scholarly journals Potential of Grasses in Phytolith Production in Soils Contaminated with Cadmium

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 109 ◽  
Author(s):  
Múcio Mágno de Melo Farnezi ◽  
Enilson de Barros Silva ◽  
Lauana Lopes dos Santos ◽  
Alexandre Christofaro Silva ◽  
Paulo Henrique Grazziotti ◽  
...  

Cadmium (Cd) is a very toxic heavy metal occurring in places with anthropogenic activities, making it one of the most important environmental pollutants. Phytoremediation plants are used for recovery of metal-contaminated soils by their ability to absorb and tolerate high concentrations of heavy metals. This paper aims to evaluate the potential of grasses in phytolith production in soils contaminated with Cd. The experiments, separated by soil types (Typic Quartzipsamment, Xanthic Hapludox and Rhodic Hapludox), were conducted in a completely randomized design with a distribution of treatments in a 3 × 4 factorial scheme with three replications. The factors were three grasses (Urochloa decumbens, Urochloa brizantha and Megathyrsus maximus) and four concentrations of Cd applied in soils (0, 2, 4 and 12 mg kg−1). Grass growth decreased and increased Cd concentration in shoots of grasses with the increased Cd rates in soils. The toxic effect of Cd resulted in production and Cd occlusion in phytoliths produced in shoots of the grasses. Grasses showed potential for phytolith production, independent of soil type, providing phytoextraction of Cd in phytoliths. Megathyrsus maximus was the grass with the highest tolerance to Cd, evidenced by higher production and Cd capture in phytoliths for the evaluated soils. Phytolith production by grasses in Cd-contaminated soils is related to genetic and physiological differences of the evaluated grasses and Cd availability in soils.

2021 ◽  
Vol 9 (2) ◽  
pp. 85-88
Author(s):  
Muhammad Ahsan Qureshi ◽  
Waqar Shafqat

Shoot tips of newly emerging mango shoots were used as an explant and cultured on MS media for direct shooting. Different plant growth regulators i.e. BAP, NAA and IAA in varying concentrations were added to basal MS media. Experiment was laid out according to Completely Randomized Design (CRD) with four treatments and each treatment was replicated thrice. Maximum shoot induction (55.56%) was observed with 1 mg/L of BAP. Maximum mortality (83.30%) was observed when maximum concentration of BAP (3 mg/L) was used. Minimum days (12.633) to induce shoots were observed on MS media supplemented with 1 mg/L of BAP. Maximum number of shoots (2.50) were recorded in MS media supplemented with 1 mg/L of BAP. Low concentration of NAA initiated roots earlier in regenerated shoots as compare to high concentrations of NAA and IAA. MS media supplemented with NAA (1 mg/L) took less days (21 days) to induce roots. The combination of auxins (MS+ NAA 3 mg /L + IAA 1 mg /L) proved the best for root induction (38.87%). Keywords: Mangifera indica, tropical fruits, asexual propagation, plant tissue culture, micropropagation, phenolic exudation, acclimatization.


2019 ◽  
Vol 37 ◽  
Author(s):  
M.J. KHAN ◽  
N. AHMED ◽  
W. HASSAN ◽  
T. SABA ◽  
S. KHAN ◽  
...  

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.


2021 ◽  
Vol 1 (01) ◽  
pp. 1-12
Author(s):  
Adi Fathul Qohar ◽  
Eko Hendarto ◽  
Munasik ◽  
Nur Hidayat ◽  
Bahrun ◽  
...  

ABSTRAK Penelitian dengan metode eksperimental yang bertujuan untuk mengkaji pengaruh kombinasi dosis pemupukan kompos organik dan penambahan azolla terhadap pertumbuhan rumput raja (Pennisetum purpureophoides). Penelitian telah dilakukan pada tanggal 1 April sampai 30 September 2020 terletak di lahan pertanian, Desa Beji, Kecamatan Kedungbanteng, Kabupaten Banyumas. Percobaan menggunakan rancangan acak lengkap pola faktorial dengan faktor pertama adalah dosis pupuk kompos organik yakni 10, 20, dan 30 ton ha-1, dan penambahan azolla yakni 10, 20, 30 persen dari faktor pertama sehingga dihasilkan 9 kombinasi perlakuan. Setiap kombinasi perlakuan diulang sebanyak 3 kali sehingga menghasilkan 27 satuan percobaan. Tanaman rumput raja pada pemotongan kedua ditanam pada lahan seluas 400 meter persegi dengan jarak tanam 80 x 40 cm. Pupuk kompos organik yang digunakan berasal dari kotoran sapi potong yang telah selesai proses pengomposan, dan azolla berasal dari lahan persawahan. Parameter penelitian yang diukur adalah tinggi tanaman, dan diameter batang yang diukur pada hari ke 14, 28, dan 42 hari. Data yang telah diperoleh kemudian dianalisis menggunakan analisis variansi (ANOVA) dan dilanjutkan dengan uji orthogonal polinomial. Hasil menunjukan bahwa pemberian dosis kompos berpengaruh sangat nyata (P<0,01) terhadap tinggi tanaman dan diameter batang, sedangkan pemberian pupuk azolla juga berpengaruh sangat nyata (P<0,01) terhadap tinggi tanaman dan diameter batang, akan tetapi interaksi tidak berpengaruh nyata (P>0,05). Pemberian dosis pupuk kompos organik dapat digunakan untuk mencapat pertumbuhan rumput raja yang terbaik sebesar 30 ton ha-1 dan penambahan azolla sebesar 30 persen dari dosis pupuk organik. Kata kunci: Azolla, Kompos, Pertumbuhan, Rumput Raja ABSTRACT Research with experimental method aims to examine the effect of combination dose of organic compost fertilization and the addition of Azolla on the growth of king grass (Pennisetum purpureophoides). From April 1 to September 30, 2020, research was conducted on agricultural land, Beji Village, Kedungbanteng District, Banyumas Regency. The experiment used a completely randomized design with a factorial pattern with the first factor being the dose of organic compost, namely 10, 20, and 30 tons ha-1, and the addition of Azolla, namely 10, 20, 30 percent of the first factor, resulting in 9 treatment combinations. Each treatment combination was repeated three times to produce 27 experimental units. The king grass plant in the second cutting was planted on ??400 square meters with a spacing of 80 x 40 cm. The organic compost used is derived from beef cattle dung that has finished the composting process, and Azolla comes from rice fields. Research parameters measured were plant height and stem diameter measured on days 14, 28, and 42 days. The data that had been obtained were then analyzed using analysis of variance (ANOVA) and continued with the orthogonal polynomial test. The results showed that the dose of compost had a very significant effect (P<0.01) on plant height and stem diameter, while the application of Azolla fertilizer also had a very significant effect (P<0.01) on plant height and stem diameter, but the interaction had no effect significant (P>0.05). Dosage of organic compost can be used to achieve the best king grass growth of 30 tons ha-1 and the addition of Azolla by 30 percent of the dose of organic fertilizer. Keywords: Azolla, Compost, Growth, King Grass


2018 ◽  
Vol 36 (0) ◽  
Author(s):  
Y. GRAAT ◽  
A.L. BACHA ◽  
M.P. NEPOMUCENO ◽  
P.L.C.A. ALVES

ABSTRACT: This study aimed at evaluating the effects of different desiccation periods of Urochloa decumbens on the initial development of Eucalyptus urograndis (clone C-219H). The experiment was conducted in 100 liter concrete boxes, previously filled with Dark-Red Latosol. U. decumbens was sown in all plots, except for the control sample without covering. After 95 days from sowing, the herbicide glyphosate (dose of 1,424 g a.e. ha-1) was sprayed at intervals of 0 (Apply-Plant), 7, 14, 21, 28 and 35 days before eucalyptus planting, plus a control sample without covering, totaling seven treatments. A completely randomized design with six replications was used. Eucalyptus plants were evaluated 30, 60 and 90 days after planting (DAP), when the height of the plants and the diameter of the stem were determined. At the end of the experimental period (90 DAP), the leaf area and the dry matter of leaves and stem were measured. It is possible to conclude that the Apply-Plant modality was harmful to some of the eucalyptus characteristics (E. urograndis - C-219H), while planting the seedlings in periods over 14 days after the desiccation of U. decumbens plants promoted a better development of the culture.


2018 ◽  
Vol 36 (1) ◽  
pp. 77-82
Author(s):  
Maria MS Wiethan ◽  
Gabriel S Bortolin ◽  
Renata S Pinto ◽  
Antonio Carlos F Silva

ABSTRACT The study aimed to evaluate the initial development, as well as, the chemical characteristics of lettuce plants grown in substrate with high concentrations of bioagent (trichoderma). A completely randomized design with four replicates was used. The treatments, applied to a substrate composed of a blend of vermicompost and commercial substrate, were established by the following doses of the commercial product ICB Nutrissolo Trichoderma: T1) 0.0; T2) 1.0; T3) 2.0; T4) 4.0; T5) 8.0 and T6) 16.0x1011 conidia kg-1 of product. After inoculation, the substrate was placed in trays, where 50 lettuce seeds were sown on each replication. Plants were thinned after emergency stabilization, maintaining ten seedlings per tray. At 28 days after sowing, the characteristics percentage and emergence speed index, leaf area, fresh and dry leaf biomass, volume, length and surface area of the root were evaluated. We also determined the chemical characteristics of the leaves. The percentage of seed germination and the emergence speed index were lower at doses higher than 4.0x1011 conidia kg-1 of the biological product. All the studied doses influenced negatively in shoot and root development of the plants. The percentage of nutrients in the leaves was higher in treatments with ICB.


2015 ◽  
Vol 33 (2) ◽  
pp. 193-201 ◽  
Author(s):  
F.P. Carvalho ◽  
C.A.D. Melo ◽  
M.S. Machado ◽  
D.C.F.S. Dias ◽  
E.M. Alvarenga

This study aimed to evaluate the allelopathic effect of various concentrations of an aqueous extract of eucalyptus leaves on Urochloa decumbens and Panicum maximum seeds. The extract was prepared from Eucalyptus urograndis leaves that were milled and mixed with distilled water in a 1:9 milled leaves: water ratio to obtain an extract with a defined concentration of 100%. In addition, dilutions of 50%, 25% and 12.5% were prepared, and a 0% dilution was used as a control. The experiment followed a completely randomized design, with four replicates, each of 50 seeds of U. decumbens and 50 seeds of P. maximum, arranged on filter paper moistened with each concentration of extract in a Gerbox plastic box. The results demonstrated the allelopathic potential of E. urograndis aqueous extracts applied to the seeds of U. decumbens and P. maximum. The 50% and 100% concentrations of leaf extract most strongly inhibited the germination, vigor and seedling growth of U. decumbens and P. maximum. The germination speed index and the root length were the characteristics that were most affected by the potentially allelopathic substances contained in the eucalyptus extracts at all concentrations.


2020 ◽  
Vol 13 (1) ◽  
pp. 30-37
Author(s):  
Ika Ratna Agustin ◽  
Yunianta ◽  
Teti Estiasih

Cowpea (Vigna unguiculata L. Walp) has a protein content of 22,9% to obtain the protein in high concentrations, is made as a concentrates or isolates protein. The aim of study to determine of chemical and functional properties of flour and cowpea protein concentrates on their solubility. The study uses a completely randomized design with precipitation method based on solvent, there is distilled water, 5% salt solution, alkaline solution (NaOH 0,5 N) and ethanol 70%. The result showed that cowpea protein fractions of albumin, globulin, glutelin and prolamin had different minimum and maximum solubility. Flour and cowpea protein concentrates had different chemical composition and functional properties. Flour and cowpea protein concentrates can be develop in various food product


Author(s):  
Daniel Abreu Vasconcelos CAMPELO ◽  
Isis Silva GONÇALVES ◽  
Isamaira Costa e SILVA ◽  
Lorena Batista DE MOURA ◽  
Marcos Ferreira BRABO ◽  
...  

ABSTRACT The present study aimed to evaluate the influence of garlic essential oil (GEO) on the growth performance and morphometric variables of severum (Heros severus) post-larvae. A growth trial was performed using 150 severum post-larvae (1.6 ± 0.03 mg and 5.58 ± 0.1 mm), distributed in fifteen 1-L aquaria, with constant aeration. The fish were essayed in a completely randomized design with five treatments, using diets containing graded levels of GEO (0.00, 0.05, 0.50, 1.00 and 1.50 g kg-1) and three replicates. The diets were offered four times a day, until apparent satiation, for 30 days. At the end of the experiment, the fish were counted, weighed and measured, to evaluate growth performance and morphometric variables. Increased diet levels of GEO resulted in a linear reduction in growth performance parameters, except for the survival rate, weight and length uniformity of the batch, and morphometric variables which did not present significant differences. Elevated concentrations of dietary GEO may be detrimental to larvae and post-larvae of fish, which could be more sensitive to the potential damage that high concentrations of this essential oil may cause. Therefore, the inclusion of GEO in levels above 0.05 g kg-1 in diets for severum post-larvae is not recommended.


2019 ◽  
Vol 37 ◽  
Author(s):  
L. TROPALDI ◽  
I.P.F.S. BRITO ◽  
R.C. DIAS ◽  
M.L.B. TRINDADE ◽  
C.A. CARBONARI ◽  
...  

ABSTRACT: Maintenance of straw on the soil surface in sugarcane production areas can influence weed occurrence and herbicide dynamics. After application, considerable losses of clomazone can occur as a result of its physicochemical characteristics. For this reason, novel formulations have been developed. In addition to the conventional formulation, microencapsulated formulations are currently available. Thus, the aim of this study was to observe and compare the effect of clomazone formulations under different application conditions. For this purpose, the experiment was carried with 12 treatments in a completely randomized design, with four replications, using clomazone formulations (1,200 g a.i. ha-1), on soils with different moisture levels, in the presence and absence of sugarcane straw, followed or not by rainfall simulation. The treatments were compared by observation of number of emerged plants and phytotoxicity at 7 and 14 DAA, as well shoot dry matter at 14 DAA for Ipomoea nil, Urochloa decumbens and Panicum maximum. In general, the microencapsulated formulation presented a better performance under the less suitable application conditions when compared to the conventional one. However, for the other conditions, both formulations had a similar performance.


2020 ◽  
Vol 13 ◽  
pp. 117862211989846 ◽  
Author(s):  
Ahmadreza Yazdanbakhsh ◽  
Seyed Nadali Alavi ◽  
Seyed Alireza Valadabadi ◽  
Fatemeh Karimi ◽  
Zainab Karimi

Heavy metals are among the most critical environmental pollutants close to industrial areas. One example is the cultivated fields in the south of Alborz industrial city in Iran, which is irrigated by treated industrial wastewater. It is contaminated by heavy metals and irrigation with wastewater treatment plants effluent, which made it salty. In this study, the application of 2 amendments, biosolids and cow manure, in improving the heavy metal accumulation in the ornamental sunflower from these types of soils was investigated. A greenhouse experiment using a completely randomized design with 4 replications and applying cow manure and biosolids in 3 weight ratios (6%, 12%, 25%) was conducted to evaluate the efficiency of sunflower in removing Pb, Ni, and Zn from the soil. Adding the amendments increased the rate of germination by 50% to 176%. Although the simultaneous utilization of cow manure in high ratios with biosolids and cow manure with low biosolids decreased the sunflower survival, nonetheless, the simultaneous addition of these organic amendments could increase the survival rate in other treatments. Moreover, the plants’ biomass was increased by adding modifiers such as cow manure and biosolids. The results showed that in treatments with 2 modifiers, the remediation factor of Pb, Zn, and Ni has increased 83.7 to 95.5, 78.4 to 87.5, and 74.9 to 94.9, respectively, in comparison to the control one. Therefore, we conclude that adding biosolids and cow manure simultaneously could improve the ornamental sunflower ability to accumulate heavy metals.


Sign in / Sign up

Export Citation Format

Share Document