scholarly journals Mineral and lipid composition the body of the honeybees organism and the biological value of honey in the summer-autumn period under the conditions of feeding honey bees by citrate-capped silver and copper nanoparticles

2018 ◽  
Vol 20 (89) ◽  
pp. 89-94
Author(s):  
I. Dvylyuk

The article presents the results of investigations of individual mineral elements – Fe, Zn, Cu, Cr, Co, Pb, Cd in the tissues of the honeybees, common lipids and the ratio of their classes in the body of honey bees and the biological value of honey under the conditions of feeding of honey bees by citrate-capped silver and copper nanoparticlescitrates. The research was conducted on the honey bees of the Carpathian race on the basis of the apiary of the Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv in the summer-autumn period in 5 groups of bee colonies, formed on the principle of analogues, by three bees colony in each group. Control group (I) received a sugar syrup in 1:1 concentration (1000 ml/bee/week). Experimental group (II) additionally added to the syrup Ag in the amount of 0.5 mg/l of sugar syrup, the third experimental group – under similar conditions received Ag in the amount of 1 mg/l, IV group received 0.5 mg of sugar Cu syrup/l and V–Cu in the amount of 1 mg/l of sugar syrup. The trace elements were injected into a sugar syrup in the form of citrates obtained by the nanotechnology method from LLC Nanomaterials and Nanotechnologies, Kyiv. Research period lasted 28 days at a feeding interval of 7 days. According to the research data, certain intergroup differences in the content of mineral elements in the bee organism have been established: the increase of Fe, Co, Cr content in the background of a probable decrease Zn in the samples of the whole body tissues of the IV and V experimental groups. Pb decrease in the content of the tissues of the body of all experimental groups with a high probability in the IV and V experimental groups (P < 0.02) was observed, as well as a tendency to decrease the level of Cd in the tissues of the whole organism in all experimental groups compared with the control group. According to the results of the conducted studies, the growth of the content of common lipids in the whole of the bees is observed and the probable differences in the fractional distribution of lipids are expressed. In particular, in the tissues of bees of all experimental group, higher levels of phospholipids and triacylglycerols were observed against the background of a decrease in the relative content of monodiacylglycerols, cholesterol. Feeding with sugar honey bees by citrate-capped silver and copper nanoparticles in the summer-autumn period was characterized by certain differences in the content of individual trace elements in honey in all experimental groups, in particular, higher content of Fe, Cu, Co in samples of honey IV and V experimental groups and decrease in the concentration of Cr, Pb, Cd. According to physico-chemical parameters, in honey of experimental groups, there were probably higher differences in the content of amino acids of proline, diastase activity, and a decrease in pH, water. The content of proline in samples of honey III and IV experimental groups was higher on 22.6% and 16.9% (P < 0.05–0.001) compared to control. In samples of honey II–V in experimental groups, diastase activity increased in 1.7–2.2 times (P < 0.01–0.001), while fluctuations of pH of honey by 4.0–5.0% (P < 0.001) were comparatively to control. Thereby, feeding with sugar syrup citrate-capped silver and copper nanoparticles, in doses of 0.5 and 1 mg, caused a certain corrective effect on the content of Fe, Zn, Cu, Cr, Co, and antagonistic effects on Pb, Cd in tissues and production of honey bees.

2018 ◽  
Vol 8 (1) ◽  
pp. 420-425 ◽  
Author(s):  
V. Bomko ◽  
Yu. Kropyvka ◽  
L. Bomko ◽  
S. Chernyuk ◽  
S. Kropyvka ◽  
...  

<p><span lang="EN-US">To realize the genetic potential of animals, an important role should be taken to prevent the presence of mineral substances in the rations. Because of their lack of revenue in the body of animals the productivity is decreased, the reproductive function is disturbed, there is a disease. there are diseases.</span></p><p><span lang="EN-US">Material for the scientific and economic experiment on the use of mixed ligand complexes of Zinc, Manganese and Cobalt on the milk productivity of high-yielding cows and the exchange of Mangan in their body were Holstein animals, Ukrainian Black- Spotted dairy and Ukrainian Red - Spotted dairy breeds. The trace elements were introduced into feed concentrates, and they into the fodder mix by the method of weight dosing and multi-stage mixing. Balancing of rations of highly productive cows of the 1st control group to norm only Selenium, Kuprum and Iodine, led to a concentration in 1 kg DM (dry matter) of feed mix, mg: Zinc – 32.4; Manganese – 27.8; Cobalt – 0.27; Selenium – 0.3; Copper – 12 and Iodine – 1.1 and the lowest concentration of Mangan in milk and in the body. The cows of the 2nd and 3rd experimental groups were fed with all the trace elements to the norm (for the 2nd experimental group at the expense of zinc sulfates, manganese, cobalt, copper, selenium, Suplex of Selenium and potassium iodide, for the 3rd instead of zinc sulfates, manganese and mixed cobalt compounds were introduced into their ligand complexes). The concentration of trace elements in these groups in 1 kg of DM was, mg: Zinc – 76; Manganese – 76; Cobalt – 0,97; Copper – 12; Selenium – 0.3 and Iodine – 1.1. The concentration of Zinc, Manganese and Cobalt in 1 kg of DM of fodder was reduced by 20% for cows of the 4th experimental group and by 30% for the 5th experimental group.</span></p><p><span lang="EN-US">The results of the conducted searches indicate that the use in feeding of high-yielding cows of various doses of mixed ligand complexes of Zinc, Manganese, and Cobalt on the use of Suplex of Selenium and sulfate copper and potassium iodide in the first 100- days lactation positively affect the growth of their milk production. Animals of experimental groups dominated cows of analogues of the control group, rations of which were scarce on Zinc, Manganese and Cobalt, by average daily yield of natural milk, respectively, by 4.5; 5.8 and 4.0 kg, or 11.63: 14.99 and 10.34%, and these differences were reliable. While the average daily milk yield of cows of the 2nd experimental group exceeded the average daily yield of the 1st control group by 3.8 kg or by 9.82%. In milk of experimental cows, a single increase in the fat content of 0.01–0.09% was also noted. </span></p><p><span lang="EN-US">Carrying out balance research confirmed the data of milk productivity. Thus, in the body of cows of the 3rd, 4th and 5th experimental groups, more Manganese were deposited, respectively by: 540.7; 531.5, and 394.5 mg compared to control and by 367.1, 357.9, and 220.9 mg – in comparison with the 2nd experimental group; in experimental cows of groups 4 and 5, the manganese was deposited less by 9.2 and 146.2 mg in comparison with the 3rd experimental group. With milk in cows of the 1st control group during the day was allocated 20.8 mg of Manganese, and in the 2nd, 3rd, 4th and 5th experimental groups it was allocated more, respectively by 6.7, 9.8, 15.4, and 10.6 mg. Manganese fecal excretion was less in comparison with the 2nd experimental group, in the cows of the 3rd experimental group it was less by 365.9 mg, in the 4<sup>th</sup> group – by 623.3 mg, and in the 5<sup>th</sup> group – by 691.4 mg.</span></p><p><span lang="EN-US">The use of mixed ligand complexes of Zinc, Manganese and Cobalt enhances the milk productivity of high-yielding cows, reduces their need for these elements, improves their digestion and reduces their excretion with feces.</span></p>


Author(s):  
Айметов ◽  
Ruslan Aymetov

The mineral elements interact not only among themselves, but also with the organic components during complex metabolism process. Identifying the features of the feed nutrient substances relationship allows you to direct the body’s metabolism in the direction of their effectiveness and to obtain the maximum output from the animals. The experimental part of the work was carried out on poultry complex “Agrofirma Zalesnyy” of the Republic of Tatarstan from January to May 2016. The objects of research were female turkey-broilers of “Hybrid Converter” cross, mineral supplement “Tseostimul”, probiotic “Provagen” and symbiotic preparation of a new generation. With this aim, four groups of turkey were formed of 40 heads. The first group was a control one and received a basic diet, adopted by the poultry farm. The second group received the basic diet, added with mineral supplements with a dosage of 30 gramm per 1 kilogramm of feed. Turkeys of III-experimental group was added to the diet of probiotic preparation “Provagen” at a dose of 3 gramm per 1 kilogramm of feed. The turkeys of IV-test group received in addition to the basic diet a symbiotic drug rate of 20 gramm per 1 kilogramm of feed. The main diet was consisted of complete feed. Duration of the experiment was 112 days. By the end of the experiment the body weight of experimental groups exceeded the similar index: in the control group to 1082 gramm (р ≥0,001), in the first test group to 781 gramm (р ≥0,001), in the second test group to 1339 gramm (р ≥0,001), in the third group with addition of symbiotic drugs or to 12.3%, 8.9% and 15.3% respectively. The numbers of leukocytes was decreased from 34.2 109/L to 33.3 109/L in I experimental group, to 33.2 109/L in II and to 34.4 109/L in IV experimental group. The mass of internal organs was significantly higher in females of experimental groups. Thus, the weight of the liver of experimental groups was greater to 12.3%, 8.9% and 15.3%, compared with the control group.


2021 ◽  
Vol 6 (1) ◽  
pp. 64-70
Author(s):  
Andrei Frolkin ◽  
Haidar Valitov ◽  
Aleksandr Varakin ◽  
Valentina Kornilova

The purpose of the research is to increase the effectiveness of the supplement Reasil on the growth of milk-fed calves. For the scientific and economic experience, three groups of calves were formed (control, 1 and 2 experi-enced). Groups of animals of 10 heads were formed according to the principle of analogues. The indicators of live weight, growth and blood of calves were studied after Reasil use in a diet: Reasil HumicVet as a liquid form, and Reasil Humic Health – reduced to powder. In the experiment, animals of a black-and-white breed were used. The Reasil feeding in the diet contributed to an increase in the live weight of milk-fed calves in the 1-st and the 2-nd experimental groups at two months of age by 5.65 and 5.48 kg (or by 7.4 and 7.2%), respectively, compared to the indicator of the control group analogues. The average daily growth of calves of the experimental groups exceeded this indicator of control animals by 96 and 82 g, respectively. The blood of calves of the 1st and 2nd experimental groups, showed that hematoglobin was higher by 15.7 and 11.4%, red blood cells – by 16.5 and 15.1%, and the alkaline reserve – by 5.2%, compared with blood of calves from the control group, which indicates the activation of metabolic processes in the body. The total protein amount in the blood serum of animals from the experimental groups increased by 8.4 and 5.9% respectively, compared to the tests of calves from the control group. The albu-min content of animals of the 1st experimental group tested was higher by 5.3%; from the 2nd experimental group there was no significant difference compared to the control. The number of gamma-globulins in the blood of ani-mals of the experimental groups increased, which indicates an increase in defense reaction of animals. The con-tent of calcium in the blood serum of calves from the experimental groups was higher by 8.3 and 5.9%, phosphorus – by 4.8 and 2.4%, which indicates a more effective use of these mineral elements.


Author(s):  
V. Mykytyuk ◽  
T. Vasilenko ◽  
O. Orischuk ◽  
S. Tsap ◽  
I. Porotikova

The results of studies on the effect of various doses of cobalt with an optimized level of sulfur in rations of Askanian meat-and-wool ewes on digestibility of nutrients of consumed feed and digestibility of the studied mineral elements are presented. It was established that the level of nutrient digestibility of rations was higher in ewes of the experimental groups, which, to the generally accepted norm, were additionally injected with cobalt chloride in the amount of 10 %, which was equal 0.825 mg, 20 % – 0.900 mg, and 30 % – 0.975 mg per head per day . As a result of the conducted research, it was found that the ewes of the second experimental group differed in the highest indices of digestibility of nutrients of the ration, which increased the rate of cobalt by 10 %. The additional introduction of 20 % and 30 % cobalt to the basic ration did not contribute to a further increase in the digestibility factors of nutrients in the body of ewes of the experimental groups. The research has shown that the uptake of cobalt by experimental ewes increased as this element increased in the rations. Thus, 0.31 mg of cobalt was assimilated by the ewes of the second experimental group, which is 0.06 mg or 20.0 % higher compared to the control group; to the third-group of ewes by 0.07 mg or 28.0 % and to the fourth-group ewes by 0,08 mg or 32.0 % compared with analogues of the control group. However, when calculating these indicators in relative terms, it was established that the highest percentage of cobalt absorbed from that received was in the ewes of the second experimental group. Their advantage relative to the control group was 4.60 % (P <0.01), and III and IV experimental groups – 2.11 and 0.19 absolute percent.


Author(s):  
I. I. Dvylyuk ◽  
I. I. Kovalchuk

Experimental data of the lipid composition of the honey bees and bee bread in the spring period under the conditions of feeding sugar syrup with Ag and Cu citrates are presented in the article. The studies were conducted on 3 groups of bee colonies, three bee colonies each. Control group (I) received sugar syrup at a concentration of 1:1 (1000 ml per week), experimental (II) group received under similar conditions with sugar syrup 0.2 mg of Argentum and 0.2 mg of Cuprum in citrate form. The third experimental group was fed with sugar syrup Ag and Cu citrates at doses of 0.5 mg each per 1000 ml of sugar syrup per week. The study was lasted 36 days. Samples of tissues of the whole organism of honey bees in the amount of 30–35 pieces were selected for the study. from each bee colony and bee bread to determine the total lipid content by the Folch method and their separate fractions: phospholipids, mono- and diacylglycerols, free cholesterol, non-esterified fatty acids, triacylglycerols, esterified cholesterol glycol cholesterol using thin layer chromatography using silica gel plates Sorbfil. According to the results of the research, we found intergroup differences of total lipid content in the tissues of whole organism of the honey bees and bee bread of experimental groups in comparison with the control group. Higher levels of phospholipids, esterified cholesterol, and lower levels of free cholesterol, mono- and diacylglycerols and triacylglycerols were found in the tissue of bees II and III of the experimental group. Introduction of Ag and Cu citrates into the components of bee feeding in the bee bread the level of phospholipids (P ˂ 0.01), mono and diacylglycerols (group III), triacylglycerols increased against the decrease of free cholesterol (P ˂ 0.05) and esterified cholesterol (P ˂ 0.01).


2021 ◽  
Vol 83 (2) ◽  
pp. 42-50
Author(s):  
I.I. Kovalсhuk ◽  
◽  
R.S. Fedoruk ◽  
M.Ya. Spivak ◽  
M.M. Romanovyсh ◽  
...  

In modern beekeeping it is important to get safe ecological products, stimulate reproduction and increase the resistance of bees to various diseases as well as to protect them from adverse environmental conditions. Due to this, in recent years there has been a tendency to use biologically active preparations and means of protection of natural origin in beekeeping. They may help to avoid many side effects, because the mechanisms of their biological action are significantly different from synthetic and are based on the physiological activation of the organism’s protective reactions. The aim of the research was to determine the biological activity of Lactobacillus casei IMV B-7280 immunobiotic strain in honey bees and its influence on their life expectancy and the content of certain mineral elements in organism tissues. Methods. The research was conducted on honey bees of the Carpathian breed, 25–45 individuals in each group, formed in gardens from three bee families from the apiary-vivarium of the Institute of Animal Biology of NAAS of Ukraine. To determine the influence of the immunobiotic, a control (І) and three experimental (ІІ, ІІІ, IV) groups were formed. The bees of the experimental groups were fed with sugar syrup together with the immunobiotic strain in various concentrations. The alimentation of bees of control and experimental groups was provided under similar conditions of the laboratory thermostat with microventilation at a temperature of 30.0°C and relative humidity of 74–76% during 12 days of research. During the research period, daily counting of the number of live and dead bees, their motor and forage activity was performed. After feeding, 25 bees were selected from each group and grounded on homogenizer. Got homogenate of tissues was used to search the content of individual trace elements on atomic absorption spectrophotometer SF-115 PC with a computer program for calculating the concentration of elements. Results. The corrective influence of the immunobiotic on the content of Fe, Zn, Co, Cu, Cd, Pb in the tissues of bees and their viability depending on the used concentration has been established. Under the conditions of immunobiotic strain addition to the sugar syrup at a concentration from 1x105 to 1x109cells/ml, an increase in the Cobalt content was observed against the background of a decrease in Cadmium and Lead content in tissue samples of bees of the experimental groups compared to the control. The best dynamics in the number of live bees in gardens was observed under the action of high concentration of immunobiotic strain, with the preservation of 100% of live bees during the first 8 days of immunobiotic use. During the period from 9th to 12th days a decrease in the number of live bees from 88 to 82% was detected. In the control group, the survival of bees in this period was 7.3 and 2.4% respectively. Conclusions. The use of Lactobacillus casei IMV B-7280 immunobiotic strain with sugar syrup for feeding bees provides stimulation of their viability, the development of bee colonies, increases the organism’s resistance and optimizes the content of certain mineral elements in tissues. The expediency of further researches of the biological activity and safety of the immunobiotic in different experimental models using honey bees is substantiated.


2019 ◽  
Vol 10 (2) ◽  
pp. 177-181
Author(s):  
I. Kovalchuk ◽  
I. Dvylyuk ◽  
Y. Lecyk ◽  
I. Dvylyuk ◽  
B. Gutyj

The study was conducted on honey bees of the Carpathian breed on the basis of the apiary of S. Z. Gzhytskyi Lviv National University of Veterinary Medicine and Biotechnologies. The bees were kept in 8-frame beehives with size of the frame of 435 × 300 mm. The study was undertaken in the summer-autumn period. A total of 5 groups of bee families were formed according to the analogue principle, with three families of bees in each group. Control (I) group received sugar syrup in 1 : 1 concentration (1000 mL/group/week). In the experimental (II) group, Ag was added to the syrup in amount of 0.5 mL/g of the sugar syrup; in III experimental group – in similar conditions Ag was added in 1 mg/L amount; IV group received sugar syrup with Cu in 0.5 mg/L amount; V experimental group – Cu in amount of 1 mg/L of sugar syrup. Microelements were added to sugar syrup in the form of citrates obtained using method of nanotechnology, developed by the company Nanomaterials and Nanotechnologies in Kyiv. The period of monitoring lasted 28 days with 7 days interval of preparation. Inter-group and anatomic differences were found in content of certain mineral elements in the tissues of the head, thorax and abdominal sections of the honey bees in conditions of feeding with citrates of argentum and cuprum in sugar syrups. Under the influence of citrate of argentum in amount of 0.5 and 1.0 mg/L of sugar syrup, content of ferrum increased in the tissues of the head and abdominal sections, though it decreased in the thorax, content of cuprum decreased in the tissues of head, thorax and abdomen, content of zinc increased in the tissues of head and thorax, though it decreased in the abdomen, content of lead and cadmium reduced in the tissues of all anatomic sections of the organism of honey bees. Under the influence of cuprum in amount of 0.5 and 1.0 mg/L of sugar syrup, content of ferrum reduced in the tissues of the head, though it increased in the tissues of thorax and abdomen, content of cuprum increased, and the content of zinc, lead and cadmium reduced in the tissues of all anatomic sections of honey bees. According to the results of the study, the determining effect of feeding of honey bees on the optimization of the parameters of mineral metabolism in different anatomic sections of the organism of bees is discussed.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.


Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


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