scholarly journals ThCOL2 Improves the Salt Stress Tolerance of Tamarix hispida

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaojin Lei ◽  
Bing Tan ◽  
Zhongyuan Liu ◽  
Jing Wu ◽  
Jiaxin Lv ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Cell Damage ◽  
Expression Patterns ◽  
Nacl Stress ◽  
Tamarix Hispida ◽  
Resistance Response ◽  
Expression Levels ◽  
Qrt Pcr

The CONSTANS-LIKE (COL) transcription factor has been reported to play important roles in regulating plant flowering and the response to abiotic stress. To clone and screen COL genes with excellent salt tolerance from the woody halophyte Tamarix hispida, 8 ThCOL genes were identified in this study. The expression patterns of these genes under different abiotic stresses (high salt, osmotic, and heavy metal) and abscisic acid (ABA) treatment were detected using quantitative real-time PCR (qRT-PCR). The expression levels of 8 ThCOL genes changed significantly after exposure to one or more stresses, indicating that these genes were all stress-responsive genes and may be involved in the stress resistance response of T. hispida. In particular, the expression level of ThCOL2 changed significantly at most time points in the roots and leaves of T. hispida under salt stress and after ABA treatments, which may play an important role in the response process of salt stress through a mechanism dependent on the ABA pathway. The recombinant vectors pROKII–ThCOL2 and pFGC5941–ThCOL2 were constructed for the transient transformation of T. hispida, and the transient infection of T. hispida with the pROKII empty vector was used as the control to further verify whether the ThCOL2 gene was involved in the regulation of the salt tolerance response of T. hispida. Overexpression of the ThCOL2 gene in plants under 150 mM NaCl stress increased the ability of transgenic T. hispida cells to remove reactive oxygen species (ROS) by regulating the activity of protective enzymes and promoting a decrease in the accumulation of O2– and H2O2, thereby reducing cell damage or cell death and enhancing salt tolerance. The ThCOL2 gene may be a candidate gene associated with excellent salt tolerance. Furthermore, the expression levels of some genes related to the ABA pathway were analyzed using qRT-PCR. The results showed that the expressions of ThNCED1 and ThNCED4 were significantly higher, and the expressions of ThNCED3, ThZEP, and ThAAO3 were not significantly altered in OE compared with CON under normal conditions. But after 24 h of salt stress, the expressions of all five studied genes all were lower than the normal condition. In the future, the downstream genes directly regulated by the ThCOL2 transcription factor will be searched and identified to analyze the salt tolerance regulatory network of ThCOL2.

scholarly journals Overexpression of Tamarix hispida ThTrx5 Confers Salt Tolerance to Arabidopsis by Activating Stress Response Signals

2020 ◽  
Vol 21 (3) ◽  
pp. 1165
Author(s):  
Jiayu Luan ◽  
Jingxiang Dong ◽  
Xin Song ◽  
Jing Jiang ◽  
Huiyu Li
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Germination Rate ◽  
Nacl Stress ◽  
Tamarix Hispida ◽  
Resistant Varieties ◽  
Transcriptome Comparison ◽  
Plant Salt Tolerance ◽  
Cellular Water

Salt stress inhibits normal plant growth and development by disrupting cellular water absorption and metabolism. Therefore, understanding plant salt tolerance mechanisms should provide a theoretical basis for developing salt-resistant varieties. Here, we cloned ThTrx5 from Tamarix hispida, a salt-resistant woody shrub, and generated ThTrx5-overexpressing transgenic Arabidopsis thaliana lines. Under NaCl stress, the germination rate of overexpressing ThTrx5 lines was significantly increased relative to that of the nontransgenic line; under salt stress, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione levels and root length and fresh weight values of transgenic ThTrx5 plants were significantly greater than corresponding values for wild-type plants. Moreover, with regard to the transcriptome, comparison of differential gene expression of transgenic versus nontransgenic lines at 0 h and 3 h of salt stress exposure revealed 500 and 194 differentially expressed genes (DEGs), respectively, that were mainly functionally linked to catalytic activity and binding process. Pull-down experiments showed that ThTrx bound 2-Cys peroxiredoxin BAS1-like protein that influences stress response-associated redox, hormone signal transduction, and transcription factor functions. Therefore, this work provides important insights into ThTrx5 mechanisms that promote salt tolerance in plants.

scholarly journals Identification and Evolutionary Characteristic Analysis of STARD Gene Family, and Overexpression VvSTARD5 Responses to Salt Stress in Tomato

2022 ◽  
Author(s):  
Honghong He ◽  
Shixiong lu ◽  
Huiming Gou ◽  
Xuejing Cao ◽  
Ping Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Gene Family ◽  
Tertiary Structure ◽  
Function Analysis ◽  
Expression Profiles ◽  
Regulatory Gene ◽  
Characteristic Analysis ◽  
Expression Levels ◽  
Qrt Pcr

Abstract This study aimed to have a full understanding of the steroidogenic acute regulatory gene family member and evolutionary relationship in grape. 23 VvSTARD gene members were identified and divided into five groups in different species. Analyses of the gene codon preference, selective pressure, and tandem duplication of the VvSTARD, AtSTARD, and OsSTARD genes indicated that synteny relationship occurred in grapes, Arabidopsis thaliana, and rice genomes. The 8 lipid transporter proteins were found in the tertiary structure of the STARD gene family in grape. Expression profiles of the three species microarrays showed that the expression levels of the STARD genes in different organs and the response to abiotic stress in the same subgroup had similar characteristics. In addition, analysis of the VvSTARD genes expression levels was detected in response to different hormones and abiotic stresses by quantitative real-time polymerase chain reaction (qRT-PCR), and the results were the same as those predicted by the cis-elements and the expression profiles. Meanwhile, VvSTARD5 gene was screened in high concentration NaCl treatment by qRT-PCR. Furthermore, the VvSTARD5 was located at the nucleus by subcellular location. Through the function analysis of salt tolerance in transgenic tomato, overexpression VvSTARD5 obviously improved tolerance to salt stress. Taken together, our findings Preliminary identify the functions of VvSTARD gene family and vertify STARD5 that be likely involved in regulating salt tolerance, which may have potential application molecular breeding in grape.

scholarly journals Rice Cultivars Under Salt Stress Show Differential Expression of Genes Related to the Regulation of Na+/K+ Balance

2021 ◽  
Vol 12 ◽  
Author(s):  
Muhammad Farooq ◽  
Jae-Ryoung Park ◽  
Yoon-Hee Jang ◽  
Eun-Gyeong Kim ◽  
Kyung-Min Kim
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Germination Rate ◽  
Expression Patterns ◽  
Germination Percentage ◽  
Yield Reduction ◽  
High Accumulation ◽  
Expression Levels ◽  
Ion Concentrations ◽  
Expression Of Genes

Soil salinity is a major problem in agriculture because high accumulation of Na+ ions in plants causes toxicity that can result in yield reduction. Na+/K+ homeostasis is known to be important for salt tolerance in plants. Na+/K+ homeostasis in rice (Oryza sativa L.) involves nine high-affinity K+ transporter (HKT) encoding Na+-K+ symporter, five OsNHX Na+/H+ antiporters, and OsSOS1 Na+/K+ antiporter genes. In the present study, we investigated various molecular and physiological processes to evaluate germination rate, growth pattern, ion content, and expression of OsHKT, OsNHX, and OsSOS1genes related to Na+/K+ homeostasis in different rice genotypes under salt stress. We found a significant increase in the germination percentage, plant vigor, Na+/K+ ratio, and gene expression of the OsHKT family in both the roots and shoots of the Nagdong cultivar and salt-tolerant cultivar Pokkali. In the roots of Cheongcheong and IR28 cultivars, Na+ ion concentrations were found to be higher than K+ ion concentrations. Similarly, high expression levels of OsHKT1, OsHKT3, and OsHKT6 were observed in Cheongcheong, whereas expression levels of OsHKT9 was high in IR28. The expression patterns of OsNHX and OsSOS1 and regulation of other micronutrients differed in the roots and shoots regions of rice and were generally increased by salt stress. The OsNHX family was also expressed at high levels in the roots of Nagdong and in the roots and shoots of Pokkali; in contrast, comparatively low expression levels were observed in the roots and shoots of Cheongcheong and IR28 (with the exception of high OsNHX1 expression in the roots of IR28). Furthermore, the OsSOS1 gene was highly expressed in the roots of Nagdong and shoots of Cheongcheong. We also observed that salt stress decreases chlorophyll content in IR28 and Pokkali but not in Cheongcheong and Nagdong. This study suggests that under salt stress, cultivar Nagdong has more salt-tolerance than cultivar Cheongcheong.

scholarly journals Characterization of Interactions between the Soybean Salt-Stress Responsive Membrane-Intrinsic Proteins GmPIP1 and GmPIP2

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1312
Author(s):  
Jia Liu ◽  
Weicong Qi ◽  
Haiying Lu ◽  
Hongbo Shao ◽  
Dayong Zhang
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Expression Profiles ◽  
Early Gene ◽  
Plant Responses ◽  
Constitutive Overexpression ◽  
Important Trait

Salt tolerance is an important trait in soybean cultivation and breeding. Plant responses to salt stress include physiological and biochemical changes that affect the movement of water across the plasma membrane. Plasma membrane intrinsic proteins (PIPs) localize to the plasma membrane and regulate the water and solutes flow. In this study, quantitative real-time PCR and yeast two-hybridization were engaged to analyze the early gene expression profiles and interactions of a set of soybean PIPs (GmPIPs) in response to salt stress. A total of 20 GmPIPs-encoding genes had varied expression profiles after salt stress. Among them, 13 genes exhibited a downregulated expression pattern, including GmPIP1;6, the constitutive overexpression of which could improve soybean salt tolerance, and its close homologs GmPIP1;7 and 1;5. Three genes showed upregulated patterns, including the GmPIP1;6 close homolog GmPIP1;4, when four genes with earlier increased and then decreased expression patterns. GmPIP1;5 and GmPIP1;6 could both physically interact strongly with GmPIP2;2, GmPIP2;4, GmPIP2;6, GmPIP2;8, GmPIP2;9, GmPIP2;11, and GmPIP2;13. Definite interactions between GmPIP1;6 and GmPIP1;7 were detected and GmPIP2;9 performed homo-interaction. The interactions of GmPIP1;5 with GmPIP2;11 and 2;13, GmPIP1;6 with GmPIP2;9, 2;11 and GmPIP2;13, and GmPIP2;9 with itself were strengthened upon salt stress rather than osmotic stress. Taken together, we inferred that GmPIP1 type and GmPIP2 type could associate with each other to synergistically function in the plant cell; a salt-stress environment could promote part of their interactions. This result provided new clues to further understand the soybean PIP–isoform interactions, which lead to potentially functional homo- and heterotetramers for salt tolerance.

scholarly journals Transcriptomic Analysis of Betula halophila in Response to Salt Stress

2018 ◽  
Vol 19 (11) ◽  
pp. 3412 ◽  
Author(s):  
Fenjuan Shao ◽  
Lisha Zhang ◽  
Iain Wilson ◽  
Deyou Qiu
Keyword(s):  
Cell Wall ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Expression Patterns ◽  
Soil Salinization ◽  
Sphingolipid Metabolism ◽  
Sequencing Platform ◽  
Resistant Varieties ◽  
High Salt Tolerance ◽  
Salt Overly Sensitive

Soil salinization is a matter of concern worldwide. It can eventually lead to the desertification of land and severely damage local agricultural production and the ecological environment. Betula halophila is a tree with high salt tolerance, so it is of importance to understand and discover the salt responsive genes of B. halophila for breeding salinity resistant varieties of trees. However, there is no report on the transcriptome in response to salt stress in B. halophila. Using Illumina sequencing platform, approximately 460 M raw reads were generated and assembled into 117,091 unigenes. Among these unigenes, 64,551 unigenes (55.12%) were annotated with gene descriptions, while the other 44.88% were unknown. 168 up-regulated genes and 351 down-regulated genes were identified, respectively. These Differentially Expressed Genes (DEGs) involved in multiple pathways including the Salt Overly Sensitive (SOS) pathway, ion transport and uptake, antioxidant enzyme, ABA signal pathway and so on. The gene ontology (GO) enrichments suggested that the DEGs were mainly involved in a plant-type cell wall organization biological process, cell wall cellular component, and structural constituent of cell wall molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment showed that the top-four enriched pathways were ‘Fatty acid elongation’, ‘Ribosome’, ‘Sphingolipid metabolism’ and ‘Flavonoid biosynthesis’. The expression patterns of sixteen DEGs were analyzed by qRT-PCR to verify the RNA-seq data. Among them, the transcription factor AT-Hook Motif Nuclear Localized gene and dehydrins might play an important role in response to salt stress in B. halophila. Our results provide an important gene resource to breed salt tolerant plants and useful information for further elucidation of the molecular mechanism of salt tolerance in B. halophila.

scholarly journals Physiological and Biochemical Responses to Salt Stress of Alfalfa Populations Selected for Salinity Tolerance and Grown in Symbiosis with Salt-Tolerant Rhizobium

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 569
Author(s):  
Annick Bertrand ◽  
Craig Gatzke ◽  
Marie Bipfubusa ◽  
Vicky Lévesque ◽  
Francois P. Chalifour ◽  
...  
Keyword(s):  
Amino Acids ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Water Content ◽  
Salinity Tolerance ◽  
Osmotic Adjustment ◽  
Recurrent Selection ◽  
Aromatic Amino Acids ◽  
Nacl Stress ◽  
Salt Tolerant

Alfalfa and its rhizobial symbiont are sensitive to salinity. We compared the physiological responses of alfalfa populations inoculated with a salt-tolerant rhizobium strain, exposed to five NaCl concentrations (0, 20, 40, 80, or 160 mM NaCl). Two initial cultivars, Halo (H-TS0) and Bridgeview (B-TS0), and two populations obtained after three cycles of recurrent selection for salt tolerance (H-TS3 and B-TS3) were compared. Biomass, relative water content, carbohydrates, and amino acids concentrations in leaves and nodules were measured. The higher yield of TS3-populations than initial cultivars under salt stress showed the effectiveness of our selection method to improve salinity tolerance. Higher relative root water content in TS3 populations suggests that root osmotic adjustment is one of the mechanisms of salt tolerance. Higher concentrations of sucrose, pinitol, and amino acid in leaves and nodules under salt stress contributed to the osmotic adjustment in alfalfa. Cultivars differed in their response to recurrent selection: under a 160 mM NaCl-stress, aromatic amino acids and branched-chain amino acids (BCAAs) increased in nodules of B-ST3 as compared with B-TS0, while these accumulations were not observed in H-TS3. BCAAs are known to control bacteroid development and their accumulation under severe stress could have contributed to the high nodulation of B-TS3.

Physiological characterisation and fine mapping of a salt-tolerant mutant in rice (Oryza sativa)

2015 ◽  
Vol 42 (11) ◽  
pp. 1026 ◽  
Author(s):  
Ping Deng ◽  
Dan Jiang ◽  
Yanmin Dong ◽  
Xingyu Shi ◽  
Wen Jing ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Oryza Sativa L ◽  
Recessive Gene ◽  
Nacl Stress ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
Candidate Locus ◽  
F2 Population

Salt-tolerant mutants are valuable resources for basic and applied research on plant salt tolerance. Here, we report the isolation and characterisation of a salt-tolerant rice (Oryza sativa L.) mutant. This mutant was identified from an ethyl methanesulfonate-induced Nipponbare mutant library, designated as rice salt tolerant 1 (rst1). The rst1 mutant was tolerant to salt stress and showed significantly higher shoot biomass and chlorophyll content, but lower lipid peroxidation and electrolyte leakage under NaCl stress. The improved salt tolerance of this mutant may be due mainly to its enhanced ability to restrict Na+ accumulation in shoots under salt stress conditions. Genetic analysis indicated that the salt tolerance of the rst1 mutant was controlled by a single recessive gene. Quantitative trait locus (QTL) mapping for salt tolerance was performed using an F2 population of rst1 × Peiai 64. Two QTLs were detected, in which the locus on chromosome 6 was determined to be the candidate locus of the rst1 gene. The rst1 locus was subsequently shown to reside within a 270.4-kb region defined by the markers IM29432 and IM29702. This result will be useful for map-based cloning of the rst1 gene and for marker-assisted breeding for salt tolerance in rice.

scholarly journals Transcriptional responses of Rosa rugosa to salt stress and salt shock

2020 ◽  
Vol 44 ◽  
Author(s):  
Michele Valquíria dos Reis ◽  
Laura Vaughn Rouhana ◽  
Patrícia Duarte de Oliveira Paiva ◽  
Diogo Pedrosa Correia da Silva ◽  
Renato Paiva ◽  
...  
Keyword(s):  
Salt Stress ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Exposure Period ◽  
Transcriptional Responses ◽  
Expression Levels ◽  
Salt Shock ◽  
Stress Changes ◽  
Stress Related Genes ◽  
High Level

ABSTRACT Rugosa rugosa has high tolerance to various stresses; however, the molecular mechanisms of this behavior under adverse conditions are unclear. The objective of this study is to investigate expression patterns of stress-related genes in response to salinity stress. Changes in transcript levels of R. rugose, grown under different salt stress conditions (0, 25, 50, and 100 mM NaCl) over a long exposure period (30 days), have been investigated. In addition, the effects of salt shock stress on seedlings exposed to a high level (200 mM) of NaCl for a relatively short duration (3 h) have also been investigated. Expression levels of selected differentially expressed genes have been determined using relative reverse transcription polymerase chain reaction (RT-PCR). It has been observed that seedlings exposed to salt stress for a long duration exhibited no signs of stress in both leaves and roots. In addition, expression of NHX1 in R. rugosa increased in the presence of NaCl. Furthermore, transcripts of EXP4, GPP, NHX1, NAC, and DREB genes also increased under high levels of NaCl. In contrast, expression levels of MYB and TIR decreased during this salt shock treatment. Of particular interest is the increase in levels of transcripts of NHX1 in leaves of seedlings grown under both salt stress and salt shock conditions, thus suggesting that this gene plays an important role in salt stress tolerance in R. rugosa. These findings will support efforts in enhancing salt tolerance in roses, and perhaps in other members of the Rosaceae family.

scholarly journals Agronomical, Physiological and Biochemical Characterization of In Vitro Selected Eggplant Somaclonal Variants under NaCl Stress

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2544
Author(s):  
Sami Hannachi ◽  
Stefaan Werbrouck ◽  
Insaf Bahrini ◽  
Abdelmuhsin Abdelgadir ◽  
Hira Affan Siddiqui
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Normal Growth ◽  
Nacl Stress ◽  
Soluble Carbohydrates ◽  
Regenerated Plants ◽  
And Control ◽  
Physiological And Biochemical

Previously, an efficient regeneration protocol was established and applied to regenerate plants from calli lines that could grow on eggplant leaf explants after a stepwise in vitro selection for tolerance to salt stress. Plants were regenerated from calli lines that could tolerate up to 120 mM NaCl. For further in vitro and in vivo evaluation, four plants with a higher number of leaves and longer roots were selected from the 32 plants tested in vitro. The aim of this study was to confirm the stability of salt tolerance in the progeny of these four mutants (‘R18’, ‘R19’, ‘R23’ and ‘R30’). After three years of in vivo culture, we evaluated the impact of NaCl stress on agronomic, physiological and biochemical parameters compared to the parental control (‘P’). The regenerated and control plants were assessed under in vitro and in vivo conditions and were subjected to 0, 40, 80 and 160 mM of NaCl. Our results show significant variation in salinity tolerance among regenerated and control plants, indicating the superiority of four regenerants (‘R18’, ‘R19’, ‘R23’ and ‘R30’) when compared to the parental line (‘P’). In vitro germination kinetics and young seedling growth divided the lines into a sensitive and a tolerant group. ‘P’ tolerate only moderate salt stress, up to 40 mM NaCl, while the tolerance level of ‘R18’, ‘R19’, ‘R23’ and ‘R30’ was up to 80 mM NaCl. The quantum yield of PSII (ΦPSII) declined significantly in ‘P’ under salt stress. The photochemical quenching was reduced while nonphotochemical quenching rose in ‘P’ under salt stress. Interestingly, the regenerants (‘R18’, ‘R19’, ‘R23’ and ‘R30’) exhibited high apparent salt tolerance by maintaining quite stable Chl fluorescence parameters. Rising NaCl concentration led to a substantial increase in foliar proline, malondialdehyde and soluble carbohydrates accumulation in ‘P’. On the contrary, ‘R18’, ‘R19’, ‘R23’ and ‘R30’ exhibited a decline in soluble carbohydrates and a significant enhancement in starch under salinity conditions. The water status reflected by midday leaf water potential (ψl) and leaf osmotic potential (ψπ) was significantly affected in ‘P’ and was maintained a stable level in ‘R18’, ‘R19’, ‘R23’ and ‘R30’ under salt stress. The increase in foliar Na+ and Cl− content was more accentuated in parental plants than in regenerated plants. The leaf K+, Ca2+ and Mg2+ content reduction was more aggravated under salt stress in ‘P’. Under increased salt concentration, ‘R18’, ‘R19’, ‘R23’ and ‘R30’ associate lower foliar Na+ content with a higher plant tolerance index (PTI), thus maintaining a normal growth, while foliar Na+ accumulation was more pronounced in ‘P’, revealing their failure in maintaining normal growth under salinity stress. ‘R18’, ‘R19’, ‘R23’ and ‘R30’ showed an obvious salt tolerance by maintaining significantly high chlorophyll content. In ‘R18’, ‘R19’, ‘R23’ and ‘R30’, the enzyme scavenging machinery was more performant in the roots compared to the leaves. Salt stress led to a significant augmentation of catalase, ascorbate peroxidase and guaiacol peroxidase activities in the roots of ‘R18’, ‘R19’, ‘R23’ and ‘R30’. In contrast, enzyme activities were less enhanced in ‘P’, indicating lower efficiency to cope with oxidative stress than in ‘R18’, ‘R19’, ‘R23’ and ‘R30’. ACC deaminase activity was significantly higher in ‘R18’, ‘R19’, ‘R23’ and ‘R30’ than in ‘P’. The present study suggests that regenerated plants ‘R18’, ‘R19’, ‘R23’ and ‘R30’ showed an evident stability in tolerating salinity, which shows their potential to be adopted as interesting selected mutants, providing the desired salt tolerance trait in eggplant.

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

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