scholarly journals Chitosomes-In-Chitosan Hydrogel for Acute Skin Injuries: Prevention and Infection Control

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 269
Author(s):  
Lisa Myrseth Hemmingsen ◽  
Kjersti Julin ◽  
Luqman Ahsan ◽  
Purusotam Basnet ◽  
Mona Johannessen ◽  
...  
Keyword(s):  
Water Solubility ◽  
Treatment Options ◽  
Antimicrobial Effect ◽  
The Novel ◽  
Chitosan Hydrogel ◽  
Skin Injuries ◽  
Lipid Nanocarriers ◽  
Treatment Of Infection ◽  
Skin Retention ◽  
Timely Treatment

Burns and other skin injuries are growing concerns as well as challenges in an era of antimicrobial resistance. Novel treatment options to improve the prevention and eradication of infectious skin biofilm-producing pathogens, while enhancing wound healing, are urgently needed for the timely treatment of infection-prone injuries. Treatment of acute skin injuries requires tailoring of formulation to assure both proper skin retention and the appropriate release of incorporated antimicrobials. The challenge remains to formulate antimicrobials with low water solubility, which often requires carriers as the primary vehicle, followed by a secondary skin-friendly vehicle. We focused on widely used chlorhexidine formulated in the chitosan-infused nanocarriers, chitosomes, incorporated into chitosan hydrogel for improved treatment of skin injuries. To prove our hypothesis, lipid nanocarriers and chitosan-comprising nanocarriers (≈250 nm) with membrane-active antimicrobial chlorhexidine were optimized and incorporated into chitosan hydrogel. The biological and antibacterial effects of both vesicles and a vesicles-in-hydrogel system were evaluated. The chitosomes-in-chitosan hydrogel formulation demonstrated promising physical properties and were proven safe. Additionally, the chitosan-based systems, both chitosomes and chitosan hydrogel, showed an improved antimicrobial effect against S. aureus and S. epidermidis compared to the formulations without chitosan. The novel formulation could serve as a foundation for infection prevention and bacterial eradication in acute wounds.

Primordial science and pioneering approaches of guggul for chronic ailments – A modernized review

2020 ◽  
Vol 7 (2) ◽  
pp. 14-20
Author(s):  
Jamal Basha D ◽  
Kumar P R ◽  
Ranganayakulu D
Keyword(s):  
Water Solubility ◽  
Therapeutic Potential ◽  
Chemical Constituents ◽  
Signs And Symptoms ◽  
Dosage Forms ◽  
The Novel ◽  
Lipids Metabolism ◽  
Clinical Conditions ◽  
Novel Drug ◽  
Conventional Dosage

An oleo gum resin guggulu is a product which obtained as a result of gummosis from the bark of Commiphora wightii (Arnott) Bhandari [syn. Commiphoramukul (Hook. Ex Stocks) Family, Burseraceae]. It has been known for its immense applicability in the Ayurveda since time immemorial for the treatment of variety of disorders such as inflammation, gout, rheumatism, impotence, leprosy, obesity, and disorders of lipids metabolism. It is a mixture of phytoconstituents like terpenoids, steroids, flavonoids, guggultetrols, lignans, sugars, and amino acids. This review is an effort to compile all the information available on all of its chemical constituents which are responsible for its therapeutic potential, limitation of guggul extracts and the necessity of novel principles for gum guggul. Nowadays, Guggul is available as the marketed formulation for curing numerous clinical conditions and is accessible in combination with various other ingredients. Though conventional dosage form shows the dominance as patient compliance and easy availability, yet it has found to pose the problems like dose fluctuation, peak-valley effect, non-adjustment of the administered drug, invasiveness etc. Guggul lacks its desired effect due to its low bioavailability and water solubility. This makes it a partial or a deficient therapy for remedy of many signs and symptoms. Novel drug delivery system (NDDS), a new approach and has excluded many of drawbacks exhibited by conventional dosage forms. Some of the novel dosage forms of guggul has been formed like nanoparticles, nanovesicles, gugglusomes and proniosomal gel. But still, the novel formulations for guggul has its less outspread in the market. Guggul can be executed as a profitable drug using NDDS. There is a need to highlight the unidentified and unexplained facts about guggul so as to make it more efficacious and effective in terms of bioavailability and aqueous insolubility.

Oleanolic Acid Derived from Plants: Synthesis and Pharmacological Properties of A-ring Modified Derivatives

2020 ◽  
Vol 17 (9) ◽  
pp. 1084-1101
Author(s):  
Tingjuan Wu ◽  
Xu Yao ◽  
Guan Wang ◽  
Xiaohe Liu ◽  
Hongfei Chen ◽  
...  
Keyword(s):  
Drug Design ◽  
Oleanolic Acid ◽  
Water Solubility ◽  
Future Application ◽  
The Novel ◽  
Pharmacological Effects ◽  
Framework Structure ◽  
Design And Synthesis ◽  
The Past ◽  
The Relationship

Background: Oleanolic Acid (OA) is a ubiquitous product of triterpenoid compounds. Due to its inexpensive availability, unique bioactivities, pharmacological effects and non-toxic properties, OA has attracted tremendous interest in the field of drug design and synthesis. Furthermore, many OA derivatives have been developed for ameliorating the poor water solubility and bioavailability. Objective: Over the past few decades, various modifications of the OA framework structure have led to the observation of enhancement in bioactivity. Herein, we focused on the synthesis and medicinal performance of OA derivatives modified on A-ring. Moreover, we clarified the relationship between structures and activities of OA derivatives with different functional groups in A-ring. The future application of OA in the field of drug design and development also was discussed and inferred. Conclusion: This review concluded the novel achievements that could add paramount information to the further study of OA-based drugs.

scholarly journals Novel Tandem Biotransformation Process for the Biosynthesis of a Novel Compound, 4-(2,3,5,6-Tetramethylpyrazine-1)-4′-Demethylepipodophyllotoxin

2011 ◽  
Vol 77 (9) ◽  
pp. 3023-3034 ◽  
Author(s):  
Ya-Jie Tang ◽  
Wei Zhao ◽  
Hong-Mei Li
Keyword(s):  
Cell Line ◽  
Alternaria Alternata ◽  
Water Solubility ◽  
Tumor Cell Line ◽  
Gibberella Fujikuroi ◽  
Hydroxyl Group ◽  
Epithelial Cell Line ◽  
The Novel ◽  
Content Type ◽  
Biotransformation Process

ABSTRACTAccording to the structure of podophyllotoxin and its structure-function relationship, a novel tandem biotransformation process was developed for the directional modification of the podophyllotoxin structure to directionally synthesize a novel compound, 4-(2,3,5,6-tetramethylpyrazine-1)-4′-demethylepipodophyllotoxin (4-TMP-DMEP). In this novel tandem biotransformation process, the starting substrate of podophyllotoxin was biotransformed into 4′-demethylepipodophyllotoxin (product 1) with the demethylation of the methoxyl group at the 4′ position byGibberella fujikuroiSH-f13, which was screened out from Shennongjia prime forest humus soil (Hubei, China). 4′-Demethylepipodophyllotoxin (product 1) was then biotransformed into 4′-demethylpodophyllotoxone (product 2) with the oxidation of the hydroxyl group at the 4 position byAlternaria alternataS-f6, which was screened out from the gatheredDysosma versipellisplants in the Wuhan Botanical Garden, Chinese Academy of Sciences. Finally, 4′-demethylpodophyllotoxone (product 2) and ligustrazine were linked with a transamination reaction to synthesize the target product 4-TMP-DMEP (product 3) byAlternaria alternataS-f6. Compared with podophyllotoxin (i.e., a 50% effective concentration [EC50] of 529 μM), the EC50of 4-TMP-DMEP against the tumor cell line BGC-823 (i.e., 0.11 μM) was significantly reduced by 5,199 times. Simultaneously, the EC50of 4-TMP-DMEP against the normal human proximal tubular epithelial cell line HK-2 (i.e., 0.40 μM) was 66 times higher than that of podophyllotoxin (i.e., 0.006 μM). Furthermore, compared with podophyllotoxin (i.e., logP= 0.34), the water solubility of 4-TMP-DMEP (i.e., logP= 0.66) was significantly enhanced by 94%. For the first time, the novel compound 4-TMP-DMEP with superior antitumor activity was directionally synthesized from podophyllotoxin by the novel tandem biotransformation process developed in this work.

scholarly journals Facile Synthesis of Surface-Modified Carbon Quantum Dots (CQDs) for Biosensing and Bioimaging

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3313 ◽  
Author(s):  
Łukasz Janus ◽  
Julia Radwan-Pragłowska ◽  
Marek Piątkowski ◽  
Dariusz Bogdał
Keyword(s):  
Quantum Dots ◽  
Chemical Structure ◽  
Water Solubility ◽  
Organic Dyes ◽  
Carbon Quantum Dots ◽  
Raw Material ◽  
The Novel ◽  
Surface Modified ◽  
Synthesis Approach ◽  
Effective Analysis

Recently, fluorescent probes became one of the most efficient tools for biosensing and bioimaging. Special attention is focused on carbon quantum dots (CQDs), which are characterized by the water solubility and lack of cytotoxicity. Moreover, they exhibit higher photostability comparing to traditional organic dyes. Currently, there is a great need for the novel, luminescent nanomaterials with tunable properties enabling fast and effective analysis of the biological samples. In this article, we propose a new, ecofriendly bottom-up synthesis approach for intelligent, surface-modified nanodots preparation using bioproducts as a raw material. Obtained nanomaterials were characterized over their morphology, chemical structure and switchable luminescence. Their possible use as a nanodevice for medicine was investigated. Finally, the products were confirmed to be non-toxic to fibroblasts and capable of cell imaging.

scholarly journals Improvement of Imiquimod Solubilization and Skin Retention via TPGS Micelles: Exploiting the Co-Solubilizing Effect of Oleic Acid

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1476
Author(s):  
Martina Ghezzi ◽  
Silvia Pescina ◽  
Andrea Delledonne ◽  
Ilaria Ferraboschi ◽  
Cristina Sissa ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Water Solubility ◽  
Skin Penetration ◽  
Water Soluble ◽  
Drug Solubility ◽  
Two Photon Microscopy ◽  
Skin Delivery ◽  
Polyethylene Glycol 1000 ◽  
The One ◽  
Skin Retention

Imiquimod (IMQ) is an immunostimulant drug approved for the topical treatment of actinic keratosis, external genital-perianal warts as well as superficial basal cell carcinoma that is used off-label for the treatment of different forms of skin cancers, including some malignant melanocytic proliferations such as lentigo maligna, atypical nevi and other in situ melanoma-related diseases. Imiquimod skin delivery has proven to be a real challenge due to its very low water-solubility and reduced skin penetration capacity. The aim of the work was to improve the drug solubility and skin retention using micelles of d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), a water-soluble derivative of vitamin E, co-encapsulating various lipophilic compounds with the potential ability to improve imiquimod affinity for the micellar core, and thus its loading into the nanocarrier. The formulations were characterized in terms of particle size, zeta potential and stability over time and micelles performance on the skin was evaluated through the quantification of imiquimod retention in the skin layers and the visualization of a micelle-loaded fluorescent dye by two-photon microscopy. The results showed that imiquimod solubility strictly depends on the nature and concentration of the co-encapsulated compounds. The micellar formulation based on TPGS and oleic acid was identified as the most interesting in terms of both drug solubility (which was increased from few µg/mL to 1154.01 ± 112.78 µg/mL) and micellar stability (which was evaluated up to 6 months from micelles preparation). The delivery efficiency after the application of this formulation alone or incorporated in hydrogels showed to be 42- and 25-folds higher than the one of the commercial creams.

scholarly journals Possible Arbovirus Found in Virome of Melophagus ovinus

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2375
Author(s):  
Alexander G. Litov ◽  
Oxana A. Belova ◽  
Ivan S. Kholodilov ◽  
Magomed N. Gadzhikurbanov ◽  
Larissa V. Gmyl ◽  
...  
Keyword(s):  
Weight Loss ◽  
Cell Line ◽  
Mammalian Cells ◽  
Skin Irritation ◽  
The Novel ◽  
Skin Injuries ◽  
Blood Sucking ◽  
Melophagus Ovinus ◽  
Porcine Embryo ◽  
The Republic

Members of the Lipopteninae subfamily are blood-sucking ectoparasites of mammals. The sheep ked (Melophagus ovinus) is a widely distributed ectoparasite of sheep. It can be found in most sheep-rearing areas and can cause skin irritation, restlessness, anemia, weight loss and skin injuries. Various bacteria and some viruses have been detected in M. ovinus; however, the virome of this ked has never been studied using modern approaches. Here, we study the virome of M. ovinus collected in the Republic of Tuva, Russia. In our research, we were able to assemble full genomes for five novel viruses, related to the Rhabdoviridae (Sigmavirus), Iflaviridae, Reoviridae and Solemoviridae families. Four viruses were found in all five of the studied pools, while one virus was found in two pools. Phylogenetically, all of the novel viruses clustered together with various recently described arthropod viruses. All the discovered viruses were tested on their ability to replicate in the mammalian porcine embryo kidney (PEK) cell line. Aksy-Durug Melophagus sigmavirus RNA was detected in the PEK cell line cultural supernate after the first, second and third passages. Such data imply that this virus might be able to replicate in mammalian cells, and thus, can be considered as a possible arbovirus.

scholarly journals Transforaminal Epidural Balloon Adhesiolysis via a Contralateral Interlaminar Retrograde Foraminal Approach: A Retrospective Analysis and Technical Considerations

2020 ◽  
Vol 9 (4) ◽  
pp. 981
Author(s):  
Chan-Sik Kim ◽  
Yeon-Jin Moon ◽  
Jae Won Kim ◽  
Dong-Min Hyun ◽  
Shill Lee Son ◽  
...  
Keyword(s):  
Iliac Crest ◽  
Treatment Options ◽  
Balloon Catheter ◽  
Radicular Pain ◽  
Treatment Modalities ◽  
Foraminal Stenosis ◽  
The Novel ◽  
Lumbar Foraminal Stenosis ◽  
Transforaminal Approach ◽  
Technical Considerations

Several treatment modalities have been proposed for foraminal stenosis, but the treatment options remain unsatisfactory. Previous studies have shown that transforaminal balloon adhesiolysis may be effective in patients with refractory lumbar foraminal stenosis. However, in patients with a high iliac crest, balloon catheter insertion may be difficult via a conventional transforaminal approach (particularly targeting the L5–S1 foramen). It has been reported that an epidural catheter can be placed easily by a contralateral interlaminar retrograde foraminal approach. Therefore, we applied this approach to L5–S1 transforaminal balloon adhesiolysis in patients with a high iliac crest. We retrospectively analyzed data from 22 patients who underwent combined epidural adhesiolysis and balloon decompression (balloon adhesiolysis) using the novel foraminal balloon catheter via a contralateral interlaminar retrograde foraminal approach. The pain intensity significantly decreased over the three-month period after balloon adhesiolysis (p < 0.001). There were no complications associated with the balloon procedure. The present study suggests that balloon adhesiolysis for L5-S1 foramen via a contralateral interlaminar retrograde foraminal approach may be an effective alternative for patients with a high iliac crest and refractory lumbar radicular pain due to lumbar foraminal stenosis. In addition, detailed procedural aspects are described here.

scholarly journals A Study of the Disruptive Effect of the Acetate Fraction of Punica granatum Extract on Cryptococcus Biofilms

2021 ◽  
Vol 11 ◽  
Author(s):  
Paulo C. M. Villis ◽  
Alessandra T. de Macedo ◽  
Haryne L. A. Furtado ◽  
Pedro H. C. Fontenelle ◽  
Ingrid S. Gonçalves ◽  
...  
Keyword(s):  
Treatment Options ◽  
Folk Medicine ◽  
Antimicrobial Effect ◽  
Punica Granatum ◽  
Cryptococcus Laurentii ◽  
Disruptive Effect ◽  
Worldwide Distribution ◽  
Kill Curve ◽  
Therapeutic Alternatives ◽  
Time Kill

Cryptococcosis, caused by yeasts of the genus Cryptococcus, is an infectious disease with a worldwide distribution. Cryptococcus neoformans and Cryptococcus gattii are the species that commonly cause this disease in humans; however, infections caused by Cryptococcus laurentii, especially in immunocompromised patients, are increasingly being reported. Owing to the increase in the resistance of fungi to antifungals, and a lack of treatment options, it is important to seek new therapeutic alternatives such as natural products. Among these are plant species such as Punica granatum, which is used in folk medicine to treat various diseases. This study aimed to evaluate the activity of the acetate fraction of P. granatum leaf extract against environmental and clinical isolates of Cryptococcus. Three environmental isolates of C. laurentii, PMN, PMA, and PJL II, isolated from soils of different municipalities in the state of Maranhão, a clinical isolate, C. gattii, from a patient with neurocryptococcosis, and a standard strain of C. gattii (ATCC 32068) were used. The minimum and fractional inhibitory concentrations (MIC and FIC, respectively) and time-kill curve of the extract and fluconazole were determined to assess the susceptibility profile of the fungal isolates. Larvae of Tenebrio molitor were infected with Cryptococcus strains, and the effects of acetate fraction of P. granatum extract and fluconazole on the survival and fungal burden were determined. The extract activity was tested against pre-formed biofilms. The acetate fraction of P. granatum extract showed promising antifungal activity against all the species of Cryptococcus evaluated in this study, with an MIC value lower than that of fluconazole. The indices obtained in the FIC test indicated that the antimicrobial effect of the combination of the extract and antifungal was indifferent for 80% of the isolates. The P. granatum acetate fraction reduced the pre-formed biofilm of some isolates, showing better activity than fluconazole, which is consistent with results from fluorescence microscopy. This is the first study on the use of P. granatum and its ability to inhibit Cryptococcus biofilms; therefore, further studies and tests are needed to investigate the components and mechanism of action of P. granatum against cryptococcosis agents.

scholarly journals Imagenological findings of external snapping hip syndrome. Case report

Case reports ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 123-131
Author(s):  
Ingrid Carolina Donoso-Donoso ◽  
Enrique Calvo-Páramo ◽  
Roger David Medina-Ramírez
Keyword(s):  
Treatment Options ◽  
Multidisciplinary Treatment ◽  
Iliotibial Band ◽  
Snapping Hip ◽  
Adequate Response ◽  
Painful Sensation ◽  
External Snapping Hip ◽  
The Right ◽  
Snapping Hip Syndrome ◽  
Timely Treatment

Introduction: External snapping hip syndrome is characterized by a painful sensation accom­panied by an audible snapping noise in the hip when moving. Even though orthopedists are widely aware of this condition, imaging findings still need to be recognized by all radiologists in order to provide more information that allows for the best multidisciplinary treatment. Z-plasty of the iliotibial band is the most used treatment with the best results.Case presentation: Female patient with bi­lateral external hip snapping syndrome on the right side, who was treated initially in a con­servative manner without adequate response; hence, she required surgical management with arthroscopy. All treatment options used for this patient were not successful, and symptoms recurred.Discussion: The diagnosis of snapping hip syndrome is mainly clinical. However, the con­tribution of diagnostic imaging is important to characterize the structures involved in this nosological process, in order to develop the therapeutic planning and do the follow-up.Conclusion: Knowledge on ultrasound and magnetic resonance findings related to this pathology allows radiologists to identify this syndrome and contribute to a timely treatment.

Ultrasound-guided TriVex™-powered phlebectomy for debulking of peripheral vascular anomalies – A novel treatment technique

2019 ◽  
Vol 34 (8) ◽  
pp. 523-529
Author(s):  
Kurosh Parsi ◽  
Mina Kang ◽  
Anthony Trimboli
Keyword(s):  
Treatment Options ◽  
Symptom Relief ◽  
Surgical Excision ◽  
Target Lesion ◽  
Vascular Anomalies ◽  
Treatment Technique ◽  
The Novel ◽  
Ultrasound Guided ◽  
Surgical Debulking ◽  
Fibrofatty Tissue

Background Large vascular anomalies on the trunk or limbs are rare and prove challenging to treat. Currently, treatment options include conservative management, embolisation, sclerotherapy or surgical excision. Once the lesion has been embolised, or if it spontaneously involutes, the residual fibrofatty tissue may be painful and require debulking. Surgical debulking may be associated with complicated wound healing, infection, scarring and may not be not feasible for large lesions. Method We present our technique of using TriVex™-powered phlebectomy to debulk vascular anomalies. Our modification involved performing sclero-emobolisation on the target lesion first to mitigate the risk of haematoma formation. We also used ultrasound guidance to increase the precision and eliminate the use of TriVex™ illuminator. Results Excellent symptom relief and cosmetic outcome were achieved in three patients treated using this method. Conclusion We believe the novel technique of ultrasound-guided TriVex™-powered system provides a viable alternative to surgical debulking of large or complex vascular anomalies.

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