scholarly journals NATURAL RESISTANCE AND DEVELOPMENT OF EGG-TYPE REPAIR YOUNG CHICKS

2021 ◽  
Vol 246 (2) ◽  
pp. 132-136
Author(s):  
А.А. Menkova ◽  
◽  
V.N. Shilov ◽  
Е.М. Tsygankov ◽  
◽  
...  
Keyword(s):  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
Natural Resistance ◽  
Poultry Farm ◽  
Immune Protection ◽  
Poultry House ◽  
Blood Neutrophils ◽  
And Control ◽  
Experimental Group

Blood and lymph make up the circulatory link of the bird's immune system. It is necessary to note the humoral factor of immune protection-lysozyme. The purpose of scientific research is to study the level of natural resistance and development of egg-type chickens after aerosol application of the disinfectant Argodez. Research and production experiments were conducted in the conditions of the poultry farm "snow", Bryansk region, p. Voucher. Aerosol disinfection was performed in the poultry house before settling in with day-old chickens. Test shop were treated once Arcadetm of 0.01%, the rate of 2 ml/m3, and control Desalina - f - 5 ml/m3., with the generator cold fog IGEBA -UNIPRO-5. At the age of 24 hours, two groups of egg - type chickens, the Lohmann-brown cross, were formed using the method of analogous groups. Blood was taken from the underwing vein and by decapitation, before feeding, at the daily, 30-day, 60-day age. Bactericidal, lysozyme, and phagocytic activity of blood serum was determined according to generally accepted methods. Control weighing of chickens of both groups was performed on days 30 and 60 of the study. A significant increase in phagocytic activity of blood neutrophils was found in the experimental group of chickens on the 30th-21.40 % and 60th day of research-25.40 %, as well as a tendency to increase the bactericidal activity of blood serum by 1.90 % and 7.90 % and the lysozyme activity of blood serum by 8.46 % and 9.17 %. It was also found to increase safety by age periods. The highest safety was observed at the age of 1-30 days and was 99.50 %, then 30-60 – 99.10 %.

scholarly journals The cell protection of weated pigs for probiotics

2020 ◽  
pp. 111-119
Author(s):  
L. Bondarenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
The State ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Control Group ◽  
Stressful Situation ◽  
Experimental Group

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

PREVALENCE OF PNEUMOCYSTIS JIROVECII IN FREQUENTLY ILL CHILDREN ON ADMISSION TO THE RESPIRATORY DEPARTMENT AND DURING SANATORIUM REHABILITATION

2021 ◽  
Vol 100 (2) ◽  
pp. 104-111
Author(s):  
L.V. Feklisova ◽  
◽  
N.V. Karazhas ◽  
M.K. Khadisova ◽  
E.I. Likhanskaya ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Active Form ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Pharyngeal Wall ◽  
Blood Neutrophils ◽  
Pneumocystis Infection ◽  
Chain Fatty Acids

The aim of the study was to determine the frequency of occurrence and to assess the significance of markers of pneumocystosis in frequently ill children (FIC) during inpatient treatment and during rehabilitation in a sanatorium. Materials and methods of research: 115 children with recurrent respiratory diseases were observed: 52 were treated in a hospital and 63 underwent rehabilitation in a sanatorium. Biological samples (oropharyngeal swabs, saliva, feces and blood serum) were examined for pneumocystosis. Laboratory techniques used: a polymerase chain reaction (PCR) to detect DNA pathogens, an indirect immunofluorescence reaction (NRIF) – antigens, and immunoforment analysis (IFA) – specific antibodies of immunoglobulins (Ig) of classes M (IgM) and G (IgG). The material for laboratory research was smears from the posterior pharyngeal wall (PCR, NRIF) and blood serum (IFA). Microbiological studies included seeding, isolation of cultures with quantitative counting of colony-forming units in samples of oropharyngeal swabs, feces. Determination of the functional activity of the oropharynx microbiocenosis by the concentration of short-chain fatty acids in saliva by means of GLC method. Phagocytic activity of blood neutrophils was also investigated. Results: in hospital patients, markers for pneumocystis were found in 60% of cases, including the active form (40%) and pneumocystis (13%); in the sanatorium – 33% of cases, with active infection (26%) and detection of pneumocystis (17%). Inpatients were diagnosed with clinical variants of pneumocystis infection: pneumonia, obstructive bronchitis, acute respiratory infections; schoolchildren have been diagnosed with active pneumocystis infection and carriage. Dysbiotic disorders of the loci of the oropharynx and intestines were identified in all examined subjects, inhibition of the phagocytic activity of blood neutrophils and shifts in the concentration of short-chain fatty acids have been observed. Conclusion: a high proportion of active pneumocystis infection (40% and 27% of cases) was revealed in FIC treated in the hospital and in the sanatorium, which represents a risk group for infection/reinfection with pneumocystosis in the premises.

scholarly journals STUDIES ON PNEUMOCOCCUS GROWTH INHIBITION

1927 ◽  
Vol 46 (2) ◽  
pp. 239-262 ◽  
Author(s):  
Oswald H. Robertson ◽  
Richard H. P. Sia
Keyword(s):  
Guinea Pig ◽  
Growth Inhibition ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
The Other ◽  
Natural Resistance ◽  
Essential Difference ◽  
Defence Mechanism ◽  
Fresh Serum ◽  
Killing Action

A study was made of the pneumococcidal action of serum-leucocyte mixtures of pneumococcus-resistant animals with a view to determining whether this property of the blood is to be accounted for by the presence of certain serum constituents or by cellular characteristics which are lacking in the blood of susceptible animals. By means of a method specially developed for this purpose, it was found that, after adequate contact with the serum of pneumococcus-resistant animals, virulent pneumococci were phagocyted actively not only by the homologous leucocytes but also by the leucocytes of other resistant and susceptible animals. On the other hand, pneumococci exposed to the action of the serum of pneumococcus-susceptible animals were not taken up by the leucocytes of either the resistant or susceptible species. All the resistant animals tested, dog, cat, sheep, pig and horse, showed marked opsonic properties in their blood serum which were not found in the serum of susceptible ones, rabbit, guinea pig and human. There appeared, however, to be no essential difference in the phagocytic activity of the leucocytes from the various animals. It was then shown that the pneumococcus-destroying power of serum-leucocyte mixtures was entirely abolished when heated serum was substituted for fresh serum and that such heated serum had lost much of its opsonic potency. Neither the living leucocytes alone nor extracts of the leucocytes were observed to exert any killing action on pneumococci. Further evidence of the controlling influence of opsonic action in the antipneumococcus defence mechanism of the blood, and its importance in natural resistance, was afforded by a study of the opsonin content and leucocytic functions of the blood of full grown and young rabbits as related to their widely varying degrees of pneumococcus susceptibility.

scholarly journals Effects of biologically active substances on interior indicators of pigs in the early postnatal period

2019 ◽  
pp. 69-72
Author(s):  
Olga Nikolaevna Polozyuk ◽  
Tatyana Ivanovna Lapina
Keyword(s):  
Gastrointestinal Tract ◽  
Blood Serum ◽  
Dietary Supplements ◽  
Postnatal Period ◽  
Biologically Active ◽  
Control Group ◽  
Control Groups ◽  
And Control ◽  
Experimental Group ◽  
Active Substances

The authors found out that the application  of dietary supplements "Glimalask Lact" allowed preventing the disorder of the gastrointestinal tract and saving all piglets throughout the experiment.  After application of "Agrocid super oligo", the safety of purebred piglets was 100 %, and for two pedigree piglets it was 96.7%.   In piglets of the 1st and 2nd control groups, disorder of the gastrointestinal tract was noted during the completion of colostral immunity and at weaning from sows.  The safety of purebred piglets was 96.7%, and of the two pedigree piglets  it was 93.4%. The number of γg-globulins in the blood serum of two breed piglings treated with the Glimalask Lact supplement increased by 3.5 and 2.5% compared to the piglets of the experimental group fed with Agrocid super oligo, and of the control group, The number of g-globulins in the blood serum of purebred  piglets it increased by 2.4 and 3.1% compared with analogues of the experimental and control groups.

scholarly journals THE EFFECT OF «ARCUSIT» ON BIOCHEMICAL AND IMMUNOLOGICAL PARAMETERS BLOOD SERA IN SILVER-BLACK FOX (SILVER-BLACK FOX)

2021 ◽  
Vol 247 (3) ◽  
pp. 192-197
Author(s):  
I.I. Okulova ◽  
◽  
I.A. Domsky ◽  
Yu.A. Berezina ◽  
Z.N. Beltyukova ◽  
...  
Keyword(s):  
Experimental Data ◽  
Blood Serum ◽  
Bactericidal Activity ◽  
Lysozyme Activity ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Immunological Parameters ◽  
Nonspecific Resistance ◽  
Experimental Group

The use of «Arkusit» at a dose of 20 μg / kg of body weight indicates a beneficial effect on the factors of nonspecific resistance of the silver-black fox. The concentration of alkaline phosphatase in the blood serum in the experimental group decreased by 1.8 times (P <0.001), the ALT index - by 1.2 (P <0.05). The concentration of glucose in the experimental group increased 2 times (P <0.05) compared with the control group. In animals of the experimental group, the lysozyme activity of blood serum increased 1.3 times (P <0.05), the OPR reaction and the phagocytic index - 1.4 times (P <0.05), the bactericidal activity of blood serum - 1.8 times (P <0.05). «Arcusit» stimulates the synthesis of gamma globulins, increases the opsonophagocytic, bactericidal activity of blood serum. Thus, the analysis of experimental data indicates that the drug «Arcusit» has a pronounced immunostimulating effect, providing the activation of factors of nonspecific resistance of the body of the silver-black fox.

Dynamics of indicators of non-specific pro-tection of holstein black-and-white breeds of cows depending on the month of pregnancy

2020 ◽  
Vol 2 ◽  
pp. 178-182
Author(s):  
L.Y. Karpenko ◽  
◽  
A.A. Pogodaeva ◽  
A.A. Bakhta ◽  
◽  
...  
Keyword(s):  
Natural Resistance ◽  
Control Group ◽  
Correction Factors ◽  
Leningrad Oblast ◽  
Black And White ◽  
Preg Nancy ◽  
Blood Neutrophils ◽  
Group 10 ◽  
The Difference ◽  
Experimental Group

The article discusses the results of the dy-namics of indicators of innate immunity of cattle throughout pregnancy. The research topic is relevant, since receiving healthy offspring is certainly associated with the natural resistance of the cow. The aim of the study is to study the non-specific immunity of cows in different months of pregnan-cy. The work was performed on the farm of JSC Krasnoselskoye PZ (Leningrad Oblast). During the experiment, 2 groups of cows of Holstein black-and-white breeds were formed: the experimental group – 10 preg-nant cows and the control group-10 not steel cows. Blood was taken once a month throughout pregnancy. Indicators of phago-cytosis, bactericidal and lysozyme activities were determined in the blood. During the study, the authors noted a decrease in lyso-zyme, bactericidal activity relative to the indicators of the control group, however, the difference did not reach significant differences. Also observed a sig-nificant inhibition of the functional state of the number of blood neutrophils: a decrease in phagocytic activity reliably observed from 5 months by 27.02%, from 7 month by 41.9% to 9 month, the index decreased by 50,66% as compared to control; at the phag-ocytic number of reliable decrease was ob-served at 4 months 11.3% at 6 months had decreased by 24.86%, and the 9 month preg-nancy reduction made up 28.81%, the phag-ocytic index, starting with the 5th month of pregnancy was significantly decreased by 32.05%, to 8 month decreased by 48.31% and for 9 months had decreased by 50% compared to control. Therefore, in the period of pregnancy in the organism of cows, the development of sec ondary immunodeficiency is noted, which is directly associated with fetal gestation. The data obtained can be useful in correction factors of natural resistance during pregnancy of cows.

scholarly journals Using Biferon-B for the prevention of mastitis in cows

2020 ◽  
Vol 17 ◽  
pp. 00099
Author(s):  
Sergey Shabunin ◽  
Alexander Pashentsev ◽  
Nikolay Klimov ◽  
Valentina Morgunova ◽  
Vasilina Gritsyuk
Keyword(s):  
Nitric Oxide ◽  
Blood Serum ◽  
Immune Complexes ◽  
Catalase Activity ◽  
Phagocytic Activity ◽  
Intramuscular Injection ◽  
Lysozyme Activity ◽  
Blood Levels ◽  
Preventive Effect ◽  
Lactating Cows

The developed method for the prevention of mastitis in lactating cows by intramuscular injection of Biferon-B at doses of 5.0–10.0–20.0 ml, provided a preventive effect in 25.0–75.0 % of animals; the best effect was achieved with Biferon-B at a dose of 5.0 ml. The use of Biferon-B in clinically healthy lactating animals was accompanied by a decrease in blood levels of stab neutrophils by 58.8–65.0 %, circulating immune complexes by 23.4–62.6 %, with a higher content of segmented neutrophils by 4.8–7.8 %, monocytes by 5.6–57.1 %, lymphocytes by 4.9–12.3 %, total immunoglobulins by 5.7–14.3 %, bactericidal and lysozyme activity of blood serum by 6.4–23.1 % and 4.3–13.7 %, respectively. The phagocytic activity of neutrophils decreased by 5.6–10.9 %. With lower indicators of the intoxication index dropped by 12.8–19.1 %, the content of average molecular peptides by 2.4–22.6 %, nitric oxide by 24.5–45.4 %, MDA by 2.8–36.7 %; catalase activity increased by 2.7–12.8 % and GPx by 10.4–29.7 %.

THE EFFECT OF PREBIOTIC HEMIV ON THE NATURAL RESISTANCE AND QUALITY OF MEAT OF BROILER CHICKENS

2021 ◽  
Vol 1 (3) ◽  
pp. 329-334
Author(s):  
A.A. Reznichenko ◽  
◽  
V.I. Dorozhkin ◽  
Keyword(s):  
Body Weight ◽  
Blood Serum ◽  
Meat Quality ◽  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Broiler Chickens ◽  
Natural Resistance

We studied the effect of prebiotic hemiv on natural resistance and meat quality of broiler chickens. As a result of the conducted studies, it was found that after using the drug at a dose of 0,6 g/kg of body weight, there was an increase in the phagocytic activity of pseudoeosinophils and the bactericidal activity of blood serum by 34,3 and 22,4%, respectively, compared with the control. After the use of hemiv at a dose of 0,9 g/kg, there was also a statistically confirmed increase in the bactericidal activity of blood serum and phagocytic activity of pseudoeosinophils by 20,6 and 29,1%. At studying the effect of the drug on the quality of meat, it was found that it belongs to the mature and benign and can be eaten without restrictions.

scholarly journals Constitutional factors of autarcesis of the Orenburg goats

2021 ◽  
Vol 282 ◽  
pp. 03016
Author(s):  
S.V. Nikitina
Keyword(s):  
Blood Serum ◽  
Bactericidal Activity ◽  
White Blood Cells ◽  
Lysozyme Activity ◽  
Lytic Activity ◽  
Humoral Factors ◽  
Group Breeding ◽  
Winter Time ◽  
Summer Time ◽  
Experimental Group

The paper shows the results of a comparative assessment of the seasonal and age patterns of autarcesis in Orenburg goats. Intact healthy goats were studied as control groups: 1st experimental group - breeding male goats (n=10), 2nd group – breeding female goats (n=10), 3rd experimental group - gimmer hogs (n=10), and 4th experimental group - young wethers (n = 10). The autarcesis is marked by humoral factors, for example, indicators of the bactericidal activity of the blood serum, lysozyme activity, and beta-lytic activity (thrombodefensin activity). The investigations have shown that the maximum values of the bactericidal activity of the blood serum were found in the autumn and winter times in the experimental groups. Their values were 73,75±1,07 %, 78,28±0,85 %, 65,89±1,16 %, and 71,16±0,71 %, respectively. The minimum values are determined in spring and summer. A similar pattern was found in the study of beta-lytic activity in the autumn-winter time, but the highest values were observed in the animals of the experimental group of 9.43±0.12% and 9.86±0.15 %, respectively. The decrease in the bactericidal activity of the blood and the beta-lytic activity in the summer time was recompensed by the values of lysozyme activity. Thus, the highest immunological indices were found in summer time. The highest values of white blood cells were found in the blood of the gimmer hogs and young wethers. Meanwhile, the studied indicators did not overstep the limits of the physiological standard. The data obtained are suggested to be used in breeding work for the reproduction of animals with a high level of autarcesis.

scholarly journals C-reactive protein and procalcitonin in biological liquids in men with normal and reduced fertility

2020 ◽  
pp. 68-71
Author(s):  
D. Yu. Sosnin ◽  
K. R. Galkovich ◽  
A. V. Krivtsov
Keyword(s):  
Blood Serum ◽  
Seminal Plasma ◽  
Comparison Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sperm Cells ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Biological Liquids ◽  
And Control ◽  
Experimental Group

Objective: A comparative analysis of C-reactive protein (CRP) and procalcitonin concentration in blood serum and seminal plasma of healthy men and men with reduced concentration of sperm cells.Methods: 73 fertile men were examined. The experimental group included 36 patients with a reduced fertility; the comparison group (control) included 37 men with normal indicators of ejaculate composition. The concentrations of CRP and procalcitonin were determined by the method of enzyme-linked immunosorbent assay.Results: Differences in level of CRP between blood serum and seminal plasma in experimental and control groups occurred to be statistically significant in both groups. There was no correlation between these parameters. The concentration of procalcitonin was almost ten times higher in the seminal plasma than in the blood serum. In the experimen‑ tal group, it was significantly higher in ejaculate than in the comparison group. The concentration of CRP and procalcitonin did not correlate neither with the ejaculate volume nor with concentration or amount of sperm cells in it.Conclusions: The levels of CRP and procalcitonin in seminal plasma can indicate the fertility status of the ejaculate. 

Sign in / Sign up

Export Citation Format

Share Document