scholarly journals Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats

2020 ◽  
Vol 31 (1) ◽  
pp. 49
Author(s):  
Festi Artika Sari ◽  
Willy Sandhika ◽  
Tri Hartini Yuliawati
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Leaf Extract ◽  
Inflammatory Cell ◽  
Negative Control ◽  
Inflammatory Cell Infiltration ◽  
Male Rats ◽  
Cell Infiltration ◽  
Ocimum Sanctum ◽  
Control Group

<p class="ISIABSTRAKINGGRIS">Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value &lt;0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.</p>

scholarly journals The Effect of Salam Leaf (Syzygium polyanthum [Wight] Walp.) Extract on Inflammation in Rat Model of Acute Coronary Syndrome

2020 ◽  
Vol 8 (A) ◽  
pp. 374-377
Author(s):  
Refli Hasan ◽  
Dharma Lindarto ◽  
Gontar Alamsyah Siregar ◽  
Zulfikri Mukhtar
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Macroscopic Appearance ◽  
Coronary Syndrome

AIM: The aim of the study was to determine the effect of Salam leaf extract on regenerating islet-derived protein 3 β (Reg3β) and C-reactive protein (CRP) in cardiomyocyte of rat model of ACS. METHODS: Thirty-six male Wistar rats were divided into three groups: Negative control group, positive control group (ACS-induced group), and treatment group (ACS-induced group with Salam leaf administration). Macroscopic appearance and histopathology examination were done to ensure the occurrence of infarction. Reg3β and CRP levels were measured and compared between the positive control group and treatment group. RESULTS: There were significantly lower Reg3β and CRP levels in the treatment group than in the positive control group from day 4 to day 14. CONCLUSION: These data suggest that a Salam leaf extract can improve inflammation by decreasing Reg3β and CRP levels in rat model of ACS.

scholarly journals Topical Application of Sadat-Habdan Mesenchymal Stimulating Peptide (SHMSP) Accelerates Wound Healing in Diabetic Rabbits

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Abdulmohsen H. Al-Elq ◽  
Mir Sadat-Ali ◽  
Mohamed Elsharawy ◽  
Ibrahim Al-Habdan ◽  
Fatin Othman Al-Aqeel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Topical Application ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Control Group ◽  
Collagen Deposition ◽  
Peptide Group ◽  
Diabetic Rabbits

Objective. Diminished wound healing is a common problem in diabetic patients due to diminished angiogenesis. SHMSP was found to promote angiogenesis. The present study was carried out to examine the effect of this peptide in healing of wounds in diabetic rabbits.Materials and Methods. Twenty male New Zealand rabbits were used in this study. Diabetes mellitus was induced and the rabbits were randomly divided into two equal groups: control group and peptide group. A-full thickness punch biopsy was made to create a wound of about 10 mm on the right ears of all rabbits. Every day, the wound was cleaned with saline in control groups. In the peptide group, 15 mg of SHMSP was applied after cleaning. On day 15th, all animals were sacrificed, and the wounds were excised with a rim of 5 mm of normal surrounding tissue. Histo-pathological assessment of wound healing, inflammatory cell infiltration, blood vessel proliferation, and collagen deposition was performed.Results. There were no deaths among the groups. There was significant increase in wound healing, blood vessel proliferation and collagen deposition, and significant decrease in inflammatory cell infiltration in the peptide group compared to the control group.Conclusion. Topical application of SHMSP improves wound healing in diabetic rabbits.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

scholarly journals Prospect of Bay Leaf (Syzygium Polyanthum) Extract in Reducing Ischemia and Inflammation in Animal Model of Myocardial Infarction: An Experimental Study

2020 ◽  
Author(s):  
Refli Hasan ◽  
Gontar Alamsyah Siregar ◽  
Dharma Lindarto
Keyword(s):  
Myocardial Infarction ◽  
Experimental Study ◽  
Animal Model ◽  
Treatment Group ◽  
Leaf Extract ◽  
Immune Cell ◽  
Statistical Significance ◽  
Cell Infiltration ◽  
Control Group ◽  
Immune Cell Infiltration

Introduction: Bay leaf (Syzygium polyanthum) was thought to have potential to treat cardiovascular disease traditionally. Myocardial Infarction (MI) characterised by sudden blockage of coronary artery due to atherosclerosis rupture and following MI, several changes could happen such as expression of Hypoxia-Inducible Factor (HIF) 1α, immune cell infiltration, cytokine release, and fibrosis. Aim: To study the effect of bay leaf extract on HIF-1α, immune cell infiltration, IL-10 level, and fibrosis in animal model of MI. Materials and Methods: An Experimental study using animal model of MI was conducted in December 2019. Thirty two Wistar rats were surgically manipulated to have MI. Rats were then divided into two groups: treatment group (with bay leaf extract administration) and control group. Neutrophil and macrophage distribution, collagen (fibrosis) distribution, and HIF-1αexpression were examined in infarcted muscle. Additionally, serum was taken to measure IL-10 level. Observation was conducted on day 1, day 4, day 7, day 14 after MI episode. Independent t-test was used to compare serum IL-10 level between groups. Statistical significance was set at p<0.05. Results: Lower HIF-1α expression was seen in treatment group since day 1, showing better response to ischemia. As for inflammation, decreased neutrophil distribution, increased macrophage distribution was observed in treatment group. Significant increases in IL-10 level were also noticed since day 1 in treatment group (p<0.05 in all observation day). Lower fibrosis was also noticed from day 1 in treatment group. All these effects were further seen in day 4, day 7, and day 14. Conclusion: Bay leaf extract has potential in reducing ischemia, inflammation, and cardiac fibrosis in animal model of MI.

scholarly journals The Hepatoprotective Effects of Basil Leaf (Ocimum sanctum L.) Extract on Paracetamol Induced Liver Damage in Male Rat

2021 ◽  
Vol 7 (2) ◽  
pp. 444-450
Author(s):  
Ronaldo Panggabean ◽  
Nofita ◽  
Ade Maria Ulfa
Keyword(s):  
Liver Damage ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Male Rat ◽  
Ocimum Sanctum ◽  
Sprague Dawley ◽  
Percolation Method ◽  
Sprague Dawley Rats

Basil leaf have antioxidants such as flavonoids, so it is thought to have a hepatoprotective effect. This study aims to investigate the effect of basil leaf extract on SGOT and SGPT levels in male rats induced by paracetamol. Basil leaf extract was carried out by the percolation method using ethyl acetate solvent, Some 20 male sprague dawley rats were randomly divided into 5 groups. Basil leaf extract (400 mg/kgBB and 600 mg/kgBB) and sylimarin (100 mg/kgBB) were carried out every day for 28 days, paracetamol was induced 24 hours after giving the last day of basil leaf extract. The parameters measured were SGOT and SGPT level to assess the effect of basil leaf extract on liver damage caused by paracetamol. The results showed that basil leaf extract (400 mg/kgBB dan 600 mg/kgBB) showed that the activities of SGOT and SGPT levels were statistically significant (p<0,05) to negative control. Basil leaf extract shows the effect of hepatoprotector on liver induced by paracetamol, however the effect given was not able to equate with positive control.

scholarly journals Morphological changes in the ventilated lung after thoracic surgery

2021 ◽  
Vol 27 (2) ◽  
pp. 11-15
Author(s):  
A.V. Sydiuk ◽  
O.Ye. Sydiuk ◽  
V.O. Kropelnytskyi ◽  
A.S. Klimas
Keyword(s):  
Thoracic Surgery ◽  
Inflammatory Cell ◽  
Morphological Changes ◽  
Lung Ventilation ◽  
Inflammatory Cell Infiltration ◽  
Lung Damage ◽  
Cell Infiltration ◽  
Control Group ◽  
Study Group ◽  
Alveolar Wall

There are many studies of single lung ventilation (SLV), which are mostly limited to reducing lung damage by changing ventilation strategies or comparing differences in lung damage caused by different lung isolation devices. There is no study comparing the morphological changes of ventilated lungs using different strategies of artificial lung ventilation. The aim of the study was to examine pathomorphological changes in the ventilated lung during thoracic surgery using SLV. A randomized study was performed on 40 patients who underwent thoracic surgery using SLV. After signing the informed consent, the patients were divided into two groups. In the control group (40 patients) with ventilation “by volume” (VCV), in the study group – ventilation “by pressure” (PCV) with the addition of PEEP 5 mm. During surgery in the thoracic cavity with the help of SLV performed transbronchial biopsy of the parenchyma of the ventilated lung to study the pathomorphological changes after ventilation with different modes. The biopsy was performed using a bronchoscope, which was inserted through the endotracheal tube into the lung, opposite the side of the operation (after the end of SLV and “inclusion” of the collapsed lung). The morphological changes caused by the ventilator were investigated. Pathomorphological examination of the non-collapsed lung (which participated in gas exchange during SLV) was as follows: the control group found significant changes in the alveolar wall with its edema, thickening of the interstitial lung, vascular occlusion, severe inflammatory cell infiltration and damage to alveolar structures. The alveoli collapsed and disappeared. The alveolar structures of the study group were better than the control group: pulmonary interstitial and alveolar exudates, as well as inflammatory cell infiltration were significantly reduced compared to those in the control group. The results of the study suggest that the use of PCV with “moderate” PEEP can significantly improve oxygenation and reduce acute ventilatory injury of the lungs compared to VCV during SLV.

scholarly journals Administration of red rosella’s petals extract (Hibiscus sabdariffa) increase level of superoxide dismutase (SOD) wistar rats which induced by repeteadly heated cooking oil

2017 ◽  
Vol 4 (2) ◽  
pp. 13 ◽  
Author(s):  
Novera Herdiani ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Wistar Rats ◽  
Saturated Fatty Acids ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
Cooking Oil

Background:  repeatedly heated cooking oil is cooking oil that is used many times, had saturated fatty acids containing free radicals. Red rosella petals extract contains antioxidants to minimize the effects of repeatedly heated cooking oil that can raise the levels of SOD. The aim of this study was to analyzed the effect of red rosella’s petals extract against oxidative stress of Wistar rats were induced by repeatedly heated cooking oil with examine levels of  serum SOD.This research was conducted True Experimental Laboratory with a completely randomized design (CRD) and use the post test. The sample consisted of 24 male rats were divided 4 groups: negative control (fed with standard diet); positive control (fed with standard diet + administered by repeatedly heated cooking oil as much as 2.1 ml / kg bw); treatment group dose I (fed with standard diet + administered by red rosella extract dose 540 mg / kg bw + repeatedly heated cooking oil as much as 2.1 ml / kg bw), and treatment group dose II (fed with standard diet + administered by red rosella extract dose 810 mg / kg bw + repeatedly heated cooking oil as much as 2.1 ml / kg bw). Analysis of data using statistical test One Way ANOVA and Tukey HSD (α = 0.05).Results: There were significant differences (p = 0.000) between groups. Red rosella extract treatment group dose 810 mg / kg bw and 540 mg / kg bw were significantly different from the positive control (p = 0.000). Red rosella extract group dose 540 mg / kg bw was significantly different from the negative control (p = 0.000). Red rosella extract group dosage of 810 mg / kg bw was not differ significantly from the control group (p = 0.171).Conclusion: The red rosella’s petals extract dose of 810 mg / kg bw and dose of 540 mg / kg bw were able to prevent oxidative stress. Optimal deose of red rosella petals extract dose of 810 mg / kg bw was the most effective, because the higher antioxidant activity (67.33%) at dose 810 mg / kg bw dose than 540 mg / kg bw.

Abstract TP279: Brain-Derived Microparticles Mediate Neuroinflammation After Stroke

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Zhili Chen ◽  
Michael Chopp ◽  
Alex Zacharek ◽  
Wei Li ◽  
Poornima Venkat ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Neurological Deficits ◽  
Cell Infiltration ◽  
Control Group ◽  
Lesion Volume ◽  
Inflammatory Factor ◽  
Ischemic Brain ◽  
Factor Expression ◽  
Anti Inflammatory

Background and Purpose: Microparticles (MPs, ~ size between 0.1-1mm) are lipid encased containers, and are involved in intercellular communication and regulate inflammation. Stroke increases brain derived MP (BDMP) secretion which induces neuroinflammation. Milk fat globule-EGF factor-8 (MFGE8) promotes apoptotic cell clearance and limits pathogenic antigen cross presentation. In this study, we investigate whether BDMP affects stroke-induced neuroinflammation; whether MFGE8 treatment reduces stroke or BDMP-induced neuroinflammation and improves functional outcome after stroke. Method: 1) BDMPs were extracted from ischemic brain 24h after dMCAo by ultracentrifugation. 2) Adult (8 months) male C57BL/6J mice were subjected to dMCAo and were injected via tail vein 3h after stroke with: A) PBS (n=5/group); B) +BDMP(1.5х10 8 ,n=6/group); C)+MFGE8 (Lactadherin, 400ug/kg, n=5/group); D) +BDMP+MFGE8 (n=6/group). A battery of neurological function outcomes and immunostaining were performed. Blood plasma was used for Western blot assay. Result: 1) Compared with the Stroke+PBS control group, Stroke+BDMP significantly increases inflammatory factor expression in the circulation, increases lesion volume, neurological deficits, blood brain barrier (BBB) leakage, microglial activation, and inflammatory cell infiltration (CD45, microglia/macrophage, Neutrophils) and inflammatory factor (TNFα, IL6, IL1β) expression in brain, and increases axon/white matter (WM) damage; 2) Compared to Stroke+PBS and Stroke+BDMP groups, Stroke+MFGE8 and Stroke+BDMP+MFGE8 mice exhibited significantly improved neurological outcome; decreased lesion volume and BBB leakage, reduced axon/WM damage, and decreased inflammatory cell infiltration and inflammatory factor expression in the ischemic border, respectively. MFGE8 treatment significantly increased anti-inflammatory factor (IL10) expression in ischemic brain, and decreased IL1β expression in circulation compared to Stroke+PBS and Stroke+BDMP groups, respectively. Conclusion: BDMP increases neuroinflammation and induces worse brain damage after stroke. MFGE8 treatment reduces stroke and BDMP-induced neurological deficits possibly via its anti-inflammatory effects.

Stromal Cell-Derived Factor (SDF)-1-Pretreated Bone Marrow Mesenchymal Stem Cells (BMSCs) Ameliorates Acute Kidney Injury in Mice

2021 ◽  
Vol 11 (7) ◽  
pp. 1255-1262
Author(s):  
Xiaohui Dong ◽  
Xifeng Lv
Keyword(s):  
Inflammatory Cell ◽  
Kidney Injury ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Control Group ◽  
Serum Cytokines ◽  
Marrow Mesenchymal Stem Cells ◽  
Stromal Cell Derived Factor ◽  
Group 2 ◽  
Experimental Group

To explore the effects of stromal cell-derived factor (SDF-1) pretreatment of bone marrow mesenchymal stem cells (BMSCs) on acute kidney injury (AKI) in mice. BMSCs were cultured and treated with SDF-1 to detect osteogenic and adipogenic ability. Cisplatin (20 mg/kg) was used to establish AKI model and then divided into blank group, control group 2 (BMSCs injection), and experimental group (intraperitoneal injection of BMSCs treated with SDF-1 (80 ng/ml)) followed by analysis of serum cytokines (Toll like receptor 4 (TLR4), tumor necrosis factor-α (TNF-α), and interleukine-6 (IL-6)) by enzyme linked immunosorbent assay (ELISA). In cultured BMSCs, positive rates of CD29, CD44, CD45, and CD11b were 98.2%, 97.6%, 2.5% and 2.1%, respectively. When the concentration of SDF-1 was within 80 ng/mL, the chemotaxis and proliferation ability was dose-dependent (p < 0.05). SDF-1 pretreatment did not affect BMSCs adipogenic and osteogenic abilities. The creatinine and serum cytokines (TLR4, TNF-α, and IL-6) level in experimental group showed statistical significance (p < 0.05). At 24 h, thrombosis and tubular dilatation in the mesangial region of control group 2 and experimental group under light microscope were similar without difference of inflammatory cell infiltration and fibrosis. At 72 h, the glomerular mesangium widened in control group 2 with focal segmental sclerosis, renal tubules dilated, and protein casts and inflammatory cell infiltration and fibrosis. Experimental group showed a small amount of cell proliferation in the glomerular mesangium with few inflammatory cell infiltration and fibrosis. SDF-1 can enhance the migration and proliferation activity of BMSCs, reduce extracellular matrix precipitation, improve renal fibrosis, and alleviate AKI.

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