scholarly journals Characterization of an Antioxidant and Antimicrobial Extract from Cool Climate, White Grape Marc

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 232 ◽  
Author(s):  
Kenneth J. Olejar ◽  
Arianna Ricci ◽  
Simon Swift ◽  
Zoran Zujovic ◽  
Keith C. Gordon ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Agricultural Waste ◽  
Antibacterial Properties ◽  
Total Phenolic ◽  
Gram Positive Bacteria ◽  
Winery Waste ◽  
And Staphylococcus Aureus ◽  
Spectral Assessment ◽  
Cool Climate

Valorization of agricultural waste has become increasingly important. Wastes generated by wineries are high in phenolic compounds with antioxidant and antibacterial properties, which contribute to phytotoxicity, making their immediate use for agricultural means limited. Utilizing a water-based extraction method, the phenolic compounds from winery waste were extracted and purified. The resulting extract was characterized for phenolic composition using high-pressure liquid chromatography-ultraviolet/visible and electrochemical detectors (HPLC-UV/Vis, ECD) for monomers, and spectral assessment of the tannins present using attenuated total reflectance- Fourier transform infrared (ATR-FTIR), FT-Raman, and solid-state nuclear magnetic resonance (SSNMR) spectroscopies. The extract’s antioxidant activity was assessed by the scavenging of the 2,2-diphenyl-1–picrylhydrazyl (DPPH) radical and Folin-Ciocalteu total phenolic assay, and was found to be as effective as a commercially obtained grape extract. The extract’s antimicrobial efficacy was tested for minimum bactericidal concentration using Candida albicans, Escherichia coli 25922, and Staphylococcus aureus 6538, which resulted in greater efficacy against gram-positive bacteria as shown over gram-negative bacteria, which can be linked to both monomeric and tannin polyphenols, which have multiple modes of bactericidal action.

scholarly journals Phenolic compounds, antioxidant and antimicrobial properties of the wild cherry (Prunus avium L.) stem

2017 ◽  
pp. 1-13 ◽  
Author(s):  
Zahida Ademovic ◽  
Snjezana Hodzic ◽  
Zarka Halilic-Zahirovic ◽  
Darja Husejnagic ◽  
Jasna Dzananovic ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Prunus Avium ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Aqueous Extracts ◽  
Wild Cherry ◽  
Gram Positive Bacteria ◽  
Prunus Avium L

The aim of this study was to determine the total phenolic content, evaluate antioxidant propertie and antimicrobial potential, and identify phenolic compounds in alcoholic and aqueous extracts of the wild cherry (Prunus avium L.) stems collected in Bosnia and Herzegovina. Alcoholic extracts had higher contents of phenolic and flavonoid components, as well as the antioxidant and ferric reducing antioxidant capacity in comparison to aqueous extracts. All extracts were characterized by HPLC analysis. Furthermore, for the first time, the antimicrobial properties of wild cherry stem extracts were evaluated. Quercetin and (+)-catechin were the main compounds identified in the alcoholic extract, followed by chlorogenic acid and rutin. Quercetin was also the major component detected in aqueous extracts. Besides, alcoholic extract showed better antibacterial properties against Staphylococcus aureus as a representative gram-positive bacteria than infusion, whereas none of the samples showed antibacterial activity against Escherichia coli and fungus Candida albicans.

scholarly journals Analysis of Antioxidant and Antiallergic Active Components Extracted From the Edible Insect Oxya yezoensis

2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110233
Author(s):  
Masahiro Saiki ◽  
Naomichi Takemoto ◽  
Maki Nagata ◽  
Masako Matsumoto ◽  
Yhiya Amen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Unsaturated Fatty Acids ◽  
Soluble Fraction ◽  
Total Phenolic ◽  
Key Factors ◽  
Active Components ◽  
Food And Agriculture ◽  
Food And Agriculture Organization

In recent years, entomophagy has attracted increased attention, as it was recommended as a potential source of food by the Food and Agriculture Organization of the United Nations. In Japan, Oxya yezoensisis one of the most widely eaten insect species, but studies of its functionality as a food are limited. In this study, we reported the optimal characterization of the total phenolic compounds in methanolic extract (OME) and different fractions of OME. Additionally, the antioxidant and antiallergic activities of the OME fractions were evaluated. The results showed that the ethyl acetate-soluble fraction of OME has potential antioxidant activity, whereas the n-hexane-soluble fraction showed the strongest inhibition of β-hexosaminidase, which is one of the key factors in allergic reactions. It was concluded that phenolic compounds might contribute to the antioxidant activity while unsaturated fatty acids contribute to the antiallergy activity.

scholarly journals Evaluation of Extracts Obtained from FruitWastes Using Different Methods

Ingeniería ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 77-92
Author(s):  
Ciliana Florez Montes ◽  
Andrés Felipe Rojas González ◽  
Sneyder Rodríguez Barona
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Grape Seeds ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Pharmaceutical Industries

Context: Currently, the increase in agroindustrial waste generation has encouraged the search for viable use alternatives. In this paper, four methods to obtain extracts from mango, soursop, and grape peels, as well as and grape seeds, are studied. Their efficiency is analyzed through extraction yields and antioxidant capacity characterization of the extracts. Method: The extraction was performed using solvent, Soxhlet, microwave-assisted, and ultrasound assisted extraction. The characterization of the extracts was made by total phenolic compounds and flavonoids quantification, as well as antioxidant capacity determination, using the DPPH, FRAP, and ORAC tests. Results: It was found that grape seed extracts obtained by different extraction methods, highlighting those obtained by microwave assisted extraction, present a high total content phenolic compounds (>321.381,41 ± 3.476,85 μg Gallic Acid/g) and flavonoids (>103.232,01 ± 4.638,19 μg Quercetin/g), in addition to high antioxidant activity, according to the results of the DPPH (<1,06 ± 0,01), FRAP (>152.280,08 ± 5.197,53 µg TROLOX/g), and ORAC (>124.566,81 ± 581,96 μg TROLOX/g) tests. Conclusions: The results presented in this study suggest that the extracts obtained from grape seeds, especially those obtained by means of microwave-assisted extraction, have a potential use in food and pharmaceutical industries, due to their high antioxidant capacity and their total phenolic compounds and flavonoids content.

scholarly journals NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

2020 ◽  
Vol 83 (1) ◽  
pp. 19-25
Author(s):  
Suntini Suntini ◽  
Anastasia Wheni Indrianingsih ◽  
Harjono Harjono
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Antibacterial Properties ◽  
Reduction Process ◽  
Diffusion Method ◽  
Natural Material ◽  
Gram Positive Bacteria ◽  
Antibacterial Assay ◽  
Natural Composite

Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

scholarly journals The Effect of Drying Temperature on the Phenolic Content and Functional Behavior of Flours Obtained from Lemon Wastes

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 474 ◽  
Author(s):  
Jesús Patrón-Vázquez ◽  
Lizzie Baas-Dzul ◽  
Nelly Medina-Torres ◽  
Teresa Ayora-Talavera ◽  
Ángeles Sánchez-Contreras ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Content ◽  
Value Added ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Esi Ms ◽  
Functional Behavior ◽  
Industrial Sectors ◽  
A Value ◽  
And Staphylococcus Aureus

Lemon processing generates thousands of tons of residues that can be preserved as flours by thermal treatment to obtain phenolic compounds with beneficial bioactivities. In this study, the effect of different drying temperatures (40, 50, 60, 70, 80, 90, 100 and 110 °C) on the Total Phenolic Content (TPC), antioxidant and antimicrobial activities of phenolic compounds present in Citrus. lemon (L.) Burn f waste was determined. Identification and quantification of phenolic compounds were also performed by UPLC-PDA and UPLC-ESI-MS analysis. Eriocitrin (19.79–27.29 mg g−1 DW) and hesperidin (7.63–9.10 mg g−1 DW) were detected as the major phenolic compounds in the flours by UPLC-PDA and confirmed by UPLC-ESI-MS. Antimicrobial activity determined by Minimum Inhibitory Concentration (MIC) against Salmonella typhimurium, Escherichia coli and Staphylococcus aureus was observed. Accordingly, a stable functional flour as a source of bioactive phenolic compounds obtained from lemon residues at 50 °C may be produced as a value-added product useful in various industrial sectors.

scholarly journals A Characterization of Content, Composition and Scavenging Capacity of Phenolic Compounds in Parsnip Roots of Various Weight

2014 ◽  
Vol 9 (6) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Nada Č. Nikolić ◽  
Miodrag M. Lazić ◽  
Ivana T. Karabegović ◽  
Gordana S. Stojanović ◽  
Zoran B. Todorović
Keyword(s):  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Root Weight ◽  
Dry Extract ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Dpph Scavenging ◽  
Root Size

The contents, composition and radical scavenging capacity of phenolic compounds from parsnips of various root weights grown in Serbia were examined. The content of phenolic compounds depended on root size, and the highest content was in the group where the samples with the lowest root size were grouped. The content of phenolic compounds varied from 109.7 to 125.3 μmol of chlorogenic acid per g dry extract, i.e.5470.8 to 6245.1 mg per kg fresh weight. Among the identified compounds were quercetin, kaempferol, apigenin and genkwanin glucosides and hydroxycinnamic and caffeoylshikimic acids. The highest antioxidant capacity was found for the group with the lowest root weight; the IC50 values ranged from 1.59 to 2.49 mg mL–1. There was positive correlation between the total phenolic content and scavenging capacity, and the kaempferol glucosides content could be an indicator of DPPH scavenging capacity of parsnip roots.

scholarly journals Physico-chemical characterization of olive-oil mill wastewaters of Ben Karrich area (Tetouan province, North of Morocco) and optimization study of their treatment using activated carbon

2018 ◽  
Vol 7 (4) ◽  
pp. 253-258 ◽  
Author(s):  
Dorsaf Bouharat ◽  
Farida EL Yousfi ◽  
Anas Ellaghdach ◽  
Badr Dine Souhail ◽  
Nabila Slimani Alaoui
Keyword(s):  
Activated Carbon ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Oxygen Demand ◽  
Chemical Characterization ◽  
Operating Conditions ◽  
Organic Load ◽  
Total Phenolic ◽  
Total Phenolic Compounds

A physio-chemical characterization of olive oil mill wastewater (OMW) obtained from a continuous two-phases olive oil extraction process is presented. High organic load of OMW from Ben Karrich region has been registered. The values of chemical oxygen demand (COD) and total phenolic compounds were 84.5 g of O2/L and 3.79 g/L, respectively. In this sense, a treatment using activated carbon as adsorbent was proposed. Results have shown high removal percentages in total phenolic compounds (98%) and COD (90%). The optimal operating conditions were 4 g of activated carbon and adsorbing time equal to 5 minutes.

scholarly journals Evaluation of Direct Ultrasound-Assisted Extraction of Phenolic Compounds from Potato Peels

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1665
Author(s):  
Shusheng Wang ◽  
Amy Hui-Mei Lin ◽  
Qingyou Han ◽  
Qin Xu
Keyword(s):  
Phenolic Compounds ◽  
Agricultural Waste ◽  
The United States ◽  
Total Phenolic ◽  
Systematic Research ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Potato Peels ◽  
Ultrasound Assisted ◽  
The Impact

Potato peels (PPs) are generally considered as agriculture waste. The United States alone generates over one million tons of PPs a year. However, PPs contain valuable phenolic compounds with antioxidant activities. In this study, we evaluated the efficiency of ultrasound-assisted extraction techniques in recovering antioxidants from PPs. These techniques included a direct ultrasound-assisted extraction (DUAE), an indirect ultrasound-assisted extraction (IUAE), and a conventional shaking extraction (CSE). Results of this study showed that DUAE was more effective in extracting phenolic compounds than IUAE and CSE. We also evaluated the factors affecting the yield of total phenolic compounds (TPC) in DUAE, including the temperature, time, acoustic power, ratio of solvent to solids, and size of PPs particles. TPC yield of DUAE was higher, and the extraction rate was faster than IUAE and CSE. Furthermore, TPC yield was strongly correlated to the temperature of the mixture of PPs suspension. SEM images revealed that the irradiation of ultrasound energy from DUAE caused micro-fractures and the opening of PPs cells. The extract obtained from DUAE was found to have antioxidant activity comparable to commercial synthetic antioxidants. Results of this preliminary study suggest that DUAE has the potential to transform PPs from agricultural waste to a valuable ingredient. A future systematic research study is proposed to advance the knowledge of the impact of processing parameters in the kinetics of phenolic compounds extraction from potato peels using various extraction methods.

scholarly journals Fabrication and characterization of electrospun poly(e-caprolactone) fibrous membrane with antibacterial functionality

2017 ◽  
Vol 4 (2) ◽  
pp. 160911 ◽  
Author(s):  
Idris Cerkez ◽  
Ayse Sezer ◽  
Sukhwinder K. Bhullar
Keyword(s):  
Research Study ◽  
Silver Chloride ◽  
Antibacterial Properties ◽  
Mechanical Analysis ◽  
Fibrous Membrane ◽  
Gram Positive Bacteria ◽  
Lower Strain ◽  
Reinforcing Agent ◽  
Fabrication And Characterization

This research study is mainly targeted on fabrication and characterization of antibacterial poly(e-caprolactone) (PCL) based fibrous membrane containing silver chloride particles. Micro/nano fibres were produced by electrospinning and characterized with TGA, DSC, SEM and mechanical analysis. It was found that addition of silver particles slightly reduced onset of thermal degradation and increased crystallization temperature of neat PCL. Silver-loaded samples exhibited higher tensile stress and lower strain revealing that the particles behaved as reinforcing agent. Moreover, addition of silver chloride resulted in beaded surface texture and formation of finer fibres as opposed to the neat. Antibacterial properties were tested against Gram-negative and Gram-positive bacteria and remarkable biocidal functionalities were obtained with about six logs reduction of Staphylococcus aureus and Escherichia coli O157:H7.

scholarly journals Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1794 ◽  
Author(s):  
Wojciech Makowski ◽  
Krzysztof Michał Tokarz ◽  
Barbara Tokarz ◽  
Rafał Banasiuk ◽  
Karolina Witek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Total Phenolic ◽  
Tissue Cultures ◽  
Venus Flytrap ◽  
Dionaea Muscipula ◽  
Plant Culture

The carnivorous plant Dionaea muscipula J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of D. muscipula is very limited. Here, we describe an improved method to increase the quantity and quality of phenolic compounds produced in D. muscipula. This is achieved by combining biotic elicitation (using Cronobacter sakazakii bacteria lysate) of D. muscipula cultured with rotary shaking (hydromechanical stress), which we describe here for the first time. The antibacterial activity and the antioxidant properties of the obtained compounds were studied on two antibiotic-resistant human pathogenic bacteria. The proposed plant culture conditions resulted in an increase in fresh weight, as well as a higher total phenolic content, in comparison to traditional tissue cultures on agar-solidified medium. With the use of high-performance liquid chromatography, we demonstrated that the described elicitation strategy leads to an increased synthesis of myricetin, caffeic acid, ellagic acid and plumbagin in D. muscipula tissue. We also found that a higher level of antioxidant activity, exhibited by the plant extract, corresponded with its higher phenylpropanoid content. The bactericidal activity of the extract against Staphylococcus aureus was dependent on the duration of plant culture under described elicitation conditions, whereas neither elicitation condition (duration or elicitor concentration) seemed relevant for the bactericidal activity of the extract towards Escherichia coli. This suggest that Gram-negative bacteria are less sensitive to compounds derived from Venus flytrap tissue.

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