNOSTIC ROLE OF FETAL HEMOGLOBIN AND BLOOD OXYGEN SATURATION IN CHRONIC LIVER DISEASES

Archiv Euromedica ◽

10.35630/2199-885x/2021/11/4.20 ◽

2021 ◽

Vol 11 (4) ◽

pp. 77-78

Author(s):

Boleslav Levitan ◽

Vsevolod Skvortsov ◽

Тatiana Kasyanova ◽

Maksim Vozniuk

Keyword(s):

Liver Cirrhosis ◽

Chronic Hepatitis ◽

Oxygen Saturation ◽

Liver Diseases ◽

Sodium Dodecyl ◽

Fetal Hemoglobin ◽

Control Group ◽

Blood Oxygen ◽

Blood Oxygen Saturation ◽

Diagnostic Role

This research aims to study levels of fetal hemoglobin and blood oxygen saturation in 264 patients with chronic diffuse liver diseases, among them 69 patients with chronic hepatitis and 195 patients with liver cirrhosis. To determine the levels of fetal hemoglobin, a patented method of rocket electrophoresis in agar gel with sodium dodecyl sulfate was used. The level of fetal hemoglobin in liver cirrhosis were significantly increased compared to chronic hepatitis, and, in both cases, compared with the control group. Oxygen saturation of the blood was reduced mainly in liver cirrhosis. In patients with liver cirrhosis a relationship between fetal hemoglobin and oxygen saturation was established. Our study helps in the early diagnosis of latent chronic hypoxia and hypoxemia in patients with liver disease. This allows to assess disease severity and adjust the treatment

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Clinical significance of the determination of surfactant proteins A and D in assessing the activity of lung sarcoidosis

Terapevticheskii arkhiv ◽

10.26442/terarkh201890342-46 ◽

2018 ◽

Vol 90 (3) ◽

pp. 42-46

Author(s):

V D Beketov ◽

M V Lebedeva ◽

N A Mukhin ◽

A G Serova ◽

A B Ponomarev ◽

...

Keyword(s):

Clinical Study ◽

Idiopathic Pulmonary Fibrosis ◽

Pulmonary Fibrosis ◽

Oxygen Saturation ◽

Clinical Significance ◽

Surfactant Proteins ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Individuals

The results of a clinical study showing the importance of surfactant proteins A and D in assessing the activity and progression of idiopathic pulmonary fibrosis and chronic lung sarcoidosis are presented. Aim. To study the clinical significance of SP-A, SP-D in assessing the activity of idiopathic pulmonary fibrosis and sarcoidosis. We examined 81 patients with morphologically confirmed diagnoses of idiopathic pulmonary fibrosis (ILF) and sarcoidosis, a control group of 20 healthy individuals. The MSCT of the thoracic organs of the chest was performed, the diffusivity of the lungs was examined, oxygen saturation was determined. In the serum, the surfactant proteins SP-A and SP-D were determined by the enzyme-linked immunosorbent assay. Results. A significant increase in SP-A and SP-D (p

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Activation and Functional Alteration of Mucosal-Associated Invariant T Cells in Adult Patients With Community-Acquired Pneumonia

Frontiers in Immunology ◽

10.3389/fimmu.2021.788406 ◽

2021 ◽

Vol 12 ◽

Author(s):

Lichen Ouyang ◽

Mi Wu ◽

Zhijun Shen ◽

Xue Cheng ◽

Wei Wang ◽

...

Keyword(s):

T Cells ◽

Peripheral Blood ◽

Lavage Fluid ◽

Community Acquired Pneumonia ◽

Healthy Individuals ◽

Cell Frequency ◽

Mait Cells ◽

Ifn Γ ◽

Invariant T Cells ◽

Mait Cell

Community-acquired pneumonia (CAP) remains the significant infectious cause of morbidity and mortality worldwide. Although mucosal-associated invariant T cells (MAIT) play roles in the pathogenesis of children CAP and ICU-associated pneumonia, their roles in adult CAP are largely unexplored. In this study, we investigated the frequency, phenotype, and function of MAIT cells in peripheral blood and bronchoalveolar lavage fluid (BALF) of adult CAP patients. Our data indicate that MAIT-cell frequency is profoundly lower in the peripheral blood of CAP patients compared to that in healthy individuals. Furthermore, the circulatory MAIT cells express higher levels of CD69 and PD-1 compared to those in healthy individuals. In BALF of CAP patients, MAIT-cell frequency is higher and MAIT cells express higher levels of CD69 and PD-1 compared to their matched blood counterparts. Levels of IL-17A and IFN-γ are increased in BALF of CAP patients compared to those in BALF of patients with pulmonary small nodules. The IL-17A/IFN-γ ratio is significantly positively correlated with MAIT frequency in BALF of CAP patients, suggesting a pathogenic role of MAIT-17 cells in CAP. Of note, blood MAIT-cell frequency in CAP patients is strongly negatively correlated with high-sensitivity C-reactive protein (hsCRP) and neutrophil count percentage in blood. The ability of circulating MAIT cells in CAP patients to produce IFN-γ is significantly impaired compared to those in healthy individuals. In summary, our findings suggest the possible involvement of MAIT cells in the immunopathogenesis of adult CAP.

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Presence of Bcr-Abl Positive Cells in Peripheral Blood Leukocytes of Immunosuppressed Solid Organ Transplant (SOT) Recipients

Blood ◽

10.1182/blood.v112.11.3191.3191 ◽

2008 ◽

Vol 112 (11) ◽

pp. 3191-3191

Author(s):

Philipp D. le Coutre ◽

Petra Reinke ◽

Ruth Neuhaus ◽

Ralf Trappe ◽

Frauke Ringel ◽

...

Keyword(s):

Peripheral Blood ◽

Case Reports ◽

Control Group ◽

Study Group ◽

Solid Organ ◽

Healthy Individuals ◽

Peripheral Blood Leukocytes ◽

Rt Pcr ◽

Blood Leukocytes ◽

Female Ratio

Abstract In chronic myeloid leukemia (CML) the p210 BCR-ABL protein, generated by a t(9;22)(q34;q11) translocation, is the underlying mechanism of leukemogenesis. Although the presence of p210 BCR-ABL is normally restricted to CML patients (pts), previous reports demonstrated low levels of bcr-abl transcripts in healthy individuals that may be controlled by an intact immune system (Bose et al. Blood92:3362, 1998). Interestingly, several articles reported cases of CML occurring in pts after SOT (Pelloso et al. Leukemia Res29:353, 2005). Therefore, immunosuppressed SOT recipients should represent an optimal population to investigate the frequency of bcr-abl transcripts in non-leukemic individuals in order to address a potential impact of immunosuppression on the presence of bcr-abl transcripts. This possibility was investigated by studying peripheral blood leukocytes for the presence of bcr-abl transcripts in a total of 201 individuals of whom 100 were SOT recipients (n=50 kidney, n=46 liver, n=3 heart, n=1 heart-lung) and 101 were control individuals (n=87 patients with renal failure, n=14 healthy individuals). The male to female ratio in the study group was 54:46 (median age: 55.38 years, range: 22–83) and matched the control group (median age: 59.8 years, range: 32–96). Included in the study group were 13 pts with post transplant malignancies who were previously treated with standard chemotherapeutic regimens. Immune suppressive drugs given to all SOT recipients included steroids (90%), cyclosporine A (69%), azathioprine (22%), tacrolimus (69%), sirolimus (44%), mycophenolic acid (63%) and others (37%). For the detection of the bcr-abl transcript we used a nested reverse transcriptase-polymerase chain reaction (RT-PCR) assay that is routinely used in our institution. All samples were tested at least twice. In 5/100 immunosuppressed pts at least 1 of 2 RT-PCR products was bcr-abl positive in the second round amplification. This rate significantly exceeded the control group that was completely bcr-abl negative (0/101 p = 0.0242). Of the 5 bcr-abl positive pts, 3 were liver transplant and 2 were kidney transplant recipients. The latency in this group (interval between transplantation and bcr-abl PCR) was at 58.4 months (range: 24-135 months) and shorter when compared to the latency in the remaining study group (104.8 months; range 1 – 369). Additionally, all samples were tested for both the pml-rara and aml1-eto transcripts that are detectable in subsets of pts with acute myeloid leukaemia but none of the samples tested positive for these transcripts. Our findings are extended by three case reports of SOT recipients (2 kidney, 1 liver) who developed CML in a total of 2088 transplantations in our centre in 9 years. In these three pts (2 males, 1 female), CML occurred at 84.3 months (range: 32–183) following SOT. In summary our data show: 1. The presence of bcr-abl transcripts in immunosuppressed non-leukemic SOT pts and the absence of bcr-abl transcripts in healthy normals when tested with routine PCR protocols. 2. The absence of pml-rara and aml1-eto transcripts in both the study and control group. And 3. the occurrence of CML in three patients following SOT suggesting a higher frequency of CML in this population.

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Peculiarities of cellular, humoral links of immunity and dynamics of immunoglobulins in community-acquired pneumonia in adults and their importance for diagnostics and prognosis of the disease

ScienceRise Medical Science ◽

10.15587/2519-4798.2021.237712 ◽

2021 ◽

pp. 13-16

Author(s):

Vladyslav Bereznyakov ◽

Oleksiy Korzh ◽

Sergiy Krasnokutskiy

Keyword(s):

Immune Status ◽

Community Acquired Pneumonia ◽

City Council ◽

Phagocytic Index ◽

Control Group ◽

Immune Disorders ◽

Healthy Individuals ◽

Non Profit ◽

Ig G ◽

Apparently Healthy

The aim of our work was to study the characteristics of the immune status based on the analysis of the cellular, humoral links of immunity and dynamics of immunoglobulins in adults with community-acquired pneumonia (CAP), and their importance in the pathogenesis and prognosis of the disease. Materials and methods. The study was carried out in the period 2017-2020 on the basis of the therapeutic department of the Municipal non-profit enterprise “City Clinical Multidisciplinary Hospital № 25” of Kharkiv City Council. The study involved 20 adult patients with CAP aged 18 to 80 years (mean age 36.5±10.3). The control group consisted of 20 apparently healthy individuals (mean age 39.5±12.5). The study of the immune status of patients was carried out by determining the phagocytic index, the content of lymphocytes, CD3+, CD4+, CD8+, CD16+, CD20+ and the level of immunoglobulins of classes A, G, M in the dynamics of the disease: on the first and tenth days after hospitalization. Results. On the first day of hospitalization, there was a significant imbalance in phagocytosis, T- and B-links of immunity. On the tenth day of treatment, the phagocytic index, the number of CD3+, CD4+, CD8+, CD16+ and CD20+ increased. An increase in Ig M, a decrease in Ig A and Ig G were also noted. Thus, standard therapy for patients with CAP leads to its clinical and radiological resolution, but is not accompanied by the normalization of immunity parameters. Conclusions. The features of immune disorders in patients with CAP were revealed: pneumonia increases the imbalance in the cellular link of immunity, the level of CD3+ and CD4+ decreases, there is no dynamics of the relative amount of CD20+. There are no significant changes in the humoral link of the immune status. For the treatment of patients with CAP, in addition to standard pharmacotherapy, it is necessary to include drugs that stimulate the immune system

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Effects of Different Oxygen Saturation on Activity of Complex Biomass and Aqueous Crude Extract of Cyanobacteria During Embryonal Development in Carp (Cyprinus carpio L.)

Acta Veterinaria Brno ◽

10.2754/avb200776020291 ◽

2007 ◽

Vol 76 (2) ◽

pp. 291-299 ◽

Cited By ~ 6

Author(s):

M. Palíková ◽

R. Krejčí ◽

K. Hilscherová ◽

B. Burýšková ◽

P. Babica ◽

...

Keyword(s):

Oxygen Saturation ◽

Aqueous Extract ◽

Cyprinus Carpio ◽

Crude Extract ◽

Oxygen Deficiency ◽

Dry Weight ◽

Control Group ◽

Embryonal Development ◽

Cyanobacterial Toxin ◽

Cyanobacterial Biomass

This study evaluates the effects of different oxygen concentrations on the toxicity of complex cyanobacterial biomass and aqueous extract of two cyanobacterial samples (prepared with regard to the content of the most studied cyanobacterial toxin microcystin: biomass 1 with the content of microcystins 2 560 μg l-1 of dry weight and biomass 2 with the content of microcystins 70 μg l-1 of dry weight, i.e. almost 37-times lower concentration) during embryonal development of carp (Cyprinus carpio). Effects of complex biomass and aqueous extract were tested at concentrations 120, 80 and 40 mg l-1 in four replicates, two of them aerated and two without aeration. The studied endpoints included the beginning and the end of embryo hatching, the presence of eye points at 48 h after fertilization, filling of air bladder, cumulative mortality and glutathione-S-transferase (GST) activities in surviving embryos. There was a significant increase in mortality in all variants without aeration after exposure to cyanobacterial biomass and aqueous extract (p < 0.01). The variants with aeration at greater cyanobacterial concentrations also lead to a significant mortality increase (p < 0.05). There was a decreased number of hatched embryos or no hatching at all in the non-aerated exposure variants for both biomasses. The lack of eye pigmentation 48 h after fertilization was observed at biomass concentrations of 120 and 80 mg l-1 for all non-aerated biomass exposures. There were fewer individuals with filled air bladder at the greatest tested concentrations of the complex biomass (biomass 1 and 2) and concentration of 80 mg l-1 (biomass 1) in the aerated variants. The activity of detoxification enzyme GST was studied during the experiment. GST activity was increased in treatments compared to control group in all aerated variants, but the changes were not always significant. The results of the conducted experiments clearly showed the important impact of oxygen saturation in water on the extent of cyanobacterial biomass toxicity. Mortality of fish eggs and embryos in experimental groups without aeration was significantly higher than in groups with aeration. Apart from the influence of toxic substances contained in the cyanobacterial biomass and the crude extract, oxygen deficiency affected the mortality in the groups without aeration.

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P5562Increased microRNA-21 gene expression levels as a biomarker of myocardial damage in acute myocarditis

European Heart Journal ◽

10.1093/eurheartj/ehz746.0506 ◽

2019 ◽

Vol 40 (Supplement_1) ◽

Author(s):

M Marketou ◽

J Kontaraki ◽

J Konstantinou ◽

S Maragkoudakis ◽

A Plevritaki ◽

...

Keyword(s):

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Acute Myocarditis ◽

Myocardial Damage ◽

Control Group ◽

Healthy Individuals ◽

Peripheral Blood Mononuclear ◽

Expression Levels ◽

Blood Mononuclear Cells

Abstract Purpose MicroRNAs (miRs) are implicated in various cardiovascular pathologies and are promising diagnostic and therapeutic targets. miR-21 plays a critical role in the regulation of inflammation and cardiac fibrosis. In this study, we evaluated miR-21 expression levels in peripheral blood mononuclear cells from patients with acute myocarditis compared to healthy individuals and explored their diagnostic potential as predictor of myocardial damage. Methods We assessed the expression levels of miR-21 in 55 patients with acute myocarditis (40 men, mean age 30±12 years) and 20 healthy individuals (15 men, mean age 30±9 years). Blood samples were taken on admission and miR-21 expression levels in peripheral blood mononuclear cells were quantified by real-time reverse transcription polymerase chain reaction. Plasma high sensitive troponine (TnI) was measured by immunoassay and a value above the laboratory reference range (11.6 pg/ml) was considered elevated. Results Myocarditis patients showed significantly higher troponine levels compared to healthy individuals (256.59±94.9 versus 11.9±9.01, p<0.001). miR-21 expression levels in peripheral blood mononuclear cells were significantly elevated in the myocarditis group compared to the control group (47.01±18.3 versus 3.8±2.2, p=0.02). In addition, miR-21 expression levels in peripheral blood mononuclear cells revealed a significant correlation with troponine levels in those patients (r=0.55, p<0.001) Conclusions Our data reveal that miR-21 is upregulated in peripheral blood mononuclear cells from patients with acute myocarditis relative to healthy individuals and is significantly correlated with myocardial damage in those patients. Our findings indicate that miR-21 may be involved in the pathophysiology of myocarditis and represent promising biomarker in the disease.

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