scholarly journals Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1259
Author(s):  
Dayun Yan ◽  
Alisa Malyavko ◽  
Qihui Wang ◽  
Kostya (Ken) Ostrikov ◽  
Jonathan H. Sherman ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Types ◽  
Atmospheric Plasma ◽  
Physical Factors ◽  
Bacterial Cells ◽  
Ionized Gas ◽  
Cold Atmospheric Plasma ◽  
Wide Range ◽  
Physical Effects ◽  
Underlying Mechanisms

Cold atmospheric plasma (CAP) is a near-room-temperature, partially ionized gas composed of reactive neutral and charged species. CAP also generates physical factors, including ultraviolet (UV) radiation and thermal and electromagnetic (EM) effects. Studies over the past decade demonstrated that CAP could effectively induce death in a wide range of cell types, from mammalian to bacterial cells. Viruses can also be inactivated by a CAP treatment. The CAP-triggered cell-death types mainly include apoptosis, necrosis, and autophagy-associated cell death. Cell death and virus inactivation triggered by CAP are the foundation of the emerging medical applications of CAP, including cancer therapy, sterilization, and wound healing. Here, we systematically analyze the entire picture of multi-modal biological destruction by CAP treatment and their underlying mechanisms based on the latest discoveries particularly the physical effects on cancer cells.

scholarly journals Cold Atmospheric Plasma Treatment of Chondrosarcoma Cells Affects Proliferation and Cell Membrane Permeability

2020 ◽  
Vol 21 (7) ◽  
pp. 2291
Author(s):  
Lyubomir Haralambiev ◽  
Andreas Nitsch ◽  
Josephine M. Jacoby ◽  
Silas Strakeljahn ◽  
Sander Bekeschus ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Membrane Permeability ◽  
Surgical Resection ◽  
Cell Lines ◽  
Atp Release ◽  
Cell Types ◽  
Atmospheric Plasma ◽  
Cell Membrane Permeability ◽  
Membrane Function ◽  
Cold Atmospheric Plasma

Chondrosarcoma is the second most common malign bone tumor in adults. Surgical resection of the tumor is recommended because of its resistance to clinical treatment such as chemotherapy and radiation therapy. Thus, the prognosis for patients mainly depends on sufficient surgical resection. Due to this, research on alternative therapies is needed. Cold atmospheric plasma (CAP) is an ionized gas that contains various reactive species. Previous studies have shown an anti-oncogenic potential of CAP on different cancer cell types. The current study examined the effects of treatment with CAP on two chondrosarcoma cell lines (CAL-78, SW1353). Through proliferation assay, the cell growth after CAP-treatment was determined. A strong antiproliferative effect for both cell lines was detected. By fluorescein diacetate (FDA) assay and ATP release assay, alterations in the cell membrane and associated translocation of low molecular weight particles through the cytoplasmic membrane were observed. In supernatant, the non-membrane-permeable FDA and endogenously synthesized ATP detected suggest an increased membrane permeability after CAP treatment. Similar results were shown by the dextran-uptake assay. Furthermore, fluorescence microscopic G-/F-actin assay was performed. G- and F-actin were selectively dyed, and the ratio was measured. The presented results indicate CAP-induced changes in cell membrane function and possible alterations in actin-cytoskeleton, which may contribute to the antiproliferative effects of CAP.

scholarly journals On the Anti-Cancer Effect of Cold Atmospheric Plasma and the Possible Role of Catalase-Dependent Apoptotic Pathways

Cells ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 2330
Author(s):  
Charlotta Bengtson ◽  
Annemie Bogaerts
Keyword(s):  
Mathematical Model ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Cancer Cell Death ◽  
Anti Cancer ◽  
Apoptotic Pathways ◽  
Cell Signaling Pathways

Cold atmospheric plasma (CAP) is a promising new agent for (selective) cancer treatment, but the underlying cause of the anti-cancer effect of CAP is not well understood yet. Among different theories and observations, one theory in particular has been postulated in great detail and consists of a very complex network of reactions that are claimed to account for the anti-cancer effect of CAP. Here, the key concept is a reactivation of two specific apoptotic cell signaling pathways through catalase inactivation caused by CAP. Thus, it is postulated that the anti-cancer effect of CAP is due to its ability to inactivate catalase, either directly or indirectly. A theoretical investigation of the proposed theory, especially the role of catalase inactivation, can contribute to the understanding of the underlying cause of the anti-cancer effect of CAP. In the present study, we develop a mathematical model to analyze the proposed catalase-dependent anti-cancer effect of CAP. Our results show that a catalase-dependent reactivation of the two apoptotic pathways of interest is unlikely to contribute to the observed anti-cancer effect of CAP. Thus, we believe that other theories of the underlying cause should be considered and evaluated to gain knowledge about the principles of CAP-induced cancer cell death.

scholarly journals Resistance of L. monocytogenes and S. Typhimurium towards Cold Atmospheric Plasma as Function of Biofilm Age

2018 ◽  
Vol 8 (12) ◽  
pp. 2702 ◽  
Author(s):  
Marlies Govaert ◽  
Cindy Smet ◽  
Maria Baka ◽  
Branimir Ećimović ◽  
James L. Walsh ◽  
...  
Keyword(s):  
Cell Density ◽  
Atmospheric Plasma ◽  
Inactivation Kinetics ◽  
Bacterial Cells ◽  
Cold Atmospheric Plasma ◽  
Complete Inactivation ◽  
Innovative Methods ◽  
Food Contact Surfaces ◽  
Different Ages ◽  
The One

The biofilm mode of growth protects bacterial cells against currently applied disinfection methods for abiotic (food) contact surfaces. Therefore, innovative methods, such as Cold Atmospheric Plasma (CAP), should be investigated for biofilm inactivation. However, more knowledge is required concerning the influence of the biofilm age on the inactivation efficacy in order to comment on a possible application of CAP in the (food) processing industry. L. monocytogenes and S. Typhimurium biofilms with five different ages (i.e., 1, 2, 3, 7, and 10 days) were developed. For the untreated biofilms, the total biofilm mass and the cell density were determined. To investigate the biofilm resistance towards CAP treatment, biofilms with different ages were treated for 10 min and the remaining cell density was determined. Finally, for the one-day old reference biofilms and the most resistant biofilm age, complete inactivation curves were developed to examine the influence of the biofilm age on the inactivation kinetics. For L. monocytogenes, an increased biofilm age resulted in (i) an increased biomass, (ii) a decreased cell density prior to CAP treatment, and (iii) an increased resistance towards CAP treatment. For S. Typhimurium, similar results were obtained, except for the biomass, which was here independent of the biofilm age.

scholarly journals Cold Atmospheric Plasma induces accumulation of lysosomes and caspase-independent cell death in U373MG glioblastoma multiforme cells

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gillian E. Conway ◽  
Zhonglei He ◽  
Ana Lacramioara Hutanu ◽  
George Paul Cribaro ◽  
Eline Manaloto ◽  
...  
Keyword(s):  
Cell Death ◽  
Glioblastoma Multiforme ◽  
Plasma Treatment ◽  
Acridine Orange ◽  
Molecular Mechanisms ◽  
Confocal Imaging ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Rapid Accumulation ◽  
Lysosomal Accumulation

Abstract Room temperature Cold Atmospheric Plasma (CAP) has shown promising efficacy for the treatment of cancer but the exact mechanisms of action remain unclear. Both apoptosis and necrosis have been implicated as the mode of cell death in various cancer cells. We have previously demonstrated a caspase-independent mechanism of cell death in p53-mutated glioblastoma multiforme (GBM) cells exposed to plasma. The purpose of this study was to elucidate the molecular mechanisms involved in caspase-independent cell death induced by plasma treatment. We demonstrate that plasma induces rapid cell death in GBM cells, independent of caspases. Accumulation of vesicles was observed in plasma treated cells that stained positive with acridine orange. Western immunoblotting confirmed that autophagy is not activated following plasma treatment. Acridine orange intensity correlates closely with the lysosomal marker Lyso TrackerTM Deep Red. Further investigation using isosurface visualisation of confocal imaging confirmed that lysosomal accumulation occurs in plasma treated cells. The accumulation of lysosomes was associated with concomitant cell death following plasma treatment. In conclusion, we observed rapid accumulation of acidic vesicles and cell death following CAP treatment in GBM cells. We found no evidence that either apoptosis or autophagy, however, determined that a rapid accumulation of late stage endosomes/lysosomes precedes membrane permeabilisation, mitochondrial membrane depolarisation and caspase independent cell death.

scholarly journals Cold Atmospheric Plasma Cancer Treatment, a Critical Review

2021 ◽  
Vol 11 (16) ◽  
pp. 7757
Author(s):  
Dayun Yan ◽  
Alisa Malyavko ◽  
Qihui Wang ◽  
Li Lin ◽  
Jonathan H. Sherman ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Molecular Mechanisms ◽  
Critical Evaluation ◽  
Atmospheric Plasma ◽  
Physical Factors ◽  
Atmospheric Conditions ◽  
Cold Atmospheric Plasma ◽  
Anti Cancer ◽  
Biological Impacts ◽  
Indirect Treatment

Cold atmospheric plasma (CAP) is an ionized gas, the product of a non-equilibrium discharge at atmospheric conditions. Both chemical and physical factors in CAP have been demonstrated to have unique biological impacts in cancer treatment. From a chemical-based perspective, the anti-cancer efficacy is determined by the cellular sensitivity to reactive species. CAP may also be used as a powerful anti-cancer modality based on its physical factors, mainly EM emission. Here, we delve into three CAP cancer treatment approaches, chemically based direct/indirect treatment and physical-based treatment by discussing their basic principles, features, advantages, and drawbacks. This review does not focus on the molecular mechanisms, which have been widely introduced in previous reviews. Based on these approaches and novel adaptive plasma concepts, we discuss the potential clinical application of CAP cancer treatment using a critical evaluation and forward-looking perspectives.

scholarly journals NO2-and NO3-enhance cold atmospheric plasma induced cancer cell death by generation of ONOO-

AIP Advances ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 105219 ◽  
Author(s):  
Dehui Xu ◽  
Qingjie Cui ◽  
Yujing Xu ◽  
Zhijie Liu ◽  
Zeyu Chen ◽  
...  
Keyword(s):  
Cell Death ◽  
Cancer Cell ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
Cancer Cell Death

scholarly journals Possible Mechanism of Glucose Uptake Enhanced by Cold Atmospheric Plasma: Atomic Scale Simulations

Plasma ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Jamoliddin Razzokov ◽  
Maksudbek Yusupov ◽  
Annemie Bogaerts
Keyword(s):  
Glucose Uptake ◽  
Biomedical Applications ◽  
Atomic Scale ◽  
Atmospheric Plasma ◽  
Oxidized Lipids ◽  
Free Energy Barrier ◽  
Diabetes Therapy ◽  
Cold Atmospheric Plasma ◽  
Underlying Mechanisms ◽  
Intracellular Glucose

Cold atmospheric plasma (CAP) has shown its potential in biomedical applications, such as wound healing, cancer treatment and bacterial disinfection. Recent experiments have provided evidence that CAP can also enhance the intracellular uptake of glucose molecules which is important in diabetes therapy. In this respect, it is essential to understand the underlying mechanisms of intracellular glucose uptake induced by CAP, which is still unclear. Hence, in this study we try to elucidate the possible mechanism of glucose uptake by cells by performing computer simulations. Specifically, we study the transport of glucose molecules through native and oxidized membranes. Our simulation results show that the free energy barrier for the permeation of glucose molecules across the membrane decreases upon increasing the degree of oxidized lipids in the membrane. This indicates that the glucose permeation rate into cells increases when the CAP oxidation level in the cell membrane is increased.

scholarly journals Influence of Cold Atmospheric Plasma on Acinetobacter baumannii

2019 ◽  
Vol 16 (1) ◽  
pp. 0151 ◽  
Author(s):  
Atta Et al.
Keyword(s):  
Atmospheric Plasma ◽  
Bacterial Cells ◽  
Standard Strain ◽  
Important Conclusion ◽  
Bacterial Colony ◽  
Cold Atmospheric Plasma ◽  
Sds Page ◽  
Bacterial Morphology ◽  
The Impact ◽  
Sequencing Result

baumannii is an aerobic gram negative coccobacilli, it is considered multidrug resistance pathogen (MDR) and causes several infections that are difficult to treat. This study is aims to employ physical methods in sterilization and inactivation of A. baumannii, as an alternative way to reduce the using of drugs and antibiotics.             Cold Atmospheric Plasma was generated by one electrode at 20KV, 4 power supply and distance between electrode and sample was fixed on 1mm. A. baumannii (ATCC 19704 and HHR1) were exposed to  Dielectric Barrier Discharge type of Cold Atmospheric Plasma (DBD-CAP) for several periods of time (15, 30, 45, and 60 sec.) . After sterilization test, several methods were done to analyze the effect of DBD-CAP on bacterial morphology, proteins and DNA. Change in morphology was assessed by cover slid method. Damaged DNA was investigated by PCR technique, and DNA sequencing. The impact of DBD-CAP on the entity of proteins was detected by SDS-PAGE. The observed inactivation of bacterial colony on agar plates has been quantified by measuring the inactivation diameter. The important conclusion that HHR1 more resistance to DBD-CAP than ATCC 17904 because it is more virulence than standard strain; thus, the growth of both strains is largely affected by plasma and this influence is increased by increasing the time of exposure, also the plasma affects the DNA especially on standard strain as it is explained in sequencing result, so it causes more deletion in DNA sequence. In addition, plasma also has been showed to damage proteins and morphology thus, the bacterial cells transform from cocco-bacillus to bacillus.

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