scholarly journals Lignin-Mediated Biosynthesis of ZnO and TiO2 Nanocomposites for Enhanced Antimicrobial Activity

2019 ◽  
Vol 3 (3) ◽  
pp. 90 ◽  
Author(s):  
Kanchan M. Samb-Joshi ◽  
Yogesh A. Sethi ◽  
Anuradha A. Ambalkar ◽  
Hiralal B. Sonawane ◽  
Suresh P. Rasale ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Tio2 Nanoparticles ◽  
Phase Formation ◽  
Visible Region ◽  
Xrd Analysis ◽  
Structural Defects ◽  
Precipitation Method ◽  
Zno Nps ◽  
Average Size

In this work, we report the synthesis of fragmented lignin (FL) assisted zinc oxide (ZnO) and titanium oxide (TiO2) nanocomposites. The fragmented lignin synthesized from biomass (sugarcane bagasse) was used as a template to generate the morphology and crystallite structure of metal oxide nanomaterial. The nanocomposites were synthesized by a simple precipitation method, wherein fragmented lignin is used in alkaline medium as a template. X-ray diffraction (XRD) analysis shows the phase formation of hexagonal wurtzite ZnO and mixed phase formation of TiO2 as rutile and anatase. The morphology was studied by using field emission scanning electron microscopy (FE-SEM) and high-resolution transmission electron microscopy (HRTEM). The FE-SEM of pristine ZnO nanocomposites showed a cluster of particles whereas FL–ZnO NPs showed self-aligned nanoparticles in the form of rod shaped having average size 30–70 nm. Pristine TiO2 nanoparticles showed clusters of particles and FL–TiO2 nanocomposites showed well crystalline 41nm size nanocomposites. The FL acts as a surfactant which restrict the cluster formations. The band gap determined by diffuse reflectance spectra is 3.10 eV and 3.20 eV for FL–ZnO and FL–TiO2 nanocomposites, respectively. Photoluminescence spectra of both nanocomposites showed structural defects in the visible region. Further, the antimicrobial activity of pristine ZnO and TiO2 nanoparticles, and FL–ZnO and FL–TiO2 nanocomposites against Escherichia coli (ATCC25922), Staphylococcus aureus (ATCC25923) were studied under UV-A (315-400 nm) (8W) for 30min.

scholarly journals Synthesis of ZnO Nanoparticles by Precipitation Method with Their Antibacterial Effect

2018 ◽  
Vol 16 (2) ◽  
pp. 117 ◽  
Author(s):  
Muhammad Fajri Romadhan ◽  
Nurgaha Edhi Suyatma ◽  
Fahim Muchammad Taqi
Keyword(s):  
Antimicrobial Activity ◽  
Particle Size ◽  
Average Particle Size ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Zinc Nitrate ◽  
Sem Analysis ◽  
Precipitation Method ◽  
Average Particle ◽  
Zno Nps

The aim of this study was to synthesize and characterize Zinc oxide nanoparticles (ZnO-NPs) prepared by precipitation method. Zinc nitrate and sodium hydroxide was used as starting materials with biopolymer pectin as capping agent. ZnO-NPs were synthesized at three levels of temperatures (60, 80 and 100 °C) without or with calcinations (500 °C). Particle size analyzer (PSA) analysis results showed that the samples without calcination (T60, T80 and T100) having an average particle size respectively 105.13, 78.53, and 76.43 nm, whereas at the samples by calcination (T60C, T80C and T100C) each have average particle size of 88.73, 44.30 and 543.77 nm. The results showed that preparation of ZnO-NPs by using heating at 80 °C followed with calcinations at 500 °C (T80C) produced the smallest size. T80C samples further were analyzed using XRD, SEM and the antimicrobial activity compared with the ZnO-NPs commercials. XRD analysis confirmed that ZnO-NPs were successfully obtained and have form of pure nanostructure. SEM analysis showed that ZnO-NPs obtained has a spherical shape. Furthermore, this ZnO-NPs (T80C) has a better antimicrobial activity compared than commercial ZnO-NPs in market.

scholarly journals Enhanced Photocatalytic Activity of Cu2O Cabbage/RGO Nanocomposites under Visible Light Irradiation

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1712
Author(s):  
Appusamy Muthukrishnaraj ◽  
Salma Ahmed Al-Zahrani ◽  
Ahmed Al Otaibi ◽  
Semmedu Selvaraj Kalaivani ◽  
Ayyar Manikandan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Thermo Gravimetric Analysis ◽  
Visible Region ◽  
Catalytic Performance ◽  
Precipitation Method ◽  
Gravimetric Analysis ◽  
X Ray Diffraction ◽  
X Ray ◽  
Dye Pollutants ◽  
Transmission Electron

Towards the utilization of Cu2O nanomaterial for the degradation of industrial dye pollutants such as methylene blue and methyl orange, the graphene-incorporated Cu2O nanocomposites (GCC) were developed via a precipitation method. Using Hummers method, the grapheme oxide (GO) was initially synthesized. The varying weight percentages (1–4 wt %) of GO was incorporated along with the precipitation of Cu2O catalyst. Various characterization techniques such as Fourier-transform infra-red (FT-IR), X-ray diffraction (XRD), UV–visible diffused reflectance (UV-DRS), Raman spectroscopy, thermo gravimetric analysis (TGA), energy-dispersive X-ray analysis (EDX), and electro chemical impedance (EIS) were followed for characterization. The cabbage-like morphology of the developed Cu2O and its composites were ascertained from field-emission scanning electron microscopy (FESEM) and high-resolution transmission electron microscopy (HR-TEM). In addition, the growth mechanism was also proposed. The results infer that 2 wt % GO-incorporated Cu2O composites shows the highest value of degradation efficiency (97.9% and 96.1%) for MB and MO at 160 and 220 min, respectively. Further, its catalytic performance over visible region (red shift) was also enhanced to an appreciable extent, when compared with that of other samples.

scholarly journals STUDY OF ANTIMICROBIAL ACTIVITY OF SILVER-DOPED HYDROXYAPATITE

2019 ◽  
pp. 243-245
Author(s):  
HIBU WAHID ◽  
ANJUVAN SINGH
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Xrd Analysis ◽  
Chemical Precipitation ◽  
Silver Concentration ◽  
Precipitation Method ◽  
Zone Of Inhibition

Objective: Hydroxyapatite (Hap) is an important material used for implants because of its structural similarity with calcium phosphate of the teeth and bones. Hap is mostly used in the situations where load-bearing implants are needed, such as in dental and orthopedic applications. Dental plaque is caused due to the bacteria which induces secondary caries through microleakage between the restoration and the tooth structure. Methods: An attempt has been made to provide materials with antibacterial activity by incorporating an antibacterial agent into the chemical mixture (Hap). This study delineates the process of synthesizing silver-doped Hap (Ca10Agx(PO4)6(OH)2) by wet chemical precipitation method and by varying the silver concentration (0.3%–0.5%). The synthesized silver-doped Hap was further characterized using Fourier transform infrared spectroscopy and X-ray diffraction (XRD) analysis. The antimicrobial activities were tested against Gram-negative bacteria Escherichia coli. Results: The maximum zone of inhibition against Escherichia coli was observed when equal concentration of Hydroxyapatite and Silver were mixed together i.e XAg=0.5% Zone of inhibition helps to determine the antimicrobial activity of silver-doped Hap. An increase in the zone of inhibition indicates the least growth of microorganisms which was observed at a silver concentration XAg=0.5%. Conclusions: Silver-doped Hap was synthesized successfully using the chemical precipitation method and was characterized using Fourier transform infrared spectroscopy and XRD analysis. It can be concluded that silver-doped Hap can be an excellent substitute material used as dental implant device.

scholarly journals Synthesis, Characterization, and Antimicrobial Activity of Magnesium-Doped Hydroxyapatite Suspensions

Nanomaterials ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 1295 ◽  
Author(s):  
Daniela Predoi ◽  
Simona Liliana Iconaru ◽  
Mihai Valentin Predoi ◽  
George E. Stan ◽  
Nicolas Buton
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Precipitation Method ◽  
Gram Positive ◽  
Gram Negative ◽  
Morphological Studies ◽  
Average Grain Size ◽  
Co Precipitation

Obtaining nanoscale materials has allowed for the miniaturization of components, which has led to the possibility of achieving more efficient devices with faster functions and much lower costs. While hydroxyapatite [HAp, Ca10(PO4)6(OH)2] is considered the most widely used material for medical applications in orthopedics, dentistry, and general surgery, the magnesium (Mg) is viewed as a promising biodegradable and biocompatible implant material. Furthermore, Mg is regarded as a strong candidate for developing medical implants due to its biocompatibility and antimicrobial properties against gram-positive and gram-negative bacteria. For this study, magnesium-doped hydroxyapatite (Ca10−xMgx (PO4)6 (OH)2, xMg = 0.1), 10MgHAp, suspensions were successfully obtained by an adapted and simple chemical co-precipitation method. The information regarding the stability of the nanosized 10MgHAp particles suspension obtained by ζ-potential analysis were confirmed for the first time by a non-destructive ultrasound-based technique. Structural and morphological studies of synthesized 10MgHAp were conducted by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy in attenuated total reflectance (ATR) mode and scanning electron microscopy (SEM). The XRD analysis of the 10MgHAp samples confirmed that a single crystalline phase associated to HAp with an average grain size about 93.3 nm was obtained. The FTIR-ATR spectra revealed that the 10MgHAp sample presented broader IR bands with less visible peaks when compared to a well-crystallized pure HAp. The SEM results evidenced uniform MgHAp nanoparticles with spherical shape. The antimicrobial activity of the 10MgHAp suspension against gram-positive strains (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212), gram-negative strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853), as well as a fungal strain (Candida albicans ATCC 90029) were evaluated.

scholarly journals Phytofabrication of Silver/Silver Chloride Nanoparticles Using Aqueous Leaf Extract of Oedera genistifolia: Characterization and Antibacterial Potential

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4382 ◽  
Author(s):  
Kunle Okaiyeto ◽  
Mike O. Ojemaye ◽  
Heinrich Hoppe ◽  
Leonard V. Mabinya ◽  
Anthony I. Okoh
Keyword(s):  
Electron Microscopy ◽  
Cytotoxic Effect ◽  
Leaf Extract ◽  
Silver Chloride ◽  
Xrd Analysis ◽  
Reducing Agent ◽  
X Ray ◽  
Aqueous Leaf Extract ◽  
Average Size ◽  
Silver Silver

In this present study, silver nanoparticles (Ag/AgCl NPs) were synthesized using an aqueous leaf extract of Oedera genistifolia as a reducing agent. The biosynthesized Ag/AgCl NPs was characterized by UV-visible spectrophotometry, transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). In addition, sequel to antibacterial assay, the cytotoxic effect of the phytofabricated Ag/AgCl NPs was assessed against the HeLa cell line (human cervix adenocarcinoma). The results of the characterization of the synthesized Ag/AgCl NPs indicate the successful synthesis using plant extract as a reducing agent, with UV-Vis spectra between 290–360 nm. TEM results showed that Ag/AgCl NPs was spherical in shape with an average size of 34.2 nm. EDX analysis revealed that the particles were predominantly composed of carbon, oxygen, chlorine, and silver, while FTIR identified major phytochemical compounds, which could be responsible for bio-reducing and capping potential. XRD analysis showed the crystallinity of Ag/AgCl NPs, with a face-centred cubic structure. The studied Ag/AgCl NPs had no cytotoxic effect on HeLa cells and exhibited antibacterial activity (minimum inhibitory concentration (MIC) 0.25–1 mg/mL; minimum bactericidal concentration (MBC) 2–16 mg/mL) against both the Gram-negative and Gram-positive bacteria investigated. Findings from this study suggest that this plant as a good candidate for producing new antibacterial drugs.

scholarly journals Effect of Biosynthesized ZnO Nanoparticles on Multi-Drug Resistant Pseudomonas Aeruginosa

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 260 ◽  
Author(s):  
Syed Ghazanfar Ali ◽  
Mohammad Azam Ansari ◽  
Mohammad A. Alzohairy ◽  
Mohammad N. Alomary ◽  
Mohammad Jalal ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Environmentally Friendly ◽  
Cost Effective ◽  
Multidrug Resistant ◽  
Zno Nps ◽  
Synthesis Of Nanoparticles ◽  
Transmission Electron ◽  
Average Size

Synthesis of nanoparticles using the plants has several advantages over other methods due to the environmentally friendly nature of plants. Besides being environmentally friendly, the synthesis of nanoparticles using plants or parts of the plants is also cost effective. The present study focuses on the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using the seed extract of Butea monsoperma and their effect on to the quorum-mediated virulence factors of multidrug-resistant clinical isolates of Pseudomonas aeruginosa at sub minimum inhibitory concentration (MIC). The synthesized ZnO NPs were characterized by different techniques, such as Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and transmission electron microscopy (TEM). The average size of the nanoparticles was 25 nm as analyzed by TEM. ZnO NPs at sub MIC decreased the production of virulence factors such as pyocyanin, protease and hemolysin for P. aeruginosa (p ≤ 0.05). The interaction of NPs with the P. aeruginosa cells on increasing concentration of NPs at sub MIC levels showed greater accumulation of nanoparticles inside the cells as analyzed by TEM.

scholarly journals Biodegradable Hybrid Nanocomposite of Chitosan/Gelatin and Green Synthesized Zinc Oxide Nanoparticles for Food Packaging

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1143 ◽  
Author(s):  
Santosh Kumar ◽  
Abhinab Mudai ◽  
Barnali Roy ◽  
Indra Bhusan Basumatary ◽  
Avik Mukherjee ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Food Packaging ◽  
Fruits And Vegetables ◽  
Nanocomposite Films ◽  
Hybrid Nanocomposite ◽  
Hybrid Films ◽  
Zno Nps ◽  
Gram Positive Bacteria ◽  
Average Size

In the context of emerging global concerns with synthetic plastic packaging, alternative natural biodegradable packaging materials are gaining increasing attention for food packaging applications. In this study, chitosan and gelatin nanocomposite hybrid films containing green synthesized zinc oxide (ZnO) nanoparticles (NPs) were developed and microstructural properties were studied. Antimicrobial activity of the developed films was evaluated using both Gram negative (Escherichia coli) and Gram positive bacteria (Staphylococcus aureus). Green synthesis protocol was used for the precipitation of ZnO NPs using fruit extract of Cassia fistula. The as-synthesized polyhedral ZnO NPs were in the range of 20–40 nm (average size ≈29 nm). Reinforcement with ZnO NPs in the hybrid films lead to improved thermal stability, elongation-at-break (EAB), and compactness properties. The developed films with 2% and 4% ZnO NPs showed a smooth, compact, and heterogeneous surface morphology compared to the control (chitosan-gelatin hybrid) films. Disc diffusion assays showed that the nanocomposite film had significant antimicrobial activity against E. coli. The developed hybrid nanocomposite films have potential to be developed as biodegradable alternative for postharvest packaging of fresh fruits and vegetables.

scholarly journals Bacterial Mediated Rapid and Facile Synthesis of Silver Nanoparticles and Their Antimicrobial Efficacy against Pathogenic Microorganisms

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2615
Author(s):  
Md. Amdadul Huq ◽  
Shahina Akter
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Field Emission ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Gram Positive ◽  
Tem Analysis ◽  
Gram Negative ◽  
X Ray

In the present study, silver nanoparticles (AgNPs), biosynthesized using culture supernatant of bacterial strain Paenarthrobacter nicotinovorans MAHUQ-43, were characterized and their antimicrobial activity was investigated against both Gram-positive Bacillus cereus and Gram-negative bacteria Pseudomonas aeruginosa. Bacterial-mediated synthesized AgNPs were characterized by UV-Visible (UV-Vis) spectrophotometer, field emission-transmission electron microscopy (FE-TEM), energy dispersive X-ray (EDX), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and dynamic light scattering (DLS) analysis. The UV-Vis spectral analysis showed the absorption maxima at 466 nm which assured the synthesis of AgNPs. The FE-TEM analysis revealed the spherical shape of nanoparticles with the size range from 13 to 27 nm. The EDX and XRD analysis ensured the crystalline nature of biosynthesized AgNPs. The FTIR analysis revealed the involvement of different biomolecules for the synthesis of AgNPs as reducing and capping agents. The bacterial-mediated synthesized AgNPs inhibited the growth of pathogenic strains B. cereus and P. aeruginosa and developed a clear zone of inhibition (ZOI). The MIC and MBC for both pathogens were 12.5 µg/mL and 25 µg/mL, respectively. Moreover, field emission scanning electron microscopy analysis revealed that the synthesized AgNPs can destroy the outer membrane and alter the cell morphology of treated pathogens, leading to the death of cells. This study concludes the eco-friendly, facile and rapid synthesis of AgNPs using P. nicotinovorans MAHUQ-43 and synthesized AgNPs showed excellent antimicrobial activity against both Gram-positive and Gram-negative pathogens.

scholarly journals A Comprehensive Study on the Effect of ZnO and ZnAl2O4 Nanoparticles on the Mechanical Properties of Epoxy Coating: Tensile and Hardness

2021 ◽  
Vol 15 (5) ◽  
pp. 45
Author(s):  
Abeer Shmait ◽  
Nour El Ghouch ◽  
J. Al Boukhari ◽  
A. M. Abdel-Gaber ◽  
R. Awad
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Tensile Tests ◽  
Precipitation Method ◽  
Zno Nps ◽  
Co Precipitation

ZnO and ZnAl2O4 nanoparticles (NPs) were successfully prepared by the co-precipitation method and characterized by x-ray powder diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The prepared NPs were incorporated into epoxy (EP) coating with mass ratios 200  800 mg/kg of ZnO NPs/EP and ZnAl2O4 NPs /EP. The prepared coatings were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy, and their mechanical properties were investigated, at room temperature, after 5, 10, 15, and 20 days of preparation. Tensile tests showed an improvement in the tensile properties, with the best improvement in ultimate tensile strength (93.2%) for 800 mg/kg ZnAl2O4 NPs/EP coating after 15 days of preparation. The ZnO NPs/EP and ZnAl2O4 NPs/EP coatings exhibited noticeable sensitivity to the stretching rate. Vickers microhardness (Hv) investigations showed normal indentation size effect behavior for all the samples. The best improvement in Hv was attained after 5 days of preparation, for all coatings, with the best improvement (9.15%) for 700 mg/kg ZnO NPs/EP.

scholarly journals Synthesis and growth of spherical ZnO nanoparticles using different amount of plant extract

2021 ◽  
Vol 24 (12) ◽  
pp. 2147-2151
Author(s):  
Y.A. Dallatu ◽  
G.A. Shallangwa ◽  
S.N. Africa
Keyword(s):  
Electron Microscopy ◽  
Plant Extract ◽  
Zno Nanoparticles ◽  
Precipitation Method ◽  
Zno Nps ◽  
Structural Morphology ◽  
Uniform Particles ◽  
Transmission Electron ◽  
Seed Husk ◽  
Co Precipitation

The use of plant extracts has become an interesting ecofriendly method to synthesize and stabilize the different structures nanoparticles (NPs). This work investigated the effect of plant extract as a reducing and stabilizing agent on the growth and morphology of ZnO nanoparticles (ZnO-NPs). Green synthesis and growth of spherical ZnONPs was carried out by co-precipitation method using a Zinc acetate salt and various amounts of Azadirachta indica seed husk extract (20 ml and 40 ml). The synthesized ZnO-NPs were characterized by Fourier transform infrared (FTIR), scanning electron microscopy (SEM-EDX), and transmission electron microscopy (TEM). The FTIR analyses revealed the presence of Phenolic alcohol, amines and carboxylic acid groups and ZnO in synthesized NPs with more intense peaks at higher amount (40 ml) of A. indica extract. Also, structural morphology analyses using SEM revealed uniform spherical shaped particles with diameter from 25 to 60 nm (20 ml of extract) and 19 to 35 nm (40 ml of extract) for ZnO-NPs. The EDX spectral revealed that the required phase of Zn and O was present 69.54% (Zn) and 30.46% (O) at 20 ml of extract, also 73.71% (Zn), 26.26% (O) at 40 ml of extract respectively and confirmed high purity for the synthesized ZnO NPs. TEM revealed spherical shaped NPs with diameter ranging from 28 to 52 nm (20 ml of extract) and 8.2 to 11.9 nm (40 ml of extract) respectively, with a trend reduction in particle size of NPs at higher amount of A. indica seed extract (40 ml) and growth of more uniform particles with no agglomeration. The study showed successful growth of spherical ZnO-NPs with required properties at a higher amount of extract.

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