scholarly journals Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) terhadap Struktur Histologis Pankreas Tikus Putih (Rattus norvegicus L.) Hiperglikemia

2021 ◽  
Vol 6 (2) ◽  
pp. 115-123
Author(s):  
Nunuk Shofiati ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Histological Structure ◽  
Distilled Water ◽  
Treatment Groups ◽  
Randomized Design ◽  
Neem Leaves ◽  
Neem Leaf Extract

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok  tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans. 

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

scholarly journals 16. The Number Of Leukocyte And Leukocyte Differential In Broilers That Infected With Eimeria tenella And Given Neem Leaf Extract And Jaloh Extract

2017 ◽  
Vol 11 (2) ◽  
pp. 93-99
Author(s):  
Rizki Aulia ◽  
Sugito Sugito ◽  
M. Hasan ◽  
T. Fadrial Karmil ◽  
Gholib Gholib ◽  
...  
Keyword(s):  
Drinking Water ◽  
Leaf Extract ◽  
Calculated Data ◽  
Positive Control ◽  
Negative Control ◽  
Eimeria Tenella ◽  
Neem Extract ◽  
Randomized Design ◽  
Brachial Vein ◽  
Neem Leaf Extract

The aim of this research was to determine the effect of giving neem leaf extract combined with jaloh towards the total of leukocytes and differential leukocyte in broilers that was infected with Eimeria tenella. This research samples were 20 Cobb stain broilers 14 days old. Design the research was complete randomized design with five treatments and each treatment consists of four repetitions. First treatment (P1) as negative control which was only given mineral water; second treatment (P2) as positive control was is given 5 mg/L anti-stress commercial in drinking water; Third treatment (P3) was given 1000 mg/L Jaloh extract; Fourth treatment (P4)was given 250mg/L neem extract; and fifth treatment (P5)was given 1000mg/L jaloh extract combined with 250mg/L neem extract. Anti-stress commercial and extract treatment dissolved in drinking water. The treatment start from 08.00 until 18.00 for ten days. Next in the 11th day (chicken 25 days old)was inoculated Eimeria tanella sporulatif as much as 1 x 104 ookista/ml orally. Then the blood was taken sampling when the chicken 30 days old (five days after infected). Blood sampling was done in the brachial vein. Furthermore, the number of leukocytes and differential leukocyte.Was calculated data were analyzed using complete randomized design with the help of SPSS for Windows 1.8 program. The result of this research showed that giving jaloh and neem leaf extract were not giving significant effect (P0,05) towards the number of leukocyte as well as differential leukocyte of broilers that was infected with Eimeria tenella. The conclusion of this research were giving jaloh leaf extract and neem for 10 days was not giving significant effect towards number of leukocytes and differential leukocyte broilers that infected with Eimeria tenella

scholarly journals Protective Effect of Aqueous Crude Extract of Neem (Azadirachta indica) Leaves onPlasmodium berghei-Induced Renal Damage in Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Sukanya Chachiyo ◽  
Ubonwan Jaihan ◽  
Somrudee Nakinchat
Keyword(s):  
Protective Effect ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Renal Injury ◽  
Leaf Extract ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Potential Candidate ◽  
Neem Leaves ◽  
Neem Leaf Extract

Malaria is a major public health problem in the world because it can cause of death in patients. Malaria-associated renal injury is associated with 45% of mortality in adult patients hospitalized with severe form of the disease. Therefore, new plant extracts to protect against renal injury induced by malaria infection are urgently needed. In this study, we investigated the protective effect of aqueous crude extract ofAzadirachta indica(neem) leaves on renal injury induced byPlasmodium bergheiANKA infection in mice. ICR mice were injected intraperitoneally with 1 × 107parasitized erythrocytes of PbANKA, and neem extracts (500, 1,000, and 2,000 mg/kg) were given orally for 4 consecutive days. Plasma blood urea nitrogen (BUN) and creatinine levels were subsequently measured. Malaria-induced renal injury was evidenced as marked increases of BUN and creatinine levels. However, the oral administration of neem leaf extract to PbANKA infected mice for 4 days brought back BUN and creatinine levels to near normalcy, and the highest activity was observed at doses of 1,000 and 2,000 mg/kg. Additionally, no toxic effects were found in normal mice treated with this extract. Hence, neem leaf extract can be considered a potential candidate for protection against renal injury induced by malaria.

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals Uji Efektivitas Ekstrak Etanol Daun Angsana (Pterocarpus Indicus Willd) sebagai Biolarvasida terhadap Larva Nyamuk Culex Sp.

2021 ◽  
Vol 1 (1) ◽  
pp. 33-37
Author(s):  
Mohammad Ihsan Abdurrozak ◽  
Livia Syafnir ◽  
Esti Rachmawati Sadiyah
Keyword(s):  
Human Health ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Mosquito Larvae ◽  
Treatment Groups ◽  
Lc50 Value ◽  
Probit Method ◽  
Culex Sp

Abstract. Mosquitoes are often associated with health problems because mosquito bites not only cause itching but some species can also transmit various types of parasites that are harmful to human health. One of them is Culex sp mosquito which is a class of infectious insects (vectors). This study aimed to test the activity of compounds in angsana leaf extract (Pterocarpus indicus Willd) as biolarvasides on Culex sp. mosquitoes and determine the concentration of LC50 value needed. Extraction was carried out by maceration method using 70% ethanol solvent. The extract collected was then tested biolarvaside activity on Culex sp. The study subjects were divided into 8 treatment groups, namely aquades (negative control), 0.05%, 0.1%, 0.2%, 0.5%, 1%, 2% and ABATE 0.1% (positive control). Each group contained 20 mosquito larvae with three replications (triplo). Observations were made for 24 hours at intervals of 1 hour, 4 hours, 8 hours, 12 hours and 24 hours. The biolarvaside activity of angsana leaf extract was analyzed using the probit method. Based on the results of the Probit analysis, the LC50 value was 0.83%. These results indicate that the angsana leaf extract (Pterocarpus indicus Willd) was effective as a biolarvaside. Abstrak. Nyamuk sering dikaitkan dengan masalah kesehatan karena gigitan nyamuk tidak hanya menimbulkan gatal saja tetapi beberapa spesies nyamuk juga dapat menularkan berbagai jenis parasit yang berbahaya bagi kesehatan manusia. Salah satunya yaitu nyamuk Culex sp yang merupakan golongan serangga penular (vektor). Penelitian ini bertujuan untuk pengujian aktivitas senyawa dalam ekstrak daun angsana (Pterocarpus indicus Willd) sebagai biolarvasida pada nyamuk Culex sp dan penentuan nilai LC50. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, Ekstrak yang diperoleh kemudian diuji aktivitas biolarvasida pada nyamuk Culex sp. Subjek penelitian dibagi menjadi 8 kelompok perlakuan, yaitu akuades (kontrol negatif), 0,05%, 0,1%, 0,2%, 0,5%, 1%, 2% dan ABATE 0,1% (kontrol positif).Setiap kelompok berisi 20 ekor larva nyamuk dengan tiga kali pengulangan (triplo) . Pengamatan dilakukan selama 24 jam dengan interval 1jam, 4jam, 8jam, 12jam dan 24jam. Aktivitas biolarvasida ekstrak daun angsana dianalisis dengan menggunakan metode probit. Berdasarkan hasil analisis Probit didapatkan nilai LC50 berada pada konsentrasi 0,83%.Hasil tersebut menunjukkan bahwa Ekstrak daun angsana (Pterocarpus indicus Willd) efektif sebagai biolarvasida.

scholarly journals Uji Efektivitas Ekstrak Paku Pedang (Nephrolepis exaltata) Terhadap Pertumbuhan Jamur Candida albicans

2020 ◽  
Vol 13 (01) ◽  
pp. 33-38
Author(s):  
Diana Mey Merlina ◽  
Ngadiani
Keyword(s):  
Candida Albicans ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Randomized Design ◽  
Experimental Type ◽  
Spss Software ◽  
Negative Controls ◽  
Positive Controls

Plant swords (Nephrolepis exaltata) are ferns in the Lomariopsidaceae tribe which are easily found on the banks of rivers, cliffs, and contain flavonoids and alkaloids that can inhibit the growth of albicans candida fungi. This study aims to determine the effectiveness of Sword nail extract (Nephrolepis exaltata) on the growth of albicans candida fungi. This research is a quantitative laboratory experimental type using a completely randomized design (RAL) with 4 different treatment groups, 1 negative control (pz sterile) and 1 positive control (ketocenazole). The parameters observed were the number of colonies (CFU) and the average area of ​​the inhibition zone (mm). The data was analyzed using SPSS software using one way ANOVA analysis which was then followed by a test using DMRT. The results showed that all concentrations differed from the negative controls, while the extract concentration of 1000 ppm compared to the positive controls had no difference. Sword nail extract (Nephrolepis exaltata) has an influence on the growth of candida albicans mushrooms, namely the number of colonies of 6.78 CFU (6x106) at a concentration of 1000 ppm and inhibition zone 33.10 mm at a concentration of 1000 ppm.   Keywords : Candida albicans, Sword spikes (Nephrolepis exaltata), Inhibitory Zone, Colony Count.

scholarly journals 18. POTENSI EKSTRAK AIR DAUN SERNAI (Wedelia biflora) SEBAGAI ANTINYERI PADA MENCIT (Mus musculus) The Potency of Wedelia biflora Leaf Water Extract as Pain killer on Mice (Mus musculus)

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Abdul Hayi Amarta Ali
Keyword(s):  
Mus Musculus ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Hot Plate ◽  
Plate Method ◽  
Treatment Groups ◽  
Randomized Design ◽  
Group 2 ◽  
Analgesic Potency

The purpose of this research was to find out the potency of Wedelia biflora water extract as pain killer. Completely randomized design (CDR) was used in this study. A total of 30 mice (Mus musculus) were divided into five treatment groups. Mice in group 1 (P0) were administered with mefenamat (positive control) and mice group 2 (P1) were only given aquadest (negative control). Mice in another3 groups (P2, P3, and P4) were administered with Wedelia biflora water extract with the concentration of 100, 75, and 50%, respectively. The painful response time was done using hot plate method and observed at 0, 30, 60, and 90 minutes, whereas abdominal writhing method was applied to count the total of writhing. Data were analyzed using ANOVA followed by Duncan. Result showed that Wedelia biflora water extract with various levels of concentration were affect significantly (P0.05) compared to negative control but no significant different (P0.05) with positive control. The analgesic potency showed significant effect (P0.05) at concentration of 100 and 75% compared to negative control, but no significant different (P0.05) compare to positive control on both hot plate methods and abdominal writhing method, whereas 50% concentration showed analgesic potency (P0.05) only on hot plate method. In conclusion, Wedelia biflora water extract is potential as analgesic on mouse and shows no significant different with mefenamat. ____________________________________________________________________________________________________________________ Key words: analgesik, Wedelia biflora, painful, total of writhe

scholarly journals Dietary supplementation of Neem (Azadirachta indica) leaf extracts improved growth performance and reduced production cost in broilers

2020 ◽  
Vol 13 (6) ◽  
pp. 1050-1055
Author(s):  
Torun Kumar Paul ◽  
Md. Mehedi Hasan ◽  
Md. Anowarul Haque ◽  
Sudipta Talukder ◽  
Yousuf Ali Sarker ◽  
...  
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Leaf Extracts ◽  
Neem Leaves

Background and Aim: Global trend to remove the antibiotic growth promoter (AGP) from animals contributes to the exploration of successful measures to sustain production and reduces the intestinal diseases in the post-AGP era. Plant extracts, therefore, have been used to improve performance and intestinal health. Here, we conducted a study to evaluate the effects of neem (Azadirachta indica) leaf extracts (NLE) as alternatives to AGPs in broiler chickens. Materials and Methods: Sixty day-old broiler chicks were assigned to 12-floor pens, each stocked with five birds and divided into three treatment groups of four pens per treatment. The groups were: Negative control, basal diet without additives; positive control, basal diet with antibiotics and vitamins; and NLE treated group, basal diet supplemented with 0.1% aqua extract of neem leaves. Results: Overall feed intake was significantly (p≤0.05) highest in the positive control. Higher body weight, higher dressing percentage, and lower feed conversion ratio were observed in birds treated with NLE compared to the negative control group (p≤0.05) but not the positive control group (p>0.05). There was no significant variation in hematology between different groups. Furthermore, the economic evaluation indicated that the NLE treatment was found cheaper than control and antibiotic treatment in cost-benefit analysis. Conclusion: We suggest NLE might be a cheaper alternative to antibiotics in broiler production as indicated by improved body weight and feed efficiency.

EFEK EKSTRAK ETANOL DAUN SINGKONG (Manihot Utilissima Pohl) SEBAGAI OBAT ALTERNATIF ANTI REMATIK TERHADAP RASA SAKIT PADA MENCIT

2018 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
Setya Enti Rikomah ◽  
Elmitra Elmitra ◽  
Diana Gustina Yunita
Keyword(s):  
Experimental Study ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Control Test ◽  
Leaf Extracts ◽  
Excitatory Response ◽  
Cassava Leaves

Medicinal plants used for generations one of which is crop cassava leaves. Cassava leaves is believed to cure rheumatism, gout, anemia, constipation, and to increase endurance. This study aims to determine the Effects Of Ethanol Leaf Extract Singkong (Manihot Utilissima Pohl) As Drug Alternatif Antirheumatic Of Pain On Mice. This study was an experimental study using 25 mice. These animals were divided into five groups where each group consisted of 5 mice. Cassava leaf extracts as a control test, flamar gel as a positive control, and distilled water as a negative control. Observations were made for 45 minutes using a hot excitatory response in the form of a decrease in a lick or jump after the treatments. The results showed a decline in the response of mice to lick the feet or jump to the heat stimuli were administered after administration of cassava leaf extract. Cassava leaf extract has an effect antirheumatic to pain in mice. Based on the analysis of SPSS best dose in this study is the first dose (0.65mg / 20gr mice BB).

scholarly journals TOPICAL APPLICATION OF Capsicum frutescens LEAF EXTRACT AS HAIR TONIC IN MICE (Mus musculus)

2020 ◽  
Vol 14 (4) ◽  
pp. 90-92
Author(s):  
Safrida Safrida
Keyword(s):  
Quantitative Research ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Optimum Concentration ◽  
Capsicum Frutescens ◽  
Hair Length ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hair Extract

The research aims to determine the effect of giving cayenne leaf extract in increasing the length and amount of hair on mice. This research belongs to the type of quantitative research. This research used a Completely Randomized Design (CRD) in consisting of 5 treatment and 4 repetitions. The treatments consisted of NC (negative control, not treated), PC (positive control, given a commercial hair extract), P1 (cayenne leaf extract 10%), P2 (cayenne leaf extract 20%), and P3 (cayenne leaf extract 30%). Research parameters included the increase of the hair length and amount of hairs growing in mice. The result showed that giving extract of cayenne leaf significantly influences the length and amount of hairs in mice, with 10% concentration (P2) was the optimum concentration of the extract that can trigger the hair growth. It can be concluded that given extract of cayenne leaf can increase the hairs growth in mice and has potential as hair tonic.

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