scholarly journals Potensi Aktivitas Bakteriosin Lactobacillus Gasseri Terhadap Pertumbuhan Salmonella Typhi

2021 ◽  
Vol 1 (3) ◽  
pp. 314-320
Author(s):  
Muhammad Rayhan Firdaus ◽  
Andani Eka Putra ◽  
Abdiana Abdiana

Latar belakang. Lactobacillus gasseri merupakan jenis bakteri asam laktat (BAL) yang dapat menghasilkan bakteriosin sebagai suatu senyawa antibakteri. Objektif. untuk mengetahui potensi aktivitas bakteriosin Lactobacillus gasseri terhadap pertumbuhan Salmonella typhi dengan menilai Kadar Hambat Minimal (KHM) dan Kadar Bunuh Minimal (KBM). Metode. Penelitian ini  dilakukan dengan teknik dilusi dan menggunakan desain Rancangan Acak Lengkap. Konsentrasi bakteriosin Lactobacillus gasseri dibagi dalam berbagai kelompok konsentrasi yaitu 100%, 80%, 60%, 40%, 20%, 10% dan 0% (kontrol) dengan empat kali pengulangan. Nilai KHM dan KBM ditentukan dengan menghitung jumlah koloni bakteri yang tumbuh dari masing-masing konsentrasi. Hasil penelitian dianalisis secara statistik dengan uji One-way ANOVA dan Post-Hoc LSD. Hasil. Hasil penelitian menunjukkan bahwa bakteriosin Lactobacillus gasseri dapat menghambat pertumbuhan Salmonella typhi  namun tidak ditemukan nilai KHM dan KBM. Dengan perhitungan statistik ditemukan perbedaan yang bermakna pada konsentrasi 100%, 80%, 60%, 40% dan 20% terhadap kontrol. Simpulan. bakteriosin Lactobacillus gasseri memiliki efek bakteriostatik terhadap pertumbuhan Salmonella typhi tetapi tidak memiliki efek bakterisidal. Kemampuan daya hambat bakteriosin Lactobacillus gasseri terhadap pertumbuhan Salmonella typhi dipengaruhi oleh konsentrasi bakteriosinya. Kata kunci:  Antibakteri, Bakteriosin, Lactobacillus gasseri, Salmonella typhi   Background. Lactobacillus gasseri is a type of lactic acid bacteria (BAL) that can produce bacteriocin as an antibacterial compound. Objective. To determine the potential activity of Lactobacillus gasseri bacteriocin on the growth of Salmonella typhi by assessing the Minimum Inhibitory Level (MIC) and the Minimum Bactericidal Concentration (MBC). Method. This research was conducted with the dilution technique and using a completely randomized design. The concentration of  Lactobacillus gasseri bacteriocin was divided into various concentration groups namely 100%, 80%, 60%, 40%, 20%, 10% and 0% (control) with four repetitions. The MIC and MBC values were determined by counting the number of bacterial colonies that grew from each concentration. The results of the study were statistically analyzed with the One-way ANOVA and Post-Hoc LSD tests. Results. The results showed that the bacteriocin Lactobacillus gasseri could inhibit the growth of Salmonella typhi but found no MIC and MBC values. Statistical calculations found significant differences in the concentration of 100%, 80%, 60%, 40% and 20% of the controls. Conclusion. bacteriocin Lactobacillus gasseri has a bacteriostatic effect on the growth of Salmonella typhi but has no bactericidal effect. The ability of Lactobacillus gasseri bacteriocin to inhibit the growth of Salmonella typhi is influenced by the concentration of bacteriocytes. Keyword: Antibacterial, Bacteriocin, Lactobacillus gasseri, Salmonella typhi

2018 ◽  
Author(s):  
Anita Dewi Moelyaningrum ◽  
Khoirul Ngibad ◽  
S.Pd Lilla Puji Lestari ◽  
Dessy Agustina Sari ◽  
Cahya Fajar Budi Hartanto ◽  
...  

This paper has been published in IOP Conf. Series: Journal of Physics: Conf. Ser1i2e3s415161748(9200‘1’8“)”012058 Lead (Pb) were toxic. Lead found in pipes, batteries, paint, dyes ceramic glaze, gasoline, and final waste disposal. The robusta coffee grouds residues had high carbon, nitrogen etc which can adsorb heavy metal.The purpose of this study is to analyze the robusta coffee grounds residues to adsorb the Pb in the water. The method of this research is a True Experimental using completely randomized design (CRD) method. There were control groups (C) and three treatments groups (T1; T2;T3; 5gram/ litre; 8gram/ litre; 10gram/ litre) with six repetitions. The robusta coffee ground residues were contacted for 2 hours.Total samples were24 samples which analyzed each parameter of the Pb with Atomic Adsorption Spectophotometry Analysis. The results showed that the more coffee ground residues that are exposed, increasingly turbid. The KS test showed that data were a normal distribution (sig=0,324). One way ANOVA test; Turkey post Hoc showed that there was sig difference between the control and treatment (F=4,326, Sig= 0,017). There were sig difference between control and treatment 2 and 3 (Sig=0,019; Sig=0,038). Robusta coffee grounds residues can reuse to adsorb the Pb pollution in the water. It can be a solution for treating the lead pollution in the water because of it easy to the application.


2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Henneke Pangkey ◽  
Sartje Lantu ◽  
Fanny Silooy

The purpose of this research was to investigate the population density of Alona sp. on yeast media with different concentrations. The study was conducted using a completely randomized design (CRD) consisting of 5 treatments and 3 replications for 21 days. The treatments given were A: 10 ppm yeast, B: 5 ppm yeast, C: 1 ppm yeast, D: horse manure, and E: well water. The initial stocking density of Alona sp. for each treatment was 15 individuals/300 mL of well water. The water quality parameter measured during the study was temperature. The results of the one-way ANOVA test showed that there were significant differences between treatments on the density level of Alona sp. (p <0.05). Tukey's further test showed that treatment B was the best with a density of Alona sp. as many as 8059 individuals/300 mL of well water. The temperature during the study, in the morning was 26 ± 0.52°C and in the afternoon was 26 ± 0.46°C.


2018 ◽  
Vol 24 (2) ◽  
pp. 135-140
Author(s):  
O.O.K Oko ◽  
P.O Ozung ◽  
F.B Abang

A 16-week trial evaluated the effects of ethanolic extract of Aspiliaafricana leaf (EeAaL) on the growth performance, carcass and egg characteristics of growing and laying quails. One hundred and eighty (180), one-week old Japanese quail chicks were assigned to six experimental diets (0, 2.5, 5, 7.5, 10% EeAaL or 0.02% oxytetracycline). Each treatment had 30 birdswhich were further subdivided into three replicates of 10 birds. Each replicate was housed in a pen under the deep litter system; feed and water were provided ad libitum.Growth performance and egg characteristics(egg quantity and quality) indices were subjected to the one-way ANOVA in a completely randomized design (CRD). No significant (P>0.05) effect of EeAaL was observed between treatments on the final body weight and daily weight gains of quails. Feed conversion ratio (4.95 – 5.28) was significantly (P<0.05) improved in quails fed 2.5 – 7.5% EeAaL diets. Zero mortality was observed in quails fed 5 – 10% EeAaL diets compared to other treatments. EeAaL supplementation reduced (P<0.05) the amount of fat deposition in quail carcass, thus improving the carcass quality. The efficacy ofEeAaL was more pronounced in laying than growing quails where significant (P<0.05) differences were observed on some external and internal egg characteristics, whereas egg composition did not differ (P>0.05) between treatments. Egg number, hen day production, albumen weight,yolk weight, shell thickness and yolk colour were significantly improved following dietary EeAaL  upplementation, indicative of strong growth promoting and stimulatory effects.Therefore, for improved carcass and egg performances up to 7.5% EeAaL shoud be supplemented intoquail diets.Keywords: Leaf extract, growth rate, egg characteristics, bush marigold, quails 


2020 ◽  
pp. 130-139
Author(s):  
Rela Sonia ◽  
Yusnelti Yusnelti ◽  
Fitrianingsih Fitrianingsih

Durian (Durio zibethinus Linn.) leaves are known to inhibit the xanthine oxidase enzyme which can reduce uric acid levels in the blood. The purpose of study is to determine the effectiveness of ethanol extracts of durian leaves in reducing uric acid levels in hyperuricemic mice. The mice were induced with chicken’s liver juice 0,2 mL/20 gBW and potassium oxonate 250 mg/kgBW. The animals were mice with uric acid levels above 3,3 mg/dL or 6,2-7,1 mg/dL. The study was used a completely randomized design (CRD) with six treatments and three replications, with three mice in each replications. The treatments group were K+: Allopurinol 10 mg/kgBW; K-: CMC-Na 0.5%; extracts in group P1,P2, P3, P4 are 50; 100; 200; 250 mg/kgBW. The measurements of uric acid levels were performed on day-0, day-6 after induction, and day-15 after administering each group. The data obtained were analyzed by One Way Anova test followed by Duncan's Post Hoc further test. The results showed that durian leaves could reduce uric acid levels in mice blood with the highest percentage reduction was in P2 (100 mg/kgBW) group of 40,53%, followed by P1 (50 mg/kgBW) of 38,68%. The lowest percentage was in the P4 group (250 mg/kgBW) of 22,36%, followed by the P3 group (200 mg/kgBW) of 27,71% and the ED50 value was 61,65 mg/kgBW. In conclusion, ethanol extracts of durian leaves has a significant effect on decreasing total uric acid levels in mice and has potential as an antihyperuricemia agent.


Author(s):  
Kusmardi Kusmardi ◽  
Berna Elya ◽  
Tri Wahyuni ◽  
Rafika Indah Paramita

Objective: Anemia in acute malaria occurs because there is massive lysis of erythrocytes as a result of an increase in parasitemia or use of drugs that may induce hemolysis. In this research, it was tested the effect of administration of a combination of sambiloto (Andrographis paniculata Nees) extract and spirulina (Arthrospira platensis Gomont). This combination is aimed to obtain antiparasite activity of sambiloto and increasing of hemoglobin (Hb) from spirulina.Methods: This research was conducted with a completely randomized design using 75 mice of Swiss Webster strain. Test groups were consisted of AP, AP+ES, AP+PS, carboxymethyl cellulose, and dihydroartemisinin-piperaquine. All mice were infected with Plasmodium berghei on the day 0. The test material extracts were given 3 days before infection (H-3) and every day for 28 days after infection. Parasitemia data were taken on day 3, 7, 10, 15, 21, and 28, while the data for erythrocyte count and Hb level were taken on day 3, 10, and 21. Data processing was conducted using one-way ANOVA, followed by post hoc test.Results: This study showed that combination of sambiloto extract and spirulina powder (AP+PS) gave a significant difference result in suppressing the percentage of parasitemia (p=0.02), increasing the erythrocyte count (p=0.03), and Hb level (p=0.01) at the 15th day, compared to the group given only sambiloto (AP).Conclusion: Combination of spirulina powder and sambiloto extract will decrease parasitemia and increase the number of erythrocyte and Hb in mice that infected by P. berghei.  


2020 ◽  
Vol 20 (1) ◽  
pp. 46
Author(s):  
Uswatun Hasanah ◽  
Ayu Adhita Damayanti ◽  
Fariq Azhar

Abstrak: Budidaya ikan badut Amphiprion ocellaris terus dikembangkan hingga saat ini guna menghasilkan ikan badut yang memiliki pertumbuhan, kelangsungan hidup dan kecerahan warna yang baik. Salah satu permasalahan dalam budidaya ikan badut adalah harga pakan yang cukup tinggi. Tujuan penelitian ini yaitu untuk mengetahui pengaruh laju pemuasaan secara periodik terhadap pertumbuhan, kelangsungan hidup dan kecerahan warna ikan badut Amphiprion ocellaris. Metode yang digunakan dalam penelitian ini adalah metode eksperimental dengan menggunakan Rancangan Acak Lengkap yang terdiri dari empat perlakuan dan tiga ulangan, yaitu P1 (tanpa pemuasaan), P2 (1 hari dipuasakan 1 hari diberi pakan), P3 (1 hari dipuasakan 2 hari diberi pakan), P4 (1 hari dipuasakan dan 3 hari diberi pakan) sehingga terdapat 12 unit total percobaan. Data yang diperoleh dianalisis menggunakan uji sidik ragam (One way Anova). Hasil penelitian yang diperoleh adalah pertumbuhan, kelangsungan hidup dan kecerahan warna ikan badut yang mengalami laju pemuasaan secara periodik tidak berbeda nyata (p>0.05) dengan ikan badut yang tidak dipuasakan. Hal ini menunjukkan bahwa ikan badut Amphiprion ocellaris dapat dipuasakan hingga periode 1 hari dipuasakan dan 1 hari diberi pakan yang mengalami total pemuasaan sebanyak 20 hari tanpa menurunkan pertumbuhannya. Pembudidaya ikan badut Amphiprion ocellaris direkomendasikan dapat menggunakan semua periode pemuasaan seperti yang diterapkan pada penelitian ini, namun untuk waktu pemuasaan yang lebih lama diperlukan penelitian lebih lanjut oleh para peneliti.   Kata kunci: Pemuasaan, ikan badut, pertumbuhan, kelangsungan hidup, kecerahan warnaAbstract: Amphiprion ocellaris clown fish cultivation continues to be developed today to produce clown fish that have good survival growth and colour brightness.  This study aims to determine the effect of periodic fasting rates on growth, survival and colour brightness of the Amphiprion ocellaris clown fish. The method used in this study is an experimental method using a Completely Randomized Design consisting of four treatments and three replications, namely P1 (without fasting), P2 (1 day fasted 1 day fed), P3 (1 day fasted 2 days fed), P4 (1 day fasted and 3 days fed) so that there are 12 total experimental units. The data obtained were analyzed using the one way Anova variance test. The results obtained were growth, survival and color brightness of clown fish that experienced periodic mastery rates were not significantly different (p> 0.05) from clown fish that were not fasted.This shows that the clown fish Amphiprion ocellaris can be fasted until a period of 1 day fasted and 1 day fed with a total mastery of 20 days without reducing growth. Amphiprion ocellaris clown fish farmers are recommended to be able to use all periods of mastery as applied in this study but for a longer mastery time further research is needed by esearchers.Keywords: Mastery, clown fish, growth, survival, colour brightness 


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Mahmudi Mahmudi ◽  
Hari Santoso ◽  
Saimul Laili

Natural insecticides made from lemongrass plants and zodia which are environmentally friendly insecticides and contribute to mosquito mortality. The purpose of this study was to determine the effect of lemongrass insecticides  (Cymbopogon nardus) and leaves of zodiac (Evodia suaveolens) on mosquito mortality (Aedes aegypti), and to determine the treatment of insecticides that were more effective against the mortality of mosquitoes. The research method used the experimental completely randomized design (CRD) with 4 treatments and 5 replications, namely treatment 1 control; treatment of 2 insecticides zodia; treatment of 3 lemongrass insecticides; and treatment of 4 combination lemongrass insecticides and zodia. The results showed that the control treatment did not effect, whereas in the treatment of zodia insecticides, lemongrass and combinations there were influences, presumably this happened because of difference in the chemical compounds of the 2 plants against mosquitoes. The results showed that mosquito mortality was controlled by 0%; mosquito mortality with zodia insecticide of 28%; mosquito mortality with lemongrass insecticide of 16.4%; and mosquito mortality with combination lemongrass insecticides and zodiac of 43.2%. The results of the One Way ANOVA test showed that there were significant differences and tended to experience increased mosquito mortality after being treated. Insecticides that are more effective in causing mosquito mortality are combination insecticides of lemongrass and zodia leaves.Keywords: Vegetable Insecticides, Lemongrass Plants, Zodia Plants, Mosquitoes.ABSTRAKInsektisida alami yang terbuat dari tanaman serai dan zodia merupakan insektisida alami yang ramah lingkungan dan berperan terhadap mortalitas nyamuk. Tujuan dari penelitian ini untuk mengetahui pengaruh insektisida serai (Cymbopogon nardus) dan daun zodia (Evodia suaveolens) terhadap mortalitas nyamuk (Aedes aegypti) dan untuk mengetahui perlakuan insektisida yang lebih efektif terhadap mortalitas nyamuk (Aedes aegypti). Metode penelitian menggunkan eksperimental Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu perlakuan 1 kontrol; perlakuan 2 insektisida zodia; perlakuan 3 insektisida serai; dan perlakuan 4 insektisida kombinasi serai dan zodia. Hasil penelitian menunjukkan perlakuan kontrol tidak ada pengaruh terhadap mortalitas nyamuk, sedangkan pada perlakuan insektisida zodia, serai serta kombinasi terdapat pengaruh, diduga hal tersebut terjadi karena adanya perbedaan kandungan senyawa kimia dari 2 tanaman tersebut terhadap nyamuk. Hasil penelitian menunjukkan mortalitas nyamuk perlakuan kontrol 0%; mortalitas nyamuk dengan insektisida zodia 28%; mortalitas nyamuk dengan insektisida serai 16,4%; dan mortalitas nyamuk dengan insektisida kombinasi serai dan zodia 43,2%. Hasil analisis uji One Way ANOVA menunjukkan terdapat perbedaan yang signifikan dan cenderung mengalami peningkatan mortalitas nyamuk setelah diberi perlakuan. Insektisida yang lebih efektif dalam mengakibatkan mortalitas pada nyamuk yaitu insektisida kombinasi serai dan daun zodia.Kata kunci: Insektisida Nabati, Tanaman Serai, Tanaman Zodia, Nyamuk


2020 ◽  
Vol 1 (2) ◽  
pp. 37-46
Author(s):  
Dian Fatriani Indah Saputri ◽  
Anwar Daud ◽  
Rachman Syah ◽  
Agus Bintara Birawida ◽  
Hasnawati Amqam ◽  
...  

Depuration is an effort to reduce/eliminate contamination including microplastics, which one is using a water circulation system. This study aims to determine the effective depuration time to reduce the microplastic content in Asaphis detlorata This study used a quantitative approach with experimental research design with a completely randomized design. There are 450 shells used as an experimental animal where is the treatment consisted of four depuration times, namely 1;2;3; and 4 days with 3 repetitions of each treatment, while the control shells were without depuration. Analysis of variance (ANOVA) was used to see the effect of depuration treatment on the microplastic content. If the effect of the treatment was significantly different, then the post hoc test was continued to determine the differences between treatments. The results showed that Asaphis detlorata obtained from the mouth of the Lakatong river estuary were contaminated with microplastics ranging from 0.6 to 8.1 MPs/shellfish and an average of 3.96 MPs/shellfish. Depuration time significantly affected the microplastic content in shellfish depuration effectiveness. There is a tendency that the longer depuration time is decreased microplastic content in shellfish. The effective depuration time to reduce the microplastic content in Asaphis detlorata was 3 and 4 days. Further research is needed for a more effective depuration for cleaning microplastics in shellfish.


2021 ◽  
Vol 40 (4) ◽  
pp. 340
Author(s):  
If'all If'all ◽  
Asriani Hasanuddin ◽  
Abdul Rahim ◽  
Syahraeni Kadir

Banggai yam plant (endemic in Banggai Kepulauan Regency) is a potential raw material in production of modified starch. The one of techniques to increase the value of starch is modification of starch by acetylation method. This study objectives to determine the physical, chemical and functional characteristics of Banggai acetate yam starch at various reaction times. The study used a Completely Randomized Design with treatment time reaction (T), namely T1: 30 min, T2: 35 min, T3: 40 min, T4: 45 min, T5: 50 min, T6: 55 min. This study was conducted to determine the characteristics of yam acetate starch, which includes percent acetyl, degree of substitution, water and oil holding capacity (WHC/OHC), flammability, solubility, moisture content, ash, fat, protein, starch and amylose starch. The results showed that the longer reaction time under certain conditions will increase the acetyl percent, degree of substitution, WHC, OHC, flareability, solubility but reduce levels of fat, water, ash, fat, protein, starch and amylose starch, yam acetate. In this study at the best reaction time is (50 min), gives the acetyl percent 8.658% and substitution degree of 0.356, the value of WHC, OHC, Swelling ratio and solubility of 32.21%, 30.21%, 1.98 g/g, and 19.17% respectively.


2020 ◽  
Vol 60 (1) ◽  
pp. 55-74
Author(s):  
Nikolas Nagy ◽  
Miroslav Holienka ◽  
Matej Babic ◽  
Jakub Michálek ◽  
Egon Kunzmann

SummaryThe main aim of this research was to compare differences in heart rate values (HR) of soccer players during small-sided games (SSGs) with different number of players. We assumed that the number of “neutral” player or players in small-sided games will significantly affect the intensity of SSGs and in this case the heart rate values of participating players. The experimental group consisted of older junior players from the FC DAC 1904 Dunajská Streda soccer club (n = 9). The heart rate values were evaluated on the basis of collected data, which we obtained using Polar sport testers and special software Polar Team2. In order to find out the statistical significance of the difference in heart rate was used the One-Way ANOVA and the Bonferroni post hoc test. The level of statistical significance we set at 5 %. We found out that with increasing number of “neutral” players’ the intensity of small-sided games gradually decreased. During SSG1 (3 vs. 3), we recorded the highest achieved average heart rate values of the monitored players, in average 171.33 ± 9.39 beats.min-1. This form of the SSGs was the most intense, but not statistically significant. Our recommendation is to employ SSGs in the systematic training process with different number of players, because we can adequately prepare the players for the match load itself. Attention need to be paid for the playing position requirements.


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