PENGARUH PEMBERIAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) TERHADAP JUMLAH SEL MAKROFAG ULKUS TRAUMATIKUS MUKOSA MULUT AKIBAT BAHAN KIMIAWI Penelitian in vivo pada Rattus Norvegicus

Background: Traumatic ulcer is an oral mucosa ulcerative lesion caused by trauma. One of the causes is chemicals. Clinical features of traumatic ulcer including yellowish white spots with reddish halo and painful. Treatment for this ulcer are suppressing inflammation and pain thus will increase healing process. Macrophage will be decreased at the end of the inflammatory phase. Red ginger extracts (Zingiber officinale var. Rubrum) contains some compounds that can heal ulcers. Purpose: The objective of this research was to find out the effect of red ginger extract on the macrophage numbers in traumatic oral mucosal ulceration due to chemical in Rattus norvegicus. Method: This study was experimental which post test only control group design. There were five groups: negative control group without intervention, positive control group treat aloclair, and red ginger extract in 2%, 4%, 8% consentration group. The experimental animals were used 25 Rattus norvegicus. They were injured on oral mucosa using H2O2 30% two times a day. Their oral mucosa were made as hystologycal slide, and macrophage counts were observed, then analyzed by Anova test and LSD test. Result: The result indicates some significant effect of red ginger extract on the macrophage numbers in traumatic oral mucosal ulceration from Anova test 0,001 (<0,05). There are significant differences between 2% consentration of red ginger extract and positive control group.There are no significant difference beetween 4%, 8% consentration of red ginger extract and positive control group. Conclusion: It can be concluded that red ginger extract have influence on the number of macrophages in chamical traumatic oral mucosal ulceration in Rattus norvegicus.

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PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

Biopendix: Jurnal Biologi, Pendidikan dan Terapan ◽

10.30598/biopendixvol1issue2page198-208 ◽

2015 ◽

Vol 1 (2) ◽

pp. 198-208

Author(s):

Nur Fitri

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Leaf Extract ◽

Healing Process ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

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EFEKTIVITAS PEMBERIAN EKSTRAK JAHE MERAH (ZINGIBER OFFICINALE) TERHADAP JUMLAH SEL FIBROBLAS DALAM PROSES PENYEMBUHAN ULKUS PADA MUKOSA MULUT TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

ODONTO Dental Journal ◽

10.30659/odj.3.2.98-104 ◽

2016 ◽

Vol 3 (2) ◽

pp. 98

Author(s):

Prima Agusmawanti

Keyword(s):

Wound Healing ◽

Oral Mucosa ◽

Rattus Norvegicus ◽

Healing Process ◽

White Male ◽

Zingiber Officinale ◽

Male Rats ◽

Wound Healing Process ◽

P Value ◽

Red Ginger

Background: In the wound healing process there are a variety of processes, including the most important are fibroplasia process that has a very important role in the wound healing process. Red ginger (Zingiber officinale) contains active ingredients include a mixture of components of essential oils and resins that oleoresin which has anti-inflammatory effects. The aim is determine the effect of red ginger extract against the number of fibroblasts in healing ulcers on the oral mucosa white male rats (Rattus norvegicus)Method: Samples using 24 male rats 2-3 months of age and body weight of 120-200 grams. Mandibular labial mucosa injured mice using H2O2 solution for 2x1 days. The samples were divided into 4 groups, where each group each of 4individuals: group 1 without any treatment, group 2 was treated application ginger gel extract 4%, group 3 was given treatment application ginger gel extract 8% and group 4 treated with applications aloclair gel. On the fourth day after the injury,the rats were decapitated, then do making preparations for histology to count the number of fibroblast cells.Result: The results Oneway ANOVA p value <0.05 and post Hoc Test LSD p value <0.05 which means that the extract of red ginger significantly affects the number of fibroblast cells and between groups have significant differencesConclusion: Extract red ginger gel is effective against the number of fibroblasts in healing ulcers on the oral mucosa white male rats.

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PENGARUH PEMBERIAN EKSTRAK ETANOL JAHE MERAH (Zingiber Officinale Roxb var Rubrum) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) YANG DIINDUKSI PARAQUAT DIKLORIDA

Jurnal Ilmiah Biologi dan Keanekaragaman Hayati ◽

10.23960/jbekh.v5i2.53 ◽

2019 ◽

Vol 5 (2) ◽

pp. 47-54

Author(s):

Dian Anggraini ◽

Sutyarso Sutyarso ◽

M. Kanedi ◽

Hendri Busman

Keyword(s):

Body Weight ◽

Mus Musculus ◽

Sperm Count ◽

Zingiber Officinale ◽

The Body ◽

Test Material ◽

Control Group ◽

Ginger Extract ◽

Wide Range ◽

Red Ginger

Paraquat is one of the chemicals that contributes to increasing pollution Indonesia. The careless use of paraquat can increase production of ROS (Reactive Oxygen Species), resulting in damage to a wide range of vital organs and reproductive system disorders such as infertility. ROS in the body can be captured by antioxidants. Red Ginger contains high antioxidants because there are active phenolic compounds such as gingerol, shagaol, zingeron, ginggerdiol, and zingibren which are proven to protect body cells from damage caused by ROS. This study was aimed to test the effectiveness of red ginger extract (Zingiber officinale Roxb. Var. Rubrum) on the quantity and quality of sperm in male mice (Mus musculus L.) induced by paraquat dichloride.This research uses a completely randomized design and is divided into six treatment groups with each four replications: K1 as the control group (only given H2O); K2 (paraquat induced a dose of 20 mg/kg Body Weight (BW) without the administration of the test substances); K3; K4; K5 (paraquat induced a dose of 20 mg/kg Body Weight (BW) and was given a red ginger extract at a dose of consecutive succession 6 mg / ml, 12 mg / ml, 18 mg / ml) and K6 (Only given a test material the red ginger extract as much as 18 mg / ml). Paraquat was given 2 times a week for 21 days and the red ginger extract was given for 35 days. The results of analysis by One-way ANOVA followed LSD at 5% significance level showed the ethanolic extract of red ginger can increase the sperm count, motility, viability and Morphology sperm of mice induced by the paraquat diklorida.

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Organ-Protective Effect of Red Ginger Extracts in Male Mice Induced by Paraquat

Jurnal Ilmiah Biologi dan Keanekaragaman Hayati ◽

10.23960/jbekh.v6i2.24 ◽

2019 ◽

Vol 6 (2) ◽

pp. 8-17

Author(s):

Kamiliah Tsany ◽

Sutyarso Sutyarso ◽

M. Kanedi ◽

Hendri Busman

Keyword(s):

Ethanol Extract ◽

Zingiber Officinale ◽

The Body ◽

Control Group ◽

Male Mice ◽

Ginger Extract ◽

Randomized Design ◽

Significant Difference ◽

Lung Histology ◽

Red Ginger

Paraquat is a toxic compound that disrupts biochemical processes in the body, cell death, and multi-organ failure. The liver and lung are the target organ of paraquat toxicity. The body needs antioxidants to counteract free radicals. Red ginger (Zingiber officinale Roxb. Var rubrum) is a spice that contains antioxidants. This study was conducted to determine the effect of ethanol extract of red ginger (Zingiber officinale Roxb. Var rubrum) on liver and lung histology male mice induced by paraquat. The research used completely randomized design and use of 6 treatment groups, i.e. control group (given distilled water and standard feed), PQ group (induced by paraquat 20 mg/kg per BW), group P1 (given paraquat and ethanol extracts of red ginger 200 mg/kg per BW), group P2 (given paraquat and ethanol extract of red ginger 400 mg/kg per BW), group P3 (given paraquat and ethanol extract of red ginger 600 mg/kg per BW), group P4 (given ethanol extract of red ginger extract 600 mg/kg per BW). The data obtained will be tested using One Way ANOVA and continued by Least Significant Difference (LSD) with a 95% confidence level. The results showed ethanol extract of red ginger effective against liver and lung histology male mice induced by paraquat. The results also showed ethanol extract of red ginger with a dose of 400 mg/kg per BW is effective in protecting the liver and lung from damage caused induced by paraquat.

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PENGARUH KONSUMSI KEJU CHEDDAR 10 GRAM TERHADAP pH SALIVA - Studi terhadap Mahasiswa Fakultas Kedokteran Gigi Universitas Islam Sultan Agung Semarang

ODONTO Dental Journal ◽

10.30659/odj.1.1.34-38 ◽

2014 ◽

Vol 1 (1) ◽

pp. 34

Author(s):

Nadia Fitri Hapsari ◽

Ade Ismail ◽

Oedijono Santoso

Keyword(s):

Cheddar Cheese ◽

Positive Control ◽

T Test ◽

Positive Control Group ◽

Control Group ◽

Anova Test ◽

Salivary Ph ◽

Post Hoc ◽

One Way Anova ◽

Paired T Test

Background: Dental caries is the main problem of oral health in the world. The preventive is consumption cheddar cheese with caseinfosfoprotein and calcium. This study aimed to determine whether the consumption of 10 grams cheddar cheese can increase salivary pH. Method: The type of this research method is experimental with pre and post design. The samples in this research were 30 students FKG Unissula who inclusion criteria, 10 people the treatment group (consuming 10 grams of cheddar cheese), 10 people positive control group (consuming chocolate biscuits), and 10 people negative control group. The data analysis techniques using Paired T Test to determine the salivary pH before and after treatment. Furthermore, to know differences among the three groups using One Way Anova Test and Post Hoc Test . Result: Based Test Paired T Test showed that the treatment and negative group increased salivary pH. Positive control group decreased salivary pH. Based on One Way Anova test significant value 0.000 (p≤0.05), it means differences between 3 groups. Based on Post Hoc Test found significant value p≤0.05, it means differences between one group to another. Conclusion: From study result concluded that consumption 10 grams of cheddar cheese can raise the salivary pH.

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Effectiveness of Gout Therapy with Polyherbal Extract of Shallot (Allium ascalonicum L.) and Red Ginger (Zingiber officinale var rubrum) in Hyperuricemia Mice

Jurnal Sain Veteriner ◽

10.22146/jsv.51360 ◽

2020 ◽

Vol 38 (2) ◽

pp. 135

Author(s):

Fathur Rohman Haryadi

Keyword(s):

Uric Acid ◽

Zingiber Officinale ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Acute Gout ◽

Ginger Extract ◽

Oxidase Enzyme ◽

Red Ginger ◽

Potassium Oxonate

Gout can cause inflammation of acute gout arthtritis, as well as other complications. Provision of chemical drugs in the long term have side effects. Shallots (Allium ascalonicum L.) contain flavonoids which can inhibit the xanthine oxidase enzyme, so as to reduce uric acid levels. Red ginger extract (Zingiber officinale var rubrum) is rich in gingerol to reduce uric acid with anti-inflammatory effect. Polyberbal combination is expected to increase the effectiveness of therapy. The research aims to determine the effectiveness of polyherbal combinations. 25 male rats (2-3 months) were divided into five groups, namely negative control, positive control, (P1) 25% red ginger extract: 75% shallot, (P2) 50% red ginger extract: 50% shallot, ( P3) 75% red ginger extract: 25% shallot. Induction of potassium oxonate 250 mg / kg BW intraperitoneally on the 7th day. The first measurement of uric acid on the 14th day with Urid Acid Toos, the 15th to 28th days was administered herbal / chemical drugs according to the group. Measurement of second and third uric acid on days 21 and 28. Data were analyzed by one-way Anova (RAL), further significance tests using Least Significant Different (LSD). The results showed that administration of potassium oxonate and combination of herbal extracts had a significant effect (p <0.05) on uric acid levels in all groups. The most effective herbal combination doses are (P3) 75% red ginger (450 mg / 200 gr) and 25% shallot (150 mg / 200 gr BW). The conclusion of this study is the combination of red ginger extract and shallot can be recommended as a uric acid-lowering agent.

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Efek Pemberian Ekstrak Jahe (Zingiber officinale) terhadap Gambaran Histopatologik Lambung Tikus Wistar yang Diinduksi Asam Asetat

Medical Scope Journal ◽

10.35790/msj.1.2.2020.27826 ◽

2020 ◽

Vol 1 (2) ◽

Author(s):

Monica Ch. Pungus ◽

Magdalena P. Lintong ◽

Maria K. Sambuaga

Keyword(s):

Acetic Acid ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Zingiber Officinale ◽

Positive Control ◽

Arachidonic Acid Metabolism ◽

Peptic Ulcers ◽

Mucosal Layer ◽

Male Wistar Rats ◽

Red Ginger

Abstract: In stomach induced by acetic acid, the gastric acidity may increase leading to irritation of the mucosal layer and imbalance of defense factors and damaging factors. This might lead to the occurrence of gastritis or peptic ulcers. Ginger is rich in flavonoids. In the inflammation process, ginger constituents such as zingerone, zingiberenes, gingerols, and shogaol may be able to inhibit the cyclooxygenation and lipookoxygenase pathways in the arachidonic acid metabolism. This study was aimed to identify the effects of ginger on the histopathological features of stomach induced by acetic acid. This was an experimental laboratory study. In this study, we used red ginger (Zingiber officinale var. rubrum) originated from Tomohon. Samples were male Wistar rats (Rattus norvegicus) divided into 5 groups; one as the positive control. The results showed that groups given ginger had intact surface mucosa, inflammation cells (eosinophils and PMN), and blood vessel dilation. Meanwhile, the positive control showed erosive surface mucosa. Worms were identified in all groups. It is concluded that in Wistar rats, administration of ginger after the stomach induced by acetic acid still showed gastritis feature but without acute erosive gastritis.Keywords: ginger, stomach, gastritis, peptic ulcers, acetic acid, vinegar Abstrak: Pada lambung yang diinduksi dengan asam asetat, kadar keasaman lambung dapat meningkat sehingga mengiritasi lapisan mukosa dan mengakibatkan ketidakseimbangan faktor pertahanan dan faktor perusak lambung. Hal ini dapat menyebabkan gastritis atau ulkus peptikum. Jahe dikenal kaya akan flavonoid. Pada proses inflamasi, konstituen dari jahe seperti zingerone, zingiberenes, gingerols, dan shogaol mampu menghambat jalur siklooksigenasi dan lipooksi-genase pada metabolisme asam arakidonat. Penelitian ini bertujuan untuk mengetahui efek pemberian jahe terhadap gambaran histopatologik lambung yang diinduksi dengan asam asetat. Jenis penelitian ialah eksperimental laboratorik. Pada penelitian ini digunakan jahe merah (Zingiber officinale var. rubrum) yang berasal dari daerah Tomohon. Sampel penelitian ialah 25 ekor tikus Wistar (Rattus norvegicus) jantan, dibagi atas 5 kelompok; satu kelompok sebagai kontrol positif. Hasil penelitian mendapatkan kelompok yang diberikan jahe memperlihatkan permukaan mukosa lambung intak, adanya sel-sel radang (eosinofil dan PMN), serta pelebaran pembuluh darah sedangkan pada kelompok kontrol positif yang hanya diberikan asam asetat tampak permukaan mukosa lambung erosif. Pada semua sampel ditemukan adanya cacing. Simpulan penelitian ini ialah pada tikus Wistar, pemberian jahe setelah lambung diinduksi dengan asam asetat masih menun-jukkan adanya gambaran histopatologik gastritis namun tidak terdapat gastritis erosif akut.Kata kunci: jahe, lambung, gastritis, ulkus peptikum, asam asetat

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Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro

Biomolecular and Health Science Journal ◽

10.20473/bhsj.v3i1.19130 ◽

2020 ◽

Vol 3 (1) ◽

pp. 24

Author(s):

Samira Assegaf ◽

Arthur Pohan Kawilarang ◽

Retno Handajani

Keyword(s):

Antibacterial Activity ◽

Streptococcus Pyogenes ◽

Zingiber Officinale ◽

Positive Control ◽

Bacterial Suspension ◽

Ginger Extract ◽

Mueller Hinton ◽

Red Ginger ◽

Mueller Hinton Broth

Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.

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UJI EFEK ANALGETIK NANOPARTIKEL EKSTRAK RIMPANG JAHE MERAH (Zingiber officinale var Rubrum) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.9.2020.29269 ◽

2020 ◽

Vol 9 (2) ◽

pp. 184

Author(s):

Christian Excelino Kaunang ◽

Widdhi Bodhi ◽

Hosea Jaya Edi

Keyword(s):

Rattus Norvegicus ◽

White Male ◽

Zingiber Officinale ◽

Test Material ◽

Chemical Components ◽

Ginger Extract ◽

Analgesic Effects ◽

Group I ◽

Ginger Rhizome ◽

Red Ginger

ABSTRACTRed Ginger (Zingiber officinale var rubrum) has long been known that its properties can cure various diseases. Chemical components such as gingerol, shogaol, and zigeron that can provide pharmacological and physiological effects such as analgesic, antioxidant, carcinogenic, and non-mutagenic effects even at high concentrations. This study aims to determine whether the nanoparticles of red ginger rhizome extracts can provide analgesic effects in white male wistar rats (Rattus norvegicus). The red ginger rhizome was extracted using maceration method with ethanol as a solvent and nanoparticle formulation of red ginger extract was made using an ionic gelation method. The test mice animals were divided into 5 kinds of treatments and each treatment performed 3 repetitions. Test of the analgesic effect was carried out by the method of thermic induction using waterbath and was observed as many as 5 times, namely before giving test material, the 30th minute, 60th minute, 90th minute, and 120th minute. Group I as negative control was given 1% CMC, group II as positive control was given paracetamol, group III-V was treated using nanoparticles red ginger extract with multilevel doses of 0.0215g, 0.043g, and 0.086g. Based on the ANOVA test, the results obtained of analgesic effects significantly different with p = 0.019 (p < 0.05). Keywords: analgesic, nanoparticles, red ginger. ABSTRAKJahe merah (Zingiber officinale var rubrum) sudah lama diketahui khasiatnya yaitu dapat menyembuhkan berbagai macam penyakit. Komponen kimia seperti gingerol, shogaol, dan zigeron yang dapat memberikan efek farmakologi maupun efek fisiologi seperti efek analgetik , antioksidan, karsinogenik, dan non mutagenik meskipun pada konsentrasi yang tinggi. Penelitian ini bertujuan untuk mengetahui apakah nanopartikel ekstrak rimpang jahe merah dapat memberikan efek analgetik pada tikus putih jantan galur wistar (Rattus norvegicus). Rimpang jahe merah diekstraksi menggunakan metode maserasi dengan etanol sebagai pelarut dan dibuat formulasi nanopartikel ekstrak rimpang jahe merah dengan metode gelasi ionik. Hewan uji tikus dibagi menjadi 5 macam perlakuan setiap perlakuan dilakukan 3 kali pengulangan. Untuk pengujian efek analgetik dilakukan dengan metode induksi termik menggunakan waterbath dan dilakukan pengamatan sebanyak 5 kali yaitu sebelum pemberian bahan uji, menit ke-30, menit ke-60, menit ke-90, dan menit ke-120. Kelompok I sebagai kontrol negatif diberi CMC 1%, kelompok II sebagai kontrol positif diberi parasetamol, kelompok III-V diberi perlakuan menggunakan nanopartikel ekstrak rimpang jahe merah dengan dosis yang bertingkat yaitu 0,0215g, 0,043g, dan 0,086g. Berdasarkan hasil uji ANOVA didapatkan efek analgetik yang berbeda secara bermakna dengan p = 0,019 (p<0,05). Kata Kunci: Jahe merah, analgetik, nanopartikel.

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Resistance to Fracture of Dental Roots Obturated with Different Materials

BioMed Research International ◽

10.1155/2015/591031 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Berkan Celikten ◽

Ceren Feriha Uzuntas ◽

Kamran Gulsahi

Keyword(s):

Fracture Resistance ◽

Testing Machine ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Root Canal Filling ◽

Filling Materials ◽

Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

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