scholarly journals Utilization of Andong Leaf Extract (Cordyline fruticosa) as an Alternative for Coloring the Eggs of Intestinal Nematode

2021 ◽  
Vol 2 (2) ◽  
pp. 108-112
Author(s):  
Supriyanto ◽  
Linda Triana

Microscopic examination is essential to find the type of worm that causes infection. Direct examination of worm eggs needs to use staining to distinguish between worm eggs and food residues that are not completely digested. The dyes commonly used are synthetic. The purpose of this study was to replace synthetic dyes with natural dyes with Andong (Cordyline Fruticosa) leaf juice. It can be used as an alternative or a substitute for dyes. This research method is descriptive, conducted at the Parasitology Laboratory of Poltekkes Kemenkes Pontianak. The population in this study was the juice of the leaves of Andong (Cordyline Fruticosa). The method of inspection is direct preparation with an object glass as is routinely done, but the dye is replaced with Andong leaf juice (Cordyline Fruticosa). The results showed that natural dye Andong leaf juice (Cordyline Fruticosa), gave a clear color to the absorption of intestinal nematode worm eggs as the test target. This study concludes that the juice of the leaves of Andong (Cordyline Fruticosa) with alcohol solvent can be used as an alternative dye for the examination of intestinal nematode worm eggs.

Pro Food ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 643
Author(s):  
Dhanang Puspita ◽  
Jacob Lukas Alexander Uktolseja

ABSTRACT Color is very important in the food industry. The need for food coloring requires manufacturers to use synthetic dyes that have the potential to cause poisoning and cancer. One source of natural dyes comes from bacteria that are symbiotic with coral reefs. The purpose of this study is to isolate and characterize the bacterial pigment that has symbiosis with Montipora sp. The research method consisted of bacterial isolation and identification, pigment identification with UV-Vis spectrofotometer (200 – 800 nm) and TLC. The results of isilation and identification showed that Rhodococcus sp is dominant bacterial which is produces of carotenoiids for self defense from UV rays. The pigment found in Rhodococcus sp has the potential as a natural pigment for food coloring. Keywords: carotenoids, Montipora, pigment, Rhodococcus sp. ABSTRAK Warna sangat penting dalam industri pangan. Kebutuhan pewarna makanan menuntut produsen memakai bahan pewarna sintetik yang berpotensi menyebabkan keracunan dan kanker. Salah satu sumber pewarna alami berasal dari bakteri yang bersimbiosis dengan terumbu karang. Tujuan dari penelitian ini adalah mengisolasi dan mengkarakterisasi pigmen bakteri yang bersimbion Montipora sp. Metode penelitian terdiri dari isolasi dan identifkasi bakteri, identifikasi pigmen dengan spektrofotometer UV-Vis (200 – 800 nm) dan KLT. Hasil isolasi dan identifikasi bakteri berjenis Rhodococcus sp dan piigmen yang dihasilkan adalah karotenoid yang digunakan sebagai pertahanan diri dari sinar UV. Pigmen yang terdapat pada Rhodococcus sp berpotensi sebagai pigmen alami untuk pewarna pangan. Kata kunci: karotenoid, Montipora, pigmen, Rhodococcus sp.


Author(s):  
Imam Safir Alwan Nurza

Hanjuang (Cordyline fruticosa) is perennial plant which has function as protective plant and barrier in rice fields or fields. The research purpose was to determine Hanjuang (Cordyline fruticosa) plant based on morphology and anatomy. The research method used was descriptive qualitative. Samples were taken at Bogor Botanical Gardens. The anatomy Observed under microscope light with magnification 100-400 times. The research resulted that leaves morphology showed pointed apical and base, arrangement pinnate bones, flesh like paper, lanceolate shape, smooth surface, choppy edges, bright purple when exposed to sunlight and purple when is not, and type leaf sheath. Moreover, leaves, stems, and roots have support tissue in form of colenchyma. The anatomy of stem and root showed transport tissue but xylem observed larger than its phloem. Root showed radially shape. Leaves has an actinositic stomata and content ergastic in the form of needle crystals. Leaves could be seen purple in water and changes when to yellow and green after some drops of basic solutions. It showed that Hanjuang (Cordyline fruticosa) leaves contain anthocyanin but no carotenoid.


2017 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
Anita Oktari ◽  
Ahmad Mu’tamir

Worm infections or can be called by worming included into an infection caused by a parasite. Soil Transmitted Helminths Intestinal Nematodes are worms groups are in their life cycle to reach the infective stage requires soil with certain conditions. Eosin 2% is the dye that is used in the examination of Intestinal Nematode worm eggs. Red fruit (Pandanus sp.) which is a natural plant material and acidic contain carotenoids which produces orange-red pigment. Beta carotene is the predominant pigment of red-orange color that is found naturally in plants and fruits. The aim of this research is to determine the best concentration from variation of red fruit (Pandanus sp.) juice that optimally to color the eggs of the worm. Research conducted experiments with various concentration ratio of red fruit (Pandanus sp.) juice and distilled water (1, 1:1, 1:2, 1:3, 1:4, 1:5). From this research it found that the results indicate the ratio of concentrations of red fruit (Pandanus sp.) juice and distilled water (1:2) can be used as an alternative reagent eosin 2% for their examination of worm eggs. But in the visual field that uses red fruit (Pandanus sp.) juice and distilled water (1:2) still looks much dirt as a nuisance and does not give a contrasting background. It can conclude that red fruit (Pandanus sp.) juice can use to color the eggs of the worm.


2020 ◽  
Vol 4 (1) ◽  
pp. 180-186
Author(s):  
Erni Rouza ◽  
Jufri ◽  
Luth Fimawahib

The purpose of pattern recognition is do the process of classifying an object into one particular class based on the pattern it has, so it can be used to recognize patterns of intestinal nematode worm types. One of the methods used in pattern recognition is by utilizing the artificial neural network method, the artificial neural network is able to represent a complex Input-Output relationship. For that the algorithm used is the perceptron algorithm. Perceptron is one method of Artificial Neural Networks. In the introduction of types of intestinal nematode worms, a computer must be trained in advance using training data and test data, this study discusses how a computer can recognize a pattern of types of intestinal nematode worms using the perceptron method. Based on the results of testing trials with input in the form of worm image scan results, based on the results of the perceptron method testing is able to recognize the pattern recognition of the types of intestinal nematode worms and be able to analyze with the right results of 100% for pinworms patterns, hookworm patterns, and 40- 50% for roundworms, by comparing the output value and the target value entered first.


2020 ◽  
Vol 1 (2) ◽  
pp. 67-71
Author(s):  
Subhan ◽  
Febrina Arfi ◽  
Aminul Ummah

The synthetic dyes Rhodamin B and Methanyl Yellow are still often added to snacks to make the colors of snacks more obvious and people are interested in consuming them. Its use in long-term food products can cause cancer. The purpose of this study was to analyze synthetic dyes in chips, red macaroni, steamed cakes, yellow macaroni, bread jam and guava juice around the Ketapang area of ​​Banda Aceh City. The research method used is the Spot Test Analysis conducted qualitatively at the LPPOM MPU Laboratory in Banda Aceh City. The test results on the three samples namely chips, red macaroni, and steamed cake did not produce a color change reaction that showed Rhodamine B. The test results on yellow macaroni, bread jam and guava juice showed that the three samples tested did not produce a color change reaction indicating the presence of Methanyl Yellow. The conclusion from this test is that snacks have not been identified as Rhodamin B and Methanyl yellow or are free from the contents of the two synthetic dyes.


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Fitriani Kahar ◽  
Quirinus Efendi ◽  
Ichsan Hadipranoto

2019 ◽  
Vol 6 (2) ◽  
pp. 70-77
Author(s):  
Askrening - Askrening

Background: Humans are intestinal nematode hosts whose transmission occurs through soil or soil transmited helminthes, as a place of life and development of worm eggs and larvae before they are transmitted to the human body, especially in children who are used to playing or direct contact with the soil, allowing the nails of Nematode eggs to be exposed the intestine.Purpose: The aim of this study was to determine the presence of intestinal nematode worm eggs on Ranomeeto 11 elementary school students. Methode: The type of research used is descriptive research, which was conducted on 28 June - 2 July 2016. The sample in this study was taken by Simple Random Sampling. The data taken in the form of primary and secondary data with the research instrument is a checklist and examination results. Data analysis techniques and data presentation are descriptive and presented in the form of frequency distribution tables and narrated.Result: The results of research conducted on 37 nail samples of Ranomeeto 11 Elementary School students showed that there were 5 positive samples containing intestinal Nematode eggs which were 4 positive Ascaris lumbricoides (10.82%) and 1 positive Trichuris trichiura (2.70%) and 32 (86 , 48%) negative samples do not contain intestinal nematodes.Conclusion: The conclusion of this study is that there are 5 positive samples of intestinal Nematode worm eggs 13.51% and 32 negative samples 86.48% do not contain intestinal nematode worm eggs.


2020 ◽  
Vol 15 (2) ◽  
pp. 231-241
Author(s):  
Hartiningsih

Naturally dyed Sasirangan is one of the cultural heritage of the Banjar Empire. Originally, the Sasirangan was made using natural dyes. Nowadays, the Sasirangan made by synthetic dyes. The synthetic dyes can produce more various and brighter colors, which is more interesting for the consumer. The synthetic dyes have been reduced and lowered the interest of natural dyed Sasirangan fabric. Along with the less interest in natural dyed Sasirangan fabric, the craftsmen reduction, and other various impacts, it is concerned that the natural dyed Sasirangan fabric which is a heritage will be extinct. Therefore, this research was conducted to give an overview of the existence of the natural dyed Sasirangan fabric and the roles of the government, the craftsmen, as well as the society in their effort to preserve it. The research method is qualitative descriptive. The result shows that the existence of the natural dyed Sasirangan fabric has been significantly increased both for its quantity, which is the rise of the craftsmen’s number, as well as its quality such as the motives diversity and the coloring quality which does not wear off easily. The effort of the government, the craftsmen, and the society in preserving Sasirangan fabric is quite diverse, from the coaching, the training for the general public, the housewives, also the students in the school environment. Even, for maintaining the Sasirangan fabric preservation, the Banjarmasin City government issued a policy in the form of a circular which obliges all the state civil apparatus in the environment of Banjarmasin City government to wear clothing made of natural dyed Sasirangan fabric on a certain day of each month. Keywords: Sasirangan Fabric, Natural Dyes, Preservation, Banjar Culture  ABSTRAK Kain sasirangan pewarna alam  merupakan salah satu warisan budaya Kerajaan Banjar. Kain sasirangan semula  dibuat dengan menggunakan pewarna alam. Seiring dengan perkembangan zaman kain sasirangan kemudian dibuat dengan menggunakan  zat pewarna sintetis. Pewarna sintetis dapat menghasilkan warna yang lebih beragam dan cerah sehingga lebih banyak diminati konsumen,  sehingga minat terhadap sasirangan pewarn alam semakin berkurang. Hal ini pada akhirnya mengakibatkan terus berkurangnya pengrajin sasirangan pewarna alam dan dikhawatirkan kain sasirangan pewarna alam yang merupakan warisan leluhur akan punah. Penelitian ini dilakukan untuk memberikan gambaran keberadaan kain sasirangan pewarna alam dan upaya pelestariannya oleh pemerintah, pengrajin dan masyarakat.  Metode penelitian adalah deskriptif kualitatif. Hasil penelitian menunjukkan produksi kain sasirangan pewarna alam mengalami peningkatan yang cukup signifikan. Kualitasnya juga meningkat dalam hal variasi motif dan warna yang tidak mudah luntur. Upaya pelestarian dari pemerintah, pengrajin  dan elemen masyarakat meliputi pembinaan dan pelatihan terhadap masyarakat umum, ibu-ibu rumah tangga, sampai pada anak didik di lingkungan sekolah. Pemerintah Kota Banjarmasin juga mengeluarkan kebijakan berupa Surat Edaran yang mewajibkan  seluruh ASN di lingkungan Pemerintah Kota Banjarmasin  menggunakan pakaian berbahan kain sasirangan pewarna alam  pada hari tertentu dalam setiap bulannya. Kata Kunci: Kain  Sasirangan,  Pewarna Alam, Pelestarian, Budaya Banjar.  


Lontara ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 42-48
Author(s):  
Anita Anita Anita ◽  
Tuty Widyanti ◽  
Effendy Rasiyanto ◽  
Budiawan S.HI. Karim

        ABSTRACT   Communities on Lae-Lae Island, Makassar City have sanitation facilities that are still poor and very limited with quality far from health standards. This causes people who live in these islands to face various health problems, one of which is the risk of being infected with eggs of intestinal nematode worms Soil-Transmitted Helminths (STH). This study aims to identify the eggs of the intestinal nematode worm Soil Transmitted Helminthes in the feces of people on Lae-Lae Island, Makassar City. This type of research is a laboratory observation with a purposive sampling technique of 10 stool samples. Based on the results of research that has been carried out on 10 faecal samples, it was found 1 positive stool sample for Trichuris trichura worm eggs with distinctive egg-shaped characteristics such as the shape of crock worm eggs or wine barrels and at both ends there are two mucoid plugs. The egg wall is brown from the color of the bile at both ends, it is clear, while the other 9 stool samples are negative the type of worm Trichuris trichura and 9 other samples were negative.    


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