scholarly journals Effectiveness of Soursop Leaf Extract to Decrease Malondialdehyde Level

2020 ◽  
Author(s):  
Sarah Lorenza Caverina ◽  
◽  
Retno Yulianti ◽  
Andri Pramesyanti ◽  
◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Free Radicals ◽  
Effective Dose ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Group I ◽  
Significant Difference

ABSTRACT Background: In diabetes mellitus, hyperglycemia causes oxidative stress that enhances the production of free radicals. The presence of higher levels of malondialdehyde (MDA) indicates the rise of free radicals. On the basis of its role as an antioxidant that binds free radicals and reduces the level of malondialdehyde (MDA). Soursop leaf can act as an antidiabetic agent. This study aimed to examine the efficacy of soursop leaf extract (Annona muricata L.) to decrease the level of MDA level in alloxan-induced diabetic rats (Rattus norvegicus). Subjects and Method: This was an experiment study using pre- and pro-test with control group design. Total of 30 rats were selected for this study and divided into five groups of treatment i.e. group I was given distilled water and standard feed (Negative Control/KI), group II was given vitamin E 150 IU/kgBW/day (Positive Control/KII), group III was given soursop leaf extract 75 mg/kgBW/day (KIII), group IV was given soursop leaf extract 150 mg/kgBW/day (KIV), and group V was given soursop leaf extract 300 mg/kgBW/day (KV). Each group consisted of 5 rats and was given soursop leaf extract for 21 days after being induced by alloxan and high-fat diet. The data were analyzed using Mann-Whitney test. Results: There was a significant difference in KI group compared to KII, KIII, KIV, and KV groups. Soursop leaf extract of 150 mg/kgBW/day was the most effective dose to reduce MDA level in pancreatic rat. Conclusion: Soursop leaf extract of 150 mg/kgBW/day is the most effective dose on pancreatic MDA level reduction. Keywords: diabetes mellitus, soursop leaf extract, MDA, free radical Correspondence: Retno Yulianti. Department of Anatomical Pathology, Faculty of Medicine Universitas Pembangunan Nasional “Veteran” Jakarta. Jl. Fatmawati Hospital, Pondok Labu, South Jakarta. Email: [email protected] DOI: https://doi.org/10.26911/the7thicph.05.07

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals EFFECTIVENESS OF LEAF EXTRACT REDUCE INFLAMMATION REACTION IN HYPERCHOLESTEROLEMIA RATS

2017 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Puguh Santoso ◽  
Ni Nyoman Wahyu Udayani ◽  
I Nyoman Gede Tri Sutrisna ◽  
Ketut Agus Adrianta
Keyword(s):  
Free Radicals ◽  
Leaf Extract ◽  
Oxidized Ldl ◽  
Oxidative Modification ◽  
Blood Cholesterol ◽  
Ldl Oxidation ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Group I

Abstract High blood cholesterol is often called hypercholesterolemia is a risk factor for the emergence of pathological conditions such as heart and blood vessel disease. Hypercholesterolemia has an important role in the occurrence of damage to the endothelial cells is mainly caused by oxidized LDL. Oxidation of LDL triggers the formation of TNF - ?. Leaves messengers that allegedly contains flavonoids can improve the situation of hypercholesterolemia through the barriers specifically the expression of TNF - ? increased due to hypercholesterolemia. Plants messengers known to contain alkaloids, flavonoids, tannins, saponins, polyphenols, calcium oxalate, fats, and essential oil. Flavonoids which has the ability to bind the atom to form free radicals not to excess free radicals, thereby inhibiting the oxidative modification of LDL become ox-LDL so it will not be formed atherosclerosis. This study uses the Randomize pattern Pre and Post Test Control Group Design, using white rats (Rattusnovergicus) with Wistar strain aged 3-4 months, weighing 175-200 grams. Divided into four groups: Group I as a control with placebo, Group II treatment using extracts of ethanol leaves a messenger at a concentration of 10%, Group III treatments using extracts of ethanol leaves a messenger at a concentration of 20%, Group IV is to use the extract ethanol leaves errand at a concentration of 30%. It can be concluded at 4 dose group 30% messengers leaf extract significantly different, p <0.05, so it can be said that the provision of effective messengers extract at a dose of 30%.

scholarly journals THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

2018 ◽  
Vol 11 (12) ◽  
pp. 411
Author(s):  
Ameta Primasari ◽  
Minasari Nasution ◽  
Nurul Hidayati Arbi ◽  
Dini Permata Sari ◽  
Mohammad Basyuni
Keyword(s):  
Leaf Extract ◽  
Brain Heart Infusion ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals EFEKTIVITAS EKSTRAK DAUN JERUK PURUT (Citrus hystrix) SEBAGAI INSEKTISIDA ALAMI TERHADAP KECOAK AMERIKA (Periplaneta americana) DENGAN METODE SEMPROT

2020 ◽  
Vol 8 (1) ◽  
pp. 8-14
Author(s):  
Hanina Hanina ◽  
Sarah Madeleyne Baringbing
Keyword(s):  
Leaf Extract ◽  
Periplaneta Americana ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Observation Time ◽  
American Cockroach ◽  
Control Group ◽  
Significant Difference ◽  
Post Test

ABSTRACT Cockroach control can be done in various ways one of which is spraying insecticide. Natural insecticides are less dangerous than conventional insecticides. This study aimed to determined the effectiveness of kaffir lime leaf extract (Citrus hystrix) as a natural insecticide against cockroach americana (Periplaneta americana) with spray method. This research was an experimental study with a post-test only control group designed design. The sample in this study was 60 cockroaches, the sample was divided into 6 groups: negative control (aquades), positive control (transflutrin 0.06%) and and 4 test groups with concentrations of kaffir lime leaf extract (20%, 40%, 60% , and 80%) each group consisted of 10 cockroaches. Phytochemical tests were carried out to determine the content of secondary metabolites in extracts of kaffir lime leaves. Positive phytochemical test results containing flavonoids, alkaloids and essential oils. Based on probit analysis, it is known that the LC50 value = 58.5%; LC95 = 85.1%. The time of death of LT50 is fastest at a concentration of 60% (3,036 hours) and LT95 is the fastest at a concentration of 80% (5,960 hours). Anova test analysis results showed that the positive control group had no significant difference with kaffir lime leaf extract concentration of 80% Kaffir lime leaf extract has an insecticide effect, the higher the concentration of kaffir lime leaf extract, the more number of dead cockroaches and the longer the observation time, the number of cockroaches death will also increase. Keywords: American cockroach, kaffir lime leaf extract, cockroach death   ABSTRAK Pengendalian kecoa dapat dilakukan dengan berbagai cara salah satunya penyemprotan insektisida. Insektisida alami kurang berbahaya dibanding insektisida konvensional. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak daun jeruk purut (Citrus hystrix) sebagai insektisida alami terhadap kecoak americana (Periplaneta americana) dengan metode semprot. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan post-test only control group designed. Sampel dalam penelitian ini adalah 60 ekor kecoak, sampel dibagi menjadi 6 kelompok yaitu kontrol negatif (aquades), kontrol positif (transflutrin 0,06%) dan dan 4 kelompok uji dengan konsentrasi ekstrak daun jeruk purut (20%, 40%, 60%, dan 80%) masing-masing kelompok terdiri dari 10 ekor kecoak. Uji fitokimia dilakukan untuk mengetahui kandungan senyawa metabolit sekunder pada ekstrak daun jeruk purut. Hasil uji fitokimia positif mengandung flavonoid, alkaloid dan minyak atsiri. Berdasarkan analisis probit, diketahui nilai LC50 = 58,5%; LC95 = 85,1%. Waktu kematian LT50 paling cepat pada konsentrasi 60% (3,036 jam) dan LT95 paling cepat pada konsentrasi 80% (5,960 jam). Hasil analisis uji anova menunjukkan bahwa kelompok kontrol positif tidak terdapat perbedaan yang signifikan dengan ekstrak daun jeruk purut konsentrasi 80% Ekstrak daun jeruk purut memiliki efek insektisida, semakin tinggi konsentrasi ekstrak daun jeruk purut maka jumlah kecoak yang mati semakin banyak dan semakin lama waktu pengamatan, maka jumlah kecoak yang mati juga akan semakin banyak. Kata kunci : kecoak amerika, ekstrak daun jeruk purut, kematian kecoak

scholarly journals Water Hyacinth (Eichhorniacrassipes) Ethanolic Extract Anti Platelet Activity

2021 ◽  
pp. 969-978
Author(s):  
Perdina Nursidika
Keyword(s):  
Cardiovascular Diseases ◽  
Blood Vessel ◽  
Effective Dose ◽  
Water Hyacinth ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Control Group ◽  
Standard Group ◽  
Group I ◽  
Significant Difference

Cardiovascular diseases (CVDs) are a group of disorders of the heart and blood vessels. CVDs are responsible for 37% of deaths in Indonesia. Thrombosis is the main factor causing blood vessel clost in CVDs. Blood vessel clots then affect narrowed blood vessel. This causes strokes. The aim of this study is to determine the antithrombotic effect of water hyacinth ethanolic extract, the effective dose, and the potential to become anticoagulant agent. The water hyacinth was extracted by ethanol 96%. The extract was tested on five groups of mice, each group containing five mice. Each group was tested for fourteen days. Group I and II are fed by extract suspension with varied dose 1 mg/BW and 2 mg/BW. Group III is fed by warfarin as standard. Group IV and V are the positive and negative group, the mice were fed by NaCMC. On the 7th and 14th days, prothrombin time (PT) and activated partial thromboplastin time (aPTT) were conducted. The plasma of group 1-IV mixed with adenosine diphosphate (ADP) as inducer, except plasma of group V used for negative control, did not mixed with ADP. Data analysed by ANOVA and LSD to obtain effective dose. The results showed the difference between PTT and aPTT among the tested groups. Group I and II showed prolonged PT which is 22 and 32 seconds respectively. The control positive showed PT 9.48 seconds on day 7th and 8.22 seconds on day 14th. LSD analysis showed that there is the significant difference between positive control and the other groups. It showed platelet aggregation performed due to the addition of ADP as inducer. There was a significant difference between dose 2 and the negative control group, and there is no significant difference of dose 1. In conclusion, water hyacinth has the antithrombotic activity, the effective dose is 1 mg/BW, and it potential to further developed as anticoagulant agent.   Keywords: antiplatelet, cardiovascular diseases, water hyacinth.

scholarly journals Efektivitas perasan daun seledri (Apium graveolens Linn.) sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastikThe effectiveness of celery leaf juice (Apium graveolens Linn.) as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base

2020 ◽  
Vol 32 (3) ◽  
pp. 184
Author(s):  
Dhilan Purna Aji ◽  
Achmad Gunadi ◽  
Tantin Ermawati
Keyword(s):  
Candida Albicans ◽  
Positive Control ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Control Group Design ◽  
Denture Base ◽  
Significant Difference ◽  
Post Test ◽  
Leaf Juice

Pendahuluan: Basis gigi tiruan nilon termoplastik merupakan alternatif pengganti basis gigi tiruan resin akrilik. Pembersihan gigi tiruan diperlukan untuk menjaga basis gigi tiruan agar tetap bersih dari kontaminasi mikroorganisme. Daun seledri merupakan bahan alam yang memiliki kandungan flavonoid, saponin, tanin, dan minyak atsiri yang sudah terbukti bersifat antijamur. Penelitian sebelumnya hanya melihat zona hambat saja. Tujuan penelitian adalah menganalisis efektivitas perasan daun seledri sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastik. Metode: Eksperimental laboratoris dengan rancangan penelitian post-test only control group design. Sampel nilon termoplastik berbentuk cakram dengan diameter 10 mm dan tebal 2 mm  sebanyak 25 dibagi menjadi 5 kelompok. Sampel dikontaminasi dengan C. albicans setelah direndam dalam kontrol negatif (akuades) selama 6 jam, kontrol positif (tablet effervescent) selama 15 menit, dan perasan daun seledri konsentrasi 25, 50, dan 100% selama 6 jam. Sampel selanjutnya diletakkan dalam media sabouraud dextrose broth (SDB). Pengujian efektivitas perasan daun seledri dilakukan dengan menentukan nilai absorbansi kekeruhan media SDB menggunakan spektrofotometer. Data dianalisis menggunakan one-way ANOVA dan Least Significant Difference (LSD). Hasil: Nilai konsentrasi C. albicans pada kelompok kontrol negatif mendapatkan hasil 0,380 x 108 CFU/mL, kontrol positif mendapatkan hasil 0,310 x 108 CFU/mL, perasan daun seledri konsentrasi 25% mendapatkan hasil 0,243 x 108 CFU/mL, konsentrasi 50% mendapatkan hasil 0,160 x 108 CFU/mL, dan konsentrasi 100% mendapatkan hasil 0,236 x 108 CFU/mL. Simpulan: Perasan daun seledri efektif sebagai pembersih gigi tiruan untuk menghambat  pertumbuhan C. albicans, dengan konsentrasi yang efektif sebesar 50%.Kata kunci: Nilon termoplastik, perasan daun seledri, Candida albicans. ABSTRACTIntroduction: Thermoplastic nylon denture base is an alternative to the acrylic resin denture base. Denture cleaning is needed to keep the denture base clean from contamination by microorganisms. Celery leaves are natural ingredients that contain flavonoids, saponins, tannins, and essential oils that have been proven to have antifungal properties. Previous research only observed the inhibitory zone. This research was aimed to analyse the effectiveness of celery leaf juice as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base. Methods: Experimental laboratory with a post-test only control group design was conducted towards 25 disc-shaped thermoplastic nylon samples with a diameter of 10 mm and a thickness of 2 mm, and divided into five groups. Samples were contaminated with C. albicans after immersion in the negative control (aquadest) for 6 hours, positive control (effervescent tablets) for 15 minutes, and celery leaf juice with the concentrations of 25, 50, and 100% for 6 hours. The sample was then placed in the Sabouraud-dextrose broth (SDB) medium. The effectiveness of celery leaf juice was tested by determining the absorbance value of SDB media turbidity using a spectrophotometer. Data were analysed using one-way ANOVA and Least Significant Difference (LSD). Results: The concentration value of C. albicans in the negative control group was 0.380 x 108 CFU / mL, the positive control was 0.310 x 108 CFU / mL, the juice of celery leaves with the concentration of 25% was 0.243 x 108 CFU / mL, the concentration of 50% was 0.160 x 108 CFU / mL, and the concentration of 100% was 0.236 x 108 CFU / mL. Conclusion: Celery leaf juice is effective as a denture cleaner to inhibit the growth of C. albicans, with an effective concentration of 50%.Keywords: Thermoplastic nylon, celery leaf juice, Candida albicans.

scholarly journals Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

2011 ◽  
Vol 9 (2) ◽  
pp. 33-37
Author(s):  
MUHAMMAD ARIF NUR SYAHID ◽  
CR. SITI UTARI ◽  
SUTARMIADJI DJUMARGA
Keyword(s):  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mimosa Pudica ◽  
Control Group Design ◽  
Significance Level ◽  
Death Time ◽  
Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

scholarly journals The effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats

2012 ◽  
Vol 10 (1) ◽  
pp. 23-27
Author(s):  
JUDO YUSTANTO KAHONO ◽  
KISRINI KISRINI ◽  
YUL MARIYAH
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Total Period ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Kahono JY, Kisrini, Mariyah Y. 2012. The effect of meniran herbs (Phyllantus niruri) extract to blood triglyceride level in rats. Biofarmasi 10: 23-27. The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1st and 21st, blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.

Daya Antihiperglikemia Yoghurt Pada Mencit Putih Jantan

2019 ◽  
Vol 4 (2) ◽  
pp. 72
Author(s):  
Irene Puspa Dewi ◽  
Roza Septriani ◽  
Verawaty Verawaty ◽  
Dwi Mulyani
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Percentage Increase ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference

<p><em>Research has been conducted on the antihyperglycemic of yogurt in male white mice. Yogurt contains probiotics that have a positive effect on both type 1 and types 2 diabetes mellitus. Yogurt is given for 3 weeks at a dose of 200 grams / Kg BW / day and 400 grams / Kg BW / day to mice that have been alloxan-induced. As a comparison, a positive control group was created, the alloxan-induced mice group, but not given yogurt and a negative control group, the mice group without any treatment. After 3 weeks of treatment with yogurt, it was found that there was a significant difference between the average blood glucose levels in the mice of the 200 grams/ Kg BW group and the 400 grams / Kg BW dose with the positive control group. From the data on the percentage increase in blood glucose levels of mice, it is known that by giving yogurt for 3 weeks, reducing the high percentage of increase in blood glucose levels in mice. From these data, it can be concluded that the administration of yogurt with a dose of 200 grams / Kg / day and 400 grams / Kg / day can help reduce blood glucose levels in alloxan-induced mice.</em></p><p> </p><p><em>Telah dilakukan penelitian mengenai daya antihiperglikemia yogurt pada mencit putih jantan. Yogurt mengandung probiotik  yang memberikan pengaruh positif terhadap diabetes mellitus baik tipe 1 maupun tipe 2. Yogurt diberikan selama 3 minggu dengan dosis 200/Kg BB/hari dan 400 gram/Kg BB/hari kepada mencit yang telah diinduksi aloksan. Sebagai pembanding dibuat kelompok kontrol positif yaitu kelompok mencit yang diinduksi aloksan, namun tidak diberi yogurt dan kelompok kontrol negatif, yaitu kelompok mencit tanpa perlakuan apapun. Setelah 3 minggu perlakuan dengan yogurt, didapatkan hasil bahwa terdapat perbedaan bermakna antara rata-rata kadar glukosa darah mencit kelompok dosis 200 gram/Kg BB dan dosis 400 gram/Kg BB dengan kelompok kontrol postif. Dari data persentase kenaikan kadar glukosa darah mencit, diketahui bahwa dengan pemberian yogurt selama 3 minggu, mengurangi tingginya persentase kenaikan kadar glukosa darah mencit.dari data-data tersebut, dapat disimpulkan bahwa pemberian yogurt dengan dosis 200 gram/Kg BB/hari dan 400 gram/Kg BB/hari dapat membantu menurunkan kadar glukosa darah mencit yang diinduksi aloksan.</em></p>

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