Physiological state of the sterlet × beluga hybrid (Asipenser ruthenus × Huso huso) when growing under ecological conditions in the Volga delta

2020 ◽  
pp. 41-52
Author(s):  
Svetlana Sergeevna Astafyeva ◽  
Alexander Robertovich Lozovskiy
Keyword(s):  
Serum Albumin ◽  
Total Protein ◽  
Physiological State ◽  
Serum Triglyceride ◽  
Blood Parameters ◽  
The State ◽  
Physiological Adaptation ◽  
Huso Huso ◽  
Volga Delta ◽  
Iron Analysis

Three-year-olds (15 individuals) and eight-year-olds (15 individuals) of the sterlet × beluga hybrid (Asipenser ruthenus × Huso huso), grown in mesh cages in the Volga delta, were studied in the third decade of September 2019 in order to establish the functional state of the organism by blood parameters reflecting the state erythron and metabolism. The results of the performed study found that the erythron system differs in a smaller number of blood erythrocytes in eight-year-olds (0,76 ± 0,04 × 1012l –1) compared with three-year-olds (0,98 ± 0,04 × 1012l –1), in the absence of differences in blood hemoglobin and serum iron. Analysis of the state of protein metabolism showed that the content of total protein and the activity of ASAT in serum were the same in the groups. The serum albumin content in eight-yearolds was 17,87 ± 0,92 g/l, which was significantly less than in three-year-olds (24,15 ± 1,78) (p < 0,05). The relative content of serum albumin (as a percentage of total protein) in eightyear-olds was 51,04 ± 3,12%, which is less than in three-year-olds (72,51 ± 6,04%) (p < 0.01). The serum ALAT activity in eight-year-olds was lower than in three-year-olds (2,33 ± 0,16 and 2,93 ± 0,17 μkat/l, respectively) (p < 0,05). The results of the study of lipid metabolism revealed that serum cholesterol in eight-year-olds (2,62 ± 0,11 mmol/l) is less than in three-year-olds (1,39 ± 0,05) (p < 0,05). However, the serum triglyceride content did not differ between threeyear-olds and eight-year-olds, being 3,50 ± 0,07 and 3,26 ± 0,13 mmol/l, respectively. The glycemic values in three-year-olds and eight-year-olds are significantly different and amount to 1,80 ± 0,08 and 1,42 ± 0,11 mmol/l, respectively (p < 0.05). The results of the study can be used to assess the state of physiological adaptation of the hybrid and to determine the reference values of the parameters of the erythron system and metabolic homeostasis.

scholarly journals Metabolic status of rabbits under physiological adaptation to cage husbandry technology

2021 ◽  
Vol 37 ◽  
pp. 00047
Author(s):  
Natalia Khokhlova ◽  
Vladimir Semenyutin ◽  
Viktor Eremenko
Keyword(s):  
Alkaline Phosphatase ◽  
Structural Changes ◽  
Total Bilirubin ◽  
Physiological State ◽  
Blood Parameters ◽  
Physiological Adaptation ◽  
Adaptation Process ◽  
Plantar Surface ◽  
Regulatory Processes ◽  
Hind Limbs

The article considers changes in biochemical blood parameters depending on the degree of adaptation process manifesting on the plantar surface of hind limbs of different sex rabbits and physiological state under cage husbandry technology. Groups of stud bucks (1st series), pregnant and lactating does (2nd and 3rd series of experiments) were formed according to the stages of structural changes depending on the stress of physiological regulatory processes of adaptation (the systematics proposed by R.M. Baevsky). The authors studied the following factors in serum: total protein, protein fractions (albumin, α-, β-, γ-globulin), creatinine, total bilirubin, urea, uric acid, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, glucose triacylglycerol and cholesterol. At the stressed stage of regulatory systems the group of bucks showed a reduction of total bilirubin on the background of decrease of ALT, AST and alkaline phosphatase, which can testify to activation of liver function at this stage, and increase in bilirubin, ALT, AST with a simultaneous decrease in alkaline phosphatase, noted at the overstress stage of physiological adaptation regulatory processes, can testify to decrease in the functional activity of hepatocytes.Pregnant rabbits have the most pronounced compensatory reactions in the third stage of the adaptation process: the increase in absolute and relative γ-globulin values indicates the activation of immune factors. An increase in creatinine and urea levels during the gestation period, against a background of developing adaptive changes on the plantar surface of the feet, indicates a decrease in the filtration capacity of the renal tubules.Lactating animals experience an increased physiological load on the liver, which is expressed by an increase in triacylglycerols throughout the adaptation.

Vitamin D Provision in High-Yield Dairy Cows During the Winter Housing Period

2014 ◽  
Vol 1 (1) ◽  
pp. 42-46
Author(s):  
L. Yuskiv ◽  
V. Vlizlo
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase Level ◽  
Mineral Metabolism ◽  
Physiological State ◽  
Blood Parameters ◽  
Postnatal Period ◽  
High Yield ◽  
Oral Supplementation ◽  
Calcium Phosphorus ◽  
Vitamin D 3

Aim. To investigate the vitamin D status in highly productive cows during winter housing period and effect of cholecalciferol by various ways of vitamin D 3 injection to cows in last days of gestation and after calving. Methods. Enzyme-linked immunoassay, spectrophotometry. Results. It has been stated that intramuscular injection of cholecalciferol into cows caused increase of the vitamin D 3 active metabolite – 25-OHD 3 , calcium, phosphorus and magnesium levels together with decrease of alkaline phosphatase level in pre- and post-natal periods. Oral supplementation makes little infl uence on the studied blood parameters of cows. Conclusions. Extrabuccal administration and oral supplementation of cholecalciferol in winter housing period to high-yield cows in the last days of gestation and after calving is accompanied by increased levels of its metabolites and their effect on mineral metabolism in the postnatal period. The nature of these changes depends on the mode of vitamin D administration and the physiological state of the cows.

scholarly journals IMMUNOLOGIC TOLERANCE AFTER SPECIFIC IMMUNIZATION

1964 ◽  
Vol 120 (3) ◽  
pp. 435-447 ◽  
Author(s):  
Marianne M. Dorner ◽  
Jonathan W. Uhr
Keyword(s):  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Antibody Response ◽  
Serum Albumin ◽  
Human Serum ◽  
Bovine Serum ◽  
The State ◽  
Immunologic Tolerance ◽  
Secondary Antibody

Specific immunologic tolerance to bovine serum albumin (BSA) was induced in approximately one-half of the rabbits that had been primarily immunized and were prepared for a secondary antibody response to BSA. The state of tolerance lasted for several months in the majority of rabbits and was not easily terminated by immunization with human serum albumin followed by BSA.

scholarly journals PHYSIOLOGICAL STATE AND PRODUCTIVITY OF ANIMALS UNDER THERMAL STRESS

2019 ◽  
Vol 51 (3) ◽  
pp. 26-31
Author(s):  
E.A. Azhmuldinov ◽  
◽  
Yu.N. Chernyshenko ◽  
M.G. Titov ◽  
◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Live Weight ◽  
Blood Parameters ◽  
Maximum Temperature ◽  
Total Plasma ◽  
Ambient Temperatures ◽  
Study Results ◽  
Total Plasma Protein ◽  
Negative Effect

A study was conducted of the effect of heat stress on the clinical and physiological state of male rabbits at the age of 5 months with an average live weight of 1551 g. The maximum temperature the animals were exposed to during the experiment was +42 °C. The study results showed a decrease in feed intake and an increase in water consumption, which contributed to a decrease in live weight. Hematological parameters, including hemoglobin, erythrocytes, leukocytes, total plasma protein, globulins and cholesterol increased during the action of this stress factor. It was found that the effect of high ambient temperatures on rabbits negatively affected their physiological functions; the negative effect was reflected by the morphological and biochemical blood parameters.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals DOSE-DIFFERENTIATED CORRELATION INTERACTIONS BETWEEN INDICES OF HEMOGLOBIN EXCHANGE IN TURKEYS UNDER THE INFLUENCE OF THE PROBIOTIC FORMULATION VETOM 1.2

2020 ◽  
pp. 82-91 ◽  
Author(s):  
M. S. Yakovleva ◽  
N. S. Yakovleva ◽  
N. A. Gotovchikov ◽  
S. N. Tishkov ◽  
L. P. Ermakova
Keyword(s):  
Serum Albumin ◽  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Bacillus Amyloliquefaciens ◽  
Total Bilirubin ◽  
Inverse Correlation ◽  
Direct Bilirubin ◽  
Subsequent Decrease ◽  
Probiotic Preparation

The effect of the probiotic preparation Vetom 1.2 on the basis of Bacillus subtilis VKPM B-10641, Bacillus amyloliquefaciens VKPM B-10642 and Bacillus amyloliquefaciens VKPM B-10643 in different doses on the correlation interactions between the parameters of turkey hemoglobin exchange, blood erythrocytes and blood red blood cells direct and total bilirubin, total protein and serum albumin was studies. Correlations were calculated using Spearman formula, the reliability of correlations was checked by Student. The formulation was used for turkeys once a day for 30 days in doses of 12.5; 25; fifty; 75 and 100 mg / kg body weight. It was found that Vetom 1.2 in minimal doses leads to an inverse correlation between iron and total bilirubin, direct bilirubin, total protein and albumin, an increase in the concentration of the formulation leads to the disappearance of this effect with a gradual subsequent decrease in this indicator. Vetom 1.2 in doses up to 50 mg / kg leads to an inverse correlation between the concentrations of hemoglobin and direct bilirubin, total protein and serum albumin, at a dose of 75 mg / kg there is a direct correlation between these indicators, and at a dose of 100 mg / kg returns a negative correlation. The probiotic preparation Vetom 1.2 leads to a direct dependence of the concentration of bilirubins (total and direct) in serum on the concentrations of hemoglobin and red blood cells when used in most doses, except 75 mg / kg, which reduces the correlation.

POST-SURGICAL CHANGES IN BLOOD PARAMETERS OF THE OVINE FETUS IN UTERO

1979 ◽  
Vol 59 (2) ◽  
pp. 265-271 ◽  
Author(s):  
D. D. KITTS ◽  
C. R. KRISHNAMURTI ◽  
W. D. KITTS
Keyword(s):  
Physiological State ◽  
Amino Nitrogen ◽  
Blood Parameters ◽  
In Utero ◽  
Base Parameters ◽  
Intrauterine Surgery ◽  
Ovine Fetus ◽  
Plasma Metabolite ◽  
Daily Changes

A surgical procedure for the catheterization of the external saphenous vein of the 100-to 120-day-old ovine fetus in utero has been described. Daily changes in blood acid-base parameters and plasma metabolite and cortisol concentrations were measured in vivo to determine the physiological state of the ovine fetus following intrauterine surgery. Though blood gas values returned to stable values the day following surgery, plasma glucose, lactate and alpha amino nitrogen and betahydroxybutyrate levels took 5 days to do so. It is concluded that a minimum of 5 days should elapse following surgery before metabolic experiments are undertaken.

Functional state of the organism of the hybrid Russian sturgeon × Siberian sturgeon (Acipenser gueldenstaedtii × Acipenser baerii) after winter in cages in Volga Delta

2021 ◽  
pp. 60-69
Author(s):  
Svetlana Sergeevna Astafyeva ◽  
Alexander Robertovich Lozovskiy
Keyword(s):  
Blood Glucose ◽  
Carbohydrate Metabolism ◽  
Functional State ◽  
Total Protein ◽  
Siberian Sturgeon ◽  
Russian Sturgeon ◽  
Acipenser Baerii ◽  
Glucose Content ◽  
Volga Delta ◽  
Acipenser Gueldenstaedtii

Two-year-olds (15 individuals) and five-year-olds (15 individuals) of the Russian sturgeon × Siberian sturgeon (Acipenser gueldenstaedtii × Acipenser baerii) hybrid, kept in net cages in the Volga delta, were studied after wintering in order to establish the functional state of the organism according to the complex of indicators of the erythron system and metabolism substances. The results of the performed study found that the erythron system in five-year-olds is characterized by a higher content of erythrocytes and blood hemoglobin (0.58 ± 0.01×10 12 l –1 and 64.98 ± 1.29 g/l, respectively), compared with two-year-olds (0.49 ± 0.02×10 12 l –1 and 64.98 ± 1.29 g/l, respectively).The serum iron content in both groups was the same. A higher content of total protein and albumin in the blood, as well as a greater activity of ALT were found in five-year-olds, compared with two-year-olds. Thus, the content of total protein and albumin in blood serum in five-year-olds was at the level of 48.23 ± 1.54 and 21.58 ± 2.34 g/l, respectively, and in two-year-olds — 32.42 ± 0.92 and 13,84 ± 0.61 g/l, respectively (p < 0.05). The activity of serum ALT in five-year-olds was 1.07 ± 0.06 μkat/l, and in two-year-olds — 0.85 ± 0.03 μkat/l (p < 0.01). Serum cholesterol in five-year-olds (3.94 ± 0.04 mmol/l) and two-year-olds (4.4 ± 0.08 mmol/l) did not differ significantly (p > 0.05). The content of serum triglycerides in five-year-olds (4.50 ± 0.49 mmol/l) and in two-year-olds (5.36 ± 0.45 mmol/l) did not differ significantly (p > 0.05). The study of carbohydrate metabolism revealed a pronounced variability in the glycemic index in the younger age group (coefficient of variation 44.7 %) with an average blood glucose value of 2.69 ± 0.31 mmol/l. The blood glucose content of five-year-olds was higher than that of two-year-olds — 3.84 ± 0.24 mmol/l (p < 0.05). The established parameters of variation and the age specificity of the indicators of the erythron system, protein, lipid and carbohydrate metabolism can be used to assess the state of physiological adaptation of the hybrid after wintering in cages in the Volga delta.

Influence of feed additive Vitartil on the productive traits and biochemical parameters of steers’ blood

2021 ◽  
pp. 25-32
Author(s):  
S. S. Mirgalimova ◽  
A. O. Trufanova ◽  
R. R. Fatkullin
Keyword(s):  
Physiological State ◽  
Relative Number ◽  
Blood Parameters ◽  
The Body ◽  
Biologically Active ◽  
Feed Additive ◽  
Control Group ◽  
Vital Functions ◽  
Productive Traits ◽  
Experimental Group

The physiological state of animals is to a certain extent characterized by hematological indicators, since blood occupies a special place in the body. By transferring nutrients and biologically active substances, the blood performs the general regulation of vital functions of the body. The use of Vitartil as a feed additive in the diets of animals leads to an increase in glycolytic processes and a decrease in the body’s need for oxygen delivery, which contributes to the growth of the animal body. The purpose of the research was to study the changes in blood parameters taking into account the age of Hereford steers against the background of the use of Vitartil feed additive to the main diet and its effect on the productive traits of animals. In order to carry out the experiment two groups of steers-analogs of Hereford breed have been formed at the age of 6 months per 10 heads in each. The difference between the groups was that the steers of the experimental group have been received the feed additive Vitartil in addition to the main diet. The greater yield of the meat part was in the steers of the experimental group in comparison with the herdmates of the control group. This indicator in the experimental group was 78,9 %, while in the control group it was lower by 1,6 %. The animals of the experimental group were superior to their herdmates in terms of meat weight by 1,4 kg or by 8,8 %. The relative number of bones was lower in the experimental group and amounted to 18,4 %, and in the control group 19,2 %. The meat content index was high in the steer have been received the biologically active additive Vitartil and was 4,3, and in the control group 4,0. It has been found that the use of Vitartil feed additive to the main diet of Hereford steers increases the blood content of hemoglobin and glucose, which has a certain effect on the morphological and chemical composition of meat, as well as increases its energy value.

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