scholarly journals KAJIAN IN VITRO SUBTITUSI KONSENTRAT DENGAN PENGGUNAAN LIMBAH PERKEBUNAN SINGKONG YANG DISUPLEMENTASI KOBALT (Co) DAN SENG (Zn) DALAM RANSUM DOMBA

2015 ◽  
Vol 39 (2) ◽  
pp. 71
Author(s):  
Iman Hernaman ◽  
Atun Budiman ◽  
Siti Nurachman ◽  
Kundrat Hidrajat
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
In Vitro Study ◽  
Normal Range ◽  
Native Grass ◽  
Concentrate Supplementation ◽  
Randomized Design ◽  
Completely Randomized Design

<p>This research was aimed to investigate the effect of concentrate substitution with cassava plantation waste supplemented with cobalt and zinc in sheep ration. In vitro study was used in this experiment. Collected data were analyzed by Duncan’s test from Completely Randomized Design with four treatments and four replications. The experiment rations were R1 = 50% native grass + 50% concentrate, R2 = 50% native grass + 50% concentrate + 5 ppm cobalt + 30 ppm zinc, R3 = 50% native grass + 50% cassava plantation waste, R4 = 50% native grass + 50% cassava plantation waste + 5 ppm cobalt + 30 ppm zinc. The results showed that utilization of concentrate increased volatile fatty acid and N-NH3 concentration, and digestibility of dry and organic matter compared with cassava plantation waste. Volatile fatty acid and N-NH3 in sheep ration containing cassava plantation waste were still in normal range with dry and organic matter digestibility were up to 50%, but supplementation of cobalt and zinc had the same effect (P&gt;0.05). It is concluded that cassava plantation waste can be used as sheep feed, but did not substitute concentrate. Supplementation of cobalt and zinc was not effective to improve fermentability and digestibility of cassava plantation waste.</p><p><br />(Key words: Cassava plantation waste, Digestibility, In vitro, N-NH3, Sheep, Volatile fatty acid)</p>

scholarly journals Fermentabilitas dan Kecernaan Ransum Domba yang Mengandung Limbah Roti secara In Vitro

2019 ◽  
Vol 19 (2) ◽  
pp. 85-90
Author(s):  
Asep Saripudin ◽  
Shena Nurpauza ◽  
Budi Ayuningsih ◽  
Iman Hernaman ◽  
Ana Rochana Tarmidi
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Native Grass ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Close Relationship ◽  
Completely Randomized Design ◽  
Organic Matter Digestibility

ABSTRAK. Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum domba yang mengandung limbah roti. Penelitian menggunakan rancangan acak lengkap dengan lima macam ransum perlakuan yang terdiri atas 40% rumput lapangan dan 60% konsentrat yang masing-masing mengandung limbah roti sebanyak 0 (kontrol), 10, 20, 30, dan 40%. Masing-masing perlakuan diulang sebanyak 4 kali dan data yang terkumpul dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Ransum perlakuan dievaluasi secara in vitro. Peubah yang diukur adalah asam lemak terbang (ALT), N-NH3, kecernaan bahan kering, dan kecernaan bahan organik. Hasil penelitian menunjukkan bahwa penggunaan limbah roti dalam ransum domba sampai 40% mampu meningkatkan konsentrasi ALT (102,63 - 143,88 mM), kecernaan bahan kering (64,66 - 78,61%) dan kecernaan bahan organik (53,41 - 65,82%). Sementara itu, terjadi kenaikan konsentrasi N-NH3 (3,87 - 4,90 mM) sampai penggunaan limbah roti 30%, namun penggunaan 40% limbah roti sama dengan ransum kontrol. Konsentrasi ALT memiliki hubungan erat dengan kecernaan bahan kering dan bahan organik dengan nilai r = 0,67 dan 0,65, pada persamaan regresi Y = 0,1591X + 50,79 dan Y = 0,1528X + 39,619. Kesimpulan, Penggunaan limbah roti di dalam ransum sebanyak 40% tidak mengganggu fermentabilitas dan kecernaan ransum secara in vitro.  (In vitro fermentability and digestibility of sheep rations containing bread waste)ABSTRACT. The study aimed to determine the fermentability and digestibility of sheep rations containing bread waste. The study used a completely randomized design with five types of treatment rations consisting of 40% native grass and 60% concentrate and each treatment containing bread waste as much as 0 (control), 10, 20, 30, and 40%. Each treatment was repeated 4 times and the data collected was analyzed by analysis of variance and continued by Duncan test. The treatment ration was evaluated by in vitro. The variables measured were volatile fatty acids (VFA), N-NH3, dry matter digestibility, and organic matter digestibility. The results showed that the use of bread waste in sheep rations up to 40% was able to increase the concentration of VFA (102.63 to 143.88 mM), dry matter (64.66 to 78.61%) and organic matter digestibility (53.41 to 65.82%). Meanwhile, there was an increase in the concentration of N-NH3 (3.87 to 4.90 mM) until 30%, but the use of 40% bread waste was the same as the control ration. The VFA concentration has a close relationship with the dry matter and organic matter digestibility with values of r = 0.67 and 0.65, in the regression equation Y = 0.1591X + 50.79 and Y = 0.1528X + 39.619. The conclusion is using of bread waste as much as 40% did not disturb the fermentability and digestibility of the ration in vitro.

scholarly journals The Effect of Multistage Ammoniation on Fiber Fraction and Digestibility of Maize Stover in vitro

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
K. Dakaew ◽  
A. Abrar ◽  
A. Cherdthong
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Total Volatile ◽  
Maize Stover ◽  
Dry Matter Digestibility ◽  
Fiber Fraction ◽  
Total Volatile Fatty Acid

The main objective of this experiment was to assess the effect of multistage ammoniation on fiber fraction and digestibility of maize stover in vitro. Maize stover was treated by a multistage technique of different urea connect ration (8%, 4%, and 2%) and incubation for 14 days at room temperature.Digestibility of NDF, ADF, ADL, hemicellulose, and cellulose, in vitro dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N, total volatile fatty acid (VFA) and pH concentration were observed. However treatment maize stover and multistage ammoniation maize stover were composed by using T-test student as the statistic. There were not significantly different on fiber fraction and digestibility of maize stover. However, multistage ammoniation decreased ADF fraction by 56.67% to45.39%. The DM digestibility of multistage ammoniation on fiber fraction and digestibility of maize stover also increased by 35.18 to 45.91. The total VFA and N-ammonia of multistage ammoniation on fiber fraction and digestibility of maize stover also higher than control.Keywords: Ammoniation, Digestibility, in vitro, Maize stover

scholarly journals KECERNAAN IN VITRO SILASE SAMPAH SAYUR DAN DAUN GAMAL MENGGUNAKAN MIKROORGANISME RUMEN KAMBING

2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Dry Matter ◽  
Vegetable Waste ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.

Effect of treatment with sodium hydroxide on the feeding value of oat and rape straw for sheep

1985 ◽  
Vol 36 (4) ◽  
pp. 623 ◽  
Author(s):  
JB Coombe ◽  
JG Mulholland ◽  
RI Forrester
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Rape Straw ◽  
Rumen Ph ◽  
Volatile Fatty Acid Concentration ◽  
N Utilization ◽  
Feeding Value ◽  
Naoh Treatment

Oat and rape straws were treated with NaOH (70 and 40 g kg-1, respectively) by spraying with NaOH solutions (20 or 12.5% w/w), consolidating the sprayed straw into stacks, and storing under plastic. After storage for up to 60 weeks, in vitro digestibilities had been approximately doubled, although it appeared that the reaction was complete by 4 weeks. Potential digestibility of straw organic matter estimated by a polyester bag technique, was increased by c. 0.1 by NaOH treatment but the rate of digestion in the rumen was not changed. Following storage, treated and untreated straws were dried, ground, mixed with supplements and pelleted for feeding to sheep. Mixtures of treated or untreated oat straw, minerals, c. 3% urea and 0, 14, 28 or 42% starch were fed to mature wethers. The main effects of NaOH treatment of straw were to increase water intake, digestibilities of organic matter and cellulose, and rumen volatile fatty acid levels, and decrease rumen ammonia levels. Low intakes of NaOH-straw with no added starch were associated with high rumen pH (> 7.5 , probably due to unreacted alkali (equivalent to 23 g NaOH kg-1). Inclusion of starch, but not NaOH-straw treatment, improved dietary N utilization, although mean N balances were negative (- 5.5 to - 0.1 g day-1) on all diets. Feeding treated straw increased the amounts of Na eaten, and of Na and P apparently absorbed; the extra absorption was accounted for by increased urinary excretion so that retention was not increased. When diets of rape straw supplemented with urea and minerals were fed to 2-year-old wethers, NaOH treatment of straw increased intakes of food and water, organic matter digestibility, rumen volatile fatty acid concentration and N balance.

scholarly journals Pengaruh Imbangan Jerami Padi, Dedak Padi dan Onggok Terfermentasi terhadap Kecernaan dan Produk Fermentasi Rumen Secara In Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 59-63
Author(s):  
S.N.O. Suwandyastuti ◽  
Efka Aris Rimbawanto ◽  
Ning Iriyanti
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Rice Straw ◽  
Rice Bran ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Industrial Wastes ◽  
Optimal Ratio ◽  
Dry Matter Digestibility

The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.

scholarly journals EVALUASI KECERNAAN BAHAN KERING, BAHAN ORGANIK, DAN PROTEIN KASAR TONGKOL JAGUNG FERMENTASI DENGAN PENAMBAHAN SUMBER KARBOHIDRAT YANG BERBEDA SECARA IN - VITRO

2020 ◽  
Vol 17 (1) ◽  
pp. 45
Author(s):  
Tri Astuti
Keyword(s):  
Organic Matter ◽  
Rice Bran ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Digestibility ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tapioca Flour ◽  
Different Sources

This research aimed to study effect of Corn cob fermentation using Panerochaeta chrysosporium with adding the different sources of carbohydrat on the digestibility of dry matter, organic matter, and crude protein by in-vitro methode. A Completely Randomized Design  with 4 treatments and  4 replications. The treatments  were : P0 = fermented corncobs + without carbohydrt, P1 = fermented corncobs + 10% rice bran; P2 = fermented corncobs + tapioca flour 10%; P3 = corncob fermented + molasses 10%. The results of this study shown a very significant affected  (P <0.01) on the digestible of dry matter and organic matter but no affected (P> 0.05) on crude protein digestibility. The best results  digestibility of dry matter (66.98%) on the  P3 treatment adding  molasses, and  the highest digestibility of organic matter (67.49%) on the P3 treatment too, but the highest digestibility of  crude protein (51.37%)  in P1 treatment (adding by rice bran)

scholarly journals Evaluación de variedades e híbridos de maíz (Zea mays) para ensilado.

2006 ◽  
Vol 12 (2) ◽  
pp. 193
Author(s):  
Jesús Fuentes ◽  
Antonio Cruz ◽  
Lorenzo Castro ◽  
Gilberto Gloria ◽  
Sergio Rodríguez ◽  
...  
Keyword(s):  
Zea Mays ◽  
Organic Matter ◽  
Protein Content ◽  
Dry Matter ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Fodder Production ◽  
Completely Randomized Design ◽  
New Research

This research was conducted to evaluate 16 corn varieties cultivated for silage. The evaluation included: fodder production of green and dry matter, protein content and in vitro dry matter digestibility (IVDMD) and organic matter digestibility (IVOMD). A completely randomized design was used for statistical analysis and Tukey mean separation was used when appropiated. Fodder production as kg/ha was higher for AN446 (114028), AN445 (110993), and AN430R (106389). Dry matter fodder production was higher for AN447 (29270), AN461 (28026), VS373 (25434). Protein content (%) was higher for G4657 (7.58), AN388 (7.28), and AN448 (7.17). The best values for IVDMD (%)were for G1990 (69.14), AN446 (63.78), and AN430RR (63.50), while the higher values for IVOMD (%) were for G1990 (71.78), AN446 (65.20), and AN447 (65.12). Varieties AN446 and AN447 appeared frequently with the best values in the parameters evaluated, therefore, new research has to be performed with these varieties to confirm results found in this study.

scholarly journals Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

2020 ◽  
Author(s):  
Rittikeard Prachumchai ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Cassava Root ◽  
Randomized Design ◽  
Completely Randomized Design

The current work aimed to screen the ruminal cyanide-utilizing bacteria and evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, kinetics of gas, in vitro degradability, and ruminal fermentation. The experiment was conducted in a Completely randomized design (CRD) for a screening of cyanide-utilizing bacteria and the dietary treatments were the level of cyanide at 0, 150, 300, and 450 ppm. A 5 &times; 3 factorial arrangement in a Completely randomized design was used for in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of 0.5 g dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. Adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p &lt; 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm, resulted in an increase in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. Increasing the FCR level to more than 260 g/kg of 0.5 g substrate could increase cumulative gas production (p &lt; 0.05), whereas increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g/kg PELFUR (p &lt; 0.05). Cyanide concentration in rumen fluid decreased with PELFUR (p &lt; 0.05) supplementation. Degradability of in vitro dry matter and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g/kg PELFUR (p &lt; 0.05) in 440 g/kg FCR. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid, as well as the ratio of C2 to C3 among supplementations with FCR (p &lt; 0.05) were significantly different. As the proportion of FCR increased to 530 g/kg of the substrate, the volume of C3 increased by 14.6%. This is the first finding of bacteria in the rumen capable of utilizing cyanide, and cyanide might function as a nitrogen source for bacterial cell synthesis. Inclusion of FCR of 530 g/kg with 30 g/kg PELFUR could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.

scholarly journals KECERNAAN RANSUM SAPI BALI DENGAN KONSENTRAT FERMENTASI BERBASIS LUMPUR SAWIT DAN BAHAN PAKAN LOKAL

AGROINTEK ◽  
2017 ◽  
Vol 11 (2) ◽  
pp. 63
Author(s):  
Irma Badarina ◽  
Jarmuji Jarmuji ◽  
Dodi Permadi Gultom
Keyword(s):  
Organic Matter ◽  
Palm Oil ◽  
Dry Matter ◽  
Live Weight ◽  
Oil Sludge ◽  
Coffee Husk ◽  
Concentrate Supplementation ◽  
Randomized Design ◽  
Completely Randomized Design

The aim of this research was to evaluate the dry matter and organic matter<br />digestibility in vivo of Bali cattle diet that supplemented with fermented concentrate ration based from palm oil sludge and several local feed sources. Fifteen Bali cattles with 2 years old and the range of live weight 184,5±3,63 kg were distributed into three treatments and five replication in Completely Randomized design. The treatment was the level of concentrate supplementation, They were P1 (10kg/day), P2(7,5kg/day) and P3(5kg/day). The forage and the rice straw were adjusted as much as 10 kg/day and 1 kg/day, respectively. The composition of fermented concentrate consisted of palm oil sludge (70%), rice bran (10%), coffee husk (10% ) and coconut press fiber (10%). As bioactivator used”Bionak”as much as 0,3%. The an aerob fermentation took time a week. The result showed that up to the highest level (10kg/day) the supplementation of fermented concentrate ration enhanced the consumption and the digestibility of the whole rations.

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