scholarly journals The Beneficial Effect of Eco-Friendly Green Nanoparticles Using Garcinia mangostana Peel Extract against Pathogenicity of Listeria monocytogenes in Female BALB/c Mice

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 573
Author(s):  
Afrah F. Alkhuriji ◽  
Nada A. Majrashi ◽  
Suliman Alomar ◽  
Manal F. El-Khadragy ◽  
Manal A. Awad ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Listeria Monocytogenes ◽  
Color Change ◽  
Antioxidant Activities ◽  
Inflammatory Factors ◽  
Antioxidant Enzyme Activities ◽  
Psychrophilic Bacterium ◽  
Garcinia Mangostana ◽  
Ag Nps ◽  
Peel Extract

Listeria monocytogenes is a psychrophilic bacterium, which causes widespread zoonosis in the natural environment, and mainly affects goat, sheep, and cattle herds. Recently, we predicted that it can be transmitted through food. It causes listeriosis, a severe infectious disease, which occurs with food contaminated with the pathogenic bacterium. Anti-inflammatory factors are important to treat the dangers of chronic inflammation associated with chronic diseases. Natural foodstuffs have made and are continuing to make vital contributions to the search for new antilisterial agents. The use of natural products in association with silver nanoparticles has drawn attention because of its easy, nonpathogenic, eco-friendly, and economical protocol. Hence, we aimed to biosynthesize silver nanoparticles (Ag-NPs) using Garcinia mangostana peel extract, which was found to be a good source for the synthesis of silver nanoparticles, their formation being confirmed by color change and stability in solution, and investigated the antilisterial activity of these nanoparticles in a murine model of L. monocytogenes infection. A total of 28 mice were divided into four groups—healthy control, infected, infected mice treated with green Ag-NPs biosynthesized with G. mangostana (5 mg/mL), and infected mice pretreated with Ag-NPs. From our results, oral treatment with Ag-NPs biosynthesized with G. mangostana peel extract resulted in a significant reduction in malondialdehyde (MDA), enhanced antioxidant enzyme activities, and increased the levels of the antiapoptotic protein, compared with the untreated mice. These results indicate that G. mangostana may provide therapeutic value against L. monocytogenes-induced oxidative stress and histopathological alterations, and that these effects may be related to antiapoptotic and antioxidant activities.

scholarly journals The effects of silver nanoparticles biosynthesized using fig and olive extracts on cutaneous leishmaniasis-induced inflammation in female balb/c mice

2020 ◽  
Vol 40 (12) ◽  
Author(s):  
Mina A. Almayouf ◽  
Manal El-khadragy ◽  
Manal A. Awad ◽  
Ebtesam M. Alolayan
Keyword(s):  
Silver Nanoparticles ◽  
Protective Effect ◽  
Cutaneous Leishmaniasis ◽  
Leishmania Major ◽  
Antioxidant Activities ◽  
Antileishmanial Activity ◽  
Neglected Diseases ◽  
Parasitic Diseases ◽  
Antioxidant Enzyme Activities ◽  
Ag Nps

Abstract Leishmaniasis is a group of infectious and noncontagious severe parasitic diseases, caused by protozoans of the Leishmania genus. Natural products characterize a rich source of prospective chemical entities for the development of new effective drugs for neglected diseases. Scientific evaluation of medicinal plants has made it possible to use some metabolites from flavonoids and polyphenols compounds for the treatment of parasitic diseases. Therefore, we aimed in the present study to evaluate the protective effect of silver nanoparticles (Ag-NPs) biosynthesized using Fig and Olive extracts (NFO) against Cutaneous leishmaniasis in female Balb/c mice. A total of 70 mice were used and divided into seven groups. Treatment was initiated when local lesions were apparent, we found that Fig and Olive extracts were found to be a good source for the synthesis of (Ag-NPs), their formation was confirmed by color change and stability in solution. Nanoparticles biosynthesized using Fig and Olive extracts induced a reduction in the average size of cutaneous leishmaniasis lesions compared with the untreated mice. Moreover, nanoparticles treatment decreased oxidative stress (LPO, NO), down-regulation gene expression levels (TNF-α, IL-1β, and BAX), and this antileishmanial activity of nanoparticles was associated with enhanced antioxidant enzyme activities. In addition, histopathological evaluation proved the antileishmanial activity of nanoparticles compared with the positive control. Therefore, we aimed in the present study to evaluate the protective effect of silver nanoparticles biosynthesized using Fig and Olive extracts against cutaneous lesions induced by Leishmania major infection through their anti-inflammatory, antioxidant activities, and faster clinical efficacy than standard pentavalent antimonial treatment.

scholarly journals Antioxidant, Anti-Inflammatory and Wound Healing of Biosynthetic Gold Nanoparticles Using Mangosteen (G. Mangostona)

2021 ◽  
Vol 8 (2) ◽  
pp. 59-74
Author(s):  
Abdulkadir Mohammed Noori Jassim ◽  
Gufran Mohammed Shafy ◽  
Mustafa Taha Mohammed ◽  
Safana Ahmed Farhan ◽  
Omar Mohammed Noori
Keyword(s):  
Gold Nanoparticles ◽  
Wound Healing ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Spectroscopic Method ◽  
Radical Scavenging ◽  
New Drugs ◽  
Garcinia Mangostana ◽  
Anti Inflammatory ◽  
Peel Extract

In current research, the synthesis of gold nanoparticles was achieved via reducing of gold ions in aqueous solution with Garcinia mangostana (G. mangostana) peel extract. The optimum concentration of gold (Au) solution, concentration ratio of Au solution and extract, temperature, time and pH, the synthesized AuNPs (G. mangostana-gold nanoparticles) were studied by using UV-Vis, FT-IR, AAS, AFM, SEM and Zitasizer. The absorbance peak is noticed between 535-550 nm via UV-Vis spectroscopic method. The SEM, AFM analysis were proofed the particle as spherical in structure and their size between 15-100nm. Therefore, mechanism of AuNPs synthesis had been suggested. Also, the antibacterial activity was examined using different bacteria as well as free radical scavenging activity was tested using 1, 1-Diphenyl-2-picrylhydrazyl (DPPH). The AuNPs produced through biosynthesized method indicated a much elevated antioxidant activity as compared to peel extract of G. mangostana. Toxicity of the NPs and extract were tested via giving orally dose 50 mg/b.w. to mice. Diagnosis of the data (pathological changes) indicated that the AuNPs was non-toxic. The G. mangostana peel extract and AuNPs synthesized by this extract were converted to a cream and used as a wound healing cream. As a results, the AuNPs exhibited important role in wound healing progression compared to control, which may be attributed to their anti-inflammatory, antibacterial and antioxidant activities. Therefore, this research confirms its important use of AuNPs and can be utilized as promising agents for in the development of new drugs.

scholarly journals Environmentally benevolent synthesis and characterization of silver nanoparticles using Olea ferruginea Royle for antibacterial and antioxidant activities

2020 ◽  
Vol 9 (1) ◽  
pp. 451-461 ◽  
Author(s):  
Abid Hussain ◽  
Ansar Mehmood ◽  
Ghulam Murtaza ◽  
Khawaja Shafique Ahmad ◽  
Aneela Ulfat ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Cost Effective ◽  
Positive Control ◽  
Ag Nps ◽  
Stabilizing Agent ◽  
Mean Size ◽  
Spherical Crystals

AbstractIn this study, we reported an easy, rapid, cost-effective and environmentally benign method for the fabrication of silver nanoparticles (Ag-NPs) using Olea ferruginea as reducing, capping and stabilizing agent. For this, an aqueous extract of leaf and bark of O. ferruginea was treated with 1 mM AgNO3, which reduces Ag ions to Ag-NPs by establishing reddish brown color. The synthesized Ag-NPs were spherical crystals, with a mean size of 23 and 17 nm for leaf- and bark-mediated Ag-NPs, respectively. Fourier transform infrared spectroscopy affirmed the role of leaf and bark extracts of O. ferruginea as reducing, capping and stabilizing agent. These biosynthesized Ag-NPs showed profound antibacterial activity against Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Streptococcus pneumonia and Staphylococcus aureus) bacteria. The highest antibacterial activity was shown by bark Ag-NPs against S. aureus (14.00 mm), while leaf Ag-NPs showed higher activity against S. pneumonia (13.00 mm). Additionally, they produced effective antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) as compared to plant extracts and positive control. It was observed that the bark-mediated Ag-NPs had higher percentage (90%) of scavenging potential than the leaf-mediated Ag-NPs (78%). The significance of the current study is the synthesis of eco-friendly, easy and cost-effective Ag-NPs as biomedical products.

scholarly journals Biosynthesis of silver nanoparticles using citrus sinensis peel extract and their application as antibacterial agent

2020 ◽  
Vol 11 (3) ◽  
pp. 4726-4732
Author(s):  
Sharanappa A ◽  
Anil R. Shet ◽  
Laxmikant R. Patil ◽  
Veeresh S. Hombalimath ◽  
Santosh Kadapure
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Citrus Sinensis ◽  
Antibacterial Agent ◽  
Chemical Method ◽  
Nitrate Concentration ◽  
Ag Nps ◽  
Extract Concentration ◽  
Silver Nitrate Concentration ◽  
Peel Extract

Silver nanoparticles (Ag-NPs) have attracted huge importance due to their distinctive chemical, biological and physical properties. Silver nanoparticles are widely synthesized by the chemical method, which involves the use of toxic chemicals which affects its applications. The bio-reduction method, in comparison with chemical method is more economic and eco-friendly. In the present work, the bio-based production of Ag-NPs was done by using peel extract of orange (citrus sinensis), which played a role of reducing and stabilizing agent. The biosynthesis of silver nanoparticles was optimized by one factor at a time (OFAT) with respect to peel extract concentration, silver nitrate concentration and reaction temperature. The green synthesized silver nanoparticles were characterized by UV-visible spectroscopy, Fourier transforms infrared (FT-IR) spectroscopy, Scanning electron microscopy (SEM) and X-ray diffraction (XRD). Disk diffusion method was used for the study of antibacterial activity of the bio-synthesized silver nanoparticles against the bacteria Escherichia coli and Staphylococcus aureus. The results showed that at a peel extract concentration of 6%, the temperature of 60oC and silver nitrate concentration of 0.1M, the synthesis of Ag-NPs was effective. The orange peel synthesized Ag-NPs showed effective antibacterial activity against both bacteria. However better activity was observed against bacterium Staphylococcus aureus. The results confirmed the synthesis of Ag-NPs using peel extract of citrus sinensis and its role as antibacterial agent.

scholarly journals Green synthesis of silver nanoparticles using Omani pomegranate peel extract and two polyphenolic natural products: characterization and comparison of their antioxidant, antibacterial, and cytotoxic activities

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Pasent Gharib Saad ◽  
Rohan Daniel Castelino ◽  
Vimal Ravi ◽  
Issa Sulaiman Al-Amri ◽  
Shah Alam Khan
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Average Particle Size ◽  
Cytotoxic Activities ◽  
Average Particle ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract Background Green synthesis of silver nanoparticles (AgNPs) has gained popularity due to the economical and eco-friendly approach associated with it. The aim of the study was to biosynthesize silver nanoparticles using pomegranate peel extract (PPE), quercetin (Q), and gallic acid (GA) and to evaluate their antioxidant, antimicrobial, and cytotoxic activities. Results A sharp color change from yellow to brown, appearance of a peak in a UV spectrum around 413–425 nm, and four intense peaks in XRD spectra matching with the reference silver crystal confirmed the formation of AgNPs. SEM and TEM analysis revealed particles to be mostly spherical with few aggregates. Average particle size distribution and zeta potential was found to be 43.6 nm and −18.01mV, 31.6 nm and −21.5 mV, and 21.7 nm and −27.9 mV for Q-AgNPs, P-AgNPs, and GA-AgNPs, respectively. P-AgNPs showed excellent in vitro antioxidant activity (84.85–89.20%) and cytotoxic activity (100% mortality). P-AgNPs and GA-AgNPs exhibited good antimicrobial activity against the four bacterial strains comparable to the reference antibiotics but Q-AgNPs failed to inhibit the growth of P. vulgaris. Conclusions Omani pomegranate peel extract seems to be a potential and alternative source for the simple, one pot, eco-friendly green synthesis of extracellular stable AgNPs. The synthesized nanoparticles have the potential to be developed as possible antimicrobial agents and antioxidants. Graphical abstract

scholarly journals Dyeing of cotton fabric materials with biogenic gold nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Sivakavinesan ◽  
M. Vanaja ◽  
G. Annadurai
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Color Change ◽  
Nanoparticle Synthesis ◽  
Ion Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Fruit Peel ◽  
Garcinia Mangostana ◽  
Powder Concentration ◽  
Peel Extract

AbstractThe present work aimed at synthesizing gold nanoparticles in a biological method employing fruit peel waste dumped in the environment. The peels of Garcinia mangostana (Mangostan), were collected from the nearby tourist spot during the season. The collected fruit peels were washed, dried, powder and extracted by using boiling water and acetone. The precipitated extract was dried and powdered for further use. The dried and powdered peel extract was added to the gold solution and boiled to 80 °C and the color change is observed. The color change indicates the completion of the synthesis of gold nanoparticles. The effect of pH, gold ion concentration, peel extract powder concentration, and the temperature was tested by varying the parameters. The biosynthesized nanoparticles were characterized using the UV–Vis spectrophotometer to identify the surface plasmon resonance peaks corresponding to gold nanoparticles. The bio-moieties responsible for the synthesis of gold nanoparticles were identified using the Fourier Transform Infra-Red Spectroscopy. The crystalline nature was detected by using an X-Ray Diffractometer. Atomic Force Microscope viewed the 3D surface image of the gold nanoparticle. The shape and morphology of the nanoparticle were identified by using a Field Emission Scanning Electron Microscope. The active compounds for gold nanoparticle synthesis were identified using Gas Chromatography-Mass Spectrometry. The gold nanoparticle was synthesized in various colors and used for dyeing cotton fabrics. The dyed cotton materials were exposed to various stress conditions to determine the color fastening.

scholarly journals Synthesis of silver nanoparticles using Plantago lanceolata extract and assessing their antibacterial and antioxidant activities

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Zahir Shah ◽  
Zheng-Hui Guan ◽  
Ala Ud Din ◽  
Amjad Ali ◽  
Ata Ur Rehman ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Plantago Lanceolata ◽  
Lethal Dose ◽  
Antibacterial Activities ◽  
Future Research ◽  
Physical Interaction ◽  
Ag Nps ◽  
Bioactive Natural Products

AbstractSilver nanoparticles (Ag. NPs) have shown a biological activity range, synthesized under different environment-friendly approaches. Ag. NPs were synthesized using aqueous crude extract (ACE) isolated from Plantago lanceolata. The ACE and Ag. NPs were characterized and assessed their biological and antioxidant activities. The existence of nanoparticles (NPs) was confirmed by color shift, atomic force microscopy (AFM), and UV–Vis’s spectroscopy. The FT-IR analysis indicated the association of biomolecules (phenolic acid and flavonoids) to reduce silver (Ag+) ions. The SEM study demonstrated a sphere-shaped and mean size in the range of 30 ± 4 nm. The EDX spectrum revealed that the Ag. NPs were composed of 54.87% Ag with 20 nm size as identified by SEM and TEM. AFM has ended up being exceptionally useful in deciding morphological elements and the distance across of Ag. NPs in the scope of 23–30 nm. The TEM image showed aggregations of NPs and physical interaction. Ag. NPs formation also confirmed by XPS, DRS and BET studies. Ag. NPs showed efficient activity as compared to ACE, and finally, the bacterial growth was impaired by biogenic NPs. The lethal dose (LD50) of Ag. NPs against Agrobacterium tumefaciens, Proteus vulgaris, Staphylococcus aureus, and Escherichia coli were 45.66%, 139.71%, 332.87%, and 45.54%, with IC50 (08.02 ± 0.68), (55.78 ± 1.01), (12.34 ± 1.35) and (11.68 ± 1.42) respectively, suppressing the growth as compared to ACE. The antioxidant capacity, i.e., 2,2-diphenyl-1-picrylhydrazyl (DPPH) of Ag. NPs were assayed. ACE and Ag. NPs achieved a peak antioxidant capacity of 62.43 ± 2.4 and 16.85 ± 0.4 μg mL−1, compared to standard (69.60 ± 1.1 at 100 μg mL−1) with IC50 (369.5 ± 13.42 and 159.5 ± 10.52 respectively). Finally, the Ag. NPs synthesized by P. lanceolata extract have an excellent source of bioactive natural products (NP). Outstanding antioxidant, antibacterial activities have been shown by NPs and can be used in various biological techniques in future research.

scholarly journals Comparison of the antibacterial activity against Escherichia coli of silver nanoparticle produced by chemical synthesis with biosynthesis

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Nguyen Phuc Quan 1,2 ◽  
Tran Quoc Vinh 1 ◽  
Kieu Thi My Yen 1 ◽  
Le Vu Khanh Trang 2 ◽  
Nguyen Minh Ly 2 ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Color Change ◽  
Chemical Reduction ◽  
Spherical Shape ◽  
Average Diameter ◽  
Ag Nps ◽  
E Coli ◽  
Vis Spectroscopy

The synthesis of silver nanoparticles (Ag NPs) has been carried out using different methods, mainly by biological and chemical methods; however, comparing antibacterial activity of Ag NPs synthesized by these methods has not been conducted before. In this study, silver nanoparticles (Ag NPs) were synthesized by methods using reducing agent NaBH4/carboxymethyl cellulose (CMC) and fungal strain Trichoderma asperellum (T.asperellum). The formation of silver nanoparticles was observed visually by color change and identified by Ultraviolet-visible (UV – vis) spectroscopy. The transmission electron microscopy (TEM) image illustrated almost nanoparticles with spherical shape and average diameter of 4.1 ± 0.2 nm and 2.1 ± 0.2 nm of samples produced from chemical reduction and biosynthesis respectively. Both samples after 180 days storing have been separated lightly, but the agglomeration and absorbance peak shifting were not observed which proved the high stability of synthesized Ag NPs. Antimicrobial activity against human bacterial pathogen Escherichia coli (E. coli) showed that the inhibition zone produced by “biosynthesis” and “chemical reduction” Ag NPs were 3.17 cm and 2.42 cm respectively. With nanoparticles size smaller than 2 mm, antibacterial activity of “biosynthesis” Ag NPs against E. coli was 31 % higher than “chemical reduction” Ag NPs, although the concentration of Ag NPs produced by biosynthesis was about 10-fold less.

scholarly journals In-vitro evaluation of antioxidant and antiradical potential of successive extracts, semi-purified fractions and biosynthesized silver nanoparticles of Rumex vesicarius

2021 ◽  
Vol 49 (1) ◽  
pp. 12293
Author(s):  
Kareem M. YOUNES ◽  
Ramy M. ROMEILAH ◽  
Hossam S. EL-BELTAGI ◽  
Hani EL MOLL ◽  
Saravanan RAJENDRASOZHAN ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Absolute Methanol ◽  
Ag Nps ◽  
Synthetic Standard

The aim of the present study was to assess in vitro the antiradical and antioxidant activities of successive extracts and semi-purified fractions from Rumex vesicarius L. In the present work, three extracts (n-Hexane, ethyl acetate and methanol) and 22 column fractions of methanolic extract (as promising extract) were evaluated against 2,2-diphenyl- 1-picrylhydrazyl (DPPH•) and 2,2-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) radical scavenging methods as antiradical and antioxidant activities compared with Butylated hydroxytoluene (BHT) as synthetic standard and silver nanoparticles of methanolic extract (Ag-NPs-Me), in addition to analysis of   chemical constituents of extract and fraction using Gas chromatography–mass spectrometry (GC-MS). The obtained results revealed that, both methods go parallel showing that the concentration of extract and incubation time are dependent and proportional with phenolic compounds concentration.  Absolute methanol extract recorded the highest antioxidant activity when compared with the other crude extracts with 79.3 and 78.8% against DPPH and ABTS respectively when compared with BHT as synthetic standard (89.4 and 89.9%) against DPPH and ABTS respectively. Calculation of the antiradical activity units showed the highest values of methanolic extract and its promising fraction (No. 12) after 300 seconds (5 minutes) comparing with antioxidant activity (30 min). Also, the antioxidant activity increased with synthetic Ag-NPs-Me when compared with methanolic extract by (IC50= 53.9 and 74.6 µg/ml respectively).  Thus, the GC-MS analysis of successive extracts of R. vesicarius L showed a highly complex profile, containing approximately 24 different components. One pure compound was identified from fraction No. 12. The identified compound was l-(+)- ascorbic acid 2, 6-dihexadecanoate. The data also revealed presence of closely similar antioxidant activities in methanolic extract or its pure compounds with BHT when mixed at different proportions. From the obtained results it could be concluded that R. vesicarius methanolic extracts and fractions can be extensively used in the production of potential antioxidant, antiradical and AgNPs-Me for biomedical application on the consumer’s health.

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

2019 ◽  
Vol 12 (5) ◽  
pp. 4644-4647
Author(s):  
K.K. Gupta ◽  
Neha Kumari ◽  
Neha Sinha ◽  
Akruti Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Bacterial Species ◽  
Antimicrobial Property ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Light Yellow ◽  
Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

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