scholarly journals PENGARUH EKSTRAK DAUN BELUNTAS (Pluchea indica (L.) Less) 25% TERHADAP BIOFILM STREPTOCOCCUS MUTANS - in vitro

2018 ◽  
Vol 5 (1) ◽  
pp. 28 ◽  
Author(s):  
Clarissa Bonita Syaravina ◽  
Rizki Amalina ◽  
Eko Hadianto

Background: Bioflm begins with formation of pelicle and within a minutes the colonization of bacteria attached to surface of the teeth. One of early bacteria attached was Streptococcus mutans. This study used 25% beluntas leaf extract in influencing the growth of Streptococcus mutans bioflm. The purpose of study was to investigated effect of 25% beluntas leaf extract on Streptococcus mutans bioflm.Methods: This research was experimental laboratory with post test only control design, consist of four treatment groups, 25% beluntas leaf extract and 0.12% chlorhexidine incubated 24 hours and 48 hours. Bioflm formation was measured by calculating Optical Density using a spectrophotometer. Data analysis was performed using One Way Anova test followed by Post Hoc LSD test.Result: The results showed that beluntas leaf extract could influence theformation of S.mutans bioflm but the effect in inhibiting bioflm formation is still not as good as chlorhexidine. It is known from the results of One Way Anova 25% beluntas leaf extract and 0.12% chlorhexidine incubated for 24 hours and 48 hours showed signifcant difference (p <0.05).Conclusion: The conclusion of this research is the effect of beluntas leaf extract (Pluchea indica (L.) Less) 25% to Streptococcus mutans bioflm in vitro.

e-GIGI ◽  
2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Juvensius R. Andries ◽  
Paulina N. Gunawan ◽  
Aurelia Supit

Abstrak: Minyak cengkeh berguna sebagai antibakteri alami. Minyak esensial dari cengkeh mempunyai fungsi anestetik dan antimikrobial. Zat yang terkandung dalam cengkeh yang bernama eugenol dapat membunuh bakteri termasuk bakteri yang resisten terhadap antibiotika, salah satunya adalah bakteri Streptococcus mutans. Bakteri ini merupakan mikroorganisme penyebab utama terjadinya karies. Tujuan dari penelitian ini yaitu untuk mengetahui efek antibakteri ekstrak cengkeh terhadap bakteri Streptococcus mutans secara in vitro. Penelitian ini merupakan penelitian eksperimental menggunakan post test only control group design. Penelitian ini menggunakan bahan coba ekstrak cengkeh dengan konsentrasi 40%, 60%, dan 80%, Ciprofloxacin, aquades dengan pengulangan sebanyak lima kali. Data dikumpulkan dan dianalisis dengan one-way ANOVA dan post-hoc uji LSD ( = 0,05). Berdasarkan hasil uji statistik penelitian uji efek antibakteri ekstrak cengkeh terhadap bakteri streptococcus mutans secara in vitro, dapat disimpulkaan bahwa ekstrak cengkeh memiliki efek antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans secara in vitro. Hasil uji lanjut post-hoc uji LSD menunjukan daya hambat ekstrak cengkeh 40%, 60%, 80%, lebih kecil (p<0,05) dalam menghambat Streptococcus mutans secara in vitro dibandingkan Ciprofloxacin. Kata Kunci: Ekstrak cengkeh, Streptococcus mutans.   Abstract: Clove oil is useful as a natural antibacterial agent, essential oil of clove has anesthetic and antimicrobial effect. Substances contained in clove called eugenol can kill bacteria including antibiotic resistant bacteria, one of which is the bacteria Streptococcus mutans. This bacteria is a major cause for caries. The purpose of this study was to mengetahui clove extrack antibacterial effects againts Streptococcus mutans bacteria in vitro. This study is an experimental study using a post test only control group design. This research try using clove extract with a concentration of 40%, 60%, and 80%, Ciprofloxacin, aquades repetition five times. Data collected and analyzed by one-way ANOVA and post-hoc LSD test (α = 0.05). Based on the results of the statistical test to test the effects of anti-bacterial research clove extracts against Streptococcus mutans bacteria in vitro, can disimpulkaan that clove extracts have antibacterial effects in inhibiting the growth of Streptococcus mutans bacteria in vitro.further test result post-hoc LSD test shoved its inhibitory clove extract 40%, 60%, 80% smaller (p<0,05)in hibiting Streptococcus mutans in vitro compared Ciprofloxacin. Keywords: clove extract, Streptococcus mutans


e-GIGI ◽  
2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Yaromis Wenda ◽  
Pemsi M. Wowor ◽  
Michael A. Leman

Abstract: Currently, oral health requires comprehensive handling by dentists and other health professionals. Osteomyelitis is an infection that occurs in bone tissue and bone marrow of the jaw and/or cortical bones, predominantly caused by Staphylococcus aureus. Medicinal plants in Indonesia have been widely used as traditional medicine inter alia stevia (Stevia rebaudiana Bertoni M.). This stevia plant has components that are typical natural sweetener and stevioside which has the characteristics as antibacterial, antiviral, anti-inflammatory, antimicrobial, and antifungal, and other active substances including tannins, alkaloids, flavonoids, and phenol. This was an experimental laboratory study with the post test only group design. Subjects were Staphylococcus aureus bacteria. The results showed that stevia leaf extract had inhibition zone to Staphylococcus aureus growth of 10.32 mm which was categorized as strong according to Davis and Stout 1971. Conclusion: Stevia leaf extract (Stevia rebaudiana Berrtoni M.) had strong inhibition to Staphylococcus aureus.Keywords: osteomyelitis, stevia (Stevia rebaudiana Bertoni M.), Staphylococcus aureusAbstrak: Dewasa ini kesehatan gigi dan mulut memerlukan penanganan secara komperhensif oleh dokter gigi maupun tenaga kesehatan lainnya. Salah satu penyakit yang sering dijumpai yaitu osteomielitis pada jaringan tulang dan sum-sum tulang rahang dan/atau korteks tulang dengan penyebab utama ialah bakteri Staphylococcus aureus. Tanaman herbal di Indonesia telah banyak digunakan sebagai obat tradisional; salah satunya ialah tanaman stevia (Stevia rebaudiana Bertoni M.). Tanaman stevia memiliki komponen yang bersifat pemanis alami, stevioside yang berefek antibakteri, antivirus, antiinflamasi, antifungsi, dan antimikroba, serta zat aktif di antaranya ialah tannin, alkaloid, flavonoid, dan fenol. Jenis penelitian ini ialah eksperimental laboratorik dengan post test only group design. Subyek penelitian ialah bakteri Staphylococcus aureus. Hasil penelitian memperlihatkan bahwa ekstrak daun stevia (Stevia rebaudiana Bertoni M.) mempunyai rerata zona hambat terhadap pertumbuhan bakteri Staphylococcus aureus sebesar 10,32 mm yang tergolong kuat menurut Davis dan Stout 1971. Simpulan: Ekstrak daun stevia (Stevia rebaudiana Berrtoni M.) memiliki daya hambat yang kuat terhadap bakteri Staphylococcus aureus.Kata kunci: osteomielitis, stevia (Stevia rebaudiana Bertoni M.), Staphylococcus aureus


2016 ◽  
Vol 2 (2) ◽  
pp. 60
Author(s):  
Devi Puspita Handayani ◽  
Dewi Puspitasari ◽  
Nurdiana Dewi

The effect of immersion of decoction water of Red Betel Leaf (Piper crocatum) on the surface hardness of composite resin. The purpose of this study was to determine the effect of immersion of decoction water of red betel leaf on the surface hardness of composite resin compared to alcoholic mouth rinse. It was a pure experimental study with post-test only with control group design. The total samples were 27 samples divided into 2 treatment groups and 1 control group, each of which consisted of 9 samples. The treatment groups were immersed in the decoction water of red betel leaf and alcoholic mouth rinse. The control group was immersed in aquadest. After the immersion, the samples were measured using Vickers Micro hardness Tester. Analysis with one way anova and post hoc Bonferroni showed a significant difference (p<0.05) on the surface hardness of composite resin after being immersed in decoction water of red betel leaf(79,81±3,76) kg/mm2 and alcoholic mouth rinse (67,11±2,51) kg/mm2. Based on this research, it can be concluded that there was an effect of immersion of decoction water of red betel leaf if compared with alcoholic mouth rinse. The value of surface hardness of composite resin immersed in alcoholic mouth rinse was lower than the decoction water of red betel leaf.ABSTRAKPenelitian ini bertujuan untuk mengetahui efek perendaman dalam rebusan daun sirih merah terhadap kekerasan permukaan resin komposit bila dibandingkan dengan obat kumur beralkohol. Penelitian ini merupakan penelitian eksperimental murni dengan post-test only with control group design. Jumlah sampel sebanyak 27 yang dibagi dalam 2 kelompok perlakuan dan 1 kelompok kontrol dengan masing-masing kelompok sebanyak 9 sampel. Kelompok perlakuan direndam dengan air rebusan daun sirih merah dan obat kumur beralkohol. Kelompok kontrol direndam dengan akuades steril. Setelah itu dilakukan pengukuran menggunakan Vickers Microhardness Tester. Hasil uji One Way Anova dan Post Hoc Bonferroni menunjukkan bahwa terdapat perbedaan bermakna nilai kekerasan permukaan resin komposit yang direndam dengan air rebusan daun sirih merah (79,81±3,76) kg/mm2 dan obat kumur beralkohol (67,11±2,51) kg/mm2 dengan nilai kemaknaan (p<0,05). Berdasarkan penelitian yang dilakukan dapat diambil kesimpulan bahwa terdapat efek lebih rendah pada perendaman dalam rebusan daun sirih merah terhadap kekerasan permukaan resin komposit bila dibandingkan dengan obat kumur beralkohol. Nilai kekerasan permukaan resin komposit yang direndam obat kumur beralkohol lebih rendah dibandingkan air rebusan daun sirih merah.


2018 ◽  
Vol 14 (2) ◽  
pp. 84-91
Author(s):  
Andri Tria Raharja ◽  
Nanda Alfian Mahardhika ◽  
Jeane Betty Kurnia Jusuf ◽  
Julianur Julianur ◽  
Januar Abdilah Santoso

Penelitian ini bertujuan untuk tingkat efektivitas penggunaan macromedia flash 8 terhadap hasil belajar materi penyakit menular seksual di SMA Negeri 1 Loa Kulu. Penelitian ini menggunakan metode pendekatan kuantitatif. Jenis penelitian yang digunakan pada penelitian ini adalah posttest-only control design. Analisis data yang digunakan dalam penelitian ini adalah One way anova. Subyek uji coba diambil menggunakan purposive sampling. Tempat penelitian dibatasi pada Sekolah Menengah Atas yang berada di SMAN 1 Loa Kulu dengan jumlah 60 siswa. Hasil analisis data yang diperoleh pada data uji normalitas yang didapat adalah nilai Sig. Kolmogorov-Smirnov Post Test A atau kelompok dengan menggunakan treatment = 0,087 dan Sig. Kolmogorov-Smirnov Post Test B atau kelompok tidak menggunakan treatment = 0,068 lebih besar dari taraf uji signifikan 0,05. Maka dapat disimpulkan bahwa nilai berdistribusi normal. Sedangkan pada uji homogenitas nilai Sig. Post Test A dan Post Test B diketahui sebesar 0,263. Maka dapat disimpulkan data homogenitas = 0,263 > 0,05 dan nilai post test A dan post test B memiliki varian yang sama dan homogen. Setelah melakukan uji normalitas dan homogenitas, langkah selanjutnya adalah uji-F dengan one way anova. Data yang diperoleh Fhitung > Ftabel yaitu 12,64 > 4,01. Maka dapat disimpulkan bahwa data ada perbedaan yang signifikan. Effectiveness of the use of macromedia flash 8 on learning outcomes of students of sexually transmitted diseases in Senior high school AbstractThis study aims to determine the effectiveness of the use of macromedia flash 8 on the results of learning sexually transmitted diseases material at State Senior High School 1 Loa Kulu. This study uses a quantitative approach. The type of research used in this study was posttest-only control design. The data analysis used in this study is One way ANOVA. The subjects of the trial were taken using purposive sampling. The research location is limited to senior high schools located at SMAN 1 Loa Kulu with a total of 60 students. The results of data analysis obtained on the normality test data obtained are the Sig. Kolmogorov-Smirnov Post Test A or group using treatment = 0.087 and Sig. Kolmogorov-Smirnov Post Test B or group not using treatment = 0.068 greater than the significant test level of 0.05. Then it can be concluded that the value is normally distributed. While the test homogeneity of the Sig. Post Test A and Post Test B are known to be 0.263. Then it can be concluded that homogeneity data = 0.263> 0.05 and the post test A and post test B values have the same and homogeneous variants. After conducting the normality and homogeneity test, the next step is the F-way test with one way ANOVA. Data obtained by Fcount> Ftable is 12.64> 4.01. Then it can be concluded that the data have significant differences.


2020 ◽  
Vol 11 (1) ◽  
pp. 65
Author(s):  
Charles Manurung ◽  
Melysa Loeslim ◽  
Setia Budi Tarigan

This research was conducted to determine the effectiveness test of papaya leaf extract with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 90% and 100% against staphylococcus epidermidis bacteria. Staphylococcus epidermidis is a gram-positive bacterium in the form of cocci and is harmful to humans. This research was conducted with an experimental method with a post-test only design and sampling with a purposive sampling method. The investigation of the effectiveness of papaya leaf extract against staphylococcus epidermidis was done by diffusion using paper discs, namely by calculating the diameter of the bacterial inhibition zone of paper discs that have been moistened with papaya leaf extract. The results of this study were analyzed out with the One-Way ANOVA test followed by the Post Hoc Test. The test showed that there were significant differences from each treatment given with a 95% confidence index. The bacteria treated with papaya leaf extract 100% against staphylococcus epidermidis had the highest average inhibitory zone power compared to other concentrations of 14.97 mm. The papaya leaf extract 100% has the best antibacterial effect compared to other concentrations because the best inhibitory zone power is found on those extract concentrations.


2021 ◽  
Vol 1 (01) ◽  
pp. 9-18
Author(s):  
Nurasisa Lestari ◽  
Masriadi Masriadi ◽  
Maqhfirah Amiruddin ◽  
Sarahfin Aslan ◽  
Yustisia Puspitasari ◽  
...  

Pendahuluan : Streptococcus mutans adalah salah satu bakteri yang banyak ditemukan pada rongga mulut, dimana bakteri Streptococcus mutans dapat menghambat proses penyembuhan dry socket yang dipelajari oleh Rozantis, untuk itu pencegahan infeksi dapat dilakukan dengan memberikan antibiotik. Cabai rawit (Capsicum frutescens L) adalah salah satu tanaman herbal yang memiliki efek sebagai anti mikroba terhadap bakteri. Tujuan penelitian ini adalah untuk mengetahui ekstrak cabai rawit (Capsicum frutescens L) dalam menghambat pertumbuhan bakteri Streptococcus mutans. Tujuan Penelitian : Untuk mengetahui efektivitas daya hambat ekstrak cabai rawit (capsicum frutescens l) dalam menghambat pertumbuhan bakteri streptococcus mutans secara in vitro. Bahan dan Metode: Penelitian ini menggunakan metode Eksperimental Laboratorium yaitu pengujian yang dilakukan di laboratorium dengan bentuk penelitian berupa Post Test Only Control Design dan pengambilan sampel dengan Purposive Sampling menggunakan 4 perlakuan dan 6 kali pengulangan. Sampel penelitian yang digunakan adalah koloni bakteri Streptococcus mutans. Pengenceran ekstrak cabai rawit (Capsicum frutescens L) yaitu menggunakan 3 konsentrasi (25%, 50%, dan 100%). Hasil : Hasil penlitian ini menunjukkan diameter zona daya hambat bakteri Streptococcus mutans pada ekstrak cabai rawit (Capsicum frutescens L) konsentrasi 25% sebesar 10,09±0,83mm, konsentrasi 50% sebesar 12,32 ± 0,89mm dan konsentrasi 100% sebesar 16,00 ± 0,86mm dan berdasarkan uji statistic memperoleh nilai signifikan P<0.01. Kesimpulan : Hipotesis alternatif penelitian ini diterima dan hasil penelitian ini menunjukkan bahwa terdapat nya efektivitas ekstrak cabai rawit (Capsicum frutescens L) konsentrasi 25%, 50% dan konsentrasi 100% dalam mengahmbat bakteri Streptococcus mutans.


2018 ◽  
Vol 6 (2) ◽  
pp. 131-138
Author(s):  
Ariani Ariani ◽  
Gemala Anjani ◽  
Muchlis Achsan Udji Sofro ◽  
Kis Djamiatun

Background : Sago worm flour (Rhyinchophorus ferrugineus)is a coconut beetle larvae that is processed into flour.This flour contains antioxidants as well as arginine, both of which play a role in modulating oxidative stress including NO involved in immunopathology of cerebral malaria.Objectives : To prove the immunomodulator role of sago worm flour in decreasing circulation  NO level at mice  who received standard antimalarial therapy  Dihydroartemisinin Piperaquine (DHP)Methods : This study was randomized control group post test only design by using 23 Swiss mice which divided into 5 group consist of : K(-) normal mice; K(+) inoculated woth Plasmodium berghei ANKA (PbA); X1 received DHP; X2 received sago worm flour; X3 received both of sago worm flour and DHP. All treatment groups X1,X2 and X3 were inoculated by PbA before treatmen were given. Serum circulation NO level was assessed by ELISA. statistical analysis used was One Way ANOVA followed by Bonferroni post hoc test.Results : The mean  NO level in K(-), K(+), X1, X2, and X3 were 1.008 μmol/mL, K(+) 1.338 μmol/mL; while at treatment X1 1.143 μmol/mL, X2 1.410 μmol/mL, dan X3 0.886 μmol/mL. One way ANOVA showed that they were significantly different (p=0.001). Bonferroni post hoc test of X2 was  proportional to K(+) (p=1.000), whole had NO level lower than either K(+)(p=0.009) or X2 (p=0.002). Conclusion : The immunomodulatory effects of sago worm flour (Rhyinchophorus ferrugineus) reduced circulating NO levels in standard antimalarial therapy recipients.


2020 ◽  
Vol 20 ◽  
pp. 03006
Author(s):  
Deby Kania Tri Putri ◽  
Fadel Amirda ◽  
Hilman Muzadi ◽  
Amy Nindia Carabelly ◽  
Isyana Erlita ◽  
...  

Antibacterial compounds are generally produced from many herbal ingredients, but antibacterials may be produced from soil microbes, one of which is Actinomycetes. Actinomycetes are a soil bacterium capable of producing secondary metabolites in the form of antibacterial compounds. The antibacterial compound has the potential as an alternative antibacterial agent against oral microbes. One of the oral microbes that have a major role in the occurrence of caries is Streptococcus mutans and Lactobacillus acidophilus. This study aimed to analyze the antibacterial activity of Actinomycetes against the growth of S. mutans and L. acidophilus Actinomycetes extracts at the concentration of 0.625%, 1.25%, 2.5%, 5%, 10%, 20%, and 40% were respectively exerted as the treatment groups while 0.12% Chlorhexidine Gluconate (CHX) and aquadest as control. The measurement of antibacterial activity is done by looking at MIC and MBC. One Way ANOVA and Post Hoc Dunnets T3 test MIC and MBC values show p=0,000 (p <0.05). Actinomycetes extract at the concentration of 0.625% was able to inhibit the growth of S. mutans and L. acidophilus which was determined as the MIC and at the concentration of 40% as MBC of S.mutans and the concentration of 10% as MBC of L. acidophilus. There was an antibacterial activity of Actinomycetes to the growth of S. mutans and L.acidophilus.


2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Asro Abdi Firdaus ◽  
Setiawan . ◽  
Ferry Kriswandana

ABSTRACTMosquitoes are type of insect that acts as a disease vector. One of them is Culex sp mosquitos which their existence is many around us. In Indonesia there are still a lot of disease cases that caused by Culex sp mosquitos, one of them is filariasis disease. The efforts to control thecurrent population of Culex sp mosquitoes mostly use chemical insecticides. The control becomes difficult because of the resistance to chemical insecticides and adversely affects the environment. This study aims to determine the potential of jackfruit leaf extract as biolarvasida against Culex sp. The type of this study was post test onlycontrolled group design, using 625 larvae Culex sp instar III, divided into 5 groups (negative control group, 0.25% extract, 0.50% extract, 0.75% extract, extract and 1% extract). Observation was performed 24 hoursafter the treatment and counted the number of larvae death.The data were analyzed by Kruskal Wallis test followed by Post Hoc Mann-Whitney U test to determine the killing power of leafy leaf extractusing Probit analysis. The results of data analysis in this study were thedeath rate percentage of larvae after 24 hours treatment was 0% incontrol, 44% in 0.25%extract, 52% in0.50% extract, 60% in 0.75%extract,while in the extract group of 1%, the number of larval deaths was 72%. Itobtained results with significant differencein some treatment groups thatwere concentrations of 0.25% - 1%. Probit analysis showed that LC50 wasat 0.382% concentration.From results of the studyit can be concluded that jackfruit leaf extract(Artocarpus heterophyllus Lamk) was potential as Culex sp mosquitobiolarvacide. So it is necessary to conduct further research on the effectsof compounds that contained in other plant parts such as flowers, stemsand roots which will be expected to have a function asbiolarvasida.Keywords: Leaf Jackfruit, Biolarvasida, Mosquito Culex sp


2018 ◽  
Vol 5 (1) ◽  
pp. 45
Author(s):  
Yuvika Intan Ristian Putri ◽  
Muhammad Dian Firdausy ◽  
Niluh Ringga Woroprobosari

Background: Aplication of composites resin in relatively small amount as a restorative material often causing the material reaches its expiry date before all of the material can be used. Composites resin that have passed the expiry date would affect charateristic of the material. The aim of this study was to investigate surface hardness difference of composite resins based on material expiration date.Methods: This research was an experimental laboratory using post test only with control group design. Samples for this research were 27 divided into 3 groups based on its expiration date (2016, 2018 and 2019). The surface hardness was measured by Shore D Hardness. The result of this research were tested with One Way Anova and Post Hoc test LSD.Result: The lowest surface hardness result was measured on group 1 (81.02SHD) and the highest was group 3 (94.72 SHD). The result of One Way Anova test showed that there was signifcant differences in all groups (p<0.05). Post Hoc LSD test showed difference signifcantly between each groups.Conclusion: It can be concluded that there was signifcant difference of composite resins surface hardness based on material’s expiration date


Sign in / Sign up

Export Citation Format

Share Document