scholarly journals Faster Removal of 2-Phosphoglycolate through Photorespiration Improves Abiotic Stress Tolerance of Arabidopsis

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 563 ◽  
Author(s):  
Stefan Timm ◽  
Franziska Woitschach ◽  
Carolin Heise ◽  
Martin Hagemann ◽  
Hermann Bauwe
Keyword(s):  
Abiotic Stress ◽  
Elevated Temperature ◽  
Elevated Temperatures ◽  
Soluble Sugar ◽  
Carbon Assimilation ◽  
High Light ◽  
Starch Accumulation ◽  
Wild Type ◽  
Transgenic Lines ◽  
The Impact

Photorespiration metabolizes 2-phosphoglyolate (2-PG) to avoid inhibition of carbon assimilation and allocation. In addition to 2-PG removal, photorespiration has been shown to play a role in stress protection. Here, we studied the impact of faster 2-PG degradation through overexpression of 2-PG phosphatase (PGLP) on the abiotic stress-response of Arabidopsis thaliana (Arabidopsis). Two transgenic lines and the wild type were subjected to short-time high light and elevated temperature stress during gas exchange measurements. Furthermore, the same lines were exposed to long-term water shortage and elevated temperature stresses. Faster 2-PG degradation allowed maintenance of photosynthesis at combined light and temperatures stress and under water-limiting conditions. The PGLP-overexpressing lines also showed higher photosynthesis compared to the wild type if grown in high temperatures, which also led to increased starch accumulation and shifts in soluble sugar contents. However, only minor effects were detected on amino and organic acid levels. The wild type responded to elevated temperatures with elevated mRNA and protein levels of photorespiratory enzymes, while the transgenic lines displayed only minor changes. Collectively, these results strengthen our previous hypothesis that a faster photorespiratory metabolism improves tolerance against unfavorable environmental conditions, such as high light intensity and temperature as well as drought. In case of PGLP, the likely mechanism is alleviation of inhibitory feedback of 2-PG onto the Calvin–Benson cycle, facilitating carbon assimilation and accumulation of transitory starch.

scholarly journals R-BPMV-Mediated Resistance to Bean pod mottle virus in Phaseolus vulgaris L. Is Heat-Stable but Elevated Temperatures Boost Viral Infection in Susceptible Genotypes

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1239
Author(s):  
Chouaïb Meziadi ◽  
Julie Lintz ◽  
Masoud Naderpour ◽  
Charlotte Gautier ◽  
Sophie Blanchet ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Phaseolus Vulgaris ◽  
Elevated Temperatures ◽  
Heat Waves ◽  
Dominant Gene ◽  
Atmospheric Temperature ◽  
Mottle Virus ◽  
Bean Pod Mottle Virus ◽  
Heat Stable ◽  
The Impact

In the context of climate change, elevated temperature is a major concern due to the impact on plant–pathogen interactions. Although atmospheric temperature is predicted to increase in the next century, heat waves during summer seasons have already become a current problem. Elevated temperatures strongly influence plant–virus interactions, the most drastic effect being a breakdown of plant viral resistance conferred by some major resistance genes. In this work, we focused on the R-BPMV gene, a major resistance gene against Bean pod mottle virus in Phaseolus vulgaris. We inoculated different BPMV constructs in order to study the behavior of the R-BPMV-mediated resistance at normal (20 °C) and elevated temperatures (constant 25, 30, and 35 °C). Our results show that R-BPMV mediates a temperature-dependent phenotype of resistance from hypersensitive reaction at 20 °C to chlorotic lesions at 35 °C in the resistant genotype BAT93. BPMV is detected in inoculated leaves but not in systemic ones, suggesting that the resistance remains heat-stable up to 35 °C. R-BPMV segregates as an incompletely dominant gene in an F2 population. We also investigated the impact of elevated temperature on BPMV infection in susceptible genotypes, and our results reveal that elevated temperatures boost BPMV infection both locally and systemically in susceptible genotypes.

scholarly journals Experiment on Foreign Object Damage of Gas Turbine-Grade Silicon Nitride Ceramic

1998 ◽  
Author(s):  
Hiro Yoshida ◽  
Takashi Nakashima ◽  
Makoto Yoshida ◽  
Yasushi Hara ◽  
Toru Shimamori
Keyword(s):  
Elevated Temperature ◽  
Silicon Nitride ◽  
Elevated Temperatures ◽  
Tensile Load ◽  
Strength Degradation ◽  
Spall Fracture ◽  
Silicon Nitride Ceramic ◽  
Impact Tests ◽  
The Impact ◽  
Bending Fracture

A new high quality turbine system using monolithic silicon-nitride ceramic is under development. In this study particle impact tests of the silicon-nitride have been tried at room and elevated temperatures with and without tensile load, which simulates centrifugal force of blade rotation. In the experiment 1 mm diameter particle is impacted at velocities up to 900 m s−1. In this paper, critical velocities for bending fracture and Hertzian cracks are examined. Moreover, strength degradation at elevated temperature and spall fracture of the blade are discussed. The main results are: 1) The bending fracture mode critical impact velocity for soft particles is higher than that for hard particles. 2)The impact parameter ϕ for initiation of Hertzian cracks ranges 1.08×10−5 – 1.56×10−5 for the materials tested. 3)Strength degradation at elevated temperature was clearly observed. 4) In the impact tests on blades spall fracture, which was caused by interaction of stress waves, appeared.

scholarly journals Study on Tensile Properties of CFRP Plates under Elevated Temperature Exposure

Materials ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 1995 ◽  
Author(s):  
Yongxin Yang ◽  
Yanju Jiang ◽  
Hongjun Liang ◽  
Xiaosan Yin ◽  
Yue Huang
Keyword(s):  
Tensile Strength ◽  
Elastic Modulus ◽  
Elevated Temperature ◽  
Tensile Properties ◽  
Failure Mode ◽  
Elevated Temperatures ◽  
Strain Curve ◽  
Effect Of Temperature ◽  
Temperature Exposure ◽  
The Impact

Elevated temperature exposure has a negative effect on the performance of the matrix resin in Carbon Fiber Reinforced Plastics (CFRP) plates, whereas limited quantitative research focuses on the deteriorations. Therefore, 30 CFRP specimens were designed and tested under elevated temperatures (10, 30, 50, 70, and 90 °C) to explore the degradations in tensile properties. The effect of temperature on the failure mode, stress-strain curve, tensile strength, elastic modulus and elongation of CFRP plates were investigated. The results showed that elevated temperature exposure significantly changed the failure characteristics. When the exposed temperature increased from 10 °C to 90 °C, the failure mode changed from the global factures in the whole CFRP plate to the successive fractures in carbon fibers. Moreover, with temperatures increasing, tensile strength and elongation of CFRP plates decreases gradually while the elastic modulus shows negligible change. Finally, the results of One-Way Analysis of Variance (ANOVA) show that the degradation of the tensile strength of CFRP plates was due to the impact of elevated temperature exposure, rather than the test error.

scholarly journals Knockout of SlSBPASE Suppresses Carbon Assimilation and Alters Nitrogen Metabolism in Tomato Plants

2018 ◽  
Vol 19 (12) ◽  
pp. 4046 ◽  
Author(s):  
Fei Ding ◽  
Qiannan Hu ◽  
Meiling Wang ◽  
Shuoxin Zhang
Keyword(s):  
Nitrogen Metabolism ◽  
Substantial Reduction ◽  
Carbon Assimilation ◽  
Optimal Growth ◽  
Wild Type ◽  
Tomato Plants ◽  
Carbon Assimilation Rate ◽  
The Impact ◽  
Severe Growth

Sedoheptulose-1,7-bisphosphatase (SBPase) is an enzyme in the Calvin–Benson cycle and has been documented to be important in carbon assimilation, growth and stress tolerance in plants. However, information on the impact of SBPase on carbon assimilation and nitrogen metabolism in tomato plants (Solanum lycopersicum) is rather limited. In the present study, we investigated the role of SBPase in carbon assimilation and nitrogen metabolism in tomato plants by knocking out SBPase gene SlSBPASE using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene editing technology. Compared with wild-type plants, slsbpase mutant plants displayed severe growth retardation. Further analyses showed that knockout of SlSBPASE led to a substantial reduction in SBPase activity and as a consequence, ribulose-1,5-bisphosphate (RuBP) regeneration and carbon assimilation rate were dramatically inhibited in slsbpase mutant plants. It was further observed that much lower levels of sucrose and starch were accumulated in slsbpase mutant plants than their wild-type counterparts during the photoperiod. Intriguingly, mutation in SlSBPASE altered nitrogen metabolism as demonstrated by changes in levels of protein and amino acids and activities of nitrogen metabolic enzymes. Collectively, our data suggest that SlSBPASE is required for optimal growth, carbon assimilation and nitrogen metabolism in tomato plants.

scholarly journals The Impact of Interphase Precipitation on the Mechanical Behavior of Fire-Resistant Steels at an Elevated Temperature

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4294
Author(s):  
Jinghua Cong ◽  
Jiangwen Li ◽  
Jiajie Fan ◽  
Pengcheng Liu ◽  
Raja Devesh Kumar Misra ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Elevated Temperatures ◽  
Volume Fraction ◽  
Isothermal Holding ◽  
High Strength ◽  
Controlled Rolling ◽  
Thermally Stable ◽  
Laminar Cooling ◽  
Interphase Precipitation ◽  
The Impact

In this study, we address the challenge of obtaining high strength at ambient and elevated temperatures in fire-resistant Ti–Mo–V steel with ferrite microstructures through thermo-mechanical controlled processing (TMCP). Thermally stable interphase precipitation of (Ti, Mo, V)C was an important criterion for retaining strength at elevated temperatures. Electron microscopy indicated that interphase precipitation occurred during continuous cooling after controlled rolling, where the volume fraction of interphase precipitation was controlled by the laminar cooling temperature. The interphase precipitation of MC carbides with an NaCl-type crystal structure indicated a Baker–Nutting (B–N) orientation relationship with ferrite. When the steel was isothermally held at 600 °C for up to 3 h, interphase precipitation occurred during TMCP with high thermal stability. At the same time, some random precipitation took place during isothermal holding. The interphase precipitation increased the elastic modulus of the experimental steels at an elevated temperature. It is proposed that fire-resistant steel with thermally stable interphase precipitation is preferred, which enhances precipitation strengthening and dislocation strengthening at elevated temperatures.

Isothermal Aging Effects on the Thermal reliability Performance of Lead-Free Solder Joints

2012 ◽  
Vol 2012 (1) ◽  
pp. 000801-000808 ◽  
Author(s):  
Jiawei Zhang ◽  
Zhou Hai ◽  
Sivasubramanian Thirugnanasambandam ◽  
John L. Evans ◽  
M. J. Bozack
Keyword(s):  
Elevated Temperature ◽  
Isothermal Aging ◽  
Elevated Temperatures ◽  
Solder Joints ◽  
Bulk Solder ◽  
Lead Free ◽  
Aging Effects ◽  
Thermal Reliability ◽  
Free Solder ◽  
The Impact

Electronics assemblies containing solder joints are often exposed to elevated temperatures for prolonged periods of time. The time-at-temperature stress impacts the overall package reliability of the assembled circuitry due to evolving materials, microstructural, and mechanical properties. It is especially important to understand the impact of isothermal aging on the long term behavior of lead (Pb)-free solder joints which operate in harsh environments. In this study, we have explored the effects of elevated temperature isothermal aging on the reliability of Sn-Ag-Cu (SAC) assemblies on board level packages. As the isothermal aging temperature increases, the Weybull characteristic lifetime for SAC 105 and 305 solder joints is drastically reduced compared to Sn-37Pb.In parallel mechanical studies on bulk solder specimens, the creep rate for SAC105, 305 rapidly increases with aging. A full test matrix with varying aging temperatures and solder alloys was considered. Package sizes ranged from 19mm, 0.8mm pitch ball grid arrays (BGAs) to 5mm, 0.4mm pitch μBGAs. The test structures were built on three different board finishes (ImSn, ImAg and SnPb). Storage condition temperatures were 25°C, 55°C, 85°C and 125°C with aging over time periods of 0, 6, and 12 months. Subsequently, the specimens were thermally cycled from −40°C to 125°C with 15 min dwell times at the high temperature. It was found that the thermal performance of lead-free fine-pitch packages significantly degrades up to 55–60% after aging at elevated temperature. The dominant failure mode can be associated with the growth of Cu6Sn5 intermetallic compounds (IMC) during the aging, particularly on the pad side.

scholarly journals Chrysanthemum DgWRKY2 Gene Enhances Tolerance to Salt Stress in Transgenic Chrysanthemum

2018 ◽  
Vol 19 (7) ◽  
pp. 2062 ◽  
Author(s):  
Ling He ◽  
Yin-Huan Wu ◽  
Qian Zhao ◽  
Bei Wang ◽  
Qing-Lin Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Wild Type ◽  
Acid Protein ◽  
Transgenic Lines ◽  
High Salt Stress ◽  
Transgenic Chrysanthemum ◽  
Stress Related Genes ◽  
Amino Acid Protein

WRKY transcription factors (TFs) play a vital part in coping with different stresses. In this study, DgWRKY2 was isolated from Dendranthema grandiflorum. The gene encodes a 325 amino acid protein, belonging to the group II WRKY family, and contains one typical WRKY domain (WRKYGQK) and a zinc finger motif (C-X4-5-C-X22-23-H-X1-H). Overexpression of DgWRKY2 in chrysanthemum enhanced tolerance to high-salt stress compared to the wild type (WT). In addition, the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)), proline content, soluble sugar content, soluble protein content, and chlorophyll content of transgenic chrysanthemum, as well as the survival rate of the transgenic lines, were on average higher than that of the WT. On the contrary, hydrogen peroxide (H2O2), superoxide anion (O2−), and malondialdehyde (MDA) accumulation decreased compared to WT. Expression of the stress-related genes DgCAT, DgAPX, DgZnSOD, DgP5CS, DgDREB1A, and DgDREB2A was increased in the DgWRKY2 transgenic chrysanthemum compared with their expression in the WT. In conclusion, our results indicate that DgWRKY2 confers salt tolerance to transgenic chrysanthemum by enhancing antioxidant and osmotic adjustment. Therefore, this study suggests that DgWRKY2 could be used as a reserve gene for salt-tolerant plant breeding.

scholarly journals Involvement of Lhcb6 and Lhcb5 in Photosynthesis Regulation in Physcomitrella patens Response to Abiotic Stress

2019 ◽  
Vol 20 (15) ◽  
pp. 3665 ◽  
Author(s):  
Xingji Peng ◽  
Xingguang Deng ◽  
Xiaoya Tang ◽  
Tinghong Tan ◽  
Dawei Zhang ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Physcomitrella Patens ◽  
Light Harvesting ◽  
Light Stress ◽  
High Light ◽  
Photochemical Quenching ◽  
Wild Type ◽  
Knock Out ◽  
Non Photochemical Quenching ◽  
Photosynthesis Regulation

There are a number of highly conserved photosystem II light-harvesting antenna proteins in moss whose functions are unclear. Here, we investigated the involvement of chlorophyll-binding proteins, Lhcb6 and Lhcb5, in light-harvesting and photosynthesis regulation in Physcomitrella patens. Lhcb6 or Lhcb5 knock-out resulted in a disordered thylakoid arrangement, a decrease in the number of grana membranes, and an increase in the number of starch granule. The absence of Lhcb6 or Lhcb5 did not noticeably alter the electron transport rates. However, the non-photochemical quenching activity in the lhcb5 mutant was dramatically reduced when compared to wild-type or lhcb6 plants under abiotic stress. Lhcb5 plants were more sensitive to photo-inhibition, while lhcb6 plants showed little difference compared to the wild-type plants under high-light stress. Moreover, both mutants showed a growth malformation phenotype with reduced chlorophyll content in the gametophyte. These results suggested that Lhcb6 or Lhcb5 played a unique role in plant development, thylakoid organization, and photoprotection of PSII in Physcomitrella, especially when exposed to high light or osmotic environments.

scholarly journals Papain-like cysteine proteases are required for the regulation of photosynthetic gene expression and acclimation to high light stress

2021 ◽  
Vol 72 (9) ◽  
pp. 3441-3454
Author(s):  
Sarah Alomrani ◽  
Karl J Kunert ◽  
Christine H Foyer
Keyword(s):  
Gene Expression ◽  
Large Subunit ◽  
Light Stress ◽  
D1 Protein ◽  
Carbon Assimilation ◽  
Cysteine Proteases ◽  
High Light ◽  
High Light Stress ◽  
Photosynthetic Gene ◽  
Transgenic Lines

AbstractChloroplasts are considered to be devoid of cysteine proteases. Using transgenic Arabidopsis lines expressing the rice cystatin, oryzacystatin I (OC-I), in the chloroplasts (PC lines) or cytosol (CYS lines), we explored the hypothesis that cysteine proteases regulate photosynthesis. The CYS and PC lines flowered later than the wild type (WT) and accumulated more biomass after flowering. In contrast to the PC rosettes, which accumulated more leaf chlorophyll and carotenoid pigments than the WT, the CYS lines had lower amounts of leaf pigments. High-light-dependent decreases in photosynthetic carbon assimilation and the abundance of the Rubisco large subunit protein, the D1 protein, and the phosphorylated form of D1 proteins were attenuated in the CYS lines and reversed in the PC lines relative to the WT. However, the transgenic lines had higher amounts of LHC, rbcs, pasbA, and pasbD transcripts than the WT, and also showed modified chloroplast to nucleus signalling. We conclude that cysteine proteases accelerate the reconfiguration of the chloroplast proteome after flowering and in response to high-light stress. Inhibition of cysteine proteases, such as AtCEP1, slows chloroplast protein degradation and stimulates photosynthetic gene expression and chloroplast to nucleus signalling, enhancing stress tolerance traits.

scholarly journals Quantitative spatial and temporal assessment of regulatory element activity in zebrafish

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Shipra Bhatia ◽  
Dirk Jan Kleinjan ◽  
Kirsty Uttley ◽  
Anita Mann ◽  
Nefeli Dellepiane ◽  
...  
Keyword(s):  
Disease Risk ◽  
Regulatory Element ◽  
Regulatory Elements ◽  
Wild Type ◽  
Enhancer Activity ◽  
Fluorescent Reporter ◽  
Dual Reporter ◽  
Transgenic Lines ◽  
Element Activity ◽  
The Impact

Mutations or genetic variation in noncoding regions of the genome harbouring cis-regulatory elements (CREs), or enhancers, have been widely implicated in human disease and disease risk. However, our ability to assay the impact of these DNA sequence changes on enhancer activity is currently very limited because of the need to assay these elements in an appropriate biological context. Here, we describe a method for simultaneous quantitative assessment of the spatial and temporal activity of wild-type and disease-associated mutant human CRE alleles using live imaging in zebrafish embryonic development. We generated transgenic lines harbouring a dual-CRE dual-reporter cassette in a pre-defined neutral docking site in the zebrafish genome. The activity of each CRE allele is reported via expression of a specific fluorescent reporter, allowing simultaneous visualisation of where and when in development the wild-type allele is active and how this activity is altered by mutation.

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