scholarly journals The Effectiveness Test Of Wound Healing Daun Gatal (Laportea Decumana) Against Mice (Mus Musculus L)

2021 ◽  
Author(s):  
Ratna Prabawati ◽  
Waskito Aji Suryo Putro ◽  
Yusnita La Goa ◽  
Lukman Hardia ◽  
Dyah Putri Utami
Keyword(s):  
Treatment Group ◽  
Formic Acid ◽  
Leaf Extract ◽  
Healing Rate ◽  
Pharmaceutical Product ◽  
Control Group ◽  
Leaf Sample ◽  
Exclusion Criteria ◽  
Group 3 ◽  
The Difference

Daun Gatal (Laportea spp) is one of the shrubs that are widely distributed in Papua from the coast to the mountains. Daun Gatal (Laportea spp) has been used for generations by the Papuan people as painkillers. Daun Gatal (Laportea spp) contains compounds monoxide, tryptophan, histidine, alkaloids, flavonoids, formic acid, and anthraquinones. This content is called "antacid" because it gives a sensation like being bitten by an ant. There are many itchy leaves in the village but often they are just left to dry, wither, die, and even be thrown away. The value of this leaf is very large if it is developed not only as an itchy leaf sheet but as a pharmaceutical product. (Simaremare, et al, 2019). This is supported by several research results stating that itchy leaf extract contains compounds of the alkaloid group, glycosides, steroids (Simaremare, 2014), also contains triterpenoid compounds and formic acid (Chrystomo, et al., 2016) and (Krisna and Santanina, 2019) which states that itchy leaves provide antibacterial activity. The type of research used is experimental research. The research design used was a randomized control group pretest and posttest design. The population in this study was white mice. The samples used in this study were white mice that had met the following inclusion and exclusion criteria. Inclusion criteria were female mice, bodyweight 20-30grams, age 2-4 months while the exclusion criteria. Included in the exclusion criteria in this study were mice that were sick or died in the study conditions. The results of the Extraction of Daun Gatal (Laportea Decumana), namely the itchy leaf sample that has been weighed at a concentration of 25% obtained from a mixture of itching leaf extract (25 grams) added with water (30 ml) produces 18.5 ml. Itchy leaf extract at a concentration of 50 % was obtained from a mixture of itchy leaf extract (50 grams) added with water (30 ml) to produce 20.1 ml. Gatak leaf extract at a concentration of 75% was obtained from a mixture of itching leaf extract (75 grams) added with water (30 ml) to produce 24.3 ml. The extraction method used is extracting the extract of itchy leaves. This method was chosen because the process is simple and does not involve heating so that it can prevent damage to chemical compounds that are not resistant to heating, especially flavonoids contained in itchy leaves. Based on the results of the data on the difference in wound diameter of mice, it showed that Treatment Group 1 with 25% itching leaf extract and Treatment Group 2 with 50% itching leaf extract almost had the same healing rate. Meanwhile, Treatment Group 3 with 75% itching leaf extract had the fastest healing rate among other concentrations. In contrast to Treatment Group 3, the control group had a much longer healing rate among other concentrations.

scholarly journals Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis

2010 ◽  
Vol 8 (2) ◽  
pp. 47-51
Author(s):  
NUR AMALINA ◽  
SUYATMI SUYATMI ◽  
ENDANG LISTYANINGSIH SUPARYANTI
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Leaf Extract ◽  
Study Groups ◽  
Control Group ◽  
High Concentration ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
The Difference

Amalina N, Suyatmi, Suparyanti EL. 2010. Effect of beluntas (Pluchea indica) leaf extract on mice spermatogenesis. Biofarmasi 8: 47-51. This research aimed to determine the effect of beluntas (Pluchea indica L.) leaf extract in habiting spermatogenesis. Flavonoids can inhibit aromatase enzyme that function to catalyze the conversion of androgens into estrogen so that the level of the testosterone hormone will increase. The high concentration of testosterone will affect on the feedback to the pituitary, that not releasing FSH and LH, so it will inhibit spermatogenesis. This research is an experimental research with post-test only controlled group design method, using male mice, 2-3 months age, weight 20-30 g, as many as 24 mice divided into 4 groups. The first group was a control group, the second group as treatment group I that given with 1.4 mg/20 g body weight beluntas extract, the third group as treatment group II that given with 2.8 mg/g body weight beluntas extract. The fourth group as treatment group III that given with 5.6 mg/g body weight beluntas extract. The treatment was given for 10 days after that the testes of mice were taken and from each testicle was made 3 slices and from each slice the most representative seminiferous tubule was taken for spermatid cells. So, each mice had 18 units of data would be analyzed. The data obtained were analyzed with Anova to determine the significant differences before and after the treatment of extract and to be compared the difference between four groups with Dunnet T3 test to determine the difference between each group. Based on the statistical test results with Dunnet T3, it showed a significant difference between the fours study groups, except between treatment groups I and II. This might be due to the effectiveness of the two doses equally so that by doubling the dose, it did not give a doubling effect on decreasing spermatids. The provision of beluntas leaf extract can cause a decrease in the number of spermatid cells in mice. The average spermatid cells number decreased with increasing beluntas leaf extract dose. The best dose for reducing the number of spermatids in this study was the dose in the treatment group III that was 5.6 mg/g body weight.

Clinical Study of Qingpeng Ointment Treatment of Shoulder-Hand Syndrome After Cerebral Hemorrhage During the Rehabilitation Period

2020 ◽  
Vol 23 ◽  
Author(s):  
Ruihuan Pan ◽  
Shanshan Ling ◽  
Haodong Yang ◽  
Yan Huang ◽  
Lechang Zhan ◽  
...  
Keyword(s):  
Treatment Group ◽  
Cerebral Hemorrhage ◽  
Clinical Medicine ◽  
External Rotation ◽  
Statistical Significance ◽  
Hand Function ◽  
Tibetan Medicine ◽  
Controlled Study ◽  
Control Group ◽  
The Difference

Background: Shoulder-hand syndrome (SHS) refers to a syndrome causing sudden edema, shoulder pain and limited hand function. Qingpeng ointment, a kind of Tibetan medicine, can reduce swelling, relieve pain, tonify stagnation and clear the meridians, which is consistent with the pathological mechanism of SHS after stroke. Therefore, if clinical trials can be used to explore the effectiveness of Qingpeng ointment for treatment of poststroke SHS and promote its application in clinical medicine, this is of specific significance for the treatment of poststroke SHS. Objective: To investigate the clinical efficacy and safety of Qingpeng ointment in the treatment of poststroke SHS. To provide an objective basis for a better therapeutic treatment for poststroke SHS． Method: A prospective, randomized, controlled study was conducted. This study recruited 120 patients with poststroke SHS who met the inclusion criteria. They were randomized into the treatment group and the control group, with 60 patients allocated to each group. The treatment group received routine medical treatment and rehabilitative care after using the Qingpeng ointment, while the patients in the control group received only routine treatment without the ointment. All patients received clinical assessment with the Visual Analogue Scale (VAS), measurement of the range of motion (ROM) of the upper-limb joints, the Fugl-Meyer Assessment of Upper Extremity (FMA-U) and the Modified Barthel Index Score (MBI) before and after the whole treatment. Results: After 4 weeks of treatment, the VAS scores of both groups were decreased significantly (P＜0.05), and the difference between the two groups was statistically significant (P < 0.05). There is no statistical significance for the difference between the treatment group and control group in terms of the FMA-U and MBI scores and the forward bend, backward, outstretch, external rotation and pronation angles after treatment. The increases in the values of VAS, FMA-M and MBI in the treatment group were greater than those in the control group, and the difference was statistically significant (P < 0.05). The increases in the values of the forward bend, outreach and external rotation angles in the treatment group were greater than those in the control group, and the difference was statistically significant (P < 0.05). Conclusion: The treatment group showed better results than the control group in terms of the relief of pain symptoms, the improvement of motor function and the improvement of the activities of daily living for patients with shoulder-hand syndrome after cerebral hemorrhage. Qingpeng ointment is effective and safe in treating poststroke SHS.

Clinical Application of Scalp Cluster Needling Combined with Bobath Technique in Upper Limb Spastic Paralysis After Stroke of Vertebral Artery Type

2021 ◽  
Author(s):  
Aimin Gong ◽  
Mengjie Zeng ◽  
Zhiquan Wu
Keyword(s):  
Treatment Group ◽  
Acupuncture Therapy ◽  
Vertebral Artery ◽  
Upper Limb ◽  
Training Group ◽  
Control Group ◽  
Upper Limbs ◽  
Spastic Paralysis ◽  
Rehabilitation Training ◽  
The Difference

To observe the difference in clinical effects of scalp-point cluster acupuncture combined with rehabilitation training in treating spastic paralysis of upper limbs after stroke. Using a randomized controlled design, 96 patients with upper limb spastic paralysis after stroke were randomly divided into two groups: treatment group (scalp acupuncture plus rehabilitation training group 48 cases), control group (rehabilitation training group 48 cases). After 2 courses of treatment, it was judged by observing clinical efficacy evaluation, Ashworth classification, and Fugl-Meyer (FMA) score. The total effective rate was 91.7% in the treatment group and 68.7% in the control group; the difference between the two groups was statistically significant (P <0.05). Before treatment, the difference in Ashworth classification between the two groups was not statistically significant (P> 0.05); after treatment, the difference between the two groups was statistically significant (P <0.01). After treatment, the difference between the two groups was statistically significant (P＜0.05). The Fugl-Meyer (FMA) scores of the two groups of patients before treatment were comparable (P>0.05), and there were significant differences between the two groups after treatment (P <0.05). The difference of Fugl-Meyer (FMA) scores between the two groups was statistically significant (P < 0.01). Scalp cluster acupuncture therapy is more effective than traditional acupuncture therapy alone in treating vertebral artery type cervical spondylosis. Scalp cluster acupuncture combined with Bobath technique is effective in treating spastic paralysis of upper limbs after stroke, and it is worthy of clinical application.

scholarly journals Hibiscus sabdariffa Aqueous Leaf Extract Reverses Hematological Alterations in Phenylhydrazine Anemic Wistar Rats

2020 ◽  
pp. 30-38
Author(s):  
E. B. Umoren ◽  
T. A. Kolawole ◽  
I. Wopara ◽  
O. G. Adebayo ◽  
B. Ben-Azu ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Leaf Extract ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Hibiscus Sabdariffa ◽  
Aqueous Leaf Extract ◽  
Group 3 ◽  
Group 2 ◽  
Hb Concentration

Background: The extract of Hibiscus sabdariffa commonly known as Sobo is widely consumed for its nutritional and health benefits. This study investigated the effect of aqueous leaf extract of  H. sabdariffa on anemic condition caused by phenylhydrazine in rats. Materials and Methods: Thirty (30) rats used for this study were divided into three groups of 10 rats each. Group 1 received distilled water and served as control. Group 2 received phenylhydrazine (40 mg/kg, i.p.). Group 3 was treated with phenylhydrazine (40 mg/kg, i.p.) 30 minutes prior to administration of (200 mg/kg, p.o.) aqueous extract of H. sabdariffa (Sobo) once daily for 14 days. At the end, 2 ml blood samples were collected through cardiac puncture into clean sample bottles containing ethylenediamine tetra acetic acid (EDTA) for hematological analyses. Results: Sobo significantly increased (P<0.05) hemoglobin (Hb) concentration and packed cell volume (PCV) in the phenylhdrazine-treated rats. Also, Sobo significantly (P<0.05) increased total white blood cell (TWBC) in phenylhydrazine administered rats. However, the extract did not produce any significant effect on mean corpuscular hemoglobin concentration (MCHC) relative to control and anemic groups. Conclusion: The findings from this study revealed that the aqueous extract of H. sabdariffa demonstrates anti-anemic effect in rats treated with phenylhydrazine, suggesting its ethno-pharmacological beneficial effect in anemic conditions.

scholarly journals Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Leaf Extract ◽  
The Body ◽  
Control Group ◽  
Bb Rat ◽  
Toxic Dose ◽  
Experimental Animals ◽  
Significant Difference ◽  
Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

scholarly journals OHIS Differences on Primary School Students in Relation to Little Dentist Training

2021 ◽  
Author(s):  
Asep Arifin Senjaya ◽  
Ni Made Sirat ◽  
I Nyoman Wirata ◽  
Ni Ketut Ratmini
Keyword(s):  
Treatment Group ◽  
Primary School ◽  
Oral Hygiene ◽  
Dental Health ◽  
Control Group ◽  
Primary School Students ◽  
School Students ◽  
Significant Difference ◽  
Before And After ◽  
The Difference

Dental healthcare is very important since dental and oral hygiene should be maintained. Statistics showed that more than 80% of children in developed and developing countries suffer from dental disease. This study aimed to see the safety of OHIS (Oral Hygiene Index Simplified) in primary school students who got and did not get little dentist cadre training in Bangli Regency in 2019. The study was done in an experimental design: pre and post-test with control design, which was conducted in August-September 2019. The sample in this study is 366 students. The difference in OHIS scores before and after treatment in the control group and treatment group was carried out by the bivariate Mann Whitney U Test. The results of the study showed that before dental health training was conducted, there were 54.3% of primary school students in the treatment group with good OHIS score criteria, and after the training was carried out as many as 98.4% of the students in the treatment group had a good OHIS. Additionally, prior to the training, 57.5% of the control group had fair OHIS criteria score. Then, after the training was carried out, 73.7% of the control group had good OHIS score. Hence, the study concluded that there was a significant difference in the OHIS of primary school students who got little doctor training prior to and after the training. Also, there was a significant difference in the OHIS of the students who did not get such treatment before and after the training.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

Effectiveness of patching traumatic eardrum perforations with lens cleaning paper via an otoscope

2017 ◽  
Vol 131 (9) ◽  
pp. 805-808 ◽  
Author(s):  
K Yan ◽  
M Lv ◽  
E Xu ◽  
F Fan ◽  
Y Lei ◽  
...  
Keyword(s):  
Healing Rate ◽  
Clinical Effect ◽  
Healing Time ◽  
Control Group ◽  
Closure Rate ◽  
The Difference

AbstractObjective:To study the clinical effect of lens cleaning paper patching on traumatic eardrum perforations.Methods:A total of 122 patients were divided into 2 groups, of which 56 patients were treated with lens cleaning paper patching and 66 acted as controls. The closure rate and healing time were compared between the two groups.Results:The healing rate of small perforations was 96.4 per cent (27 out of 28) in the patching group and 90 per cent (27 out of 30) in the control group. The difference was not statistically significant (p > 0.05). The healing rate of large perforations was 89.3 per cent (25 out of 28) and 80.6 per cent (29 out of 36) in the two groups, respectively. The difference was statistically significant (p < 0.05). The healing time of large perforations was shorter in the patching group than in the control group (p < 0.01).Conclusion:Patching with lens cleaning paper under an endoscope can accelerate the closure of large traumatic eardrum perforations.

scholarly journals Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats

2020 ◽  
Vol 31 (1) ◽  
pp. 49
Author(s):  
Festi Artika Sari ◽  
Willy Sandhika ◽  
Tri Hartini Yuliawati
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Leaf Extract ◽  
Inflammatory Cell ◽  
Negative Control ◽  
Inflammatory Cell Infiltration ◽  
Male Rats ◽  
Cell Infiltration ◽  
Ocimum Sanctum ◽  
Control Group

<p class="ISIABSTRAKINGGRIS">Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value &lt;0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.</p>

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