scholarly journals Gambaran Histopatologi Pankreas Tikus Putih (Rattus norvegicus) Pasca Perlakuan Iskemia-Reperfusi Ginjal

2019 ◽  
Vol 13 (1) ◽  
pp. 58
Author(s):  
Elva Rosiana ◽  
Wiwit Ade Fidiawati ◽  
Darmawi Darmawi

Ischemic-reperfusion injury is defined by a condition of hypoperfusion in the spesific organ followed by reperfusion(reoxygenation) inducing tissue damage. Tissue damage produces reactive oxygen species (ROS) leading to oxidativestress condition. Oxidative stress mediate the lipid peroxidation reactions, harm the cell and finally facilitate the celldeath. The aim of this study was to evaluate the histopathologic feature of white rat’s pancreas post renal ischemiareperfusion.This study was an experimental laboratory research with post-test-only control group design including 20male white rats. Rats were divided into 5 groups, control, treatment 1 (45 minutes ischemia), treatment 2 (45 minutesischemia followed by 1 hour reperfusion), treatment 3 (45 minutes ischemia followed by 2 hours reperfusion) andtreatment 4 (45 minutes ischemia followed by24 hours reperfusion). We found the change of the histopathologicalcharacteristics of white rat’s pancreas in term of edema, leukocyte infiltration and vacuolization.

2018 ◽  
Vol 11 (2) ◽  
pp. 166
Author(s):  
Rizky Prihandari ◽  
Lailatul Muniroh

Cigarette smoke contains many hazardous substances and free radical which affect respiratory system. Watermelon “Sugar Baby” juice contain lycopene as antiinfl ammation. The aim of this research was to analyze the effect of watermelon as antiinfl ammation toward neutrophils count in wistar strain male rats exposed by  cigarette smoke. This study was an experimental laboratory research with pre and post test control group design. Sample consisted of 24 wistar strain male rats with ±150-230 grams weight and 3 months old which were divided into four groups. Control group was given placebo (aquadest) and treatment groups were given w atermelon juice with terraced quantity (1.5 ml, 2.5 ml, and 3.5 ml). Every group was given two sticks of cigarette smoked per day during two weeks then smoked with treatment during one week. Neutrophil from peripheral smear was observed by manual method and statistically analyzed using Anova Mixed Design and continued with One Way Anova. The result showed signifi cant increased of  neutrophil at second observation on control group (p=0.002) and treatment groups (p=0.000). Signifi cance decreased also found in the treatment groups’s at third observation p=0.000. Signifi cant between each group p= 0.017. There was signifi cant different between control and treatment groups but there was no signifi cant different between each treatment groups. This study concluded that there was decreased of  neutrophil in wistar strain male rats which were given w atermelon juice 1.5 ml, 2.5 ml, and 3.5 ml during infl ammation. 3.5 ml w atermelon juice which were given showed better result as antiinfl ammation..


2017 ◽  
Vol 52 (1) ◽  
pp. 62
Author(s):  
Ardy Hamonangan Naibaho ◽  
Arifa Mustika ◽  
Anny Setijo Rahaju

Singawalang leaf is used as a traditional medicine by the communities suffering from pneumonia due to TB disease. However, experiments to explain it’s toxicity are few. Therefore, this study was conducted to disclose it’s toxicity. This was an experimental laboratory study using post–test only control group design. The sampling method was simple random sampling. The animals were randomly divided into 4 (four) groups, each group consisted of 8 (eight) mice, the control group (P0) received distilled water as a solvent material. Treatment group 1 (P1) obtained ethanol extract of singawalang (Petiveria alliaceae) in low doses of 90 mg/kgbw. Treatment group 2 (P2) obtained ethanol extract of singawalang (Petiveria alliaceae) in medium doses of 180 mg/kgbw. Treatment group 3 (P3) obtained ethanol extract of singawalang (Petiveria alliaceae) in high doses of 360 mg/kgbw. Th extracts were given with sonde once a day for 30 days. Results showed that the leaf ethanol extract singawalang (Petiveria alliaceae) does not induce sub-acute necrosis effects on kidney of male white mice (Mus musculus) in doses 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw.


2014 ◽  
Vol 3 (3) ◽  
Author(s):  
Fauzan Arisyi Koto ◽  
Husnil Kadri ◽  
Zelly Dia Rofinda

AbstrakKopi merupakan salah satu minuman yang paling banyak dikonsumsi di dunia. Banyak studi yang meneliti efek konsumsi kopi terhadap berbagai kondisi medis tertentu. Salah satu efek dari kopi yang masih menjadi kontroversi adalah efek terhadap penurunan kadar asam urat. Kandungan polifenol dalam kopi diduga dapat menghambat kerja xantin oksidase sehingga menurunkan kadar asam urat. Tujuan penelitian ini adalah untuk menentukan pengaruh pemberian kopi oral terhadap kadar asam urat serum pada tikus wistar. Ini adalah penelitian eksperimental dengan rancangan post test only control group design. Sampel penelitian adalah 24 ekor tikus putih jantan (Rattus novergicus) yang dibagi menjadi 4 kelompok yaitu kelompok kontrol, perlakuan 1, perlakuan 2 dan perlakuan 3. Kontrol hanya diberi diet standar tanpa kopi, perlakuan 1diberikan diet kopi dosis rendah setara 3 cangkir kopi (0,39 mg/3 ml), perlakuan 2 diberikan diet kopi dosis sedang setara 6 cangkir kopi (0,78 mg/ 3ml), perlakuan 3 diberikan diet kopi dosis tinggi setara 10 cangkir kopi (1,3 mg/ 3ml) selama 4 minggu (28 hari). Pengukuran kadar asam urat serum menggunakan spektofotometer. Hasil penelitian didapatkan rerata kadar asam urat serum kontrol (2,26+0,16 mg/dl), perlakuan 1 (2,24+0,89 mg/dl), perlakuan 2 (1,00+0,33 mg/dl), perlakuan 3 (1,96+0,43 mg/dl). Uji analisis one way Anova dan Post hoc menunjukkan bahwa perbedaan yang bermakna hanya terdapat pada perbandingan kelompok kontrol dengan kelompok perlakuan 2 dan antara kelompok perlakuan 1 dengan kelompok perlakuan 2 (p<0,05). Kesimpulan dari penelitian ini adalah terdapat penurunan kadar asam urat serum setelah pemberian kopi dan terdapat perbedaan bermakna kadar asam urat antara kelompok kontrol dan perlakuan 1 dengan kelompok perlakuan 2.Kata kunci: kopi, polifenol, asam urat serumAbstractCoffee is one of the most frequently consumed beverages in the world. Many studies have examined the effect of coffee consumption on a wide range of specific medical condition. One of the effects of coffee that still a controversy is the effect of the reduction in uric acid levels because the polyphenol in coffee could be expected to inhibit xanthine oxidase to lower uric acid level. The objective of this study was to determine the effect of coffee on uric acid levels Wistar strain of rats (Rattus novergicus). This was a experimental study with posttest only control group design. Samples were 24 male white rats strain Wistar divided into 4 groups: control, treatment 1, treatment 2, and treatment 3. Control was not treat with coffee, treatment 1 were given low dose of coffee equivalent to 3 cups (0,39 mg/3 ml), treatment 2 were given moderate dose of coffee equivalent to 6 cups (0,78 mg/ 3ml), treatment 3 were given high dose of coffee equivalent to 10 cups (1,3 mg/ 3ml) for 4 weeks (28 days). Serum uric acid levels measured with sphectrophotometer The result showed the mean of uric acid levels control (2,26+0,16 mg/dl), treatment 1 (2,24+0,89 mg/dl), treatment 2 (1,00+0,33 mg/dl), treatment 3 (1,96+0,43 mg/dl). One way Anova dan Post hoc tests analysis showed significant differences of uric acid levels only between control and treatment 2 group and between treatment 1 group and treatment 2 group (p<0,05). The conclusion of this study is reduction of uric acid levels after coffee consumption and the significant differences in uric acid levels between control/treatment 1 group with treatment 2 group.Keywords: coffee, polyphenol, uric acid levels


2018 ◽  
Vol 6 (1) ◽  
pp. 36-44
Author(s):  
Rizky Rafiqoh Afdin ◽  
Fairuz Quzwain

ABSTRACT Background: Among the various medicinal plants, black cumin (Nigella sativa) appears as an herb with a historical and religious background due to a lot of research. One of the most important effects on Nigella sativa is the hepatoprotective that has been shed in various studies. Thymoquinone which is the content of Nigella sativa has the ability to inhibit lipid peroxidation. Thymoquinone can reduce oxidative stress and increase antioxidants in the body. The objectives of this study were to overcome the effects hepatoprotector of black cumin extract (Nigella sativa) on the damage of hepatic of male white rat (Rattus norvegicus) sparague dawley strain induced by ethanol. Methodology: It is a kind of experimental laboratory research used that uses random controlled design method with the pattern of post test-only control group design. The subject of this research is 25 adult male white rats (Rattus norvegicus) Sparague Dawley strain aged 3-4 months, weight 150-300 grams, that are divided in to five groups. Group one (normal control) is given aquades dose 0,01 ml/grBB/day, group two (pathologic control) is given ethanol with dose o,o1 ml/grBB/day, group three is given 25% concentration of black cumin extract and 50% ethanol, group four is given 37,5% concentration of black cumin extract and 50% ethanol, and group five is given 50% concentration of black cumin extract and 50% ethanol. After 14th days, the treatment was stopped and the rats got dislocation cervical, and then laparotomy for made histopathology preparation. After getting observed, the data that is gotten is analyzed with Kruskal-Wallis test and then Mann-Whitney test. Result: The result of Kruskal-Wallis test showed that there was an effect of black cumin extract (Nigella sativa) on the damage of hepatic of male white rat (Rattus norvegicus) sparague dawley strain induced by ethanol with p = 0,001 (p <0,05) . Mann Whitney test show there is a means difference between group I (normal control) with group II (pathologic control), group III (dose I), group IV (dose II) and group V (dose III). Conclusion: There is a hepatoprotector effect of black cumin (Nigella sativa) extract to liver’s damage of white rats (Rattus norvegicus) sparague dawley strain induced by ethanol. Key words: Black cumin extract, ethanol, fatty degeneration of liver rats.   ABSTRAK Latar Belakang: Di antara berbagai tanaman obat, jintan hitam (Nigella sativa) muncul sebagai ramuan dengan latar belakang sejarah dan agama karena banyak penelitian mengungkapkan potensi farmakologis dari tanaman tesebut. Salah satu efek yang paling penting pada Nigella sativa adalah hepatoprotektif yang ditelah dijelaskan dalam berbagai penelitian. Thymoquinone yang merupakan kandungan jintan hitam memiliki kemampuan untuk menghambat peroksidasi lipid. Thymoquinone dapat mengurangi stress oksidatif dan meningkatkan pertahanan antioksidan dalam tubuh.  Tujuan penelitian ini untuk meneliti efek hepatoprotektor jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur Sprague Dawley yang diinduksi etanol. Metode: Jenis penelitian ini adalah eksperimental laboratorik menggunakan metode rancangan acak terkontrol dengan pola post test-only control group design. Subjek penelitian adalah 25 ekor tikus putih (Rattus norvegicus) jantan galur sparague dawley berumur 3-4 bulan, berat badan 150-300 gram, yang dibagi menjadi 5 kelompok. Kelompok I (kontrol normal) diberi aquades dosis 0,01 ml/grBB/hari, kelompok II (kontrol patologis) diberi etanol 50% dosis 0,01 ml/grBB/hari, kelompok III diberi ekstrak jintan hitam konsentrasi 25% dan etanol 50%, kelompok IV diberi ekstrak jintan hitam konsentrasi 37,5% dan etanol 50%, serta kelompok V diberi ekstrak jintan hitam konsentrasi 50% dan etanol 50%. Setelah hari ke-14, perlakuan dihentikan, dilakukan dislokasi pada leher tikus kemudian dilakukan laparotomi untuk pengambilan hepar tikus untuk dibuat preparat histopatologis. Setelah diamati, data dianalisis dengan uji Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney. Hasil: Hasil uji Kruskal-Wallis menunjukkan terdapat pengaruh ekstrak jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur sparague dawley yang diinduksi etanol, dengan nilai p = 0,001 (p<0,05). Uji Mann Whitney menunjukkan perbandingan yang bermakna didapatkan pada kelompok I (kontrol normal) dengan kelompok II (kontrol patologis), kelompok III (dosis I), kelompok IV (dosis II) dan kelompok V (dosis III). Kesimpulan: Terdapat efek hepatoprotektor ekstrak jintan hitam (Nigella sativa) terhadap kerusakan hepar tikus putih (Rattus norvegicus) jantan galur Sprague Dawley yang diinduksi etanol.   Kata kunci: ekstrak jintan hitam, etanol, degenerasi lemak hepar tikus.


2019 ◽  
Vol 3 (4) ◽  
pp. 231 ◽  
Author(s):  
Mohamad Riza ◽  
Andina Putri A

Objective: to compare the effect of administration of energy drinks and coffee on creatinine levels in rats.Methods: an experimental laboratory research using the post test only control group design. The study used 18 male white Sprague Dawley divided into three groups to receive one of the following treatments: 2 cc distilled water,0.072 g /2cc energy drinks and 0.9 g/cc of coffee for 14 days. The creatinine level was evaluated. Data were analyzed by One Way ANOVA test.Results: The mean creatinine level in the aquades group, energy drinks, coffee group was 0.708 mg/dl, 2.05 mg/dl 1.861 mg/dl, respectively. Post Hoc test the LSD test showed a significant difference among these groups (p <0.05).Conclusion: There was a significant different effect between 0.072 g / 2cc energy drinks and 0.9 g/cc coffee on creatinine levels in rat.International Journal of Human and Health Sciences Vol. 03 No. 04 October’19 Page : 231-234


2019 ◽  
Vol 6 (1) ◽  
pp. 106
Author(s):  
Sutomo Sutomo ◽  
Muhammad Rafi ◽  
Arnida Arnida

ABSTRAK             Kasturi (Mangifera casturi Kosterm) merupakan tumbuhan khas Kalimantan Selatan yang memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk menentukan gambaran makroskopis (warna dan volume) organ hati serta perubahan histopatologi sel hati (degenerasi parenkimatosa, degenerasi hidropis, dan nekrosis) pada tikus setelah diberikan fraksi etil asetat buah M. casturi secara peroral. Penelitian ini berupa penelitian eksperimental dengan rancangan post test only control group design. Hewan dibagi menjadi 5 kelompok yaitu kelompok kontrol (Na-CMC 0,5%) dan kelompok perlakuan (5, 50, 300, dan 2000 mg/kgBB). Setiap kelompok terdiri dari 5 tikus putih jantan yang diberikan perlakuan 1 kali selama 14 hari, setelah itu tikus dibedah dan diambil organ hati untuk diamati. Hasil analisis Kruskal Wallis menunjukkan bahwa pemberian fraksi etil asetat buah M. casturi pada gambaran makroskopis (volume) hati tidak berbeda bermakna antara kelompok kontrol dan perlakuan (p > 0,05). Hasil analisis One-Way ANOVA pada gambaran mikroskopis hati menunjukkan bahwa kelompok kontrol dan perlakuan tidak terdapat perbedaan bermakna. Kata kunci : Mangifera casturi, fraksi etil asetat, toksisitas, degenerasi, nekrosis  ABSTRACT Kasturi (Mangifera casturi Kosterm.) is the typical plants of South Kalimantan that contains antioxidant activity. The aim of this study was to determine the macroscopical representative (color and volume) of rats liver and the microscopical representative of hepar cells (parenchymatous degeneration, hydropic degeneration, and necrosis) after oral administration of ethyl acetate fraction of M. casturi fruit. This study was an experimental study with post-test only control group design. Test animals were divided into 5 groups consisted of a control group (CMC Na 0,5%), and treatment groups (5, 50, 300, and 2000 mg/kg bw). Each group consisted of 5 the male white rats which were treated once for 14 days. And then the rats were dissected, the liver were taken out to make preparations of histopathological examination and observation. The results of Kruskal Wallis showed that ethyl acetate fraction of M. casturi fruit had no difference on macroscopical representation of the liver. Keywords : Mangifera casturi, ethyl acetate fraction, toxicity, degeneration, necrosis


2017 ◽  
Vol 3 (1) ◽  
pp. 447
Author(s):  
Iyay Robia Khoerudin ◽  
Neneng Titin ◽  
Eki Kiyamudin

Penelitian ini bertujuan untuk mengetahui kemampuan menulis surat dinas siswa kelas VIII di SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka, mengetahui kemampuan menulis surat dinas dengan menggunakan model pembelajaran STAD (Student Teams-Achievment Divisions) siswa kelas VIII di SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka, serta menjelaskan efektifitas model pembelajaran STAD (Student Teams-Achievment Divisions) untuk meningkatkan  pengajaran menulis surat dinas siswa Kelas VIII di SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka.Penelitian ini menggunakan pendekatan kuantitatif dan kualitatif dengan metode eksperimen Pre test-Post test Control Group Design, populasinya yaitu SMP Negeri Se-Kecamatan Panyingkiran Kabupaten Majalengka tahun ajaran 2013/2014, sedangkan  sampelnya ditentukan melalui teknik simple random sampling yaitu kelas VIII G SMP Negeri 2 Panyingkiran sebagai kelas eksperimen dan kelas VIII B SMP Negeri 1 Panyingkiran sebagai kelas kontrol. Instrumen pengumpulan data yang digunakan tes tertulis tes awal dan tes akhir, kuesioner, lembar observasi guru dan siswa, setelah data diperoleh maka dilakukan analisis data. Data kuesioner dan observasi dianalisis dalam bentuk uraian sedangkan data tes dianalisis dengan statistik dan di deskripsikan.Berdasarkan perhitungan statistik program SPSS 16.0 diperoleh hasil uji t pretes pada kedua kelas tersebut memiliki nilai t=5.429 derajat kebebasan (df)=n-1=38 nilai probability (sig 2-tailed) sebesar 0,000.  Hal ini berarti t hitung <0,005. Artinya 0,000<0,05, pada uji t ini bahwasanya pretes pada kedua kelas terdapat perbedaan yang signifikan antara kelas eksperimen dengan kelas kontrol.  Apabila ditinjau dari segi rata-rata pretes eksperimen lebih efektif daripada kelas kontrol. Hal ini terbukti dari rata-ratanya pretes kelas eksperimen 64,8 kelas kontrol 62,3 selisih rata-rata 2,5. Sedangkan rata-rata postes kelas eksperimen 81,2 dan kelas kontrol 69 terjadi selisih postes 12,2.


2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Kadir Kadir ◽  
Munawir Sadzali

Tujuan dari penelitian ini adalah menganalisis pengaruh model pembelajaran kooperatif tipe Number Head Together (NHT) terhadap keterampilan problem posing matematis siswa. Penelitian ini dilakukan di SD Al- Zahra Indonesia, untuk tahun akademik 2014/2015. Metode yang digunakan dalam penelitian ini adalah metode kuasi eksperimen dengan Post-test Only Control Group Design, melibatkan 60 siswa sebagai sampel. Untuk menentukan sampel digunakan teknik cluster random sampling. Pengambilan data menggunakan instrumen seperti tes esai tertulis. Keterampilan problem posing matematis siswa yang mengajar dengan model pembelajaran kooperatif tipe Number Head Together (NHT) lebih tinggi daripada keterampilan problem posing matematis siswa yang diajarkan dengan model pembelajaran klasik (t hitung = 2,01 table t tabel = 2,00 ). Persentase jawaban siswa dari problem posing berdasarkan gambar, tabel dan diagram yang diajarkan dengan model pembelajaran kooperatif tipe Number Head Together (NHT) lebih tinggi daripada siswa yang mengajar dengan model pembelajaran klasik. Kesimpulan hasil penelitian ini bahwa pembelajaran matematika Pecahan dengan model pembelajaran kooperatif tipe Number Head Together (NHT) memiliki pengaruh yang signifikan terhadap keterampilan problem posing matematika siswa.


2018 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
A.P. Adhiyanti ◽  
A.A.I.N Marhaeni ◽  
I.P.I Kusuma

  This study aimed to investigate the effect of anonymous peer assessment on students’ writing ability. Quantitative approach was used in this study. Post-test only control group design was the design used in this experimental study. The post-test only control group design was designed to discover the purpose of this study which was to investigate the significant effect on writing ability between students who obtain feedback from anonymous peer assessment and students who obtain feedback from conventional assessment. The study was conducted at SMK PGRI 1 Singaraja. The eleventh grade students in Academic Year 2017/2018 were chosen as the population. The samples were 44 students from XI AP and XI UPW. The instruments used in this study were lesson plan, anonymous peer checklist, writing scoring rubric, and writing test. Writing scoring rubric and writing test were used to obtain the data through post-test while lesson plan and anonymous peer assessment were used during the treatment. The data then was analyzed by using SPSS 16. The results of the analysis show that (1) the mean score of the experimental group was 77.55 while the mean score of the control group was 68.77. (2) The t-observe was 3.892 and it exceeded the t-critical value which was 2.018. Therefore, based on the aforementioned results, it can be concluded that there was a significant difference on writing ability between the eleventh grade students who obtained feedback from anonymous peer assessment and those who obtained feedback from conventional assessment at SMK PGRI 1 Singaraja in academic year 2017/2018. 


2013 ◽  
Vol 19 (1) ◽  
pp. 52
Author(s):  
Adi Suarman Situmorang

Abstract Tujuan penelitian bertujuan untuk mengetahui peningkatan kemampuan kreativitas matematika siswa yang diajar dengan model pencapaian konsep lebih tinggi dari siswa yang diajar dengan pembelajaran konvensional. Mengetahui interaksi antara model pembelajaran pencapaian konsep dengan tingkat kemampuan matematika siswa terhadap peningkatan kemampuan kreativitas matematika siswa. Penelitian ini dilaksanakan di SMA Negeri 5 Medan dengan jumlah sampel sebanyak 80 siswa dari 364 siswa SMA kelas X melalui teknik random sampling, Penelitian ini merupakan suatu studi eksperimen dengan desain penelitian pre-test-post-test control group design. Data diperoleh melalui nilai semester untuk kemampuan awal matematis (KAM), tes kemampuan pemahaman matematis, tes kemampuan kreativitas matematis. Data dianalisis dengan uji ANAVA dua jalur. Hasil analisis data menunjukkan bahwa rata-rata tes kemampuan kreativitas eksperimen dan kontrol adalah 13,3 dan 7,58 dengan p-value (2-tailed) adalah 0, dengan 0 < α = 0,05 maka terdapat perbedaan kemampuan kreativitas matematik siswa yang diajarkan dengan Model Pencapaian Konsep (MPK) dan Pendekatan Pembelajaran Konvensional, nilai signifikan sebesar 0,732, karena 0,732 > 0,05 maka tidak ada interaksi antara pendekatan pembelajaran dengan kemampuan awal siswa terhadap perbedaan kemampuan kreativitas matematik siswa. .


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