scholarly journals KANDUNGAN PIGMEN PADA LAMUN Enhalus acoroides (Linnaeus f.) DI PERAIRAN PANTAI AMADOKE DESA AKLE KECAMATAN SEMAU SELATAN KABUPATEN KUPANG

2021 ◽  
Vol 3 (1) ◽  
pp. 24-31
Author(s):  
Ivin Anggraini Bayang ◽  
Andriani Rafael ◽  
Alfred G.O Kase
Keyword(s):  
Thin Layer ◽  
Marine Ecosystem ◽  
Spectrophotometric Analysis ◽  
Ecological Function ◽  
High Potential ◽  
Pigment Content ◽  
Survey Method ◽  
Research Use ◽  
Shallow Marine ◽  
Enhalus Acoroides

ABSTRAK               Ekosistem lamun merupakan salah satu ekosistem di laut dangkal yang mempunyai peranan penting dalam kehidupan biota laut dan merupakan salah satu ekosistem bahari yang paling produktif, sehingga mampu mendukung potensi sumber daya yang tinggi pula. Fungsi ekologis ekosistem lamun adalah sebagai produsen, pendaur unsur hara, penstabil substrat, penangkap sedimen, habitat dan makanan serta tempat berlindung organisme laut lainnya. Tujuan dari penelitian ini adalah, untuk mengidentifikasi pigmen dan mengetahui kandungan pigmen yang terdeteksi pada lamun Enhalus acoroides (Linnaeus f.) di Perairan Pantai Amadoke Desa Akle Kecamatan Semau Selatan Kabupaten Kupang. Metode yang digunakan dalam penelitian ini adalah metode Survei, Kromotografi Lapis Tipis, dan metode Spektrofotometer. Lamun ini mengandung pigmen terlihat pada hasil analisis spektrofotometer pada ekstrak pigmen total lamun dan pemisahan pigmen lewat uji Kromotografi Lapis Tipis (KLT). Dalam proses ekstraksi digunakan pelarut aseton untuk penggerusan dan perendaman. Dan pada uji spektrofotometer untuk kandungan klorofil a menggunakan panjang gelombang 645 nm dengan pigmen total 226,85 µg/mL, klorofil b 664 nm dengan pigmen total 368,69 µg/mL dan karotenoid 470 nm dengan pigmen total. 2.923,70 µg/mL. Pada pengujian pigmen lewat KLT terdapat sembilan noda yaitu, klorofil a dengan nilai Rf (0,78, 0,84 dan 0,08) klorofil b dengan nilai Rf (0,04),  klorofil c dengan nilai Rf  (0,12), feofitin dengan nilai Rf (0,22 dan 0,88), fukosantin dengan nilai Rf (0,48) dan karotenoid dengan nilai Rf (1,00).      Kata kunci : E. acoroides (Linnaeus f.), pigmen, klorofil dan KLT ABSTRACT Seagress ecosystem is one of the shallow marine ecosystem that has an important role in the life of marine and is one of the most productive marine ecosystem, so that it can support the high potential of high resources as well. The ecological function of seagress ecosystem is as a producer, nutrient recycler, substrat stabilizer, sedimen capture, food and shelter of other organisme. The purpose of the research was to identifi the pigment and determine the pigment content in Enhalus acoroides (Linnaeus f.) seagress in Amadoke waters beach Akle of village, South Semau.The  research use survey method, thin layer chromotographic and spectrophotometer methods. This seagress contains pigment, seen in the result of spectrophotometric analysis in total seagress extract and pigment separation through thin layer chromotography test. In the pisment used acetone for steaming and soaking. The spectrophotometric test for the content of chrolophyll a (λ 645 nm), chrolophyll b (λ 664 nm) and carotenoid (λ 470 nm), respectivelly are 226,85 µg/mL, 368,69 µg/mL and 2923,70 µg/mL. Nine pigments found based on the Rf valuef of TLC analisis are chrolophyll a (Rf 0,78, 0,84, 0,08), chrolophyll b (Rf 0,04), chrolophyll c (Rf 0,12), feofitin (Rf 0,22 dan 0,88) and carotenoid (Rf 1,00)  

Keanekaragaman Perifiton Daun Lamun Enhalus acoroides dan Cymodocea serrulata di Teluk Awur, Jepara

2021 ◽  
Vol 10 (2) ◽  
pp. 306-312
Author(s):  
Adelia Hilma Sugiarto ◽  
Raden Ario ◽  
Ita Riniatsih
Keyword(s):  
Marine Ecosystem ◽  
Survey Method ◽  
Line Transect ◽  
Cross Sectional ◽  
High Productivity ◽  
Enhalus Acoroides ◽  
Seagrass Ecosystem ◽  
Morisita Index ◽  
Cymodocea Serrulata ◽  
Seagrass Density

Ekosistem padang lamun merupakan ekosistem bahari yang memiliki keanekaragaman hayati dan memiliki produktivitas tinggi di perairan dangkal. Adanya keberadaan perifiton yang menempel pada daun lamun diduga dijadikan sebagai faktor penunjang produktivitas primer di kawasan ekosistem lamun. Penelitian ini bertujuan untuk mengetahui kelimpahan perifiton, distribusi perifiton dan hubungan kerapatan lamun terhadap kelimpahan perifiton di Perairan Teluk Awur, Jepara. Penelitian ini menggunakan metode survei dan penentuan lokasi dipilih dengan menggunakan metode sampling purposive method, sedangkan metode pengambilan data lamun mengacu pada metode line transek kuadran. Pengambilan daun lamun untuk pengamatan perifiton menggunakan metode sapuan daun yang selanjutnya diamati dengan menggunakan mikroskop. Nilai kelimpahan perifiton pada daun lamun Enhalus acoroides di Stasiun 1, Stasiun 2, dan Stasiun 3 berturut-turut adalah 105 ind/cm2, 167,5 ind/cm2, dan 101,25 ind/cm2. Sedangkan kelimpahan perifiton pada daun lamun Cymodocea serrulata di Stasiun 1 tidak ada lalu Stasiun 2 dan Stasiun 3 berturut-turut adalah 80 ind/cm2 dan 135 ind/cm2. Kelimpahan tertinggi perifiton terdapat pada jenis lamun E. acoroides diduga karena E. acoroides mempunyai luas penampang daun yang lebih lebar dibandingkan C.serrulata. Perifiton yang mendominasi di lokasi ini berasal dari Kelas Bacillariophyceae. Sebaran perifiton berdasarkan perhitungan indeks morisita yaitu berkelompok dan terdapat hubungan antara kelimpahan perifiton dengan kerapatan lamun.  The seagrass ecosystem is a marine ecosystem that has biodiversity and is high productivity in shallow waters. The presence of periphyton attached to seagrass leaves is thought to be used as a primary productivity supporting factor in the seagrass ecosystem. This research aims to determine the periphyton abundance, periphyton distribution and seagrass density relationship towards periphyton abundance in Teluk Awur, Jepara. The survey method and location determination were selected based on purposive sampling method, while the seagrass data collection method refers to the quadrant line transect method. The taking of seagrass leaf for periphyton observation used leaf drainage method was then observed using a microscope. Periphyton abundance value on seagrass leaves of Enhalus acoroides in Station 1, Station 2, and Station 3 are respectively  105 ind / cm2, 167,5 ind / cm2, and 101, 25 ind / cm2. Periphyton abundance in seagrass leaves Cymodocea serrulata in Station 1 was not found while Station 2 and Station 3 are 80 ind / cm2 and 135 ind / cm2 respectively. The highest abundance of periphyton is in the type of seagrass E. acoroides because E. acoroides has a wider leaf cross-sectional area than C. serrulata. Periphyton that dominates the waters of Teluk Awur comes from the Bacillariophyceae class. Periphyton distribution based on the morisita index calculation is clustered and there is a relationship between periphyton abundance and seagrass density.

scholarly journals Steroidal saponin concentrations in Brachiaria decumbens and B. brizantha at different developmental stages

2008 ◽  
Vol 39 (1) ◽  
pp. 279-281 ◽  
Author(s):  
Karine Bonucielli Brum ◽  
Mitsue Haraguchi ◽  
Mirella Biasoli Garutti ◽  
Fernanda Nogarol Nóbrega ◽  
Beneval Rosa ◽  
...  
Keyword(s):  
Thin Layer ◽  
Developmental Stages ◽  
Ethanolic Extract ◽  
Spectrophotometric Analysis ◽  
Steroidal Saponin ◽  
Steroidal Saponins ◽  
Brachiaria Decumbens ◽  
Aerial Parts ◽  
Seed Fall ◽  
Layer Chromatography

Brachiaria species contain steroidal saponins and are involved in outbreaks of hepatogenous photosensitization. This research presents the levels of a steroidal saponin, protodioscin, in the seeds and aerial parts of B. brizantha and B. decumbens during different developmental stages (growth, bloom, fructification and seed fall). The butanolic fraction of the ethanolic extract of each stage was submitted to thin layer chromatography (TLC) and spectrophotometric analysis through the Ehrlich reagent in 515nm. The chromatograms in TLC of the butanolic fraction of B. brizantha and B. decumbens showed similar spots as the protodioscin standard. The estimated level of protodioscin isomers in B. brizantha and B. decumbens ranged from 0.5% to 2.1%, having the highest level at the end of their developmental stages during seed falling comparison with the previous one. Protodioscin was not detected in the seeds. Outbreaks of Brachiaria spp. poisoning in central Western Brazil are frequently observed in pastures that had been more than 30 days without animals grazing, and also during the growing or blooming stage of the pastures. Other saponin determinations in toxic and non toxic pastures are necessary to determine the saponin concentrations that cause intoxication.

Shelf ecosystem response to the Eocene-Oligocene Transition

2020 ◽  
Author(s):  
Laura Cotton ◽  
David Evans ◽  
Daniela Schmidt
Keyword(s):  
High Resolution ◽  
Environmental Change ◽  
Trace Element ◽  
Marine Ecosystem ◽  
Marine Ecosystems ◽  
Open Ocean ◽  
Ecosystem Dynamics ◽  
Ecosystem Response ◽  
Holistic View ◽  
Shallow Marine

<p>The Eocene-Oligocene transition (EOT) is one of the most dramatic climate shifts of the Cenozoic with severe consequences for reef ecosystems. The onset of continental Antarctic glaciation is associated with widespread environmental change, resulting in a global peak in biotic turnover. Whilst numerous studies of the biotic response to the changes at the EOT have been carried out, most high-resolution studies consist of open ocean records of marine plankton and predominantly single groups of organisms. However, this is not representative of the ocean system as a whole and does not provide a holistic view of mechanism of restructuring of the marine ecosystems. The shelf seas and reefs are some of the most diverse and fundamentally important ecosystems of the oceans. Long-term diversity loss across the EOT has been shown in several macrofossil studies, but mainly at low resolution, and recovery is not well understood.  Many shelf species are ecosystem engineers whose loss and recovery have profound implications for the entire ecosystem. Understanding these interactions will provide insights into shallow marine ecosystems and their response to major climate perturbations.</p><p>The Tanzanian Drilling Project EOT record (TDP 11, 12, 17) is recognised globally for its completeness and exceptionally preserved calcareous microfossils. It is most importantly, though, a rare record of both shallow water organisms and open ocean plankton. The latter are fundamentally important for reconstructions of the environment and a globally calibrated timescale. Here we draw together a unique dataset of high-resolution mollusc, Dasycladaceae, bryozoan, larger benthic foraminifers, coral, smaller benthic foraminifera, trace element and isotope records from the EOT. The response and recovery of these species is compared with known, modern physiology of each group to provide a complete picture of the shallow marine ecosystem response.</p><p>Following rapid extinctions within the larger foraminifera during the transition, molluscs, Dasycladaceae and bryozoans all show increases in abundance, indicating a major shift in shelf ecosystem composition. These assemblage changes are coincident with a period of more positive values in d<sup>13</sup>C of both benthic and planktonic foraminifera and changes in trace element values. Comparison with the open ocean record of planktonic foraminiferal, pteropod, and nannofossils confirm these assemblage changes are a biological, rather than sedimentological response and additionally support a that a transition to more eutrophic conditions took place. an environmental framework of traditional and novel geochemistry, indicate that increased nutrient fluxes played a pivotal role in restructuring shelf ecosystem dynamics and therefore offers new insight into mechanisms of reorganisation under ecosystem loss and environmental change.</p>

scholarly journals FACTORS INFLUENCING EMPLOYEES TO COMMIT FRAUD IN WORKPLACE EMPIRICAL STUDY IN INDONESIAN HOSPITALS

2017 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Aditya Pandu Wicaksono ◽  
Dekar Urumsah
Keyword(s):  
Unethical Behavior ◽  
Religious Faith ◽  
Survey Method ◽  
Research Use ◽  
Financial Factors ◽  
Factors Influencing ◽  
Love Of Money ◽  
Negative Effect ◽  
Convenience Sampling ◽  
Positive Effect

Fraud is a part of unethical behavior, which occurs around the world. It occurs because of some factors. This study aims to analyze the factors influencing fraud intention. This research use survey method by distribute questionnaire to respondent with convenience sampling to collect data. The questionnaires fill completely by 154 non-medical employees in some hospitals in Yogyakarta, Indonesia. Smart PLS 2.0 is used to analyze the statistics. The result of the study indicates that organizational commitment and morality leadership have positive effect against fraud intention. Meanwhile, love of money, morality leadership, work environment, religious faith, and organizational culture has negative effect against fraud intention. This research provides the influence of financial and non-financial factors to fraud intention and results can be guideline to protect organization from unethical act.

scholarly journals Shallow marine ecosystem collapse and recovery during the Paleocene-Eocene Thermal Maximum

2021 ◽  
Vol 207 ◽  
pp. 103649
Author(s):  
Skye Yunshu Tian ◽  
Moriaki Yasuhara ◽  
Huai-Hsuan M. Huang ◽  
Fabien L. Condamine ◽  
Marci M. Robinson
Keyword(s):  
Marine Ecosystem ◽  
Shallow Marine ◽  
Thermal Maximum ◽  
Ecosystem Collapse

scholarly journals Isolasi dan Karakterisasi Bakteri Proteolitik yang Berasosiasi dengan Lamun Enhalus acoroides di Pantai Bama, Taman Nasional Baluran, Situbondo, Jawa Timur [Isolation and Characterization Proteolytic Bacteria which is Associated with Sea Grass (Enhalus acoroides) in Bama Beach, Baluran National Park, Situbondo, East Java].

2018 ◽  
Vol 10 (1) ◽  
pp. 8 ◽  
Author(s):  
Rachmat Rizaldi ◽  
Woro Hastuti Setyantini ◽  
Sudarno Sudarno
Keyword(s):  
National Park ◽  
Sea Water ◽  
Skim Milk ◽  
Survey Method ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Enhalus Acoroides ◽  
Isolation And Characterization ◽  
Protease Enzyme ◽  
Proteolytic Bacteria

AbstrakLamun adalah tumbuhan sejati yang hidup di perairan pantai yang kurang dimanfaatkan dalam bidang perikanan, selain sebagai bioindikator kualitas air laut. Beberapa mikroorganisme yang berasosiasi dengan lamun Enhalus acoroides antara lain benthos, kapang, bakteri dan plankton. Bakteri proteolitik merupakan bakteri yang mampu menghasilkan enzim protease. Enzim perotese merupakan enzim yang paling banyak digunakan dalam kehidupan. Bakteri merupakan sumber enzim yang paling banyak digunakan dibandingkan dengan tanaman dan hewan. Tujuan penelitian ini adalah untuk mengetahui jenis isolat bakteri proteolitik yang berasosiasi dengan lamun Enhalus acoroides di Taman Nasional Baluran, Situbondo. Penelitian ini menggunakan metode survei dengan dianalisis secara deskriptif dengan bantuan tabel dan gambar. Hasil yang peroleh menunjukkan bahwa terdapat 12 isolat bakteri yang berasosisasi dengan lamun Enhalus acoroides. Terdapat empat isolat yang tergolong sebagai bakteri proteolitik karena mampu mendagradasi kasein dalam media TSA + 2% NaCL yang ditambah 1 % susu skim, tampak dari pembentukan zona bening. Pengamatan morfologi koloni dan sel serta pengujian biokimia dari keempat isolat (EA-1, EA-2, EA-9 dan EA-10) terdapat kesamaan karakteristik dengan empat genus bakteri berturut-turut yaitu Staphylococcus sp., Plesiomonas shigeloides, Bacillus sp., Pseudomonas sp.AbstractSeagrass is a true living plants underutilized coastal waters in the field of fisheries, as well as bio-indicators of the quality of sea water. Some microorganisms associated with seagrass Enhalus acoroides among others benthos, fungi, bacteria and plankton. Proteolytic bacteria are bacteria that are capable of producing the enzyme protease. Protease enzyme is an enzyme that is most widely used in life. Bacteria are a source of enzymes that are most widely used compared to plants and animals. The purpose of this study was to determine the type of proteolytic bacterial isolates associated with seagrass Enhalus acoroides in Baluran National Park, Situbondo. This study used survey method with descriptive analysis with tables and figures. The results obtained that there are 12 bacterial isolates associated with seagrass Enhalus acoroides. There are four isolates were classified as proteolytic bacteria because it can degrade casein in TSA media + 2 % NaCL plus 1% skim milk which is evidenced by the formation of clear zones. Observations colony morphology and cells, as well as testing of Biochemistry of the four isolates (EA-1, EA-2, EA-9 and EA-10), were obtained, with similar characteristics to the four genera of bacteria in a row as follows Staphylococcus sp., Plesiomonas shigeloides, Bacillus sp., Pseudomonas sp. 

scholarly journals ANALISIS FAKTOR-FAKTOR YANG MEMPENGARUHI KESEJAHTERAAN NELAYAN DI KABUPATEN JEMBRANA

2018 ◽  
pp. 2437
Author(s):  
Dwi Kusuma ◽  
I Nyoman Djinar Setiawina ◽  
I Made Suyana Utama
Keyword(s):  
Social Capital ◽  
Data Analysis ◽  
Statistical Data ◽  
Survey Method ◽  
Research Use ◽  
Statistical Data Analysis ◽  
Production Factors ◽  
Factors Of Production ◽  
Positive Effect

This study aims to analyze the influence of social capital on income, analyze the factors of production to income, analyze the influence of social capital to the welfare of fishermen, analyze the influence of production factors to fisherman's welfare in Jembrana regency, and analyze the influence of social capital and production factors through income mediator to fisherman's welfare in Jembrana District. In this research use survey method by taking sample 98 responden from population of 4,876 people, with technique of descriptive statistical data analysis and analysis of SEM PLS. The results of testing in the study showed: 1) Social capital positively affects the income of fishermen; Factor of Production have positive effect to fisherman's income; 2) Social capital has a positive effect on fishermen's welfare in Jembrana Regency; Factor of Production have positive effect to fisherman's prosperity in Jembrana Regency; 3) Revenues mediate the influence of social capital and production factors on fishermen's welfare in Jembrana District.

Changes in Epicuticular Flavonoids and Photosynthetic Pigments as a Plant Response to UV-B Radiation

1996 ◽  
Vol 51 (9-10) ◽  
pp. 671-680 ◽  
Author(s):  
Pedro Cuadra ◽  
Jeffrey B. Harborne
Keyword(s):  
Plant Growth ◽  
Photosynthetic Pigments ◽  
Hplc Analysis ◽  
Leaf Surface ◽  
Spectrophotometric Analysis ◽  
Plant Response ◽  
Pigment Content ◽  
Plant Chemistry

Treatment of Gnaphalium vira-vira plants with UV-B radiation caused changes in plant growth and in plant chemistry. The leaf surface contained two O-methylated flavones, araneol and 7-O-methylaraneol. HPLC analysis showed that 20 days of UV-B radiation increased the synthesis of 7-O-methylaraneol at the expense of araneol. Spectrophotometric analysis of the photosynthetic pigments showed that UV-B radiation also increases the pigment content in treated plants. Another U V alteration is epidermal hair damage, as observed in SEM pictures of treated leaves. This combination of physiological and phytochemical effects may be interpreted as a plant response to UV-B stress

Use of isotopic signatures to assess the food web in a tropical shallow marine ecosystem of Southeastern Brazil

2006 ◽  
Vol 40 (3) ◽  
pp. 381-390 ◽  
Author(s):  
T.N. Corbisier ◽  
L.S.H. Soares ◽  
M.A.V. Petti ◽  
E.Y. Muto ◽  
M.H.C. Silva ◽  
...  
Keyword(s):  
Food Web ◽  
Marine Ecosystem ◽  
Southeastern Brazil ◽  
Shallow Marine ◽  
Isotopic Signatures

scholarly journals Hubungan antara Komunikasi Interpersonal dengan Kinerja Guru SMK Swasta di Kecamatan Bogor Utara Kota Bogor

2017 ◽  
Vol 4 (1) ◽  
pp. 19
Author(s):  
Wisnu Diputra ◽  
Neti Karnati ◽  
Matin Matin
Keyword(s):  
High School ◽  
Interpersonal Communication ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Survey Method ◽  
Research Use ◽  
Vocational High School ◽  
Positive Relation ◽  
Independent Variable ◽  
R Value

This study aims to know there is a relationship between headmastersinterpersonal communication with teachers performance at Vocational High SchoolNorth Bogor District, Bogor City. This research use quantitative with two variables therein, namely: (X) interpersonal communication as independent variable and (Y) teachersperformance as the dependent variable. The method used is survey method with theresults of the analysis of correlational studies. The population in this study are teachersat vocational high school in North Bogor District, Bogor City, there are 580 teachers.Samples were taken by using simple random sampling technique. The technique is basedon the sample size used in this study as many as 85 teachers. Data collection for thevariable (X) interpersonal communication and variable (Y) teachers performance using aquestionnaire. Hypothesis testing is done by using product moment correlation formulaand getting the correlation coefficient (r) value = 0,58892 and based on the results ofhypothesis testing by using t-test obtained t for 6,639 ˃ 1,671 t-tables. By this research,we get a positive relation between interpersonal communication with teachersperformance at Vocational High School North Bogor District, Bogor City.

Sign in / Sign up

Export Citation Format

Share Document