scholarly journals The Hepatoprotective Effects of Basil Leaf (Ocimum sanctum L.) Extract on Paracetamol Induced Liver Damage in Male Rat

2021 ◽  
Vol 7 (2) ◽  
pp. 444-450
Author(s):  
Ronaldo Panggabean ◽  
Nofita ◽  
Ade Maria Ulfa
Keyword(s):  
Liver Damage ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Male Rat ◽  
Ocimum Sanctum ◽  
Sprague Dawley ◽  
Percolation Method ◽  
Sprague Dawley Rats

Basil leaf have antioxidants such as flavonoids, so it is thought to have a hepatoprotective effect. This study aims to investigate the effect of basil leaf extract on SGOT and SGPT levels in male rats induced by paracetamol. Basil leaf extract was carried out by the percolation method using ethyl acetate solvent, Some 20 male sprague dawley rats were randomly divided into 5 groups. Basil leaf extract (400 mg/kgBB and 600 mg/kgBB) and sylimarin (100 mg/kgBB) were carried out every day for 28 days, paracetamol was induced 24 hours after giving the last day of basil leaf extract. The parameters measured were SGOT and SGPT level to assess the effect of basil leaf extract on liver damage caused by paracetamol. The results showed that basil leaf extract (400 mg/kgBB dan 600 mg/kgBB) showed that the activities of SGOT and SGPT levels were statistically significant (p<0,05) to negative control. Basil leaf extract shows the effect of hepatoprotector on liver induced by paracetamol, however the effect given was not able to equate with positive control.

scholarly journals Red Rosella (Hibiscus sabdariffa Linn.) Petal Brew is Able to Reduce the Sprague Dawley MDA Rate in Rats Exposed to Waste Cooking Oil

2018 ◽  
Vol 54 (3) ◽  
pp. 167
Author(s):  
Arya Ulilalbab ◽  
Eni Maskanah
Keyword(s):  
Oxidative Stress ◽  
Waste Cooking Oil ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
P Value ◽  
Sprague Dawley ◽  
Cooking Oil ◽  
Randomized Design ◽  
Sprague Dawley Rats

Food and snacks sold are usually fried using oil that has been used for frying repeatedly. Oil that is repeatedly used for frying is often called waste cooking oil. Waste cooking oil is a source of exogenous free radicals that can trigger oxidative stress. To prevent this, sufficient antioxidant intake is needed. One source of antioxidants is red rosella. The purpose of this study was to analyze the effect of giving red rosella petals on the conditions of oxidative stress in Sprague dawley rats exposed to waste cooking oil through MDA testing. The research method used was Completely Randomized Design (CRD). The sample consisted of 24 male rats which were randomly selected and divided into 4 groups: negative control (no treatment), positive control (administered with waste cooking oil of 8.92 meq/kg as much as 2 ml/kgBW), treatment 1 (administered with waste cooking oil of 8.92 meq/kg as much as 2 ml/kgBW and red rosella petal brew dosed of 540 mg/kgBW, and treatment 2 (administered with waste cooking oil of 8.92 meq/kg as much as 2 ml/kgBW and red rosella petals brew dosed of 810 mg/kgBW). The results of the one way ANOVA analysis (a=1%) and the Tukey HSD test showed the p value of MDA=0.00, indicating that all treatments had significant effect. In further tests, it was found that all treatments contained differences in MDA values. The best value in the treatment was by giving a dose of 810 mg/kgBW (serum MDA of 2.22 nmol/ml). It can be concluded that the administration of red rosella petal in doses of 540 mg/kgBW (EC50=407.52 ppm) and 810 mg/kgBW (EC50=247.82 ppm) can improve the oxidative stress of Sprague dawley rats.Food and snacks sold are usually fried using oil that has been used for frying repeatedly. Oil that is repeatedly used for frying is often called waste cooking oil. Waste cooking oil is a source of exogenous free radicals that can trigger oxidative stress. To prevent this, sufficient antioxidant intake is needed. One source of antioxidants is red rosella. The purpose of this study was to analyze the effect of giving red rosella petals on the conditions of oxidative stress in Sprague dawley rats exposed to waste cooking oil through MDA testing. The research method used was Completely Randomized Design (CRD). The sample consisted of 24 male rats which were randomly selected and divided into 4 groups: negative control (no treatment), positive control (administered with waste cooking oil of 8.92 meq/kg as much as 2 ml/kgBW), treatment 1 (administered with waste cooking oil of 8.92 meq/kg as much as 2 ml/kgBW and red rosella petal brew dosed of 540 mg/kgBW, and treatment 2 (administered with waste cooking oil of 8.92 meq/kg as much as 2 ml/kgBW and red rosella petals brew dosed of 810 mg/kgBW). The results of the one way ANOVA analysis (a=1%) and the Tukey HSD test showed the p value of MDA=0.00, indicating that all treatments had significant effect. In further tests, it was found that all treatments contained differences in MDA values. The best value in the treatment was by giving a dose of 810 mg/kgBW (serum MDA of 2.22 nmol/ml). It can be concluded that the administration of red rosella petal in doses of 540 mg/kgBW (EC50=407.52 ppm) and 810 mg/kgBW (EC50=247.82 ppm) can improve the oxidative stress of Sprague dawley rats.

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

scholarly journals PENGARUH SINBIOTIK KEFIR PISANG BATU TERHADAP KADAR KOLESTEROL-LDL DAN KOLESTEROL-HDL TIKUS MODEL SINDROM METABOLIK

2020 ◽  
Vol 7 (2) ◽  
pp. 280-288
Author(s):  
Dina Khoiriyah ◽  
Taufik Maryusman ◽  
Santi Herlina
Keyword(s):  
Risk Factors ◽  
Metabolic Syndrome ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Musa Balbisiana ◽  
Cholesterol Levels ◽  
Sprague Dawley Rats

Effect of Banana Kefir Synbiotic on LDL-Cholesterol and HDL-Cholesterol of Metabolic Syndrome Rats Metabolic syndrome (SM) is characterized by several risk factors including dyslipidemia. This study aims to analyze the effect of kefir synbiotic produced from banana stone flour (Musa balbisiana) on LDL-cholesterol and HDL-cholesterol of metabolic syndrome rat model. The 24 Sprague Dawley rats were divided into four groups, namely negative control (healthy rats fed standard feed), positive control (metabolic syndrome rats fed standard feed), treatment I and treatment II (metabolic syndrome rats each given synbiotic kefir banana stone flour 1.8 mL/200 g mouse BW/day and 3.6 mL/200 g mouse BW/day, respectively). The intervention was carried out for three weeks. After the intervention, the levels of LDL-cholesterol in treatment I and II experienced a decrease of 44.66% and 56.94%, respectively, while the-HDL-cholesterol levels in treatment I and II experienced an increase of 104.5% and 172.71%, respectively. The biggest change occurred in treatment II. Synbiotic kefir banana stone flour improved lipid profile in metabolic syndrome rats. Sindrom metabolik (SM) ditandai dengan beberapa faktor risiko termasuk dislipidemia. Penelitian ini bertujuan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu (Musa balbisiana) terhadap kadar kolesterol-LDL dan kolesterol-HDL tikus model SM. Subjek menggunakan 24 ekor tikus Sprague Dawley yang dibagi menjadi empat kelompok, yaitu kontrol negatif (tikus sehat yang diberi pakan standar), kontrol positif (tikus model SM yang diberi pakan standar), perlakuan I dan perlakuan II (tikus model SM yang masing-masing diberi sinbiotik kefir tepung pisang batu 1,8 mL/200 g BB tikus/hari dan 3,6 mL/200 g BB tikus/hari). Proses intervensi dilakukan selama tiga minggu. Setelah intervensi, kadar kolesterol-LDL perlakuan I dan II mengalami penurunan sebesar 44,66% dan 56,94%, sedangkan kadar kolesterol-HDL perlakuan I dan II mengalami peningkatan sebesar 104,5% dan 172,71%. Perubahan terbesar terjadi pada perlakuan II. Sinbiotik kefir tepung pisang batu memperbaiki profil lipid tikus sindrom metabolik.

scholarly journals THE EFFECT OF SECANG EXTRACT (CAESALPINIA SAPPAN LINN) ON THE WEIGHT AND HISTOLOGY APPEARANCE OF WHITE MALE RATS’ HEARTS INDUCED BY ISOPROTERENOL

2017 ◽  
Vol 9 ◽  
pp. 59
Author(s):  
Kristiana Nugraheni ◽  
Fadlina Chany Saputri
Keyword(s):  
Myocardial Infarction ◽  
Body Weight ◽  
White Male ◽  
Negative Control ◽  
Male Rats ◽  
Heart Cells ◽  
Sprague Dawley ◽  
Macroscopic Appearance ◽  
Caesalpinia Sappan ◽  
Sprague Dawley Rats

Objective: This study was conducted to determine the cardioprotective effect of secang extract on the heart cells of rats who suffered from myocardialinfarction induced by isoproterenol.Materials and Methods: Sprague Dawley rats were divided into six groups: Normal control, negative control, control extract (200 mg/kg), and threedifferent dose extract groups (50, 100, and 200 mg/kg body weight) that were given treatment for 30 days, and then, induced with isoproterenol.Observations were made for changes in the macroscopic appearance, cardiac weight, and histology of the cardiac organ.Results: The results showed a decrease in the incidence of myocardial infarction in rats given secang extract. The infarction area decreased withincreasing doses of extract. The weight of the heart in the control extract group was smaller than in the negative control group.Conclusions: Damage to heart cells, seen in the microscope, decreased with increasing doses.

Buprenorphine alleviation of pain does not compromise the rat monoarthritic pain model

2017 ◽  
Vol 16 (1) ◽  
pp. 167-167
Author(s):  
M.S. Berke ◽  
Klas S.P. Abelson
Keyword(s):  
Rat Model ◽  
Joint Stiffness ◽  
Positive Control ◽  
Negative Control ◽  
Pain Tolerance ◽  
Control Group ◽  
Mechanical Stimuli ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
A Minor

Abstract Aims This study investigated the effects of buprenorphine treatment on pain and welfare parameters and model specific parameters in a rat model of monoarthritis to eliminate unnecessary pain from this model. Methods 32 male Sprague Dawley rats were divided into four groups: (1) A negative control without arthritis receiving no analgesia. (2) A positive monoarthritic control group receiving no analgesia, but subcutaneous saline injections twice a day. (3) A positive control with monoarthritis receiving subcutaneous carprofen once a day and saline once a day. (4) A group with monoarthritis receiving subcutaneous buprenorphine twice a day. Monoarthritis was induced with an injection of 0.02 ml Complete Freund’s Adjuvant intra-articularly in the left tibiotarsal joint. Treatment with analgesia was initiated at day 15 and the rats were euthanized at day 23. Results The induced monoarthritis elicited a pronounced acute inflammation. Several parameters such as bodyweight, mobility, stance, joint-stiffness and lameness scores were affected. A marked mechanical hyperalgesia in the tarsal area was observed by Electronic Von Frey testing, but no severe compromise of the animal welfare was seen at any time. Signs of chronic development began to appear from day 10 after the monoarthritic induction. No significant change in serum cytokines and faecal corticosterone measurements was found after administration of buprenorphine. A minor decrease in body weight was seen, and a higher pain tolerance to mechanical stimuli was observed, indicating pain alleviation. The histological examination confirmed monoarthritic development in all monoarthritic rats and revealed periarticular lesions suggesting diffusion of adjuvant from intra-articular injection site to the periphery. Conclusions The study demonstrated that buprenorphine has an analgesic effect in the adjuvant induced monoarthritic rat model, without obvious interference with the development of arthritis.

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

scholarly journals Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats

2020 ◽  
Vol 31 (1) ◽  
pp. 49
Author(s):  
Festi Artika Sari ◽  
Willy Sandhika ◽  
Tri Hartini Yuliawati
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Leaf Extract ◽  
Inflammatory Cell ◽  
Negative Control ◽  
Inflammatory Cell Infiltration ◽  
Male Rats ◽  
Cell Infiltration ◽  
Ocimum Sanctum ◽  
Control Group

<p class="ISIABSTRAKINGGRIS">Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value &lt;0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.</p>

scholarly journals ACUTE TOXICITY OF CHITOSAN NANOPARTICLES CONTAINING MAHKOTA DEWA (PHALERIA MACROCARPA) LEAF EXTRACT AND ANTI-INFLAMMATORY EFFECTS IN A DEXTRAN SODIUM SULFATE-INDUCED MOUSE MODEL OF ULCERATIVE COLITIS

2018 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Ari Estuningtyas ◽  
Santi Widiasari ◽  
Kusmardi Kusmardi
Keyword(s):  
Acute Toxicity ◽  
Leaf Extract ◽  
Chitosan Nanoparticles ◽  
Toxicity Testing ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Phaleria Macrocarpa ◽  
Anti Inflammatory

Objective: The plant mahkota dewa (Phaleria macrocarpa) is known to have anti-inflammatory effects. This study aimed to determine whetherchitosan nanoparticles containing mahkota dewa leaf extract would yield superior anti-inflammatory effects in the colon of a mouse model of dextransodium sulfate (DSS)-induced ulcerative colitis, compared with ethanol extract alone after testing the acute toxicities (lethal dose) of both preparations.Methods: For acute toxicity testing, 10 Sprague-Dawley rats were administered 6000 mg/kg body weight (BW) of leaf extract alone or with nanoparticles.Subsequently, mice were divided into the following six groups to determine the anti-inflammatory effects: Untreated, negative control (DSS 2% w/v), leafextract at 12.5 or 25 mg/kg BW, and leaf extract in chitosan nanoparticles at 6.25 or 12.5 mg/kg BW. To induce colitis, DSS (2% w/v) was administeredthrough drinking water for 6 weeks. The anti-inflammatory effect was observed histopathologically by imaging the inflammatory cells of the mice colonwith hematoxylin-eosin (HE) staining.Results: For acute toxicity testing, 10 Sprague-Dawley rats were administered 6000 mg/kg BW of leaf extract alone or with nanoparticles. Subsequently,mice were divided into the following six groups to determine the anti-inflammatory effects: Untreated, negative control (DSS 2% w/v), leaf extract at12.5 or 25 mg/kg BW, and leaf extract in chitosan nanoparticles at 6.25 or 12.5 mg/kg BW. To induce colitis, DSS (1% w/v) was administered throughdrinking water for 6 weeks. The anti-inflammatory effect was observed histopathologically by imaging the inflammatory cells of the mice colon withHE staining.Conclusion: Chitosan nanoparticles containing mahkota dewa leaf extract can be included in the practically non-toxic class of materials. However, anethanol extract of mahkota dewa leaf effectively inhibited DSS-induced inflammation in the mouse colon, regardless of delivery vehicle.

scholarly journals EFFECT OF THE WATER EXTRACT OF THE FOUR O’CLOCK HERB (MIRABILIS JALAPA L.) ON THE HEALING OF OPEN WOUNDS IN RATS

2018 ◽  
Vol 10 (1) ◽  
pp. 155
Author(s):  
Puspita Puspasari ◽  
Fadlina Chany Saputri
Keyword(s):  
Wound Healing ◽  
Water Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Mirabilis Jalapa ◽  
Hematoxylin And Eosin ◽  
Iodine Dose

Objective: This study aimed to analyze the effect on wound healing following treatment with a water extract of Mirabilis jalapa L. by investigatingwound contraction and the associated histopathological changes in rat skin.Methods: Male Sprague-Dawley rats were divided into five groups, namely negative control, positive control (povidone-iodine), dose 1, dose 2, anddose 3. A 20-×10-mm rectangular wound area was created for the test. In dose 1, 2, and 3 groups, the corresponding dose variation of a 0.5-mLM. jalapa L. water extract (dose 1: 5% v/v, ≈243.1 mg/kg body weight BW; dose 2: 10% v/v, ≈486.2 mg/kg BW; and dose 3: 20% v/v, ≈972.4 mg/kg BW)was topically applied for 14 days on open wounds of rats. Widespread wound contractions were measured on days 1, 3, 5, 7, 9, 11, and 13, andhistopathological changes in the skin were observed on day 15 using hematoxylin and eosin staining.Results: The M. jalapa L. water extract accelerated wound healing. The optimal dose was found to be 20% v/v (≈972.4 mg/kg BW).Conclusion: M. jalapa L. extracts are potential healing agents for open wounds.

SERUM 5α-ANDROSTANE-3α,17β-DIOL, ANDROSTERONE AND TESTOSTERONE CONCENTRATIONS IN THE IMMATURE MALE RAT: INFLUENCE OF TIME OF DAY

1977 ◽  
Vol 75 (1) ◽  
pp. 177-178 ◽  
Author(s):  
W. H. MOGER ◽  
P. R. MURPHY
Keyword(s):  
Serum Testosterone ◽  
Temporal Variations ◽  
Time Of Day ◽  
Male Rats ◽  
Male Rat ◽  
Sprague Dawley ◽  
Circadian Variations ◽  
Adult Rat ◽  
Immature Male ◽  
Sprague Dawley Rats

Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada, B3H 4H7 (Received 22 April 1977) The concentration of testosterone in the serum of the adult rat varies significantly over a 24 h period (Kinson & Lui, 1973; Howland, 1975; Wilson, McMillan, Seal & Ahmed, 1976). However, studies on circadian variations in serum testosterone concentrations in immature male rats have yielded conflicting results. Grotjan & Johnson (1976) reported significant changes with time in 25–26-day-old Holtzman rats, whereas Döhler & Wuttke (1976) did not observe significant changes in 13–18 or 25–30-day-old Sprague–Dawley rats maintained on the same lighting schedule (14 h light: 10 h darkness). Recently we reported that from 20–35 days of age, 5α-androstane-3α,17β-diol (androstanediol) is the predominant androgen in the circulation of male rats (Moger, 1977). This study was undertaken to determine temporal variations in the concentrations of androstanediol, androsterone and testosterone. Forty-nine male Sprague

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