Potensi Ekstrak Buah Buni (Antidesma bunius (L) Spreng) Sebagai Inhibitor Enzim α-Glukosidase

Diabetes mellitus is still a global health problem that continues to increase rapidly and become one of the major metabolic diseases throughout the world. This study aims to determine the potential of Buni fruit as an α-glucosidase inhibitor. α-glucosidase inhibition test is carried out on a blank solution (test solution without sample/standard), acarbose solution as a comparison standard and samples are carried out in accordance with the optimization conditions obtained. The rendemen percent of green and red buni fruit extract yields are 6.35% and 3.09%, respectively. The results of the identification of secondary metabolites using TLC showed that green and red buni fruit extract contains flavonoid, phenolic and alkaloid compounds. The results of the α-glucosidase enzyme inhibition test showed that the red buni fruit extract had the highest activity compared to green buni fruit extract with an IC50 value of 85.27 ppm.

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Anti-diabetic effect of aqueous fruit extract of Borassus aethiopum (Mart.) in alloxan-induced diabetic rats

International Journal of Phytomedicine ◽

10.5138/09750185.1884 ◽

2016 ◽

Vol 8 (3) ◽

pp. 384 ◽

Cited By ~ 1

Author(s):

Christopher Larbie ◽

Jeremiah Issaka ◽

Marina Tandoh

Keyword(s):

Body Weight ◽

Metabolic Diseases ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Fruit Extract ◽

Chronic Hyperglycemia ◽

The World ◽

Acute Study ◽

Phytochemical Constituents ◽

Borassus Aethiopum

Introduction: Diabetes mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The chronic hyperglycemia is associated with long-term damage, dysfunction, and failure of various organs, especially the eyes, kidneys, nerves, heart, and blood vessels and is among the top ten causes of death in the world. Borassus aethiopum Mart. (family Arecaceae) is a plant species of Borassus palm found widely across Africa. It serves an important source of food, providing edible fruits, and nuts, and also has a number of pharmacological uses that have been reported in some parts of the world. Objective: The current study was aimed at assessing the phytochemical constituents as well as the antidiabetic and hypolipidaemic effect of fruit extract of B. aethiopum in alloxan-induced diabetic rats. Materials and Methods: The fruits extract was prepared (FEB) and phytochemical constituents evaluated using standard methods. The antidiabetic and hypolipidaemic properties in alloxanized rats assessed for 7 and 28 days. Normoglycaemic and alloxan-induced diabetic rats were treated with FEB at doses of 100 mg, 250 mg and 500 mg/kg body weight. Observations on body weight, relative organ weight, haematological and biochemical parameters were measured in both acute and sub-chronic circumstances. Results: The presence of tannins, saponins, glycosides, triterpenoids and alkaloids were detected. Fasting blood glucose was reduced significantly (p<0.05) in diabetic rats in acute study at a dose of 500 mg/kg body weight and at 250 mg and 500 mg/kg body weight in sub-chronic studies. WBC and PLT levels were significantly increased after treatment with 500 mg/kg body weight. Urea and ALT levels also reduced significantly in both acute and sub-chronic studies. Conclusion: The aqueous fruit extract of B. aethiopum is antidiabetic. It was also found to be nephron- and hepato-protective as well as boosting the immunity of the animals.

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Use of Phosphatidylcholine in the Treatment of Localized Fat Deposits. Results and expectations

Revista de Chimie ◽

10.37358/rc.17.7.5691 ◽

2017 ◽

Vol 68 (7) ◽

pp. 1438-1441 ◽

Cited By ~ 1

Author(s):

Sorin Berbece ◽

Dan Iliescu ◽

Valeriu Ardeleanu ◽

Alexandru Nicolau ◽

Radu Cristian Jecan

Keyword(s):

World Health Organisation ◽

Normal Weight ◽

World Health ◽

Basic Substance ◽

Physical Exercises ◽

Fat Deposits ◽

The World ◽

The Common ◽

Global Health Problem ◽

Over Time

Obesity represents a global health problem. According to the latest studies released by the World Health Organisation (WHO), 1.7 billion currently in excess of normal weight individuals, of which approx. 75% are overweight (body mass index - BMI 25 to 30). The common form of excess adipose tissue manifestation in overweight individuals is localized fat deposits with high (abdominal) or low (buttocks and thighs) disposition. Although the overweight can be corrected relatively easy by changing behavioral habits or food, a constant physical exercises program or following a diet food are not accessible to all through the efforts of will, financial and time involved. Several methods have been studied and tested over time to eliminate more or less invasive fat deposits with varying efficacy and adverse effects. Chemical lipolysis using phosphatidylcholine as the basic substance was initially used in hypercholesterolemia and its complications and was rapidly adopted in mesotherapy techniques for the treatment of fat deposits. This study reveals the results obtained using Dermastabilon on a sample of 16 patients, the time allocated to treatment and discomfort being minimal, and rapid and notable results. There were no side effects.

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Potency of Super Red Dragon Fruit Flesh Extract (Hylocereus costaricencis) in Herbal Lipstick as Colorant, Antioxidant and Antibacterial

SCISCITATIO ◽

10.21460/sciscitatio.3 ◽

2020 ◽

Vol 1 (1) ◽

Author(s):

Lusiana Dian Anjarsari ◽

Dwi Aditiyarini ◽

Guntoro

Keyword(s):

Staphylococcus Aureus ◽

Chemical Compound ◽

Negative Impact ◽

Antioxidant Properties ◽

Natural Source ◽

Fruit Extract ◽

Fruit Flesh ◽

Self Confidence ◽

Dragon Fruit ◽

Ic50 Value

Lipstick is one kind of cosmetics which is used as lips colorant to increase self-confidence. Nowadays, lipsticks from natural source is popular to reduce the negative impact of chemical compound or synthetics colorant in lipstick intensively for health. Super red dragon fruit is one kind of natural ingredients which can be used as colorant for lipstick. Moreover, this fruit is rich of antioxidant and antibacterial component that is good for skin health. In this research, extract of super red dragon fruit flesh was added in the lipstick during preparation with variation of concentration 0, 10, 20, 30 and 40%. Antioxidant analysis resulted the IC50 value in 81.55% indicating the strong antioxidant properties. Furthermore, the concentration of dragon fruit extract 40% was able to inhibit the growth of Staphylococcus aureus bacteria.

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EFEK SINERGIS KLOROKUIN DAN N-ACETYL CYSTEINE TERHADAP PENURUNAN PARASITEMIA DAN PENURUNAN KADAR MALONDYALDEHYDE ERITROSIT MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v20i1.439 ◽

2016 ◽

Vol 20 (1) ◽

pp. 6

Author(s):

Agustin Iskandar ◽

Sudjari Sudjari

Keyword(s):

Body Weight ◽

Global Health ◽

Experimental Research ◽

Plasmodium Berghei ◽

Synergic Effect ◽

The World ◽

Parasitemia Level ◽

Acetyl Cysteine ◽

Combined Drugs ◽

Global Health Problem

Malaria is still a global health problem around the world particularly in Indonesia. Chloroquine is one of the anti-malarial drugs which is still used in Indonesia. The aim of this research is to know the synergic effect of Chloroquine and N-Acetyl Cysteine towards parasitemia and erythrocyte malondyaldehyde levels in mice infected with Plasmodium berghei. This was an experimental research using Plasmodium berghei and BALB/c strain mice, 6−8 weeks old, with body weight 20−30 grams. After infected with Plasmodium berghei, the mice were divided into eight (8) groups: control, chloroquine, NAC 0.25 mg/gBb; NAC 0.5 mg/gBb; NAC 1 mg/gBb and combined drugs of chloroquine and NAC 0.25 mg/gBb; NAC 0.5 mg/gBb; NAC 1 mg/gBb. On the 3rd, 5th, and 7th day after treatment, the mice were killed and their parasitemia and Malondialdehyde (MDA) levels were measured. By using MANOVA and Tukey HSD test, a signifi cant difference in parasitemia level was found between the control and chloroquine group, as well as the control and combined drug groups. In the NAC groups, there was no decrease. The decrease of MDA level began on the 7th day. It can be concluded that there was a synergic effect between the chloroquine and N-Acetyl Cysteine in decreasing parasitemia and malondyaldehyde levels in mice BALB/c infected with Plasmodium berghei.

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Pengaruh pemberian obat anti tuberkulosis dengan vitamin D terhadap perubahan BTA pada penderita tuberkulosis paru di Kabupaten Pidie

Jurnal SAGO Gizi dan Kesehatan ◽

10.30867/gikes.v2i1.442 ◽

2021 ◽

Vol 2 (1) ◽

pp. 25

Author(s):

Rina Maulidar ◽

Nurjannah Nurjannah ◽

Aulina Adamy ◽

Iskandar Iskandar

Keyword(s):

Vitamin D ◽

Drug Therapy ◽

Intervention Group ◽

Control Group ◽

Significance Level ◽

The World ◽

Post Test ◽

And Control ◽

Global Health Problem ◽

Test Control

Background: Tuberculosis (TB) is a global health problem, Indonesia had the second rank of the case in the world after India which is the fourth cause of death. Giving vitamin D together with anti-tuberculosis drugs can increase healing proses because vitamin D (anti-microbial immunomodulators) kills Mycobacterium tuberculosis.Objectives: This study aims to measure the effect of giving anti-tuberculosis drugs with vitamin D on changes in AFB in patients with pulmonary tuberculosis.Methods: This study was used a quasi-experiment design, pre-test and post-test control group was doing at 25th March – 25th July 2019. This research has been conducted in Pidie Regency. The intervention group (17 samples with tuberculosis drugs) was given Softgels vitamin D 5000 IU for 4 months, while the control group was only anti-tuberculosis drug therapy. The data were analyzed statistically using the Wilcoxon and Mann Whitney tests at a significance level of 95%.Results: Before the intervention, the BTA status of both the intervention and control group was similar (p= 0.061). After the intervention, there was a decrease in AFB interactions (p= 0.000). There was a decrease in the contribution of AFB in the control group before with after intervention (p= 0.000). There are those who support (p= 0.033) giving vitamin D the acceleration of pulmonary tuberculosis treatment. Conclusion: It is better to administer vitamin D to the successful treatment of pulmonary TB in patients undergoing anti-tuberculosis drug therapy.

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Potential Effect of Pomegranate Peels Extract (Punica Granatum.) against Covid-19 Virus .

10.21203/rs.3.rs-474809/v1 ◽

2021 ◽

Author(s):

Ashraf fawzy mosa ◽

Mostafa abo Elhoda Mohamed

Keyword(s):

Tissue Culture ◽

Water Extract ◽

Punica Granatum ◽

Inhibitory Effect ◽

Potential Effect ◽

Laboratory Methods ◽

Global Epidemic ◽

Ic50 Value ◽

The World

Abstract Background: Covid-19 Virus infection poses significant global health challenges and considered a global epidemic sweeping all countries of the world Which prompted scientists around the world to search for a quick or safe treatment to preserve people's lives .So far, options for controlling and treating the disease have not been revealed. The current study was conducted to evaluate the effectiveness of pomegranate peels extract against the Covid-19 virus in the laboratory. Methods: In this research, tow methods of extraction are carried out ethyl alcohol and distal water extract of pomegranate peels . activity of the extract assessed using 50% Tissue Culture Infectious Doses (TCID50) method in Vero E6 cells. Results: Pomegranate peels extract had the highest inhibitory effect against Covid -19 virus with IC50 value of 0.125, 0.0625 and 0.031256 μl in Vero E6 cells. Conclusion: Based on our results, the aqueous extract of pomegranate peels can inhibit Covid-19 virus replication in vitro.

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Phaleria macrocarpa Fruit Extract Inhibits NF-ĸB Activation and Apoptosis Induction on HeLa Cells

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev8iss1pp9-14 ◽

2017 ◽

Vol 8 (1) ◽

pp. 9

Author(s):

Meirizky Zulharini S. ◽

Amalia Miranda ◽

Lina Permatasari ◽

Hilyatul Fadliyah ◽

Riris Istighfari Jenie

Keyword(s):

Transcription Factor ◽

Molecular Docking ◽

Mtt Assay ◽

Apoptosis Induction ◽

Double Staining ◽

Fruit Extract ◽

Apoptosis Inhibition ◽

Ic50 Value ◽

Anticancer Mechanism ◽

Phaleria Macrocarpa

NF-κB is a transcription factor and if activated, it induces apoptosis inhibition. Phalerin from Phaleria macrocarpa fruits expected to inhibit NF-κB activation. This research is to investigate anticancer mechanism of Phaleria macrocarpa fruits extracts (EBMD) in NF-κB pathway. Molecular docking assay was performed to determine phalerin affinity to IKK. Cytotoxic activity was observed by MTT assay. Double staining was performed to determine the apoptotic cells. Docking score of phalerin to IKK is -60. The IC50 value of EBMD is 629 μg/mL. Apoptosis profile shows (shown that) many cells undergoing apoptosis after treatment. Thus, EBMD potentially inhibits activation of NF-κB pathway and triggers apoptosis on HeLa cells.Keywords : NF-κB, Phaleria macrocarpa, sel HeLa, Bcl-2, IKK, molecular docking

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ANTIMICROBIAL, ANTIRADICAL AND INSECTICIDAL ACTIVITY OF GARDENIA GUMMIFERA L. F. (RUBIACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i10.20252 ◽

2017 ◽

Vol 9 (10) ◽

pp. 265 ◽

Cited By ~ 1

Author(s):

Prashith Kekuda T. R. ◽

Raghavendra H. L. ◽

Shilpa M. ◽

Pushpavathi D. ◽

Tejaswini Petkar ◽

...

Keyword(s):

Antibacterial Activity ◽

Insecticidal Activity ◽

Leaf Extract ◽

Antiradical Activity ◽

Radical Scavenging ◽

Fruit Extract ◽

Fruit Extracts ◽

Ic50 Value ◽

Agar Well Diffusion Assay ◽

Dose Dependent

Objective: The present study was carried out to investigate antimicrobial, antiradical and insecticidal potential of leaf and fruit of Gardenia gummifera L. f. (Rubiaceae).Methods: The leaf and fruits were shade dried, powdered and extracted by maceration process using methanol. Antibacterial activity was evaluated against Gram positive and Gram negative bacteria by Agar well diffusion assay. Antifungal activity was determined against six seed-borne fungi by Poisoned food technique. Antiradical activity of leaf and fruit extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) radical scavenging assays. Insecticidal activity of leaf and fruit extracts, in terms of larvicidal and pupicidal activity, was assessed against larvae and pupae of Aedes aegypti.Results: Both the extracts inhibited all test bacteria. Marked antibacterial activity was displayed by fruit extract when compared to leaf extract. S. epidermidis and E. coli were inhibited to highest and least extent by both extracts respectively. Fruit extract was found to exhibit higher antifungal effect when compared to leaf extract. Leaf extract and fruit extract exhibited highest inhibitory activity against A. niger and A. flavus respectively. Leaf and fruit extracts scavenged DPPH radical’s dose dependently with an IC50 value of 49.01µg/ml and 2.53µg/ml respectively. The scavenging of ABTS by leaf and fruit extracts was dose dependent and the IC50 value for leaf and fruit extract was 2.58µg/ml and 2.31µg/ml respectively. Fruit extract was shown to exhibit marked antiradical activity when compared to leaf extract. Leaf and fruit extracts exhibited dose dependent insecticidal activity in terms of larvicidal and pupicidal activity and the susceptibility of larvae and pupae to extracts was in the order II instar larvae>IV instar larvae>pupae. Fruit extract displayed marked insecticidal potential when compared to leaf extract.Conclusion: Overall, fruit extract of G. gummifera exhibited marked antimicrobial, antiradical and insecticidal activity when compared to leaf extract. The plant can be used for developing agents/formulations effective against infectious microorganisms, oxidative stress and insect vectors that transmit dreadful diseases. The observed bioactivities could be ascribed to the presence of active principles which are to be isolated and characterized.

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Characterization of α-Glucosidase Inhibitor/Cyclodextrin Complex Prepared by Freeze-Drying

Journal of Pharmaceutics ◽

10.1155/2018/3202719 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9

Author(s):

Yutaka Inoue ◽

Sachie Narumi ◽

Akiho Mitsumori ◽

Isamu Murata ◽

Ikuo Kanamoto

Keyword(s):

Blood Glucose ◽

Intermolecular Interaction ◽

Freeze Drying ◽

Inhibition Test ◽

Molar Ratio ◽

Cyclodextrin Complex ◽

Glucosidase Inhibitor ◽

Activity Inhibition ◽

Spectrum Measurement ◽

Freeze Dried

Miglitol (MT) is an α-glucosidase inhibitor with a postmeal blood glucose level lowering effect that is used to treat type 2 diabetes. In addition, α-cyclodextrin (αCD) has been reported to inhibit increases in postmeal blood glucose. The aim of this study was to prepare a freeze-dried product (FD) composed of MT and αCD or γCD (molar ratio of MT/αCD = 1/1, MT/γCD = 1/1) and to evaluate the physicochemical properties and biological activity of the FD. The PXRD profile of FD exhibited a halo pattern, and characteristic peaks derived from MT, αCD, and γCD were not observed. The TG-DTA results for FD indicated an increased weight loss temperature and the absence of an endothermic peak for MT. The NIR absorption spectrum measurement suggested an intermolecular interaction between MT and αCD or γCD in the FD. 1H-1H NOESY NMR spectroscopy (D2O) revealed an intermolecular interaction in the FD. The results of the α-glucosidase activity inhibition test and the α-amylase activity inhibition test indicated that the FD exhibited the same inhibition rate as MT alone and the effects of MT were not altered by the freeze-drying method.

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Uji Kualitatif dan Uji Aktivitas Antioksidan Ekstrak Etanolik Buah Maja (Aegle Marmelos (L.)Correa) dengan Metode DPPH

Jurnal Riset Farmasi ◽

10.29313/jrf.v1i1.25 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-8

Author(s):

Muhammad Nur Fauzi

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Vitamin C ◽

Aegle Marmelos ◽

Fruit Extract ◽

Fruit Extracts ◽

The Third ◽

Dpph Method ◽

Fruit Samples ◽

The World

Abstract. Indonesia is a country with the third largest tropical forest in the world. The number of medicinal plants in Indonesia is estimated to be around 1,260 types of plants. Plants produce secondary metabolites that have potential as antioxidants. One of the plants that contains a lot of secondary metabolites is maja (Aegle marmelos (L.) Corr). The purpose of this study was to determine the content of secondary metabolites in maja fruit extracts and to determine the antioxidant activity contained in maja fruit extracts using the DPPH method. The research was conducted by extracting maja fruit samples using maceration method to obtain a thick extract. The extracts obtained were tested for secondary metabolites, TLC test, and antioxidant activity tests using the DPPH method using Uv-vis spectrophotometry. The results of this study indicate that maja fruit extract contains secondary metabolites of flavonoids, tannins, alkaloids, saponins, and glycosides. TLC results obtained Rf 0.512. The result of antioxidant activity of maja fruit extract obtained by IC50 was 269.153 µg / mL. and IC50 vitamin C as a comparison obtained 28,907µg / mL. This shows that the antioxidant activity of maja fruit extract is smaller than the antioxidant activity of vitamin C. Abstrak. Indonesia adalah negara dengan hutan tropis paling besar ketiga di dunia, Jumlah tumbuhan berkhasiat obat di Indonesia diperkirakan sekitar 1.260 jenis tumbuhan. Tumbuhan menghasilkan metabolit sekunder yang berpotensi sebagai antioksidan. Salah satu tanaman yang banyak mengandung metabolit sekunder adalah tanaman maja (Aegle marmelos (L.) Corr). Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder pada ekstrak buah maja dan untuk mengetahui aktivitas antioksidan yang terdapat pada ekstrak buah maja dengan metode DPPH. Penelitian dilakukan dengan mengekstraksi sampel buah maja dengan metode maserasi untuk memperoleh ekstrak kental. Ekstrak yang diperoleh dilakukan uji metabolit sekunder, uji KLT, dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri Uv-vis. Hasil penelitian ini menunjukkan bahwa ekstrak buah maja mengandung metabolit sekunder flavonoid, tanin, alkaloid, saponin, dan glikosida. Hasil KLT diperoleh Rf 0,512. Hasil aktivitas antioksidan ektrak buah maja yang diperoleh dengan IC50 adalah 269,153 µg/mL. dan IC50 vitamin c sebagai pembanding diperoleh 28,907µg/mL. hal ini menunjukan bahwa daya aktivitas antioksidan ekstrak buah maja lebih kecil dibanding dengan daya aktivitas antioksidan vitamin C. Kata Kunci: , ,

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