scholarly journals Antioxidant Activity and Phytochemical Content of Clerodendrum serratum L. from Different Provinces of Chhattisgarh State, India

2019 ◽  
Vol 31 (10) ◽  
pp. 2147-2151
Author(s):  
Seema Upadhyay ◽  
Vijaya Koche
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Correlation Study ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Medicinal Value ◽  
Ancient Times ◽  
Over Exploitation ◽  
Important Medicinal Plant ◽  
Body Ache

Clerodendrum serratum L. is one of the important medicinal plant found in Chhattisgarh state of India. Its over-exploitation for medicinal value made it a threatened species according to Chhattisgarh Medicinal Plant Board. It is being used in Ayurveda from ancient times for many diseases like asthma, body ache, bronchitis, cholera, dropsy, eye diseases, fever, inflammations, malaria, ophthalmia, rheumatism, snakebite, tuberculosis, ulcers and wounds. Present study aimed to compare the phytochemical contents and antioxidant activity of a medicinally important plant C. serratum L. collected from different provinces of Chhattisgarh state (India). Plants were collected from three districts of Chhattisgarh (Jagdalpur, Bilaspur and Raipur). Aerial and underground parts were separated and subjected for extraction by using different solvents of different polarity. These extracts were evaluated for phytochemical profiling, phytochemical content (total phenolic and flavonoids content) and antioxidant activity. Methanolic extract shows highest antioxidant activity and phenolic and flavonoids content among all the extracts. When the plants of different regions were compared, it was found that plants of Jagdalpur district is a potent source of phytochemical and showing highest antioxidant activity. Further, the correlation study showed that phenolics and flavonoids mainly account for antioxidant activity. It may be possible that some different groups of phytochemical act synergistically together with the phenolics and flavonoids and provide antioxidant effect.

scholarly journals Comparative Study on Seed Characteristics, Antioxidant Activity, and Total Phenolic and Flavonoid Contents in Accessions of Sorghum bicolor (L.) Moench

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3964
Author(s):  
Bimal-Kumar Ghimire ◽  
Ji-Won Seo ◽  
Chang-Yeon Yu ◽  
Seung-Hyun Kim ◽  
Ill-Min Chung
Keyword(s):  
Antioxidant Activity ◽  
Agronomic Traits ◽  
Germination Rate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Extensive Study ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Seed Characteristics ◽  
Seed Extracts

Sorghum is a major cereal food worldwide, and is considered a potential source of minerals and bioactive compounds. Its wide adaptive range may cause variations in its agronomic traits, antioxidant properties, and phytochemical content. This extensive study investigated variations in seed characteristics, antioxidant properties, and total phenolic (TPC) and flavonoid contents (TFC) of sorghum collected from different ecological regions of 15 countries. The antioxidant potential of the seed extracts of various sorghum accessions was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging assays. Significant variations in TPC were observed among the sorghum accessions. All 78 sorghum accessions used in this study exhibited significant variations in TFC, with the lowest and highest amount observed in accessions C465 and J542, respectively. DPPH scavenging potential of the seed extracts for all the accessions ranged from 11.91 ± 4.83 to 1343.90 ± 81.02 µg mL−1. The ABTS assay results were similar to those of DPPH but showed some differences in the accessions. Pearson’s correlation analysis revealed a wide variation range in the correlation between antioxidant activity and TPC, as well as TFC, among the sorghum accessions. A wide diversity range was also recorded for the seed characteristics (1000-seed weight and seed germination rate). A dendrogram generated from UPGMA clustering, based on seed traits, antioxidant activity, TPC, and TFC was highly dispersed for these accessions. Variations among the accessions may provide useful information regarding the phytoconstituents, antioxidant properties, and phytochemical contents of sorghum and aid in designing breeding programs to obtain sorghum with improved agronomic traits and bioactive properties.

scholarly journals Effect of the Extraction Solvent Polarity and the Ratio of Feed and Solvent on the Phytochemical Content and Antioxidant Activity of Red Betel Leaves (Piper crocatum)

2018 ◽  
Vol 1 (1) ◽  
pp. 71-77
Author(s):  
Monika Rahardjo ◽  
Gelora Mangalik ◽  
Monang Sihombing ◽  
Junet Franzisca Da Costa
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Solvent Polarity ◽  
Ornamental Plant ◽  
Leaf Sample ◽  
Phytochemical Content ◽  
Extraction Solvent ◽  
Betel Leaf ◽  
Solvent Type ◽  
Tropical Areas

Red betel leaf (Piper crocatum) is a plant growing in tropical areas and previously known as an ornamental plant, but then later used as medicinal plant. Previous researches found that red betel leaves contain bioactive compounds such as alkaloids, flavonoids, tannins, and many more that have potential to be used as antioxidant. The extractions were carried out using variations of solvent types (ethanol, water, and ethyl acetate) and feed to solvent (F:S) ratios in g/ml (F:S=1:20, F:S=1:25, F:S=1:30). The best conditions from this research were the usage of ethanol as the solvent. In addition to its ability to extract the compounds potential as antioxidant and F:S ratio of 1:30, it could give highest yield of extract. Variation of solvent type and variation of F:S have significant effect on the value of antioxidant activity (IC50) of the red betel leaf sample.

scholarly journals Antioxidant Activity and Antibacterial Seaweed Methanol Extract (Sargassum Duplicatum J. Agardh) and Its Potential as a Natural Preservative Alternative to Salted Eggs

2017 ◽  
Vol 2 (1) ◽  
pp. 133
Author(s):  
Satria Bagus Firmansyah ◽  
R. Arizal Firmansyah ◽  
Nur Hayati
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Research Activity ◽  
Natural Preservative ◽  
Phytochemical Content ◽  
Preservation Method ◽  
Dpph Method

<p style="text-align: justify;">The research activity of the antioxidant and antibacterial esktrak methanol seaweed Sargussum duplicatum J. Agardh as well as its potential as an alternative natural preservative salted egg has been done. Seaweed extract S. duplicatum Jj. Agardh assayed phytochemistry and the content of total phenolic with variation of temperature. Metabolite secondary in seawed extract obtained through maseration using methanol solvent. The extract are partitioned, R1 and R2. R1 sample is a sample without treatment and sample R2 is a sample with 45 minutes for warming treatment in waterbath temperature to 100 °C. Phytochemical content in seawed of S. duplicatum J. Agardh are flavonoid dan steroid. Phytochemical content and phenolic total have positive correlation to antioxidant activity. Antioxidant activity with DPPH method yielded IC50 = 143.03 μg/mL (R1) and 357.95 μg/mL (R2). Antibacterial activity assay carried out trough diffusion method with SSA media (Salmonella-Shigella Agar). Inhibition value toward Salmonella is 1.120 mm and 1.15 mm with control is chloramphenicol. Salmonella sp.is pathogenic bacteria that presents in egg and causes decay on the egg. Discussion about antibacteria activity results of seawed extract of S. duplicatum J. Agardh are suggested to apply toward egg preservation method. ©2016 JNSMR UIN Walisongo. All rights reserved</p>

scholarly journals Identification of Bioactive Compounds and Analysis of Inhibitory Potential of the Digestive Enzymes from Syzygium sp. Extracts

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Tuanny Cavatão de Freitas ◽  
Ronaldo Junio de Oliveira ◽  
Ricardo José de Mendonça ◽  
Pamela Aparecida Candido ◽  
Luciana Lopes Silva Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Bioactive Compounds ◽  
Digestive Enzymes ◽  
Simulated Gastric Fluid ◽  
Total Phenolic ◽  
Crystallographic Structure ◽  
Inhibitory Potential ◽  
Diabetes And Obesity

Diabetes and obesity represent major public health problems worldwide. High cost of medicines and drug treatments propose the research for less expensive alternatives, such as enzymatic inhibitors present in medicinal plants from natural sources. An example of such medicinal plant is the jambolan Syzygium sp., which is referred to be hypoglycemic and efficient in weight loss. With this in mind, we identified the bioactive compounds from Syzygium sp. commercial teas and evaluated the inhibitory potential and the antioxidant activity of digestive enzymes from a simulated gastric fluid. Syzygium sp. samples showed low percentages of enzymatic inhibition at 1 : 200 dilution. Antioxidant activity was significant, although it was not expressive of the contents of total phenolic compounds, tannins, flavonoids, flavones, and alkaloids. Maldi-Tof spectroscopy suggested the presence of luteolin in Syzygium sp. samples. Molecular docking predicted that luteolin binds at the α-amylase catalytic site in a similar manner as acarbose, the carbohydrate inhibitor from the enzyme crystallographic structure. The phytochemical content and biological activity were distinct among samples from commercial teas. Thus, additional studies should be conducted to elucidate efficacy and safety of Syzygium sp. extracts, especially in vivo experiments. Syzygium sp. might be in the near future recommended as a medicinal plant in low cost diabetes and obesity treatments.

scholarly journals Improving Polyphenolic Compounds: Antioxidant Activity in Chickpea Sprouts through Elicitation with Hydrogen Peroxide

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1791
Author(s):  
Liliana León-López ◽  
Yudith Escobar-Zúñiga ◽  
Nancy Yareli Salazar-Salas ◽  
Saraid Mora Rochín ◽  
Edith Oliva Cuevas-Rodríguez ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Desirability Function ◽  
Biological Activities ◽  
Bioactive Compound ◽  
Biochanin A ◽  
Total Phenolic ◽  
Promising Alternative ◽  
Phytochemical Content ◽  
Germination Time

Elicitation appears to be a promising alternative to enhance the bioactive compound content and biological activities of legume sprouts. Multi-response optimization by response surface methodology (RSM) with desirability function (DF) was used to optimize the elicitor concentration (hydrogen peroxide (H2O2)) and germination time in order to maximize total phenolic content (TPC), total flavonoids content (TFC), and antioxidant activity (AOX) of chickpea sprouts. Chemical, antinutritional, and nutraceutical properties of optimized chickpea sprouts (OCS) were also determined. The predicted regression models developed were efficiently fitted to the experimental data. The results of the desirability function revealed that optimum attributes in chickpea sprouts can be achieved by the application of 30 mM H2O2 and 72 h of germination time, with global desirability value D = 0.893. These OCS had higher (p < 0.05) TPC (7.4%), total iso-flavonoids (16.5%), AOX (14.8%), and lower phytic acid (16.1%) and saponins (21.8%) compared to H2O2 non-treated chickpea sprouts. Optimized germination conditions slightly modified the flavonoid profile in chickpea; eight iso-flavonoids were identified in OCS, including formononetin and biochanin A, which were identified as the major compounds. Results from this study support elicitation with H2O2 as an effective approach to improve phytochemical content and antioxidant activity in chickpea sprouts.

scholarly journals Freeze Dried Acerola (Malpighia emarginata) Pulp and Pomace: Physicochemical Attributes, Phytochemical Content and Stability during Storage

2017 ◽  
Vol 1 (1) ◽  
pp. 17 ◽  
Author(s):  
Francisca Pereira De Moraes ◽  
Aline Cardoso Gonçalves ◽  
Thaís Bezerra Veríssimo Miguel ◽  
Kátia Cristina Borges ◽  
Roberta T P Correia
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Gum Arabic ◽  
Total Phenolic ◽  
Food Ingredients ◽  
Phytochemical Content ◽  
Freeze Dried ◽  
Malpighia Emarginata ◽  
Bioactive Content ◽  
Monomeric Anthocyanins

In this study, acerola pulp and acerola pomace were freeze dried with and without gum Arabic (GA) and analyzed in regard to their physicochemical attributes, bioactive content, and phytochemical stability (total phenolic content, ascorbic acid, total monomeric anthocyanins and antioxidant activity), during storage at 4 oC and 25 oC. The freeze dried products were highly porous and had low water activity (0.273 to 0.300). Freeze dried acerola pulp with GA were the most soluble samples (64.1% to 73.7 %). The addition of GA to freeze dried acerola pomace increased samples hygroscopicity from 4.5% to 11.1%. High ascorbic acid retention in freeze dried acerola pulp was observed for all samples (65.2 % at 25 °C and 88.9% at 4 °C). It was observed that freeze dried acerola pomace showed increased antioxidant activity during storage (101.9% at 4 °C and 135.9% at 25 °C). Our results demonstrate the potential of freeze dried acerola pulp and pomace as novel food ingredients with concentrated phytochemical content and desirable physicochemical attributes. 

Determination of Supercritical Carbon Dioxide Extraction Parameters for Quercus infectoria Galls and the Effects on Extraction Yield and Antioxidant Activity

2014 ◽  
Vol 67 (2) ◽  
Author(s):  
Liza Md Salleh ◽  
Hasmida Mohd Nasir ◽  
Harisun Yaakob ◽  
Mohd Azizi Che Yunus
Keyword(s):  
Carbon Dioxide ◽  
Antioxidant Activity ◽  
Supercritical Carbon Dioxide ◽  
Medicinal Plant ◽  
Flow Rate ◽  
Total Phenolic ◽  
Extraction Parameters ◽  
Quercus Infectoria ◽  
Supercritical Carbon

Currently, finding alternative ways of extracting medicinal plant gain more interest from the researchers.  Quercus infectoria, a medicinal plant, is rich with bioactive compound being extracted using supercritical carbon dioxide (SC-CO2) extraction which helps to maintain the quality of the product as well as green environment. CO2 is widely used as solvent due to moderate critical conditions, nontoxic and easily removed from the products. This work was performed to determine the optimum extraction parameters of SC-CO2 extraction and their effects on the total phenolic content and antioxidant activity of Q.infectoria extract. Hence, two different parameters have been investigated which were extraction time and CO2 flow rate (2, 3, 4 ml/min) while pressure (P) and temperature (T) were fixed at highest density (P = 30 MPa, T = 40oC). The results obtained from this study show that the solvent flow rate of 2 ml/min gives the highest percentage of yield (0.3652%) and the complete extraction of the sample was achieved at 80 minutes. Better quality of the extract was shown at 2 ml/min as resulted in high amount of phenolic compound in the extract presented as gallic acid equivalent (GAE) (2.04×102 mg GAE/g sample). The extracts were screened for possible antioxidant activity by 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging assays. In this study, the best result obtained for antioxidant activity was at flow rate of 3 ml/min with inhibition percentage of 96.97%.

scholarly journals Ontogenetic Development of Hedysarum alpinum L. in the Prebaikalia Region

2020 ◽  
pp. 35-40
Author(s):  
Elena G. Khudonogova ◽  
Alena A. Mikhlyaeva ◽  
Svetlana V. Polovinkina
Keyword(s):  
Medicinal Plant ◽  
Life Span ◽  
Introduced Species ◽  
Plant Species ◽  
Biomass Production ◽  
Raw Material ◽  
Shoot Biomass ◽  
Aerial Parts ◽  
Medicinal Value ◽  
Important Medicinal Plant

Hedysarum alpinum L. is an important medicinal plant, which also has ornamental value and is used as a non-conventional feed resource. The medicinal value of this plant species is attributable to the content of the glycoside mangiferin in the aerial parts of a plant, which demonstrates immunostimulating properties. Harvesting of H. alpinumas a herbal raw material leads to a fast depletion of natural coenopopulation areas. When cultivated in Prebaikalia, the plant passes through all ontogenetic stages, entering the reproductive phase at year 2-3 of growth. The mid-reproductive stage, which is the most productive for feeding purposes, lasts 6 to 10 years. For introduced species, the life span of a plant growing in the same place ranges between 12 and 20 years. H. alpinumis a promising medicinal and fodder plant, which tolerates dry summer periods, is winter-hardy, and has high shoot biomass production.

scholarly journals Differences in Parts of Purslane Plants (Portulaca grandiflora Hook.) To Total Phenolic and Flavonoid Content and Antioxidant Activity

2020 ◽  
Vol 7 (1) ◽  
pp. 10
Author(s):  
Husnawati ◽  
Ukhradiya Magharaniq Safira Purwanto ◽  
Aulia Ayu Rispriandari
Keyword(s):  
Antioxidant Activity ◽  
The Body ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phytochemical Content ◽  
Plant Parts ◽  
Ic50 Value ◽  
Portulaca Grandiflora ◽  
Flower Organs ◽  
Phenolic And Flavonoid

Antioxidant are needed to prevent free radical inside the body and oxidative stress that promotes the aging process and various degenerative diseases. Portulaca grandiflora Hook. is known to have potential as antioxidant. Differences in plant parts can cause differences in phytochemical content, which can affect its antioxidant activity. This study aims to measure total phenolic and flavonoid content in leaf, old stem, young stem, and flower organs of purslane plant (Portulaca grandiflora Hook.) which is extracted using ethanol 96% and determined the antioxidant activity of the extract with DPPH methode. The result showed that each plant organ of purslane contains different concentration of phenolic and flavonoid compounds. The highest total phenolic and flavonoid content was found in the leaves( 113.26 ± 3.85 mg GAE/g and 97.99 ± 1.28 mg QE/g), but the highest antioxidant activity was found in the old stem with IC50 value 122.15 ± 1.30 ppm (classified as medium antioxidant).   Keywords: Antioxidant, Flavonoid, Phenolic, Portulaca grandiflora Hook.

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