scholarly journals Identification and Characterization of HSP90 Gene Family Reveals Involvement of HSP90, GRP94, and Not TRAP1 in Heat Stress Response in Chlamys farreri

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1592
Author(s):  
Haitao Yu ◽  
Zujing Yang ◽  
Mingyi Sui ◽  
Chang Cui ◽  
Yuqing Hu ◽  
...  
Keyword(s):  
Protein Folding ◽  
Heat Stress ◽  
Signal Transmission ◽  
Chlamys Farreri ◽  
Environmental Response ◽  
Heat Stress Response ◽  
Reading Frame ◽  
Nerve Signal ◽  
Living Organisms ◽  
Shock Proteins

Heat shock proteins 90 (HSP90s) are a class of ubiquitous, highly conserved, and multi-functional molecular chaperones present in all living organisms. They assist protein folding processes to form functional proteins. In the present study, three HSP90 genes, CfHSP90, CfGRP94 and CfTRAP1, were successfully identified in the genome of Chlamys farreri. The length of CfHSP90, CfGRP94 and CfTRAP1 were 7211 bp, 26457 bp, and 28699 bp, each containing an open reading frame (ORF) of 2181 bp, 2397 bp, and 2181bp, and encoding proteins of 726, 798, and 726 amino acids, respectively. A transcriptomic database demonstrated that CfHSP90 and CfGRP94 were the primary functional executors with high expression during larval development and in adult tissues, while CfTRAP1 expression was low. Furthermore, all of the three CfHSP90s showed higher expression in gonads and ganglia as compared with other tissues, which indicated their probable involvement in gametogenesis and nerve signal transmission in C. farreri. In addition, under heat stress, the expressions of CfHSP90 and CfGRP94 were significantly up-regulated in the mantle, gill, and blood, but not in the heart. Nevertheless, the expression of CfTRAP1 did not change significantly in the four tested tissues. Taken together, in coping with heat stress, CfHSP90 and CfGRP94 could help correct protein folding or salvage damaged proteins for cell homeostasis in C. farreri. Collectively, a comprehensive analysis of CfHSP90s in C. farreri was conducted. The study indicates the functional diversity of CfHSP90s in growth, development, and environmental response, and our findings may have implications for the subsequent in-depth exploration of HSP90s in invertebrates.

scholarly journals Environment and HSP90 modulate MAPK stomatal developmental pathway

2018 ◽  
Author(s):  
Despina Samakovli ◽  
Tereza Tichá ◽  
Miroslav Ovečka ◽  
Ivan Luptovčiak ◽  
Veronika Zapletalová ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stomatal Density ◽  
Stress Conditions ◽  
Environmental Cues ◽  
Stomatal Development ◽  
Heat Stress Response ◽  
Tightly Coupled ◽  
Tolerance Response ◽  
Nucleocytoplasmic Distribution ◽  
Shock Proteins

ABSTRACTStomatal ontogenesis is a key element of plant adaptation aiming to control photosynthetic efficiency and water management under fluctuating environments 1,2,3. Development of stomata is guided by endogenous and environmental cues and is tightly coupled to overall plant growth 1,2,3. YODA signaling pathway is essential to stomatal lineage specification4,5,6 since it regulates the activities of transcription factors such as SPEECHLESS (SPCH)7,8,9,10. Heat-shock proteins 90 (HSP90s) are evolutionarily conserved molecular chaperones implicated in a broad range of signalling pathways being integrated in interaction networks with client proteins11,12,13,14. Herein, based on genetic, molecular, biochemical, and cell biological evidence we report that heat-stress conditions affect phosphorylation and deactivation of SPCH and modulate stomatal density. We show that genetic and physical interactions between HSP90s and YODA control stomatal patterning, distribution and morphology. We provide solid evidence that HSP90s play a major role in transducing the heat-stress response since they act upstream and downstream of YODA signalling, regulate the activity and nucleocytoplasmic distribution of MAPKs, and the activation of SPCH. Thus, HSPs control the stomatal development both under normal temperature and acute heat-stress conditions. Our results demonstrate that HSP90s couple stomatal formation and patterning to environmental cues providing an adaptive mechanism of heat-stress tolerance response and stomatal formation in Arabidopsis.

Mining Heat Stress Induced Genes Encoding Heat Shock Proteins (HSPs) and Heat Stress Transcription Factors (HSFs) in Strawberry (Fragaria x ananassa)

2013 ◽  
Vol 446-447 ◽  
pp. 936-941
Author(s):  
Lee Fong Lin ◽  
Shih Yi Liao ◽  
Jui Hung Yen ◽  
Shu Jiau Chiou ◽  
Cheng Yu Lee ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Proteins ◽  
Extreme Temperature ◽  
Rt Pcr ◽  
Reading Frame ◽  
Genes Encoding ◽  
Stress Responsive Genes ◽  
Heat Stress Proteins ◽  
Shock Proteins ◽  
Heat Stress Transcription Factors

Plants have developed a variety of responses to extreme temperature situation in order to minimize damage and maintain cellular homeostasis. Recent research has revealed the involvement of heat stress proteins in thermotolerance in Arabidopsis. However, it is still obscure in strawberry plants. To identify heat stress responsive genes in strawberry (Fragaria x ananassa), we constructed a Transcriptome for strawberry and it was compared to Unigene annotated in several available proteomics, including Nr, COG, KEGC, and Swissport. Nineteen candidates with complete open reading frame (ORF) of 191 predicted genes encoding HSPs or HSFs were further analyzed. RT-PCR assays and heat stress induction analysis for these potential HSPs and HSFs were performed. Our data demonstrate that the prediction of potential heat stress responsive genes from our created Transcriptome for HSPs and HSFs, respectively, correlates well with the subsequent experimental results, indicating the Transcriptome we constructed could be translated into other scientific use.

scholarly journals Transcriptional Basis for Differential Thermosensitivity of Seedlings of Various Tomato Genotypes

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 655
Author(s):  
Yangjie Hu ◽  
Sotirios Fragkostefanakis ◽  
Enrico Schleiff ◽  
Stefan Simm
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Stress Response ◽  
Elevated Temperatures ◽  
Heat Stress Response ◽  
Atp Production ◽  
Different Temperatures ◽  
Related Plant ◽  
Shock Proteins ◽  
Tomato Genotypes

Transcriptional reprograming after the exposure of plants to elevated temperatures is a hallmark of stress response which is required for the manifestation of thermotolerance. Central transcription factors regulate the stress survival and recovery mechanisms and many of the core responses controlled by these factors are well described. In turn, pathways and specific genes contributing to variations in the thermotolerance capacity even among closely related plant genotypes are not well defined. A seedling-based assay was developed to directly compare the growth and transcriptome response to heat stress in four tomato genotypes with contrasting thermotolerance. The conserved and the genotype-specific alterations of mRNA abundance in response to heat stress were monitored after exposure to three different temperatures. The transcripts of the majority of genes behave similarly in all genotypes, including the majority of heat stress transcription factors and heat shock proteins, but also genes involved in photosynthesis and mitochondrial ATP production. In turn, genes involved in hormone and RNA-based regulation, such as auxin- and ethylene-related genes, or transcription factors like HsfA6b, show a differential regulation that associates with the thermotolerance pattern. Our results provide an inventory of genes likely involved in core and genotype-dependent heat stress response mechanisms with putative role in thermotolerance in tomato seedlings.

scholarly journals Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10022
Author(s):  
Fang Ma ◽  
Lintong Luo
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Gene Family ◽  
Cold Water ◽  
Functional Characterization ◽  
Head Kidney ◽  
Rna Seq ◽  
Systematic Analysis ◽  
Heat Stress Response ◽  
Shock Proteins

Heat shock proteins (Hsps) play an important role in many biological processes. However, as a typical cold water fish, the systematic identification of Hsp70/110 gene family of rainbow trout (Oncorhynchus mykiss) has not been reported, and the role of Hsp70/110 gene in the evolution of rainbow trout has not been described systematically. In this study, bioinformatics methods were used to analyze the Hsp70/110 gene family of rainbow trout. A total of 16 hsp70/110 genes were identified and classified into ten subgroups. The 16 Hsp70/110 genes were all distributed on chromosomes 2, 4, 8 and 13. The molecular weight is ranged from 78.93 to 91.39 kD. Gene structure and motif composition are relatively conserved in each subgroup. According to RNA-seq analysis of rainbow trout liver and head kidney, a total of four out of 16 genes were significantly upregulated in liver under heat stress, and a total of seven out of 16 genes were significantly upregulated in head kidney. RT-qPCR was carried out on these gene, and the result were consistent with those of RNA-seq. The significantly regulated expressions of Hsp70/110 genes under heat stress indicats that Hsp70/110 genes are involved in heat stress response in rainbow trout. This systematic analysis provided valuable information about the diverse roles of Hsp70/110 in the evolution of teleost, which will contribute to the functional characterization of Hsp70/110 genes in further research.

scholarly journals Physiological and Transcripts Analyses Reveal the Mechanism by Which Melatonin Alleviates Heat Stress in Chrysanthemum Seedlings

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaojuan Xing ◽  
Yurong Ding ◽  
Jinyu Jin ◽  
Aiping Song ◽  
Sumei Chen ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Regulatory Networks ◽  
Molecular Mechanisms ◽  
Dry Weight ◽  
Carotenoid Biosynthesis ◽  
Antioxidant Enzyme Activities ◽  
Chlorophyll Metabolism ◽  
Heat Stress Response ◽  
Shock Proteins

Heat stress limits the growth and development of chrysanthemum seedlings. Although melatonin (MT) has been linked to the heat stress response in plants, research on the underlying molecular mechanisms is scarce. In this study, the regulatory networks of MT on heat stress in chrysanthemum seedlings were explored. Physiological measurements suggested that MT not only reduced malondialdehyde accumulation, hydrogen peroxide content, and superoxide anion free radical generation rate, but also significantly promoted osmotic regulation substance synthesis (proline and soluble protein), antioxidant accumulation (GSH and AsA), and the antioxidant enzyme activities (SOD, POD, CAT, and APX) in chrysanthemum leaves under heat stress. Furthermore, MT increased the fresh weight, dry weight, chlorophyll content, photosynthesis rate, and gas exchange indexes. Further, RNA-seq results revealed 33,497 and 36,740 differentially expressed genes in the S/Con and SMT/ConMT comparisons, respectively. The differences in the comparisons revealed that MT regulated heat shock transcription factors (HSFs) and heat shock proteins (HSPs), and the genes involved in Ca2+ signal transduction (CNGCs and CAM/CMLs), starch and sucrose metabolism (EDGL, BGLU, SuS, and SPS), hormone (PP2Cs, AUX/IAAs, EBFs, and MYC2), chlorophyll metabolism (HEMA and PORA), flavonoid biosynthesis (CHS, DFR, and FNS), and carotenoid biosynthesis (DXPS, GGDP, and PSY). MT effectively improved chrysanthemum seedling heat-resistance. Our study, thus, provides novel evidence of a gene network regulated by MT under heat stress.

Heat Stress-Induced DNA Damage

Acta Naturae ◽  
2016 ◽  
Vol 8 (2) ◽  
pp. 75-78 ◽  
Author(s):  
O. L. Kantidze ◽  
A. K. Velichko ◽  
A. V. Luzhin ◽  
S. V. Razin
Keyword(s):  
Dna Damage ◽  
Heat Stress ◽  
Nucleic Acid ◽  
Dna Integrity ◽  
Regulation Of Transcription ◽  
Protein Homeostasis ◽  
Heat Stress Response ◽  
Stress Effects ◽  
Mechanisms Of Formation ◽  
Shock Proteins

Although the heat-stress response has been extensively studied for decades, very little is known about its effects on nucleic acids and nucleic acid-associated processes. This is due to the fact that the research has focused on the study of heat shock proteins and factors (HSPs and HSFs), their involvement in the regulation of transcription, protein homeostasis, etc. Recently, there has been some progress in the study of heat stress effects on DNA integrity. In this review, we summarize and discuss well-known and potential mechanisms of formation of various heat stress-induced DNA damage.

scholarly journals RNA-Seq-Based Comparative Transcriptome Analysis Highlights New Features of the Heat-Stress Response in the Extremophilic Bacterium Deinococcus radiodurans

2019 ◽  
Vol 20 (22) ◽  
pp. 5603 ◽  
Author(s):  
Dong Xue ◽  
Wenzheng Liu ◽  
Yun Chen ◽  
Yingying Liu ◽  
Jiahui Han ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Deinococcus Radiodurans ◽  
Regulatory System ◽  
Hypothetical Protein ◽  
Stress Factors ◽  
Heat Stress Response ◽  
Heat Response ◽  
Shock Proteins ◽  
In Cells

Deinococcus radiodurans is best known for its extraordinary resistance to diverse environmental stress factors, such as ionizing radiation, ultraviolet (UV) irradiation, desiccation, oxidation, and high temperatures. The heat response of this bacterium is considered to be due to a classical, stress-induced regulatory system that is characterized by extensive transcriptional reprogramming. In this study, we investigated the key functional genes involved in heat stress that were expressed and accumulated in cells (R48) following heat treatment at 48 °C for 2 h. Considering that protein degradation is a time-consuming bioprocess, we predicted that to maintain cellular homeostasis, the expression of the key functional proteins would be significantly decreased in cells (RH) that had partly recovered from heat stress relative to their expression in cells (R30) grown under control conditions. Comparative transcriptomics identified 15 genes that were significantly downregulated in RH relative to R30, seven of which had previously been characterized to be heat shock proteins. Among these genes, three hypothetical genes (dr_0127, dr_1083, and dr_1325) are highly likely to be involved in response to heat stress. Survival analysis of mutant strains lacking DR_0127 (a DNA-binding protein), DR_1325 (an endopeptidase-like protein), and DR_1083 (a hypothetical protein) showed a reduction in heat tolerance compared to the wild-type strain. These results suggest that DR_0127, DR_1083, and DR_1325 might play roles in the heat stress response. Overall, the results of this study provide deeper insights into the transcriptional regulation of the heat response in D. radiodurans.

scholarly journals Toward the discovery of biological functions associated with the mechanosensor Mtl1p of Saccharomyces cerevisiae via integrative multi-OMICs analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nelson Martínez-Matías ◽  
Nataliya Chorna ◽  
Sahily González-Crespo ◽  
Lilliam Villanueva ◽  
Ingrid Montes-Rodríguez ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heat Stress ◽  
Stress Response ◽  
Yeast Cells ◽  
Metabolome Analysis ◽  
Signaling Mechanism ◽  
Heat Stress Response ◽  
Cell Wall Integrity Pathway ◽  
Enhanced Expression ◽  
Shock Proteins

AbstractFunctional analysis of the Mtl1 protein in Saccharomyces cerevisiae has revealed that this transmembrane sensor endows yeast cells with resistance to oxidative stress through a signaling mechanism called the cell wall integrity pathway (CWI). We observed upregulation of multiple heat shock proteins (HSPs), proteins associated with the formation of stress granules, and the phosphatase subunit of trehalose 6-phosphate synthase which suggests that mtl1Δ strains undergo intrinsic activation of a non-lethal heat stress response. Furthermore, quantitative global proteomic analysis conducted on TMT-labeled proteins combined with metabolome analysis revealed that mtl1Δ strains exhibit decreased levels of metabolites of carboxylic acid metabolism, decreased expression of anabolic enzymes and increased expression of catabolic enzymes involved in the metabolism of amino acids, with enhanced expression of mitochondrial respirasome proteins. These observations support the idea that Mtl1 protein controls the suppression of a non-lethal heat stress response under normal conditions while it plays an important role in metabolic regulatory mechanisms linked to TORC1 signaling that are required to maintain cellular homeostasis and optimal mitochondrial function.

Sign in / Sign up

Export Citation Format

Share Document