scholarly journals Adult Male Rats Show Resilience to Adolescent Bisphenol A Effects on Hormonal and Behavioral Responses While Co-Exposure With Hop Extracts Supports Synergistic Actions

2021 ◽  
Vol 3 ◽  
Author(s):  
Alexandre Morin ◽  
Lise Van de Beeck ◽  
Emmanuelle Person ◽  
Helene Plamondon
Keyword(s):  
Bisphenol A ◽  
Corn Oil ◽  
Forced Swim Test ◽  
Behavioral Responses ◽  
Emotional Behavior ◽  
Male Rats ◽  
Brain Maturation ◽  
Male Wistar Rats ◽  
Adolescence Period ◽  
The Impact

The adolescence period, marked by sexual and brain maturation, has shown sensitivity to various environmental disruptors. Exposure to the xenoestrogen bisphenol A (BPA) is known to alter physiological and behavioral responses although its role at this critical period remains largely unknown. Recent research further suggests biochemical and genomic effects of BPA to be mitigated by various natural compounds, while effects on behavior have not been examined. This study aimed to characterize (1) the effects of dietary BPA during adolescence on endogenous corticosterone (CORT) secretion, emotional behavior, and testosterone (T) in adulthood, and (2) the impact of combined exposure to BPA with hop extracts (Hop), a phytoestrogen with anxiolytic properties. To do so, four groups of male Wistar rats [postnatal day (PND) 28] were administered corn oil (control), BPA (40 mg/kg), hops (40 mg/kg), or BPA-hops by oral gavage for 21 days (PND 28–48). Blood droplets collected on PND 28, 48, and 71 served to measure CORT and T changes. As adults, rats were tested in the elevated plus maze (EPM), the social interaction test, and the forced swim test. Our findings demonstrated elevated anxiety and a trend toward depressive-like behaviors in BPA- compared to hops-exposed rats. However, BPA intake had no impact on basal CORT levels, or adulthood T secretion and sociability. Of note, BPA's anxiogenic effect manifested through decreased EPM open arm entries was abolished by hops co-supplementation. Together, our observations suggest the adolescence period to be less sensitive to deleterious effects of BPA than what has been reported upon gestational and perinatal exposure.

Early postnatal overnutrition impairs VO2max gains with moderate exercise and increase post-exercise muscle damage in adult male rats

2021 ◽  
pp. 1-5
Author(s):  
Douglas Lopes Almeida ◽  
Gabriel Sergio Fabricio ◽  
Laize Peron Tófolo ◽  
Tatiane Aparecida Ribeiro ◽  
Camila Cristina Ianoni Matiusso ◽  
...  
Keyword(s):  
Early Life ◽  
Male Rats ◽  
Moderate Intensity ◽  
Moderate Exercise ◽  
Moderate Intensity Exercise ◽  
Post Exercise ◽  
Reduced Body ◽  
Male Wistar Rats ◽  
The Impact ◽  
Adaptation To Exercise

Abstract Exercise counteracts obesity effects, but information on how early-life obesity may affect long-term adaptation to exercise is lacking. This study investigates the impact of early-life postnatal overfeeding (PO) on animals’ adaptation to exercise. Only male Wistar rats were used. On postnatal day (PN) 30, rats from control (NL-9 pups) or PO (SL-3 pups) litters were separated into four groups: NL-sedentary (NL-Se), NL-exercised (NL-Ex), SL-sedentary (SL-Se), and SL-exercised (SL-Ex). Exercised groups performed moderate-intensity exercise, running on a treadmill, from PN30 to PN90. Further experiments were carried out between PN90 and PN92. PO promoted obesity in SL versus NL rats (P < 0.05). Exercise reduced body weight (P < 0.001), body fat (P < 0.01), and improved glucose homeostasis in SL-Ex versus SL-Se. SL-Ex presented lower VO2max (P < 0.01) and higher post-exercise LDH (P < 0.05) compared to NL-Ex rats. Although moderate exercise counteracted obesity in SL rats, early-life overnutrition restricts fitness gains in adulthood, indicating that early obesity may impair animals’ adaptation to exercise.

Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage

2020 ◽  
Vol 36 (7) ◽  
pp. 502-513
Author(s):  
Işil Aydemir ◽  
Caner Özbey ◽  
Oktay Özkan ◽  
Şadiye Kum ◽  
Mehmet İbrahim Tuğlu
Keyword(s):  
Lymph Node ◽  
Bisphenol A ◽  
Tissue Damage ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Female Rats ◽  
Male Rats ◽  
High Dose ◽  
Organ Function ◽  
Pregnant Rats

Bisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has a steroid hormone-like activity; therefore, it poses a danger to human health. In our study, we aimed to investigate the effects of BPA on lymph node and spleen in male rats exposed to this agent during prenatal stage. The pregnant female rats were divided into four groups: control, sham, low dose (300 µg/kg BPA), and high dose (900 µg/kg BPA). BPA was dissolved in 1 mL of corn oil and administered to the pregnant rats every day during pregnancy. On the 21st and 45th day after the birth, male rats’ lymph node and spleen samples were taken and histopathological examination was performed. Samples were stained with hematoxylin and eosin to determine the general histological appearance, and with CD3 and CD20 immunohistochemically. The results of staining were evaluated by H-score, and statistical analysis was performed. In the samples, BPA applications were not found to cause significant tissue damage. But there was a significant decrease in the immunoreactivities of CD3 and CD20 after BPA applications in both 21st and 45th day samples. After high dose BPA administration, decreased CD3 immunoreactivity was statistically significant. It is thought that BPA does not cause histologically significant tissue damage, but it may impair organ function at cellular level. The investigation of molecules involved in organ function will be useful in revealing the mechanisms that will cause dysfunction.

scholarly journals A paternal methyl donor depleted diet leads to increased anxiety- and depression-like behavior in adult rat offspring

2018 ◽  
Vol 38 (4) ◽  
Author(s):  
Chelsea R. McCoy ◽  
Nateka L. Jackson ◽  
Rachel L. Brewer ◽  
Mohamad M. Moughnyeh ◽  
Daniel L. Smith ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Germ Line ◽  
Emotional Health ◽  
Forced Swim Test ◽  
Emotional Behavior ◽  
Male Rats ◽  
Anxiety And Depression ◽  
Intergenerational Effects ◽  
Methyl Donor ◽  
Adult Rat

Epigenetic mechanisms such as DNA methylation elicit lasting changes in gene expression and likely mediate gene–environment interactions that shape brain development, behavior, and emotional health. Myriad environmental factors influence DNA methylation, including methyl donor content in the paternal diet, could influence methylation in offspring via changes in the paternal germ line. The present study examines the effects of paternal methyl donor dietary deficiency on offspring’s emotional behaviors, including anxiety, social interaction, and depression-like behavior. We previously found that rats bred to display high levels of anxiety- and depression-like behavior exhibit diminished DNA methylation in the amygdala. We also observed that depleting dietary methyl donor content exacerbated the rats’ already high levels of anxiety- and depression-like behavior. Here we sought to determine whether paternal dietary methyl donor depletion elicits intergenerational effects on first generation (F1) offspring’s behavior (potentially triggering a similar increase in anxiety- and/or depression-like behavior). Thus, adult male rats prone to high anxiety/depression-like behavior, were fed either a methyl donor depleted (DEP) or control (CON) diet for 5 weeks prior to mating. They were paired with females and resultant F1 male offspring were subjected to a behavioral test battery in adulthood. F1-DEP offspring showed a similar behavioral profile to the F0 males, including greater depression-like behavior in the forced swim test (FST) and increased anxiety-like behavior in the open field test (OFT). Future work will interrogate molecular changes in the brains of F1 offspring that mediate these intergenerational effects of paternal methyl donor dietary content on offspring emotional behavior.

scholarly journals THE EFFECTS OF KEFIR ON THE INFLAMATORY STATUS AND THYROID FUNCTION (EXPERIMENTAL STUDY ON WISTAR RATS AFTER EXPOSED TO CHLORPYRIFOS)

2016 ◽  
Vol 9 (5) ◽  
pp. 165 ◽  
Author(s):  
Rasipin Rasipin ◽  
Edi Dharmana ◽  
Suharyo Hadisaputro ◽  
Suhartono .
Keyword(s):  
Thyroid Function ◽  
Wistar Rats ◽  
Thyroid Dysfunction ◽  
Corn Oil ◽  
Thyroid Stimulating Hormone ◽  
Male Rats ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
Α Level ◽  
Factor Α

ABSTRACTObjective: Goiter is an enlarged thyroid gland remained a health problem in the agricultural areas. Chlorpyrifos (CPF) is a pesticide widely used byfarmers. Previous studies proved that CPF exposure caused thyroid dysfunction. The objective of this study was to evaluate the effects of kefir on theinflammatory status and thyroid function in male Wistar rats after exposed to CPF using biochemical and histopathological assays.Methods: Male rats were divided into 4 groups, i.e., CPF 5+kefir (5 mg/kg+3.6 ml/200 g, respectively), CPF 5 (5 mg/kg), corn oil (CO 1 ml/200 g), andnegative control (NC: Without CPF, CO, and kefir).Results: Kefir supplementation dose 3.6 ml/200 g once a day for 28 days in the rats after exposed to CPF dose 5 mg/kg once a day for 14 days, inCPF 5+kefir as compared to CPF 5: Significantly (p<0.05) decreased serum tumor necrosis factor-α (TNF-α) level; significantly (p<0.01) maintainedserum levels of tumor growth factor-β (TGF-β) and thyroid stimulating hormone (TSH) not to decrease; not significant (p>0.05) decreased the level ofinterleukin-1β, cluster of differentiation-26 expression and level of T serum; not significant (p>0.05) maintained the level of anti-thyroid peroxidasenot to decrease; and not significant (p>0.05) increased the apoptosis index. This study suggests that CPF exposure causes the inflammatory processwhich leads to thyroid dysfunction.4Conclusion: Kefir supplementation significantly decreased the level of TNF-α and maintained the levels of TGF-β and TSH not to decrease, possible toreduce the inflammatory and thyroid dysfunction processes caused by exposure to CPF in experimental animals.Keywords: Kefir, Chlorpyrifos, Inflammation, Thyroid function. 

scholarly journals Induction Effect of Bisphenol A on Gene Expression Involving Hepatic Oxidative Stress in Rat

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Sohrab Kazemi ◽  
Seydeh Narges Mousavi ◽  
Fahimeh Aghapour ◽  
Boshra Rezaee ◽  
Farzin Sadeghi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Bisphenol A ◽  
Corn Oil ◽  
Rna Extraction ◽  
Control Group ◽  
Induction Effect ◽  
Hepatic Oxidative Stress ◽  
Body Weights ◽  
Male Wistar Rats

Background and Objective.Bisphenol A (BPA) is an abundantly used xenoestrogenic chemical which may cause various disorders in body. In the present study, we sought to investigate the effects of various doses of BPA on hepatic oxidative stress-related gene expression in rats.Methods.Male Wistar rats weighing 150–200 g were used in this study. Three doses of the BPA (5, 25, and 125 μg/kg) in corn oil were administered as gavage during 35 consecutive days. After the experiment, the rats were expired and the livers were removed and stored at −80°C freezer for RNA extraction.Findings.The Real Time PCR showed increased expression of HO-1 in the rats receiving BPA doses compared to the control group. This effect was dose-dependent and higher at doses of 25 and 125 μg/kg than 5 μg/kg of body weight (p<0.05). It was also demonstrated that various doses BPA can increase GADD45B gene expression compared to control group. That expression was significantly dominant in the lowest dose (5 μg/kg) of the BPA (p<0.05). The final body weights (168.0±10.0 gr) in the treatment group [BPA (125 μg/kg)] showed a significant decrease compared to control group (191.60±6.50 gr).Conclusion.These findings demonstrate that BPA generated ROS and increased the antioxidant gene expression that causes hepatotoxicity.

Neuroprotective role of 6-Gingerol-rich fraction of Zingiber officinale (Ginger) against acrylonitrile-induced neurotoxicity in male Wistar rats

2018 ◽  
Vol 30 (3) ◽  
Author(s):  
Ebenezer Olatunde Farombi ◽  
Amos Olalekan Abolaji ◽  
Babatunde Oluwafemi Adetuyi ◽  
Olaide Awosanya ◽  
Mobolaji Fabusoro
Keyword(s):  
Brain Damage ◽  
Corn Oil ◽  
Zingiber Officinale ◽  
Protective Role ◽  
Male Rats ◽  
Cortex Lesion ◽  
Male Wistar Rats ◽  
Group B ◽  
Factor Α

Abstract Background Acrylonitrile (AN) is a neurotoxin that is widely used to manufacture synthetic fibres, plastics and beverage containers. Recently, we reported the ameliorative role of 6-gingerol-rich fraction from Zingiber officinale (Ginger, GRF) on the chlorpyrifos-induced toxicity in rats. Here, we investigated the protective role of GRF on AN-induced brain damage in male rats. Methods Male rats were orally treated with corn oil (2 mL/kg, control), AN (50 mg/kg, Group B), GRF (200 mg/kg, Group C), AN [50 mg/kg+GRF (100 mg/kg) Group D], AN [(50 mg/kg)+GRF (200 mg/kg) Group E] and AN [(50 mg/kg)+N-acetylcysteine (AC, 50 mg/kg) Group F] for 14 days. Then, we assessed the selected markers of oxidative damage, antioxidant status and inflammation in the brain of rats. Results The results indicated that GRF restored the AN-induced elevations of brain malondialdehyde (MDA), interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) and Nitric Oxide (NO) levels. GRF also prevented the AN-induced depletion of brain glutathione (GSH) level and the activities of Glutathione S-transferase (GST), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in rats (p<0.05). Furthermore, GRF prevented the AN-induced cerebral cortex lesion and increased brain immunohistochemical expressions of Caspases-9 and -3. Conclusions Our data suggest that GRF may be a potential therapeutic agent in the treatment of AN-induced model of brain damage.

scholarly journals Impact of Buprenorphine on Learning and Memory Ability Related to the Acetylcholinesterase Change in the Hippocampus of Rats

2021 ◽  
Vol 11 (5) ◽  
pp. 13111-13114
Keyword(s):  
Memory Impairment ◽  
Ache Activity ◽  
Memory Function ◽  
Male Rats ◽  
Control Groups ◽  
Treatment Groups ◽  
Activity Inhibition ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Impact

Buprenorphine (BUP), a “synthetic opioid”, may cause memory impairment. This investigation aimed to study the impact of BUP on memory function related to acetylcholinesterase (AChE) activity inhibition in male rats. 24 male Wistar rats were randomly divided into three groups; control (C) and two treatment groups BUP (0.3 and 1) (n=8, for each group). BUP (0.3 and 1 mg/kg) was administrated subcutaneously once a day for 30 days. Normal saline 0.9% was injected in to control groups. In the end, animals were anesthetized and decapitated, and their hippocampus was dissected to assess AChE activity. There were no significant differences between the activities of AChE in the hippocampus in BUP-treated animals compared with controls. Besides, the activities of AChE in the BUP 0.3 group and BUP 1 group did not indicate a significant difference. These findings did not confirm the effect of BUP at doses of 0.3 and 1 mg/kg on memory function associated with the AChE activity inhibition.

scholarly journals Effects of Buprenorphine on the Memory and Learning Deficit Induced by Methamphetamine Administration in Male Rats

2021 ◽  
Vol 15 ◽  
Author(s):  
Farshid Etaee ◽  
Arezoo Rezvani-Kamran ◽  
Somayeh Komaki ◽  
Masoumeh Asadbegi ◽  
Nafiseh Faraji ◽  
...  
Keyword(s):  
Learning And Memory ◽  
Open Field ◽  
Spatial Learning ◽  
Avoidance Learning ◽  
Field Tests ◽  
Male Rats ◽  
Spatial Learning And Memory ◽  
Memory And Learning ◽  
Male Wistar Rats ◽  
The Impact

Little is known about the effects of methamphetamine (Meth) and buprenorphine (Bup) on memory and learning in rats. The aim of this investigation was to examine the impact of Meth and Bup on memory and learning. Fourteen male Wistar rats weighing 250–300 g were assigned to four groups: Sham, Meth, Bup, and Meth + Bup and were treated for 1 week. Spatial learning and memory, avoidance learning, and locomotion were assessed using the Morris water maze, passive avoidance learning, and open field tests, respectively. Meth and Bup impaired spatial learning and memory in rats. Co-administration of Meth + Bup did not increase the time spent in the target quadrant compared to Meth alone in the MWM. The Bup and Meh + Bup groups were found with an increase in step-through latency (STLr) and a decrease in the time spent in the dark compartment (TDC). Meth and Bup had no effects on locomotor activity in the open field test. Bup showed a beneficial effect on aversive memory. Since Bup demonstrates fewer side effects than other opioid drugs, it may be preferable for the treatment of avoidance memory deficits in patients with Meth addiction.

scholarly journals Gallic Acid Ameliorates Bisphenol A-Induced Toxicity in Wistar Rats

2018 ◽  
Vol 12 (4) ◽  
pp. 11-18
Author(s):  
Eunice Olufunke Ola-Davies ◽  
◽  
Samuel Gbadebo Olukole ◽  
Damilare Olaniyi Lanipekun ◽  
◽  
...  
Keyword(s):  
Bisphenol A ◽  
Gallic Acid ◽  
Adult Male ◽  
Wistar Rats ◽  
Corn Oil ◽  
Density Lipoprotein ◽  
Concomitant Treatment ◽  
Research Information ◽  
Male Wistar Rats ◽  
Liver And Kidney

Background: Bisphenol A (BPA) has received attention in environmental and toxicological research due to its widespread effects in biological systems. While several anti-oxidants have been used in ameliorating BPA-induced toxicities in experimental animals, there is the scarcity of research information on the use of Gallic acid (GA) in protecting against BPA-induced toxicity. This study investigated the ameliorative effect of Gallic acid in BPA-induced toxicities of the adult male Wistar rats. Methods: Thirty two adult male Wistar rats were randomly assigned into four groups of eight animals each as follows: Group 1 (Control rats): 0.2 ml of corn oil; Group 2 (GA-treated rats): 20 mg/kg/day GA (dissolved in distilled water); Group 3 (BPA-treated rats): 10 mg/kg/day BPA suspended in 0.2 ml corn oil; Group 4 (BPA+GA-treated rats): BPA (10 mg/kg/day) with a concomitant GA (20 mg/kg/day). All treatments were orally administered for 14 days. Results: BPA significantly increased (P<0.05) in the values of liver function enzymes (ALP, AST, ALT, GGT), total globulin, conjugated globulin, triglycerides, total cholesterol, low-density lipoprotein, creatinine, and urea as well as sodium ions. Concomitant treatment with GA ameliorated these elevated values. Moreover, BPA-induced histopathological alterations in the liver and kidney while GA ameliorated them. Conclusion: BPA caused structural and cellular perturbations of the blood, liver, and kidney of rats while concomitant treatment with GA ameliorates the condition. Hence, GA has hepato-protective and nephroprotective actions against BPA-induced toxicity in Wistar rats.

Perinatal exposure to a low dose of bisphenol-A advances pubertal spermatogenesis of F1 adolescent male rats

2020 ◽  
Author(s):  
Yinyang Bai ◽  
Fang Xiong ◽  
Yun Zhang ◽  
Jie Chen ◽  
Lishuang Xu ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Plasma Levels ◽  
Low Dose ◽  
Male Offspring ◽  
Female Rats ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Perinatal Exposure ◽  
Significant Difference ◽  
The Impact

Abstract Background To investigate the impact of perinatal exposure to a low dose of bisphenol A (BPA) on spermatogenesis in male rats and the underlying mechanism. Methods Female rats were injected subcutaneously with 2 µg BPA/kg/day from gestation day 10 through lactation day 7. The spermatogenesis and expression of key regulatory genes in the testes as well as the central modulators of the hypothalamic-pituitary-gonadal axis were determined in male offspring on postnatal day 18, 21, and 24 (PND18, 21, and 24). Results 1) Perinatal BPA exposure led to an increase in the weight of body and testis in PND21-24 male offspring. The seminiferous tubular diameter and the number of round spermatids were significantly increased in PND21 BPA-rats, while the volumes of the Sertoli cells, spermatogonia and spermatocytes were not significantly altered. 2) Compared to the control rats, the expression levels of key meiotic regulators such as cyclinA1, c-jun and c-fos in the seminiferous tubules were significantly elevated in PND21 BPA-rats. 3) The plasma levels of FSH and LH (PND21 and PND24) as well as the frequency of pulsatile LH secretion (PND21) were significantly increased in BPA-rats, although the plasma levels of testosterone and estrogen showed no significant difference between the two groups. 4) In comparison with control rats, the levels of GnRH mRNA in the preoptic area (POA) and kiss1 mRNA in arcuate nucleus (ARC) were significantly increased in the BPA-rats, whereas the level of ERα mRNA in ARC was decreased, although the number of GnRH-positive cells and ARC kisspeptin-positive cells were unchanged. Interestingly, neither the number of kisspeptin-positive cells nor the level of kiss1 mRNA in the anteroventral periventricular nucleus (AVPV) showed a difference between the two groups. Conclusion Perinatal exposed to a low dose of BPA leads to an increased meiosis of spermatocytes and promotes the spermatogenesis in male offspring, most likely through activation of the hypothalamic-pituitary-gonadal axis.

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