scholarly journals Does Ginger Protect Against Ovarian Damage in Rats Induced by Sodium Metabisulfite Toxicity? An Experimental Evidence

2020 ◽  
Vol 14 (4) ◽  
pp. 215-220
Author(s):  
Shahnaz Shekarforoush ◽  
◽  
Nahid Rezaee ◽  
Elaheh Farzanfar ◽  
◽  
...  
Keyword(s):  
Structural Changes ◽  
Ovarian Tissue ◽  
Zingiber Officinale ◽  
Pharmaceutical Products ◽  
Simultaneous Increase ◽  
Control Group ◽  
Sodium Metabisulfite ◽  
Concomitant Treatment ◽  
Ginger Extract ◽  
Food Preservatives

Background: Sulfites including Sodium Metabisulfite (SMB) are commonly used as food preservatives and pharmaceutical products. Despite their worldwide use, there is evidence suggesting their toxicity on human organs and tissues. The purpose of this study was to evaluate the effect of SMB with or without Zingiber officinale (ginger) extract on the rat ovary.  Methods: A total of 32 adult, female Wistar rats were divided into four groups of eight each. They consisted of, a) control group, b) ginger group (500 mg/kg/day), c) SMB group (260 mg/kg/day), and d) combined SMB and ginger group at identical doses. After 28 days, the rats were sacrificed and the ovarian tissue Malondialdehyde (MDA), as a marker of lipid peroxidation was measured. The volume and weight of the ovaries and the number of follicles at different stages were counted by stereological methods.  Results: The SMB treatment caused a significant decrease in the ovarian volume and the number of follicles with simultaneous increase in the number of degenerate follicles (P≤0.001) and MDA level (P≤0.01). Ginger treatment of the rats exposed to SMB significantly increased the number of follicles at various stages and partially reversed the ovarian tissue level of MDA, compared to that in the control group (P≤0.05). Conclusion: The SMB treatment induced structural changes in the rats’ ovaries and the concomitant treatment with ginger largely reversed the damages caused by SMB.

scholarly journals Effect of Sodium Metabisulfite on Rat Ovary and Lipid Peroxidation

2016 ◽  
Vol 10 (2) ◽  
pp. 23-28
Author(s):  
Nahid Rezaee ◽  
◽  
Zahra Nematollahi ◽  
Shahnaz Shekarforous ◽  
Ebrahim Hoseini ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Structural Changes ◽  
Ovarian Tissue ◽  
Critical Role ◽  
Food Additives ◽  
Dietary Factors ◽  
Female Rats ◽  
Control Group ◽  
Sodium Metabisulfite ◽  
Ovarian Volume

Background: Many health problems are related to lifestyle and dietary factors. Since ancient times, food additives such as sulfites have been used to preserve foods. Diverse effects of sulfites on multiple organs have been reported but its effect on female reproductive organ has not been fully elucidated. The aim of this study was to investigate the effects of sodium metabisulfite (SMB) on ovarian tissue in adult rats. Methods: Four groups of female rats (n=32) were used. The experimental rats received 10, 100 and 260 mg/kg SMB for 28 days (S10, S100 and S260 groups, respectively). The control rats received distilled water for the same period. The ovarian volume, weight and the number of different types of follicles were estimated by stereological methods. Lipid peroxidation is assessed indirectly by the measurement of malondialdehyde (MDA), using the thiobarbituric acid (TBA) method. Results: The results showed a significant decrease in the ovarian volume, the number of primordial, primary, secondary, grafian follicles and corpus luteum in the SMB-treated animals compared with the control group (P < 0.05). In comparison to the control group, the number of atretic follicles increased in the SMB-treated rats. MDA was significantly increased in S260 group compared to the control group. Conclusion: The present data confirm sulfite-induced structural changes in the ovary. Increased level of MDA because of SMB ingestion suggests that free radicals may have a critical role in these changes.

scholarly journals Investigation of ginger (Zingiber officinale) aqueous extract as an anti-diabetic in vitro

2021 ◽  
Vol 913 (1) ◽  
pp. 012108
Author(s):  
P Pakan ◽  
K Lidia ◽  
M Riwu
Keyword(s):  
Side Effects ◽  
Glucose Uptake ◽  
Aqueous Extract ◽  
Zingiber Officinale ◽  
Control Group ◽  
Plain Water ◽  
Ginger Extract ◽  
Mouse Tissues ◽  
Treatment Of Diabetes

Abstract Diabetes mellitus is a condition of metabolic imbalance, indicated by a high level of blood glucose (hyperglycemia) resulting from a reduction of insulin secretion, action, or both. People with diabetes suffer from a lack or deficiency of insulin or insulin resistance. The metabolic imbalances are often not satisfactorily corrected using conventional medicines and even cause some side effects, which can be detrimental. Research on herbal medicines for the treatment of diabetes is urged by the need to reduce unwanted side effects common with conventional medicines/treatments used in glucose regulation. This study aims to investigate the antidiabetic effect of Ginger (Zingiber officinale) aqueous extract in improving the glucose uptake in mouse tissues in vitro. This study is a true experimental research design with a posttest-only control group design. There were three groups of mice in this study: the control group, which were only given plain water; the second group of mice with 5% aqueous ginger extract and the last group were given 25% aqueous ginger extract. All groups were given treatment for four consecutive weeks, then dissected their cardiac muscle, skeletal muscle, pancreas, and liver tissues to analyze the glucose uptake. The result showed that both the ginger aqueous extract groups were able to increase the glucose uptake of the mice. In conclusion, this research has shown that aqueous ginger extract may have improved the glucose uptake in most tissues of the mice in the groups. Therefore, ginger could have great potential as an alternative way in the treatment of diabetes type 2.

scholarly journals PENGARUH PEMBERIAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) TERHADAP JUMLAH SEL MAKROFAG ULKUS TRAUMATIKUS MUKOSA MULUT AKIBAT BAHAN KIMIAWI Penelitian in vivo pada Rattus Norvegicus

2015 ◽  
Vol 2 (1) ◽  
pp. 51
Author(s):  
Fitria Hidayati ◽  
Prima Agusmawanti ◽  
Muhammad Dian Firdausy
Keyword(s):  
Oral Mucosa ◽  
Rattus Norvegicus ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Ginger Extract ◽  
Anova Test ◽  
Mucosal Ulceration ◽  
Red Ginger

Background: Traumatic ulcer is an oral mucosa ulcerative lesion caused by trauma. One of the causes is chemicals. Clinical features of traumatic ulcer including yellowish white spots with reddish halo and painful. Treatment for this ulcer are suppressing inflammation and pain thus will increase healing process. Macrophage will be decreased at the end of the inflammatory phase. Red ginger extracts (Zingiber officinale var. Rubrum) contains some compounds that can heal ulcers. Purpose: The objective of this research was to find out the effect of red ginger extract on the macrophage numbers in traumatic oral mucosal ulceration due to chemical in Rattus norvegicus. Method: This study was experimental which post test only control group design. There were five groups: negative control group without intervention, positive control group treat aloclair, and red ginger extract in 2%, 4%, 8% consentration group. The experimental animals were used 25 Rattus norvegicus. They were injured on oral mucosa using H2O2 30% two times a day. Their oral mucosa were made as hystologycal slide, and macrophage counts were observed, then analyzed by Anova test and LSD test. Result: The result indicates some significant effect of red ginger extract on the macrophage numbers in traumatic oral mucosal ulceration from Anova test 0,001 (<0,05). There are significant differences between 2% consentration of red ginger extract and positive control group.There are no significant difference beetween 4%, 8% consentration of red ginger extract and positive control group. Conclusion: It can be concluded that red ginger extract have influence on the number of macrophages in chamical traumatic oral mucosal ulceration in Rattus norvegicus.

scholarly journals PENGARUH PEMBERIAN EKSTRAK ETANOL JAHE MERAH (Zingiber Officinale Roxb var Rubrum) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) YANG DIINDUKSI PARAQUAT DIKLORIDA

2019 ◽  
Vol 5 (2) ◽  
pp. 47-54
Author(s):  
Dian Anggraini ◽  
Sutyarso Sutyarso ◽  
M. Kanedi ◽  
Hendri Busman
Keyword(s):  
Body Weight ◽  
Mus Musculus ◽  
Sperm Count ◽  
Zingiber Officinale ◽  
The Body ◽  
Test Material ◽  
Control Group ◽  
Ginger Extract ◽  
Wide Range ◽  
Red Ginger

Paraquat is one of the chemicals that contributes to increasing pollution Indonesia. The careless use of paraquat can increase production of ROS (Reactive Oxygen Species), resulting in damage to a wide range of vital organs and reproductive system disorders such as infertility. ROS in the body can be captured by antioxidants. Red Ginger contains high antioxidants because there are active phenolic compounds such as gingerol, shagaol, zingeron, ginggerdiol, and zingibren which are proven to protect body cells from damage caused by ROS. This study was aimed to test the effectiveness of red ginger extract (Zingiber officinale Roxb. Var. Rubrum) on the quantity and quality of sperm in male mice (Mus musculus L.) induced by paraquat dichloride.This research uses a completely randomized design and is divided into six treatment groups with each four replications: K1 as the control group (only given H2O); K2 (paraquat induced a dose of 20 mg/kg Body Weight (BW) without the administration of the test substances); K3; K4; K5 (paraquat induced a dose of 20 mg/kg Body Weight (BW) and was given a red ginger extract at a dose of consecutive succession 6 mg / ml, 12 mg / ml, 18 mg / ml) and K6 (Only given a test material the red ginger extract as much as 18 mg / ml). Paraquat was given 2 times a week for 21 days and the red ginger extract was given for 35 days. The results of analysis by One-way ANOVA followed LSD at 5% significance level showed the ethanolic extract of red ginger can increase the sperm count, motility, viability and Morphology sperm of mice induced by the paraquat diklorida.

scholarly journals Organ-Protective Effect of Red Ginger Extracts in Male Mice Induced by Paraquat

2019 ◽  
Vol 6 (2) ◽  
pp. 8-17
Author(s):  
Kamiliah Tsany ◽  
Sutyarso Sutyarso ◽  
M. Kanedi ◽  
Hendri Busman
Keyword(s):  
Ethanol Extract ◽  
Zingiber Officinale ◽  
The Body ◽  
Control Group ◽  
Male Mice ◽  
Ginger Extract ◽  
Randomized Design ◽  
Significant Difference ◽  
Lung Histology ◽  
Red Ginger

Paraquat is a toxic compound that disrupts biochemical processes in the body, cell death, and multi-organ failure. The liver and lung are the target organ of paraquat toxicity. The body needs antioxidants to counteract free radicals. Red ginger (Zingiber officinale Roxb. Var rubrum) is a spice that contains antioxidants. This study was conducted to determine the effect of ethanol extract of red ginger (Zingiber officinale Roxb. Var rubrum) on liver and lung histology male mice induced by paraquat. The research used completely randomized design and use of 6 treatment groups, i.e. control group (given distilled water and standard feed), PQ group (induced by paraquat 20 mg/kg per BW), group P1 (given paraquat and ethanol extracts of red ginger 200 mg/kg per BW), group P2 (given paraquat and ethanol extract of red ginger 400 mg/kg per BW), group P3 (given paraquat and ethanol extract of red ginger 600 mg/kg per BW), group P4 (given ethanol extract of red ginger extract 600 mg/kg per BW). The data obtained will be tested using One Way ANOVA and continued by Least Significant Difference (LSD) with a 95% confidence level. The results showed ethanol extract of red ginger effective against liver and lung histology male mice induced by paraquat. The results also showed ethanol extract of red ginger with a dose of 400 mg/kg per BW is effective in protecting the liver and lung from damage caused induced by paraquat.

scholarly journals The Effect of Ginger (Zingiber officinale) on Diet Induced Hyperlipidemia and Tissue Histology in Adult Female Wistar Rats: A Biochemical and Histopathological Study

2020 ◽  
pp. 1-8
Author(s):  
Olufunke R. Akanfe ◽  
Ibukunoluwa A. Komolafe ◽  
Ayobola A. Iyanda
Keyword(s):  
High Fat Diet ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Zingiber Officinale ◽  
Staining Technique ◽  
Control Group ◽  
Histopathological Study ◽  
Distilled Water ◽  
High Fat ◽  
Ginger Extract

The study is aimed at determining the atheroprotective property of ginger (Zingiber officinale) aqueous extract to rats fed with a high-fat diet. Each group consisted of 5 rats. Group 1 represent the control group and received normal rat pellets; groups 2-5 were maintained on high-fat diet for 6 weeks to establish hyperlipidemia. Groups 1, 2, 3, 4 and 5 received distilled water, distilled water, 400, 500 and 600 mg/kg (body weight) Zingiber officinale respectively for 4 weeks. Serum concentrations of triglycerides (TG), total cholesterol (TC) and high-density lipoprotein-cholesterol (HDL-C) were determined using standard biochemical methods. Histologic examination of tissues was by Hematoxylin-Eosin staining technique. Data were analyzed using Student’s t-test and P<0.05 was considered significant. A decrease in the mean concentrations of TG, TC, low-density lipoprotein (LDL-C) and increase in HDL-C concentration of each of the treatment groups (3, 4, 5) were observed when compared with that of group 2. In rats fed high-fat diet, histologic abnormalities were observed for the heart, liver, and kidney which were not adequately corrected by ginger treatment. Results suggest that at dosage levels studied, aqueous ginger extract ameliorated biochemical but not histologic consequences of the high-fat diet.

scholarly journals PHARMACOLOGICAL STUDY OF OINTMENT BASED ON ZINGIBER OFFICINALE EXTRACT

2021 ◽  
Author(s):  
Lidiia Eberle ◽  
◽  
Alona Kobernik ◽  
Keyword(s):  
Medicinal Plants ◽  
Diclofenac Sodium ◽  
Zingiber Officinale ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Control Group ◽  
Ginger Extract ◽  
Inflammatory Reactions ◽  
Anti Inflammatory ◽  
Active Substances

Nonsteroidal anti-inflammatory drugs such as ibuprofen and diclofenac sodium are widely known drugs for the treatment of inflammatory reactions. Prolonged use of drugs inevitably leads to the development of adverse reactions and reduced immunity of patients. Since the reduction of the "drug load" is an urgent task in this situation, an alternative direction to solve it is the use of natural therapeutic factors, the rehabilitation capabilities of which in these conditions are very appropriate and effective. There is a whole arsenal of medicinal plants in the world, they contain a complex of biologically active substances and are not inferior in therapeutic properties to synthetic agents. Among such medicinal plants is Zingiber officinale, which for the past 10 years is actively grown in Ukraine in the city of Kherson. The study shows the relevance of making a mild drug based on a thick extract of the rhizome of Zingiber officinale. It is proved that the extract belongs to low-toxic compounds, which allows its use in the composition of drugs for transdermal administration. An ointment was made on the basis of a thick ginger extract with a content of biologically active substances of 0.025%. The content was standardized according to the content of the sum of polyphenolic compounds. Inflammation was induced by subplantar injection to the plantar fasciitis (aponeurosis) of the hind limb of rats using 30 µL AITC solution (100 µg/limb) in 1,2-propyleneglycol. The dynamics of changes of inflammatory process was evaluated before addition of the inflammation inducer and after 1, 2, 3, 4, 6 and 24 hours of its injection for measuring the volume and the thickness of affected limb. Ointment based on ginger extract has a high level of anti-inflammatory properties, which is shown on the basis of the positive dynamics of changes in the morphological parameters of the affected limbs of animals. Prophylactic application of the phytopreparation two days before the start of the experiment helped to block the development of inflammation in comparison with the control group and the reference drug – ibuprofen ointment. The soft dosage form created and studied by us based on the thick extract of Zingiber officinale is an effective anti-inflammatory agent that can not only treat inflammation, but also block its development under previous prophylactic applications.

scholarly journals The Histological Effect of Aqueous Ginger Extract on Kidneys and Lungs of Diabetic Rats

2018 ◽  
Vol 10 (4) ◽  
pp. 23
Author(s):  
Maisa.M.A. Al-Qudah ◽  
Ezz Al-Dein Al-Ramamneh ◽  
Moawiya A. Haddad ◽  
Amal Al-Abbadi
Keyword(s):  
Day Treatment ◽  
Zingiber Officinale ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Histological Structure ◽  
Alveolar Wall ◽  
Ginger Extract ◽  
Group I ◽  
Histological Effect

Diabetes is a disorder affecting various-aged humans and which can with time cause serious problems for the patient. Medicinal plants are known for their hypoglycemic effects; and among which is ginger (Zingiber officinale) known also for its culinary uses. This study, therefore, was undertaken to evaluate the histological effect of 21-day treatment of aqueous ginger extract used at 500 mg / kg BW on female diabetic rats. Fifteen female albino rats were divided into three groups; Group I: control, Group II: non-treated diabetic, and Group III: ginger extract-treated diabetic rats. The ginger extract-treated diabetic group received the daily dose orally for three weeks. Results show that organ weight was not significantly changed. Light microscopic examination of 5&micro;m sections of extract-treated Kidney and Lung of the diabetic rats revealed approximately normal histological structure compared with the untreated ones. The normal appearance of glomeruli and alveoli as well as the normal alveolar wall assumed the ameliorative effect ginger aqueous extract could have on kidney and lung of diabetic rats. These results indicated that this dose of ginger extract may be effective in the treatment of diabetic rats.

EVALUATION OF LEFT VENTRICULAR MASS IN THE PREHYPERTENSIVES BY ELECTROCARDIOGRAPHY AND ECHOCARDIOGRAPHY

2011 ◽  
pp. 119-125
Author(s):  
Thi Thuy Hang Nguyen
Keyword(s):  
Left Ventricular Hypertrophy ◽  
Left Ventricular Mass ◽  
Ventricular Hypertrophy ◽  
Structural Changes ◽  
Left Ventricular ◽  
Control Group ◽  
Concentric Left Ventricular Hypertrophy ◽  
Increased Risk ◽  
Lv Mass ◽  
Left Ventricular Morphology

Objective: Prehypertensive individuals are at increased risk for developing hypertension and their complication. Many studies show that 2/3 prehypertensive individuals develop hypertension after 4 years. ECG and echocardiography are the routine tests used to assess LV mass. The objective of the research to determine the percentage of change in left ventricular morphology in the ECG, echocardiography, which explore the characteristics of left ventricular structural changes by echocardiography in pre-hypertensive subjects. Materials and method: We studied a total of 50 prehypertensive, 30 males (60%) and 20 females (40%), mean age 48.20±8.47years. 50 normotensive volunteers as control participants. These subjects were examined for ECG and echocardiography. Results: In prehypertensive group, with 18% of left ventricular hypertrophy on electrocardiogram, 12% of left ventricular hypertrophy on echocardiography; in the control group, we did not find any subjects with left ventricular hypertrophy. In the group with left ventricular hypertrophy, mostly eccentric left ventricular hypertrophy (83.33%), concentric left ventricular hypertrophy is 16.67%. Restructuring of left ventricular concentric for 15.9% of subjects without left ventricular hypertrophy on echocardiography. Conclusion: There have been changed in left ventricular morphology even in prehypertensive

EFEK PENAMBAHAN EKSTRAK AIR JAHE (Zingiber officinale Roscoe) DAN PENYIMPANAN DINGIN TERHADAP MUTU SENSORI IKAN TUNA (Thunnus albacores)

2010 ◽  
Vol 6 (1) ◽  
pp. 36
Author(s):  
Silvana Dinaintang Harikedua
Keyword(s):  
Sensory Quality ◽  
Zingiber Officinale ◽  
The Other ◽  
Ginger Extract ◽  
Other Hand ◽  
Storage Treatment ◽  
Zingiber Officinale Roscoe ◽  
Hedonic Scale ◽  
And Storage

The objective of this study was to investigate the effect of ginger extract addition and refrigerate storage on sensory quality of Tuna through panelist’s perception. Panelists (n=30) evaluated samples for overall appearance and flavor attribute using hedonic scale 1–7. The sample which is more acceptable by panelists on flavor attributes having 3% gingers extract and storage for 3 days. The less acceptable sample on flavor attribute having 0% ginger extract and storage for 9 days. On the other hand, the sample which is more acceptable by panelists on overall appearance having 0% ginger extract without storage treatment. The less acceptable sample on overall appearance having 3% ginger extract and storage for 9 days.

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