scholarly journals SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BATANG (Eusideroxylon zwageri) TERHADAP Escherichia coli DAN Salmonella typhi

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hana Wila ◽  
Fathul Yusro ◽  
Yeni Mariani
Keyword(s):  
Escherichia Coli ◽  
Moisture Content ◽  
Typhoid Fever ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Phytochemical Analysis ◽  
Strong Inhibition ◽  
E Coli ◽  
Growth Of Bacteria ◽  
Eusideroxylon Zwageri

Diarrhea and typhoid fever are diseases commonly suffered by people in Indonesia, and bacteria such as Escherichia coli (diarrhea) and Salmonella typhi (typhoid fever) are one of those diseases causative agents. Various plants are potentially used as anti-bacterial including ulin (Eusideroxylon zwageri). The aims of this study were to analysis the yield and potential secondary metabolite content in ethanol extract of E. zwageri, and determine the optimal concentration in inhibits the growth of bacteria E. coli and S. typhi. The methods used in this study were measure the moisture content of E. zwageri wood bark powder, extraction with ethanol solvent, measure the yield percentage, qualitative of phytochemical analysis, and antibacterial assays. The results showed that the yield of ethanol extract of E. zwageri is 21,63% with 7,6% of moisture content. The extract positively contained alkaloids, flavonoids, tannins, saponins, phenolics and terpenoids. The optimum concentration in inhibits the growth of bacteria E. coli is 200 mg/ml and categorized as very strong inhibition response, and 15 mg/ml is concentration used in inhibits the S. typhi and categorized as strong inhibition response.Keywords: Antibacterial activity, Escherichia coli, Eusideroxylon zwageri, phytochemical screening, Salmonella typhi.

scholarly journals UJI EFEKTIFITAS ANTIBAKTERI EKSTRAK KULIT BATANG JAMBU MONYET (Bellucia pentamera Naudin) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Salmonella typhi

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Farid Priandi ◽  
Fathul Yusro ◽  
Farah Diba ◽  
Yeni Mariani ◽  
. Nurhaida
Keyword(s):  
Escherichia Coli ◽  
Typhoid Fever ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Negative Effects ◽  
E Coli ◽  
Extract Concentration ◽  
The People ◽  
The Media

People still use plants as a medicine to overcome a disease, because it is easily to obtain and relatively does not provide significant negative effects. One of the plants utilized by the people is Bellucia pentamera Naudin (B. pentamera). Diarrhea and typhoid fever are diseases that often assault Indonesian people. The bacterias were used in this study were Escherichia coli (E. coli) and Salmonella typhi (S. typhi). This study aimed to analyze the antibacterial effectiveness of B. pentamera bark extracts against E. coli and S. typhi, by Kirby-Bauer diffusion method with PCA as the media. The highest extract concentration against E. coli is 400 mg/ml possess an inhibition of 20±1 mm, and towards S. typhi is 80 mg/ml which possess an inhibition of 24.67±0,58 mm.Keywords: Bellucia pentamera, Disc Diffussion, Efectiveness, Escherichia coli, Salmonella typhi.

scholarly journals AKTIVITAS EKSTRAK METANOL DAUN ULIN (EUSIDEROXYLON ZWAGERI TEIJSM & BINN) TERHADAP EMPAT JENIS BAKTERI PATOGEN

2020 ◽  
Vol 20 (1) ◽  
pp. 94
Author(s):  
Yeni Mariani ◽  
Fathul Yusro ◽  
Evy Wardenaar
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
West Kalimantan ◽  
E Coli ◽  
Eusideroxylon Zwageri

Abstrak: Suku Dayak Uud Danum di Kalimantan Barat memanfaatkan daun Ulin (Eusideroxylon zwageri Teijsm & Binn) sebagai tumbuhan obat dalam pengobatan tradisional untuk mengobati berbagai penyakit seperti diare, demam dan penguat badan. Penelitian ini dilakukan untuk menganalisis aktivitas ekstrak metanol dari daun ulin terhadap beberapa jenis bakteri patogen. Daun ulin diekstraksi dengan menggunakan metanol, kemudian diuji dengan metode difusi terhadap empat jenis bakteri yaitu Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi pada empat level konsentrasi (1, 5, 10 dan 15 mg/ml) dan E. coli (50, 100, 150 dan 200 mg/ml). Hasil pengujian memperlihatkan bahwa semua level konsentrasi berpengaruh signifikan terhadap hambatan pertumbuhan bakteri patogen. Semakin tinggi konsentrasi ekstrak metanol daun ulin semakin tinggi diameter hambat yang dihasilkan. Diameter hambat tertinggi ditunjukkan oleh konsentrasi 15 mg/ml pada jenis bakteri S. typhi (12,33 mm) dan E. coli pada konsentrasi 200 mg/ml (22,67 mm). Hasil pengujian membuktikan bahwa daun ulin yang secara tradisional digunakan dalam pengobatan tradisional memiliki potensi sebagai antibakteri alami.Kata kunci: Ulin, antibakteri, Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi, dan Escherichia coli.Abstract: Dayak Uud Danum of West Kalimantan traditionally use ulin leaves (Eusideroxylon zwageri Teisjm & Binn) to cure various diseases namely diarrhea, fever, and tonic. This present study aims to evaluate the activity of methanol extract of ulin leaves against several types of pathogenic bacteria. Ulin leaves were extracted with methanol; thus, the extract resulted tested in terms of disc diffusion Kirby and Bauer against four types of bacteria such as Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi (1, 5, 10, and 15 mg/ml), and Escherichia coli (50, 100, 150, and 200 mg/ml). The results showed that all levels of concentration significantly affect the inhibition growth of pathogenic bacteria. The higher the concentration of methanol extract of ulin leaves used, the higher the inhibition zone produced. The highest response inhibition growth showed from the highest level of 15 mg/ml on S. tyhpi (12.33 mm), and E. coli was 200 mg/ml (22.67 mm). It suggested that the leaves of ulin which traditionally used as medicinal plant have the potency as natural antibacterial agents.Keywords: Ulin, antibacterial, Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi, and Escherichia coli.

scholarly journals Phytochemical Analysis and Antibacterial Activity of Moringa oleifera Leaves Extracts against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

2021 ◽  
pp. 34-43
Author(s):  
Sheriffdeen Bale Issa ◽  
Muhyiddeen Muazu ◽  
Isma’il Rabi’u
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
E Coli ◽  
Ethanol Extracts

This study tests the antibacterial activities of Moringa oleifera leaf extract against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, obtained from Microbiology laboratory, Al-Hikmah University Ilorin. Phytochemical analysis reveals the presence of tannins in all the three extracts (Aqueous, ethanolic and N-hexane), while. flavonoids, alkaloids and Saponins were only present in the Aqueous and ethanolic extracts. The leaves extracts were screened for antibacterial activity by agar well diffusion method, employing five different extracts concentrations (100mg/ml, 80mg/ml, 60mg/ml, 40mg/ml and 20mg/ml). The aqueous extracts had a mean activity of 11.50±0.70mm, 7.50±0.70mm and 8.5±0.70mm for S. aureus, E. coli and P. aeruginosa respectively. The ethanol extracts had a mean activity of 12.00±1.41mm, 10.00±1.41mm and 8.00±1.21mm for S. aureus, E. coli and P. aeruginosa respectively, while the N-hexane extracts exert no any activity. The antibiotics discs exert more inhibitory effect compared to the extract. The MIC for the aqueous extracts are at a concentrations of 60mg/ml for S. aureus, 80mg/ml for P. aeruginosa and 80mg/ml for E. coli. While that of the ethanol extract of the leaves are at a concentration of 60mg/ml for S. aureus, 80mg/ml for E. coli and 100mg/ml for P. aeruginosa. The MBC for the aqueous Extract of the leaves are at a concentration of 80mg/ml for S. aureus, 100mg/ml for P. aeruginosa and 100mg/ml for E. coli, while that of the ethanol extract are at a concentration of 80mg/ml for S. aureus and 100mg/ml for E. coli), while no any MBC was recorded for P. aeruginosa. The antibacterial activity tests indicate that the ethanol extract was more active. Among the three isolates, S. aureus is more sensitive to the aqueous and ethanol extracts. The activity exhibited by the extracts may be related to the presence of a number of Phytoconstituents.

scholarly journals Níveis de sensibilidade de enterobactérias, em particular Salmonella typhi, a rifamicina S.V.

1969 ◽  
Vol 3 (2) ◽  
pp. 87-93
Author(s):  
Ivone R. Suassuna ◽  
I. Suassuna ◽  
C. E. de V. Serpa
Keyword(s):  
Escherichia Coli ◽  
Proteus Mirabilis ◽  
Salmonella Typhi ◽  
E Coli

Em face à predominante eliminação biliar da rifamicina S.V. atingindo concentrações muitas vêzes superiores aos níveis séricos obtidos com as doses terapêuticas, e pelo possível interêsse dessa verificação para o tratamento dos portadores biliares crônicos de Salmonella typhi determinou-se a concentração mínima inibitória de 165 estirpes de enterobactérias, incluindo 77 amostras de S. typhi. Foi verificado que a maioria das cepas de Escherichia coli, Shigella e Proteus mirabilis correspondiam a uma concentração inibitória mínima entre 33 a 65 μg/ml. Entre 65 e 128 μg/ml foram determinadas as concetrações inibitórias mínimas da maioria das outras espécies de Proteus, de Providencia e de Klebsiella. Para Salmonella e Enterobacter o limite mínino de sensibilidade foi, em regra, igual ou superior a 128 μg/ml. Diferenças mais acentuadas de comportamento entre as enterobactérias foram observadas quanto à ação bactericida da rifamicidas S.V. De uma maneira geral, para E. coli e Shigella, as concentrações inibitórias mínimas já referidas. Para as espécies de Proteus e Providencia houve variação maior de comportamento, mas tendência a que o efeito bactericidas fôsse encontrado em concentrações que correspondiam a 4 vêzes as bacteriostáticas para as mesmas espécies. Finalmente, de modo pouco feliz para os propósitos visados, em Salmonella, com a inclusão de S. typhi, não foi atingido um efeito bactericida, com as mais altas concentrações usadas as quais corresponderam em média a 6 vêzes as concentrações bacteriostáticas para esse gênero.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine polmifolia (L.) Merr) TERHADAP Salmonella typhi dan Escherichia coli

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nofita Nofita ◽  
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Skin Infections ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Agar Diffusion ◽  
Escherichia Coli Bacteria

Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC

scholarly journals Phytochemical analysis and antibacterial activity of Khaya senegalensis bark extracts on Bacillus subtilis, Escherichia coli and Proteus mirabilis

2016 ◽  
Vol 8 (3) ◽  
pp. 333 ◽  
Author(s):  
Abdullahi Aliyu ◽  
Alkali BR ◽  
Yahaya MS ◽  
Garba A ◽  
Adeleye SA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Proteus Mirabilis ◽  
Traditional Healers ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
E Coli ◽  
Khaya Senegalensis ◽  
Ethanol Extracts

<p>The aqueous and ethanol extracts of the bark of<em> Khaya senegalensis</em> were screened for their phytochemical constituents and preliminary antibacterial activity against <em>Bacillus subtilis, Escherichia coli</em> and<em> Proteus mirabilis. </em>The minimum inhibitory concentration (MIC) of the plant on the tested organisms was determined using multiple tubes method.</p><p>Alkaloids, anthraquinones, glycosides, tannins and steroids were detected in both extracts.</p><p>The ethanol and aqueous extracts of the plant showed antibacterial activity against <em>B. subtilis and E. coli,</em> with the aqueous extracts having more activity than those of ethanol. However the growth of<em> P. mirabilis</em> was not inhibited by either of the extracts. The MIC value was determined to be 50 mg/ml for<em> B. subtilis </em>and<em> E. coli. </em>The results are suggestive of considerable antibacterial activity of<em> K. senegalensis </em>and may justify its use in the treatment of bacterial diseases by herbalists or traditional healers.</p>

scholarly journals Transfer of Escherichia Coli to Lemons Slices and Ice during Handling

2017 ◽  
Vol 6 (4) ◽  
pp. 111
Author(s):  
Paul Dawson ◽  
Inyee Han ◽  
Ahmet Buyukyavuz ◽  
Wesam Aljeddawi ◽  
Rose Martinez-Dawson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Room Temperature ◽  
Separate Experiment ◽  
E Coli ◽  
And Storage ◽  
Growth Of Bacteria

The objective of this study was to determine the transfer and survival of bacteria during the handling and storage of lemons and transfer of bacteria during handling of ice. Ice and lemon slices are handled and stored in public eating places and used in beverages. During handling and storage the contamination and growth of bacteria may occur leading to the spread of disease. To fulfill the objective, hands were inoculated with Escherichia coli prior to handling of wet and dry whole lemons and in a separate experiment, ice cubes were handled. E. coli transferred to whole lemons or ice after handling were determined. The CFU per lemon and percentage of E. coli transferred were greater for wet lemons -6123 cfu and 4.62% compared to 469 cfu and .2% for dry lemons. The second experiment found from 2 to 67% of the bacteria on hands were transferred to ice by hands and from 30 to 83% of the bacteria on scoops were transferred to ice. In a third experiment, lemons were inoculated with E. coli, then sliced and stored at 4 or 22C and tested at 0, 4 and 24 hr. Lemons stored at room temperature (22°C) had an increase in E. coli population after 24 hour while those stored under refrigeration had a decrease even though bacteria did survive on lemons in either case. 

scholarly journals ANTI DIARRHEA EFFECT OF ETHANOL EXTRACT KECAPI BARK (SANDORICUM KOETJAPE MERR) ON MALE GUINEA PIG INDUCED WITH CASTOR OIL AND BACTERIA ESCHERICHIA COLI

2018 ◽  
Vol 11 (13) ◽  
pp. 73
Author(s):  
Eva Diansari ◽  
Edy Suwarso ◽  
Aminah Dalimunthe
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Guinea Pig ◽  
Castor Oil ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Start Time ◽  
E Coli ◽  
Sandoricum Koetjape

Objective: Kecapi bark (Sandoricum koetjape Merr.) is a plant of the Meliaceae family that is often used by people to treat diarrhea and abdominal pain. This study aims to determine the effects of antidiarrheal from the ethanol extract of the Kecapi stem bark on induced with castor oil and bacteria Escherichia coli.Methods: Before the in vivo on male guinea pig test was switched first, then 10 ml of castor oil was induced and 7.7 ml of E. coli male guinea pig was induced, then each male guinea was given a variation of dose ethanol extract kecapi stem bark and loperamide as the comparison. Parameters measured in this study were the start time of diarrhea, consistency of feces, frequency, and duration of diarrhea.Result: The results obtained showed that ethanol extract of kecapi bark antidiarrheal effect on guinea pigs induced castor oil (dose of 800 mg/kg body weight) and E. coli.Conclusion: Ethanol extract of Kecapi bark has an activity of antidiarrheal..

Fate of Escherichia coli O157:H7 during Composting of Bovine Manure in a Laboratory-Scale Bioreactor

2003 ◽  
Vol 66 (1) ◽  
pp. 25-30 ◽  
Author(s):  
XIUPING JIANG ◽  
JENNIE MORGAN ◽  
MICHAEL P. DOYLE
Keyword(s):  
Escherichia Coli ◽  
Moisture Content ◽  
Enrichment Culture ◽  
Escherichia Coli O157 ◽  
External Temperature ◽  
E Coli ◽  
Self Heating ◽  
Large Populations ◽  
Constant Moisture Content ◽  
Carbon Nitrogen Ratio

Inactivation profiles of Escherichia coli O157:H7 in inoculated bovine manure–based compost ingredients were determined by composting these ingredients in a bioreactor under controlled conditions. A 15-liter bioreactor was constructed to determine the fate of E. coli O157:H7 and changes in pH, moisture content, temperature, and aerobic mesophilic and thermophilic bacterial counts during composting. Fresh cow manure, wheat straw, cottonseed meal, and ammonium sulfate were combined to obtain a moisture content of ca. 60% and a carbon/nitrogen ratio of 29:1. The compost ingredients were held in the bioreactor at a constant external temperature of 21 or 50°C. Self-heating of the ingredients due to microbial activity occurred during composting, with stratified temperatures occurring within the bioreactor. At an external temperature of 21°C, self-heating occurred for 0 to 3 days, depending on the location within the bioreactor. E. coli O157:H7 populations increased by 1 to 2 log10 CFU/g during the initial 24 h of composting and decreased by ca. 3.5 log10 CFU/g near the bottom of the bioreactor and by ca. 2 log10 CFU/g near the middle and at the top during 36 days of composting. At an external temperature of 50°C, E. coli O157:H7 was inactivated rapidly (by ca. 4.9 log10 CFU/g at the top of the bioreactor, by 4.0 log10 CFU/g near the middle, and by 5.9 log10 CFU/g near the bottom) within 24 h of composting. When inoculated at an initial level of ca. 107 CFU/g, E. coli O157:H7 survived for 7 days but not for 14 days at all three sampling locations, as indicated by either direct plating or enrichment culture. At the top of the bioreactor a relatively constant moisture content of 60% was maintained, whereas the moisture content near the bottom decreased steadily to 37 to 45% over 14 days of composting. The pH of the composting mixture decreased to ca. 6 within 1 to 3 days and subsequently increased to 8 to 9. Results obtained in this study indicate that large populations (104 to 107 CFU/g) of E. coli O157:H7 survived for 36 days during composting in a bioreactor at an external temperature of 21°C but were inactivated to undetectable levels after 7 to 14 days when the external temperature of the bioreactor was 50°C. Hence, manure contaminated with large populations (e.g., 107 CFU/g) of E. coli O157:H7 should be composted for more than 1 week, and preferably for 2 weeks, when held at a minimum temperature of 50°C.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

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