scholarly journals Therapeutic effect of mango seed extract in diabetes mellitus.

2019 ◽  
Vol 26 (09) ◽  
pp. 1551-1556
Author(s):  
Arfa Azhar ◽  
Kausar Aamir ◽  
Farah Asad ◽  
Hamid Ali Kazi ◽  
Mohammad Umair Farooqui
Keyword(s):  
Diabetes Mellitus ◽  
Therapeutic Effect ◽  
Ethanolic Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Control Group ◽  
Albino Rats ◽  
Common Disease ◽  
Mango Seed

Background: Diabetes mellitus is very common disease; the oral hypoglycemic drugs are utilized for diabetes. In Asia therapeutic plants are extensively used for cure of widespread variation of illnesses. Objectives: To study the Therapeutic effect of mango seed extract on diabetes Mellitus. Study Design: A prospective study and longitude. Setting: This study was conducted at Jinnah Post graduate Center, Karachi. Period: From June 2016 to June 2017. Material and Methods: Mango seeds were taken out with ethanol and used for study. The antidiabetic action of mango seeds extracts were well-thought-out at 100 and 200 mg/kg b.wt. The antidiabetic effect of mango seeds extracts were matched with tolbutamide 500 mg/kg b.wt. Result: The ethanolic extract of mango seeds (200 mg/kg) revealed major (p< 0.01) hypoglycemic outcome in fasted diabetic rats, when matched with normal control group. The mango seed extracts were significantly increased insulin level and decreased HbA1c levels at the dose level (100, 200 mg/kg) in alloxone encouraged diabetic rats. Conclusion: The ethanolic extract of mango seeds ensuring important antidabatic outcome compared to aloxone tempted diabetes in albino rats and its stimulating insulin production in pancreas of albino rats.

scholarly journals The Effects of Grape Seed Extract on Insulin, Adiponectin and Resistin Levels in Diabetic Rats

2021 ◽  
Vol 9 (4) ◽  
pp. 709-713
Author(s):  
İnci Söğütlü ◽  
Nihat Mert ◽  
Handan Mert ◽  
Leyla Mis ◽  
Halil Cumhur Yılmaz ◽  
...  
Keyword(s):  
Insulin Level ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Control Group ◽  
Albino Rats ◽  
Diabetes Group ◽  
Positive Effects ◽  
Wistar Albino Rats

In this study, the effects of grape seed extract on insulin, adiponectin and resistin levels in diabetic rats were aimed to investigate. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats were used. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats randomly divided into four groups of eight each: control group (C), group with diabetes (DM), grape seed extract group (GSE), diabetes mellitus and grape seed extract group (DM+GSE). The diabetic group 45 mg/kg single dose of streptozotocin was administered intraperitoneally, and the grape seed extract groups (DM+GSE and GSE) grape seed extract was given orally every day with an intragastric tube for 20 days (0.6 ml/rat). Blood samples were taken from the hearts of rat’s end of the experiment. The sera obtained were used for insulin, adiponectin and resistin analysis measured via ELISA with commercial kits. The insulin and adiponectin levels of the rats in the diabetes + grape seed extract group were found to be higher than those in the diabetes group. There was no significant change in the resistin level differences between the groups. As a result; It is possible to say that grape seed extract has an increasing effect on insulin level and adiponectin levels of diabetic rats are decreased due to diabetes and it has no significant effect on resistin levels. Detected by the positive effects of grape seed extracts, it may be useful to use for the regulating of the diabetes in human.

scholarly journals Changes in Oxidative Stress and Antioxidant Enzyme Activities in Streptozotocin-Induced Diabetes Mellitus in Rats: Role ofAlhagi maurorumExtracts

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
S. A. Sheweita ◽  
S. Mashaly ◽  
A. A. Newairy ◽  
H. M. Abdou ◽  
S. M. Eweda
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Glucose Levels ◽  
Blood Glucose Levels

Alhagi maurorum(camel thorn plant) is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. No previous study has been conducted before onA. maurorum extractsas an antioxidative stress and/or antidiabetic herb in STZ-induced DM in rats. Therefore, four groups of rats were allocated as control (C), STZ-induced DM (D), and STZ-induced DM supplemented with 300 mg/kg BW of either aqueous extract (WE) or ethanolic extract (EE) ofA. maurorum. The plasma levels of glucose, TG, TC, LDL-C and VLDL-C, MDA, and bilirubin and the activities of transaminases and GR were significantly increased in the diabetic group. Also, diabetic rats showed severe glucose intolerance and histopathological changes in their livers. In addition, levels of insulin, total proteins, GSH, and HDL-C and the activities of SOD, GPx, and GST were significantly decreased in the diabetic rats compared to those of the control group. The ingestion ofA. maurorumextracts lowered the blood glucose levels during the OGTT compared to the diabetic rats and restored all tested parameters to their normal levels with the exception of insulin level that could not be restored. It is concluded thatA. maurorumextracts decreased elevated blood glucose levels and hyperlipidemia and suppressed oxidative stress caused by diabetes mellitus in rats.

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

scholarly journals BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (4) ◽  
pp. 257
Author(s):  
Mutiara Indah Sari ◽  
Maya Anjelir Antika ◽  
Dwi Rita Anggraini
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Wistar Rat ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Control Group Design ◽  
Glucose Levels

 Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

scholarly journals Anti-hyperglycemic Effects of Diacerein in Alloxan-Induced Diabetes Mellitus in Wistar Albino Rats

2020 ◽  
pp. 107-113
Author(s):  
Arsalan Uqaili ◽  
Samia Siddiqui ◽  
Roomi Aijaz ◽  
Yar Muhammad Nizammani ◽  
Navaid Kazi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Group B ◽  
Group D

Objective: To determine the anti-hyperglycemic effects of interleukin-1 inhibitor (diacerein) in alloxan induced diabetic albino wistar rats. This experimental study was performed at the Department of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam within 6 months from April 2016 to September 2016. Total of 160 adult Albino Wistar Rats having an average of 200 to 300 grams body weights were selected. Animals were categorized into 4 groups as; Group A (n=15): Control rats – receive 0.9% normal saline as placebo Experimental Groups Group B (n=15): Experimental Control (Diabetic rats) - Alloxan50 mg/kg body weight intraperitoneal. Group C (n=15): Diabetic rats + Diacerein (30 mg/kg/day) orally daily. Group D (n=15): Diabetic rats + Diacerein (50 mg/kg/day) orally daily. Animals were kept and treated as per the NIH Guideline for Use and Care of Laboratory Animals. Diabetes mellitus was induced via a single intraperitoneal injection of 50 milligram/kg alloxan monohydrated dissolved in aseptic 0.9% saline. After 72 hours, blood specimens were taken from the caudal vein of the rats and glucose level>200 mg/dL was taken as diabetes. Experimental rats were given diacerein approximately 30 and 50 mg orally for 6 weeks. At the completion of experiment the body weight was measured of each animal by electronic measuring balance and blood sample was taken from each animal of all groups to assess the blood glucose level and HbA1c level. Data were recorded via self-made proforma and analysis was done by using SPSS version 20. Results: Average body weight of Diabetic control (Group B) was 193.33±22.50 grams, which was lower in contrast to Diacerein treated group C 202.47±25.70 grams and significantly lower as compared to Diacerein treated group D as  212.6±23.43 grams. A significant increase in blood glucose levels 182.07±10.63 mg/dl was noted in the Diabetic control (Group B) compared to Diacerein treated group C (110.13± 8.54 mg/dl) and group D (85.87±8.41 mg/dl) (P=0.001). HbA1c was markedly raised in the Group B- diabetic controls, while diacerein treated diabetic rats (groups C and D) showed a significant decrease in HbA1c (P=0.001). Conclusion: It was concluded that Diacerein achieves the Euglycemic state by reducing the levels of blood glucose and glycated hemoglobin (HbA1c) in Alloxan-Induced diabetes mellitus in Wistar Albino Rats.

scholarly journals Effect of Ethanolic Extract of Swietenia mahagoni Seeds on Experimentally Induced Diabetes Mellitus in Rats

1970 ◽  
Vol 6 (2) ◽  
pp. 70-73 ◽  
Author(s):  
SMM Mahid-Al-Hasan ◽  
MI Khan ◽  
BU Umar
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Beta Cells ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Control Group ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

Diabetes mellitus is a heterogeneous group of syndromes characterized by an elevation of blood glucose caused by a relative or absolute deficiency of insulin. The incidence of diabetes is growing rapidly worldwide. Drug treatment for diabetes mellitus is expensive and carries risks for many adverse effects. Bangladesh is a rich emporium of medicinal plants useful in the treatment of diabetes. The study was performed to investigate the blood glucose lowering effect of Swietenia mahagoni seeds in experimentally induced diabetic rats. Twenty four healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Diabetes was induced by alloxan (120 mg/kg body weight). Administration of ethanolic extract of Swietenia mahagoni seeds (group-B) produced no significant change in the blood glucose level as compared to control (group-A). Administration of ethanolic extract of Swietenia mahagoni seeds in group-D produced a significant reduction in blood glucose level as compared to diabetic control (group-C). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group-C whereas retaining of islets and few degranulations of beta cells of pancreas found in group-D. These observations and results provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. Key words: Diabetes mellitus; Swietenia mahagoni; Alloxan; Rat; Blood glucose. DOI: http://dx.doi.org/10.3329/fmcj.v6i2.9203 FMCJ 2011; 6(2): 70-73

scholarly journals Antidiabetic Activity of Extracts of Pithecellobium dulce Benth Leaves in Alloxan Induced Diabetic Rats

2016 ◽  
Vol 8 (05) ◽  
Author(s):  
V. S. Mul ◽  
N. Naikwade ◽  
C. Magdum ◽  
V. Jagtap
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Glycogen Level

The objective of the present study was to study effect of Pithecellobium dulce Benth (P. dulce) leaves in alloxan induced diabetic rats. The P. dulce leaves were extracted by maceration and soxhelation method by using water and ethanol as solvent. Acute toxicity study was performed according to OECD 425 guidelines for both aqueous and ethanolic extracts of P. dulce leaves. The dose of 200 mg/kg and 400 mg/kg was selected for further studies. Animals were rendered diabetic by administration of alloxan (130 mg/kg, i.p.). The albino rats were divided in to seven groups with five animals in each group. Diabetic animals were treated with aqueous and ethanolic extract for 20 days. Then blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, aspartate aminotransferase (AST), alanine transaminase (ALT) and glycogen level in liver, muscle and kidney were estimated according to standard procedures. The result shows significant decrease in blood glucose, triglyceride, total cholesterol, urea, uric acid, creatinine, AST and ALT level when compared to diabetic group. The liver and muscle glycogen level was increased significantly in extract treated groups when compared to diabetic control group. Both extract of P. dulce posses antidiabetic and hypolipidemic potential.

scholarly journals Effect of turmeric on alloxan induced diabetes mellitus in albino rats

2019 ◽  
Vol 8 (2) ◽  
pp. 264
Author(s):  
Mugdha Rajeeva Padhye ◽  
Sangita Devrao Jogdand
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Adverse Effects ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Organ Systems ◽  
Mean Blood Glucose

Background: Diabetes mellitus is a chronic metabolic disorder of glucose metabolism characterised by hyperglycaemia. Long standing diabetes mellitus leads to various complications affecting multiple organ systems. Management of diabetes mellitus includes lifestyle modification and pharmacotherapy. Pharmacotherapy of diabetes mellitus includes a wide variety of drugs that help in achieving adequate glycaemic control. Anti-diabetic medications are however associated with several adverse effects. Phytochemicals are being used extensively for the treatment of various diseases. Use of phytochemicals would minimize adverse effects due to various anti-diabetic drugs and improve patient compliance. In the present study, authors studied the effect of turmeric on alloxan induced diabetes mellitus in albino rats.Methods: Albino Wistar rats of either sex weighing 180 - 250grams were utilized for the present study. Diabetes mellitus was induced by intraperitoneal administration of alloxan. Ethanolic extract of turmeric was administered to diabetic rats daily orally for duration of 28 days. Blood glucose levels were monitored using glucometer before and after intervention with turmeric.Results: Statistically significant reduction in mean blood glucose levels (p value <0.05) was seen after intervention with turmeric in diabetic rats. There was a significant reduction in mean blood glucose levels.Conclusions: Ethanolic extract of turmeric showed antihyperglycemic effect in diabetic rats.

scholarly journals CLERODENDRUM VOLUBILE ETHANOL LEAF EXTRACT: AN EFFECTIVE MITIGATOR AGAINST DOXORUBICIN-INDUCED HEPATORENAL TOXICITIES IN RATS

2021 ◽  
Vol 2 (1) ◽  
pp. 01-09
Author(s):  
O. B. Akinsanya ◽  
P. F. Ayodele ◽  
O. F. Onifade ◽  
M. O. Salimom
Keyword(s):  
Diabetes Mellitus ◽  
Beta Cells ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Diabetic Rats ◽  
Persea Americana ◽  
Control Group ◽  
Albino Rats ◽  
Pancreatic Islet Cells ◽  
Standard Drug

Diabetes mellitus develops either due to insufficient insulin secretion or lack of insulin resulting from damaged pancreas beta cells. Dyslipidaemia is an acute complication in diabetes mellitus. The aim of this study was to investigate the extenuating effects of T. cattapa leaves and P. americana seed aqueous extracts on streptozotocin-induced lipids profile perturbation and pancreatic damage in experimental rats. Thirty male albino rats were randomly divided into six groups (n = 5). Group 1: (negative control), group 2: 80 mg/kgbwt streptozotocin (positive control), group 3: (80 mg/kgbwt streptozotocin + 200 mg/kgbwt T. catappa leave extract), group 4: (80 mg/kgbwt streptozotocin + 200 mg/kgbwt Persea americana seed extract), group 5: (80 mg/kgbwt streptozotocin + 200 mg/kgbwt extracts-mixture), group 6: (80 mg/kgbwt streptozotocin + 5 mg/kgbwt glibenclamide (standard drug). A single dose of streptozotocin was administered to the rats intraperitoneally; the extracts and glibenclamide were administered orally for 21 days, after which their pancreas was excised for histology. Thereafter, extracts of T. catappa leaves and P. americana seed on serum lipid levels imbalance in streptozotocin-induced diabetic rats were investigated. Histological studies showed a degenerative effect of the pancreatic islet cells of strotozotocin-induced groups. Results showed improvement in the regulation of lipid metabolism as well as the regeneration of the beta cells of pancreas on treatment with T. catappa leaves and P. americana seed. The extracts both equally exert significant antidyslipidemic effects in diabetic rats, in view of the extenuating effects of the extracts on pancreatic islet cells.

scholarly journals Hyperglycaemic Environment: Contribution to the Anaemia Associated with Diabetes Mellitus in Rats Experimentally Induced with Alloxan

Anemia ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Oseni Bashiru Shola ◽  
Fakoya Olatunde Olugbenga
Keyword(s):  
Diabetes Mellitus ◽  
Reticulocyte Count ◽  
Test Group ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Plasma Haemoglobin ◽  
Sterile Normal Saline ◽  
Red Blood Cell Count ◽  
Glucose Plasma

Background. Diabetes mellitus characterized by hyperglycaemia presents with various complications amongst which anaemia is common particularly in those with overt nephropathy or renal impairment. The present study has examined the contribution of the hyperglycaemic environment in diabetic rats to the anaemia associated with diabetes mellitus. Method. Sixty male albino rats weighing 175–250 g were selected for this study and divided equally into control and test groups. Hyperglycaemia was induced with 170 kgbwt−1alloxan intraperitoneally in the test group while control group received sterile normal saline. Blood samples obtained from the control and test rats were assayed for packed cell volume (PCV), haemoglobin (Hb), red blood cell count (RBC), reticulocyte count, glucose, plasma haemoglobin, potassium, and bilirubin.Result. Significant reduction (P<0.01) in PCV (24.40±3.87versus40.45±3.93) and haemoglobin (7.81±1.45versus13.39±0.40) with significant increase (P<0.01) in reticulocyte count (12.4±1.87versus3.69±0.47), plasma haemoglobin (67.50±10.85versus34.20±3.83), and potassium (7.04±0.75versus4.52±0.63) was obtained in the test while plasma bilirubin showed nonsignificant increase (0.41±0.04versus0.24±0.06).Conclusion. The increased plasma haemoglobin and potassium levels indicate an intravascular haemolytic event while the nonsignificant increased bilirubin showed extravascular haemolysis. These play contributory roles in the anaemia associated with diabetes mellitus.

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