scholarly journals CONTROL OF RADIOACTIVE SAFETY AND QUALITY OF MEDICINAL VEGETABLE RAW MATERIALS OF THE GREATER BURDOCK (ARCTIUM LAPPA L.) ROOTS IN VORONEZH REGION

2019 ◽  
Vol 8 (1) ◽  
pp. 73-77
Author(s):  
N. A. Dyakova ◽  
A. I. Slivkin ◽  
S. P. Gaponov
Keyword(s):  
Raw Materials ◽  
Surrounding Medium ◽  
Correlation Coefficients ◽  
Water Soluble ◽  
Raw Material ◽  
Bioactive Substances ◽  
Anthropogenic Effect ◽  
Perennial Plant ◽  
Voronezh Region ◽  
A Minor

Introduction. Radionuclides are recognized as one of the most dangerous pollutants of a surrounding medium in forces of their fissile migration on biological chains and influences on metabolism of alive organisms now. Aim. Studying of accumulation of radionuclides and polysaccharides in roots of a burdock big, collected in the territory of the Voronezh region in the areas which are influenced various anthropogenic influence and also an interrelation research between accumulation of these groups of substances. Materials and methods. The raw material was collected in the territory of Voronezh Region in the areas with different anthropogenic impact. Based on cartography the 36 points of the medicinal vegetable raw material samples collection were chosen in Voronezh Region. The subject matter was the roots of the Greater Burdocks as a wide spread perennial plant. The main artificial (Sr-90 and Cs-137) and natural (K-40, Th-232 and Ra-226) radionuclides as well as a concentration of the total amount of polysaccharides in terms of fructose and the total amount of gravimetrically determined polysaccharides were defined in the samples. Results and discussion. All the samples met the requirements of the pharmacoeial article of the artificial radionuclides content. A content of the total amount of polysaccharides in terms of fructose determined by spectrophotometry significantly varied in the root samples depends on their collection point. Thus, in protected reserved areas a content of the total amount of polysaccharides in term of fructose was 30-45 % high than in the samples from ecologically unfavourable areas. However all the samples also met the requirements of the pharmacoeial article of the total amount of polysaccharides in terms of fructose. A content of the polycaccharides close to the lower norm was found out in a sample collected in Povorino, while a content of the polycaccharides in terms of fructose significantly exceeded the normative standard in the rest of the samples. A content of gravimetrically determined water-soluble polysaccharides in the roots varied from 21,17 to 37,82 %. No noticeable anthropogenic effect on the accumulation of these substances was found. The samples from ecologically unfavourable and protected areas were little different from each other in a quantitative content of water-soluble polysaccharides. Conclusion. The correlation coefficients between the content of radionuclides and major groups of bioactive substances in the roots indicated that the radionuclides accumulated in the greater budock roots in a minor amounts and they did not affect the content of bioactive substances in plant raw material.

scholarly journals Accumulation of Reducing Sugars by Small-leaved Linden Flowers (Tilia cordata Miller, 1768) in the Voronezh Region of Russia

2021 ◽  
Vol 10 (4) ◽  
pp. 147-153
Author(s):  
N. A. Dyakova ◽  
A. I. Slivkin ◽  
S. P. Gaponov ◽  
E. A. Bobina ◽  
L. A. Shishorina
Keyword(s):  
Heavy Metals ◽  
Protected Areas ◽  
Reducing Sugars ◽  
Raw Materials ◽  
Correlation Coefficients ◽  
Reducing Sugar ◽  
Water Soluble ◽  
Anthropogenic Factors ◽  
Voronezh Region ◽  
Sugar Levels

Introduction. Small-leaved linden flowers are mainly used for aquatic extracts, and their pharmacological benefit is based on water-soluble polysaccharides.Aim. The aim of this study was to investigate the rate of accumulating all reducing sugars in small-leaved linden flowers, collected in agro- and urbo-cenoses of the Voronezh region.Materials and methods. 51 sites were selected for collecting flowers from the small-leaved linden, which is a widespread deciduous tree species in Russia. In order to determine the total level of reducing sugars in the samples, we measured glucose levels using the method described in Pharmacopeia article "Linden Flower". Correlation coefficients were analyzed to examine in detail the effect of the major pollutants (heavy metals and arsenic) on the accumulation of reducing sugars in small-leaved linden flowers.Results and discussion. All analyzed samples of medicinal plant raw materials were benign in terms of their reducing sugar levels. Samples collected in control (protected) areas contained reducing sugar levels 13.31 to 16.89 %, which is 6–8 times more than the lower numerical value established by the Pharmacopoeia article. In the agrocenoses of the region, the concentration of reducing sugars varied from 6.12 to 16.68 %, which is 3–8 times more than the value given in the Pharmacopoeia article. In the urbocenoses of the region, a lower level of reducing sugars was found compared to samples from protected areas (2.35–13.49 %). Correlation coefficients showed a noticeable negative impact of cadmium, chromium, cobalt, copper, zinc, as well as a moderate negative effect of lead and mercury on the accumulation of reducing sugars in the small-leaved linden flowers.Conclusion. The lowest concentrations of reducing sugars were detected in samples harvested along the streets of large cities in the region, and along highways, roads and railways. This suggests that anthropogenic factors might negatively impact the accumulation of reducing sugars in small-leaved linden flowers in these areas. At the same time, it is possible that saccharide complexes might have reacted with heavy metals instead of the complexing agent when levels of reducing sugars in the samples were quantitatively determined. This would have the effect of underrepresenting reducing sugar levels in the raw materials.

scholarly journals Useful Bioactive Substances from Wastes: Recovery of Trans-Resveratrol from Grapevine Stems

2016 ◽  
Vol 10 (1) ◽  
pp. 4-9 ◽  
Author(s):  
George Angelov ◽  
Lubomir Boyadzhiev ◽  
Silviya Georgieva
Keyword(s):  
Water Solubility ◽  
Pharmaceutical Preparations ◽  
Water Soluble ◽  
Raw Material ◽  
Bioactive Substances ◽  
Total Extract ◽  
Initial Extract ◽  
Efficient Extraction ◽  
Simple Extraction ◽  
The Many

The methods for producing natural resveratrol are of big interest because of the many health benefits of this substance and its increasing use in functional foods, food supplements and para-pharmaceutical preparations. Generally, resveratrol is extracted from different natural sources, most of them usually produced for consumption purposes (grapes, nuts). This paper presents a method for recovery of resveratrol from a widely available raw material - grapevine stems, a by-product of vine pruning. An efficient extraction-fractionation scheme is developed, based on shifting the phase equilibrium, by which more concentrated extracts of resveratrol are obtained. After a simple extraction, the initial extract is further separated into two fractions, containing either water or ethanol-soluble compounds. Using this approach, the resveratrol’s low water solubility helps isolate it from other water-soluble substances. The resulting product is almost ten times more concentrated in trans-resveratrol than the initial total extract. Additionally, a fraction containing water-soluble polyphenols is obtained, which could be used for water-based pharmaceutical and cosmetic preparations.

STUDY OF PROTEIN FRACTIONS OF HIGH-TEMPERATURE HYDROLYZATES OBTAINED FROM SMOKED SPRAT HEADS

Fisheries ◽  
2020 ◽  
Vol 2020 (2) ◽  
pp. 113-117
Author(s):  
Olga Mezenova ◽  
Vladimir Wolkov ◽  
Larisa Baydalinova ◽  
Natalia Mezenova ◽  
Svetlana Agafonova ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Chemical Composition ◽  
Raw Materials ◽  
Weight Fraction ◽  
Water Soluble ◽  
Protein Fractions ◽  
Raw Material ◽  
Organoleptic Characteristics ◽  
Pre Treatment ◽  
Peptide Fractions

The authors study three fractions obtained as a result of hydrolysis of smoked sprat heads (under temperature of 130oC and presser of 0.25 MPa) – fat, protein water-soluble, and protein-and-mineral ones. Waste from sprat production of two fish canning complexes of the Kaliningrad Region - “RosCon” and “Kolkhoz for the Motherland” - was used as raw material. Hydrolysis was carried out in an aqueous medium in two ways - with preliminary separation of fat and without this operation. The protein fraction was sublimated and its quantitative and qualitative indices were examined - mass yield, solubility, chemical composition and molecular fractional composition of the obtained peptide fractions were determined. The output of sublimated protein fractions is practically independent of the type of raw material and the method of pre-treatment and is 6.47.9% of the mass of raw materials. The chemical composition of protein fractions varies widely in terms of fat (1.4–8.3%), minerals (9.8–13.4%) and proteins (72.1–80.2%). The solubility of the peptide fractions ranged from 91-98%. The molecular weight assessment results showed a high content of a low molecular weight fraction of peptides with an MM of less than 10 kDa in all experimental samples (about 38%). This indicates a high digestibility and biological value of the obtained peptide compositions. Sublimated peptide compositions had typical organoleptic characteristics, pleasant aroma and taste of smoked fish. Ключевые

scholarly journals Experimental Determination of Biologically Active Compounds in Pomace of Siberian Beet and Carrot

2021 ◽  
Vol 51 (1) ◽  
pp. 179-187
Author(s):  
Anastasia Kozhemayko ◽  
Irina Sergeeva ◽  
Irina Dolgolyuk
Keyword(s):  
Functional Food ◽  
Nutritional Value ◽  
Raw Materials ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Positive Trend ◽  
Bioactive Substances ◽  
Industrial Processing

Introduction. With the development of the food and processing industry, the matter of environmental pollution is becoming more and more acute. Environmental protection is based on the principle of rational use of natural resources and sustainable technology. Vegetable pomace is a secondary raw material; its amount depends on the production technology and equipment. The observed positive trend in the gross harvest of vegetables in open ground can increase the number of vegetable processing enterprises and the capacity of existing enterprises. Eventually, waste will start accumulating at processing sites, and it will have to be used as raw materials. The present paper features the content of biologically active substances in pomace of carrots and beets grown on the territory of the Siberian region and introduces options for their further use in functional foods. Study objects and methods. The research featured carrot pomace of the varieties Losinoostrovskaya, Nantskaya, and Queen of Autumn, as well as beet pomace of varieties Cylinder and Bordeaux. All the samples were harvested in the Kemerovo region in 2019. Determination of physical and chemical parameters was carried out using standard methods. Carotenoids, flavonoids, β-cyanine were studied using spectrometry and photocolorimetric method. Results and discussion. The experiment featured the content of bioactive substances in pomace of carrots and beets obtained during industrial processing. The content of carotenoids in carrots (mg of β-carotene per 100 g of dry weight): for Losinoostrovskaya variety – 23.56 ± 0.23; Nantskaya – 25.32 ± 0.18; Queen of Autumn – 20.78 ± 0.25. Flavonoid content (mg of catechol equivalent per 100 g of dry weight): Losinoostrovskaya – 12.02 ± 0.37; Nantskaya – 13.45 ± 0.56; Queen of Autumn – 11.50 ± 0.48. The content of β-cyanine in beets (mg per 100 g of dry weight): Cylinder – 100.0 ± 8.5; Bordeaux – 35.0 ± 1.8. The nutritional value of carrot and beet pomace with a mass fraction of moisture was 10%. The nutritional value of vegetable pomace is due to the high content of dietary fiber; therefore, the raw materials can be considered for functional food production. The content of biologically active substances in vegetable pomace (flavonoids, carotenoids, β-cyanines) can enhance the functional orientation of this secondary raw material when used in food technologies for the production of food of high nutritional value. Conclusion. The results obtained will make it possible to use the biochemical potential of plant raw materials in many aspects, as well as to obtain new functional food products, thereby expanding the range of healthy foods.

scholarly journals Accumulation of heavy metals and arsenic with medicinal herbal raw material of common shovel harvested in Voronezh region

2021 ◽  
Vol 21 (4) ◽  
pp. 478-487
Author(s):  
Nina A. Dyakova ◽  
Keyword(s):  
Heavy Metals ◽  
Crop Production ◽  
Raw Materials ◽  
Anthropogenic Pollution ◽  
Raw Material ◽  
Cobalt Chromium ◽  
Active Centers ◽  
Plant Raw Materials ◽  
Nickel Copper ◽  
Voronezh Region

The Voronezh region is traditionally the most important area of crop production and agriculture. The purpose of the research was to study the contamination with heavy metals of medicinal plant raw materials of the Voronezh region using the example of the roots of ordinary burdock, prepared in urbo- and agro-ecosystems, which have various anthropogenic effects on themselves. The accumulation of heavy metals (lead, mercury, cadmium, nickel, copper, zinc, cobalt, chromium) and arsenic in 51 samples of raw materials was studied. By comparing the heavy metal content in the upper soil layers of the region and the content of these elements in the roots of the bladder, it can be assumed that there are significant physiological barriers to the accumulation of ecotoxicants in the roots of the bladder, which is especially noticeable for elements such as lead, mercury, arsenic, cadmium, cobalt and chromium. At the same time, this type of medicinal vegetal raw material is able to selectively concentrate some heavy metals included in the active centers of enzyme systems (copper and zinc). Thus, for an ordinary bladder under conditions of anthropogenic load, an edaphotype is formed, which is as a result of selection in conditions of anthropogenic pollution of the external environment and the manifestation of adaptation to these conditions.

scholarly journals IMPROVING THE METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE RAW MATERIAL “ROSEHIP FRUIT”

2020 ◽  
pp. 131-138
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Ol'ga Vladimirovna Sharova ◽  
Polina Valeriyevna Afanaseva
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Water Soluble ◽  
Biologically Active ◽  
Raw Material ◽  
Test Quality ◽  
Total Carotenoids ◽  
Long Time

The fruits of wild rose (Fructus Rosae) have been widely used in both traditional and officinal medicine for the long time. The pharmacological effect of the Rosehip medicine is related to the content of such biologically active compounds as: water-soluble (ascorbic acid) and fat-soluble (carotenoids) vitamins, as well as flavonoids (astragalin, isoquercitrin, tiliroside). Such medicine as Holosas, Carotolin and Rosehip syrup are obtained from the fruits of this plant. In addition, wild rose fruits are part of the various fees from which patients could prepare water infusions at home themselves. Regulatory documentation provides for the analysis of Rosehip raw materials three parameters: the content of ascorbic acid, the total carotenoids and flavonoids. The quantitative content of the total carotenoids in the fruits of the rosehip is regulated for the production of rosehip oil and Carotolin. However, in our opinion, the method of quantitative determination of the total carotenoids, described in the State Pharmacopoeia of the Russian Federation of the XIV edition, is too complicated and contains an unreasonably entered coefficient in the formula, leading to an overestimation of the analysis results in 10 times. We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30. Investigated solution there was obtained by diluting the hexane extract in the ratio of 2:25 and measured the optical density at wavelength 450 nm. Adjustments have been made to the formula for the calculating of the total carotenoids relating to the removal of the coefficient 10 unjustifiably entered into the pharmacopoeial methodic. It was determined that the content of the total carotenoids calculated on ß-carotene varied from 10.15 to 74.5 mg%. The metrological characteristics of the methodic indicate that the error of a single determination of the total carotenoids in the rosehip fruits with a 95% confidence level is ± 3.57%. Based on the results of the studies, a new test quality indicators “content of the total carotenoids at least 30 mg%” was substantiated instead of 300 mg%.

scholarly journals DEVELOPMENT AND VALIDATION OF EXPRESS PROCEDURE FOR ISOLATION AND QUANTITATIVE DE-TERMINATION OF WATER-SOLUBLE POLYSACCHARIDES FROM HIGH DEVISIL ROOTS

2020 ◽  
Author(s):  
Nina Alekseyevna Dyakova ◽  
Sergey Petrovich Gaponov ◽  
Aleksey Ivanovich Slivkin
Keyword(s):  
Raw Materials ◽  
Water Soluble ◽  
Biologically Active ◽  
Raw Material ◽  
Ultrasound Frequency ◽  
Treatment Conditions ◽  
Sediment Mass ◽  
Development And Validation ◽  
Active Substances

The aim of the study was to develop and validate an express procedure for the isolation and quantification of water-soluble polysaccharides from high devisyl roots (Ínula helenium L.). In order to accelerate the process of extracting biologically active substances from the roots of high devisil, as well as to increase the yield of water-soluble polysaccharides, it was decided to use an ultrasonic bath. By varying the process parameters, it was possible to select optimal extraction conditions of water-soluble polysaccharides of high-grade roots under ultrasound treatment conditions: raw material grinding 0.5–1.0 mm, temperature – 80 °C, extraction multiplicity – 3, extraction duration – 15 min, ultrasound frequency – 35 kHz, ratio of raw material to extractor 1 g per 15 ml. The proposed technique allows to intensify the process of preparing polysaccharides water-soluble from roots high and to reduce the time spent on it up to 4-5 hours, as well as to increase the yield of the product up to 31.6% in terms of absolutely dry raw materials. The developed method is precise under the conditions of repeatability, correct, stable and has a rather strict linear dependence of the sediment mass on the analysed raw material mass at gravimetric determination of polysaccharides water-soluble in the roots of high devisil. The technique can be used for express analysis of high devasil roots quality, as well as for industrial production of water-soluble polysaccharides from this kind of raw material.

scholarly journals Chemical and bioactive properties of the oils from Brazilian nuts

2020 ◽  
Vol 19 (2) ◽  
pp. 180-186
Author(s):  
Tainara Costa ◽  
Carolina Médici Veronezi ◽  
Neuza Jorge
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Raw Materials ◽  
Raw Material ◽  
Total Carbohydrate ◽  
Total Carotenoids ◽  
Bioactive Substances ◽  
Lipid Fractions

This paper aims to determine the content of bioactive substances of lipid fractions extracted from Bertholletia excelsa, Lecythis pisonis and Dipteryx lacunifera, in by the interest of better identifying the quality of these raw materials. Proximate composition of nuts was determined by official methods and total carbohydrate was calculated by difference. The oils were extracted from the nuts by cold pressing and analyzed for fatty acid composition, tocopherols, phytosterols and total carotenoids and phenolics compounds. The fatty acid composition of oil extracted from L. pisonis was more unsaturated compared with others oils. L. pisonis oil showed to be richer in total tocopherol and γ-tocopherol, moreover showed considerable total phytosterol and carotenoid amounts, thus representing an important source of vitamins A and E. The oils showed significant content of phenolic compounds, with the exception of the oil extracted from D. lacunifera. The study revealed that the oils from Brazilian nuts contain bioactive compounds in relevant quantities, L. pisonis oil standing out. This fact favors their use for food and, as raw material in chemical, pharmaceutical and cosmetic industries, adding value to products derived from these oils, increasing the viable sources of raw materials.

scholarly journals TO THE QUESTION OF JUSTIFICATION OF RATIONAL CONDITIONS OF WAX RAW MATERIAL PURIFICATION IN WATER WITH INTENSIVE MECHANICAL MIXING

2020 ◽  
Author(s):  
Д.Е. Каширин ◽  
И.А. Успенский ◽  
В.В. Павлов ◽  
И.А. Юхин ◽  
А.А. Петухов
Keyword(s):  
Raw Materials ◽  
Heat Exposure ◽  
Bound State ◽  
Physical Principle ◽  
Water Soluble ◽  
Raw Material ◽  
Mechanical Mixing ◽  
Mechanical Stirring ◽  
Organic Impurities ◽  
Short Time

лавной технологической операцией при производстве пасечного воска является его термическое выделение из воскового сырья различной сортности с применением сухого или влажного способа вытопки, реализуемого посредством воскотопок всевозможных типов и конструкций. Так как в основу процесса вытопки положен физический принцип стекания расплавленного воска, сырье подвергается обязательному тепловому воздействию с переходом содержащегося в нем свободного воска в жидкое состояние. Сортность воскового сырья непосредственно связана с его восковитостью – массовой долей воска и зависит от количества и состава содержащихся в нем загрязнений. Расплавленный воск взаимодейсвтует с загрязняющими примесями, впитывается в них, переходя в связанное состояние, при этом выход свободно стекаемого воска уменьшается. В основу предлагаемого способа очистки воскового сырья от перги и других водорастворимых примесей положено удаление загрязнений из предварительно измельченных сотов до их горячей переработки путем погружения загрязненного вороха в емкость с водой и интенсивного механического перемешивания в течение непродолжительного времени, в результате чего органические примеси диспергируются или переходят в раствор, и появляется возможность отделить их от очищенных восковых частиц путем процеживания. Целью данного исследования является установление зависимости количества удаленных загрязнений из воскового сырья в результате его очистки в воде при механическом перемешивании лопастной мешалкой с электроприводом от интенсивности процесса перемешивания и его продолжительности. В результате проведенного исследования получена регрессионная модель процесса и проведена оптимизация с установлением максимального значения критерия и соответствущих ему значений исследуемых факторов. The main technological operation in the production of apiary wax is its thermal separation from wax raw materials of various grades using a dry or wet refining method, realized through wax reflows of various types and designs. Since the basis of the flooding process is the physical principle of runoff of molten wax, the raw material is subjected to mandatory heat exposure with the transition of the free wax contained in it into a liquid state. The grade of the wax raw material is directly related to its waxiness - the mass fraction of wax and depends on the amount and composition of the impurities contained in it. The molten wax interacts with contaminants, is absorbed into them, passing into a bound state, while the yield of freely draining wax decreases. The basis of the proposed method for cleaning wax raw materials from bee bread and other water-soluble impurities is the removal of contaminants from pre-ground honeycombs before hot processing by immersing the contaminated heap in a container with water and intensive mechanical stirring for a short time, as a result of which organic impurities are dispersed or transferred to solution, and it becomes possible to separate them from the purified wax particles by filtering. The aim of this study is to establish the dependence of the number of removed contaminants from wax raw materials as a result of their purification in water with mechanical stirring with a paddle mixer with an electric drive on the intensity of the mixing process and its duration. As a result of the study, a regression model of the process was obtained and optimization was carried out with the establishment of the maximum value of the criterion and the corresponding values ​​of the studied factors.

Effect of Carbohydrate Substrate from Starch-Containing Raw Materials on the Composition of Glucans in the Micromycete Aspergillus niger VKPM F-171 Biomass

2020 ◽  
Vol 36 (5) ◽  
pp. 41-53
Author(s):  
B.S. Manzhieva ◽  
A.A. Printseva ◽  
T.V. Vybornova ◽  
A.S. Miroshnik ◽  
I.V. Kruchina-Bogdanov ◽  
...  
Keyword(s):  
Microbial Biomass ◽  
Corn Starch ◽  
Raw Materials ◽  
Fold Increase ◽  
Water Soluble ◽  
Raw Material ◽  
Soluble Carbohydrates ◽  
Gas Liquid Chromatography ◽  
Whole Grain ◽  
Whole Grain Flour

The conditions for biocatalytic cleavage of corn starch and rye whole grain flour for the subsequent cultivation of the Asperillus niger strain VKPM F-171 are described. As a result of this procedure, the amount of water-soluble carbohydrates in the substrates increased by 5-6 times in comparison with the initial raw material, and the content of glucan p-form in the hydrolysate of rye whole grain flour grew by 2-3 times. The content of glucans in the biomass by the end of the cultivation of A. niger VKPM F-171 on corn starch hydrolysate was 28±2% of dry matter, while the proportion of glucans after fermentation on the rye whole grain flour hydrolysate was 21±1%, 96-97% of which were β-glucans. The hydrolysis with Trichoderma longibrachiatum β-glucanase and Trichoderma reesei xylanase resulted in a 1.1-1.2-fold increase in the proportion of soluble carbohydrates in microbial biomass. It was found via gas-liquid chromatography that the structural units of carbohydrates in microbial biomass are represented by glucose and its 2- and 6-deoxy derivatives, fructose, fucose, galactose, mannose, xylose, and, to the greatest extent, by the reserve saccharide sedoheptulose. It was established that β-glucans are products of hydrolysis of the chitin-glucan complex and glycopeptides. The molecular weight of the obtained microbial glucan-containing biopolymers ranges from 0.5 to 50 kDa. It was shown that the content of β-glucans in microbial biomass allows the latter to be considered as an alternative to plant sources. biosynthesis, Asperillus niger, biocatalysis, starch-containing raw materials, glucans

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