scholarly journals Efecto anticonceptivo y postcoital del extracto etanólico de lashojas del Desmodium molliculum (HBK).DC “Manayupa” en ratas hembras Holtzmann

2019 ◽  
pp. 33-41
Keyword(s):  
Statistical Tests ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Alcoholic Solution ◽  
Female Rats ◽  
Male Rats ◽  
Experimental Conditions ◽  
Contraceptive Effect ◽  
Postcoital Contraception ◽  
Group 2

Efecto anticonceptivo y postcoital del extracto etanólico de lashojas del Desmodium molliculum (HBK).DC “Manayupa” en ratas hembras Holtzmann Postcoital contraceptive effect of ethanolic extract of the leaves of Desmodium molliculum (HBK).DC "Manayupa" in Holtzmann female rats Fidel Ernesto Acaro Chuquicaña Facultad de Medicina, Universidad Nacional Mayor de San Marcos DOI: https://doi.org/10.33017/RevECIPeru2012.0018/ Resumen Nuestro objetivo fue determinar el efecto anticonceptivo y postcoital del extracto etanólico de las hojas de Desmodium molliculum (HBK).DC en ratas hembras adultas Holtzmann. Las hojas fueron recolectadas en el valle de Baños del Inca, departamento de Cajamarca, la cual es utilizada como diurética, depurativa de la sangre, antihemorrágica, antiinflamatoria de las vías urinarias, hígado y riñones. Se utilizaron 80 ratas hembras fueron seleccionadas aleatoriamente de acuerdo a los criterios de inclusión, y divididas en 2 grandes grupos, cada uno conformada por 5 subgrupos de 8 ratas hembras y ratas machos para el emparejamiento (1 macho: 2 hembras). Grupo 1: 40 ratas para la evaluación del efecto anticonceptivo del extracto de Desmodium molliculum a una solución 100mg/ml vía oral en dosis 200mg/kg, 600mg/kg y 1000mg/kg. Se utilizaron dos grupos como controles suero fisiológico y Medroxiprogesterona en dosis 15mg/kg; Grupo 2: 40 ratas para la evaluación del efecto postcoital del extracto Desmodium molliculum a una solución de 100mg/ml vía oral a dosis 200mg/kg, 600mg/kg y 1000mg/kg. Se utilizaron dos grupos control suero fisiológico y Levonorgestrel a dosis de 50ug/kg. El efecto anticonceptivo se evaluó mediante la cuantificación de los indicadores gravidez, número de implantaciones y número de fetos; el efecto post-coital se evaluó los indicadores gravidez, número de implantaciones, número de fetos vivos y número de fetos muertos. Del escreening fitoquímico se observa que las muestras en estudio, presentan una considerable cantidad de metabolitos secundarios, destacándose cantidades apreciables de flavonoides, compuestos fenólicos, esteroides, alcaloides y taninos estuvieron en mayor cantidad en el extracto etanólico. De las evaluaciones las ratas que recibieron la solución alcohólica a dosis de 200mg/kg.600mg/kg,1000mg/kg disminuyeron la gravidez, el número fetos e implantaciones en la actividad anticonceptiva y poscoital. Se realizaron análisis descriptivos y pruebas estadísticas de significancia ANOVA y Tukey (p<0.05).Concluyendo que en condiciones experimentales los resultados encontrados demuestran que el extracto etanólico de Desmodium molliculum tiene efecto anticonceptivo y postcoital en ratas hembras Holtzmann. Descriptores: Anticonceptivo, Postcoital, Desmodium molliculum, Plantas Anticonceptiva. Abstract Our objective was to determine the effect of postcoital contraception and ethanol extract of the leaves of Desmodium molliculum (HBK). DC Holtzmann in adult female rats. The leaves were collected in the valley of Baños del Inca, Cajamarca department, which is used as a diuretic, purifying the blood, prevent bleeding, inflammatory urinary tract, liver and kidneys. We used 80 female rats were randomly selected according to the inclusion criteria, and divided into 2 groups, each consisting of 5 subgroups of 8 male rats and female rats for pairing (1 male: 2 females). Group 1: 40 rats to evaluate the contraceptive effect of the extract of Desmodium molliculum a 100mg/ml solution orally at 200mg/kg, 600mg/kg and 1000mg/kg. Two groups as saline controls and 15mg/kg dose medroxyprogesterone. Group 2: 40 rats to evaluate the effect of post-coital Desmodium molliculum extract a 100mg/ml solution orally at 200mg/kg dose, 600 mg / kg and 1000mg/kg. Two control groups were used and Levonorgestrel saline at doses of 50ug/kg. The contraceptive effect was assessed by quantifying indicators pregnancy, number of locations and number of fetuses; the effect was evaluated post-coital pregnancy indicators, number of implantations, number of live fetuses and dead fetuses number. Phytochemical screening shows that the samples under study show a considerable amount of secondary metabolites, highlighting significant amounts of flavonoids, phenolic compounds, steroids, alkaloids and tannins were in greater amounts in the ethanol extract. Evaluation of rats receiving a dose of alcoholic solution 200mg/kg.600mg/kg, 1000mg/kg decreased pregnancy, fetuses and the number deployments and postcoital contraceptive activity. Descriptive analyzes were performed and statistical tests of significance ANOVA and Tukey (p <0.05). Concluding that under experimental conditions the results show that the ethanol extract of Desmodium molliculum effect and postcoital contraception in female rats Holtzmann. Keywords: Anticonceptivo, Postcoital, Desmodium molliculum, Plantas Anticonceptiva.

scholarly journals Moringa oleifera Extract Extenuates Echis ocellatus Venom-Induced Toxicities, Histopathological Impairments and Inflammation via Enhancement of Nrf2 Expression in Rats

2021 ◽  
Vol 28 (1) ◽  
pp. 98-115
Author(s):  
Akindele O. Adeyi ◽  
Sodiq O. Adeyemi ◽  
Enoh-Obong P. Effiong ◽  
Babafemi S. Ajisebiola ◽  
Olubisi E. Adeyi ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Male Rats ◽  
Blood Electrolytes ◽  
Tnf Α ◽  
Group 3 ◽  
Group 2 ◽  
Haematological Indices ◽  
Nrf2 Expression ◽  
Potential Remedy

Echis ocellatus snakebite causes more fatalities than all other African snake species combined. Moringa oleifera reportedly possesses an antivenom property. Therefore, we evaluated the effectiveness of M. oleifera ethanol extract (MOE) against E. ocellatus venom (EOV) toxicities. Thirty male rats were grouped as follows (n = 5): Group 1 (normal control received saline), groups 2 to 6 were administered intraperitoneally, 0.22 mg/kg (LD50) of EOV. Group 2 was left untreated while group 3 to 6 were treated post-envenoming with 0.2 mL of polyvalent antivenom, 200, 400, and 600 mg/kg of MOE respectively. MOE significantly (p<0.05) normalized the altered haematological indices and blood electrolytes profiles. MOE attenuated venom-induced cellular dysfunctions, characterized by a significant increase in NRF2, and concomitant downregulation of increased antioxidant enzymes (SOD and CAT) activities in the serum and heart of the treated rats. MOE normalized the elevated TNF-α and IL-1β in serum and heart tissues. Furthermore, the IgG titre value was significantly (p<0.5) higher in the envenomed untreated group compared to the MOE-treated groups. Hemorrhagic, hemolytic and coagulant activities of the venom were strongly inhibited by the MOE dose, dependently. Lesions noticed on tissues of vital organs of untreated rats were abolished by MOE. Our findings substantiate the effectiveness of MOE as a potential remedy against EOV toxicities.

scholarly journals SUBACUTE TOXICITY TEST OF Rhizophora apiculata BARK EXTRACT ON LIVER AND PANCREAS HISTOPATHOLOGY OF RATS

2021 ◽  
Vol 3 (2) ◽  
pp. 89-97
Author(s):  
Syazili Mustofa ◽  
Isnamurti Ciptaningrum ◽  
Caesaria Sinta Zuya
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Male Rats ◽  
Bark Extract ◽  
Healthy Male ◽  
Subchronic Toxicity ◽  
Mangrove Plant ◽  
Subacute Toxicity ◽  
Rhizophora Apiculata ◽  
Liver And Pancreas

Background: Rhizophora apiculata, one of the mangrove plant widely spread in Indonesia, can be developed as a medicinal plant. The extract of the bark has been found to have antioxidant and anti-inflammatory. However, the toxicity of Rhizophora apiculata has not been established yet.Objective: This research aims to evaluate the toxicity of ethanolic extract of Rhizophora apiculata bark on histopathological changes in rat’s liver and pancreas.Methods: Subacute toxicity study of the ethanol extract of Rhizophora apiculata bark was performed in healthy male rats by administering the extract at doses of 57, 114, 228, 456, and 918 mg/kg of body weight daily for 28 days. The subacute toxicity in rats was determined by histological analyses.Results: No significant adverse effect of the extract at dose 57 mg/kg was found. However at and over 114 mg/kg dose of the extract exhibited toxicities to the rats’ liver. In addition, the toxic effect appeared in rats’ pancreas at and over 228 mg/kg dose.Conclusions: Rhizophora apiculata bark extract showed no toxicity at or below 57 mg/kg. The ethanol extract from bark of Rhizophora apiculata showed toxicity at 114 mg/kg by subchronic toxicity.

scholarly journals Difference of Effectiveness Acarbose with Ethanol Extract Purple Eggplant’s Peel (Solanum melongena L) to Reduce Blood Sugar Levels in White Rat (Rattus norvegicus) Induced by Sucrose

2017 ◽  
Vol 6 (2) ◽  
pp. 14
Author(s):  
Nugroho Eko Wirawan Budianto ◽  
Hairullah Hairullah
Keyword(s):  
Blood Sugar ◽  
Solanum Melongena ◽  
Ethanol Extract ◽  
Male Rats ◽  
Flavonoid Compound ◽  
White Rats ◽  
Significant Difference ◽  
Oral Sucrose ◽  
Group 2 ◽  
Sugar Levels

Acarbose is an oligosaccharide human made who is a competitive inhibitor acarbose and can inhibit the action of the enzyme α-glucosidase, causing a slowdown in the digestion of carbohydrate compounds. Purple eggplant skin is included in the flavonoid compound. Purple eggplant  skin as well as an antioxidant that can control blood glucose levels and prevent complications of diabetes. Purple eggplant skin contains flavonoid kaempferol and has the effect of inhibiting the enzyme alpha-glucosidase. Based on these researchers want to know the difference in effectiveness between the ethanol extract of purple eggplant skin  and acarbose to decrease blood sugar levels of mice. The study population using white rats with a total sample size of 30 rats. Research data analysis using One Way Anova Test. Methods: Male rats strain Wistar were divided into 5 groups. 1) Control (CMC Na 1 ml io), 2) Group 1  (The ethanol extract of 50 mg / kg in the CMC Na io),  3) Group 2  (The ethanol extract 100 mg / kg in CMC Na io), 4) Group 3  (the ethanol extract 200 mg / kg in the CMC Na io), 5) Group 4 (Acarbose 4.5 mg / kg in distillated water io). The result showed ethanol extract of purple eggplant skin in group 2 showed the best effectiveness in lowering blood sugar levels of white rats by oral sucrose injection. And there is no significant difference between the effectiveness of the ethanol extract of purple eggplant skin and acarbose in lowering blood sugar levels of white rats by oral sucrose injection.

scholarly journals Ganoderma Lucidum from Red Mushroom Attenuates Formaldehyde-Induced Liver Damage in Experimental Male Rat Model

Biology ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 313
Author(s):  
Babatunde Oluwafemi Adetuyi ◽  
Tolulope Olamide Okeowo ◽  
Oluwatosin Adefunke Adetuyi ◽  
Oluwaseun Abraham Adebisi ◽  
Olubanke Olujoke Ogunlana ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Male Rats ◽  
Male Rat ◽  
Protective Potential ◽  
Group 4 ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2 ◽  
Mediated Reduction

The majority of liver-related illnesses are caused by occupational and domestic exposure to toxic chemicals like formaldehyde (FA), which is widely common in Africa and the world at large. Hence, measures should be taken to protect humans from its hazardous effects. This study, therefore, examines the protective potential of Ganoderma lucidum (100 mg/kg body weight) on formaldehyde-induced (40%) liver oxido-inflammation in male rats. Male Wistar rats, 150–200 g, were allotted into four groups of 10 animals as follows: Group 1 was orally treated with 1 mg/mL distilled water, Group 2 was exposed to a 40% formaldehyde vapor environment for 30 min per day, Group 3 was orally treated with 100 mg/kg ethanol extract of Ganoderma lucidum, and Group 4 was co-administered formaldehyde and 100 mg/kg ethanol extract of Ganoderma lucidum. Rats were then sacrificed 24 h after administering the last dose of treatment, and the livers were excised. Ganoderma lucidum significantly reversed the formaldehyde-mediated reduction in body and organ weight. Ganoderma lucidum administration significantly prevented oxido-inflammation by reducing the levels of hydrogen peroxide and malondialdehyde and increasing the activity of antioxidant enzymes and glutathione contents, as well as the normal level of nitrite and myeloperoxidase production in FA-treated rats. Additionally, Ganoderma lucidum reversed a large decline in proinflammatory markers in formaldehyde. Furthermore, Ganoderma lucidum restores formaldehyde-induced histological alterations in the liver. Collectively, our results provide valuable information on the protective potential of Ganoderma lucidum in protecting formaldehyde-induced liver oxido-inflammation in male rats.

scholarly journals MICROBODIES IN EXPERIMENTALLY ALTERED CELLS

1967 ◽  
Vol 35 (1) ◽  
pp. 127-152 ◽  
Author(s):  
Donald Svoboda ◽  
Harold Grady ◽  
Daniel Azarnoff
Keyword(s):  
Female Rats ◽  
Male Rats ◽  
Hypolipidemic Effect ◽  
Kidney Cells ◽  
Experimental Conditions ◽  
Hypolipidemic Drug ◽  
Sufficient Cause ◽  
Monkey Liver ◽  
Liver And Kidney ◽  
Sustained Increase

A rapid and sustained increase in the number of microbodies in liver and kidney cells can be induced in male rats by ethyl chlorophenoxyisobutyrate (CPIB), a hypolipidemic drug. This phenomenon permits investigation of several aspects of microbody behavior in experimental conditions. Reversal experiments demonstrate that liver cells revert to normal between 2 and 3 weeks after withdrawal of CPIB and that one of the mechanisms for removal of excess microbodies is their incorporation into structures indistinguishable from lysosomes. In a state of rapid cell division, such as that present during liver regeneration, microbody proliferation apparently occupies a high biological priority. In necrotic or degenerating cells microbody structure remains relatively normal. The increase in microbodies induced by CPIB is inhibited by chloramphenicol. No increase in microbodies occurred in female rats or in chickens, guinea pigs, or rabbits at the dosage used (0.25% in diet). No changes in microbodies were seen in monkey liver. Catalase activity was generally parallel to the numerical response in microbodies. Additional observations suggest that the microbody response to CPIB is not related to hepatomegaly induced by this agent but may be related to the hypolipidemic effect of CPIB, though hypolipidemia per se is not a specific or sufficient cause of microbody proliferation.

scholarly journals Phenolic Profiling and Evaluation of Contraceptive Effect of the Ethanolic Extract ofSalsola imbricataForssk. in Male Albino Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Naglaa Gamil Shehab ◽  
Eman Abu-Gharbieh
Keyword(s):  
Oral Administration ◽  
Methanol Extract ◽  
Ethanolic Extract ◽  
Biological Evaluation ◽  
Male Rats ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Male Contraceptive ◽  
Contraceptive Effect ◽  
Phenolic Components

Reported researches dealing with either composition or bioactivity ofSalsola imbricataare limited. This study was conducted aiming to investigate the phenolic composition of the plant and evaluate its efficacy as male contraceptive. Polyphenols, namely, phenolic acids and flavonoids, were qualitatively and quantitatively analysed by RP-HPLC in the hydrolysed methanol extract using two different wavelengths, 280 and 330 nm. The efficiency of different solvents in extracting the plant phenolics was assessed via spectrophotometric determination of the total phenolic and flavonoid contents. Acute toxicity study was carried out on the ethanolic extract to ascertain its safety prior to biological evaluation. The contraceptive effect was assessed, in male rats, by oral administration of the extract at two doses (250 and 500 mg/kg b. wt.), over a period of 65 days. HPLC analyses allowed the identification and quantification of a total of 13 and 8 components in the hydrolysed-methanol extract; the overall phenolic composition was dominated by quercitrin (12.692%) followed by coumaric acid (4.251%). Prolonged oral administration of the ethanolic extract caused slight reduction in the testis weight only. A significant decrease in the sperm count was observed(P<0.01)in the two treated groups while significant decrease in the epididymal sperm motility was only observed in the high dose group. Morphological abnormalities were observed in sperms of treated animals. No distinct change in serum FSH, LH, and testosterone concentration was recorded. The histopathological findings supported to a high extent these results. The male contraceptive activity ofSalsola imbricatacould be ascribed to its phenolic components, especially quercitrin.

Daily exercise and gender influence postexercise cardiac autonomic responses in hypertensive rats

1997 ◽  
Vol 272 (3) ◽  
pp. H1412-H1418 ◽  
Author(s):  
Y. Chen ◽  
M. P. Chandler ◽  
S. E. DiCarlo
Keyword(s):  
Acute Exercise ◽  
Female Rats ◽  
Male Rats ◽  
Autonomic Response ◽  
Hypertensive Rats ◽  
Experimental Conditions ◽  
Autonomic Responses ◽  
Gender Influence ◽  
Single Bout ◽  
And Gender

The influence of daily spontaneous running (DSR) and gender on postexercise cardiac autonomic responses was examined in spontaneously hypertensive rats. Rats were weaned at 4-5 wk of age and were randomly assigned to a sedentary (7 males and 6 females) or DSR (7 males and 8 females) group. After 8 weeks of DSR or sedentary control, rats were chronically instrumented with arterial and venous catheters. After 5 days of recovery, cardiac sympathetic (ST) and parasympathetic tonus (PT) were determined (by the response of heart rate to receptor antagonists) on alternate days under two experimental conditions: no exercise and postexercise. After a single bout of dynamic treadmill exercise (12 m/min, 10% grade for 40 min) ST was reduced (P < 0.05) (male sedentary: no exercise 45 +/- 4 vs. postexercise 28 +/- 3 beats/min; female sedentary: no exercise 69 +/- 10 vs. postexercise 37 +/- 7 beats/ min). PT was also altered after exercise (male sedentary: no exercise -31 +/- 4 vs. postexercise -11 +/- 2 beats/min; female sedentary: no exercise -5 +/- 4 vs. postexercise 7 +/- 4 beats/min). After DSR, ST was reduced (male sedentary 45 +/- 4 vs. DSR 22 +/- 3 beats/min; female sedentary 69 +/- 10 vs. DSR 36 +/- 4 beats/min) (P < 0.05). Finally, male rats had a lower ST and higher PT than female rats. These results demonstrate that 1) ST was reduced after a single bout of dynamic exercise; 2) ST was reduced after DSR; 3) the autonomic response to acute exercise was attenuated after DSR; and 4) there was a gender influence on the cardiac autonomic function.

A COMPARISON OF CHANGES IN THE PERIPHERAL PLASMA CONCENTRATIONS OF LUTEINIZING HORMONE AND FOLLICLESTIMULATING HORMONE IN THE RAT

1975 ◽  
Vol 65 (3) ◽  
pp. 389-397 ◽  
Author(s):  
K. BROWN-GRANT ◽  
FENELLA GREIG
Keyword(s):  
Plasma Concentrations ◽  
Fold Increase ◽  
Early Response ◽  
Female Rats ◽  
Male Rats ◽  
Postnatal Life ◽  
Experimental Conditions ◽  
Releasing Factors ◽  
Secretion Rates ◽  
Sustained Increase

SUMMARY Plasma FSH concentrations in rats have been determined by radioimmunoassay under a variety of experimental conditions to see whether any evidence could be obtained of an acute divergence in LH and FSH secretion rates which would support the idea of separate, specific hypothalamic releasing factors for these two hormones. During the normal ovarian cycle and after the administration of progesterone to female rats on the morning of the day of pro-oestrus increased secretion of both LH and FSH began simultaneously but FSH concentrations were later maintained or increased slightly while LH concentrations were falling. During early pregnancy FSH concentrations were higher than at the corresponding stage of the cycle at a time when LH concentrations had been shown to be lower. Progesterone injected at the dioestrous stage of the cycle reduced both LH and FSH concentrations though the effect on LH was more marked. After ovariectomy at any stage of the oestrous cycle or on day 4 of pregnancy there was a rapid and significant increase in plasma FSH concentration which was quite different from the delayed increase in LH concentration observed in these animals. In contrast, the early increase in FSH concentration in male rats after castration was less than the increase in LH concentration. The final FSH concentration in castrated males was only about four times the basal level in contrast to the 10- to 15-fold increase in LH in males and both LH and FSH in females. Anovulatory adult females that had received 1·25 mg of testosterone propionate on day 4 of postnatal life showed the rapid and sustained increase in plasma FSH after ovariectomy that was seen in normal females. None of these results strongly support the idea that separate and specific hypothalamic releasing factors for LH or FSH are secreted in the rat although the differences in the early response to gonadectomy could be explained on this basis.

scholarly journals Sambiloto (Andrographis paniculata Nees.) leaf extract activity as an α-Amylase enzyme inhibitor

2021 ◽  
pp. 305-308
Author(s):  
Yustina Sri Hartini ◽  
Dewi Setyaningsih ◽  
Maria Josephine Vivian Chang ◽  
Maria Cyrilla Iglesia Adi Nugrahanti
Keyword(s):  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Enzyme Inhibitor ◽  
Statistical Tests ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Andrographis Paniculata ◽  
Activity Test ◽  
Ic50 Value

Introduction: Sambiloto (Andrographis paniculata) is an antidiabetic medicinal plant that acts by inhibiting the α-amylase enzyme. Andrographolide, the active compound of sambiloto leaf, is insoluble in water but dissolves in ethanol. Aim: This study compared the in vitro activity of aqueous extract and ethanolic extract of sambiloto leaf with the α-amylase enzyme. Methods: The inhibitory activity test of the α-amylase enzyme was carried out using the ultraviolet-visible spectrophotometric method by measuring the absorbance of the remaining starch, which forms a blue complex with iodine-iodide. Results: The inhibitory activity of the α-amylase enzyme of the aqueous extract of sambiloto leaf (with the IC50 value of 14.203 ± 0.112 mg/mL) was lower than that of the ethanol extract (with the IC50 value of 9.253 ± 0.116 mg/mL). The results of the statistical tests showed significant differences (p <0.05) between the inhibitory activity of the α-amylase enzyme acarbose and the activity of both extracts.

scholarly journals Incision Wound Healing Test of Ethanolic Extract Gel from Salaon (Parsonsia alboflavescens [Dennst.] Mabb.) Leaves in Male Rats

2021 ◽  
Vol 9 (A) ◽  
pp. 776-781
Author(s):  
Nilsya Zebua ◽  
Muharni Saputri ◽  
Winda Giovana Sijabat ◽  
Inda Aristika Sri Retno Wulandari ◽  
Ira Nofriani ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Male Rats ◽  
Optimum Dose ◽  
Significant Difference ◽  
The One ◽  
Post Hoc ◽  
Incision Wound

BACKGROUND: An incision wound is a wound caused by a sharp object. One of the plants that can be used as a traditional medicine for an incision wound is salaon leaves. The prepared form chosen is the gel because it is easy to use and its distribution is faster on the skin. AIM: The aim of the study was to explore whether salaon leaf extract gel meets the quality evaluation requirements and to know the effectiveness of ethanol extract gel of salaon (Parsonsia alboflavescens [Dennst] Mabb.) leaves to cure a scar on male rats. METHODS: Experimental method with salaon leaves as sample. Simplicia of salaon leaves extracted by maceration method, and then arenga gum gel-based formulation, afterward evaluation, and testing of its effectiveness on the healing of incision wound of male rats. Normal data were analyzed by the One Way Analysis of variance (ANOVA) and Post Hoc Tuckey Method. Kruskal-Wallis test used for abnormal data. RESULTS: The whole formula shows homogenous results, normal pH, stability, dissemination and good viscosity, less irritating, and with good adhesion. The results of a statistical analysis of the wound on the 8th day with ANOVA test indicate a significant difference between the five groups p = 0.000 (p < 0.05) and Post-Hoc Tukey Honest Significant Difference test results obtained a concentration of 5% not significantly different from the positive control Placenta extract gel. The optimum dose that can heal incision wound in male rats is 5%. CONCLUSION: The whole formulation qualifies for the quality of the gel formulation. Ethanol extract gel of salaon leaves can heal wound on male rats and the optimal dose is 5%.

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