Effects of Purple Corn Anthocyanin on Blood Biochemical Indexes, Ruminal Fluid Fermentation, and Rumen Microbiota in Goats

The objective of this study was to observe the effects of anthocyanin from purple corn on blood biochemical indexes, ruminal fluid fermentation parameters, and the microbial population in goats. A total of 18 Qianbei Ma wether kids (body weight, 21.38 ± 1.61 kg; mean ± standard deviation) were randomly assigned to three groups using a completely randomized design. The group diets were: (1) control, basal diet, (2) treatment 1 (LA), basal diet with 0.5-g/d purple corn pigment (PCP), and (3) treatment 2 (HA), basal diet with 1-g/d PCP. The results showed that supplementation of PCP anthocyanin increased (P < 0.05) crude protein and gross energy digestibilities compared to the control. Compared to the control group, the inclusion of anthocyanin-rich PCP led to significantly increased (P < 0.05) plasma reduced glutathione and peroxidase concentrations. Goats receiving PCP had increased (P < 0.05) ruminal fluid acetic acid and a higher ratio of acetate to propionate, while the propionic acid, butyric acid, valeric acid, isobutyric acid, and isovaleric acid levels had decreased (P < 0.05). There was no significant difference (P > 0.05) in ruminal fluid alpha bacterial diversity among the three groups. At the phylum level, the feeding of PCP had significant effect (P < 0.05) on the abundances of Actinobacteriota, Proteobacteria, Elusimicrobiota, WPS-2, and Cyanobacteria. At the genus level, HA group had lower (P < 0.05) Prevotellaceae_NK3B31_group abundance compared to the other groups. In addition, significant differences (P < 0.05) were also observed for the ruminal fluid Eubacterium_nodatum_group, Amnipila, Ruminiclostridium, U29-B03, unclassified_c_Clostridia, Pyramidobacter, Anaeroplasma, UCG-004, Atopobium, norank_f_norank_o_Bradymonadales, Elusimicrobium, norank_f_norank_o_norank_c_norank_p_WPS-2, norank_f_Bacteroidales_UCG-001, and norank_f_norank_o_Gastranaerophilales among all groups. Taken together, the inclusion of anthocyanin-rich PCP increased the antioxidant potential, improved rumen volatile fatty acids, and induced a shift in the structure and relative abundance of ruminal microbiota in growing goats.

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Effects of Dietary Chicory (Chicorium intybus L.) and Probiotic Blend as Natural Feed Additives on Performance Traits, Blood Biochemistry, and Gut Microbiota of Broiler Chickens

Antibiotics ◽

10.3390/antibiotics9010005 ◽

2019 ◽

Vol 9 (1) ◽

pp. 5 ◽

Cited By ~ 5

Author(s):

Mohammadreza Khoobani ◽

Seyyed-Hamed Hasheminezhad ◽

Faramin Javandel ◽

Mehran Nosrati ◽

Alireza Seidavi ◽

...

Keyword(s):

Gut Microbiota ◽

Broiler Chickens ◽

Body Weight Gain ◽

Basal Diet ◽

Feed Additives ◽

The Body ◽

The Other ◽

Control Group ◽

Blood Biochemical ◽

Randomized Design

The experiment was designed to determine the effect of different levels of chicory (Chicorium intybus L.) powder and a probiotic blend (PrimaLac®) on productive performance, blood biochemical parameters, and ileal microbiota in broiler chickens. A total of 225 one-day-old broilers (Ross 308) were used in a completely randomized design with five experimental diets as follows: 1—basal-diet without supplements (control-group); 2—basal-diet including probiotic blend; 3— basal-diet including 0.10% chicory; 4—basal-diet including 0.15% chicory; 5—basal-diet including 0.20% chicory. At 42 days of age, representative birds per replicate were randomly selected for blood samples and carcass measurements. Results showed that the body weight gain of broilers fed the probiotic blend or 0.10% chicory was significantly (P < 0.05) higher than those fed on the other treatments. The abdominal fat pad was significantly (P < 0.05) lower in birds fed diets including chicory compared with control or probiotic. Blood triglycerides and LDL levels were reduced (P < 0.05) and HDL increased (P < 0.05) when fed probiotic or chicory whereas no significant effect on the other serum parameters was found. Broiler ileal microflora from the control group had significantly (P < 0.05) higher count of E. coli and lower Lactobacillus than those from the other groups. From findings, it is possible to conclude that dietary chicory powder supported positively growth performance and improved gut microbiota in broiler chickens. However, more research is needed on this subject to better understand the mode of action of feed additives used.

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Effect of royal jelly on milk composition and blood biochemical parameters in lactating ewes

The Journal of Agricultural Science ◽

10.1017/s0021859619000352 ◽

2019 ◽

Vol 157 (2) ◽

pp. 176-181

Author(s):

Mahmoud S. El-Tarabany ◽

Akram A. El-Tarabany ◽

Mostafa A. Atta ◽

Omar A. Ahmed-Farid ◽

Mohamed M. Mostafa

Keyword(s):

Milk Fat ◽

Nutritive Value ◽

Royal Jelly ◽

Antioxidant Activities ◽

Basal Diet ◽

Milk Composition ◽

Feed Additives ◽

Control Treatment ◽

Control Group ◽

Blood Biochemical

AbstractUse of antibiotics as feed additives has been reduced to avoid the hazard of drug residues, and consequently, the search for alternative natural additives has developed. Thus, the aim was to evaluate the influence of royal jelly (RJ) supplementation on milk composition, blood biochemical and antioxidant parameters of lactating ewes. Thirty-six Ossimi ewes were divided randomly into two groups (18 animals each). For a period of 4 weeks, the control group (CON) was fed a basal diet only, while the other group was fed the basal diet and supplemented with a single bolus of RJ (1000 mg/head). The RJ-supplemented ewes produced significantly higher milk protein, fat and total solids than the CON group. The RJ group had a significantly higher red blood cell count, haemoglobin content, haematocrit value and total leucocyte counts, but lower neutrophil to lymphocyte ratio when compared with the control treatment. The RJ group showed significantly higher concentrations of total antioxidant capacity, superoxide dismutase activity and glutathione in the serum compared with the control treatment. In conclusion, RJ supplements can improve the nutritive value of milk fat and the serum antioxidant activities in lactating ewes.

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Hepatic Histomorphological Changes and Oxidative Stress Profile of Clarias gariepinus (Burchell, 1822) Juveniles Fed with Sodium Propanoate-Preserved Diets

Notulae Scientia Biologicae ◽

10.15835/nsb10410316 ◽

2018 ◽

Vol 10 (4) ◽

pp. 460-465

Author(s):

Patrick E. ABA ◽

Ifeanyi E. UZOCHUKWU ◽

Nelson I. OSSAI ◽

Ifeanyi G. EKE

Keyword(s):

Oxidative Stress ◽

Biological Effects ◽

Clarias Gariepinus ◽

Basal Diet ◽

Serum Levels ◽

Control Group ◽

Stress Profile ◽

Fish Feed ◽

Inflammatory Parameters ◽

Significant Difference

Sodium propanoate is in the list of approved feed preservatives. However, there is dearth of information on its biological effects on the C. gariepinus. The present study investigated the effect of sodium propanoate-preserved feed on the hepatic histomorphometric changes, oxidative stress and inflammatory parameters of C. gariepinus juveniles. One hundred juveniles of mixed sexes, assigned into 5 groups of 20 fish per group, with each group consisting of 2 replicates of 10 fish, were used for the investigation. Group A juveniles were fed basal diet, while groups B-E received basal diet incorporated with sodium propanoate at the rate of 25, 50, 75 and 100 g/15 kg of feed respectively. Treatments were done two times daily for 8 weeks. Samples (sera and liver) were collected on the last day for evaluation of a few biochemical parameters (malondialdehyde values, catalase activity, C-reactive protein levels) and histomorphometric alterations in the liver. Results indicated that fish in groups D and E had higher catalase activities, lower serum levels of C-reactive proteins and an intact hepatic histomorphormetry when compared with the control group. There was no significant difference in the plasma malondialdehyde values in all the groups. It was concluded that preservation of fish feed with sodium propanoates improved antioxidant status of C. gariepinus and protected liver histology.

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Effects of different doses of probiotic yeast Saccharomyces cerevisiae on the duodenal mucosa in rabbits

Indian Journal of Animal Research ◽

10.18805/ijar.5570 ◽

2015 ◽

Author(s):

Nilay Seyidoglu ◽

Sabire Peker

Keyword(s):

Saccharomyces Cerevisiae ◽

Basal Diet ◽

Morphological Properties ◽

Control Group ◽

Yeast Culture ◽

Total Thickness ◽

Intestinal Health ◽

Yeast Saccharomyces Cerevisiae ◽

Significant Difference ◽

Different Doses

Probiotics and their components have been used to improve growth performance and immunity, as well as intestinal health. This study evaluated the effect of different doses of Saccharomyces cerevisiae on the morphological properties of duodenums of rabbits. Twenty 6-7 weeks old male New Zealand White Rabbits were randomly allocated into three groups for 90 days. The first group (control group) received the basal diet, the second group received basal diet supplemented with S.cerevisiae at a level of 2g/kg of feed, and the third group was fed with S.cerevisiae live yeast culture added at 4.0 g/kg. At the end of the experiment duodenum segments were taken, fixed in 10% neutral buffered formalin and processed for histological examination. In this study, the total thickness of the mucosa, the height of the villi and depth of the crypts and depth of the glands of the duodenum were found to be longer with the increased yeast doses. However, there was no significant difference among the villus crypt ratio of the groups. In conclusion, the total thickness of the mucosa, villus heights, crypt depths and gland depths were increased significantly in both of the yeast groups of rabbits. Therefore, it may be proposed that administration of S.cerevisiae in either low or high doses may be used for intestinal health.

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BIOCHEMICAL AND HAEMATOLOGICAL CHANGES ASSOCIATED WITH TRANSPLACENTAL CONGENITAL MALARIA IN KOGI STATE, NORTH CENTRAL NIGERIA

10.1101/2021.07.29.21261315 ◽

2021 ◽

Author(s):

Shedrack Egbunu Akor ◽

Dickson Achimugu Musa ◽

Akogu SPO

Keyword(s):

Cord Blood ◽

Statistical Significance ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Blood Biochemical ◽

Significant Difference ◽

Haematological Changes ◽

Haematological Indices ◽

Congenital Malaria

Background: Transplacental congenital malaria is a vertical transplacental transmission of malaria parasites from the mother to the baby in utero or perinatally during labor. Cord blood that conveyed oxygen and nutrients from mother to fetus and return with carbon dioxide and other waste materials can transmit malaria pathogen. This study is aim to establish early diagnosis of transplacental congenital malaria using cord blood biochemical and haematological indices. Cord blood from 164 babies delivered at three hospitals in Kogi State between January and December, 2020 were microscopically investigated for malaria parasite. Biochemical and Haematological analyses were done using SYSMEX XP 300, Roche 9180 and VIS Spectrophotometer model 721. The data obtained were expressed as mean plus or minus standard deviation using SPSS 23. The indicator level of statistical significance was set at p<0.05. The results showed significant (p<0.05) decreased in values of WBC, platelet, sodium, potassium, urea, creatinine, RBC, PCV, haemoglobin and MCHC in malaria infected cord blood in comparison to malaria negative control group. Significant (P<0.05) increased activities of liver enzymes (AST, ALT, ALP), total protein, bicarbonate and chloride in malaria infected cord blood when compared with malaria negative group. However, no statistically significant difference in lymphocyte, MCV, MCH, neutrophil and mixed of both malaria infected and malaria negative cord blood. This study suggests that cord blood biochemical and haematological indices can be used to diagnose and manage transplacental congenital malaria in fetus and neonates. Keywords: Transplacental, Biochemical, Haematological and Congenital Malaria.

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Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan

Journal of Basic Medical Veterinary ◽

10.20473/jbmv.v9i2.28586 ◽

2021 ◽

Vol 9 (2) ◽

pp. 79

Author(s):

Febriyono Anggela Prakoso ◽

Raden Tatang Santanu Adikara ◽

Kusnoto Kusnoto ◽

Sri Hidanah ◽

Yeni Dhamayanti ◽

...

Keyword(s):

Growth Rate ◽

Weight Gain ◽

Research Methods ◽

Control Group ◽

Treatment Groups ◽

Gain Rate ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Bali Cattle

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.

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Supplementation With Purple Corn Anthocyanin Improves Growth Performance, Meat Quality, Muscle Antioxidant Status and Unsaturated Fatty Acid Profiles in Growing Goat

10.21203/rs.3.rs-359148/v1 ◽

2021 ◽

Author(s):

Xingzhou Tian ◽

Jiaxuan Li ◽

Di Zhou ◽

Qingyuan Luo ◽

Xu Wang ◽

...

Keyword(s):

Fatty Acid ◽

Growth Performance ◽

Meat Quality ◽

Antioxidant Status ◽

Basal Diet ◽

Fatty Acid Profiles ◽

Dpph Scavenging Activity ◽

Purple Corn ◽

Dpph Scavenging ◽

Growing Goats

Abstract Background: There are abundant anthocyanin compounds in purple corn, which has a wide range of biological activity in terms of antioxidation, scavenging free radicals, and preventing lipid peroxidation. However, the impact of anthocyanins on the meat quality, muscle antioxidant activity and fatty acid profiles in growing goats has not been studied. This experiment was conducted to observe the effects of anthocyanin from purple corn pigment (PCP) on growth performance, nutrient digestibility, meat quality, muscle antioxidant activity and fatty acid profiles of goats during the growing period. It aimed to provide the important rationale for anthocyanin preventing lipid peroxidation in goats.Results: A total of eighteen Qianbei Ma wether kids (body weight, 21.38 ± 1.61 kg; mean ± standard deviation) were randomly allotted into three groups using a completely randomized design with six duplicates per group. The dietary treatments were: 1) the control group was provided with basal diet; 2) treatment 1 (LA) was provided basal diet with 0.5 g/d PCP; and 3) treatment 2 (HA) was provided basal diet with 1 g/d PCP. The results showed that supplementation of PCP anthocyanin could increase (P < 0.05) CP and GE digestibilities compared to the control. The addition of PCP tended to drop (P < 0.05) shear force during the growing goat phase. Goat receiving PCP showed higher (P < 0.05) levels of reduced glutathione, DPPH scavenging activity, and peroxidase in Longissimus dorsi muscle (LD) relative to the control. Compared with the control, LA and HA displayed lower (P < 0.05) concentrations C10:0, C12:0, C14:0, C15:0, C16:0, C17:0 and saturated fatty acid (SFA), but increased (P < 0.05) various individual unsaturated FA of C14:1, C16:1, C17:1, C18:1n9, C18:1n12, C18:2n6, C18:3n3, C20:4n6, C22:1n9, C22:4, C22:5n6, C22:6n3, C24:1, PUFA, n-6 PUFA, MUFA/SFA and PUFA/SFA. Specifically, some stronger (P < 0.05) positive correlations were detected between antioxidant status and UFA profiles (GSH-Px, GSH, catalase, DPPH scavenging activity with C14:1, C15:1, C16:1, C18:1n9, C20:4n6, C22:1n9, C22:4, C22:5n6, C24:1, MUFA) in LD of goats.Conclusion: Results indicated that anthocyanin could be used as a source of natural functional additive because anthocyanin-rich PCP had the potential to improve growth performance, meat quality, and enhance muscle antioxidant status and unsaturated FA profiles in the growing goats.

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Influence of Bacterial Organic Selenium on Blood Parameters, Immune Response, Selenium Retention and Intestinal Morphology of Broiler Chickens

10.21203/rs.2.23476/v2 ◽

2020 ◽

Author(s):

A. M. Dalia ◽

T. C. Loh ◽

A. Q. Sazili ◽

Anjas Asmara Samsudin

Keyword(s):

Broiler Chickens ◽

Hematological Parameters ◽

Basal Diet ◽

Blood Parameters ◽

Intestinal Morphology ◽

Control Group ◽

Villus Height ◽

Organic Selenium ◽

Significant Difference ◽

Immunity Response

Abstract Background: Several studies indicated that dietary organic selenium (Se) usually absorbed better than an inorganic source, with high retention and bioavailability. Dietary Se as an antioxidant element affects the immune system and hematological status in animals. Therefore, the aim of this study was to evaluate the effect of dietary supplementation of bacterial selenium as an organic source on hematology, immunity response, selenium retention, and gut morphology in broiler chickens. Results: The present results revealed that supplementation of inorganic Se was associated with the lowest level of RBC, HB, and PCV with significant difference than ADS18-Se. In the starter stage, both T2 and T5 were associated with the significantly highest IgG level compared to the basal diet, while all supplemented groups showed higher IgM levels compared to the control group. In the finisher phase, all Se supplemented groups showed significant (P ˂ 0.05) increases in IgG, IgA, and IgM levels compared to T1. Birds fed bacterial-Se showed high intestinal villus height and better Se retention more than sodium selenite. The organic selenium of ADS18 had a superior action in improving Se retention compared to ADS1 and ADS2 bacterial Se. Conclusion: Bacterial organic Se had a beneficial effect on the villus height of small intestine led to high Se absorption and retention. Thus, it caused a better effect of Se on hematological parameters and immunity response.

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Effect of Adding Different Levels of Portulaca oleracea L. Seeds and Leaves Powder to The Diet on Productive and Physiological Performance of Broiler Chickens (Ross 308)

Basrah Journal of Agricultural Sciences ◽

10.37077/25200860.2021.34.1.04 ◽

2021 ◽

Vol 34 (1) ◽

pp. 38-48

Author(s):

Ahmed J. Shirshaab ◽

Jaffar M. Jassim

Keyword(s):

Broiler Chickens ◽

Basal Diet ◽

Serum Glucose ◽

Portulaca Oleracea ◽

Control Group ◽

Broiler Chicks ◽

Positive Role ◽

Randomized Design ◽

Escherichia Coli Bacteria ◽

Different Levels

This study was conducted in the Poultry Field of the College of Agriculture, Basrah University for the period from 20/9/2019 to 24/10/2019 to investigate the effect of adding different levels of Portulaca oleracea L. seeds and leaves powder on some physiological characteristics of broilers. A total 216 one day old unsexed Ross-308 broiler chicks were used in this study. The chicks were randomly distributed into six treatments replicated three times (12 chicks each replicate). The experiment was designed as a completely randomized design (CRD). The first treatment was control (basal diet), P. oleracea seed powder was added as 5 & 10 g.kg-1 for the 2nd and 3rd treatments. Leaves powder was added as 5 and 10 g.kg-1 to the basal diet in treatment 4th and 5th. In the 6th treatment 5 gm of seed and 5 gm of leaves powder was added to the basal diet. The results showed that there was a significant (P <0.05) decrease in serum glucose, cholesterol and triglyceride concentrations in all additive treatments compared to control. There was also a significant decrease (P <0.05) in Escherichia coli bacteria count, as well as a significant improvement (P <0.05) in the number of Lactobacilli bacteria in all addition treatments compared to the control group. The addition of P. oleracea seeds and leaves powder had no significant effect on some haematological indices and on the total protein concentration in the serum. The study confirms the importance of adding powder of seeds or leaves in the broiler diet because it has a positive role in improving some of the physiological and microbial blood characteristics of the broiler.

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Effect of lime treated olive cake on nutrient ulilization and performance of male goats

Indian Journal of Animal Research ◽

10.18805/ijar.7079 ◽

2015 ◽

Author(s):

A. Ishfaq ◽

R. K. Sharma ◽

A. Rastogi ◽

B. A. Malla ◽

S. A. Rather

Keyword(s):

Organic Matter ◽

Adult Male ◽

Nutrient Digestibility ◽

Crude Fiber ◽

Control Group ◽

Olive Cake ◽

Acid Detergent Fiber ◽

Blood Biochemical ◽

Slaked Lime ◽

Significant Difference

Current study was conducted to evaluate the effect of inclusion of lime treated olive cake in the complete feed of goats. Olive cake was treated with 6% slaked lime to increase availability of cellulose and to alleviate digestibility depression caused by higher fat content. Twelve local adult male goats were randomly allotted to control and treatment groups. Control group was fed ad libitum conventional complete feed and the treatment group was fed complete feed containing 30% lime treated olive cake for a duration of 30 days. The treatment feed was significantly (P<0.05) lower in organic matter, crude fiber and acid detergent fiber, however it was significantly (P<0.01) higher in calcium and acid insoluble ash. Mean body weight and daily feed dry matter and organic matter intake was comparable among groups and periods. The nutrient digestibility for crude fiber and acid detergent fiber was significantly (P<0.05) in treatment feed. Daily nitrogen intake and digestible crude protein intake (g/kg W0.75), was comparable between both groups. Calcium and phosphorus balance was comparable between two groups. No significant difference was observed between groups and periods for blood biochemical and serum enzymes. The results of the present study indicated that lime treated olive cake can be included in complete feed at 30% level for feeding of adult male goats without adverse impact on nutrient intake, utilization and general performance of the animal.

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