Effect of Paclobutrazol on Off-Season Flower Induction in Acid Lime (Citrus aurantifolia Swingle) Landraces under Chitwan Condition

The experiment was conducted to assess the effectiveness of paclobutrazol in inducing off-season flowering in two Nepalese and one Indian commercial landraces of acid lime during 2002 to 2003 at Rampur, Chitwan, Nepal. The experiment was laid out in split-plot design with landraces as main plot and dates of application of paclobutrazol as sub plot factors. Fourteen month-old seedlings of landraces planted on 28 May 2001 were soil drenched with paclobutrazol (25%) @ 5 ml per tree at the collar region of the tree on four different dates at 50 days interval beginning from 17 July to 17 December, 2002. Paclobutrazol applied on 17 July was the most effective in inducing early flowering at fourth week of December, which was 70 days ahead of normal flowering date. The subsequent applications on September, October and December also advanced flowering time by 59, 41 and 32 days, respectively. The earlier (July) application of paclobutrazol was superior among the treatments under Chitwan condition to induce and advance early flowering for offseason market. Key words: Cultar, sourlime, germplasm J. Inst. Agric. Anim. Sci. 26:87-92 (2005)

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PERTUMBUHAN TANAMAN DAN KANDUNGAN TOTAL FILANTIN DAN HIPOFILANTIN AKSESI MENIRAN (Phyllanthus sp. L) PADA BERBAGAI TINGKAT NAUNGAN

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v17n1.2011.25-31 ◽

2020 ◽

Vol 17 (1) ◽

pp. 25

Author(s):

EVA OKTAVIDIATI ◽

M.A. CHOZIN ◽

N. WIJAYANTO ◽

M. GHULAMAHDI ◽

L.K. DARUSMAN

Keyword(s):

Plant Growth ◽

Key Words ◽

Family Member ◽

Plant Height ◽

Accession Number ◽

Active Constituent ◽

Main Plot ◽

Number Of Branches ◽

Plot Design

ABSTRAKMeniran adalah anggota dari famili Euphorbiaceae. Lignan, berupafilantin dan hipofilantin yang ada di dalam tanaman, dilaporkan sebagaiagen hepatoprotektif dalam terapi pengobatan yang utama. Eksplorasitelah dilakukan terhadap 13 aksesi meniran pada berbagai tipe habitat yangberbeda di Kabupaten Bangkalan dan Gresik, Provinsi Jawa Timur.Penelitian ini bertujuan untuk melihat pengaruh dari aksesi dan tarafnaungan terhadap pertumbuhan dan kandungan filantin dan hipofilantintanaman meniran (Phyllanthus sp. L). Penelitian dilakukan di KebunPercobaan IPB di Babakan Sawah Baru, Bogor, Jawa Barat denganketinggian tempat 250 m dml dari Maret 2009 sampai September 2009.Percobaan disusun dalam rancangan petak terbagi dengan 3 kali ulangan.Petak utama adalah 3 taraf naungan (N) terdiri atas : 0% (N0), 25%naungan (N1) dan 50% naungan (N2). Anak petak adalah 13 aksesimeniran (A) terdiri atas : A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, A11,A12, dan A13 berasal dari Bangkalan dan Gresik. Hasil penelitianmenunjukkan bahwa naungan 50% meningkatkan tinggi tanaman danmenurunkan jumlah daun majemuk. Terjadi interaksi naungan dan aksesiterhadap jumlah cabang. Uji Duncan menghasilkan 3 kelompok aksesiyang mempunyai respon berbeda terhadap naungan. Aksesi nomor 6 dan 7merupakan aksesi yang menunjukkan pertumbuhan dan produksi biomassayang lebih besar dibandingkan aksesi lainnya. Aksesi nomor 7 mempunyaikandungan total filantin dan hipofilantin tertinggi, karena pengaruhnaungan dapat menurunkan kandungan total filantin dan meningkatkankandungan total hipofilantin.Kata kunci : Phyllanthus sp. L., filantin, hipofilantin, naungan, aksesiABSTRACTPlant growth and total phyllanthin and hypophyllanthincontents of Phyllanthus sp. L accession on variousshading levelsMeniran (Phyllanthus sp. L) is family member of Euphorbiaceae.The lignan, consisting of phyllanthin and hypophyllanthin in the plant, wasreported as therapeutically active constituent and serve as hepatoprotectiveagent. The objective of this research was to investigate the effect ofshading intensities on plant growth and phyllanthin and hypophyllanthincontents of Phyllanthus sp. accessions. The experiment was arranged insplit plot design with three replicates. The main plot was shading intensityconsisting of 0% (N0), 25% (N1), and 50% (N2) shades. The sub plot wasof Phyllanthus sp. accessions (A) consisting of A1, A2, A3, A4, A5, A6,A7, A8, A9, A10, A11, A12, and A13 taken from Bangkalan and Gresik.The results showed that 50% shade increased plant height but decreasednumber of leaves. Interaction between shading intensity and accessiongave significant effect on number of branches. Referring to their responseson shades, all accessions were divided into 3 groups by DMRT. Biomassproductions of accessions number 6 and 7 were greater than the otheraccessions. Accession number 7 was the highest in phyllanthin andhypophyllanthin contents where the shading reduced the phyllanthin butincreased the hypophyllanthin contents.Key words: Phyllanthus sp., phyllanthin, hypophyllanthin, shading,accessions

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Pengaruh Aplikasi GA3 dan Pemupukan NPK Terhadap Keragaan Tanaman Cabai sebagai Tanaman Hias Pot

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.4.3.157-166 ◽

2015 ◽

Vol 4 (3) ◽

pp. 157

Author(s):

Yusnita Sari ◽

Ketty Suketi

Keyword(s):

Key Words ◽

Plant Height ◽

Consumer Preferences ◽

Block Design ◽

Randomized Complete Block Design ◽

Split Plot ◽

Main Plot ◽

Npk Fertilizer ◽

Plot Design

ABSTRACTThe objected of this experiment was to know the dose of NPK fertilizer and most appropriate application of GA3 to increase ornamental pepper quality. The experiment has been done from May until August 2009 at Leuwikopo greenhouse, IPB. The experiment was arranged in Split Plot Design within Randomized Complete Block Design. Main Plot factors was application of GA3 (0 ppm, 100 ppm, and 200ppm) and sub plot was dosage of NPK fertilizer (0 g polybag-1, 1.5 g polybag-1, 3 g polybag-1, and 6 g polybag-1). The results showed that GA3 100 and 200 ppm increased the plant height, number of nodes of plant, and elongated the internode of stem, but the generative growth of plant become pursued. NPK fertilizer 6 g polybag-1gave the lowest of plant height and have yielded a few of flower and fruit. Based on test of consumer preferences, the best appearance was combination without treatment of GA3 and fertilization NPK 3 g polybag-1.Key words : Ornamental pepper, GA3, NPK ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui dosis pupuk NPK dan konsentrasi yang paling tepat dari aplikasi GA3 untuk meningkatkan kualitas tanaman Lada hias. Percobaan penelitian dilakukan dari bulan Mei sampai dengan Agustus 2009 di rumah kaca kebun percobaan Leuwikopo, IPB. Percobaan menggunakan rancangan Split plot acak lengkap. Plot utama adalah aplikasi GA3 (0, ppm 100 ppm, dan 200 ppm) dan anak petak adalah dosis pupuk NPK (0 g polybag-1, 1.5 g polybag-1, dan 6 g polybag-1). Hasil penelitian menunjukkan bahwa perlakuan GA3 100 dan 200 ppm dapat meningkatkan tinggi tanaman, jumlah node dan internode pada caban. Pupuk NPK dengan taraf aplikasi 6 g polybag-1 menunjukkan hasil yang terendah pada tinggi tanaman, dan jumlah bunga serta buah yang lebih sedikit. Berdasarkan uji preferensi konsumen, yang paling disukai adalah Lada dari perlakuan tanpa GA3 dan dengan penambahan pupuk NPK 3 g polybag-1.Kata kunci: GA3, NPK, Tanaman Lada NPK

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Cytokinin for Growth and Productivity of Polyploid Chrysanthemum on Third Generation

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/748/1/012031 ◽

2021 ◽

Vol 748 (1) ◽

pp. 012031

Author(s):

Ai Komariah ◽

Elly Roosma Ria ◽

Endah Islamiyati ◽

Noertjahyani ◽

Nunung Sondari ◽

...

Keyword(s):

Growth Rate ◽

Experimental Design ◽

Flowering Time ◽

Third Generation ◽

Great Demand ◽

The Third ◽

Best Response ◽

Main Plot ◽

Different Levels ◽

Plot Design

Abstract Chrysanthemum (Chrysanthemump) is a one of the most popular cutting flower commodities in Indonesia. The beauty of colors and variations in the shape of flowers and the low level of withering cause Chrysanthemum to be in great demand. The polyploid chrysanthemum has some superior properties but the growth rate and flowering time is slow due to colchicine. The study aimed to determine the influence of the concentration of the cytokinins in the growth and productivity of chrysanthemum polyploid genotypes. The experimental design as used Split Plot Design consists of 2 factors and 2 replications. The first factors were concentration of the cytokinin (Si, i are 1,…,4) as a main plot with different levels about 0, 1, 2,3, 4 ml L−1 of cytokinin solutions, respectively. The second factorswere the third generation of the polyploid chrysanthemum (G) genotype as a sub plot with 4 genotype, namely: g0 = KRA0, g1 = KRA1, g2 = KRA2, and g3 = KRA3. Based on the experiments and analysis results, it was found that the treatment of 3 ml L−1 of the cytokinin solution produced 16 shoots while the polyploid chrysanthemum genotype KRA2 gave the best response to the number of shoots at 12 WAP compared to other polyploid chrysanthemum genotypes.

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Respon Padi Gogo Terhadap Residu Pupuk Organik

Agrotek ◽

10.30862/agt.v3i2.566 ◽

2018 ◽

Vol 3 (2) ◽

Author(s):

Baso Daeng

Keyword(s):

Environmental Sustainability ◽

Upland Rice ◽

Organic Fertilizer ◽

Growing Season ◽

Chicken Manure ◽

Good Effect ◽

Best Response ◽

Different Types ◽

Main Plot ◽

Plot Design

The rate of conversion of paddy fields and irrigation water crisis suggest to consider the development of upland rice.� Empowerment of organic-based dryland done to increase rice, as well as environmental sustainability efforts.� The purpose of this experiment was to determine the effect of organic fertilizer residue to upland rice in the second growing season.� Experiments using a split-split plot design.� The main plot consisted of a dosage of 50% and 100% organic fertilizer in the first growing season.� Sub plot consisted of chicken manure (20 tons ha-1), Centrosema� pubescens (4.3 tons ha-1) + chicken manure (10 tons ha-1), and Thitonia diversifolia (4.3 tons ha-1) + chicken manure (10 tons ha-1).� Sub-sub plot consist of Danau Gaung and Batu Tegi varieties.� The different types of fertilizer had no effect on plant productivity.� The addition of Thitonia diversifolia gave a good effect on some growth variable and its resistance due pathogen attack.� Batu Tegi varieties are varieties that give the best response from an organic fertilizer.� Interaction between dosage, type of fertilizer, and varieties do not provide areal impact.

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New Nucleotide Polymorphisms in the BTC1 gene of Sugar Beet

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-6-49-54 ◽

2020 ◽

Vol 36 (6) ◽

pp. 49-54

Author(s):

A.A. Nalbandyan ◽

T.P. Fedulova ◽

I.V. Cherepukhina ◽

T.I. Kryukova ◽

N.R. Mikheeva ◽

...

Keyword(s):

Sugar Beet ◽

Flowering Time ◽

Molecular Genetic ◽

Bioinformatic Analysis ◽

Early Flowering ◽

Nucleotide Polymorphisms ◽

Nucleotide Substitutions ◽

Time Control ◽

Flowering Time Control ◽

Exon 10

The flowering time control gene of various sugar beet plants has been studied. The BTC1 gene is a regulator for the suppressor (flowering time 1) and inducer (flowering time 2) genes of this physiological process. The F9/R9 primer pair was used for polymerase chain reaction; these primers are specific to the BTC1 gene region containing exon 9, as well as intron and exon 10. For the first time, nucleotide substitutions in exon 10 of BTC1 gene were identified in bolting sensitive samples (HF1 and BF1), which led to a change in the amino acid composition of the coded polypeptide chain. Based on the results of bioinformatic analysis, it can be assumed that certain nucleotide polymorphisms in the BTC1 gene may determine with a high probability the predisposition of sugar beet genotypes to early flowering. The use of the Geneious Prime tool for the analysis of the BTC1 gene sequences may allow the culling of genotypes prone to early flowering at early stages of selection. sugar beet, flowering gene, BTC1, genetic polymorphism, PCR, molecular genetic markers, selection

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Quantitative Control of Early Flowering in White Lupin (Lupinus albus L.)

International Journal of Molecular Sciences ◽

10.3390/ijms22083856 ◽

2021 ◽

Vol 22 (8) ◽

pp. 3856

Author(s):

Sandra Rychel-Bielska ◽

Anna Surma ◽

Wojciech Bielski ◽

Bartosz Kozak ◽

Renata Galek ◽

...

Keyword(s):

Flowering Time ◽

Association Studies ◽

Lupinus Albus ◽

Genotypic Diversity ◽

Early Flowering ◽

White Lupin ◽

Genome Wide Association Studies ◽

Interacting Protein ◽

Major Qtls ◽

Lupinus Albus L

White lupin (Lupinus albus L.) is a pulse annual plant cultivated from the tropics to temperate regions for its high-protein grain as well as a cover crop or green manure. Wild populations are typically late flowering and have high vernalization requirements. Nevertheless, some early flowering and thermoneutral accessions were found in the Mediterranean basin. Recently, quantitative trait loci (QTLs) explaining flowering time variance were identified in bi-parental population mapping, however, phenotypic and genotypic diversity in the world collection has not been addressed yet. In this study, a diverse set of white lupin accessions (n = 160) was phenotyped for time to flowering in a controlled environment and genotyped with PCR-based markers (n = 50) tagging major QTLs and selected homologs of photoperiod and vernalization pathway genes. This survey highlighted quantitative control of flowering time in white lupin, providing statistically significant associations for all major QTLs and numerous regulatory genes, including white lupin homologs of CONSTANS, FLOWERING LOCUS T, FY, MOTHER OF FT AND TFL1, PHYTOCHROME INTERACTING FACTOR 4, SKI-INTERACTING PROTEIN 1, and VERNALIZATION INDEPENDENCE 3. This revealed the complexity of flowering control in white lupin, dispersed among numerous loci localized on several chromosomes, provided economic justification for future genome-wide association studies or genomic selection rather than relying on simple marker-assisted selection.

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Identification and characterization of regulatory pathways involved in early flowering in the new leaves of alfalfa (Medicago sativa L.) by transcriptome analysis

BMC Plant Biology ◽

10.1186/s12870-020-02775-9 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Dongmei Ma ◽

Bei Liu ◽

Lingqiao Ge ◽

Yinyin Weng ◽

Xiaohui Cao ◽

...

Keyword(s):

Medicago Sativa ◽

Secondary Metabolism ◽

Flowering Time ◽

Degradation Pathway ◽

Early Flowering ◽

Receptor Kinase ◽

Leucine Rich Repeat ◽

Kinase Family ◽

Pathogenesis Related ◽

Early Flowering Phenotype

Abstract Background Alfalfa (Medicago sativa L.) is a perennial legume extensively planted throughout the world as a high nutritive value livestock forage. Flowering time is an important agronomic trait that contributes to the production of alfalfa hay and seeds. However, the underlying molecular mechanisms of flowering time regulation in alfalfa are not well understood. Results In this study, an early-flowering alfalfa genotype 80 and a late-flowering alfalfa genotype 195 were characterized for the flowering phenotype. Our analysis revealed that the lower jasmonate (JA) content in new leaves and the downregulation of JA biosynthetic genes (i.e. lipoxygenase, the 12-oxophytodienoate reductase-like protein, and salicylic acid carboxyl methyltransferase) may play essential roles in the early-flowering phenotype of genotype 80. Further research indicated that genes encode pathogenesis-related proteins [e.g. leucine rich repeat (LRR) family proteins, receptor-like proteins, and toll-interleukin-like receptor (TIR)-nucleotide-binding site (NBS)-LRR class proteins] and members of the signaling receptor kinase family [LRR proteins, kinases domain of unknown function 26 (DUF26) and wheat leucine-rich repeat receptor-like kinase10 (LRK10)-like kinases] are related to early flowering in alfalfa. Additionally, those involved in secondary metabolism (2-oxoglutarate/Fe (II)-dependent dioxygenases and UDP-glycosyltransferase) and the proteasome degradation pathway [really interesting new gene (RING)/U-box superfamily proteins and F-box family proteins] are also related to early flowering in alfalfa. Conclusions Integrated phenotypical, physiological, and transcriptomic analyses demonstrate that hormone biosynthesis and signaling pathways, pathogenesis-related genes, signaling receptor kinase family genes, secondary metabolism genes, and proteasome degradation pathway genes are responsible for the early flowering phenotype in alfalfa. This will provide new insights into future studies of flowering time in alfalfa and inform genetic improvement strategies for optimizing this important trait.

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Effect of varieties and weeding regimes on weed and performance of

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.1(3).p121-130 ◽

2011 ◽

Vol 1 (3) ◽

pp. 121-130

Author(s):

U. Ismaila ◽

M. G. Kolo ◽

A. U. Gbanguba

Keyword(s):

Oryza Sativa ◽

Active Ingredient ◽

Oryza Sativa L ◽

Weed Suppression ◽

Dichlorophenoxyacetic Acid ◽

Aerobic Rice ◽

Split Plot ◽

Main Plot ◽

And Performance ◽

Plot Design

A Field trial was conducted at the aerobic rice experimental field of the NationalCereals Research Institute (NCRI), Badeggi, Nigeria (9 ° 04 N and 6° 7 E)in 2007 and 2008 wet seasons to determine the weed competitiveness andperformance of inter Ã¢â‚¬Â and intraÃ¢â‚¬Âspecific aerobic rice (Oryza sativa L.) varietiesunder seven weeding regimes. The trial was laid out in a split plot designwith the two varieties of rice (NERICA 1 as inter Ã¢â‚¬Âspecific and FARO 46 asintra Ã¢â‚¬Âspecific) assigned to the main plot while seven weeding regimes (hoeweeding at 25, 45, and 65 days after sowing (DAS), 25 DAS, 45 DAS, 25 and45 DAS, application of 3’,3’ Ã¢â‚¬Â dichloropropionanilide /2, 4 – Dichlorophenoxyacetic acid (orizo plus R) by Candel Company Ltd. at 3.5 kg active ingredient(ai )ha Ã¢â‚¬Â1 at 25 DAS, hoe weeding at 25 DAS followed by orizo plus at 3.5 kg aiat 45 DAS and weedy check) constituted the sub – plots. The treatmentswere replicated three times in a split plot design. From the results, interspecificvariety (NERICA1) had better weed suppression ability with highergrain yield of 3.1 t ha Ã¢â‚¬Â1 than the intraÃ¢â‚¬Âspecific variety (FARO 46). Weed controlwas better when hoe weeding was done at 25, 45 and 65 DAS or 25 and45 DAS and at 25 DAS followed by orizo plus at 45 DAS than in other treatments.However, hoe weeding at 25, 45 and 65 DAS gave significantlygreater grain yield than other treatments. Although economic yield was obtainedfrom hoe weeding at 25 DAS followed by orizo plus at 45 DAS.

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Allelopathy and Allelochemicals of Eragrostis plana (Poaceae) and its Relation with Phenology and Nitrogen Fertilization

Planta Daninha ◽

10.1590/s0100-83582017350100028 ◽

2017 ◽

Vol 35 (0) ◽

Cited By ~ 3

Author(s):

K. CECCHIN ◽

A. FAVARETTO ◽

S.M. SCHEFFER-BASSO ◽

C.D. BERTOL ◽

S.O. CHINI

Keyword(s):

Nitrogen Fertilization ◽

High Performance ◽

Phenological Stage ◽

Flowering Stage ◽

Chromatography Analysis ◽

Allelopathic Activity ◽

Aerial Parts ◽

Main Plot ◽

Chemical Attributes ◽

Plot Design

ABSTRACT This study was conducted in order to verify if the phenological stage and the nitrogen fertilization interfere in the allelopathic activity and in the concentration of potentially allelopathic phenolic compounds of tough lovegrass (Eragrostis plana). The assay consisted of a bifactor 3 (0.100 and 200 kg N ha-1) x 2 (harvested in vegetative and reproductive stages), in a split plot design. The N doses constituted the main plot and the phenological stage during the harvest the subplots, resulting in six treatments. The tough lovegrass plants derived from each of the treatments were subjected to allelopathy bioassays, in which aqueous extracts of the aerial parts were applied to lettuce cypselae (Lactuca sativa) and to phytochemicals tests when ethanolic extracts were used, with subsequent partition with ethyl acetate, followed by a high-performance liquid chromatography analysis. There was nitrogen x phenological stage interaction on biological and chemical attributes. The allelopathic extracts were, in descending order of inhibition of germination, those from plants harvested at the vegetative stage and fertilized with 100 kg N and at the flowering stage with 200 kg N, which showed the highest catechin concentrations. The caffeic, ferulic, p-coumaric and vanillic acids were in a higher concentration in flowered and fertilized plants with 0 or 200 kg N. The management of the nitrogen fertilization and the harvesting age influence the allelopathic activity and the phytochemical composition of tough lovegrass.

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