scholarly journals Bioactivity-Guided Purification of Novel Herbal Antioxidant and Anti-NO Compounds from Euonymus laxiflorus Champ.

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 120 ◽  
Author(s):  
Van Nguyen ◽  
San-Lang Wang ◽  
Anh Nguyen ◽  
Zhi-Hu Lin ◽  
Chien Doan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Biological Activities ◽  
The Novel ◽  
Health Food ◽  
Ic50 Values ◽  
Comparable Activity ◽  
New Compounds ◽  
First Time ◽  
Antidiabetic Effects

Euonymus laxiflorus Champ., a medicinal herb collected in Vietnam, has been reported to show several potent bioactivities, including anti-NO, enzyme inhibition, hypoglycemic and antidiabetic effects. Recently, the antioxidant activity of Euonymus laxiflorus Champ. trunk bark (ELCTB) has also been reported. However, the active antioxidant and anti-NO constituents existing in ELCTB have not been reported in the literature. The objective of this study was to purify the active antioxidants from ELCTB and investigate the anti-NO effect of the major constituents. Twenty-two phenolics isolated from ELCTB, including 12 compounds newly isolated in this study (1–12) and 10 constituents obtained from our previous work, were evaluated for their antioxidant activity. Of these, 12 compounds (4–6, 9, 13–15, 18–22) showed a potent antioxidant capacity (FRS50 = 7.8–58.11 µg/mL), in comparison to α-tocopherol (FRS50 = 23 µg/mL). In the anti-NO activity tests, Walterolactone A (1a) and B (1b) β-d-glucopyranoside (13) demonstrated the most effective and comparable activity to that of quercetin with max inhibition and IC50 values of 100%, 1.3 µg/mL, and 100%, 1.21 µg/mL, respectively. The crude extract and its major compounds showed no cytotoxicity on normal cells. Notably, three constituents (9, 11, and 12) were identified as new compounds, another three constituents, including 1, 7, and 8, were found to be new natural products, constituents 9 and 13 were determined to be new antioxidants, and compound 13 was reported to have novel potent anti-NO activity for the first time. The results of this study contribute to the enrichment of new natural products and compounds, as well as the novel biological activity of constituents isolated from Euonymus laxiflorus Champ. The current study also indicates ELCTB as a rich natural source of active phenolics. It is suggested that ELCTB could be developed as a health food with promising antioxidant and anti-NO effects, as well as other beneficial biological activities.

scholarly journals Biological Activities of Natural Products

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5769
Author(s):  
Halina Maria Ekiert ◽  
Agnieszka Szopa
Keyword(s):  
Natural Products ◽  
Biological Activities ◽  
Health Food

Natural products and their biological activities are currently a subject of great interest in the pharmaceutical, health food, and cosmetics industries, and numbers of scientific studies in this field are increasing rapidly [...]

scholarly journals Terpenoids from the Deep-Sea-Derived Fungus Penicillium thomii YPGA3 and Their Bioactivities

Marine Drugs ◽  
2020 ◽  
Vol 18 (3) ◽  
pp. 164 ◽  
Author(s):  
Zhongbin Cheng ◽  
Wan Liu ◽  
Runzhu Fan ◽  
Shouye Han ◽  
Yuanli Li ◽  
...  
Keyword(s):  
Deep Sea ◽  
Chemical Study ◽  
Positive Control ◽  
Hydroxyl Group ◽  
Etoac Extract ◽  
The Third ◽  
Ic50 Value ◽  
Ic50 Values ◽  
New Compounds ◽  
First Time

A chemical study of the ethyl acetate (EtOAc) extract from the deep-sea-derived fungus Penicillium thomii YPGA3 led to the isolation of a new austalide meroterpenoid (1) and seven known analogues (2−8), two new labdane-type diterpenoids (9 and 10) and a known derivative (11). The structures of new compounds 1, 9, and 10 were determined by comprehensive analyses via nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data. The absolute configurations of 1, 9, and 10 were determined by comparisons of experimental electronic circular dichroism (ECD) with the calculated ECD spectra. Compound 1 represented the third example of austalides bearing a hydroxyl group at C-5 instead of the conserved methoxy in other known analogues. To our knowledge, diterpenoids belonging to the labdane-type were discovered from species of Penicillium for the first time. Compound 1 showed cytotoxicity toward MDA-MB-468 cells with an IC50 value of 38.9 μM. Compounds 2 and 11 exhibited inhibition against α-glucosidase with IC50 values of 910 and 525 μM, respectively, being more active than the positive control acarbose (1.33 mM).

scholarly journals Asperienes A–D, Bioactive Sesquiterpenes from the Marine-Derived Fungus Aspergillus flavus

Marine Drugs ◽  
2019 ◽  
Vol 17 (10) ◽  
pp. 550 ◽  
Author(s):  
Yun-Feng Liu ◽  
Yu-Fei Yue ◽  
Li-Xi Feng ◽  
Hua-Jie Zhu ◽  
Fei Cao
Keyword(s):  
Cell Lines ◽  
Aspergillus Flavus ◽  
Solid Medium ◽  
Biological Activities ◽  
Antitumor Agent ◽  
Minor Components ◽  
Electronic Circular Dichroism ◽  
The Future ◽  
Ic50 Values ◽  
First Time

Marine-derived fungi of the genera Aspergillus could produce novel compounds with significant bioactivities. Among these fungi, the strain Aspergillus flavus is notorious for its mutagenic mycotoxins production. However, some minor components with certain toxicities from A. flavus have not been specifically surveyed and might have potent biological activities. Our investigation of the marine-derived fungus Aspergillus flavus CF13-11 cultured in solid medium led to the isolation of four C-6′/C-7′ epimeric drimane sesquiterpene esters, asperienes A–D (1–4). Their absolute configurations were assigned by electronic circular dichroism (ECD) and Snatzke’s methods. This is the first time that two pairs of C-6′/C-7′ epimeric drimane sesquiterpene esters have successfully been separated. Aperienes A–D (1–4) displayed potent bioactivities towards four cell lines with the IC50 values ranging from 1.4 to 8.3 μM. Interestingly, compounds 1 and 4 exhibited lower toxicities than 2 and 3 toward normal GES-1 cells, indicating more potential for development as an antitumor agent in the future.

scholarly journals A First Approach to the Chemical Composition and Antioxidant Potential of Guinea-Bissau Propolis

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984413 ◽  
Author(s):  
Soraia I. Falcão ◽  
Mélissa Lopes ◽  
Miguel Vilas-Boas
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Physicochemical Properties ◽  
Natural Product ◽  
Broad Spectrum ◽  
Biological Activities ◽  
Chemical Diversity ◽  
Antioxidant Potential ◽  
Phenolic Composition ◽  
First Time

Propolis is a natural product derived from plant resins collected by honeybees and used in the beehive as a construction and defensive material. The broad spectrum of biological activities is dependent on the chemical diversity of its composition which is determined by the floral sources at the site of collection. For the first time, the chemical composition of Guinean propolis as well as its physicochemical properties, phenolic composition, and antioxidant activity was assessed. Eight compounds were identified through LC/DAD/ESI-MS n , mostly isoflavonoids, resembling Nigerian and Brazilian red propolis from the genus Dalbergia.

scholarly journals Chemical Constituents and Antioxidant and Biological Activities of the Essential Oil from Leaves of Solanum spirale

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Sukanya Keawsa-ard ◽  
Boonsom Liawruangrath ◽  
Saisunee Liawruangrath ◽  
Aphiwat Teerawutgulrag ◽  
Stephen G. Pyne
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Octadecanoic Acid ◽  
Small Cell Lung ◽  
Hexahydrofarnesyl Acetone ◽  
First Time ◽  
Mcf 7

The essential oil of the leaves Solanum spirale Roxb. was isolated by hydrodistillation and analyzed for the first time using GC and GC-MS. Thirty-nine constituents were identified, constituting 73.36% of the total chromatographical oil components. ( E)-Phytol (48.10%), n-hexadecanoic acid (7.34%), β-selinene (3.67%), α-selinene (2.74%), octadecanoic acid (2.12%) and hexahydrofarnesyl acetone (2.00%) were the major components of this oil. The antioxidant activity of the essential oil was evaluated by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. The oil exhibited week antioxidant activity with an IC50 of 41.89 mg/mL. The essential oil showed significant antibacterial activity against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus with MIC values of 43.0 μg/mL and 21.5 μg/mL, respectively. It also showed significant cytotoxicity against KB (oral cancer), MCF-7 (breast cancer) and NCI-H187 (small cell lung cancer) with the IC50 values of 26.42, 19.69, and 24.02 μg/mL, respectively.

Isolation and Biological Activities of Citral from Sweet Orange Oil

2013 ◽  
Vol 750-752 ◽  
pp. 1621-1625 ◽  
Author(s):  
Xiao Lin Xu ◽  
Qing Zhu ◽  
Hui Lv ◽  
Wen Hui Wu ◽  
Min Lv ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sweet Orange ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Cell Line ◽  
Orange Oil ◽  
Cell Line Hela ◽  
Ic50 Values

The purpose of this study was to isolate citral from sweet orange oil by combined usage of molecular distillation and column chromatography. Additionally, the antioxidant activity (by DPPH radical scavenging activity and reducing power) of citral was evaluated. A significant and linear correlation coefficient between the antioxidant activity and the concentration of citral was found (R2=0.9925). On the other hand, the cytotoxic effects of the citral against tumoral human cell line Hela was examined by MTT assay and the IC50 values was 32.56 μg/mL. Overall, results presented here suggest that the citral possesses antioxidant activity and cytotoxic properties, and is a potential source of active ingredients for food and pharmaceutical industry.

scholarly journals Enriching the Arsenal of Pharmacological Tools against MICAL2

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7519
Author(s):  
Ivana Barravecchia ◽  
Elisabetta Barresi ◽  
Camilla Russo ◽  
Francesca Scebba ◽  
Chiara De Cesari ◽  
...  
Keyword(s):  
Human Cancer ◽  
Biological Activities ◽  
Microvascular Endothelial Cells ◽  
The Novel ◽  
Invasive Front ◽  
Structure Activity ◽  
Molecule Inhibitor ◽  
Cancer Types ◽  
Cytoskeleton Dynamics ◽  
New Compounds

Molecule interacting with CasL 2 (MICAL2), a cytoskeleton dynamics regulator, are strongly expressed in several human cancer types, especially at the invasive front, in metastasizing cancer cells and in the neo-angiogenic vasculature. Although a plethora of data exist and stress a growing relevance of MICAL2 to human cancer, it is worth noting that only one small-molecule inhibitor, named CCG-1423 (1), is known to date. Herein, with the aim to develop novel MICAL2 inhibitors, starting from CCG-1423 (1), a small library of new compounds was synthetized and biologically evaluated on human dermal microvascular endothelial cells (HMEC-1) and on renal cell adenocarcinoma (786-O) cells. Among the novel compounds, 10 and 7 gave interesting results in terms of reduction in cell proliferation and/or motility, whereas no effects were observed in MICAL2-knocked down cells. Aside from the interesting biological activities, this work provides the first structure–activity relationships (SARs) of CCG-1423 (1), thus providing precious information for the discovery of new MICAL2 inhibitors.

scholarly journals Phytochemistry and Antioxidant Activities of the Methanolic Leaf Extract of Clerodendrum splendens (Lamiaceae)

2018 ◽  
pp. 12-20
Author(s):  
Nganso Ditchou Yves Oscar ◽  
Amang A Ngoung GA ◽  
Soh Desire ◽  
Simo Nemg Fredy Brice ◽  
Nyasse Barthelemy
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Maximum Activity ◽  
Hepatic Lipids ◽  
Ic50 Value ◽  
Ic50 Values

This paper aimed at studying the antioxidant efficacy of the methanolic leaf extract of Clerodendrum splendens, a plant of the Lamiaceae family. Phytochemical tests carried out on extracts of Clerodendrum splendens leaves have been able to detect the presence of secondary metabolites such as Flavonoids, Tannins, Saponins and Terpenoids. The results of the antioxidant activity have shown that CSF2, CSF3 fractions and CSB, CSG fractions similarly inhibited hepatic lipids but significantly less than vitamin C. Compared to all fractions, the CSB fraction shows the best inhibitor on the peroxidation of hepatic lipids because at 150 μg/mL, there is a maximum activity (2.5 μg/mL of protein). However, it is found that CSF3, CSF2 and CSG have higher IC50 values than vitamin C (5.613±0.117) while CSEB, CSB and CSC fractions showed lower IC50 values than vitamin C, which is used as the reference reducing compound. The lower the IC50 value compared to vitamin C, the greater the antioxidant capacity of the plant extract. The results of this study suggest that Clerodendrum splendens represents an untapped source of compounds with potential antioxidant activity that could be explored in the development of new therapeutic natural products.

scholarly journals Phytochemicals, Antimicrobials and Antioxidants Studies of the Stem Bark Extract from Calophyllum ferrugineum

2020 ◽  
Vol 17 (2) ◽  
pp. 1
Author(s):  
Izzah Afifah Noh ◽  
Vivien Yi Mian Jong
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Biological Activities ◽  
Stem Bark ◽  
Extraction Process ◽  
Bark Extract ◽  
Chemical Components ◽  
Isolation And Purification ◽  
Antioxidant Agents ◽  
Ic50 Values

Traditionally, Calophyllum genus was used for swollen gums, arthritis, diarrhea, chronic abscess, skin infections, and lesions treatment. C. ferrugineum from Sarawak has least studied about their phytochemical constituents and biological activities. The objectives are to isolate and characterize the chemical components from C. ferrugineum and to determine their antimicrobial and antioxidant activity. The plant stem barks were collected from National Park in Sarawak and underwent extraction process. The extracts underwent isolation and purification processes by using several chromatographic methods. Structural elucidation was achieved by using infrared, MS and NMR spectra. The isolation process from the species has afforded 5 known compounds;  isocalanone (1) and 1-hydroxy-7-methoxy-9H-xanthen-9-one (2), lupeol (3), friedelin (4) and diethylene glycol dibenzoate (5). The chloroform and methanol extract showed strong inhibitions against S. aureus, B. subtilis, P. aeruginosa and E. coli with the MIC and MBC values ranging from 225 and 112.5 µg/mL. Isocalanone (1) and the methanol extract of C. ferrugineum showed potent antioxidant activity with the IC50 values of 28 ± 5.23 and 35 ± 3.69 µg/mL compared to ascorbic acid, respectively. The results obtained from this study emphasized the potential of the species as antimicrobial and antioxidant agents.

scholarly journals Habitat Affects the Chemical Profile, Allelopathy, and Antioxidant Properties of Essential Oils and Phenolic Enriched Extracts of the Invasive Plant Heliotropium Curassavicum

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Invasive Plant ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Bioactive Constituents ◽  
Chenopodium Murale ◽  
Two Samples ◽  
Ic50 Values

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.

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