scholarly journals The Robusta coffee grounds residues to adsorb the heavy metal Lead (Pb) in the water

2018 ◽  
Author(s):  
Anita Dewi Moelyaningrum ◽  
Khoirul Ngibad ◽  
S.Pd Lilla Puji Lestari ◽  
Dessy Agustina Sari ◽  
Cahya Fajar Budi Hartanto ◽  
...  

This paper has been published in IOP Conf. Series: Journal of Physics: Conf. Ser1i2e3s415161748(9200‘1’8“)”012058 Lead (Pb) were toxic. Lead found in pipes, batteries, paint, dyes ceramic glaze, gasoline, and final waste disposal. The robusta coffee grouds residues had high carbon, nitrogen etc which can adsorb heavy metal.The purpose of this study is to analyze the robusta coffee grounds residues to adsorb the Pb in the water. The method of this research is a True Experimental using completely randomized design (CRD) method. There were control groups (C) and three treatments groups (T1; T2;T3; 5gram/ litre; 8gram/ litre; 10gram/ litre) with six repetitions. The robusta coffee ground residues were contacted for 2 hours.Total samples were24 samples which analyzed each parameter of the Pb with Atomic Adsorption Spectophotometry Analysis. The results showed that the more coffee ground residues that are exposed, increasingly turbid. The KS test showed that data were a normal distribution (sig=0,324). One way ANOVA test; Turkey post Hoc showed that there was sig difference between the control and treatment (F=4,326, Sig= 0,017). There were sig difference between control and treatment 2 and 3 (Sig=0,019; Sig=0,038). Robusta coffee grounds residues can reuse to adsorb the Pb pollution in the water. It can be a solution for treating the lead pollution in the water because of it easy to the application.

2019 ◽  
Vol 7 (1) ◽  
pp. 011
Author(s):  
Anita Dewi Moelyaningrum

Logam berat masih banyak ditemukan mencemari lingkungan. Cadmium adalah logam berat yang bisa ditemukan didalam air disekitar tempat pembuangan sampah. Penelitian ini bertujuan untuk menganalisis arang aktif ampas kopi dalam menurunkan kadar Cd dalam air. Metode penelitian ini adalah true eksperimental dengan desain Rancangan Acak Lengkap. Tahap pertama adalah pembuatan arang kemudian diaktivasi dengan HCl. Hasil Uji kualitas arang aktif ampas kopi meliputi rendemen (98,5%), kadar air (7%), kadar abu (4%) dan daya serap idoin (687,96 mg/g). Terdapat empat kelompok yaitu tanpa perlakuan 0 gr/l (P0), perlakuan dengan 5 gr/ l (P1), 8 gr/l (P2) dan 10g/l (P3),  dimana masing-masing kelompok terdiri dari 6 replikasi. Kelompok yang diberi perlakuan dikontakkan dengan arang aktif ampas kopi selama 2 jam. Selanjutnya dilakukan analisa kadar Cd dalam air dengan metode Spektrofotometri Serapan Atom. Hasil penelitian dilakukan uji normalitas kemudian dilakukan uji one way anova menggunakan SPSS. Hasil menunjukkan terdapat perbedaan secara signifikan sebesar 0,000 dengan interval kepercayaan 95% antara kelompok kontrol dan perlakuan. Arang aktif ampas kopi  secara significant dapat mengikat kadar cadmium dalam air. Heavy metal cadmium (Cd) is still polluted the environment. It can be found in the wells around the landfill. This study aimed to analyze the active charcoal coffee ground to adsorb the cadmium in the water. This research method is the true experimental design with Completely Randomized Design. The first stage is making charcoal and then activated with HCl. There are four groups 0 gram/littre (P0), perlakuan dengan 5 gram/littre (P1), 8 gram/littre (P2) dan 10 gram/littre (P3) with each group consisting of 6 replication. Treatement group contacted with active charcoal coffee around 2 hours. Results of research normality test later conducted ANOVA test. The test results informed that there is 0,000 difference significanly with 95% confidence intervals. The active charcoal coffee had significant to adsorb cadmium in the water.


2020 ◽  
pp. 130-139
Author(s):  
Rela Sonia ◽  
Yusnelti Yusnelti ◽  
Fitrianingsih Fitrianingsih

Durian (Durio zibethinus Linn.) leaves are known to inhibit the xanthine oxidase enzyme which can reduce uric acid levels in the blood. The purpose of study is to determine the effectiveness of ethanol extracts of durian leaves in reducing uric acid levels in hyperuricemic mice. The mice were induced with chicken’s liver juice 0,2 mL/20 gBW and potassium oxonate 250 mg/kgBW. The animals were mice with uric acid levels above 3,3 mg/dL or 6,2-7,1 mg/dL. The study was used a completely randomized design (CRD) with six treatments and three replications, with three mice in each replications. The treatments group were K+: Allopurinol 10 mg/kgBW; K-: CMC-Na 0.5%; extracts in group P1,P2, P3, P4 are 50; 100; 200; 250 mg/kgBW. The measurements of uric acid levels were performed on day-0, day-6 after induction, and day-15 after administering each group. The data obtained were analyzed by One Way Anova test followed by Duncan's Post Hoc further test. The results showed that durian leaves could reduce uric acid levels in mice blood with the highest percentage reduction was in P2 (100 mg/kgBW) group of 40,53%, followed by P1 (50 mg/kgBW) of 38,68%. The lowest percentage was in the P4 group (250 mg/kgBW) of 22,36%, followed by the P3 group (200 mg/kgBW) of 27,71% and the ED50 value was 61,65 mg/kgBW. In conclusion, ethanol extracts of durian leaves has a significant effect on decreasing total uric acid levels in mice and has potential as an antihyperuricemia agent.


2021 ◽  
Vol 7 (1) ◽  
pp. 74-84
Author(s):  
I Komang Alit Saputra ◽  
Ni Gusti Ayu Manik Ermayanti ◽  
A.A. S. A. Sukmaningsih

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.


2019 ◽  
Author(s):  
Anita Dewi Moelyaningrum

Jurnal Teknologi Lingkungan Lahan Basah, Vol. 02, No. 1, 2019: 011 - 019 Pemanfaatan Arang Aktif Ampas Kopi sebagai Adsorben Kadmium pada Air Sumur The Usage of Coffee Waste Actived Charcoal as Adsorbent of Cadmium in Well Water Puput Baryatik, Anita Dewi Moelyaningrum, Uswatun Asihta, Wita Nurcahyaningsih, Azzumrotul Baroroh, Herdian Riskianto. Bagian Kesehatan Lingkungan dan Kesehatan Keselamatan Kerja Fakultas Kesehatan Masyarakat Universitas Jember [email protected] Logam berat masih banyak ditemukan mencemari lingkungan. Cadmium (Cd) adalah logam berat yang bisa ditemukan di dalam air di sekitar tempat pembuangan sampah. Penelitian ini bertujuan untuk menganalisis arang aktif ampas kopi dalam menurunkan kadar Cd dalam air. Metode penelitian ini adalah true eksperimental dengan desain Rancangan Acak Lengkap. Tahap pertama adalah pembuatan arang kemudian diaktivasi dengan HCl. Hasil uji kualitas arang aktif ampas kopi meliputi rendemen (98,5%), kadar air (7%), kadar abu (4%) dan daya serap idoin (687,96 mg/g). Terdapat empat kelompok yaitu tanpa perlakuan 0 gr/l (P0), perlakuan dengan 5 gr/ l (P1), 8 gr/l (P2) dan 10g/l (P3), di mana masing-masing kelompok terdiri dari 6 replikasi. Kelompok yang diberi perlakuan dikontakkan dengan arang aktif ampas kopi selama 2 jam. Selanjutnya dilakukan analisa kadar Cd dalam air dengan metode Spektrofotometri Serapan Atom. Hasil penelitian dilakukan uji normalitas kemudian dilakukan uji one way anova menggunakan SPSS. Hasil menunjukkan terdapat perbedaan secara signifikan sebesar 0,000 dengan interval kepercayaan 95% antara kelompok kontrol dan perlakuan. Arang aktif ampas kopi secara signifikan dapat mengikat kadar kadmium dalam air. Heavy metal cadmium (Cd) is still polluted the environment. It can be found in the wells around the landfill. This study aimed to analyze the active charcoal coffee ground to adsorb the cadmium in the water. This research method is the true experimental design with Completely Randomized Design. The first stage is making charcoal and then activated with HCl. There are four groups 0 gram/littre (P0), perlakuan dengan 5 gram/littre (P1), 8 gram/littre (P2) dan 10 gram/littre (P3) with each group consisting of 6 replication. Treatement group contacted with active charcoal coffee around 2 hours. Results of research normality test later conducted ANOVA test. The test results informed that there is 0,000 difference significanly with 95% confidence intervals. The active charcoal coffee had significant to adsorb cadmium in the water.


Author(s):  
Kusmardi Kusmardi ◽  
Berna Elya ◽  
Tri Wahyuni ◽  
Rafika Indah Paramita

Objective: Anemia in acute malaria occurs because there is massive lysis of erythrocytes as a result of an increase in parasitemia or use of drugs that may induce hemolysis. In this research, it was tested the effect of administration of a combination of sambiloto (Andrographis paniculata Nees) extract and spirulina (Arthrospira platensis Gomont). This combination is aimed to obtain antiparasite activity of sambiloto and increasing of hemoglobin (Hb) from spirulina.Methods: This research was conducted with a completely randomized design using 75 mice of Swiss Webster strain. Test groups were consisted of AP, AP+ES, AP+PS, carboxymethyl cellulose, and dihydroartemisinin-piperaquine. All mice were infected with Plasmodium berghei on the day 0. The test material extracts were given 3 days before infection (H-3) and every day for 28 days after infection. Parasitemia data were taken on day 3, 7, 10, 15, 21, and 28, while the data for erythrocyte count and Hb level were taken on day 3, 10, and 21. Data processing was conducted using one-way ANOVA, followed by post hoc test.Results: This study showed that combination of sambiloto extract and spirulina powder (AP+PS) gave a significant difference result in suppressing the percentage of parasitemia (p=0.02), increasing the erythrocyte count (p=0.03), and Hb level (p=0.01) at the 15th day, compared to the group given only sambiloto (AP).Conclusion: Combination of spirulina powder and sambiloto extract will decrease parasitemia and increase the number of erythrocyte and Hb in mice that infected by P. berghei.  


2021 ◽  
Vol 1 (3) ◽  
pp. 314-320
Author(s):  
Muhammad Rayhan Firdaus ◽  
Andani Eka Putra ◽  
Abdiana Abdiana

Latar belakang. Lactobacillus gasseri merupakan jenis bakteri asam laktat (BAL) yang dapat menghasilkan bakteriosin sebagai suatu senyawa antibakteri. Objektif. untuk mengetahui potensi aktivitas bakteriosin Lactobacillus gasseri terhadap pertumbuhan Salmonella typhi dengan menilai Kadar Hambat Minimal (KHM) dan Kadar Bunuh Minimal (KBM). Metode. Penelitian ini  dilakukan dengan teknik dilusi dan menggunakan desain Rancangan Acak Lengkap. Konsentrasi bakteriosin Lactobacillus gasseri dibagi dalam berbagai kelompok konsentrasi yaitu 100%, 80%, 60%, 40%, 20%, 10% dan 0% (kontrol) dengan empat kali pengulangan. Nilai KHM dan KBM ditentukan dengan menghitung jumlah koloni bakteri yang tumbuh dari masing-masing konsentrasi. Hasil penelitian dianalisis secara statistik dengan uji One-way ANOVA dan Post-Hoc LSD. Hasil. Hasil penelitian menunjukkan bahwa bakteriosin Lactobacillus gasseri dapat menghambat pertumbuhan Salmonella typhi  namun tidak ditemukan nilai KHM dan KBM. Dengan perhitungan statistik ditemukan perbedaan yang bermakna pada konsentrasi 100%, 80%, 60%, 40% dan 20% terhadap kontrol. Simpulan. bakteriosin Lactobacillus gasseri memiliki efek bakteriostatik terhadap pertumbuhan Salmonella typhi tetapi tidak memiliki efek bakterisidal. Kemampuan daya hambat bakteriosin Lactobacillus gasseri terhadap pertumbuhan Salmonella typhi dipengaruhi oleh konsentrasi bakteriosinya. Kata kunci:  Antibakteri, Bakteriosin, Lactobacillus gasseri, Salmonella typhi   Background. Lactobacillus gasseri is a type of lactic acid bacteria (BAL) that can produce bacteriocin as an antibacterial compound. Objective. To determine the potential activity of Lactobacillus gasseri bacteriocin on the growth of Salmonella typhi by assessing the Minimum Inhibitory Level (MIC) and the Minimum Bactericidal Concentration (MBC). Method. This research was conducted with the dilution technique and using a completely randomized design. The concentration of  Lactobacillus gasseri bacteriocin was divided into various concentration groups namely 100%, 80%, 60%, 40%, 20%, 10% and 0% (control) with four repetitions. The MIC and MBC values were determined by counting the number of bacterial colonies that grew from each concentration. The results of the study were statistically analyzed with the One-way ANOVA and Post-Hoc LSD tests. Results. The results showed that the bacteriocin Lactobacillus gasseri could inhibit the growth of Salmonella typhi but found no MIC and MBC values. Statistical calculations found significant differences in the concentration of 100%, 80%, 60%, 40% and 20% of the controls. Conclusion. bacteriocin Lactobacillus gasseri has a bacteriostatic effect on the growth of Salmonella typhi but has no bactericidal effect. The ability of Lactobacillus gasseri bacteriocin to inhibit the growth of Salmonella typhi is influenced by the concentration of bacteriocytes. Keyword: Antibacterial, Bacteriocin, Lactobacillus gasseri, Salmonella typhi


2020 ◽  
Vol 1 (2) ◽  
pp. 37-46
Author(s):  
Dian Fatriani Indah Saputri ◽  
Anwar Daud ◽  
Rachman Syah ◽  
Agus Bintara Birawida ◽  
Hasnawati Amqam ◽  
...  

Depuration is an effort to reduce/eliminate contamination including microplastics, which one is using a water circulation system. This study aims to determine the effective depuration time to reduce the microplastic content in Asaphis detlorata This study used a quantitative approach with experimental research design with a completely randomized design. There are 450 shells used as an experimental animal where is the treatment consisted of four depuration times, namely 1;2;3; and 4 days with 3 repetitions of each treatment, while the control shells were without depuration. Analysis of variance (ANOVA) was used to see the effect of depuration treatment on the microplastic content. If the effect of the treatment was significantly different, then the post hoc test was continued to determine the differences between treatments. The results showed that Asaphis detlorata obtained from the mouth of the Lakatong river estuary were contaminated with microplastics ranging from 0.6 to 8.1 MPs/shellfish and an average of 3.96 MPs/shellfish. Depuration time significantly affected the microplastic content in shellfish depuration effectiveness. There is a tendency that the longer depuration time is decreased microplastic content in shellfish. The effective depuration time to reduce the microplastic content in Asaphis detlorata was 3 and 4 days. Further research is needed for a more effective depuration for cleaning microplastics in shellfish.


2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Henneke Pangkey ◽  
Sartje Lantu ◽  
Fanny Silooy

The purpose of this research was to investigate the population density of Alona sp. on yeast media with different concentrations. The study was conducted using a completely randomized design (CRD) consisting of 5 treatments and 3 replications for 21 days. The treatments given were A: 10 ppm yeast, B: 5 ppm yeast, C: 1 ppm yeast, D: horse manure, and E: well water. The initial stocking density of Alona sp. for each treatment was 15 individuals/300 mL of well water. The water quality parameter measured during the study was temperature. The results of the one-way ANOVA test showed that there were significant differences between treatments on the density level of Alona sp. (p <0.05). Tukey's further test showed that treatment B was the best with a density of Alona sp. as many as 8059 individuals/300 mL of well water. The temperature during the study, in the morning was 26 ± 0.52°C and in the afternoon was 26 ± 0.46°C.


2021 ◽  
Vol 6 (1) ◽  
pp. 43
Author(s):  
Yulistiati Nengsih ◽  
Aditya Deska Wahyu

Indonesia is one of the largest coffee producing and exporting countries in the world. Most of the production is robusta variety. Coffee is the fourth largest foreign exchange earner for Indonesia after palm oil, rubber and cocoa and is a source of income for the community. Coffee can be developed in a generative (seed) and vegetative way, including using cuttings. Efforts to accelerate the formation of roots in cuttings can be done by using natural growth regulators, one of which is shallots which contain auxins. The purpose of this study was to examine the effect of shallot extract on the growth of robusta coffee cuttings (Coffea canephora). This research was conducted from December 2019 to February 2020 at Campus II, Batanghari University (Pijoan). The design used in this study was a completely randomized design (CRD) with one treatment factor, namely shallot extract including; .k0 (without giving onion extract), k1 (250 g of shallots in 1 liter of distilled water), k2 (500 g of shallots in 1 liter of distilled water), k3 (750 g of shallots in 1 liter of distilled water), and k4 (1000 g of shallots in 1 liter of distilled water). Each stage of the experiment was repeated 3 times, so that there were 15 experimental plots, for each experiment there were 10 cuttings with a total of 150 cuttings. The observed data were analyzed using analysis of variance followed by the DNMRT test at α 5%. From the immersion treatment in 750 g of shallot extract in 1 liter of distilled water (k3), there was an increase in the percentage of live cuttings 47.37%, the percentage of shoots and roots growth of 83.51%, 106.06% of shoot length, and 98.03% of roots. , and the root dry weight of 225.27% compared without giving onion extract (k0).


2014 ◽  
Vol 1 (1) ◽  
pp. 57
Author(s):  
Juniaty Towaha ◽  
Asif Aunillah ◽  
Eko Heri Purwanto ◽  
Handi Supriadi

<p>Kopi Robusta Indonesia memiliki agroklimat dan elevasi tempat yang variatif serta lebih luas sehingga berpotensi sebagai penghasil kopi Robusta yang bermutu tinggi dengan citarasa dan aroma khas. Penelitian telah dilaksanakan di perkebunan rakyat Provinsi Lampung dari bulan Januari hingga Desember 2013. Tujuan penelitian adalah menganalisis pengaruh elevasi dan pengolahan terhadap kandungan kimia serta citarasa kopi Robusta di perkebunan kopi Robusta milik rakyat di Provinsi Lampung. Penelitian menggunakan metode survey dan analisis datanya mengikuti Rancangan Acak Lengkap dengan dua faktor dan tiga ulangan. Faktor pertama adalah empat ketinggian tempat, yaitu (1) 200; (2) 400; (3) 600 dan (4) 800 m dpl, sedangkan faktor kedua pengolahan buah kopi, yaitu (1) basah dan (2) kering. Parameter yang diamati meliputi pengujian kadar kafein, protein, lemak, dan abu serta uji organoleptik (cupping test). Hasil penelitian mendapatkan bahwa makin tinggi elevasi tempat tumbuh kopi Robusta di daerah Lampung maka kadar kafein dan lemak cenderung semakin meningkat. Selanjutnya, proses pengolahan kopi secara basah menghasilkan mutu citarasa kopi Robusta Lampung lebih tinggi dibandingkan dengan pengolahan secara kering.</p><p>Kata Kunci: Kopi Robusta, elevasi, pengolahan, kandungan kimia, citarasa</p><p>Robusta coffee was grown in Indonesia at diverse agro-climatic conditions and altitudes, so it potentially to develop of high quality Robusta coffee with a distinctive flavor and aroma. Research was conducted on smallholder plantations in Lampung Province from January to December 2013. The objective of this study was to analyze the quality and flavor of Robusta coffee developed at different elevation in Lampung Province, in order to identify the most appropriate elevation for Robusta coffee to have the best quality and flavor. Research was use completely randomized design with three replications and two factors. The first factor is altitude: (1) 200; (2) 400; (3) and 600 (4) 800 m above sea level, while the second factor is the processing technique: (1) wet; and (2) dry processing. Variables tested were levels of caffeine, protein, fat and ash as well as cup quality. The results showed that the higher of elevation the higher of caffein and fat contents. Moreover, wet processing of Lampung Robusta Coffee gave higher cup quality compared to dry processing.</p>


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