scholarly journals Functionality of Films from Nigella sativa Defatted Seed Cake Proteins Plasticized with Grape Juice: Use in Wrapping Sweet Cherries

Coatings ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1383
Author(s):  
Dana Yaseen ◽  
Mohammed Sabbah ◽  
Asmaa Al-Asmar ◽  
Mohammad Altamimi ◽  
Michela Famiglietti ◽  
...  

The main aim of this work is to improve the functionality of Nigella sativa protein concentrate (NSPC) films by using grape juice (GJ). The film’s mechanical, antioxidant, and antimicrobial activities were evaluated. The obtained results showed, for the first time, that GJ at concentrations of 2%–10% (v/v) are able to act as plasticizer for the NSPC films with promising film properties. The results showed that the tensile strength and Young’s modulus of NSPC films were reduced significantly when the GJ increased. However, the NSPC films prepared with 6% GJ observed a higher elongation at break compared with other films. Moreover, the obtained films showed very interesting and promising results for their antioxidant and antimicrobial properties compared with the control films. The sweet cherries wrapped with NSPC film showed that the TSS (Brix) was significantly lower compared to the control, after 10 days of storage. However, the titratable acidity, pH value, and L* of all cherries, either wrapped or not, was not significantly different in all storage times. On the other hand, hue angle was significantly lower after 10 days of storage at −18 °C compared with control films. GJ has a multi-functional effect for protein-based films as plasticizer, antioxidant, and antimicrobial function.

2021 ◽  
Author(s):  
Danijela Starčevic ◽  
◽  
Tatjana Jovanovic-Cvetkovic ◽  

The latest generations of interspecies grapevine hybrids are increasingly attracting the attention of producers, especially due to increased production safety and reduced use of pesticides. The economic effect is evident, and the obtained product follows modern world trends in the production of safe food. The aim of this paper is to examine the technological characteristics of three varieties of Morava, Johanniter and Riesling Rhine in the conditions of the Banja Luka region. Varieties Morava and Johanniter belong to the group of interspecies hybrids. The mentioned varieties were compared with the Riesling Rhine variety, which belongs to the varieties of the noble vine and represents the standard for the comparison of the given varieties. The focus of the research was on examining the yield of varieties, the characteristics of grapes and berries, as well as the quality of grapes. Elements of the mechanical composition of grapes and berries were made according to the method of Prostoserd (1946), and the characteristics of grape juice (% Brix, titratable acidity and pH value) according to official OIV methods. The variety Johanniter had the lowest weight of grapes (73.85 g), and the variety Morava had the highest weight (128.61 g). The varieties Johanniter and Riesling Rhine had a relatively high sugar content in grape juice (22.1%), while the variety Morava had a high sugar content (25.93%).


2020 ◽  
Vol 859 ◽  
pp. 226-231
Author(s):  
Sirikarn Pengon ◽  
Nawinda Chinatangkul ◽  
Chutima Limmatvapirat ◽  
Sontaya Limmatvapirat

Nelumbo nucifera is a medicinal herb widely distributed in Thailand which possesses its antioxidant and antimicrobial activities. The aims of this study were to investigate the antimicrobial effects and to develop the formulations containing Nelumbo nucifera crude extracts. The crude extracts of each part of the plants, petals, pollens, receptacles and stalks were separately obtained using 95% w/w ethyl alcohol and then subjected to the phytochemical studies. The total phenolic content was quantified by using Folin-ciocalteu colorimetric method, and the antibacterial features against Staphylococcus aureus was also evaluated. The nanoemulsions incorporated with Nelumbo nucifera crude extracts were thereafter formulated. The results showed that saponins, terpenoids, flavonoids and coumarins found in petals was 184.30 ± 0.50 mg GAE/g total phenolic content which was 4-fold higher than those found in the other part of the plants. Moreover, the crude extract from petals exhibited the activity against S. aureus as indicated by the minimum bactericidal concentration (MBC) of 100 mg/mL. Moreover, the good stability of nanoemulsions consisting of 0.05% w/w petal extract was observed displaying a particle size of 400 ± 139 nm, a zeta potential of -8.09 ± 1.04 mV, and a pH value of 5.87 ± 0.07. Owing to the antimicrobial test against S.aureus, the inhibition percentage of nanoemulsions was 80.35 ± 0.106. Accordingly, this study provides the increasing of antimicrobial properties of Nelumbo nucifera crude extract when preparing into the nanoemulsions formulation. In addition, this finding could raise the value of Nelumbo nucifera which could be utilized as an alternative active ingredient for the treatment of skin bacterial infection.


2021 ◽  
pp. 108201322098310
Author(s):  
Noelia Castillejo ◽  
Ginés Benito Martínez-Hernández ◽  
Francisco Artés-Hernández

The effect of revalorized Bimi leaves (B) and/or mustard (M) addition, as supplementary ingredients, to develop an innovative kale (K) pesto sauce was studied. Microbial, physicochemical (color, total soluble solids content -SSC-, pH and titratable acidity –TA-) and sensory quality were studied during 20 days at 5 °C. Bioactive compounds changes (total phenolics, total antioxidant capacity and glucoraphanin contents) were also monitored throughout storage. The high TA and pH changes in the last 6 days of storage were avoided in the K+B pesto when adding mustard, due to the antimicrobial properties of this brassica seed. SSC was increased when B + M were added to the K pesto, which positively masked the kale-typical bitterness. Mustard addition hardly change yellowness of the K pesto, being not detected in the sensory analyses, showing K+B+M pesto the lowest color differences after 20 days of shelf life. The addition of Bimi leaves to the K pesto enhanced its phenolic content while mustard addition did not negatively affect such total antioxidant compounds content. Finally, mustard addition effectively aimed to glucoraphanin conversion to its bioactive products. Conclusively, an innovative kale pesto supplemented with Bimi by-products was hereby developed, being its overall quality well preserved up to 20 days at 5 °C due to the mustard addition.


Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 137
Author(s):  
Xinzhen Fan ◽  
L’Hocine Yahia ◽  
Edward Sacher

Microbes, including bacteria and fungi, easily form stable biofilms on many surfaces. Such biofilms have high resistance to antibiotics, and cause nosocomial and postoperative infections. The antimicrobial and antiviral behaviors of Ag and Cu nanoparticles (NPs) are well known, and possible mechanisms for their actions, such as released ions, reactive oxygen species (ROS), contact killing, the immunostimulatory effect, and others have been proposed. Ag and Cu NPs, and their derivative NPs, have different antimicrobial capacities and cytotoxicities. Factors, such as size, shape and surface treatment, influence their antimicrobial activities. The biomedical application of antimicrobial Ag and Cu NPs involves coating onto substrates, including textiles, polymers, ceramics, and metals. Because Ag and Cu are immiscible, synthetic AgCu nanoalloys have different microstructures, which impact their antimicrobial effects. When mixed, the combination of Ag and Cu NPs act synergistically, offering substantially enhanced antimicrobial behavior. However, when alloyed in Ag–Cu NPs, the antimicrobial behavior is even more enhanced. The reason for this enhancement is unclear. Here, we discuss these results and the possible behavior mechanisms that underlie them.


Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 449
Author(s):  
Camilo Gutiérrez-Jara ◽  
Cristina Bilbao-Sainz ◽  
Tara McHugh ◽  
Bor-Sen Chiou ◽  
Tina Williams ◽  
...  

The cracking of sweet cherries causes significant crop losses. Sweet cherries (cv. Bing) were coated by electro-spraying with an edible nanoemulsion (NE) of alginate and soybean oil with or without a CaCl2 cross-linker to reduce cracking. Coated sweet cherries were stored at 4 °C for 28 d. The barrier and fruit quality properties and nutritional values of the coated cherries were evaluated and compared with those of uncoated sweet cherries. Sweet cherries coated with NE + CaCl2 increased cracking tolerance by 53% and increased firmness. However, coated sweet cherries exhibited a 10% increase in water loss after 28 d due to decreased resistance to water vapor transfer. Coated sweet cherries showed a higher soluble solid content, titratable acidity, antioxidant capacity, and total soluble phenolic content compared with uncoated sweet cherries. Therefore, the use of the NE + CaCl2 coating on sweet cherries can help reduce cracking and maintain their postharvest quality.


2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Balungile Madikizela ◽  
Ashwell Rungano Ndhlala ◽  
Jeffrey Franklin Finnie ◽  
Johannes Van Staden

Respiratory ailments are major human killers, especially in developing countries. Tuberculosis (TB) is an infectious disease causing a threat to human healthcare. Many South African plants are used in the traditional treatment of TB and related symptoms, but there has not been a sufficient focus on evaluating their antimicrobial properties. The aim of this study was to evaluate the antimicrobial properties of plants used traditionally to treat TB and related symptoms against microorganisms (Klebsiella pneumoniae, Staphylococcus aureus,andMycobacterium aurumA+) associated with respiratory infections using the microdilution assay. Ten plants were selected based on a survey of available literature of medicinal plants used in South Africa for the treatment of TB and related symptoms. The petroleum ether, dichloromethane, 80% ethanol, and water extracts of the selected plants were evaluated for antibacterial activity. Out of 68 extracts tested from different parts of the 10 plant species, 17 showed good antimicrobial activities against at least one or more of the microbial strains tested, with minimum inhibitory concentration ranging from 0.195 to 12.5 mg/mL. The good antimicrobial properties ofAbrus precatorius, Terminalia phanerophlebia, Indigofera arrecta,andPentanisia prunelloidesauthenticate their traditional use in the treatment of respiratory diseases. Thus, further pharmacological and phytochemical analysis is required.


Proceedings ◽  
2020 ◽  
Vol 66 (1) ◽  
pp. 9
Author(s):  
Yoram Gerchman

Plants have been explored and used as sources for antimicrobial extract and compounds for many years, but galls—specialized structures forms on such by diversity of organisms—have been explored much less. Aphid galls host many insects in closed, humid and sugar rich environments for long periods. We have tested the antimicrobial properties of Slavum wertheimae aphid galls on Pistacia atlantica. Secondary metabolites were extracted from leaves and galls with organic solvents, and essential oils with Clevenger, and tested by disk diffusion assay and volatile effect on bacteria and fungi, respectively. The results demonstrated that gall extracts/essential oils had much stronger activity against the diversity of bacteria and fungi. The large diversity of galls suggest they could be explored as source for novel compounds.


Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 787
Author(s):  
Tshikala Eddie Lulamba ◽  
Ezekiel Green ◽  
Mahloro Hope Serepa-Dlamini

Entomopathogenic nematodes (EPNs) are known to be highly pathogenic to insect pests, due to their associated symbiotic bacteria, which produce virulence factors, exo-enzymes and other harmful secondary metabolites to conquer, kill, and degrade their insect hosts. However, these properties are not fully characterized. This study reports on the antimicrobial activities of Photorhabdus sp. strain ETL, symbiotically associated to an insect pathogenic nematode, Heterorhabditis zealandica, against human pathogenic bacteria and toxigenic fungi, as well as the non-targeted profiling of its secondary metabolites (SMs) using gas chromatography coupled to high-resolution time-of-flight mass spectrometry. Fatty acids including 3-eicosene, (E)-; 5-eicosene, (E)-; eicosene; 9-octadecenamide; undecanoic acid with shown antimicrobial activities were detected. This provided more insight on the composition and bioactivities of SMs produced by the Photorhabdus sp.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5991 ◽  
Author(s):  
Carla de Fatima Alves Nonato ◽  
Débora Odília Duarte Leite ◽  
Rafael Caldas Pereira ◽  
Aline Augusti Boligon ◽  
Jaime Ribeiro-Filho ◽  
...  

BackgroundFruit consumption is currently considered beyond the nutritional aspects because of the important roles in disease prevention and benefits to health. The objective of this study was to characterize the chemical profile and evaluate the antioxidant and antimicrobial properties of different fractions obtained from fruit pulps ofMauritia flexuosa(MFFs).MethodsInitially, chloroform, ethyl acetate and ethanol fractions were obtained from the pulps. Quantifications of total phenols and flavonoids were performed using the methods of Folin-Ciocalteu and complexation with aluminum chloride, respectively. Standard samples were used to identify and quantify phenolic acids and flavonoids using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). The antioxidant capacity of the fractions was verified by sequestration of the free radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and iron chelating activity. The antimicrobial activity was determined using the microdilution method and the modulating activity was determined using sub-inhibitory concentrations of the fractions in association with antibiotics.ResultsThe chemical analyzes revealed the presence of catechin, caffeic acid, rutin, orientin, quercetin, apigenin, luteolin and kaempferol, where all are present in the ethyl acetate fraction. The fractions exhibited moderate antioxidant and antimicrobial activities against Gram-positive andCandidastrains in addition to modulating the activity of conventional antibiotics. The most expressive result was obtained from the association of the chloroform fraction with cefotaxime, which produced a synergistic effect, reducing the minimum inhibitory concentration (MIC) of the antibiotic from 1,024 to 256 μg/mL.DiscussionThe fractions presented a constitution rich in phenolic compounds, especially flavonoids. The data obtained demonstrated that the fractions presented moderate antioxidant activity by acting both as primary and secondary antioxidants. The fractions presented antimicrobial and antibiotic potentiating activities, being the first record of modulating effect of fractions of this species against the studied microbial strains, but failed in modulating the activity of antifungal drugs, indicating that this plant has the potential to be used in the development of therapeutic alternatives against resistant bacteria. The constitution phenolic the fractions may be responsible for their pharmacological properties in vitro.


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