scholarly journals ANTIFUNGAL, ANTIBACTERIAL AND ANTIOXIDANT COMPONENTS OF ETHYL ACETATE EXTRACT OF QUINOA STEM

2019 ◽  
Vol 3 (3) ◽  
pp. 125-130
Author(s):  
Iqra Haider Khan ◽  
Arshad Javaid
Keyword(s):  
Ethyl Acetate ◽  
Dibutyl Phthalate ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Chenopodium Quinoa ◽  
Ethyl Acetate Fraction ◽  
Ms Analysis ◽  
Benzenedicarboxylic Acid

Quinoa (Chenopodium quinoa L.) is a recently introduced pseudocereal crop in Pakistan. In the present study, methanolic extract was partitioned with n-hexane, chloroform and finally with ethyl acetate. The ethyl acetate fraction was subjected to GC-MS analysis for the identification of various antimicrobial and antioxidant constituents. Thirteen compounds were identified through GC-MS analysis of this fraction. Among these, 11 compounds possessed antibacterial, antifungal and/or antioxidant properties as reported in literature. These include naphthalene; tetradecane; hexadecane; pentadecane; cyclohexadecane; 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester; dibutyl phthalate; 1-nonadecene; 1-octadecanol; 9-hexacosene; and 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester. 

scholarly journals Comparative phytotoxicity and chironomid toxicity assessments of leaf successive extract fractions of Asiatic poison bulb, Crinum asiaticum L

2020 ◽  
Author(s):  
Souren Goswami ◽  
Sanjib Ray
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Meristematic Tissue ◽  
Aqueous Fraction ◽  
Extract Fraction ◽  
Potent Growth ◽  
Crinum Asiaticum

AbstractCrinum asiaticum is an evergreen bulbous perennial shrub of Amaryllidaceae family with ethnomedicinal importance and our earlier study described a comparative account antimicrobial and antioxidant properties of the different solvent-mediated sequential extract fractions. The present study aimed to analyze their comparative account of induced phytotoxicity and chironomid toxicity. For phytotoxicity assessment, germination inhibition and seedling’s root and shoot growth retardation effects on Triticum aestivum and Cicer arietinum were analyzed and for lethal concentration determination, the freshwater bottom-dwelling first instars chironomid larvae were used. The crude aqueous, petroleum ether and ethyl acetate extracts showed significant toxic effects on both meristematic tissue and aquatic midges. The phytotoxic assays indicate that the ethyl acetate fraction contains the most potent growth inhibitors, followed by the crude aqueous and petroleum ether fractions. The last aqueous fraction was found to be the least toxic, the highest LC50 and LT50 values and ethyl acetate extract fraction having highest toxicity. Thus the present study supplements to our earlier report, that indicated the last aqueous extract fraction of C. asiaticum has potent antioxidant and antibacterial potentials as well as its prospective use in livestock maintenance, as it is least toxic and the ethyl acetate extract, the most toxic fraction identified here, is needed to explore for pharmaceutical importance.

Antioxidant and Anti-inflammatory Activities of Organic and Aqueous Extracts of Northeast Algerian Marrubium vulgare

2018 ◽  
Vol 16 (S1) ◽  
pp. S119-S129
Author(s):  
I. Namoune ◽  
B. Khettal ◽  
A.M. Assaf ◽  
S. Elhayek ◽  
L. Arrar
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Ethyl Acetate Extract ◽  
Interleukin 1 ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Aqueous Extracts ◽  
Traditional Use ◽  
Marrubium Vulgare ◽  
Anti Inflammatory

Marrubium vulgare (Lamiaceae) is frequently used in traditional medicine to treat many illnesses from ancient times. Its beneficial effects include antibacterial, antioedematogenic, and analgesic activities. This study was designed to evaluate the antioxidant and anti-inflammatory activities of organic and aqueous extracts of the leaves, the flowers, the stems, and the roots of Marrubium vulgare. The total phenolic and flavonoid contents as well as the antioxidant and the anti-inflammatory effects of methanol, chloroform, ethyl acetate, and aqueous extracts have been investigated by using different in-vitro methods. It was found that the ethyl acetate extract from Marrubium vulgare stems had the highest total phenolic content, while the ethyl acetate extract from the leaves yielded a high concentration of flavonoids. The ethyl acetate extract from the stems exhibited the highest activity in scavenging of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), as well as in protecting erythrocytes. The leaves aqueous extract exhibited the highest ferrous chelating activity and its methanolic extract was found to be the strongest inhibitor of lipid peroxidation in β-carotene bleaching assay. The leaves chloroform extracts as well as the flowers methanol, chloroform, and ethyl acetate extracts were found to decrease the pro-inflammatory tumor necrosis factor alpha (TNF-α) cytokine levels in a dose-dependent manner. On the other hand, the flowers methanolic extract and the leaves methanol, ethyl acetate, and aqueous extracts decreased the interleukin-1 beta (IL- 1β) release. It was also found that the methanol extract from the flowers and the chloroform extract from the stems of Marrubium vulgare inhibited interleukin-8 (IL-8) release. This study provides a scientific basis for the traditional use of Marrubium vulgare as an anti-inflammatory agent and for the plant to be considered as an important resource of natural antioxidants.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals Marine Natural Products, IX A New Halogenated Sesquiterpene Pinnatifidone from the Red Alga Laurencia pinnatifida

1988 ◽  
Vol 43 (10) ◽  
pp. 1347-1350 ◽  
Author(s):  
Shaheen Bano ◽  
Mohammad Shaiq Ali ◽  
Viqar Uddin Ahmad
Keyword(s):  
Natural Products ◽  
Ethyl Acetate ◽  
Marine Natural Products ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Spectroscopic Studies ◽  
Red Alga

Abstract From the ethyl acetate fraction of methanolic extract of red alga, L.pinnatifida, a new halogenated sesquiterpene named as pinnatifidone [1] has been isolated and the structure of this compound has been elucidated with the help of intensive spectroscopic studies.

scholarly journals Sustainable Biosynthesis of Antioxidants from Koji Rice Fermented with Aspergillus flavus Using Microwave-Assisted Extraction

2021 ◽  
Vol 11 (1) ◽  
pp. 430
Author(s):  
Hassan Hadi Mehdi Al Rubaiy ◽  
Ammar Altemimi ◽  
Ali Khudair Jaber Al Rikabi ◽  
Naoufal Lakhssassi ◽  
Anubhav Pratap-Singh
Keyword(s):  
Ethyl Acetate ◽  
Aspergillus Flavus ◽  
Bioactive Compounds ◽  
Ethylenediaminetetraacetic Acid ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Antioxidant Compounds ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction

The present study proposes microwave-assisted extraction as a sustainable technique for the biosynthesis of bioactive compounds from rice fermented with Aspergillus flavus (koji). First, fermentation conditions (i.e., pH from 3–12, five temperatures from 20–40 °C, and four culture-fermentation media viz. wheat, wheat bran, malt and rice) were optimized for producing microbial bioactive compounds. Microwave extraction was performed at 2450 MHz and 500 W for 20, 30, and 40 s with seven solvents (distilled water, ethyl acetate, hexane, ethanol, chloroform, diethyl ether, and methanol). The obtained results revealed that ethyl acetate is the most appropriate solvent for extraction. Effects of this ethyl acetate extract were compared with a commercial synthetic antioxidant. Antioxidant properties were enhanced by preventing the oxidation of the linoleic acid (C18H32O2) with an inhibition rate (antioxidant efficacy) of 73.13%. Notably, the ferrous ion binding ability was marginally lower when compared to the disodium salt of ethylenediaminetetraacetic acid (EDTA). Additionally, the obtained total content of phenolic compounds in the ethyl acetate extract of fermented rice (koji) by Aspergillus flavus was 232.11 mg based on gallic acid/mL. Antioxidant compounds in the ethyl acetate extract of fermented rice showed stability under neutral conditions, as well as at high temperatures reaching 185 °C during 2 h, but were unstable under acidic and alkaline conditions. The results demonstrate the efficacy of novel microwave-assisted extraction technique for accelerating antioxidant production during rice fermentation.

scholarly journals The ethyl acetate fraction of a methanolic extract of unripe noni (Morinda citrifolia Linn.) fruit exhibits a biphasic effect on the dopaminergic system in mice

2017 ◽  
Vol 66 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Vijayapandi Pandy ◽  
Megala Narasingam ◽  
Kamini Vijeepallam ◽  
Syam Mohan ◽  
Vasudevan Mani ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Dopaminergic System ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Morinda Citrifolia ◽  
Biphasic Effect

HPLC Analysis and Antioxidant Properties of Algerian Lepidium draba Ethyl acetate Extract

2018 ◽  
Vol 8 (4) ◽  
pp. 265-271
Author(s):  
Bensaid Sara Ouissem ◽  
Bicha Sabrina ◽  
Benmekhebi Lotfi ◽  
Rebbas Khellaf ◽  
Bensouici Chawki ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Hplc Analysis ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Lepidium Draba

scholarly journals Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract

2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Antioxidant Properties ◽  
Linear Connection ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Control Group

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.

scholarly journals Antioxidant and antimicrobial activities against Enterobacter cloacae of extracts of Eclipta alba Hassk.

2016 ◽  
Vol 19 (4) ◽  
pp. 76-83
Author(s):  
Trang Thi Xuan Dai ◽  
Anh Thi Tu Vo
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enterobacter Cloacae ◽  
Free Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Eclipta Alba

This study was subjected to investigate antimicrobial and antioxidant properties of Eclipta alba extracts (methanol, hexane, chloroform and ethyl acetate extracts). The antioxidant property of the extract was assessed by 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Kirby-Bauer method was used to determine the antibacterial activity against Enterobacter cloacae of the extracts. The extracts of Eclipta alba were tested against E. cloacae. The highest antibacterial potentiality was exhibited by the ethyl acetate extract of E. alba leaves, means of zones of bacterial growth inhibition are 26.3 mm at a concentration of 32 μg/mL. DPPH free radical scavenging effect of the extracts was compared with standard antioxidant vitamin C. The highest antioxidant activity was exhibited by the ethyl acetate extract of E. alba leaves. The result also showed that the DPPH scavenging activity of ethyl acetate extract from leaves of E. alba was high (EC50 = 419.38 μg/mL). However, this result was 18.99 times lower than that of vitamin C (EC50 = 22.08 μg/mL).

scholarly journals Uji Efektivitas Bioherbisida Ekstrak Etil Asetat Babadotan (Ageratum conyzoides L.) Dari Berbagai Ketinggian Tempat Dan Konsentrasi Terhadap Pertumbuhan Gulma Bayam Duri (Amaranthus spinosus L.)

2020 ◽  
Vol 3 (4) ◽  
pp. 34-42
Author(s):  
Zainuddin Zainuddin ◽  
Siti Hafsah ◽  
Gina Erida
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
High Rate ◽  
Ageratum Conyzoides ◽  
Amaranthus Spinosus ◽  
Number Of Leaves ◽  
Materials Used ◽  
Banda Aceh ◽  
Ethyl Acetate Extracts

Abstrak. Penelitian ini bertujuan untuk menguji aktivitas ekstrak etil asetat babadotan dari berbagai ketinggian tempat dan konsentrasi terhadap pertumbuhan gulma bayam duri. Penelitian ini dilaksanakan di Laboratorium Ilmu Gulma Program Studi Agroteknologi dan Kebun Percobaan Fakultas Pertanian Universitas Syiah Kuala Banda Aceh, serta Laboratorium Analisis Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA), sejak September 2017– Januari 2018. Bahan yang digunakan adalah benih bayam duri, yang diperoleh dari Kecamatan Delima Kabupaten Pidie dan daun babadotan yang diperoleh dari 3 daerah dengan ketinggian yang berbeda yaitu Kecamatan Indrapuri Kabupaten Aceh Besar (19 mdpl), Kecamatan Lembah Seulawah Saree Kabupaten Aceh Besar (261 mdpl) dan Kecamatan Takengon Kabupaten Aceh Tengah (1259 mdpl) dengan konsentrasi 5, 10 dan 15% sebagai bioherbisida. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola faktorial 3  3 dengan 3 kali ulangan. Faktor pertama yaitu ketinggian tempat dengan 3 taraf dan faktor kedua konsentrasi dengan 3 taraf. Ekstrak yang dihasilkan pada ketinggian tempat yang berbeda dan konsentrasi ekstrak etil asetat berpengaruh nyata terhadap laju tinggi, jumlah daun, diameter batang, persentase pengendalian dan panjang akar. Terdapat interaksi antara ketinggian tempat dan konsentrasi terhadap persentase pengendalian pada 7 dan 14 HSA. Ekstrak yang dihasilkan pada ketinggian  9 mdpl dengan konsentrasi 10% dapat mengendalikan gulma bayam duri hingga 100% pada 7 HSA.The Test of Effectiveness Whiteweed Ethyl Acetate Extract (Ageratum conyzoides L.) from Various Altitudes And Concentration Against the Growth of Spiny Amaranth Weed (Amaranthus spinosus L.)Abstract. This study aims to examine the activity of ethyl acetate fraction of whiteweed extract from various altitudes and concentration againts the growth of spiny amaranth. This  research was conducted at Laboratory of Weed Science, Agrotechnology Department, and Experimental Garden, Agriculture Faculty, Syiah Kuala University Banda Aceh, and Laboratory of Chemical Analysis Mathematics Faculty and Natural Science, from September 2017 to January 2018. The materials used is spiny amaranth seed that obtained from Subdistrict of Delima, District of Pidie and babadotan leaves obtained from three regions with different altitude that is Subdistrict of Indrapuri, District of Aceh Besar (19 masl), Subdistrict of Subdistrict of, District of Aceh Besar (261 masl) and Subdistrict of Takengon District of Aceh Tengah (1259 masl) with each concentration 5, 10 and 15% as bioherbicide. This research using Randomized Completely Design with Factorial pattern which has 3 × 3 treatments and 3 replication. The first faktor is altitude and second is concentration of ethyl acetate extract. Ethyl acetate extracts that produced in different altitudes and concentrations were significantly influence the high rate of spiny amaranth, number of leaves, stem diameter, control percentage and root length. There is an interaction between altitude and concentration on the percentage of control at 7 and 14 HSA. Extracts produced at an altitude of 9 masl with a concentration of 10% can control spinach weeds up to 100% at 7 HSA.

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