scholarly journals The Effect of Sea Buckthorn (Hippophae rhamnoides L.) Seed Oil on UV-Induced Changes in Lipid Metabolism of Human Skin Cells

Antioxidants ◽  
2018 ◽  
Vol 7 (9) ◽  
pp. 110 ◽  
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Iwona Jarocka-Karpowicz ◽  
Marta Muszyńska ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Mass Spectrometry ◽  
Lipid Metabolism ◽  
Seed Oil ◽  
Cannabinoid Receptor ◽  
Redox Balance ◽  
Sea Buckthorn ◽  
Ros Generation ◽  
Enzymatic Antioxidants ◽  
Skin Cells ◽  
Sea Buckthorn Oil

Lipids and proteins of skin cells are the most exposed to harmful ultraviolet (UV) radiation contained in sunlight. There is a growing need for natural compounds that will protect these sensitive molecules from damage, without harmful side effects. The aim of this study was to investigate the effect of sea buckthorn seed oil on the redox balance and lipid metabolism in UV irradiated cells formed different skin layers to examine whether it had a protective effect. Human keratinocytes and fibroblasts were subjected to UVA (ultraviolet type A; 30 J/cm2 and 20 J/cm2) or UVB (ultraviolet type B; 60 mJ/cm2 and 200 mJ/cm2, respectively) radiation and treated with sea buckthorn seed oil (500 ng/mL), and the redox activity was estimated by reactive oxygen species (ROS) generation and enzymatic/non-enzymatic antioxidants activity/level (using electron spin resonance (ESR), high-performance liquid chromatography (HPLC), and spectrophotometry). Lipid metabolism was measured by the level of fatty acids, lipid peroxidation products, endocannabinoids and phospholipase A2 activity (GC/MS (gas chromatography/mass spectrometry), LC/MS (liquid chromatography/mass spectrometry), and spectrophotometry). Also, transcription factor Nrf2 (nuclear erythroid 2-related factor) and its activators/inhibitors, peroxisome proliferator-activated receptors (PPAR) and cannabinoid receptor levels were measured (Western blot). Sea buckthorn oil partially prevents UV-induced ROS generation and enhances the level of non-enzymatic antioxidants such as glutathione (GSH), thioredoxin (Trx) and vitamins E and A. Moreover, it stimulates the activity of Nrf2 leading to enhanced antioxidant enzyme activity. As a result, decreases in lipid peroxidation products (4-hydroxynonenal, 8-isoprostaglandin) and increases in the endocannabinoid receptor levels were observed. Moreover, sea buckthorn oil treatment enhanced the level of phospholipid and free fatty acids, while simultaneously decreasing the cannabinoid receptor expression in UV irradiated keratinocytes and fibroblasts. The main differences in sea buckthorn oil on various skin cell types was observed in the case of PPARs—in keratinocytes following UV radiation PPAR expression was decreased by sea buckthorn oil treatment, while in fibroblasts the reverse effect was observed, indicating an anti-inflammatory effect. With these results, sea buckthorn seed oil exhibited prevention of UV-induced disturbances in redox balance as well as lipid metabolism in skin fibroblasts and keratinocytes, which indicates it is a promising natural compound in skin photo-protection.

scholarly journals Addition of Reduced Glutathione (GSH) to Freezing Medium Reduces Intracellular ROS Levels in Donkey Sperm

2021 ◽  
Vol 8 (12) ◽  
pp. 302
Author(s):  
Iván Yánez-Ortiz ◽  
Jaime Catalán ◽  
Ariadna Delgado-Bermúdez ◽  
Augusto Carluccio ◽  
Jordi Miró ◽  
...  
Keyword(s):  
Reduced Glutathione ◽  
Membrane Integrity ◽  
Redox Balance ◽  
Low Fertility ◽  
Ros Generation ◽  
Enzymatic Antioxidants ◽  
Freezing Medium ◽  
Exogenous Addition ◽  
Sperm Plasma Membrane ◽  
The Impact

In donkeys, the use of frozen-thawed sperm for artificial insemination (AI) leads to low fertility rates. Furthermore, donkey sperm produce a large amount of reactive oxygen species (ROS), and post-AI inflammation induces the formation of neutrophil extracellular traps (NETosis), which further generates many more ROS. These high ROS levels may induce lipid peroxidation in the sperm plasma membrane, thus affecting its integrity. Enzymatic and non-enzymatic antioxidants, mainly found in the seminal plasma (SP), are responsible for maintaining the redox balance. However, this fluid is removed prior to cryopreservation, thereby exposing sperm cells to further oxidative stress. The exogenous addition of antioxidants to the freezing medium can reduce the detrimental effects caused by ROS generation. Therefore, the aim of this study was to evaluate how the addition of different reduced glutathione (GSH) concentrations (control, 2 mM, 4 mM, 6 mM, 8 mM, and 10 mM) to fresh sperm affect their cryotolerance. Total and progressive motility, kinematic parameters and motile sperm subpopulations were significantly (p < 0.05) different from the control in treatments containing 8 mM and 10 mM GSH, but not at lower concentrations. Plasma and acrosome membrane integrity, mitochondrial membrane potential (MMP) and intracellular superoxide levels (O2−) were not affected (p > 0.05) by any GSH concentration. Interestingly, however, the addition of 8 mM or 10 mM GSH reduced (p < 0.05) the percentages of viable sperm with high overall ROS levels compared to the control. In conclusion, frozen-thawed donkey sperm are able to tolerate high GSH concentrations, which differs from what has been observed in other species. This antioxidant capacity suggests that ROS could be important during post-AI and that the impact of using exogenous antioxidants like GSH to improve the sperm resilience to freeze-thawing is limited in this species.

Potential health benefits of Sea buckthorn oil- A review

2017 ◽  
Vol 38 (03) ◽  
Author(s):  
Vinita . ◽  
Darshan Punia ◽  
Neeta Kumari
Keyword(s):  
Palmitoleic Acid ◽  
Seed Oil ◽  
Sea Buckthorn ◽  
Bioactive Components ◽  
Potential Health ◽  
Oral Fluids ◽  
Medicinal Value ◽  
Mucous Membranes ◽  
Sea Buckthorn Oil ◽  
High Level

Hippophae rhamnoides, also known as sea buckthorn is an ancient plant with modern virtues, due to its nutritional and medicinal value. Sea buckthorn berry is rich in oil. Seeds (seed oil) and fruit pulp (pulp oil) are used for oil extraction. The oil content of Sea buckthorn pulp and seeds are in range of 18.2-43.5% and 7.03 -12.86% respectively. While linoleic (18:2n-6) and á-linolenic (18:3n-3) acids are the major fatty acids in the seed oil, the high level of palmitoleic acid (16:1n-7, up to 50%) differentiates sea buckthorn pulp oil from most other oils of plant origin. Oil from sea buckthorn contains several bioactive components such as vitamin E, vitamin K, carotenoids and phytosterols. Sea buckthorn oil has shown great healing effect in treating damaged mucous membranes of the gastrointestinal tract, wound, burn, dry eye, dermatitis and sunburn. The oil from pulp and the seeds of sea buckthorn have been shown to possess anti-diabetic, anti-atherogenic, hypocholesteromic, hypotensive, anti-hypoxic, antioxidant and anti-inflammatory properties. Sea buckthorn oils are used as a source for ingredients in several commercially available cosmetic products and nutritional supplements like jelly, plant capsules, or oral fluids.

scholarly journals A Fatty Acid Fraction Purified From Sea Buckthorn Seed Oil Has Regenerative Properties on Normal Skin Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Maria Dudau ◽  
Elena Codrici ◽  
Isabela Tarcomnicu ◽  
Simona Mihai ◽  
Ionela Daniela Popescu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Seed Oil ◽  
Biological Effects ◽  
Normal Skin ◽  
Cell Types ◽  
Fatty Acid Fraction ◽  
Sea Buckthorn ◽  
Technological Advancement ◽  
Skin Cells

In recent years, natural product's research gained momentum, fueled by technological advancement and open availability of research data. To date, sea buckthorn (Hippophae rhamnoides L. [Elaeagnaceae]) plant parts, especially berries, are well characterized and repeatedly tested for antioxidant activity and regenerative properties, in various cell types and tissues. However, fatty acids (FA) have been less investigated in term of biological effects, although, they are important bioactive components of the sea buckthorn fruit and oil. The aim of our work was to determine whether sea buckthorn seed oil is a suitable source of FA with regenerative properties on normal skin cells. Using high-performance liquid chromatography (HPLC) and liquid chromatography – mass spectrometry (LC-MS), we purified and characterized four fractions enriched in saturated (palmitic) and non-saturated (linoleic, alfa-linolenic, oleic) FA, which were tested for cytotoxicity, cytokine and growth factor production, and regenerative effect on normal keratinocytes and skin fibroblasts. Evidence is presented that the palmitic acid enriched fraction was a suitable sea buckthorn seed oil derived product with cell proliferation properties on both skin cell types.

scholarly journals Sea-Buckthorn Seed Oil Induces Proliferation of both Normal and Dysplastic Keratinocytes in Basal Conditions and under UVA Irradiation

2021 ◽  
Vol 11 (4) ◽  
pp. 278
Author(s):  
Maria Dudau ◽  
Alexandra Catalina Vilceanu ◽  
Elena Codrici ◽  
Simona Mihai ◽  
Ionela Daniela Popescu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Seed Oil ◽  
Essential Fatty Acids ◽  
Human Keratinocytes ◽  
Sea Buckthorn ◽  
Herbal Remedies ◽  
Skin Cells ◽  
Real Time Analysis ◽  
Uva Irradiation ◽  
Long Chain

Past decades demonstrate an increasing interest in herbal remedies in the public eye, with as many as 80% of people worldwide using these remedies as healthcare products, including those for skin health. Sea buckthorn and its derived products (oil; alcoholic extracts), rich in flavonoids and essential fatty acids, are among these healthcare products. Specifically, sea buckthorn and its derivatives are reported to have antioxidant and antitumor activity in dysplastic skin cells. On the other hand, evidence suggests that the alteration of lipid metabolism is related to increased malignant behavior. Given the paradoxical involvement of lipids in health and disease, we investigated how sea-buckthorn seed oil, rich in long-chain fatty acids, modifies the proliferation of normal and dysplastic skin cells in basal conditions, as well as under ultraviolet A (UVA) radiation. Using real-time analysis of normal and dysplastic human keratinocytes, we showed that sea-buckthorn seed oil stimulated the proliferation of dysplastic cells, while it also impaired the ability of both normal and dysplastic cells to migrate over a denuded area. Furthermore, UVA exposure increased the expression of CD36/SR-B2, a long-chain fatty acid translocator that is related to the metastatic behavior of tumor cells.

scholarly journals Identification and Analytical Characterization of a Novel Synthetic Cannabinoid-Type Substance in Herbal Material in Europe

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 793
Author(s):  
Emmanouil D. Tsochatzis ◽  
Joao Alberto Lopes ◽  
Margaret V. Holland ◽  
Fabiano Reniero ◽  
Giovanni Palmieri ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cannabinoid Receptor ◽  
Analytical Data ◽  
Synthetic Cannabinoids ◽  
Synthetic Cannabinoid ◽  
Gas Chromatography Mass Spectrometry ◽  
New Psychoactive Substances ◽  
Synthetic Cannabinoid Receptor Agonists ◽  
Analytical Laboratories

The rapid diffusion of new psychoactive substances (NPS) presents unprecedented challenges to both customs authorities and analytical laboratories involved in their detection and characterization. In this study an analytical approach to the identification and structural elucidation of a novel synthetic cannabimimetic, quinolin-8-yl-3-[(4,4-difluoropiperidin-1-yl) sulfonyl]-4-methylbenzoate (2F-QMPSB), detected in seized herbal material, is detailed. An acid precursor 4-methyl-3-(4,4-difluoro-1-piperidinylsulfonyl) benzoic acid (2F-MPSBA), has also been identified in the same seized material. After extraction from the herbal material the synthetic cannabimimetic, also referred to as synthetic cannabinoid receptor agonists or “synthetic cannabinoids”, was characterized using gas chromatography-mass spectrometry (GC-MS), 1H, 13C, 19F and 15N nuclear magnetic resonance (NMR) and high-resolution tandem mass spectrometry (HR-MS/MS) combined with chromatographic separation. A cheminformatics platform was used to manage and interpret the analytical data from these techniques.

scholarly journals Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Oliver C. Watkins ◽  
Preben Selvam ◽  
Reshma Appukuttan Pillai ◽  
Victoria K. B. Cracknell-Hazra ◽  
Hannah E. J. Yong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Lipid Metabolism ◽  
Metabolic Pathways ◽  
Liquid Chromatography Mass Spectrometry ◽  
Fasting Glycemia ◽  
Glucose Treatment ◽  
Maternal Bmi

Abstract Background Fetal docosahexaenoic acid (DHA) supply relies on preferential transplacental transfer, which is regulated by placental DHA lipid metabolism. Maternal hyperglycemia and obesity associate with higher birthweight and fetal DHA insufficiency but the role of placental DHA metabolism is unclear. Methods Explants from 17 term placenta were incubated with 13C-labeled DHA for 48 h, at 5 or 10 mmol/L glucose treatment, and the production of 17 individual newly synthesized 13C-DHA labeled lipids quantified by liquid chromatography mass spectrometry. Results Maternal BMI positively associated with 13C-DHA-labeled diacylglycerols, triacylglycerols, lysophospholipids, phosphatidylcholine and phosphatidylethanolamine plasmalogens, while maternal fasting glycemia positively associated with five 13C-DHA triacylglycerols. In turn, 13C-DHA-labeled phospholipids and triacylglycerols positively associated with birthweight centile. In-vitro glucose treatment increased most 13C-DHA-lipids, but decreased 13C-DHA phosphatidylethanolamine plasmalogens. However, with increasing maternal BMI, the magnitude of the glucose treatment induced increase in 13C-DHA phosphatidylcholine and 13C-DHA lysophospholipids was curtailed, with further decline in 13C-DHA phosphatidylethanolamine plasmalogens. Conversely, with increasing birthweight centile glucose treatment induced increases in 13C-DHA triacylglycerols were exaggerated, while glucose treatment induced decreases in 13C-DHA phosphatidylethanolamine plasmalogens were diminished. Conclusions Maternal BMI and glycemia increased the production of different placental DHA lipids implying impact on different metabolic pathways. Glucose-induced elevation in placental DHA metabolism is moderated with higher maternal BMI. In turn, findings of associations between many DHA lipids with birthweight suggest that BMI and glycemia promote fetal growth partly through changes in placental DHA metabolism.

Aloe vera gel and sea-buckthorn oil protect human fibroblasts against silver nanoparticles cytotoxicity

2021 ◽  
Vol 350 ◽  
pp. S238-S239
Author(s):  
A.-M. Enciu ◽  
E. Codrici ◽  
D.I. Popescu ◽  
P. Preda ◽  
D. Stan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Human Fibroblasts ◽  
Aloe Vera ◽  
Sea Buckthorn ◽  
Aloe Vera Gel ◽  
Sea Buckthorn Oil

scholarly journals High performance liquid chromatography coupled with high resolution mass spectrometry-based characterization of lipidomic responses from rats with kidney injuries

RSC Advances ◽  
2018 ◽  
Vol 8 (36) ◽  
pp. 20250-20258
Author(s):  
Qun Liang ◽  
Han Liu ◽  
Xiuli Li ◽  
Yang Zhang
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lipid Metabolism ◽  
High Resolution ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Resolution Mass ◽  
Resolution Mass

In this study, we present targeted and non-targeted lipidomics strategies to discover the lipid metabolism variation in serum in rats with sepsis-induced kidney injuries.

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