scholarly journals Changes in the Activity and Concentration of Superoxide Dismutase Isoenzymes (Cu/Zn SOD, MnSOD) in the Blood of Healthy Subjects and Patients with Acute Pancreatitis

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 948
Author(s):  
Milena Ściskalska ◽  
Monika Ołdakowska ◽  
Grzegorz Marek ◽  
Halina Milnerowicz
Keyword(s):  
Oxidative Stress ◽  
Acute Pancreatitis ◽  
Superoxide Dismutase ◽  
Healthy Subjects ◽  
Activity Concentration ◽  
Total Activity ◽  
Sod1 Gene ◽  
Markers Of Inflammation ◽  
Total Antioxidant ◽  
The Impact

This study was aimed at evaluating the changes in the concentration and activity of all superoxide dismutase isoenzymes (SOD1, SOD2, SOD3) in the blood of patients with acute pancreatitis (AP) and healthy subjects, taking into account the extracellular (plasma) and intracellular (erythrocyte lysate) compartment. The relationships between the activity/concentration of SODs, metal concentration and the markers of inflammation were evaluated. To assess the pro/antioxidative imbalance, the malonyldialdehyde (MDA) concentration and the value of total antioxidant capacity (TAC) were measured. The impact of single-nucleotide polymorphism (SNP) in the SOD1 gene (rs2070424) on the activity/concentration of SOD1 as the main isoenzyme of the SOD family was also analyzed in this study. The SOD2 activity in erythrocytes was increased compared to plasma: 10-fold in the AP patient group and 5-fold in healthy subjects. The plasma of AP patients showed an increased SOD1 concentration and decreased SOD2 and SOD3 concentrations compared to healthy subjects. The Cu/Zn SOD (SOD1 + SOD3) concentration in plasma of AP patients was elevated compared to healthy subjects, but changes in plasma Cu/Zn SOD (SOD1 + SOD3) activity in the examined groups were not observed. An influence of SNP rs2070424 in the SOD1 gene on the total activity of SOD in AP patients (with AG genotype), accompanied by an increased IL-6 concentration, was observed. In oxidative stress conditions induced by inflammation, the participation of individual forms of plasma SOD isoenzymes in total antioxidative activity of SOD changed. A significant increase in the intracellular SOD1 concentration in plasma of AP patients proves the important role of this isoenzyme in the neutralization of oxidative stress induced by impaired Cu and Zn homeostasis. The presence of increased concentration of SOD2 in erythrocytes of healthy subjects and AP patients confirms the important function of this isoenzyme in the antioxidative defense.

scholarly journals Importance of Genetic Polymorphisms in MT1 and MT2 Genes in Metals Homeostasis and Their Relationship with the Risk of Acute Pancreatitis Occurrence in Smokers—Preliminary Findings

2021 ◽  
Vol 22 (11) ◽  
pp. 5725
Author(s):  
Milena Ściskalska ◽  
Monika Ołdakowska ◽  
Halina Milnerowicz
Keyword(s):  
Acute Pancreatitis ◽  
Healthy Subjects ◽  
High Sensitivity ◽  
Nucleotide Polymorphisms ◽  
Reactive Protein ◽  
Markers Of Inflammation ◽  
Total Antioxidant ◽  
Hs Crp ◽  
Related Proteins ◽  
The Impact

This study was aimed at evaluating the changes in metallothionein (MT) concentration in the blood of patients with acute pancreatitis (AP) and healthy subjects, taking into account the extracellular (plasma) and intracellular (erythrocyte lysate) compartments. The impact of single-nucleotide polymorphisms (SNPs) in the MT1A (rs11640851), MT1B (rs964372) and MT2A (rs10636) genes on MT concentration and their association with the concentration of metals (Cu, Zn, Cd) and ceruloplasmin as Cu-related proteins were analyzed. The concentration of a high-sensitivity C-reactive protein (hs-CRP) and IL-6 as markers of inflammation, and malonyldialdehyde (MDA), superoxide dismutase (SODs) activity and the value of total antioxidant capacity (TAC) as parameters describing the pro/antioxidative balance were also assessed. In the AP patient groups, an increased MT concentration in erythrocyte lysate compared to healthy subjects was shown, especially in individuals with the GG genotype for rs964372 in the MT1B gene. A Zn concentration was especially decreased in the blood of smoking AP patients with the AA genotype for SNP rs11640851 in the MT1A gene and the GC genotype for SNP rs10636 in MT2A, compared to non-smokers with AP, which was accompanied by an increase in the value of the Cu/Zn ratio. The exposure to tobacco smoke xenobiotics increased the risk of AP occurrence in subjects with the CC genotype for SNP rs11640851 in the MT1A gene by more than fourfold. The investigated polymorphisms, rs11640851 in the MT1A gene, rs964372 in the MT1B gene and rs10636 in the MT2A gene, seem to be an important factor in maintaining homeostasis in an organism under oxidative stress conditions.

scholarly journals Decreased Lipid Profile and Oxidative Stress in Healthy Subjects Who Underwent Whole-Body Cryotherapy in Closed Cryochamber with Subsequent Kinesiotherapy

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Agata Stanek ◽  
Ewa Romuk ◽  
Tomasz Wielkoszyński ◽  
Stanisław Bartuś ◽  
Grzegorz Cieślar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
Healthy Subjects ◽  
Whole Body ◽  
Stress Index ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Whole Body Cryotherapy ◽  
The Impact ◽  
And Oxidative Stress

Objective. The aim of the study was to estimate the impact of whole-body cryotherapy (WBC) and subsequent kinesiotherapy on oxidative stress and lipid profile when performed in a closed cryochamber on healthy subjects. Material and Methods. The effect of ten WBC procedures lasting 3 minutes a day followed by a 60-minute session kinesiotherapy on oxidative stress and lipid profile in healthy subjects (WBC group, n=16) was investigated. The WBC group was compared to the kinesiotherapy only (KT; n=16) group. The routine parameters of oxidative stress (antioxidant enzymatic and nonenzymatic antioxidant status, lipid peroxidation products, total oxidative status (TOS), and oxidative stress index (OSI)) and lipid profile were estimated one day before the beginning and one day after the completion of the research program. Results. After treatment, in the WBC group, a significant decrease of oxidative stress markers (TOS and OSI) and a significant increase of total antioxidant capacity were observed. The activity of plasma SOD-Mn and erythrocyte total SOD increased significantly in the WBC group. In the KT group, the erythrocyte activity of total SOD, CAT, and GR decreased significantly after the treatment. The levels of T-Chol and LDL-Chol decreased significantly after treatment in both groups, but the observed decrease of these lipid parameters in the WBC group was higher in comparison to the KT group. The level of TG decreased significantly after treatment in the WBC group only. Conclusion. WBC performed in a closed cryochamber followed by kinesiotherapy improves lipid profile and decreases oxidative stress in healthy subjects.

scholarly journals The Association of Antioxidant Status of Red Blood Cells in Patients with Type 2 Diabetes and in Patients with Complications of Diabetes and Its Comparison with That of Healthy Subjects

2021 ◽  
Vol 10 (11) ◽  
pp. 825-828
Author(s):  
Chethan Subramanya
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Healthy Subjects ◽  
Diabetic Patients ◽  
Type 2 Diabetics ◽  
Total Antioxidant

BACKGROUND Oxidative stress plays a role in the pathogenesis of type 2 diabetes either by affecting insulin secretion or increasing insulin resistance. Oxidative stress is dealt by the body with the help of several antioxidant systems. The antioxidant levels in disorders causing oxidative stress such as diabetes mellitus are found to be low. Total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxide (GPx) levels are found to be depleted in diabetic groups when compared to control group comprising of healthy individuals. The activities of SOD and GPx are significantly low in diabetic patients. This study was conducted to determine the total antioxidant capacity and levels of antioxidants such as SOD and GPx in patients with type 2 diabetes and association between antioxidant levels and type 2 diabetics as well as type 2 diabetes with its associated complications. METHODS The study population comprised of 150 individuals, 50 subjects belonging to each subgroup of type 2 diabetics, and its associated complications and healthy subjects. The study was conducted in a tertiary care centre in Mangalore, Karnataka from September 2016 to December 2016. The antioxidant capacity of RBC, fasting blood levels of SOD and GPx were estimated by phospho molybdenum method. Nitroblue tetrazolium (NBT) reduction method and Rotruck method were also used. RESULTS The total antioxidant capacity, SOD and GPx were lower among diabetics and diabetic patients with complications as compared to normal nondiabetic subjects. CONCLUSIONS There is no significant difference in oxidative stress between patients with and without chronic complications of diabetes. KEY WORDS Antioxidant Levels, Glutathione Peroxidase, Oxidative Stress, Superoxide Dismutase, Nitroblue tetrazolium Reduction

Assessment of Superoxide Dismutase and Catalase Evolution in Different Stages of an Experimental Endometriosis

2017 ◽  
Vol 68 (6) ◽  
pp. 1381-1383
Author(s):  
Allia Sindilar ◽  
Carmen Lacramioara Zamfir ◽  
Eusebiu Viorel Sindilar ◽  
Alin Constantin Pinzariu ◽  
Eduard Crauciuc ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Reproductive Function ◽  
Female Rats ◽  
Oxygen Species ◽  
Efficient Treatment ◽  
Endometrial Cells ◽  
Gynecological Disorder ◽  
The Impact

Endometriosis is described as a gynecological disorder characterized by the presence of endometrial tissue outside the uterus; extensively explored because of its increasing incidency, with an indubitable diagnostic only after invasive surgery, with no efficient treatment, it has still many aspects to be elucidated. A growing body of facts sustain oxidative stress as a crucial factor between the numerous incriminated factors implicated in endometriosis ethiopathogeny. Reactive oxygen species(ROS) act to decline reproductive function. Our study intends to determine if an experimental model of endometriosis may be useful to assess the impact of oxidative stress on endometrial cells; we have used a murine model of 18 adult Wistar female rats. A fragment from their left uterine horn was implanted in the abdominal wall. After 4 weeks, a laparatomy was performed, 5 endometrial implants were removed, followed by biochemical tissue assay of superoxide dismutase(SOD) and catalase(CAT). At the end of the experiment, the rats were sacrificed, the implants were removed for histopathological exam and biochemical assay of antioxidant enzymes. The results revealed decreased levels of antioxidant enzymes, pointing on significant oxidative stress involvement.

Evidences of Brain Plasticity and Motor Control Modulation after Hemodialysis Session by Helixone Membrane: BOLD-fMRI Study

2020 ◽  
Vol 19 (6) ◽  
pp. 466-477
Author(s):  
Saïd Boujraf ◽  
Rachida Belaïch ◽  
Abdelkhalek Housni ◽  
Badreeddine Alami ◽  
Tariq Skalli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Functional Activity ◽  
Brain Plasticity ◽  
Biological Assessment ◽  
Bold Fmri ◽  
Before And After ◽  
Total Antioxidant ◽  
Inflammatory State ◽  
Functional Control ◽  
The Impact

Objective: The aim of this paper is to demonstrate the impact of hemodialysis (HD) using synthetic Helixone membrane on brain functional control reorganization and plasticity in the cortical area generated while Oxidative Stress (OS) would be the main impacting agent. Methods: Indeed, 9 chronic HD patients underwent identical brain BOLD-fMRI assessment using the motor paradigm immediately before and after the same HD sessions. To assess the oxidative stress, the same patients underwent biological-assessment, including Malondialdehyde (MDA) and Total- Antioxidant-Activity (TAOA) reported in earlier papers. Results: BOLD-fMRI maps of motor areas obtained from HD-patients before and after HD sessions revealed a significant enhancement of activation volume of the studied motor cortex after HD reflecting brain plasticity. Results were correlated with OS assessed by the measurement of MDA and TAOA; this correlation was close to 1. Conclusion: Indeed, HD enhances the inflammatory state of brain tissues reflected by the increased OS. The functional brain reaction demonstrated a functional activity reorganization to overcome the inflammatory state and OS enhanced by HD process. This functional activity reorganization reveals brain plasticity induced by OS originated by HD.

The Impact of Concomitant Chronic Pancreatitis on Prooxidant-antioxidant Status and Other Conditions in Osteoarthritis

2016 ◽  
pp. 75-78
Author(s):  
Liliia Babynets ◽  
Tetiana Maevska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Chronic Pancreatitis ◽  
Functional Capacity ◽  
Antioxidant Status ◽  
Activation Parameters ◽  
Tissue Destruction ◽  
The Impact ◽  
Stress Accumulation

The study proved that patients with combined progress of osteoarthritis and chronic pancreatitis have reliable top-level activation of lipid peroxidation in terms of malonyc aldehyde and tissue destruction in terms of oxyproline, weakening of the antioxidant level (in terms of superoxide dismutase and SH-groups) and activation parameters of catalase and ceruloplasmin (p<0,05). The authentic predictority of patients biological age, duration of combined clinical courses, the functional capacity of the pancreas in terms of fecal α-elastase, structural state by ultrasound criteria for progression effects of oxidative stress, accumulation oxyproline activation parameters catalase and ceruloplasmin, which statistically was reflected by the presence of mainly moderate of significant correlations between these groups of indicators have been identified.

scholarly journals Decreased Oxidative Stress in Male Patients with Active Phase Ankylosing Spondylitis Who Underwent Whole-Body Cryotherapy in Closed Cryochamber

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Agata Stanek ◽  
Armand Cholewka ◽  
Tomasz Wielkoszyński ◽  
Ewa Romuk ◽  
Aleksander Sieroń
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Ankylosing Spondylitis ◽  
Antioxidant Status ◽  
Active Phase ◽  
Post Treatment ◽  
Whole Body ◽  
Male Patients ◽  
Whole Body Cryotherapy ◽  
The Impact

Objective. The aim of the study was to estimate the impact of whole body cryotherapy (WBC) on oxidative stress when performed in a closed cryochamber on patients with ankylosing spondylitis (AS). Material and methods. The effect of ten WBC procedures lasting 3 minutes a day with a subsequent 60-minute session kinesiotherapy on oxidative stress in male AS patients (WBC group n=16) was investigated. To assess the disease activity, the Bath Ankylosing Spondylitis Diseases Activity Index (BASDAI) and Bath Ankylosing Spondylitis Functional Index (BASFI) were calculated. The WBC group was compared to the kinesiotherapy only (KT; n=16) group. The routine parameters of oxidative stress (antioxidant enzymatic and nonenzymatic antioxidant status, lipid peroxidation products, total oxidative status (TOS), and oxidative stress index (OSI)) were estimated one day before the beginning and one day after the completion of the research program. Results. After the completion of the treatment in the WBC group, a significant decrease of oxidative stress markers (TOS and OSI) and a significant increase of total antioxidant status were observed. The erythrocyte activity of glutathione peroxidase, glutathione reductase decreased significantly in both groups, but the differences of activity of that enzymes prior to post treatment values (Δ) in the KT group were significantly higher as compared to the WBC group. The activity of erythrocyte catalase and plasma ZnCu isoenzyme of superoxide dismutase showed a decreased tendency; erythrocyte total superoxide dismutase activity showed an increased tendency in the WBC group after the completion of the treatment. The BASDAI and BASFI decreased significantly in both groups, but the differences of value indexes prior to post treatment (Δ) were significantly higher in the WBC than KT group. Conclusion. WBC performed in a closed cryochamber decreases oxidative stress and improves BASDAI and BASFI indexes in male patients during the active phase of ankylosing spondylitis.

Ameliorative Effects of Indole-3-Carbinol and/or Sulforaphane Against Gold Nanoparticle-Induced Cytotoxicity in Rats

2021 ◽  
Vol 13 (2) ◽  
pp. 222-234
Author(s):  
Maha I. Alkhalaf ◽  
Wafa S. Alansari
Keyword(s):  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Superoxide Dismutase ◽  
Creatine Kinase ◽  
Albino Rats ◽  
Functional Markers ◽  
Related Factor ◽  
Nuclear Factor ◽  
Glutathione S Transferase ◽  
Total Antioxidant

Gold nanoparticles (GNPs) are the most commonly used metal nanoparticles due to their promising characteristics. However, application of GNs in medical and biological fields has resulted in toxicity to several organs. Indole-3-carbinol (I3C) and sulforaphane (SF) are the two well-known natural compounds, largely present in cruciferous vegetables. This study aimed to explore the therapeutic efficacy of I3C and SF alone or in combination against GN-induced renal and cardiac toxicities. Fifty male Albino rats were randomly segregated into five groups with each group containing 10 rats; G1, control; G2, intraperitoneally administered with a suspension of GNPs (10 nm in size; 20 µg/kg body weight (b.w.) for 7 days; G3, GN-injected rats, supplemented with SF (5 mg/kg b.w) daily for 7 days; G4, GN-injected rats, supplemented orally with I3C (150 mg/kg b.w.) for 7 days and G5, GN-injected rats supplemented orally with SF and I3C daily for 7 days. GN treatment significantly disturbed kidney functional markers, as evidenced by significantly increased levels of urea, creatinine and creatine kinase. Additionally, GNs significantly increased renal and cardiac levels of malondialdehyde, 8-hydroxydeoxyguanosine and interleukin-6, and depleted, glutathione S-transferase, glutathione reductase, superoxide dismutase, total antioxidant capacity, and nuclear factor erythroid 2-related factor 2. In contrast, treatment with I3C and SF alone or in combination significantly restored all the parameters to their near normal levels. GN induced histological abnormalities were also significantly attenuated. Taken together, the data indicate that the SF and I3C are more effective when given separately than when given together in lowering GN-induced toxicity by their ability to alleviate oxidative stress and inflammation.

scholarly journals Basic Studies on the Oxidative Stress Markers in Two Types of Horse Breed: Semi-isolated Population of Huculs Is Different from Commercially Used Arabian Horses

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Barbara A. Bażanów ◽  
Elżbieta Chełmecka ◽  
Ewa Romuk ◽  
Dominika M. Stygar
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Animal Health ◽  
Oxidative Stress Markers ◽  
Serum Samples ◽  
Antioxidants Activity ◽  
Stress Markers ◽  
Total Antioxidant ◽  
Basic Studies ◽  
Oxidant Status

Hucul and Arabian horses differ in the physiological constitution and exposition to environmental conditions. Oxidative stress plays a pathogenic role in many diseases and enables further injuries. The objective of this study was to compare the levels of enzymatic and nonenzymatic oxidative stress markers in Hucul horses living in seminatural conditions and in commercially handled Arabian horses. We tested the serum samples for total superoxide dismutase (total SOD), Cu-Zn-superoxide dismutase (CuZnSOD), and Mn-dependent superoxide dismutase (MnSOD) activity; for lipofuscin (LPS), ceruloplasmin (CER) and malondialdehyde (MDA) concentration; and for total antioxidant capacity (TAC) and total oxidant status (TOS). Total SOD (p<0.001), MnSOD (p<0.001), and CuZnSOD (p<0.001) activities were significantly higher whereas LPS (p<0.05), TAC (p<0.001), TOS (p<0.001), and MDA (p<0.001) concentrations were significantly lower in the serum samples collected from Huculs vs. Arabian horses, regardless of the gender. Gender, regardless of the breed, had no significant impact on the antioxidants’ activity and concentration. Total SOD and MnSOD activities were significantly higher in Hucul’s mares when compared to Hucul’s stallions. Concentrations of TAC and TOS were significantly lower in Arabian stallions than in Arabian mares. Commercially handled horses expressed a higher level of oxidative stress markers in comparison to breeds living in seminatural conditions. We conclude that antioxidants are important biomarkers of animal health, whether they are under maintenance care or performing physical exercise.

Intrathymic and intrasplenic oxidative stress mediates thymocyte and splenocyte damage in acutely exercised mice

1999 ◽  
Vol 86 (6) ◽  
pp. 1823-1827 ◽  
Author(s):  
A. A. Azenabor ◽  
L. Hoffman-Goetz
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Acute Exercise ◽  
Lipid Peroxides ◽  
Membrane Lipid ◽  
Splenic Tissue ◽  
Lymphoid Tissues ◽  
Oxygen Species ◽  
The Impact

Reactive oxygen species may contribute to apoptosis in lymphoid tissues observed after exercise. Thymic and splenic tissues excised from control mice (C) or mice immediately after ( t 0) or 24 h after ( t 24) a run to exhaustion (RTE) were assayed for biochemical indexes of oxidative stress [thymic and splenic membrane lipid peroxides, superoxide dismutase, catalase, plasma uric acid (UA), and ascorbic acid (AA)]. There were significant increases in membrane lipid peroxides in thymus ( P < 0.001) and spleen ( P < 0.001) in acutely exercised mice relative to controls (thymus: C = 2.74 ± 0.80 μM; t 0 = 7.45 ± 0.48 μM; t 24 = 9.44 ±1.41 μM; spleen: C = 0.48 ± 0.22 μM; t 0 = 1.78 ± 0.28 μM; t 24 = 2.81 ± 0.34 μM). The thymic and splenic tissue antioxidant enzymes concentrations of superoxide dismutase and catalase were significantly lower in samples collected at t 0 relative to C and t 24 mice ( P < 0.001). Plasma UA and AA levels were used to assess the impact of the RTE on the peripheral antioxidant pool. There was no significant change in UA levels and a significant reduction in plasma AA concentrations ( P < 0.001); the reduction in plasma AA occurred at t 24 (6.53 ± 1.64 μM) relative to t 0 (13.11 ± 0.71 μM) and C (13.26 ± 1.2 μM). These results suggest that oxidative damage occurs in lymphoid tissues after RTE exercise and that such damage may contribute to lymphocyte damage observed after acute exercise.

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