scholarly journals Genetic Control Diversity Drives Differences Between Cadmium Distribution and Tolerance in Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Yi-Bo Chen ◽  
Yu-Chao Chen ◽  
Yu-Xing Zhu ◽  
Sai Li ◽  
Hua-bing Deng ◽  
...  
Keyword(s):  
Genetic Control ◽  
Genotypic Diversity ◽  
Rice Varieties ◽  
Cd Tolerance ◽  
Genetic Patterns ◽  
Genetic Mechanisms ◽  
Low Levels ◽  
Cd Distribution ◽  
Staple Crop

Rice, a staple crop for nearly half the planet’s population, tends to absorb and accumulate excessive cadmium (Cd) when grown in Cd-contaminated fields. Low levels of Cd can degrade the quality of rice grains, while high levels can inhibit the growth of rice plants. There is genotypic diversity in Cd distribution and Cd tolerance in different rice varieties, but their underlying genetic mechanisms are far from elucidated, which hinders genetic improvements. In this study, a joint study of phenotypic investigation with quantitative trait loci (QTLs) analyses of genetic patterns of Cd distribution and Cd tolerance was performed using a biparent population derived from japonica and indica rice varieties. We identified multiple QTLs for each trait and revealed that additive effects from various loci drive the inheritance of Cd distribution, while epistatic effects between various loci contribute to differences in Cd tolerance. One pleiotropic locus, qCddis8, was found to affect the Cd distribution from both roots to shoots and from leaf sheaths to leaf blades. The results expand our understanding of the diversity of genetic control over Cd distribution and Cd tolerance in rice. The findings provide information on potential QTLs for genetic improvement of Cd distribution in rice varieties.

scholarly journals The Relationship between Cadmium Toxicity and the Modulation of Epigenetic Traits in Plants

2021 ◽  
Vol 22 (13) ◽  
pp. 7046
Author(s):  
Lee-Ann Niekerk ◽  
Mogamat Fahiem Carelse ◽  
Olalekan Olanrewaju Bakare ◽  
Vuyo Mavumengwana ◽  
Marshall Keyster ◽  
...  
Keyword(s):  
Stress Responses ◽  
Crop Production ◽  
Negative Impact ◽  
Cadmium Toxicity ◽  
Cd Stress ◽  
Cd Tolerance ◽  
Genetic Mechanisms ◽  
Staple Crop ◽  
Future Work ◽  
The Relationship

Elevated concentrations of heavy metals such as cadmium (Cd) have a negative impact on staple crop production due to their ability to elicit cytotoxic and genotoxic effects on plants. In order to understand the relationship between Cd stress and plants in an effort to improve Cd tolerance, studies have identified genetic mechanisms which could be important for conferring stress tolerance. In recent years epigenetic studies have garnered much attention and hold great potential in both improving the understanding of Cd stress in plants as well as revealing candidate mechanisms for future work. This review describes some of the main epigenetic mechanisms involved in Cd stress responses. We summarize recent literature and data pertaining to chromatin remodeling, DNA methylation, histone acetylation and miRNAs in order to understand the role these epigenetic traits play in cadmium tolerance. The review aims to provide the framework for future studies where these epigenetic traits may be used in plant breeding and molecular studies in order to improve Cd tolerance.

scholarly journals Effects of introduction of combine harvester and flatbed dryer on milling quality of three glutinous rice varieties in Lao PDR

2018 ◽  
Vol 22 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Khamtay Vongxayya ◽  
Darunee Jothityangkoon ◽  
Danuphol Ketthaisong ◽  
Jaquie Mitchell ◽  
Phetmanyseng Xangsayyasane ◽  
...  
Keyword(s):  
Lao Pdr ◽  
Rice Varieties ◽  
Milling Quality ◽  
Glutinous Rice ◽  
Combine Harvester

scholarly journals Comparison of Morphological and Physicochemical Properties of a Floury Rice Variety upon Pre-Harvest Sprouting

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 746
Author(s):  
Chae-Min Han ◽  
Jong-Hee Shin ◽  
Jung-Bae Kwon ◽  
Jong-Soo Kim ◽  
Jong-Gun Won ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Rice Variety ◽  
Wheat Variety ◽  
Economic Loss ◽  
Rice Grain ◽  
Starch Granules ◽  
Rice Varieties ◽  
Starch Structure ◽  
Gelatinization Properties

Pre-harvest sprouting (PHS) severely reduces rice grain yield, significantly affects grain quality, and leads to substantial economic loss. In this study, we aimed to characterize the physicochemical properties and processing quality of the Garumi 2 flour rice variety under PHS conditions and compare them with those of the Seolgaeng, Hangaru, Shingil, and Ilpum rice varieties and the Keumkang wheat variety. Analysis of the molecular structure of starch revealed uniform starch granules, increased proportions of short-chain amylopectin in DP 6–12 (51.0–55.3%), and enhanced crystallinity (30.7–35.7%) in rice varieties for flour compared with the Ilpum cooking rice variety. PHS significantly altered the starch structure and gelatinization properties of Garumi 2. It also caused surface pitting and roughness in Garumi 2 starch granules and decreased their crystallinity. Collectively, the findings of this study provide important novel insights into the effects of PHS on the physicochemical properties of Garumi 2 floury rice for flour.

scholarly journals Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 806
Author(s):  
Moez Maghrebi ◽  
Elena Baldoni ◽  
Giorgio Lucchini ◽  
Gianpiero Vigani ◽  
Giampiero Valè ◽  
...  
Keyword(s):  
Transcript Abundance ◽  
Root Cells ◽  
Expression Levels ◽  
Cd Tolerance ◽  
Relative Contribution ◽  
Relative Transcript Abundance ◽  
Potential Capacity ◽  
Cd Distribution ◽  
Cd Exposure ◽  
Cd Availability

Two rice accessions, Capataz and Beirao, contrasting for cadmium (Cd) tolerance and root retention, were exposed to a broad range of Cd concentrations (0.01, 0.1, and 1 μM) and analyzed for their potential capacity to chelate, compartmentalize, and translocate Cd to gain information about the relative contribution of these processes in determining the different pathways of Cd distribution along the plants. In Capataz, Cd root retention increased with the external Cd concentration, while in Beirao it resulted independent of Cd availability and significantly higher than in Capataz at the lowest Cd concentrations analyzed. Analysis of thiol accumulation in the roots revealed that the different amounts of these compounds in Capataz and Beirao, as well as the expression levels of genes involved in phytochelatin biosynthesis and direct Cd sequestration into the vacuoles of the root cells, were not related to the capacity of the accessions to trap the metal into the roots. Interestingly, the relative transcript abundance of OsHMA2, a gene controlling root-to-shoot Cd/Zn translocation, was not influenced by Cd exposure in Capataz and progressively increased in Beirao with the external Cd concentration, suggesting that activity of the OsHMA2 transporter may differentially limit root-to-shoot Cd/Zn translocation in Capataz and Beirao.

scholarly journals AB0050 A NOVEL METHOD FOR ISOLATION OF EXOSOMES FROM SYNOVIAL FLUID

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1057.2-1057
Author(s):  
Y. Liu ◽  
Y. Huang ◽  
Q. Huang ◽  
S. Sun ◽  
Z. Ji ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Western Blot ◽  
Biological Fluid ◽  
High Sensitivity ◽  
Protein Distribution ◽  
Sds Page ◽  
Close Relationship ◽  
Novel Method ◽  
Low Levels

Background:Exosomes in synovial fluid (SF) has a close relationship with the pathogenesis of rheumatiod arthritis. As a complex biological fluid, SF presents challenges for exosomes isolation using standard methods, such as ExoquickTM kit and ultracentrifugation.Objectives:The study aims to compared the quality of exosomes separated by ExoquickTM kit (TM), ExoquickTM kit+ExoquickTC kit (TM-TC), ultracentrifugation (UC) and TM-TC+UC(TM-TC-UC) from SF.Methods:Exosomes was separated by TM, TM-TC, UC and TM-TC-UC respectively. The size and concentrations of exosomes were detected by high sensitivity flow cytometry for nanoparticle analysis. Total protein and RNA were extracted from exosomes. SDS-PAGE was used to detect the protein distribution of exosomes. Western blot was used to examine the level of albumin and exosomes marker (TSG101 and CD81).Results:There was no statistic difference in the diameters of exosomes separated by the four methods. The concentrations of exosomes in TM, TM-TC, TM-TC-UC and UC were (5.65±0.93), (3.02±1.19), (1.67±0.25) and (4.61±0.73) *109Particles/mL. The protein concentrations of exosomes separated by the four methods were consistent with the concentrations of exosomes. SDS-PAGE showed that the protein distribution of exosomes separated by the four methods were different. Low levels of albumin were detected in TM-TC and TM-TC-UC, while high levels of albumin in TM and UC. Total RNA concentrations from exosomes in TM-TC was higher than other groups.Conclusion:TM-TC can be used to obtain higher quality exosomes from SF for the study of exosome-enriched components.References:[1]Helwa I, et al, A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents. PloS one, 2017. 12(1): p. e0170628-e0170628.Figure 1.A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot.Disclosure of Interests:None declared

scholarly journals The emergence of coercive federal Australian labour law, 1901–2020

2021 ◽  
pp. 002218562110039
Author(s):  
Eugene Schofield-Georgeson
Keyword(s):  
Industrial Relations ◽  
High Volume ◽  
Union Power ◽  
Labour Law ◽  
Industrial Environment ◽  
The Past ◽  
Low Levels ◽  
Labour Legislation ◽  
Employment Legislation

Over the past two decades, industrial relations scholarship has observed a trend towards an increasingly punitive industrial environment along with the ‘re-regulation’ of labour law. Absent from much of this literature, however, has been an empirical and historical measurement or comparison of the scale and quality of this systemic change. By surveying coercive and penal federal industrial legislation over the period 1901–2020, this study shows empirically that over the last 40 years, there has been a steep increase in the amount of coercive federal labour legislation in Australia. It further measures and compares the volume of coercive labour legislation enacted specifically against ‘labour’ and ‘capital’ or both throughout the same period (1901–2020). Analysis reveals a correlation between a high volume of coercive labour legislation with low levels of trade union power and organisation. Argued here is that coercive labour legislation has been crucial to transitioning from a liberal conciliation and arbitration model of Australian industrial relations towards a neoliberal framework of employment legislation. In the former, regulation was more collective, informal and egalitarian (embodied by the sociological concept of ‘associative democracy’). Under a neoliberal framework, regulation is now more individualised, technical, punitive and rarely enforced, resulting in less equal material outcomes.

scholarly journals The effect of amino-acid-supplemented wheat gluten on cholesterol metabolism in the rat

1985 ◽  
Vol 53 (1) ◽  
pp. 25-30 ◽  
Author(s):  
M. Bassat ◽  
S. Mokady
Keyword(s):  
Amino Acid ◽  
Cholesterol Metabolism ◽  
Cholesterol Biosynthesis ◽  
Wheat Gluten ◽  
Blood Cholesterol ◽  
Amino Acid Supplementation ◽  
Faecal Excretion ◽  
Weanling Rats ◽  
Low Levels

1. The effect of lysine- and threonine-supplemented wheat gluten on cholesterol metabolism was studied using male weanling rats. Animals were fed on cholesterol-free diets containing 100 or 200 g gluten/kg with or without amino acid supplementation, and compared with animals given 50, 100 and 200 g casein/kg diets, for 3 weeks.2. A hypocholesterolaemic effect observed with the wheat gluten-fed rats, compared with the animals given 100 and 200 g casein/kg diets, was accompanied by increased turnover of cholesterol as expressed by enhanced cholesterol biosynthesis and increased faecal excretion of cholesterol and bile acids. This effect was not abolished by lysine and threonine supplementation.3. Low levels of blood cholesterol were also observed in the rats fed on the 50 g casein/kg diet. However, a different mechanism, related to impairment of cholesterol transport from the liver, was most likely responsible for the hypocholesterolaemia found in these protein-malnourished animals.4. The effect on cholesterol metabolism produced by dietary wheat gluten was independent of the low quality of the protein and of its specific deficiency in lysine and threonine.

CHARACTERISTICS OF MILLING YIELD OF RICE VARIETIES OF RUSSIAN BREEDING GROWN IN VARIOUS CONDITIONS OF NITROGEN NUTRITION IN THE ABINSKIY DISTRICT OF THE KRASNODAR REGION

2020 ◽  
Author(s):  
E.Y. Papulova ◽  
◽  
K.K Olkhovaya
Keyword(s):  
Grain Quality ◽  
Rice Yield ◽  
Nitrogen Nutrition ◽  
Nitrogen Fertilizers ◽  
Rice Varieties ◽  
Head Rice ◽  
Cultivation Technology ◽  
Milling Yield ◽  
Different Doses

The work shows that application of increased doses of nitrogen does not significantly affect the total milling yield and affects the head rice content. The ambiguous nature of the variability of the grain quality of rice varieties under conditions of different doses of nitrogen fertilizers confirms the need for further studies of the varietal reaction to the level of nitrogen nutrition, based on it - the development of rice cultivation technology modes in order to obtain rice yield with high grain quality.

Microbiological Quality of Commercial Tofu

1984 ◽  
Vol 47 (3) ◽  
pp. 177-181 ◽  
Author(s):  
T. G. REHBERGER ◽  
L. A. WILSON ◽  
B. A. GLATZ
Keyword(s):  
Microbiological Quality ◽  
Plate Count ◽  
Microbial Counts ◽  
Colony Forming Units ◽  
Low Levels ◽  
Microbial Groups ◽  
Yeasts And Molds ◽  
Processing Techniques

A study was done to investigate the microbiological quality of commercial tofu available in local retail outlets. A sampling method was first developed to obtain accurate and representative microbial counts of individual pieces of tofu. Plate count determination of total aerobic organisms, psychrotrophs, coliforms, sporeformers, yeasts and molds, and staphylococci were made on 60 tofu samples (representing three lots each of four different brands) obtained within 24 h after delivery to the retail store. In addition, for two brands that provided manufacturer's pull dates, the same microbial counts were obtained for samples stored in the laboratory at 10°C until the pull date. Of the tofu sampled immediately after purchase, 83% of the lots tested had total counts greater than 106 colony-forming units (CFU)/g and psychrotrophic counts greater than 104 CFU/g. In addition, 67% of the lots tested had confirmed coliform counts greater than 103 CFU/g. Very low levels (less than 10 CFU/g) of all other microbial groups tested for were found in the majority of lots. Samples held until the manufacturer's pull date contained higher total and psychrotrophic counts but lower or stable counts of other organisms compared with samples tested immediately after purchase. To improve the microbiological quality of tofu, processors need to reduce initial loads by improving sanitation and processing techniques, and retailers should provide more consistent and colder refrigerated storage.

Education and Anti-Immigration Attitudes: Evidence from Compulsory Schooling Reforms across Western Europe

2018 ◽  
Vol 113 (1) ◽  
pp. 254-263 ◽  
Author(s):  
CHARLOTTE CAVAILLE ◽  
JOHN MARSHALL
Keyword(s):  
Secondary School ◽  
Western Europe ◽  
Future Research ◽  
Compulsory Schooling ◽  
Far Right ◽  
Secondary Schooling ◽  
Low Levels ◽  
Immigration Attitudes ◽  
Post War

Low levels of education are a powerful predictor of anti-immigration sentiment. However, there is little consensus on the interpretation of this correlation: is it causal or is it an artifact of selection bias? We address this question by exploiting six major compulsory schooling reforms in five Western European countries—Denmark, France, Great Britain, the Netherlands, and Sweden—that have recently experienced politically influential anti-immigration movements. On average, we find that compelling students to remain in secondary school for at least an additional year decreases anti-immigration attitudes later in life. Instrumental variable estimates demonstrate that, among such compliers, an additional year of secondary schooling substantially reduces the probability of opposing immigration, believing that immigration erodes a country’s quality of life, and feeling close to far-right anti-immigration parties. These results suggest that rising post-war educational attainment has mitigated the rise of anti-immigration movements. We discuss the mechanisms and implications for future research examining anti-immigration sentiment.

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