Antifungal, Anti-aflatoxigenic, Antioxidant Activity and in vivo Efficacy of Essential Oil of the Aerial Parts of Thymus capitatus (L.) Hoffmanns & Link

Aspergillus flavus has been reported to be the most common fungus used as food by human beings. This fungus may have the potential to produce aflatoxin B1 (AFB1), which is reported as being the most hepatotoxic, teratogenic, mutagenic, and immunosuppressive to humans and other livestock. Therefore, the objective of this work was to study the chemical profile of Thymus capitatus essential oil (EO) and the inhibition of growth of Aspergillus flavus E73 and AFB1 production by the EO. The antioxidant activity and phytotoxicity were also evaluated. The gas chromatography– mass spectrometry (GC–MS) analysis showed that the major components of Thymus capitatus EO were thymol (25.82%), linalool (23.40%), geraniol (14.22%), pcymen- 3-ol (8.93%), and p-cymene (6.76%). The results showed that the EO could inhibit the growth of Aspergillus flavus E73 in the range of 40.72 to 87.00%. The minimal inhibitory concentration (MIC) of Thymus capitatus EO against Aspergillus flavus E73 was found to be at 1.00 mg/ml. The oil revealed complete inhibition of dry mycelium weight and AFB1 production at 1.00 mg/ml. The EO revealed a broad spectrum of fungitoxicity against some fungi. The antioxidant activity was also assessed where IC50 (when initial concentration is reduced to half) and β- carotene/linoleic acid inhibition percentage of Thymus capitatus EO were 619.16 ± 3.94 μg/ml and 65.55%, respectively, while the total phenolic content was 21.45 μg/mg. The EO showed non-phytotoxicity on two varieties of wheat seeds. These findings demonstrated that EO could be good alternative to protect food.

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Volatile Fraction Composition and Total Phenolic and Flavonoid Contents of Elionurus hensii—Antioxidant Activities of Essential Oils and Solvent Extracts

Natural Product Communications ◽

10.1177/1934578x1300800528 ◽

2013 ◽

Vol 8 (5) ◽

pp. 1934578X1300800 ◽

Cited By ~ 4

Author(s):

Yin Yang ◽

Marie-Cécile De Cian ◽

Samuel Nsikabaka ◽

Pierre Tomi ◽

Thomas Silou ◽

...

Keyword(s):

Gas Chromatography ◽

Antioxidant Activity ◽

Essential Oil ◽

Ethyl Acetate ◽

Gas Chromatography Mass Spectrometry ◽

Total Phenolic ◽

Volatile Fraction ◽

Aerial Parts ◽

Reducing Ability ◽

Phenolic And Flavonoid

The objective of this study was to characterize Elionurus hensii in terms of the chemical composition of its essential oil and volatile fractions, the total phenolic and flavonoid contents of solvent extracts and antioxidative activity. Analysis of E. hensii essential oil samples from Congo was carried out using Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (MS). The essential oil of the aerial parts consisted predominantly of oxygenated monoterpene compounds, of which the main components were menthane alcohol stereoisomers, whereas the essential oil of the root was characterized by a high content of oxygenated sesquiterpenes, the main component of which was aristolone. The amounts of total phenolics and flavonoids in the solvent extracts (dichloromethane, ethyl acetate and methanol) were determined spectrometrically. The essential oil did not exhibit significant antioxidant activity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS•+) scavenging ability, and exhibited only slight Fe3+ reducing ability. In contrast, the antioxidant activity of the solvent extracts of the aerial parts increased in the order ethyl acetate > methanol > dichloromethane, and that of the solvent extracts of the root increased in the order methanol > ethyl acetate > dichloromethane. Finally, a relationship was observed between the antioxidant activity and the total phenolic and flavonoid levels of the extract.

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Phytotoxicity of Essential Oils on Selected Weeds: Potential Hazard on Food Crops

Plants ◽

10.3390/plants7040079 ◽

2018 ◽

Vol 7 (4) ◽

pp. 79 ◽

Cited By ~ 12

Author(s):

María Ibáñez ◽

María Blázquez

Keyword(s):

Seed Germination ◽

Essential Oil ◽

Essential Oils ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Food Crops ◽

Potential Hazard ◽

Spectrometry Analysis

The chemical composition of winter savory, peppermint, and anise essential oils, and in vitro and in vivo phytotoxic activity against weeds (Portulaca oleracea, Lolium multiflorum, and Echinochloa crus-galli) and food crops (maize, rice, and tomato), have been studied. Sixty-four compounds accounting for between 97.67–99.66% of the total essential oils were identified by Gas Chromatography-Mass Spectrometry analysis. Winter savory with carvacrol (43.34%) and thymol (23.20%) as the main compounds produced a total inhibitory effect against the seed germination of tested weed. Menthol (48.23%), menthone (23.33%), and iso-menthone (16.33%) from peppermint only showed total seed germination inhibition on L. multiflorum, whereas no significant effects were observed with trans-anethole (99.46%) from anise at all concentrations (0.125–1 µL/mL). Low doses of peppermint essential oil could be used as a sustainable alternative to synthetic agrochemicals to control L. multiflorum. The results corroborate that in vivo assays with a commercial emulsifiable concentrate need higher doses of the essential oils to reproduce previous in vitro trials. The higher in vivo phytotoxicity of winter savory essential oil constitutes an eco-friendly and less pernicious alternative to weed control. It is possible to achieve a greater in vivo phytotoxicity if less active essential oil like peppermint is included with other active excipients.

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Antioxidant Activities and Reduced Amyloid-β Toxicity of 7-Hydroxycalamenene Isolated from the Essential Oil of Zelkova serrata Heartwood

Natural Product Communications ◽

10.1177/1934578x1601100943 ◽

2016 ◽

Vol 11 (9) ◽

pp. 1934578X1601100

Author(s):

Pei-Ling Yen ◽

Sen-Sung Cheng ◽

Chia-Cheng Wei ◽

Huan-You Lin ◽

Vivian Hsiu-Chuan Liao ◽

...

Keyword(s):

Essential Oil ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Amyloid Β ◽

Total Phenolic ◽

C Elegans ◽

In Vivo Assays ◽

Zelkova Serrata

The in vitro and in vivo antioxidant activities and its potential to protect against amyloid-β toxicity of essential oils from Zelkova serrata (Thunb.) Makino were investigated in the model organism Caenorhabditis elegans. The results revealed that the essential oil of Z. serrata heartwood exhibited great radical scavenging activities and high total phenolic content. In vivo assays showed significant inhibition of oxidative damage in wild-type C. elegans under juglone-induced oxidative stress and heat shock. Based on results from both in vitro and in vivo assays, the major compound in essential oil of heartwood, (-)-(1 S, 4 S)-7-hydroxycalamenene (1 S, 4 S-7HC), may contribute significantly to the observed antioxidant activity. Further evidence showed that 1 S, 4 S-7HC significantly delayed the paralysis phenotype in amyloid beta-expressing transgenic C. elegans. These findings suggest that 1 S, 4 S-7HC from the essential oil of Z. serrata heartwood has potential as a source for antioxidant or Alzheimer's disease treatment.

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Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201520150056 ◽

2015 ◽

Vol 87 (2 suppl) ◽

pp. 1397-1408 ◽

Cited By ~ 29

Author(s):

GABRIELA L. DA SILVA ◽

CAROLINA LUFT ◽

ADROALDO LUNARDELLI ◽

ROBSON H. AMARAL ◽

DENIZAR A. DA SILVA MELO ◽

...

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Therapeutic Potential ◽

Positive Control ◽

Croton Oil ◽

Antinociceptive Effects ◽

Anti Inflammatory ◽

Pre Treatment ◽

Dose Dependent

Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The inflammatory response evoked by carrageenan and by croton oil was reduced through the pre-treatment of animals with LEO. In the pleurisy model, the drug used as positive control, dexamethasone, was more efficacious. However, in the ear swelling, the antiedematogenic effect of the oil was similar to that observed for dexamethasone. In the formalin test, LEO consistently inhibited spontaneous nociception and presented a similar effect to that of tramadol. The results of this study reveal (in vivo) the analgesic and anti-inflammatory activities of LEO and demonstrates its important therapeutic potential.

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Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽

10.3390/molecules23092216 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2216 ◽

Cited By ~ 7

Author(s):

Cai-Ning Zhao ◽

Guo-Yi Tang ◽

Qing Liu ◽

Xiao-Yu Xu ◽

Shi-Yu Cao ◽

...

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

High Performance ◽

Antioxidant Properties ◽

Dry Weight ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Herbal Tea ◽

Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

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GC-MS analysis and biological activities of Thymus vulgaris and Mentha arvensis essential oil

Turkish Journal of Biochemistry ◽

10.1515/tjb-2018-0258 ◽

2019 ◽

Vol 44 (3) ◽

pp. 388-396

Author(s):

Ashfaq Ahmad Khan ◽

Muhammad Shoaib Amjad ◽

Saboon

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Biological Activities ◽

Colorimetric Method ◽

Diffusion Method ◽

Total Phenolic ◽

Thymus Vulgaris ◽

Dpph Assay ◽

Mentha Arvensis ◽

Ms Analysis

Abstract Background Essential oils are chemical products produced by odoriferous glands from a variety of plants. These essential oil have many health benefits i.e. antiseptic, anti-inflammatory and antimicrobial activities. So due to these medicinal properties present study was designed to analyze essential oil of Thymus vulgaris and Mentha arvensis for their chemical composition and biological activities. Materials and methods Essential oil from these plants were extracted by hydrodistillation method, and analyzed by GC-MS. To test the microbial activity of these oil disk diffusion method and micro wells method were used. For free radical scavenging DPPH assay was used. However total phenolic content was measured by colorimetric method. Results The GC-MS analysis of T. vulgaris oil showed the presence of 47 chemical compounds among which thymol, terpinene, p-cymene and carvacrol were major. However essential oil of M. arvensis showed the presence of 28 constituents, among which the Menthone, Menthol, Isomenthone, Eucalyptol, neo-Menthol, cis-Piperitone oxide, Linalool, Thymol, Limonene, and α-Phellandrene were major. Essential oil from both these plant tested for antimicrobial activity showed that the T. vulgaris oil was effective against seven bacterial strains and the essential oil of M. arvensis was effective against six bacterial strain. The antioxidant activity of both samples by DPPH assay which showed positive result. Conclusion As both species showed the presence of active components, positive microbial activities, and antioxidant activity so, research should be carried on for further biological activities of these oil for betterment of living beings.

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Staphylococcus saprophyticus L-38 produces volatile 3,3-dimethyl-1,2-epoxybutane with strong inhibitory activity against Aspergillus flavus germination and aflatoxin production

World Mycotoxin Journal ◽

10.3920/wmj2019.2495 ◽

2020 ◽

Vol 13 (2) ◽

pp. 247-258 ◽

Cited By ~ 1

Author(s):

A.D. Gong ◽

G.J. Sun ◽

Z.Y. Zhao ◽

Y.C. Liao ◽

J.B. Zhang

Keyword(s):

Food Safety ◽

Antifungal Activity ◽

Aspergillus Flavus ◽

Plant Pathogens ◽

Aflatoxin Production ◽

Gas Chromatography Mass Spectrometry ◽

Total Peak Area ◽

Staphylococcus Saprophyticus

Controlling proliferation and aflatoxin production by Aspergillus flavus is a pressing challenge for global food safety and security. Marine bacterium Staphylococcus saprophyticus strain L-38 showed excellent antifungal activity toward A. flavus in vitro and in vivo. In sealed, non-contact confrontation assays, L-38 completely inhibited conidial germination and mycelial growth of A. flavus through the production of volatile organic compounds (VOCs). Gas chromatography-mass spectrometry identified 3,3-dimethyl-1,2-epoxybutane (3-DE) as the most abundant VOC (32.61% of total peak area, 78% matching). Exposure of A. flavus cultures to synthetic 3-DE similarly demonstrated strong inhibition of growth. Moreover, culture of L-38 in a sealed chamber with maize or peanuts artificially inoculated with A. flavus, at high water activity, resulted in significant inhibition of A. flavus germination and aflatoxin biosynthesis. Scanning electron microscopy of these samples revealed severe damage to conidial cells and hyphae compared to samples not exposed to L-38. L-38 also showed broad and effective antifungal activity toward eight other phytopathogenic fungi including Aspergillus niger, Fusarium verticillioides, Fusarium graminearum, Sclerotinia sclerotiorum, Rhizoctonia solani, Alternaria alternata, Monilinia fructicola, and Botrytis cinerea. This work introduces S. saprophyticus L-38 as a potential biocontrol agent and demonstrates the efficacy of the volatile 3-DE in the control of A. flavus and other destructive plant pathogens for post-harvest food safety.

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ANTIOXIDANT ACTIVITY, PHYTOCHEMICAL ANALYSIS AND TOTAL POLYPHENOLICS CONTENT OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS FROM COMMELINA NUDIFLORA

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i8.25922 ◽

2018 ◽

Vol 10 (8) ◽

pp. 36 ◽

Cited By ~ 4

Author(s):

Muhammad Dawood Shah ◽

Mohammad Iqbal

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Ethyl Acetate ◽

Palmitic Acid ◽

Methanol Extract ◽

Radical Scavenging ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Hexadecanoic Acid ◽

Methanol Extracts

Objective: In the present study, the essential oil, methanol extract, and methanol fractions (n-hexane, chloroform, ethyl acetate, and n-butanol) obtained from Commelina nudiflora were investigated for the free radical scavenging effects and phytochemical analysis.Methods: The antioxidative effect of the essential oil, methanol extracts and methanol fractions were evaluated using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Total phenolic and ﬂavonoid contents were determined using Folin-Ciocalteau and aluminium chloride reagents respectively. The phytochemical analyses of the essential oil, methanol extracts and methanol fractions were performed by gas chromatography and mass spectrometry (GCMS). Results: The antioxidant, total phenolic and total flavonoid contents of butanol, ethyl acetate and chloroform fractions were higher followed by methanol extract, hexane fraction and essential oil. Phytochemical analysis indicated the presence of alkaloid, saponin, steroid, phytosterols, triterpenoids and tannins etc. The identified bioactive constituents of essential oil, methanol extract and methanol fractions of C. nudiflora were indole, 2-methoxy-4-vinylphenol, 2-pentadecanone, 6,10,14-trimethyl, phenol, benzyl alcohol, eugenol, phenol, 2, 4-bis (1,1-dimethylethyl), hexadecanoic acid, ethyl ester (palmitic acid ester), n-hexadecanoic acid (palmitic acid), 9, 12-octadecadienoic acid, (linoleic acid) and phytol. All identified bioactive compounds and their derivatives were generally reported with antimicrobial, antioxidant, anti-inflammatory and antitumor properties.Conclusion: The obtained data suggest that the essential oil, methanol extract and methanol fractions of C. nudiflora possess remarkable antioxidant activities and vital phytochemicals. Thus the plant can be a utilized as a potential source of nutraceutical with antioxidant activity.

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Development of a Millet Starch Edible Film Containing Clove Essential Oil

Foods ◽

10.3390/foods9020184 ◽

2020 ◽

Vol 9 (2) ◽

pp. 184 ◽

Cited By ~ 1

Author(s):

Alaa G. Al-Hashimi ◽

Altemimi B. Ammar ◽

Lakshmanan G. ◽

Francesco Cacciola ◽

Naoufal Lakhssassi

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Antioxidant Properties ◽

Control Sample ◽

Barrier Properties ◽

Edible Film ◽

Gas Chromatography Mass Spectrometry ◽

Syzygium Aromaticum ◽

Preservation Method ◽

Clove Essential Oil

Medicinal plants contain various secondary metabolites. The present study analyzed the essential oil of buds from clove (Syzygium aromaticum L.; Family: Myrtaceae) using gas chromatography-mass spectrometry (GC-MS). GC-MS analysis showed the presence of six major phytoconstituents, such as eugenol (66.01%), caryophyllene (19.88%), caryophyllene oxide (5.80%), phenol, 2-methoxy-4-(2-propenyl)-acetate (4.55%), and humulene (3.75%). The effect of clove essential oils (CEO) at 0%, 1%, 2%, and 3% (w/w) on the mechanical and barrier properties of starch films was evaluated. The tensile strength (TS) and elongation (E) of films with clove essential oil were 6.25 ± 0.03 MPa and 5.67% ± 0.08%, respectively. The antioxidant activity of the films significantly increased the millet starch film and presented the lowest antioxidant activity (0.3%) at a 30 minute incubation for the control sample, while increasing CEO fraction in the starch film lead to an increase in antioxidant activity, and the 3% CEO combined film presented the highest antioxidant activity (15.96%) at 90 min incubation. This finding could be explained by the incorporation of clove oil containing antioxidant properties that significantly increased with the incorporation of CEO (p < 0.05). A zone of inhibition ranging from 16 to 27 mm in diameter was obtained when using a concentration of CEO ranging from 1% to 3%. We also observed the presence of an antimicrobial activity on several tested microorganism including Escherichia coli, Pseudomonas aeruginosa, Enterobacter sp, Bacillus cereus, Staphylococcus aureus, and Trichoderma fungi. Thus, the current study reveals the possibility of using a millet starch edible film as a preservation method.

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Major Antifungals in Nutmeg Essential Oil against Aspergillus flavus and A. ochraceus

Journal of Food Research ◽

10.5539/jfr.v4n1p51 ◽

2014 ◽

Vol 4 (1) ◽

pp. 51 ◽

Cited By ~ 7

Author(s):

Vania Maria Moreira Valente ◽

Gulab Newandram Jham ◽

Carolina Marangon Jardim ◽

Onkar Dev Dhingra ◽

Ion Ghiviriga

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Aspergillus Flavus ◽

High Performance ◽

Gas Chromatography Mass Spectrometry ◽

Ionization Detector ◽

Flame Ionization ◽

Storage Fungi ◽

Pure Compounds ◽

Nutmeg Essential Oil

Aiming to substitute toxic synthetic fungicides, the activity of nutmeg (Myristica fragrans) essential oil (EO, obtained by hydrodistillation) was investigated against two important storage fungi-Aspergillus flavus A. ochraceus. The activity of crude nutmeg EO was investigated using poison food assay (PFA). At a concentration of 0.1%, the EO inhibited A. flavus and A. ochraceus growth by 43 and 65%, respectively. At a concentration of 0.3 %, A. flavus and A. ochraceus inhibitions were 84 and 79%, respectively. The crude nutmeg EO on fractionation by preparative TLC-bioautography presented one band from which two pure compounds were isolated by semi-preparative normal-phase high performance liquid chromatography. Myristicin and safrole were identified by nuclear magnetic resonance (1H and 13C) and gas chromatography-mass spectrometry. The relative % of myristicin and safrol in the crude EO was 10.8 and 2.9, respectively, determined by gas chromatography with a flame ionization detector. The crude EO, the isolated active fraction, isolated myristicin and standard myristicin presented similar activities against the two fungi at concentrations of 0.1 and 0.3% by PFA. Based on these results it is concluded that myristicin is the major antifungal in nutmeg EO against A. flavus and A. ochraceus.

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