scholarly journals Effect of the Extraction Solvent Polarity and the Ratio of Feed and Solvent on the Phytochemical Content and Antioxidant Activity of Red Betel Leaves (Piper crocatum)

2018 ◽  
Vol 1 (1) ◽  
pp. 71-77
Author(s):  
Monika Rahardjo ◽  
Gelora Mangalik ◽  
Monang Sihombing ◽  
Junet Franzisca Da Costa
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Solvent Polarity ◽  
Ornamental Plant ◽  
Leaf Sample ◽  
Phytochemical Content ◽  
Extraction Solvent ◽  
Betel Leaf ◽  
Solvent Type ◽  
Tropical Areas

Red betel leaf (Piper crocatum) is a plant growing in tropical areas and previously known as an ornamental plant, but then later used as medicinal plant. Previous researches found that red betel leaves contain bioactive compounds such as alkaloids, flavonoids, tannins, and many more that have potential to be used as antioxidant. The extractions were carried out using variations of solvent types (ethanol, water, and ethyl acetate) and feed to solvent (F:S) ratios in g/ml (F:S=1:20, F:S=1:25, F:S=1:30). The best conditions from this research were the usage of ethanol as the solvent. In addition to its ability to extract the compounds potential as antioxidant and F:S ratio of 1:30, it could give highest yield of extract. Variation of solvent type and variation of F:S have significant effect on the value of antioxidant activity (IC50) of the red betel leaf sample.

PENGARUH JENIS PELARUT TERHADAP RENDEMEN DAN AKTIVITAS ANTIOKSIDAN DALAM EKSTRAK MINYAK BEKATUL PADI (RICE BRAN OIL)

EKUILIBIUM ◽  
2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Agus Purwanto
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Rice Bran ◽  
Rice Bran Oil ◽  
Economic Value ◽  
Solvent Polarity ◽  
Acid Number ◽  
Free Radical Damage ◽  
Solvent Type ◽  
Rice Milling

<p>Abstract: Bran rice is a by-product of rice milling which only used as cattle feed. Utilization of<br />bran rice to take rice bran oil will increase its economic value. Rice bran oil contains natural<br />antioxidants -oryzanol and fatty acids. Antioxidants -oryzanol are more powerful than vitamin<br />E to avoid free radical damage. One way to recovery rice bran oil is extraction using volatile<br />solvents. The effects of solvent type to yield of rice bran oil and antioxidant activity of rice bran<br />oil need to be researched. This study started with the process of stabilization of bran to inhibit<br />lipase activity. Next process was extraction of bran oil using n-hexane, ethyl acetate, and<br />ethanol as the solvent. The next step was evaluation the acid number and antioxidant activity of<br />obtained oil with DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The results showed that the<br />yields of oil were affected by solvent polarity compounds. Highest yield was obtained on<br />extraction using ethanol and the yield was 12.553%, 14.105% and 17.431%. Acid test showed<br />that extraction using ethyl acetate produced oil with smallest acid number (79.662 and 90.882)and test of antioxidant activity showed that extraction with ethanol was potential as antioxidants<br />which value of IC<br />50<br />were 46.79% and 47.29%.<br />Keywords: bran, extraction, solvent type, antioxidants, DPPH</p>

scholarly journals Antioxidant Activity and Phytochemical Content of Clerodendrum serratum L. from Different Provinces of Chhattisgarh State, India

2019 ◽  
Vol 31 (10) ◽  
pp. 2147-2151
Author(s):  
Seema Upadhyay ◽  
Vijaya Koche
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Correlation Study ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Medicinal Value ◽  
Ancient Times ◽  
Over Exploitation ◽  
Important Medicinal Plant ◽  
Body Ache

Clerodendrum serratum L. is one of the important medicinal plant found in Chhattisgarh state of India. Its over-exploitation for medicinal value made it a threatened species according to Chhattisgarh Medicinal Plant Board. It is being used in Ayurveda from ancient times for many diseases like asthma, body ache, bronchitis, cholera, dropsy, eye diseases, fever, inflammations, malaria, ophthalmia, rheumatism, snakebite, tuberculosis, ulcers and wounds. Present study aimed to compare the phytochemical contents and antioxidant activity of a medicinally important plant C. serratum L. collected from different provinces of Chhattisgarh state (India). Plants were collected from three districts of Chhattisgarh (Jagdalpur, Bilaspur and Raipur). Aerial and underground parts were separated and subjected for extraction by using different solvents of different polarity. These extracts were evaluated for phytochemical profiling, phytochemical content (total phenolic and flavonoids content) and antioxidant activity. Methanolic extract shows highest antioxidant activity and phenolic and flavonoids content among all the extracts. When the plants of different regions were compared, it was found that plants of Jagdalpur district is a potent source of phytochemical and showing highest antioxidant activity. Further, the correlation study showed that phenolics and flavonoids mainly account for antioxidant activity. It may be possible that some different groups of phytochemical act synergistically together with the phenolics and flavonoids and provide antioxidant effect.

The Influence of Extraction Solvent on the Active Principles Content of Portulaca Oleracea Native Species

2018 ◽  
Vol 69 (10) ◽  
pp. 2682-2692 ◽  
Author(s):  
Candice Popescu (Popiniuc) ◽  
Carmen Popescu ◽  
Stefan Manea ◽  
Valentin Vladut ◽  
Iulian Voicea ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Propylene Glycol ◽  
Native Species ◽  
Portulaca Oleracea ◽  
Extraction Solvent ◽  
Beneficial Effects ◽  
Solvent Systems

In this paper, a study on the influence of extraction solvent on the active principles content of purslane - Portulaca oleracea extracts was performed. For the study were used both fresh and dried plants, chopped by plant chopping machine. The following extracts were obtained and analysed in the solvent systems mentioned below: (fresh) purslane extract in Ethanol 30%, (dry) purslane extract in Ethanol 30%; (fresh) purslane extract in EtOH 70%, (dry) purslane extract in EtOH 70%; (fresh) purslane extract in propylene glycol 20%, (dry) purslane extract in propylene glycol 20%; (fresh) purslane extract in PG 100%, (dry) purslane extract in PG 100%; (fresh) purslane extract in glycerine 20%, (dry) purslane extract in glycerine 20%; (fresh) purslane extract in R glycerine, (dry) purslane extract in R glycerine; purslane hydro-alcoholic glycerine extract. The results obtained in the study performed on purslane extracts in different solvents show that the capitalization of purslane can become very profitable and with beneficial effects on economy in our country, both in terms of food and as medicinal plant.

scholarly journals Comparative Study on Seed Characteristics, Antioxidant Activity, and Total Phenolic and Flavonoid Contents in Accessions of Sorghum bicolor (L.) Moench

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3964
Author(s):  
Bimal-Kumar Ghimire ◽  
Ji-Won Seo ◽  
Chang-Yeon Yu ◽  
Seung-Hyun Kim ◽  
Ill-Min Chung
Keyword(s):  
Antioxidant Activity ◽  
Agronomic Traits ◽  
Germination Rate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Extensive Study ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Seed Characteristics ◽  
Seed Extracts

Sorghum is a major cereal food worldwide, and is considered a potential source of minerals and bioactive compounds. Its wide adaptive range may cause variations in its agronomic traits, antioxidant properties, and phytochemical content. This extensive study investigated variations in seed characteristics, antioxidant properties, and total phenolic (TPC) and flavonoid contents (TFC) of sorghum collected from different ecological regions of 15 countries. The antioxidant potential of the seed extracts of various sorghum accessions was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging assays. Significant variations in TPC were observed among the sorghum accessions. All 78 sorghum accessions used in this study exhibited significant variations in TFC, with the lowest and highest amount observed in accessions C465 and J542, respectively. DPPH scavenging potential of the seed extracts for all the accessions ranged from 11.91 ± 4.83 to 1343.90 ± 81.02 µg mL−1. The ABTS assay results were similar to those of DPPH but showed some differences in the accessions. Pearson’s correlation analysis revealed a wide variation range in the correlation between antioxidant activity and TPC, as well as TFC, among the sorghum accessions. A wide diversity range was also recorded for the seed characteristics (1000-seed weight and seed germination rate). A dendrogram generated from UPGMA clustering, based on seed traits, antioxidant activity, TPC, and TFC was highly dispersed for these accessions. Variations among the accessions may provide useful information regarding the phytoconstituents, antioxidant properties, and phytochemical contents of sorghum and aid in designing breeding programs to obtain sorghum with improved agronomic traits and bioactive properties.

scholarly journals IDENTIFIKASI SENYAWA FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KASAR DAUN PEPE

2019 ◽  
Vol 8 (2) ◽  
pp. 111
Author(s):  
Dewa Gede Eka Prayoga ◽  
Komang Ayu Nocianitri ◽  
Ni Nyoman Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Data ◽  
Qualitative Data ◽  
Total Phenol ◽  
Total Flavonoids ◽  
Randomized Design ◽  
Solvent Type ◽  
Ic50 Values ◽  
Phytochemical Compounds ◽  
Completely Randomized Design

This research was conducted to identify the phytochemical compounds and antioxidant activity of pepe leaves (Gymnema reticulatum Br.) crude extract in various solvent types. The experimental design used in this research was a completely randomized design with solvent type treatments consisting of 5 types : aquadest, ethyl acetate 96%, acetone 70%, ethanol 70%, and methanol 95%. The treatment was repeated three times, so that 15 experimental units were obtained. Qualitative data obtained in this research were displayed with tables, while quantitative data were analyzed by variance and if the treatment had a significant effect, then followed by Duncan's test. The results showed that the treatment of solvent types affected phytochemical compounds that was extracted from pepe leaves and had a significant effect (P<0.01) on yield, total phenol, total flavonoids, and antioxidant activity. The best results showed that 70% acetone solvents were able to extract phytochemical compounds from alkaloids, saponins, phenols, flavonoids, and steroids in the form of sterols and had the highest antioxidant activity based on 62.06% free radical inhibition percentage with IC50 values of 200.775 mg/L, yield 32.35%, total phenol 36.14 mgGAE/g extract, and total flavonoids 50.37 mgQE/g extract.

Antioxidant and anticancer activities of an Aporphine alkaloid isolated from Alphonsea sclerocarp

2018 ◽  
Vol 7 (1) ◽  
pp. 51-55
Author(s):  
Suman Joshi DSD ◽  
◽  
Chander Amgoth ◽  
Surya Narayana S ◽  
Padmavathi CH ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Chronic Myeloid Leukaemia ◽  
Mtt Assay ◽  
Preparative Hplc ◽  
Spectroscopic Techniques ◽  
Anticancer Activities ◽  
Frap Assay ◽  
Aporphine Alkaloid ◽  
Dose Dependent

Herein, we report on the anticancer and antioxidant activity of an Aporphine alkaloid isolated from medicinal plant Alphonsea sclerocarpa. Conventional column chromatography and preparative HPLC methods were used to isolate and purify the alkaloid. Based on NMR, FTIR and MS/MS spectroscopic techniques the compound was identified as Crebanine. This is the first report on the presence of Crebanine from the leaves of Alphonsea sclerocarpa. Antioxidant activity was evaluated using DPPH and FRAP assay. The antioxidant activity was dose-dependent and activity increased with the increase in the concentration. The cytotoxicity assay was carried out using MTT assay. The results showed remarkable cytotoxic activity against K562 (CML-chronic myeloid Leukaemia blood cancer) cells with a % inhibition of 37.545 at 500µm concentration after an incubation of 24h

Sign in / Sign up

Export Citation Format

Share Document